Liver Cancer
Copyright ©2005 Baishideng Publishing Group Inc.
World J Gastroenterol. Mar 14, 2005; 11(10): 1420-1425
Published online Mar 14, 2005. doi: 10.3748/wjg.v11.i10.1420
Figure 1
Figure 1 Proliferation assays showing cell numbers from mean counts of three experiments. Cells were treated as described in Materials and Methods with sirolimus, tacrolimus, or the combination of both. Sir = sirolimus; Tac = tacrolimus. Data are expressed as mean±SE. (A: SK-Hep 1: aP = 0.0105; bP = 0.0156; cP = 0.254; dP<0.0001; B: Hep 3B: eP<0.0001; fP = 0.0654; gP = 0.0002; hP<0.0001).
Figure 2
Figure 2 Cell-cycle analysis of both cell lines after treatment for 24 h with sirolimus, tacrolimus, or the combination of both at a dose of 25 ng/mL each compound. M1 = Sub-G1 region, indicating cells with small DNA fragments, a typical feature of apoptosis; M2 = G1-Phase; M3 = S-Phase; M4 = G2/M-Phase. The different cell cycle phases (M1-M4) were set in a reference analysis and kept constant throughout the measurements.
Figure 3
Figure 3 Western blot analysis of p53 and bcl-2. SK-Hep 1 cells express wild-type p53. Hep 3B cells harbor a deletion of the p53 gene and express no p53 protein. Expression levels were analyzed by densitometry referring to levels of actin. Relative values to controls are shown.