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World J Gastroenterol. Mar 21, 2009; 15(11): 1359-1366
Published online Mar 21, 2009. doi: 10.3748/wjg.15.1359
Published online Mar 21, 2009. doi: 10.3748/wjg.15.1359
Ex-vivo evaluation of gene therapy vectors in human pancreatic (cancer) tissue slices
Michael A van Geer, Conny T Bakker, Ronald PJ Oude Elferink, Piter J Bosma, Liver Center, Academic Medical Center of the University of Amsterdam, Meibergdreef 69/71, 1105 BK Amsterdam, The Netherlands
Koert FD Kuhlmann, Department of Surgery, Academic Medical Center of the University of Amsterdam, Meibergdreef 9, 1105BK Amsterdam, The Netherlands
Fibo JW ten Kate, Department of Pathology, Academic Medical Center of the University of Amsterdam, Meibergdreef 9, 1105 BK Amsterdam, The Netherlands
Author contributions: van Geer MA generated the Adenovirus preparations and performed the transduction experiments in the slices; Kuhlmann KFD provided the resection specimens and performed the immune histological stainings; Bakker CT produced the Lentivirus and part of the adenovirus preparations and performed the slicing of the resection specimens; ten Kate FJW scored the viability of the slices after ex-vivo culture; Oude Elferink RPJ designed the study and obtained the grant required for performing this study and corrected the paper; Bosma PJ designed the study, generated the AAV vector and wrote the paper.
Correspondence to: Dr. Piter J Bosma, Liver Center, Academic Medical Center of the University of Amsterdam, Meibergdreef 69/71, 1105 BK Amsterdam, The Netherlands. p.j.bosma@amc.uva.nl
Telephone: +31-20-5668850
Fax: +31-20-5669190
Received: December 26, 2008
Revised: February 23, 2009
Accepted: March 2, 2009
Published online: March 21, 2009
Revised: February 23, 2009
Accepted: March 2, 2009
Published online: March 21, 2009
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