Original Article
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World J Gastroenterol. Sep 7, 2010; 16(33): 4135-4144
Published online Sep 7, 2010. doi: 10.3748/wjg.v16.i33.4135
Design of 16S rRNA gene primers for 454 pyrosequencing of the human foregut microbiome
Carlos W Nossa, William E Oberdorf, Liying Yang, Jørn A Aas, Bruce J Paster, Todd Z DeSantis, Eoin L Brodie, Daniel Malamud, Michael A Poles, Zhiheng Pei
Carlos W Nossa, William E Oberdorf, Michael A Poles, Department of Medicine, New York University School of Medicine, New York, NY 10016, United States
Liying Yang, Department of Pathology, New York University School of Medicine, New York, NY 10016, United States
Jørn A Aas, Bruce J Paster, Department of Molecular Genetics, The Forsyth Institute, Boston, MA 02115, United States
Jørn A Aas, Faculty of Dentistry, University of Oslo, PO Box 1052 Blindern, 0316 Oslo, Norway
Bruce J Paster, Harvard School of Dental Medicine, Boston, MA 02115, United States
Todd Z DeSantis, Eoin L Brodie, Lawrence Berkeley National Laboratory, Center for Environmental Biotechnology, Berkeley, CA 94720, United States
Daniel Malamud, New York University College of Dentistry, New York, NY 10016, United States
Zhiheng Pei, Department of Pathology and Medicine, New York University School of Medicine, New York, NY 10016, United States; Department of Veterans Affairs New York Harbor Health System, New York, NY 10010, United States
Author contributions: Pei Z and Nossa CW designed this study; Nossa CW, Oberdorf WE, Yang L and Aas JA performed computational analyses; Nossa CW, Pei Z, Paster BJ, DeSantis TZ, Brodie EL, Malamud D and Poles MA participated in manuscript preparation.
Supported by (in part) Grants UH2CA140233 from the Human Microbiome Project of the NIH Roadmap Initiative and National Cancer Institute; R01AI063477 from the National Institute of Allergy and Infectious Diseases; DE-11443 from the National Institute of Dental and Craniofacial Research; and U19DE018385 from the National Institute of Dental & Craniofacial Research
Correspondence to: Zhiheng Pei, MD, PhD, Department of Veterans Affairs New York Harbor Health System, 423 E 23rd Street, New York, NY 10010, United States. zhiheng.pei@nyumc.org
Telephone: +1-212-9515492 Fax: +1-212-2634108
Received: March 12, 2010
Revised: April 16, 2010
Accepted: April 23, 2010
Published online: September 7, 2010
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