Coexpression of cholecystokinin-B/gastrin receptor and gastrin gene in human gastric tissues and gastric cancer cell line

Figure 1 Expression of CCK-B receptor (below) and gastrin (above) mRNA in human gastric tissues and gastric cancer cell line. Total RNA extracted from matched tumor (C1, C2) and non-neoplastic (N1, N2) gastric tissues of patients with gastric cancer, inflammatory gastric mucosa (I1 and I2) from patients with gastritis, normal stomachs from two autopsied patients (A1, A2) and SGC-7901 cells (7901) were analyzed by RT-PCR. The second lane (C) corresponds to the negative control (H₂O). The results are representatives of all specimens. Cck-br and gastrin stand for cholecystokinin B receptor and gastrin gene, respectively. β-actin: β-actin gene. M: marker (bp).

Figure 2 Semi-quantitative analysis of CCK-B receptor expres-sion in human gastric tissues. The expression levels of CCK-B receptors were higher in normal group than in malignant and inflammatory groups, but there was no significant difference compared with other groups. Cck-br/β-actin: ratio of the in-tensities of the bands of CCK-B receptor vsβ-actin on agarose gel. The results are expressed as mean ± SD in every group.

Figure 3 Sequence analyses of CCK-B receptor/gastrin mRNA from samples. L08112.1: Human brain CCK-B/gastrin receptor gene from GenBank. NM_000805: Homo sapiens gastrin gene from GenBank. Cck-br and gastrin: CCK-B receptor and gastrin gene sequences from specimens, respectively. The results showed are representatives of four samples.