Hu W, Shi ZH, Ma TF, Yu JP. Effects of Ginkgo biloba extract on liver fibrosis induced by carbon tetrachloride in rats.
Shijie Huaren Xiaohua Zazhi 2004;
12:886-891. [DOI:
10.11569/wcjd.v12.i4.886]
[Citation(s) in RCA: 1] [Impact Index Per Article: 0.0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Indexed: 02/06/2023] Open
Abstract
AIM: To study the effects of Ginko biloba extract (EGb) on hepato-fibrosis induced by carbon tetrachloride (Cl4) in rat model.
METHODS: Rat liver fibrosis model was induced by Cl4 administration. Wistar rats were randomly divided into 5 groups: normal control group, a model group, a interven-tional group, a therapeutic group, and a EGb group. The EGb interventional group, apart from administration of Cl4, was treated concurrently with EGb 0.3 g/kg, ig once a day; the EGb therapeutic group was treated with EGb 0.3 g/kg, ig once a day after cirrhosis was induced successfully, and the EGb group was treated only with EGb 0.3 g/kg, ig once a day. At the end of wk 8 and 16, all the rats were sacrificed. The pathological changes of liver were observed by H-E and Von-Gieson staining.The expression of mRNA and proteins of collagen I/TGF1 in liver were determined by RT-PCR and immunohistochemistry.
RESULTS: The degree of liver fibrosis and level of mRNA and proteins of collagen I/TGF1 in liver were significantly reduced in the EGb interventional and therapeutic groups compared with those in the model group (type I collagen mRNA: 0.0 778±0.054 vs 0.2 361±0.113, 0.1 075±0.007 vs 0.2 361±0.113, P < 0.01; type I collagen proteins: 0.2 563±0.0 009 vs 0.2 885±0.0 025, 0.2 541±0.0 076 vs 0.2 885±0.0 025 , P < 0.01; TGF1 mRNA: 0.523±0.015 vs 0.956±0.049 , 0.524±0.009 vs 0.956±0.049, P < 0.01; TGF1 proteins: 0.2 785±0.0 012 vs 0.3 015±0.0 012, 0.2 791±0.0 016 vs 0.3 015±0.0 012, P < 0.01).The EGb group had the same results as the normal control group.
CONCLUSION: EGb has prophylactic and therapeutic effects on CCl4-induced rat liver fibrosis, probably through its anti-lipoperoxidation, suppressing the activation of hepatic stellate cells and transition and reducing the synthesis of hepatic type I collagen and TGF1.
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