Cytochrome C oxidase III interacts with hepatitis B virus X protein in vivo by yeast two-hybrid system

Figure 1 Result of detection of X gene in yeasts transformed with pAS2-1-X. M: PCR marker; lanes 1 and 2: transformed yeasts; lanes 3: untransformed yeasts; N: negative control.

Figure 2 Western blot analysis of expressed fusion protein in yeasts transformed with PAS2-1-X. Lane 1: protein moclecular weight standard; lane 2: protein extracted from transformed yeasts; lane 3: protein extracted from untransformed yeasts.

Figure 3 Results of amplification for cDNA fragment by PCR from the positive clones. M: 100 bp DNA ladder; lane1: positive clone; N: negative control.

Figure 4 Mating experiment for the interaction between cox III and X protein in yeast cells.