Inhibitory effect of human telomerase antisense oligodeoxyribonucleotides on the growth of gastric cancer cell lines in variant tumor pathological subtype

Figure 1 Results of quantification determination of telomerase activity. Lane 1: negative control of lysis buffer; lane 2: normal human tissue of gastric mucosa; lane 3: gastric cancer; lane 4: MKN-28 cell line; lane 5: SGC-7901 cell line; lane 6: MKN-45 cell line; lane 7: positive control of 0.1 amol R8.

Figure 2 Effect of antisense oligomers on telomerase activity. Lane 1: negative control of lysis buffer; lanes 2-4: 5 μmol/L ASODN* on MKN-28, SGC-7901 and MKN-45 cell lines; lanes 5-7: 10 μmol/L ASODN on MKN-28, SGC-7901 and MKN-45 cell lines; lane 8: negative control of dealing with RNase A; lanes 9-11: 10 μmol/L NODN* on MKN-28, SGC-7901 and MKN-45 cell lines; lane 12: positive control of 0.1 amol R8. *ASODN: antisense oligomer and NODN: missense oligomer.

Figure 3 Effect of oligomers on viability of MKN-28, SGC-7901 and MKN-45 cells.

Figure 4 Effect of oligomers on viability of SGC-7901 cells at different time points and concentrations. A represents antisense telomerase oligodeoxyribonucleotides (ASOND) and N represents missense oligodeoxyribonucleotides (NOND).

Figure 5 Light microscopy investigation of MKN-45 and SGC-7901 cells after in vitro treatment with different oligomers (10 μmol/L, 96 h). A: contrast group (10×15); B: antisense oligomer group (10×15); C: non-antisense oligomer group (10×15).

Figure 6 Electron microscopy investigation of cell apoptosis of MKN-45 and SGC-7901 cells after in vitro treatment with different oligomers (10 μmol/L, 96 h). A: contrast group (×2300); B: antisense group (×6400); C: non-antisense group (×3800).

Figure 7 TUNEL test of cell apoptosis of SGC-7901 and MKN-45 gastric cancer cells after in vitro treatment with different oligomers (10 μmol/L, 96 h). A: negative control (20×15); B: positive control (20×15); C: antisense group (20×15); D: non-antisense group (20×15).