Quantifying anti-HBV effect of targeted ribonuclease by real-time fluorescent PCR

Figure 1 HBV DNA concentration in the supernatants of tranfected and transducted 2. 2.15 cells. A: HBV DNA concen-tration in the supernatants of transfected 2.2.15 cells. TN, TNm, hEDN, HBVc, pcDNA3.1 (-), and MOCK represent the super-natants collected from 2.2.15 cells transfected by p/TN, p/TNm, p/hEDN, p/HBVc, pcDNA3.1 (-), or mock solution (DMEM plus Lipofectamine^TM 2000 reagent containing no plasmid), respectively. B: HBV DNA concentrations in the supernatants of transducted 2.2.15 cells. TAT-TN, TAT-TNm, TAT-hEDN, TAT-HBVc, and MOCK represent the supernatants collected from 2.2.15 cells transducted by purified proteins of TAT-TN, TAT-TNm, TAT-hEDN, TAT-HBVc, or mock solution (DMEM), respectively.