Effects of folic acid on epithelial apoptosis and expression of Bcl-2 and p53 in premalignant gastric lesions

Figure 1 Effect of folic acid on the cell cycle and the induction of apoptosis DNA content was determined by flow cytometry after ethanol fixation, permeabilization and PI staining. A: before; B: after folic acid treatment. Representative results of one of the subjects are shown.

Figure 2 Annexin V-FITC/propidium iodide double staining of gastric epithelium before and after treatment with folic acid The proportion of apoptosis was measured by flow cytometry using Annexin V-FITC and PI. In the figures, X-axis (FL1-H) indicates Annexin V, Y-axis (FL2-H) indicates PI. A: before; B: after folic acid treatment. Representative results of one of the subjects are shown.

Figure 3 Flow cytometry analysis of Bcl-2 and p53 expression in a patient before and after folic acid treatment For Bcl-2 and p53 expression, cells were stained with FITC-labeled monoclonal anti-Bcl-2 (A and B) or anti-p53 antibodies (C and D); and then measured by flow cytometry. A and C: before; B and D: after folic acid treatment. Representative results of one of the subjects are shown.