Identification of effective siRNA against K-ras in human pancreatic cancer cell line MiaPaCa-2 by siRNA expression cassette

Figure 1 Apoptosis analysis of cells interfered by siRNA by FACS. A: Normal cells; B: Cells transfected without DNA; C: Cells interfered by nonspecific siRNA; D: Cells interfered by site 194 siRNA; E: Cells interfered by site 491 siRNA; F: Cells interfered by site 327 siRNA.

Figure 2 Detection of the inhibition of K-ras mRNA expression in MiaPaCa-2 cells by RT-PCR. Marker DNA2000 Marker; Lane 1: Normal cells; Lane 2: Cells transfected without DNA; Lane 3: Cells interfered by nonspecific siRNA; Lane 4: Cells interfered by site 194 siRNA; Lane 5: Cells interfered by site 491 siRNA; Lane 6: Cells interfered by site 327 siRNA; β-actin is a control.

Figure 3 Detection of inhibition of K-ras protein expression in MiaPaCa-2 cells by immunofluorescence. A: Normal cells; B: Cells interfered by site 327 siRNA; C: Cells interfered by site 194 siRNA; D: Cells interfered by site 491 siRNA.