The present research aims to evaluate the efficacy of Silibinin-loaded mesoporous silica nanoparticles (Sil@MSNs) immobilized into polylactic-co-glycolic acid/Collagen (PLGA/Col) nanofibers on the in vitro proliferation of adipose-derived stem cells (ASCs) and cellular senescence. Here, the fabricated electrospun PLGA/Col composite scaffolds were coated with Sil@MSNs and their physicochemical properties were examined by FTIR, FE-SEM, and TGA. The growth, viability and proliferation of ASCs were investigated using various biological assays including PicoGreen, MTT, and RT-PCR after 21 days. The proliferation and adhesion of ASCs were supported by the biological and mechanical characteristics of the Sil@MSNs PLGA/Col composite scaffolds, according to FE- SEM. PicoGreen and cytotoxicity analysis showed an increase in the rate of proliferation and metabolic activity of hADSCs after 14 and 21 days, confirming the initial and controlled release of Sil from nanofibers. Gene expression analysis further confirmed the increased expression of stemness markers as well as hTERT and telomerase in ASCs seeded on Sil@MSNs PLGA/Col nanofibers compared to the control group. Ultimately, the findings of the present study introduced Sil@MSNs PLGA/Col composite scaffolds as an efficient platform for long-term proliferation of ASCs in tissue engineering.

Exclusive physicochemical and biological properties of carbon allotrope graphene have attracted the peer attention of researchers for the synthesis and development of newer topical remedies including films, scaffolds, microspheres, and hydrogels. Here, graphene nanoplatelets (GN) were embedded into a different ratio of polymeric ERL100/ERS100 solution and fabricated in the form of a scaffold through the electrospinning process. FTIR spectra displayed characteristic similar peaks present both in GN and GN-loaded scaffold owing to the compatibility of GN and polymeric mixture. XRD curve revealed a distinct GN peak at nearly 26° whereas from DSC/TGA thermal stability was observed between polymers and graphene nanoplatelets. FESEM images showed ultrathin architecture of GN-loaded scaffold in a range of 280 ± 90 nm. The fabricated scaffold exhibited hydrophilicity (contact angle 48.8 ± 2.8°) and desirable swelling index (646% in skin pH media) which were desired criteria for the scaffold for topical application. In vitro, antifungal activity was conducted through the broth microdilution method against different virulent dermatophytes i.e., Microsporum gypseum, M. canis, M. fulvum, and Trychophyton rubrum. For in vivo evaluation, T. rubrum inoculum was applied on the dorsal surface of each group of Swiss albino mice, and the degree and intensity of mycelial growth or erythema on skin surfaces was visually investigated. The study depicted complete signs of cure after 14 days of application of G3-loaded scaffold on the infected dorsal site. Hence graphene-loaded scaffold represented a possible alternative for the treatment of topical fungal infections caused by dermatophytes.

The use of electrospun meshes has been proposed as highly efficient protective equipment to prevent respiratory infections. Those infections can result from the activity of micro-organisms and other small dust particles, such as those resulting from air pollution, that impair the respiratory tract, induce cellular damage and compromise breathing capacity. Therefore, electrospun meshes can contribute to promoting air-breathing quality and controlling the spread of such epidemic-disrupting agents due to their intrinsic characteristics, namely, low pore size, and high porosity and surface area. In this review, the mechanisms behind the pathogenesis of several stressors of the respiratory system are covered as well as the strategies adopted to inhibit their action. The main goal is to discuss the performance of antimicrobial electrospun nanofibers by comparing the results already reported in the literature. Further, the main aspects of the certification of filtering systems are highlighted, and the expected technology developments in the industry are also discussed.

The lung is a vital organ that houses the alveoli, which is where gas exchange takes place. The COVID-19 illness attacks lung cells directly, creating significant inflammation and resulting in their inability to function. To return to the nature of their job, it may be essential to rejuvenate the afflicted lung cells. This is difficult because lung cells need a long time to rebuild and resume their function. Biopolymeric particles are the most effective means to transfer developing treatments to airway epithelial cells and then regenerate infected lung cells, which is one of the most significant symptoms connected with COVID-19. Delivering biocompatible and degradable natural biological materials, chemotherapeutic drugs, vaccines, proteins, antibodies, nucleic acids, and diagnostic agents are all examples of these molecules' usage. Furthermore, they are created by using several structural components, which allows them to effectively connect with these cells. We highlight their most recent uses in lung tissue regeneration in this review. These particles are classified into three groups: biopolymeric nanoparticles, biopolymeric stem cell materials, and biopolymeric scaffolds. The techniques and processes for regenerating lung tissue will be thoroughly explored.

Nanofibers prepared by biobased materials are widely used in the field of biomedicine, owing to outstanding biocompatibility, biodegradable characters, and excellent mechanical behavior. Herein, we fabricated multilayered nanofibrous scaffolds in order to improve the performance of drug delivery. The composite layer-by-layer scaffolds were incorporated by hydrophobic poly(l-lactic acid) (PLA): polycaprolactone (PCL) and hydrophilic poly(vinyl alcohol) (PVA) nanofibers via multilayer electrospinning. Morphological and structural characteristics of the developed scaffolds measured by scanning electron microscopy (SEM), and transmission electron microscopy (TEM) confirmed smooth and uniform fibers ranging in nanometer scale. The differences in contact angles and Fourier transform infrared spectrum (FTIR) between single-layered PVA nanofibers and multilayered scaffolds verified the existence of PLA: PCL surface. In vitro biodegradable and drug release analysis depicted multilayered scaffolds had good biodegradability and potential for medical application. Due to the model drug incorporation, scaffolds exhibited good antibacterial activity against Escherichia coli and Staphylococcus aureus by the zone of inhibition test. These results revealed that the multilayered scaffolds were proved to be desirable antibacterial materials for biomedical application.

Electrospun nanofibres can outperform their melt-blown counterparts in many applications, especially air filtration. The different filtration mechanisms of nanofibres are particularly important when it comes to the air filtration of viruses (such as COVID-19) and bacteria. In this work, we present an electrospun nanofibre filter media, FilterLayrTM by NanoLayr Ltd., containing poly(methyl methacrylate)/ethylene vinyl alcohol nanofibres. The outstanding uniformity of the nanofibres was indicated by the good correlation between pressure drop (ΔP) and areal weight with R2 values in the range of 0.82 to 0.98 across various test air velocities. By adjusting the nanofibre areal weight (basis weight), the nanofibre filter media was shown to meet the particle filtration efficiency and breathability requirements of the following internationally accepted facemask and respirator standards: N95 respirator facemask performance in accordance with NIOSH 42CFR84 (filtration efficiency of up to 98.10% at a pressure drop of 226 Pa and 290 Pa at 85 L·min-1 and 120 L·min-1, respectively), Level 2 surgical facemask performance in accordance with ASTM F2299 (filtration efficiency of up to 99.97% at 100 nm particle size and a pressure drop of 44 Pa at 8 L·min-1), and Level 2 filtration efficiency and Level 1 breathability for barrier face coverings in accordance with ASTM F3502 (filtration efficiency of up to 99.68% and a pressure drop of 133 Pa at 60 L·min-1), with Level 2 breathability being achievable at lower nanofibre areal weights.

One of the main protective measures against COVID-19's spread is the use of face masks. It is therefore of the utmost importance for face masks to be high functioning in terms of their filtration ability and comfort. Notwithstanding the prevalence of the commercial polypropylene face masks, its effectiveness is under contention, leaving vast room for improvement. During the pandemic, the use of at least one mask per day for each individual results in a massive number of masks that need to be safely disposed of. Fabricating biodegradable filters of high efficiency not only can protect individuals and save the environment but also can be sewed on reusable/washable cloth masks to reduce expenses. Wearing surgical masks for long periods of time, especially in hot regions, causes discomfort by irritating sensitive facial skin and warmed inhaled air. Herein, we demonstrate the fabrication of novel electrospun composites layers as face mask filters for protection against pathogens and tiny particulates. The combinatorial filter layers are made by integrating TiO₂ nanotubes as fillers into chitosan/poly(vinyl alcohol) polymeric electrospun nanofibers as the outer layer. The other two filler-free layers, chitosan/poly(vinyl alcohol) and silk/poly(vinyl alcohol) as the middle and inner composite layers, respectively, were used for controlled protection, contamination prevention, and comfort for prolonged usage. The ASTM standards evaluation tests were adopted to evaluate the efficacy of the assembled filter, revealing high filtration efficiency compared to that of commercial surgical masks. The TiO₂/Cs/PVA outer layer significantly reduced Staphylococcus aureus bacteria by 44.8% compared to the control, revealing the dual effect of TiO₂ and chitosan toward the infectious bacterial colonies. Additionally, molecular dynamics calculations were used to assess the mechanical properties of the filter layers.