Liver Cancer
Copyright ©The Author(s) 2004.
World J Gastroenterol. May 15, 2004; 10(10): 1421-1424
Published online May 15, 2004. doi: 10.3748/wjg.v10.i10.1421
Figure 1
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Figure 1 Restriction enzyme digestion of recombinant plas-mid pcDNA3. 1-mAST by Xba I and Hind III. Lane 1: DNA/EcoR I + Hind III Marker; Lanes 2, 3, 5, 6: positive clones; Lanes 4, 7: negative clones.
Figure 2
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Figure 2 Morphology of HCC cells. A: SMMC-7721 cells; B: SMCC-7721 cells transfected with pcDNA3.1 (+); C: SMCC-7721 cells transfected with pcDNA3.1-mAST.
Figure 3
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Figure 3 Growth curve of SMCC-7721 cells.
Figure 4
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Figure 4 RT-PCR of transfected cells. Lane 1: DNA/EcoR I + Hind III Markers; Lane 2: positive clones.
Figure 5
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Figure 5 Western blotting of angiostatin expression. Lane 1: Marker protein; Lanes 2, 3: SMMC-7721/pcDNA3.1-mAST; Lane 4: SMMC-7721/pcDNA3.1 (+); Lane 5: SMMC-7721.
Figure 6
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Figure 6 Growth curve of tumors.
Figure 7
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Figure 7 Immunohistochemical analysis of MVD in tumor tissue (× 200). A: blank control group; B: angiostatin group