Hepatic differentiation capability of rat bone marrow-derived mesenchymal stem cells and hematopoietic stem cells

Figure 1 A-D Cell morphology before and after differentiation (Original magnification, 200 ×). A: Fibroblast-like MSC before differentiation, B: Round MSC after differentiation, C: Freshly isolated Thy1.1⁺ cells, D: Thy1.1⁺ cells 10 d after differentiation.

Figure 2 Phenotype analysis of MSCs and Thy-1. 1⁺ cells. A: Thy-1.1 of mononuclear bone marrow cells before MACS; B, C, D: Thy-1.1, CD45, CD34 after MACS purification; E, F, G: Thy-1.1, CD45, CD34 of MSCs.

Figure 3 RT-PCR analysis of hepatic gene expression before and after differentiation. A: Analysis of CK18 mRNA before differentiation, lane 1: Adult liver, lane 2: Mononuclear BM cells.

Figure 4 Immunocytofluorescence. A: MSCs-albumin, B: Thy1.1⁺ cells-CK18, C: Thy1.1⁺ cells-AFP.