Alpha-fetoprotein triggers hepatoma cells escaping from immune surveillance through altering the expression of Fas/FasL and tumor necrosis factor related apoptosis-inducing ligand and its receptor of lymphocytes and liver cancer cells

Figure 1 Influence of AFP (20 mg/L), anti-AFP (40 mg/L), AFP (20 mg/L) plus anti-AFP (40 mg/L) and HSA (20 mg/L) on the expression of Fas and FasL protein in Bel7402 cells and Jurkat cells. A, C: Western blot of Fas and FasL protein of Bel7402 cells or of Jurkat cells; B, D: Quantitative analysis of IOD of Fas/IOD of actin, the columns represent mean±SD.

Figure 2 Effect of AFP (20 mg/L), anti-AFP (40 mg/L) and AFP (20 mg/L) plus anti-AFP (40 mg/L) on the expression of Fas and FasL protein in Bel7402 cells or in Jurkat when the cells were cultured individually or co-cultured. A and C: Western blot analysis of Fas and FasL protein of Bel7402 cells or of Jurkat cells; B and D: Quantitative analysis IOD of Fas/IOD of actin, the columns represent mean±SD.

Figure 3 Effect of AFP (20 mg/L), anti-AFP (40 mg/L) and AFP (20 mg/L) plus anti-AFP (40 mg/L) on the TRAIL mRNA expression of Bel7402 cells and Jurkat cells were cultured individually or cultured together. A: Northern blot of TRAIL mRNA of Bel7402 cells and Jurkat cells; B: Quantitative analysis IOD of TRAIL/IOD of β-actin, the columns represent mean±SD.

Figure 4 Influence of AFP (20 mg/L), anti-AFP (40 mg/L) and AFP (20 mg/L) plus anti-AFP (40 mg/L) on the expression of TRAILR mRNA in Bel7402 cells and Jurkat cells were cultured individually or cultured together. A: Northern blot analysis of TRAILR mRNA of Bel7402 cells and Jurkat cells; B: Quantitative analysis IOD of TRAILR/IOD of β-actin, the columns represent mean±SD.