Cooperative inhibitory effects of antisense oligonucleotide of cell adhesion molecules and cimetidine on cancer cell adhesion

Figure 1 Effect of ASON on expression of E-selectin and ICAM-1 in endothelial cells (^aP < 0. 01, vs group II, n = 5). I: Basal, II: TNF-α, III: cimetidine, IV: lipo-ASON, V: lipo-ASON/cimetidine.

Figure 2 Agarose gel electrophoresis of RT-PCR products. M:Marker, 1: E/basal, 2: E/TNF-α, 3: E/cimetidine, 4: E/lipo-ASON, 5: E/lipo-ASON/cimetidine, 6: I/basal, 7: I/TNF-α, 8: I/cimetidine, 9: I/lipo-ASON, 10: I/cimetidine.

Figure 3 Relative expression value of ICAM-1 and E-selectin to β-actin by RT-PCR. I: Basal, II: TNF-α, III: Cimetidine, IV: Lipo-ASON, V: Lipo-ASON/Cimetidine.