Effect of Qingyitang on activity of intracellular Ca2+ -Mg2+ -ATPase in rats with acute pancreatitis

Figure 1 Histopathological findings in AP rats before and after treatment, HE stains. A: Morphological changes in pancreatic tissue of AP rats before treatment, × 200. B: Morphological changes in pancreatic tissue of AP rats after treatment with QYT, × 200. C: Morphological changes in hepatic tissue of AP rats before treatment, × 200. D: Morphological changes in hepatic tissue of AP rats after treatment with VitE, × 200. E: Morphological changes in renal tissue of AP rats before treatment, × 400. F: Morphological changes in hepatic tissue of AP rats after treatment with Tet, × 400.

Figure 2 Enzyme histochemistry staining for intracellular Ca²⁺ -Mg²⁺ -ATPase in AP rats before and after treatment. A: Pancreatic tissue in AP rats before treatment, × 400. B: Pancreatic tissue in AP rats after treatment with QYT, × 400. C: Hepatic tissue in AP rats before treatment, × 200. D: Hepatic tissue in AP rats after treatment with VitE, × 200. E: Renal tissue in AP rats before treatment, × 400. F: Renal tissue in AP rats after treatment with Tet, × 400.

Figure 3 Expression of Ca²⁺ - Mg²⁺ - ATPase in AP rats before and after treatment analyzed by RT-PCR. A: Expression of Ca²⁺ -Mg²⁺ - ATPase mRNA in pancreatic tissue of different groups. B: Expression of Ca²⁺ -Mg²⁺ -ATPase mRNA in hepatic tissue of different groups. C: amplification product of GAPDH gene in pancreatic tissue of different groups. D: amplification product of GAPDH geneinhepatic tissue of different groups.Note: 1: AP 1 h, 2: AP 5 h, 3: QYT + AP 1 h, 4: QYT + AP 5 h, 5: Normal control group, 6: Tet + AP1 h, 7: Tet + AP 5 h, 8: VitE + AP 1 h, 9:VitE + AP 5 h, M: PCR marker.