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Al-Adwan SM, Al-Qaisi TS, Jabbar AAJ, Amin KYM, Sami HF, Althagbi HI, Al-Dabhawi AH, Wahab BAA, Hassan RR, Abdulla MA, Saleh MI. Field Marigold (Calendula arvensis L.) accelerates wound-healing in vivo: role of transforming growth factor-beta1 (TGF-β1), inflammatory, and biochemical molecules. J Mol Histol 2025; 56:156. [PMID: 40381088 DOI: 10.1007/s10735-025-10433-3] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 03/01/2025] [Accepted: 04/20/2025] [Indexed: 05/19/2025]
Abstract
Medicinal plants are major sources of natural products, which have gained a renewed interest in recent years because of drawbacks associated with synthetic ones used for human health disorders. Calendula arvensis L. is a traditional medicinal plant used for many inflammatory-related diseases. The study explores the acute toxicity and wound-healing effects of methanolic extracts of Calendula arvensis L. aerial parts (MECAA) on excisional neck injury in rats. A two-week acute toxicity procedure was applied to find the safety of MECAA in animal models. For the wound-healing experiment, a uniform dorsal neck injury was created for twenty-four Sprague-Dawley rats, which were aligned arbitrarily into 4 groups and received topical treatments; group A rats addressed with normal saline; group B rats had intrasite gel; groups C and D rats had 0.2 ml of 250 and 500 mg/kg of MECAA, respectively. There was no toxicity nor mortality in rats supplemented orally with up to 5 g/kg MECAA. MECAA topical addressing accelerated wound contraction represented by higher deposition of fibroblast and keratinocytes, angiogenic factors, and reduced inflammatory cells. MECAA treatment up-regulated tissue antioxidants (SOD and CAT), transforming growth factor-β 1, and hydroxyproline (collagen) contents while lowering MDA and serum inflammatory mediators (TNF-α and IL-6). The outcomes can serve as scientific evidence for the traditional use of Calendula arvensis as a wound healer, which requires further molecular isolation and identification as a viable source of a potent drug formulation.
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Affiliation(s)
- Safwan Mahmoud Al-Adwan
- Department of Cosmetic Science, Faculty of Allied Medical Sciences, Al-Ahliyya Amman University, Amman, 19328, Jordan
| | - Talal Salem Al-Qaisi
- Department of Biomedical Sciences, College of Health Sciences, Abu Dhabi University, P.O. Box 59911, Abu Dhabi, United Arab Emirates
| | - Ahmed A J Jabbar
- Department of Medical Laboratory Technology, Erbil Technical Health and Medical College, Erbil Polytechnic University, Erbil, 44001, Iraq.
| | - Kamaran Younis M Amin
- Department of Chemistry, College of Education, Salahaddin University-Erbil, Erbil, 44001, Iraq
| | - Hawri Fatih Sami
- Department of Medical Biochemical Analysis, Cihan University-Erbil, Erbil, Kurdistan Region, Iraq
| | | | | | - Bassam Ali Abed Wahab
- Department of Physiology, Biochemistry and Pharmacology, Faculty of Vet Medicine, University of Kufa, Kufa, Iraq
| | - Rawaz Rizgar Hassan
- Department of Medical Laboratory Science, College of Science, Knowledge University, Kirkuk Road, Erbil, 44001, Iraq
| | - Mahmood Ameen Abdulla
- Department of Medical Analysis, Faculty of Applied Science, Tishk International University, Erbil, 44001, Iraq
| | - Musher Ismael Saleh
- Department of Chemistry, Faculty of Science and Health, Koya University, Koya KOY45, Erbil, Kurdistan Region, 44001, Iraq
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Abahuni Ucar M, Gul EM, Uygunoz D, Derun EM, Piskin MB. Investigation of PVA Matrix Hydrogel Loaded with Centaurea cyanus Extract for Wound Dressing Applications: Morphology, Drug Release, Antibacterial Efficiency, and In Vitro Evaluation. Gels 2025; 11:264. [PMID: 40277700 PMCID: PMC12026772 DOI: 10.3390/gels11040264] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Grants] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 03/10/2025] [Revised: 03/21/2025] [Accepted: 03/24/2025] [Indexed: 04/26/2025] Open
Abstract
A polyvinyl alcohol (PVA) matrix hydrogel loaded with Centaurea cyanus extract was created for transdermal wound healing. Secondary metabolites, antibacterial properties, and the cytotoxicity of C. cyanus extract were investigated. The secondary metabolite profiles of the extract were determined by liquid chromatography-mass spectrometry (LC-MS) technique. It was determined that the extract has metabolites such as quinic acid, caffeoylquinic acid, kaempferol, etc., which contribute to the steps of wound healing. The extract had significant activity against Staphylococcus aureus when compared with ampicillin antibiotic and showed an inhibition zone of 16.9 mm ± 0.8, whereas ampicillin's inhibition zone was 15.8 mm ± 0.8. The extract did not exhibit cytotoxic effects on 3T3-L1 (CL173) healthy skin fibroblasts, maintained cell viability for 72 h, and exhibited a 19% proliferative effect. Fourier-transform infrared spectroscopy, scanning electron microscopy, ultraviolet visible spectrophotometer, tensile strength analyses, in vitro release, and physicochemical tests were conducted. It was seen that the surfaces of the samples are smooth and homogeneous, patches had a significant amount of water absorption capacity, and 79% of the extract was released within the first 24 h of application; consequently, these results indicate that C. cyanus might be used in wound healing with its antibacterial, growth-promoting properties.
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Affiliation(s)
- Melis Abahuni Ucar
- Department of Bioengineering, Yildiz Technical University, Istanbul 38000, Turkey; (M.A.U.); (M.B.P.)
| | - Enis Muhammet Gul
- Department of Chemical Engineering, Yildiz Technical University, Istanbul 38000, Turkey; (E.M.G.); (D.U.)
| | - Deniz Uygunoz
- Department of Chemical Engineering, Yildiz Technical University, Istanbul 38000, Turkey; (E.M.G.); (D.U.)
| | - Emek Moroydor Derun
- Department of Chemical Engineering, Yildiz Technical University, Istanbul 38000, Turkey; (E.M.G.); (D.U.)
| | - Mehmet Burcin Piskin
- Department of Bioengineering, Yildiz Technical University, Istanbul 38000, Turkey; (M.A.U.); (M.B.P.)
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Kunjir H, Shetty L, Sharma DB, Aphale P, Gunturu S, Kunjir G, Chhatriwala AF. Comparative Evaluation of Bilateral Cleft Earlobe Rejuvenation with and without Calendula Officinalis 10% V/W- Randomised Control Trial. Indian J Otolaryngol Head Neck Surg 2025; 77:1248-1255. [PMID: 40093501 PMCID: PMC11909349 DOI: 10.1007/s12070-024-05312-4] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 11/13/2024] [Accepted: 12/26/2024] [Indexed: 03/19/2025] Open
Abstract
Calendula officinalis has anti microbial and anti inflammatory property and its usage for wound healing is the need of the hour. The ultimate aim of our research study was to evaluate and compare bilateral earlobe rejuvenation with and without Calendula officinalis. The purpose of the study was to examine the impact of calendula on wound healing and its effect in the duration of wound healing. This randomised control trial was conducted on patients with bilateral earlobe cleft and were advised for the treatment of earlobe repair. The technique used for repair was Cross Stitching Approach modified with subcutaneus suturing. The randomization for the calendula application and sequence was generated randomly by computer generated random numbering. Calendula ointment was placed in a double blinded manner by blinding the principal investigator and the statistician. Calendula ointment was applied every 12 hourly on one ear lobe for a period of 7 days and petroleum jelly was placed on the contralateral earlobe. Scar assessment was done using the Stony Brook Scar Evaluation Scale (SBSE) on 15th day postoperatively. The wound healing after earlobe repair in 35 patients was compared after 24 h, 7 days, and 15 days using an unpaired t test in this randomized control trial. Statistical significance was defined as a P-value of less than 0.01. The repeated measures ANOVA test was used to compare mean scores at different time intervals in groups 1 and 2. The Bon Feronic test was used to compare the mean score in groups 1 and 2 at different time intervals. Statistical significance was defined as a p-value of less than 0.01. The comparison of mean score on day 1 the (p value = 0.525), day 7 was (p value = 0.324) and day 15 which was clinically significant (p value < 0.001). Calendula ointment was found to be beneficial in wound healing after cleft earlobe repair in this study. Calendula ointment has demonstrated potential benefits in treating wounds in scheduled surgery with no post-operative problems, according to the findings of this study. This study sets the landmark for the use of Calendula ointment in the treatment of cleft earlobe rejuvenation in Maxillofacial surgery.
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Affiliation(s)
- Harshal Kunjir
- Department of Oral and Maxillofacial Surgery, Dr. D.Y. Patil Dental College and Hospital, Dr. D. Y. Patil Vidyapeeth, Pimpri, Pune 411018 India
| | - Lakshmi Shetty
- Department of Oral and Maxillofacial Surgery, Dr. D.Y. Patil Dental College and Hospital, Dr. D. Y. Patil Vidyapeeth, Pimpri, Pune 411018 India
| | - Dharmendra B. Sharma
- Department of Forensic Medicine and Toxicology, Dr. D. Y. Patil Homoeopathic Medical College and Research Centre, Dr. D. Y. Patil Vidyapeeth, Pimpri, Pune 411018 India
| | - Parth Aphale
- Department of Homoeopathic Pharmacy, Dr. D. Y. Patil College of Homoeopathic Medical College and Research Centre, Dr. D. Y. Patil Vidyapeeth, Pimpri, Pune 411018 India
| | - Srikanth Gunturu
- Department of Oral and Maxillofacial Surgery, Drs Sudha and Nageswara Rao Siddhartha Institute of Dental Sciences, Chinoutpalli, Gannavaram Mandal, Andhra Pradesh 521286 India
| | - Girija Kunjir
- Department of Oral and Maxillofacial Radiology, Dr. D.Y. Patil Dental College and Hospital, Dr. D. Y. Patil Vidyapeeth, Pimpri, Pune 411018 India
| | - Adnan F. Chhatriwala
- Department of Oral and Maxillofacial Surgery, Dr. D.Y. Patil Dental College and Hospital, Dr. D. Y. Patil Vidyapeeth, Pimpri, Pune 411018 India
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Khan KA, Durrani UF, Mahmood AK, Tipu MY, Fatima A, Saeed HA, Khalid AK, Ansari TS. Clinical study on wound healing properties of Nile tilapia fish skin as biological dressing in dogs. PLoS One 2025; 20:e0286864. [PMID: 40019884 PMCID: PMC11870337 DOI: 10.1371/journal.pone.0286864] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 10/14/2022] [Accepted: 10/02/2024] [Indexed: 03/03/2025] Open
Abstract
Frequency of clinical cases of dogs with massive skin losses is very high in urban areas of Pakistan following road accidents, sharp objects exposure and attack by other dog. These cases need intensive veterinary assistance for safe and speedy healing of wounds. Recently, skin of Nile tilapia fish (Oreochromis niloticus) is internationally gaining hype in medical field as biological dressing to boost dermatological reconstruction process. Nile tilapia skin is a recent research trend and a very limited research data is available on this topic for both human and animal subjects. This study was conducted at Department of Small Animal Clinical Sciences, University of Veterinary and Animal Sciences (UVAS), Lahore, Pakistan considering the wound healing promoter properties of Nile tilapia skin as a biological dressing for dogs with massive skin losses. Aim of this study was to evaluate Nile tilapia fish skin as wound healing promoter biological dressing following sutured and non-sutured application techniques. For this study 10 clinical cases of dogs were randomly selected as per set criteria and divided into groups A and B comprising 5 dogs each. Consent document was signed by each dog owner for volunteer participation in this study. Nile tilapia skin was collected from fresh subjects and treated with 10% povidone-iodine for 10-15 minutes to prepare biological dressing. In group A, biological dressing was sutured on wound (non-absorbable silk suture material) on the area with dermal loss. In group B, biological dressing was applied in a wrap manner on area of dermal loss without application of sutures. Wound healing was evaluated grossly and histologically on days 0, 7 and 14. Statistical analysis of comparison between groups A and B revealed that application of Nile tilapia skin derived biological dressing in wrap fashion results in fast and complication-free wound healing as compared to sutured tilapia biological dressing in dogs.
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Affiliation(s)
- Khizer Ahmed Khan
- Department of Small Animal and Clinical Sciences, University of Veterinary and Animal Sciences, Lahore, Pakistan
| | - Uzma Farid Durrani
- Department of Small Animal and Clinical Sciences, University of Veterinary and Animal Sciences, Lahore, Pakistan
| | - Asim Khalid Mahmood
- Department of Small Animal and Clinical Sciences, University of Veterinary and Animal Sciences, Lahore, Pakistan
| | - Muhammad Yasin Tipu
- Department of Pathology, University of Veterinary and Animal Sciences, Lahore, Pakistan
| | - Amber Fatima
- Department of Small Animal and Clinical Sciences, University of Veterinary and Animal Sciences, Lahore, Pakistan
| | - Hussain Ahmad Saeed
- Department of Small Animal and Clinical Sciences, University of Veterinary and Animal Sciences, Lahore, Pakistan
| | - Abdul Karim Khalid
- Department of Small Animal and Clinical Sciences, University of Veterinary and Animal Sciences, Lahore, Pakistan
| | - Tuba Shuja Ansari
- Department of Small Animal and Clinical Sciences, University of Veterinary and Animal Sciences, Lahore, Pakistan
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Possa GDOK, Chopek S, Pereira AV, Koga AY, de Oliveira MRP, Costa MDM. Calendula glycolic extract enhances wound healing of alginate hydrogel. Acta Cir Bras 2024; 39:e399724. [PMID: 39630705 PMCID: PMC11606611 DOI: 10.1590/acb399724] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 12/26/2023] [Accepted: 09/30/2024] [Indexed: 12/07/2024] Open
Abstract
PURPOSE To assess the cytotoxicity and wound healing properties of an alginate hydrogel containing calendula glycolic extract. METHODS Cell viability in murine fibroblasts (3T3 cells) was evaluated using MTT and SRB assays. The wound healing effect was tested in an incisional wound model on 50 female Wistar rats, divided into two groups: rats treated with alginate hydrogel (n = 25), and rats treated with calendula-alginate hydrogel. Wound healing was assessed by measuring wound retraction rate and histological analysis of lesion tissues over a 28-day period. Histological analyses were performed on days 2, 7, 14, 21, and 28 post-surgery to examine inflammatory infiltrate, macrophage count, and angiogenesis. Picrosirius red staining was used to compare the relative abundance of collagen types I and III fibers. RESULTS Cytotoxicity tests on 3T3 cells revealed increased cell viability with the calendula-alginate hydrogel. The calendula-alginate hydrogel also demonstrated a significant improvement in wound closure, supported by histopathological analysis, showing reduced inflammation, increased macrophage activity, and enhanced collagen deposition. CONCLUSIONS These findings evidenced the therapeutic potential of combining calendula extract and alginate for promoting enhanced wound healing.
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Affiliation(s)
| | - Solange Chopek
- Universidade Estadual de Ponta Grossa – Postgraduate Program in Biomedical Sciences – Ponta Grossa (PR) – Brazil
| | - Airton Vicente Pereira
- Universidade Estadual de Ponta Grossa – Postgraduate Program in Biomedical Sciences – Ponta Grossa (PR) – Brazil
- Universidade Estadual de Ponta Grossa – Department of Pharmaceutical Sciences – Ponta Grossa (PR) – Brazil
| | - Adriana Yuriko Koga
- Universidade Estadual de Ponta Grossa – Department of Medicine – Ponta Grossa (PR) – Brazil
| | - Marcia Regina Paes de Oliveira
- Universidade Estadual de Ponta Grossa – Department of Structural, Molecular and Genetic Biology – Ponta Grossa (PR) – Brazil
| | - Michele Dietrich Moura Costa
- Universidade Estadual de Ponta Grossa – Postgraduate Program in Biomedical Sciences – Ponta Grossa (PR) – Brazil
- Universidade Estadual de Ponta Grossa – Department of Structural, Molecular and Genetic Biology – Ponta Grossa (PR) – Brazil
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Raza A, Chohan TA, Zaidi SHH, Hai A, Alzahrani AR, Abida, Imran M, Saleem H. A Systematic Review on Biochemical Perspectives on Natural Products in Wound Healing: Exploring Phytochemicals in Tissue Repair and Scar Prevention. Chem Biodivers 2024; 21:e202400615. [PMID: 38958197 DOI: 10.1002/cbdv.202400615] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Grants] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 03/12/2024] [Revised: 06/20/2024] [Accepted: 06/28/2024] [Indexed: 07/04/2024]
Abstract
Wound healing is a critical process in tissue repair following injury, and traditional herbal therapies have long been utilized to facilitate this process. This review delves into the mechanistic understanding of the significant contribution of pharmacologically demonstrated natural products in wound healing. Natural products, often perceived as complex yet safely consumed compared to synthetic chemicals, play a crucial role in enhancing the wound-healing process. Drawing upon a comprehensive search strategy utilizing databases such as PubMed, Scopus, Web of Science, and Google Scholar, this review synthesizes evidence on the role of natural products in wound healing. While the exact pharmacological mechanisms of secondary metabolites in wound healing remain to be fully elucidated, compounds from alkaloids, phenols, terpenes, and other sources are explored here to delineate their specific roles in wound repair. Each phytochemical group exerts distinct actions in tissue repair, with some displaying multifaceted roles in various pathways, potentially enhancing their therapeutic value, supported by reported safety profiles. Additionally, these compounds exhibit promise in the prevention of keloids and scars. Their potential alongside economic feasibility may propel them towards pharmaceutical product development. Several isolated compounds, including chlorogenic acid, thymol, and eugenol from natural sources, are undergoing investigation in clinical trials, with many reaching advanced stages. This review provides mechanistic insights into the significant role of pharmacologically demonstrated natural products in wound healing processes.
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Affiliation(s)
- Ali Raza
- Institute of Pharmaceutical Sciences (IPS), University of Veterinary and Animal Sciences (UVAS), Lahore, Pakistan
| | - Tahir Ali Chohan
- Institute of Pharmaceutical Sciences (IPS), University of Veterinary and Animal Sciences (UVAS), Lahore, Pakistan
| | - Syeda Huma H Zaidi
- Department of Chemistry, Faculty of Science, Northern Border University, Arar, 91431, Saudi Arabia
| | - Abdul Hai
- Department of Medical Laboratory Technology, Faculty of Applied Medical Sciences, Northern Border University, Arar, 91431, Saudi Arabia
| | - Abdullah R Alzahrani
- Department of Pharmacology and Toxicology, Faculty of Medicine, Umm Al-Qura University, P.O. Box 13578, Al-Abidiyah, Makkah, 21955, Saudi Arabia
| | - Abida
- Department of Pharmaceutical Chemistry, College of Pharmacy, Northern Border University, Rafha, 91911, Saudi Arabia
| | - Mohd Imran
- Department of Pharmaceutical Chemistry, College of Pharmacy, Northern Border University, Rafha, 91911, Saudi Arabia
| | - Hammad Saleem
- Institute of Pharmaceutical Sciences (IPS), University of Veterinary and Animal Sciences (UVAS), Lahore, Pakistan
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Lanke H, Patadiya J, Banerjee B, Kandasubramanian B. Recent advancement and trends in the development of membranes having bactericidal attributes via direct ink writing. Biomed Mater 2024; 19:052003. [PMID: 39042104 DOI: 10.1088/1748-605x/ad66a4] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 02/07/2024] [Accepted: 07/23/2024] [Indexed: 07/24/2024]
Abstract
The necessity for orthopedic prostheses, implants, and membranes to treat diseases, trauma, and other disasters has increased as the risk of survive through various factors has intensified exponentially. Considering exponential growth in demand, it has been observed that the traditional technology of grafts and membranes lags to fulfill the demand and effectiveness simultaneously. These challenges in traditional methodologies prompted a revolutionary shift in the biomedical industry when additive manufacturing (AM) emerged as an alternative fabrication technique for medical equipments such as prostheses, implants, and membranes. However these techniques were fast and precise the major attributes of the biomedical materials were the processability, bactericidal nature, biocompatibility, biodegradability, and nontoxicity together with good mechanical properties. Major challenges faced by researchers in the present-day scenario regarding materials are the lack of bactericidal attributes in tailored material, though having better mechanical as well as biocompatible properties, which, on the other hand, are primary critical factors too, in the healthcare sector. Hence considering the advantages of AM and need for membranes with bacteriacidal attributes this present review will highlight the studies based on the manufacturing of membranes with bacteria-resistant properties majorly using direct ink writing and some AM techniques and the reasoning behind the antibacterial attributes of those composite materials.
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Affiliation(s)
- Himanshu Lanke
- Department of Chemical Engineering, All India Shree Shivaji Memorial Society's College of Engineering, Pune 411001, Maharashtra, India
| | - Jigar Patadiya
- Nano Surface Texturing, Department of Metallurgical and Materials Engineering, Defence Institute of Advanced Technology (DU), Ministry of Defence, Girinagar, Pune 411025, Maharashtra, India
- Institute for Frontier Materials, Deakin University, Waurn Ponds Campus, Geelong, Victoria 3216, Australia
| | - Barnali Banerjee
- Department of Chemical Engineering, All India Shree Shivaji Memorial Society's College of Engineering, Pune 411001, Maharashtra, India
| | - Balasubramanian Kandasubramanian
- Nano Surface Texturing, Department of Metallurgical and Materials Engineering, Defence Institute of Advanced Technology (DU), Ministry of Defence, Girinagar, Pune 411025, Maharashtra, India
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Gómez-Farto A, Jiménez-Escobar AL, Pérez-González N, Castán H, Clares B, Arias-Santiago S, Montero-Vílchez T. Development of an Emulgel for the Effective Treatment of Atopic Dermatitis: Biocompatibility and Clinical Investigation. Gels 2024; 10:370. [PMID: 38920917 PMCID: PMC11202525 DOI: 10.3390/gels10060370] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Grants] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 05/01/2024] [Revised: 05/19/2024] [Accepted: 05/21/2024] [Indexed: 06/27/2024] Open
Abstract
Atopic dermatitis (AD) is a common dermatological disease affecting both children and adults. No drug-free emulgel has been developed and studied in vitro and in vivo for the treatment of AD. The aim of this study was to develop and assess the efficacy of a topical emulgel containing hyaluronic acid, glycerol, Calendula officinalis, Aloe vera, polyphenols and EGF for the concomitant treatment in patients with AD aged over 14. Objective skin barrier function parameters were included, such as transepidermal water loss (TEWL), skin temperature, pH, stratum corneum hydration, skin elasticity and erythema. The subjective opinion of the patients was determined including acceptability, absorption, comfort of use and tolerability, as well as the degree of improvement in patients' quality of life. We observed an improvement in the subjective parameters studied and statistically significant differences in the objective parameters. Specifically, we found an improvement in TEWL (p = 0.006), erythema (p = 0.008) and hydration (p < 0.001), parameters indicating an improvement in the epidermal barrier. One hundred per cent of patients were satisfied with the product. Therefore, these results suggest that the product may contribute to the treatment of AD.
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Affiliation(s)
- Almudena Gómez-Farto
- Instituto de Investigación Biotecnológica, Farmacéutica y Medicamentos Huérfanos, S.L, 18016 Granada, Spain; (A.G.-F.); (A.L.J.-E.); (N.P.-G.); (H.C.)
- Department of Pharmacy & Pharmaceutical Technology, Faculty of Pharmacy, University of Granada, 18071 Granada, Spain
| | - Ana Leticia Jiménez-Escobar
- Instituto de Investigación Biotecnológica, Farmacéutica y Medicamentos Huérfanos, S.L, 18016 Granada, Spain; (A.G.-F.); (A.L.J.-E.); (N.P.-G.); (H.C.)
- Department of Pharmacy & Pharmaceutical Technology, Faculty of Pharmacy, University of Granada, 18071 Granada, Spain
| | - Noelia Pérez-González
- Instituto de Investigación Biotecnológica, Farmacéutica y Medicamentos Huérfanos, S.L, 18016 Granada, Spain; (A.G.-F.); (A.L.J.-E.); (N.P.-G.); (H.C.)
- Department of Pharmacy & Pharmaceutical Technology, Faculty of Pharmacy, University of Granada, 18071 Granada, Spain
| | - Herminia Castán
- Instituto de Investigación Biotecnológica, Farmacéutica y Medicamentos Huérfanos, S.L, 18016 Granada, Spain; (A.G.-F.); (A.L.J.-E.); (N.P.-G.); (H.C.)
- Department of Pharmacy & Pharmaceutical Technology, Faculty of Pharmacy, University of Granada, 18071 Granada, Spain
| | - Beatriz Clares
- Department of Pharmacy & Pharmaceutical Technology, Faculty of Pharmacy, University of Granada, 18071 Granada, Spain
- Institute of Nanoscience and Nanotechnology (IN2UB), University of Barcelona, 08028 Barcelona, Spain
- Biosanitary Institute of Granada (ibs.GRANADA), 18012 Granada, Spain; (S.A.-S.); (T.M.-V.)
| | - Salvador Arias-Santiago
- Biosanitary Institute of Granada (ibs.GRANADA), 18012 Granada, Spain; (S.A.-S.); (T.M.-V.)
- Department of Dermatology, Granada School of Medicine, Granada University, Virgen de las Nieves University Hospital, 18012 Granada, Spain
| | - Trinidad Montero-Vílchez
- Biosanitary Institute of Granada (ibs.GRANADA), 18012 Granada, Spain; (S.A.-S.); (T.M.-V.)
- Department of Dermatology, Granada School of Medicine, Granada University, Virgen de las Nieves University Hospital, 18012 Granada, Spain
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Dos Santos Dantas Lima L, Felipe Domingues Passero L, Indriunas A, de Souza Santos I, Francisco Uchôa Coqueiro L, Alexandre Souza da Cruz K, Batista de Almeida A, Carlos Fernandes Galduróz J, Rodrigues E. The Meaning of Plants' Names: A New Discovering Approach to Its Medicinal and/or Toxic Properties. EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE : ECAM 2024; 2024:6678557. [PMID: 38410808 PMCID: PMC10896657 DOI: 10.1155/2024/6678557] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Track Full Text] [Figures] [Subscribe] [Scholar Register] [Received: 04/29/2023] [Revised: 12/22/2023] [Accepted: 02/06/2024] [Indexed: 02/28/2024]
Abstract
Some of the vernacular or scientific names are related to possible medicinal and/or toxic properties that can reveal the presence of potential bioactive agents, contributing to the discovery of new drugs and/or knowledge of the risks associated with their use. This study sought to list the scientific and vernacular names of plants whose lexicons are related to those possible properties of plants and to compare them with the "ethno" (ethnobotanical and ethnopharmacological) and pharmacological data available in the scientific literature. A floating reading of the two classical and reference works on Brazilian medicinal plants was performed, and plants with vernacular and/or scientific names related to the possible medicinal and/or toxic properties were listed. Correlations between the meanings of the species' names (lexicon) and their possible biological properties were made from their translation from Latin by consulting dictionaries. A bibliographic survey was conducted on the "ethno" and pharmacological data for each species. Finally, data from these three dimensions (lexicon, "ethno," and pharmacology) were classified and compared using a bioprospection classification. It resulted in a list of 90 plant species belonging to 47 families. 66 of the 90 species presented "ethno" data from the scientific literature, while 46 species presented pharmacological data. Of these, 46 (69.7%) and 27 (58.7%), respectively, showed equivalence with the possible medicinal and/or toxic properties of plants according to their lexicons. According to this study, half of the plants investigated demonstrate equivalence in the three dimensions analyzed (lexicons, "ethno," and pharmacological data from the scientific literature). Gastrointestinal and nervous system categories are among the most common in all three dimensions. Plant lexicons may be closely linked to the possible medicinal and/or toxic properties and the study of plant lexicons may represent one more approach for the search for new drugs, mainly considering the gastrointestinal, nervous, and parasites categories.
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Affiliation(s)
- Letícia Dos Santos Dantas Lima
- Center for Ethnobotanical and Ethnopharmacological Studies (CEE), Department of Environmental Sciences, Universidade Federal de Sa˜o Paulo (UNIFESP), Rua Prof. Artur Riedel, No 275, Diadema 09972-270, SP, Brazil
| | - Luiz Felipe Domingues Passero
- São Paulo State University (UNESP), Institute of Biosciences, Praça Infante Dom Henrique, s/n, São Vicente 11330-900, SP, Brazil
- Institute for Advanced Studies of Ocean, São Paulo State University (UNESP), São Vicente 11350-011, Brazil
| | - Alexandre Indriunas
- Museu Nacional, Universidade Federal do Rio de Janeiro, Quinta da Boa Vista-São Cristóvão, Rio de Janeiro 20940-040, Brazil
| | - Ingrid de Souza Santos
- Center for Ethnobotanical and Ethnopharmacological Studies (CEE), Department of Environmental Sciences, Universidade Federal de Sa˜o Paulo (UNIFESP), Rua Prof. Artur Riedel, No 275, Diadema 09972-270, SP, Brazil
| | - Luíza Francisco Uchôa Coqueiro
- Center for Ethnobotanical and Ethnopharmacological Studies (CEE), Department of Environmental Sciences, Universidade Federal de Sa˜o Paulo (UNIFESP), Rua Prof. Artur Riedel, No 275, Diadema 09972-270, SP, Brazil
| | - Kayo Alexandre Souza da Cruz
- Center for Ethnobotanical and Ethnopharmacological Studies (CEE), Department of Environmental Sciences, Universidade Federal de Sa˜o Paulo (UNIFESP), Rua Prof. Artur Riedel, No 275, Diadema 09972-270, SP, Brazil
| | - Adriana Batista de Almeida
- Center for Ethnobotanical and Ethnopharmacological Studies (CEE), Department of Environmental Sciences, Universidade Federal de Sa˜o Paulo (UNIFESP), Rua Prof. Artur Riedel, No 275, Diadema 09972-270, SP, Brazil
| | - José Carlos Fernandes Galduróz
- Departament of Psychobiology, Universidade Federal de São Paulo, Rua Botucatu, 862, Edifício Ciências Biomédicas-1° Andar, São Paulo 04724-000, Brazil
| | - Eliana Rodrigues
- Center for Ethnobotanical and Ethnopharmacological Studies (CEE), Department of Environmental Sciences, Universidade Federal de Sa˜o Paulo (UNIFESP), Rua Prof. Artur Riedel, No 275, Diadema 09972-270, SP, Brazil
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Alissa M, Hjazi A, Abusalim GS, Aloraini GS, Alghamdi SA, Alharthi NS, Rizg WY, Hosny KM, Binmadi N. Utilization of nanotechnology and experimental design in the development and optimization of a posaconazole‒calendula oil nanoemulgel for the treatment of mouth disorders. Front Pharmacol 2024; 15:1347551. [PMID: 38434704 PMCID: PMC10905964 DOI: 10.3389/fphar.2024.1347551] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 12/01/2023] [Accepted: 01/31/2024] [Indexed: 03/05/2024] Open
Abstract
Introduction: Essential oil‒based nanoemulsions (NEs) are the subjects of extensive investigation due to their potential to address a variety of oral health issues. NEs are delivery systems that improve lipid medicine solubility and distribution to intended sites. The goal of the current study was to create and enhance a self-nanoemulsifying drug delivery paradigm based on calendula oil (CO) and decorated with chitosan (CS) that could deliver posaconazole (PSZ) for the treatment of gingivitis. Method: Employing a response-surface Box‒Behnken design, PSZ-CO-CS NEs were created with varying amounts of PSZ (10, 15, and 20 mg), percentages of CO (6%, 12%, and 18%), and percentages of CS (0.5%, 1.5%, and 2.5%). Results and conclusion: The optimized formulation resulted in a 22-mm bacterial growth suppression zone, 25-mm fungal growth inhibition zone, droplet sizes of 110 nm, and a viscosity of 750 centipoise (cP). Using the appropriate design, the ideal formulation was produced; it contained 20 mg of PSZ, 18% of CO, and 1.35% of CS. Furthermore, the optimal formulation had a more controlled drug release, larger inhibition zones of bacterial and fungal growth, and desirable rheologic properties. Additionally, the optimized formulation substantially lowered the ulcer index in rats when tested against other formulations. Thus, this investigation showed that PSZ-CO-CS NEs could provide efficient protection against microbially induced gingivitis.
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Affiliation(s)
- Mohammed Alissa
- Department of Medical Laboratory, College of Applied Medical Sciences, Prince Sattam Bin Abdulaziz University, Al-Kharj, Saudi Arabia
| | - Ahmed Hjazi
- Department of Medical Laboratory, College of Applied Medical Sciences, Prince Sattam Bin Abdulaziz University, Al-Kharj, Saudi Arabia
| | - Ghadah S. Abusalim
- Department of Medical Laboratory, College of Applied Medical Sciences, Prince Sattam Bin Abdulaziz University, Al-Kharj, Saudi Arabia
| | - Ghfren S. Aloraini
- Department of Medical Laboratory, College of Applied Medical Sciences, Prince Sattam Bin Abdulaziz University, Al-Kharj, Saudi Arabia
| | - Suad A. Alghamdi
- Department of Medical Laboratory, College of Applied Medical Sciences, Prince Sattam Bin Abdulaziz University, Al-Kharj, Saudi Arabia
| | - Nahed S. Alharthi
- Department of Medical Laboratory, College of Applied Medical Sciences, Prince Sattam Bin Abdulaziz University, Al-Kharj, Saudi Arabia
| | - Waleed Y. Rizg
- Department of Pharmaceutics, Faculty of Pharmacy, King Abdulaziz University, Jeddah, Saudi Arabia
- Center of Innovation in Personalized Medicine (CIPM), 3D Bioprinting Unit, King Abdulaziz University, Jeddah, Saudi Arabia
| | - Khaled M. Hosny
- Department of Pharmaceutics, Faculty of Pharmacy, King Abdulaziz University, Jeddah, Saudi Arabia
- Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmacy, Beni-Suef University, Beni-Suef, Egypt
| | - Nada Binmadi
- Department of Oral Diagnostic Sciences, Faculty of Dentistry, King Abdulaziz University, Jeddah, Saudi Arabia
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11
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Aydin Acar C, Gencer MA, Pehlivanoglu S, Yesilot S, Donmez S. Green and eco-friendly biosynthesis of zinc oxide nanoparticles using Calendula officinalis flower extract: Wound healing potential and antioxidant activity. Int Wound J 2024; 21:e14413. [PMID: 37722846 PMCID: PMC10788648 DOI: 10.1111/iwj.14413] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Grants] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 08/28/2023] [Accepted: 09/10/2023] [Indexed: 09/20/2023] Open
Abstract
This study aimed to produce zinc oxide nanoparticles with Calendula officinalis flower extract (Co-ZnO NPs) using the green synthesis method. In addition, the antioxidant and wound healing potential of synthesized ZnO NPs were evaluated. The absorbance band at 355 nm, which is typical for ZnO NPs, was determined from the UV-Vis absorbance spectrum. The energy-dispersive X-ray spectroscopy (EDS) measurements revealed a high zinc content of 42.90%. The x-ray diffractometer data showed Co-ZnO NPs with an average crystallite size of 17.66 nm. The Co-ZnO NPs did not have apparent cytotoxicity up to 10 μg/mL (IC50 25.96 μg/mL). C. officinalis ZnO NPs showed partial cell migration and percent wound closure (69.1%) compared with control (64.8%). In addition, antioxidant activities of Co-ZnO NPs with 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and 2,2 diphenyl-1 picrylhydrazil (DPPH) were evaluated and radical scavenging activity of 33.49% and 46.63%, respectively, was determined. These results suggest that C. officinalis extract is an effective reducing agent for the green synthesis of ZnO NPs with significant antioxidant and wound healing potential.
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Affiliation(s)
- Cigdem Aydin Acar
- Department of Nursing, Bucak School of HealthBurdur Mehmet Akif Ersoy UniversityBurdurTurkey
- Department of Health and Biomedical SciencesBurdur Mehmet Akif Ersoy UniversityBurdurTurkey
| | | | - Suray Pehlivanoglu
- Department of Molecular Biology and Genetics, Faculty of ScienceNecmettin Erbakan UniversityKonyaTurkey
| | - Sukriye Yesilot
- Department of Nursing, Bucak School of HealthBurdur Mehmet Akif Ersoy UniversityBurdurTurkey
- Department of Health and Biomedical SciencesBurdur Mehmet Akif Ersoy UniversityBurdurTurkey
| | - Soner Donmez
- Bucak School of HealthBurdur Mehmet Akif Ersoy UniversityBurdurTurkey
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Hashemi SS, Pakdin A, Mohammadi A, Keshavarzi A, Mortazavi M, Sanati P. Study the Effect of Calendula officinalis Extract Loaded on Zinc Oxide Nanoparticle Cream in Burn Wound Healing. ACS APPLIED MATERIALS & INTERFACES 2023; 15:59269-59279. [PMID: 38085997 DOI: 10.1021/acsami.3c17350] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 12/28/2023]
Abstract
The skin, the body's largest organ, acts as a protective barrier against pathogens and environmental damage. Skin burns can result from heat, chemicals, friction, or electricity. Nanoscience has recently been utilized to create ointments and creams for burns. Zinc oxide nanoparticles are crucial due to their antimicrobial and antioxidant properties. In this study, a cream containing nanoparticles was loaded with calendula extract, and its ability to promote tissue healing was investigated in Wistar rats with skin burns. The zinc oxide nanoparticles were chemically synthesized and loaded with calendula extract. The morphology and physicochemical properties of the nanoparticles were confirmed by SEM, ZETA size, XRD, and FTIR assays. The MTT technique was employed to assess the cream's impact on fibroblast growth. The antimicrobial activity of the nanoparticles was investigated against Pseudomonas using the MIC method. Real-time PCR was used to determine the expression of the Bax and Bcl-2 genes in aeruginosa. The results showed that zinc oxide nanoparticles at high concentrations increased the proliferation of the fibroblast cells. Histopathological studies showed granulation and epithelialization of the tissue without any hemorrhage or tissue infection during the first days of treatment with this cream. The animal models treated with the cream showed an increase in Bcl-2 gene expression and a decrease in Bax expression. We concluded that zinc oxide nanoparticles loaded with calendula extract have a practical effect in healing burn wounds due to their unique antibacterial properties of zinc oxide nanoparticles and their anti-inflammatory and wound-healing effects. The synergistic effect of these two substances significantly improved the healing process. This newly developed cream can be introduced as a successful and viable treatment option in burn wounds.
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Affiliation(s)
- Seyedeh-Sara Hashemi
- Burn and Wound Healing Research Center, Shiraz University of Medical Sciences, Shiraz 71348-14336, Iran
| | - Amir Pakdin
- Division of Plastic and Reconstructive Surgery, Department of Surgery, Shiraz University of Medical Sciences, Shiraz 71348-14336, Iran
| | - Aliakbar Mohammadi
- Burn and Wound Healing Research Center, Shiraz University of Medical Sciences, Shiraz 71348-14336, Iran
- Division of Plastic and Reconstructive Surgery, Department of Surgery, Shiraz University of Medical Sciences, Shiraz 71348-14336, Iran
| | - Abdolkhalegh Keshavarzi
- Burn and Wound Healing Research Center, Shiraz University of Medical Sciences, Shiraz 71348-14336, Iran
| | - Mojtaba Mortazavi
- Department of Biotechnology, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman 76318-85356, Iran
| | - Parisa Sanati
- Burn and Wound Healing Research Center, Shiraz University of Medical Sciences, Shiraz 71348-14336, Iran
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Mohanta YK, Mishra AK, Nongbet A, Chakrabartty I, Mahanta S, Sarma B, Panda J, Panda SK. Potential use of the Asteraceae family as a cure for diabetes: A review of ethnopharmacology to modern day drug and nutraceuticals developments. Front Pharmacol 2023; 14:1153600. [PMID: 37608892 PMCID: PMC10441548 DOI: 10.3389/fphar.2023.1153600] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.5] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 01/29/2023] [Accepted: 06/29/2023] [Indexed: 08/24/2023] Open
Abstract
The diabetes-associated mortality rate is increasing annually, along with the severity of its accompanying disorders that impair human health. Worldwide, several medicinal plants are frequently urged for the management of diabetes. Reports are available on the use of medicinal plants by traditional healers for their blood-sugar-lowering effects, along with scientific evidence to support such claims. The Asteraceae family is one of the most diverse flowering plants, with about 1,690 genera and 32,000 species. Since ancient times, people have consumed various herbs of the Asteraceae family as food and employed them as medicine. Despite the wide variety of members within the family, most of them are rich in naturally occurring polysaccharides that possess potent prebiotic effects, which trigger their use as potential nutraceuticals. This review provides detailed information on the reported Asteraceae plants traditionally used as antidiabetic agents, with a major focus on the plants of this family that are known to exert antioxidant, hepatoprotective, vasodilation, and wound healing effects, which further action for the prevention of major diseases like cardiovascular disease (CVD), liver cirrhosis, and diabetes mellitus (DM). Moreover, this review highlights the potential of Asteraceae plants to counteract diabetic conditions when used as food and nutraceuticals. The information documented in this review article can serve as a pioneer for developing research initiatives directed at the exploration of Asteraceae and, at the forefront, the development of a botanical drug for the treatment of DM.
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Affiliation(s)
- Yugal Kishore Mohanta
- Nano-biotechnology and Translational Knowledge Laboratory, Department of Applied Biology, School of Biological Sciences, University of Science and Technology Meghalaya (USTM), Techno City, Meghalaya, India
- Centre for Herbal Pharmacology and Environmental Sustainability, Chettinad Hospital and Research Institute, Chettinad Academy of Research and Education, kelambakkam, Tamil Nadu, India
| | | | - Amilia Nongbet
- Department of Botany, School of Biological Sciences, University of Science and Technology Meghalaya (USTM), Techno City, Meghalaya, India
| | - Ishani Chakrabartty
- Learning and Development Solutions, Indegene Pvt. Ltd., Manyata Tech Park, Bangalore, India
| | - Saurov Mahanta
- Guwahati Centre, National Institute of Electronics and Information Technology (NIELIT), Guwahati, Assam, India
| | - Bhaskar Sarma
- Department of Botany, Dhemaji College, Dhemaji, Assam, India
| | - Jibanjyoti Panda
- Nano-biotechnology and Translational Knowledge Laboratory, Department of Applied Biology, School of Biological Sciences, University of Science and Technology Meghalaya (USTM), Techno City, Meghalaya, India
| | - Sujogya Kumar Panda
- Center of Environment Climate Change and Public Health, RUSA 2.0, Utkal University, Bhubaneswar, Odisha, India
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Ungureanu AR, Chițescu CL, Luță EA, Moroșan A, Mihaiescu DE, Mihai DP, Costea L, Ozon EA, Fița AC, Balaci TD, Boscencu R, Gîrd CE. Outlook on Chronic Venous Disease Treatment: Phytochemical Screening, In Vitro Antioxidant Activity and In Silico Studies for Three Vegetal Extracts. Molecules 2023; 28:molecules28093668. [PMID: 37175078 PMCID: PMC10180301 DOI: 10.3390/molecules28093668] [Citation(s) in RCA: 7] [Impact Index Per Article: 3.5] [Reference Citation Analysis] [Abstract] [Key Words] [Grants] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 03/31/2023] [Revised: 04/20/2023] [Accepted: 04/21/2023] [Indexed: 05/15/2023] Open
Abstract
Chronic venous disease is one of the most common vascular diseases; the signs and symptoms are varied and are often neglected in the early stages. Vascular damage is based on proinflammatory, prothrombotic, prooxidant activity and increased expression of several matrix metalloproteinases (MMPs). The aim of this research is preparation and preliminary characterization of three vegetal extracts (Sophorae flos-SE, Ginkgo bilobae folium-GE and Calendulae flos-CE). The obtained dry extracts were subjected to phytochemical screening (FT-ICR-MS, UHPLC-HRMS/MS) and quantitative analysis (UHPLC-HRMS/MS, spectrophotometric methods). Antioxidant activity was evaluated using three methods: FRAP, DPPH and ABTS. More than 30 compounds were found in each extract. The amount of flavones follows the succession: SE > GE > CE; the amount of phenolcarboxylic acids follows: SE > CE > GE; and the amount of polyphenols follows: SE > GE > CE. Results for FRAP method varied as follows: SE > CE > GE; results for the DPPH method followed: SE > GE > CE; and results for ABTS followed: SE > GE > CE. Strong and very strong correlations (appreciated by Pearson coefficient) have been observed between antioxidant activity and the chemical content of extracts. Molecular docking studies revealed the potential of several identified phytochemicals to inhibit the activity of four MMP isoforms. In conclusion, these three extracts have potential in the treatment of chronic venous disease, based on their phytochemical composition.
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Affiliation(s)
- Andreea Roxana Ungureanu
- Faculty of Pharmacy, Carol Davila University of Medicine and Pharmacy, Traian Vuia 6, 020956 Bucharest, Romania
| | - Carmen Lidia Chițescu
- Faculty of Medicine and Pharmacy, Dunărea de Jos University of Galați, A.I. Cuza 35, 800010 Galați, Romania
| | - Emanuela Alice Luță
- Faculty of Pharmacy, Carol Davila University of Medicine and Pharmacy, Traian Vuia 6, 020956 Bucharest, Romania
| | - Alina Moroșan
- Faculty of Chemical Engineering and Biotechnologies, University of Politehnica, Gheorghe Polizu 1-7, 011061 Bucharest, Romania
| | - Dan Eduard Mihaiescu
- Faculty of Chemical Engineering and Biotechnologies, University of Politehnica, Gheorghe Polizu 1-7, 011061 Bucharest, Romania
| | - Dragoș Paul Mihai
- Faculty of Pharmacy, Carol Davila University of Medicine and Pharmacy, Traian Vuia 6, 020956 Bucharest, Romania
| | - Liliana Costea
- Faculty of Pharmacy, Carol Davila University of Medicine and Pharmacy, Traian Vuia 6, 020956 Bucharest, Romania
| | - Emma Adriana Ozon
- Faculty of Pharmacy, Carol Davila University of Medicine and Pharmacy, Traian Vuia 6, 020956 Bucharest, Romania
| | - Ancuța Cătălina Fița
- Faculty of Pharmacy, Carol Davila University of Medicine and Pharmacy, Traian Vuia 6, 020956 Bucharest, Romania
| | - Teodora Dalila Balaci
- Faculty of Pharmacy, Carol Davila University of Medicine and Pharmacy, Traian Vuia 6, 020956 Bucharest, Romania
| | - Rica Boscencu
- Faculty of Pharmacy, Carol Davila University of Medicine and Pharmacy, Traian Vuia 6, 020956 Bucharest, Romania
| | - Cerasela Elena Gîrd
- Faculty of Pharmacy, Carol Davila University of Medicine and Pharmacy, Traian Vuia 6, 020956 Bucharest, Romania
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15
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Radovanović K, Gavarić N, Aćimović M. Anti-Inflammatory Properties of Plants from Serbian Traditional Medicine. Life (Basel) 2023; 13:life13040874. [PMID: 37109403 PMCID: PMC10146037 DOI: 10.3390/life13040874] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 02/20/2023] [Revised: 03/15/2023] [Accepted: 03/24/2023] [Indexed: 03/29/2023] Open
Abstract
Inflammation is a natural protective response of the human body to a variety of hostile agents and noxious stimuli. Standard anti-inflammatory therapy includes drugs whose usage is associated with a number of side effects. Since ancient times, natural compounds have been used for the treatment of inflammation. Traditionally, the use of medicinal plants is considered safe, inexpensive, and widely acceptable. In Serbia, traditional medicine, based on the strong belief in the power of medicinal herbs, is the widespread form of treatment. This is supported by the fact that Serbia is classified as one of 158 world centers of biodiversity, which confirms that this country is a treasure of medicinal herbs. Some of the most used herbs for the treatment of inflammations of various causes in Serbian tradition are yarrow, common agrimony, couch grass, onion, garlic, marshmallow, common birch, calendula, liquorice, walnut, St. John’s wort, chamomile, peppermint, white willow, sage, and many others. The biological activity and anti-inflammatory effect of selected plants are attributed to different groups of secondary biomolecules such as flavonoids, phenolic acids, sterols, terpenoids, sesquiterpenes, and tannins. This paper provides an overview of plants with traditional anti-inflammatory use in Serbia with reference to available studies that examined this effect. Plants used in traditional medicine could be a powerful source for the development of new remedies. Therefore intensive research on the bioactive potential of medicinal plants in each region should be the focus of scientists around the world.
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Centella asiatica and Its Metabolite Asiatic Acid: Wound Healing Effects and Therapeutic Potential. Metabolites 2023; 13:metabo13020276. [PMID: 36837896 PMCID: PMC9966672 DOI: 10.3390/metabo13020276] [Citation(s) in RCA: 43] [Impact Index Per Article: 21.5] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 01/18/2023] [Revised: 01/28/2023] [Accepted: 02/02/2023] [Indexed: 02/17/2023] Open
Abstract
An intense effort has been focused on new therapeutic approaches and the development of technologies for more efficient and rapid wound healing. The research for plants used for long time in traditional medicine in the treatment of wound has become a promising strategy to obtain drugs therapeutically useful in the acute and chronic wound management. In this context, Centella asiatica (Apiaceae) has been used to treat a variety of skin diseases, such as leprosy, lupus, varicose ulcers, eczema and psoriasis, in Asiatic traditional medicine for thousands of years. Studies have shown that Centella asiatica extracts (CAE) display activity in tissue regeneration, cell migration and wound repair process by promoting fibroblast proliferation and collagen synthesis. Preliminary findings have shown that the asiatic acid is one of the main active constituents of C. asiatica, directly associated with its healing activity. Thus, this study discusses aspects of the effects of Centella asiatica and its active component, asiatic acid, in different stages of the healing process of cutaneous wounds, including phytochemical and antimicrobial aspects that contribute to its therapeutic potential.
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Melnyk N, Vlasova I, Skowrońska W, Bazylko A, Piwowarski JP, Granica S. Current Knowledge on Interactions of Plant Materials Traditionally Used in Skin Diseases in Poland and Ukraine with Human Skin Microbiota. Int J Mol Sci 2022; 23:ijms23179644. [PMID: 36077043 PMCID: PMC9455764 DOI: 10.3390/ijms23179644] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 07/25/2022] [Revised: 08/19/2022] [Accepted: 08/21/2022] [Indexed: 11/16/2022] Open
Abstract
Skin disorders of different etiology, such as dermatitis, atopic dermatitis, eczema, psoriasis, wounds, burns, and others, are widely spread in the population. In severe cases, they require the topical application of drugs, such as antibiotics, steroids, and calcineurin inhibitors. With milder symptoms, which do not require acute pharmacological interventions, medications, dietary supplements, and cosmetic products of plant material origin are gaining greater popularity among professionals and patients. They are applied in various pharmaceutical forms, such as raw infusions, tinctures, creams, and ointments. Although plant-based formulations have been used by humankind since ancient times, it is often unclear what the mechanisms of the observed beneficial effects are. Recent advances in the contribution of the skin microbiota in maintaining skin homeostasis can shed new light on understanding the activity of topically applied plant-based products. Although the influence of various plants on skin-related ailments are well documented in vivo and in vitro, little is known about the interaction with the network of the skin microbial ecosystem. The review aims to summarize the hitherto scientific data on plant-based topical preparations used in Poland and Ukraine and indicate future directions of the studies respecting recent developments in understanding the etiology of skin diseases. The current knowledge on investigations of interactions of plant materials/extracts with skin microbiome was reviewed for the first time.
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Affiliation(s)
- Natalia Melnyk
- Microbiota Lab, Department of Pharmacognosy and Molecular Basis of Phytotherapy, Medical University of Warsaw, Banacha 1, 02-097 Warsaw, Poland
| | - Inna Vlasova
- Microbiota Lab, Department of Pharmacognosy and Molecular Basis of Phytotherapy, Medical University of Warsaw, Banacha 1, 02-097 Warsaw, Poland
- Department of Pharmacognosy, National University of Pharmacy, 53 Pushkinska Str., 61002 Kharkiv, Ukraine
| | - Weronika Skowrońska
- Department of Pharmacognosy and Molecular Basis of Phytotherapy, Medical University of Warsaw, Banacha 1, 02-097 Warsaw, Poland
| | - Agnieszka Bazylko
- Department of Pharmacognosy and Molecular Basis of Phytotherapy, Medical University of Warsaw, Banacha 1, 02-097 Warsaw, Poland
| | - Jakub P. Piwowarski
- Microbiota Lab, Department of Pharmacognosy and Molecular Basis of Phytotherapy, Medical University of Warsaw, Banacha 1, 02-097 Warsaw, Poland
| | - Sebastian Granica
- Microbiota Lab, Department of Pharmacognosy and Molecular Basis of Phytotherapy, Medical University of Warsaw, Banacha 1, 02-097 Warsaw, Poland
- Correspondence: ; Tel.: +48-225-720-9053
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A Comprehensive Review of Natural Compounds for Wound Healing: Targeting Bioactivity Perspective. Int J Mol Sci 2022; 23:ijms23179573. [PMID: 36076971 PMCID: PMC9455684 DOI: 10.3390/ijms23179573] [Citation(s) in RCA: 25] [Impact Index Per Article: 8.3] [Reference Citation Analysis] [Abstract] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 08/05/2022] [Revised: 08/20/2022] [Accepted: 08/20/2022] [Indexed: 11/17/2022] Open
Abstract
Wound healing is a recovering process of damaged tissues by replacing dysfunctional injured cellular structures. Natural compounds for wound treatment have been widely used for centuries. Numerous published works provided reviews of natural compounds for wound healing applications, which separated the approaches based on different categories such as characteristics, bioactivities, and modes of action. However, current studies provide reviews of natural compounds that originated from only plants or animals. In this work, we provide a comprehensive review of natural compounds sourced from both plants and animals that target the different bioactivities of healing to promote wound resolution. The compounds were classified into four main groups (i.e., anti-inflammation, anti-oxidant, anti-bacterial, and collagen promotion), mostly studied in current literature from 1992 to 2022. Those compounds are listed in tables for readers to search for their origin, bioactivity, and targeting phases in wound healing. We also reviewed the trend in using natural compounds for wound healing.
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Lukhey MS, Shende P. Advancement in wound healing treatment using functional nanocarriers. INT J POLYM MATER PO 2022. [DOI: 10.1080/00914037.2022.2099393] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 10/17/2022]
Affiliation(s)
- Mihir S. Lukhey
- Shobhaben Pratapbhai Patel School of Pharmacy and Technology Management, SVKM’S NMIMS, Mumbai, India
| | - Pravin Shende
- Shobhaben Pratapbhai Patel School of Pharmacy and Technology Management, SVKM’S NMIMS, Mumbai, India
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The Effect of Hydroalcoholic Calendula Officinalis Extract on Androgen-Induced Polycystic Ovary Syndrome Model in Female Rat. BIOMED RESEARCH INTERNATIONAL 2022; 2022:7402598. [PMID: 35845946 PMCID: PMC9283045 DOI: 10.1155/2022/7402598] [Citation(s) in RCA: 6] [Impact Index Per Article: 2.0] [Reference Citation Analysis] [Abstract] [Track Full Text] [Download PDF] [Figures] [Subscribe] [Scholar Register] [Received: 01/29/2022] [Revised: 05/12/2022] [Accepted: 06/04/2022] [Indexed: 11/17/2022]
Abstract
Background. Polycystic ovary syndrome (PCOS) is the most common hormonal disorder in women of reproductive age, and the major cause of infertility. Today, using medicinal plants instead of chemical drugs could be an alternative treatment option for PCOS. The purpose of this study was to determine the effect of Calendula officinalis hydroalcoholic extract on PCOS in rats. Method. 60 female adult rats were randomly divided into six groups, including control, sham, PCOS group, and treated PCOS groups receiving hydroalcoholic extract of Calendula officinalis with different dosages of 200, 500, and 1000 mg/kg. PCOS was induced by subcutaneous injection of DHEA 6 mg/100 g bw for 35 days. For two weeks, the extract was taken orally. The serum glucose, insulin, sex hormone levels, and oxidative status were measured at the end of the experiment. The ovaries were dissected for histomorphometric and pathological analysis. Results. When compared to the control and sham groups, the PCOS group showed a significant increase in glucose, insulin, testosterone, and malondialdehyde (MDA) concentrations, cystic and atretic follicles, and thickness of the theca and tunica albuginea layers, and a significant decrease in LH concentration, total antioxidant capacity, corpus luteum, antral follicles, and oocyte diameter. The mean concentration of FSH, on the other hand, did not change significantly. A trend of improvement was found in the treated groups with high doses of Calendula officinalis extract. Conclusion. In rats with PCOS and nonovulation, Calendula officinalis hydroalcoholic extract improved oxidative stress, restored folliculogenesis, and increased ovulation.
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Vitale S, Colanero S, Placidi M, Di Emidio G, Tatone C, Amicarelli F, D’Alessandro AM. Phytochemistry and Biological Activity of Medicinal Plants in Wound Healing: An Overview of Current Research. MOLECULES (BASEL, SWITZERLAND) 2022; 27:molecules27113566. [PMID: 35684503 PMCID: PMC9182061 DOI: 10.3390/molecules27113566] [Citation(s) in RCA: 31] [Impact Index Per Article: 10.3] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Download PDF] [Figures] [Subscribe] [Scholar Register] [Received: 03/31/2022] [Revised: 05/28/2022] [Accepted: 05/30/2022] [Indexed: 12/12/2022]
Abstract
Wound healing is a complicated process, and the effective management of wounds is a major challenge. Natural herbal remedies have now become fundamental for the management of skin disorders and the treatment of skin infections due to the side effects of modern medicine and lower price for herbal products. The aim of the present study is to summarize the most recent in vitro, in vivo, and clinical studies on major herbal preparations, their phytochemical constituents, and new formulations for wound management. Research reveals that several herbal medicaments have marked activity in the management of wounds and that this activity is ascribed to flavonoids, alkaloids, saponins, and phenolic compounds. These phytochemicals can act at different stages of the process by means of various mechanisms, including anti-inflammatory, antimicrobial, antioxidant, collagen synthesis stimulating, cell proliferation, and angiogenic effects. The application of natural compounds using nanotechnology systems may provide significant improvement in the efficacy of wound treatments. Increasing the clinical use of these therapies would require safety assessment in clinical trials.
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Affiliation(s)
- Stefania Vitale
- Department of Life, Health and Environmental Sciences, University of L’Aquila, 67100 L’Aquila, Italy; (S.V.); (M.P.); (G.D.E.); (C.T.); (F.A.)
| | - Sara Colanero
- Department of Biosciences, University of Milan, Via Giovanni Celoria 26, 20133 Milan, Italy;
| | - Martina Placidi
- Department of Life, Health and Environmental Sciences, University of L’Aquila, 67100 L’Aquila, Italy; (S.V.); (M.P.); (G.D.E.); (C.T.); (F.A.)
| | - Giovanna Di Emidio
- Department of Life, Health and Environmental Sciences, University of L’Aquila, 67100 L’Aquila, Italy; (S.V.); (M.P.); (G.D.E.); (C.T.); (F.A.)
| | - Carla Tatone
- Department of Life, Health and Environmental Sciences, University of L’Aquila, 67100 L’Aquila, Italy; (S.V.); (M.P.); (G.D.E.); (C.T.); (F.A.)
| | - Fernanda Amicarelli
- Department of Life, Health and Environmental Sciences, University of L’Aquila, 67100 L’Aquila, Italy; (S.V.); (M.P.); (G.D.E.); (C.T.); (F.A.)
| | - Anna Maria D’Alessandro
- Department of Life, Health and Environmental Sciences, University of L’Aquila, 67100 L’Aquila, Italy; (S.V.); (M.P.); (G.D.E.); (C.T.); (F.A.)
- Correspondence:
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22
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El-Aassar MR, Ibrahim OM, Al-Oanzi ZH. Biotechnological Applications of Polymeric Nanofiber Platforms Loaded with Diverse Bioactive Materials. Polymers (Basel) 2021; 13:3734. [PMID: 34771291 PMCID: PMC8586957 DOI: 10.3390/polym13213734] [Citation(s) in RCA: 16] [Impact Index Per Article: 4.0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Journal Information] [Subscribe] [Scholar Register] [Received: 10/10/2021] [Revised: 10/23/2021] [Accepted: 10/24/2021] [Indexed: 02/07/2023] Open
Abstract
This review article highlights the critical research and formative works relating to nanofiber composites loaded with bioactive materials for diverse applications, and discusses the recent research on the use of electrospun nanofiber incorporating bioactive compounds such as essential oils, herbal bioactive components, plant extracts, and metallic nanoparticles. Inevitably, with the common advantages of bioactive components and polymer nanofibers, electrospun nanofibers containing bioactive components have attracted intense interests for their applications in biomedicine and cancer treatment. Many studies have only concentrated on the production and performance of electrospun nanofiber loaded with bioactive components; in this regard, the features of different types of electrospun nanofiber incorporating a wide variety of bioactive compounds and their developing trends are summarized and assessed in the present article, as is the feasible use of nanofiber technology to produce products on an industrial scale in different applications.
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Affiliation(s)
- M. R. El-Aassar
- Department of Chemistry, College of Science, Jouf University, Sakaka 75471, Saudi Arabia
- Polymer Materials Research Department, Advanced Technology and New Material Institute, City of Scientific Research and Technological Applications (SRTA-City), New Borg El-Arab City, Alexandria 21934, Egypt
| | - Omar M. Ibrahim
- Department of Medicine, Division of Oncology, Washington University School of Medicine, St. Louis, MO 63110, USA;
| | - Ziad H. Al-Oanzi
- Department of Clinical Laboratories Sciences, College of Applied Medical Sciences, Jouf University, Sakaka 75471, Saudi Arabia
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Wu XX, Siu WS, Wat CL, Chan CL, Koon CM, Li X, Cheng W, Ma H, Tsang MSM, Lam CWK, Leung PC, Lau CBS, Wong CK. Effects of topical application of a tri-herb formula on inflammatory dry-skin condition in mice with oxazolone-induced atopic dermatitis. PHYTOMEDICINE : INTERNATIONAL JOURNAL OF PHYTOTHERAPY AND PHYTOPHARMACOLOGY 2021; 91:153691. [PMID: 34425472 DOI: 10.1016/j.phymed.2021.153691] [Citation(s) in RCA: 4] [Impact Index Per Article: 1.0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 01/19/2021] [Revised: 06/21/2021] [Accepted: 07/28/2021] [Indexed: 06/13/2023]
Abstract
BACKGROUND Atopic dermatitis (AD) is a chronic relapsing inflammatory and pruritic skin disease, affecting 10-20% of the population worldwide. Paeonia suffruticosa Andrews (Paeoniaceae) (Cortex Moutan) and Mentha haplocalyx Briq. (Labiatae) (Herba Menthae) have shown beneficial effects on AD. Calendula officinalis L. (Asteraceae) is commonly used for treating skin rashes and wounds. PURPOSE In the present study, a three-herbs formula including Cortex Moutan and Herba Menthae, together with C. officinalis at 1:1:1 weight ratio was used as a topical agent and its therapeutic effects on AD was investigated. METHODS In vitro effects of individual herbs and three-herbs formula (0.125-1 mg/ml) were examined using cytokine release assay on human mast HMC-1 cells, inflammation test on murine macrophage RAW cells and human keratinocyte (HaCaT) cells, and migration scratch assay on human umbilical vein endothelial cells (HUVEC). The contributing functional pathway of three-herbs formula in AD was explored using Western Blot assay in HMC-1 cells. Oxazolone-induced AD-like mice model was also used to investigate the in vivo therapeutic effect of the topical application of the three-herbs formula. RESULTS Herba Menthae, Cortex Moutan, and three-herbs formula significantly reduced the production of IL-6 and tumor necrosis factor (TNF)-α in HMC-1 cells, inhibited the expression of IL-6, IL-8 and CCL2 in TNF-α/IFN-γ stimulated HaCaT cells, and suppressed the lipopolysaccharide (LPS)-induced nitric oxide (NO) production in RAW 264.7 cells. Moreover, Herba Menthae and three-herbs formula significantly suppressed CCL2 and TNF-α production in LPS-induced RAW 264.7 cells. C. officinalis and three-herbs formula promoted wound healing in HUVEC. For intracellular mechanisms, three-herbs formula inhibited the expressions of molecules in STAT1 and STAT3-dependent pathways. In vivo model showed that topical application of three-herbs formula on challenged ear reduced ear swelling and mice scratching frequencies. H&E and toluidine blue staining of the challenged ear tissue demonstrated that three-herbs formula reduced the epidermal thickness and mast cell infiltration, respectively. CONCLUSION The three-herbs formula of Cortex Moutan, Herba Menthae and C. officinalis at 1:1:1 (w/w) exhibited anti-inflammatory effect and promotion of cell migration in vitro. It also alleviated ear redness, swelling, epidermal thickness and inflammation of the OXA-induced AD mice. These findings suggest a potential beneficial role of the topical application of the three-herbs formula for treatment of AD.
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Affiliation(s)
- Xiao Xiao Wu
- Institute of Chinese Medicine, The Chinese University of Hong Kong, Hong Kong, China; State Key Laboratory of Research on Bioactivities and Clinical Applications of Medicinal Plants (CUHK), The Chinese University of Hong Kong, Hong Kong, China
| | - Wing Sum Siu
- Institute of Chinese Medicine, The Chinese University of Hong Kong, Hong Kong, China; State Key Laboratory of Research on Bioactivities and Clinical Applications of Medicinal Plants (CUHK), The Chinese University of Hong Kong, Hong Kong, China
| | - Chi Ling Wat
- Institute of Chinese Medicine, The Chinese University of Hong Kong, Hong Kong, China; State Key Laboratory of Research on Bioactivities and Clinical Applications of Medicinal Plants (CUHK), The Chinese University of Hong Kong, Hong Kong, China
| | - Chung Lap Chan
- Institute of Chinese Medicine, The Chinese University of Hong Kong, Hong Kong, China; State Key Laboratory of Research on Bioactivities and Clinical Applications of Medicinal Plants (CUHK), The Chinese University of Hong Kong, Hong Kong, China
| | - Chi Man Koon
- Institute of Chinese Medicine, The Chinese University of Hong Kong, Hong Kong, China; State Key Laboratory of Research on Bioactivities and Clinical Applications of Medicinal Plants (CUHK), The Chinese University of Hong Kong, Hong Kong, China
| | - Xiang Li
- Institute of Chinese Medicine, The Chinese University of Hong Kong, Hong Kong, China; State Key Laboratory of Research on Bioactivities and Clinical Applications of Medicinal Plants (CUHK), The Chinese University of Hong Kong, Hong Kong, China
| | - Wen Cheng
- Institute of Chinese Medicine, The Chinese University of Hong Kong, Hong Kong, China; State Key Laboratory of Research on Bioactivities and Clinical Applications of Medicinal Plants (CUHK), The Chinese University of Hong Kong, Hong Kong, China
| | - Hui Ma
- Institute of Chinese Medicine, The Chinese University of Hong Kong, Hong Kong, China; State Key Laboratory of Research on Bioactivities and Clinical Applications of Medicinal Plants (CUHK), The Chinese University of Hong Kong, Hong Kong, China
| | - Miranda Sin Man Tsang
- Institute of Chinese Medicine, The Chinese University of Hong Kong, Hong Kong, China; State Key Laboratory of Research on Bioactivities and Clinical Applications of Medicinal Plants (CUHK), The Chinese University of Hong Kong, Hong Kong, China; Department of Chemical Pathology, Prince of Wales Hospital, The Chinese University of Hong Kong, Hong Kong, China
| | - Christopher Wai-Kei Lam
- Faculty of Medicine and State Key Laboratory of Quality Research in Chinese Medicines, Macau University of Science and Technology, Macau, China
| | - Ping Chung Leung
- Institute of Chinese Medicine, The Chinese University of Hong Kong, Hong Kong, China; State Key Laboratory of Research on Bioactivities and Clinical Applications of Medicinal Plants (CUHK), The Chinese University of Hong Kong, Hong Kong, China
| | - Clara Bik San Lau
- Institute of Chinese Medicine, The Chinese University of Hong Kong, Hong Kong, China; State Key Laboratory of Research on Bioactivities and Clinical Applications of Medicinal Plants (CUHK), The Chinese University of Hong Kong, Hong Kong, China
| | - Chun-Kwok Wong
- Institute of Chinese Medicine, The Chinese University of Hong Kong, Hong Kong, China; State Key Laboratory of Research on Bioactivities and Clinical Applications of Medicinal Plants (CUHK), The Chinese University of Hong Kong, Hong Kong, China; Department of Chemical Pathology, Prince of Wales Hospital, The Chinese University of Hong Kong, Hong Kong, China; Li Dak Sum Yip Yio Chin R & D Centre for Chinese Medicine, The Chinese University of Hong Kong, Hong Kong, China.
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Yazarlu O, Iranshahi M, Kashani HRK, Reshadat S, Habtemariam S, Iranshahy M, Hasanpour M. Perspective on the application of medicinal plants and natural products in wound healing: A mechanistic review. Pharmacol Res 2021; 174:105841. [PMID: 34419563 DOI: 10.1016/j.phrs.2021.105841] [Citation(s) in RCA: 88] [Impact Index Per Article: 22.0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Received: 06/08/2021] [Revised: 08/14/2021] [Accepted: 08/17/2021] [Indexed: 12/14/2022]
Abstract
Wound is defined as any injury to the body such as damage to the epidermis of the skin and disturbance to its normal anatomy and function. Since ancient times, the importance of wound healing has been recognized, and many efforts have been made to develop novel wound dressings made of the best material for rapid and effective wound healing. Medicinal plants play a great role in the wound healing process. In recent decades, many studies have focused on the development of novel wound dressings that incorporate medicinal plant extracts or their purified active compounds, which are potential alternatives to conventional wound dressings. Several studies have also investigated the mechanism of action of various herbal medicines in wound healing process. This paper attempts to highlight and review the mechanistic perspective of wound healing mediated by plant-based natural products. The findings showed that herbal medicines act through multiple mechanisms and are involved in various stages of wound healing. Some herbal medicines increase the expression of vascular endothelial growth factor (VEGF) and transforming growth factor-β (TGF-β) which play important role in stimulation of re-epithelialization, angiogenesis, formation of granulation tissue, and collagen fiber deposition. Some other wound dressing containing herbal medicines act as inhibitor of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and inducible nitric oxide synthase (iNOS) protein expression thereby inducing antioxidant and anti-inflammatory properties in various phases of the wound healing process. Besides the growing public interest in traditional and alternative medicine, the use of herbal medicine and natural products for wound healing has many advantages over conventional medicines, including greater effectiveness due to diverse mechanisms of action, antibacterial activity, and safety in long-term wound dressing usage.
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Affiliation(s)
- Omid Yazarlu
- Mashhad University of Medical Sciences, Department of General Surgery, Mashhad, Iran
| | - Mehrdad Iranshahi
- Biotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran
| | | | - Sara Reshadat
- Department of Internal Medicine, Semnan University of Medical Sciences, Semnan, Iran
| | - Solomon Habtemariam
- Pharmacognosy Research Laboratories and Herbal Analysis Services UK, University of Greenwich, Central Avenue, Chatham-Maritime, Kent ME4 4TB, UK
| | - Milad Iranshahy
- Department of Pharmacognosy, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.
| | - Maede Hasanpour
- Biotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran.
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Niazi A, Moradi M, Askari VR, Sharifi N. Effect of Complementary Medicine on Pain Relief and Wound Healing after Cesarean Section: A Systematic Review. J Pharmacopuncture 2021; 24:41-53. [PMID: 34249394 PMCID: PMC8220509 DOI: 10.3831/kpi.2021.24.2.41] [Citation(s) in RCA: 6] [Impact Index Per Article: 1.5] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 03/01/2020] [Revised: 04/28/2021] [Accepted: 05/18/2021] [Indexed: 11/18/2022] Open
Abstract
Objectives Cesarean sections are one of the common surgical procedures around the world. Management of cesarean section side effects, including pain, hematoma, delayed wound healing, is of particular importance in maintaining maternal health and ability to care for the baby. The tendency to use complementary medicine strategies is on the rise because of the easy treatment with low side effects. The purpose of this study was to systematically review the efficacy and safety of clinical trials performed in Iran and worldwide on the effect of complementary medicine on pain relief and wound healing after cesarean section. Methods PRISMA checklist was followed to prepare the report of this systematic review. The search process was carried out on databases on databases of Magiran, SID, Iran Medex, Scopus, Pub Med, Science direct, Medline and Cochrane library using keywords of cesarean, pain, wound healing, Herbal medicine, acupressure, massage, complementary medicine and their Persian equivalent and all possible combinations, from inception until February 2020. We used the Jadad scale to assess the quality of the searched articles. According to the Jadad scale, the articles with a score of at least 3 were included in the study. Results Finally, 28 clinical trials (with a sample size of 3,245) scored at least 3 on the Jadad scale were included into the analysis. This article reviewed 13 articles on medicinal herbs, 4 articles on massage, 1 article on reflexology, 2 articles on acupressure. Conclusion According to the present review, the use of medicinal herbs was the most common method of complementary medicine in pain relief and wound healing after cesarean section.
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Affiliation(s)
- Azin Niazi
- School of Nursing and Midwifery, Mashhad University of Medical Sciences, Mashhad, Iran
| | - Maryam Moradi
- Nursing and Midwifery Care Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
| | - Vahid Reza Askari
- Pharmacological Research Center of Medicinal Plants, Mashhad University of Medical Sciences, Mashhad, Iran
| | - Neda Sharifi
- Department of Midwifery, Mashhad Branch, Islamic Azad University, Mashhad, Iran
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Agatonovic-Kustrin S, Ramenskaya G, Kustrin E, Morton DW. Characterisation of α-amylase inhibitors in marigold plants via bioassay-guided high-performance thin-layer chromatography and attenuated total reflectance-Fourier transform infrared spectroscopy. J Chromatogr B Analyt Technol Biomed Life Sci 2021; 1173:122676. [PMID: 33848802 DOI: 10.1016/j.jchromb.2021.122676] [Citation(s) in RCA: 5] [Impact Index Per Article: 1.3] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 01/31/2021] [Revised: 03/17/2021] [Accepted: 03/19/2021] [Indexed: 12/14/2022]
Abstract
A high-performance thin-layer chromatography with microchemical derivatization and bioassay guided detection was used for bioanalytical profiling of selected marigold plant extracts. Anisaldehyde/sulfuric acid reagent and thymol/sulfuric acid reagent were used to visualize separated components on the chromatograms. Antioxidant activity and α-amylase inhibition were assessed with 2 bioassays, DPPH assay to detect free radical scavengers and starch-iodineassay method to detect compounds that inhibit α-amylase. The highest antioxidant activity of 10.12 μg of gallic acid equivalents (GAE) per 20 µL of extract was measured in extract from Tagetes flowers and the lowest in the extract from Calendula leaves with 5.10 μg of GAE. Multiple zones of α-amylase inhibition were detected. A detailed analysis of the ATR-FTIR spectra from the bands at RF = 0.24 suggest that faradiol esters and saturated fatty acids esters, palmitic acid, myristic acid, and lauric acid are responsible for α-amylase inhibition, unsaturated fatty acids for the band at RF = 0.51 and phytoecdysteroids for the band at RF = 0.53.
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Affiliation(s)
- Snezana Agatonovic-Kustrin
- A.P. Arzamastsev Department of Pharmaceutical and Toxicological Chemistry, I.M. Sechenov First Moscow State Medical University (Sechenov University), Moscow 119991, Russian Federation; School of Pharmacy and Biomedical Sciences, La Trobe University, Edwards Rd, Bendigo 3550, Australia.
| | - Galina Ramenskaya
- A.P. Arzamastsev Department of Pharmaceutical and Toxicological Chemistry, I.M. Sechenov First Moscow State Medical University (Sechenov University), Moscow 119991, Russian Federation; Scientific Center for Expert Evaluation of Medicinal Products, Ministry of Health of the Russian Federation, 8/2 Petrovskii Blvd., Moscow 127051, Russian Federation
| | - Ella Kustrin
- Department of Creative Arts and English, La Trobe University, Edwards Rd, Bendigo 3550, Australia
| | - David W Morton
- A.P. Arzamastsev Department of Pharmaceutical and Toxicological Chemistry, I.M. Sechenov First Moscow State Medical University (Sechenov University), Moscow 119991, Russian Federation; School of Pharmacy and Biomedical Sciences, La Trobe University, Edwards Rd, Bendigo 3550, Australia
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Formulation of Topical Dosage Forms Containing Synthetic and Natural Anti-Inflammatory Agents for the Treatment of Rheumatoid Arthritis. MOLECULES (BASEL, SWITZERLAND) 2020; 26:molecules26010024. [PMID: 33374575 PMCID: PMC7793088 DOI: 10.3390/molecules26010024] [Citation(s) in RCA: 6] [Impact Index Per Article: 1.2] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Subscribe] [Scholar Register] [Received: 11/30/2020] [Revised: 12/16/2020] [Accepted: 12/21/2020] [Indexed: 12/20/2022]
Abstract
Topical anti-inflammatory and analgesic effect for the treatment of rheumatoid arthritis is of major interest because of their fewer side effects compared to oral therapy. The purpose of this study was to prepare different types of topical formulations (ointments and gels) containing synthetic and natural anti-inflammatory agents with different excipients (e.g.,: surfactants, gel-forming) for the treatment of rheumatoid arthritis. The combination of Non-Steroidal Anti-Inflammatory Drugs (NSAIDs), diclofenac sodium, a topical analgesic agent methyl salicylate, and a lyophilized extract of Calendula officinalis with antioxidant effect were used in our formulations. The aim was to select the appropriate excipients and dosage form for the formulation in order to enhance the diffusion of active substances and to certify the antioxidant, analgesic, and anti-inflammatory effects of these formulations. To characterize the physicochemical properties of the formulations, rheological studies, and texture profile analysis were carried out. Membrane diffusion and permeability studies were performed with Franz-diffusion method. The therapeutic properties of the formulations have been proven by an antioxidant assay and a randomized prospective study that was carried out on 115 patients with rheumatoid arthritis. The results showed that the treatment with the gel containing diclofenac sodium, methyl salicylate, and lyophilized Calendula officinalis as active ingredients, 2-propenoic acid homopolymer (Synthalen K) as gel-forming excipient, distilled water, triethanolamine, and glycerol had a beneficial analgesic and local anti-inflammatory effect.
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Effects and Characterization of Some Topical Ointments Based on Vegetal Extracts on Incision, Excision, and Thermal Wound Models. Molecules 2020; 25:molecules25225356. [PMID: 33207838 PMCID: PMC7709045 DOI: 10.3390/molecules25225356] [Citation(s) in RCA: 7] [Impact Index Per Article: 1.4] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 09/29/2020] [Revised: 11/10/2020] [Accepted: 11/13/2020] [Indexed: 12/19/2022] Open
Abstract
The present paper aims to formulate and characterize four phytotherapeutic ointments based on Hippophae fructus, Calendulae flos, Bardanae folium, and Millefolii herba, which are included in a novel ointment base. In order to investigate the healing properties of the ointments, in vivo experimental wound models of linear incision, circular excision, and thermal burn were performed on Wistar rats. Topical treatment was performed daily for 21 days. Determination of the wound contraction rate (WCR), the period of reepithelization, and histopathological examination were achieved. Additionally, for the tested ointments, oscillatory and rotational rheological tests were carried out, and for the extracts, HPLC analysis was performed. The results demonstrate that the tested novel ointments are safe for use and the most effective ointment proved to be the one based on Arctium lappa, followed by that of Calendulae flos.
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Chanaj-Kaczmarek J, Paczkowska M, Osmałek T, Kaproń B, Plech T, Szymanowska D, Karaźniewicz-Łada M, Kobus-Cisowska J, Cielecka-Piontek J. Hydrogel Delivery System Containing Calendulae flos Lyophilized Extract with Chitosan as a Supporting Strategy for Wound Healing Applications. Pharmaceutics 2020; 12:E634. [PMID: 32645859 PMCID: PMC7407229 DOI: 10.3390/pharmaceutics12070634] [Citation(s) in RCA: 15] [Impact Index Per Article: 3.0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 05/21/2020] [Revised: 06/14/2020] [Accepted: 06/29/2020] [Indexed: 12/19/2022] Open
Abstract
Calendulae flos is a valued plant material with known anti-inflammatory and antimicrobiological properties. The limitation for its use in the treatment of chronic wounds is the lack of adhesion to the required site of action. Obtaining the Calendulae flos lyophilized extract from water-ethanolic extract allowed to prepare valuable material whose biological activity in the wound healing process was confirmed by a positive result of the scratch test. The Calendulae flos lyophilized extract was standardized for the contents of the chlorogenic acid and the narcissin. The significant potency of the Calendulae flos pharmacological activity has become the reason for studies on its novel applications in combination with the multifunctional chitosan carrier, to create a new, valuable solution in the treatment of chronic wounds. The use of chitosan as a carrier allowed for the controlled release of the chlorogenic acid and the narcissin. These substances, characterized by prolonged release from the chitosan delivery system, were identified as well permeable, based on the results of the studies of the parallel artificial membrane permeability assay (PAMPA Skin) a model simulating permeability through membrane skin. The combination of the Calendulae flos lyophilized extract and the chitosan allowed for synergy of action towards hyaluronidase inhibition and effective microbiological activity. Optimization of the hypromellose hydrogel preparation ensuring the required rheological properties necessary for the release of the chlorogenic acid and the narcissin from the chitosan delivery system, as well as demonstrated antimicrobial activity allows indicating formulations of 3% Calendulae flos lyophilized extract with chitosan 80/500 in weight ratio 1:1 and 2% or 3% hypromellose as an important support with high compliance of response and effectiveness for patients suffering from chronic wounds.
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Affiliation(s)
- Justyna Chanaj-Kaczmarek
- Department of Pharmacognosy, Poznan University of Medical Sciences, 4 Swiecickiego Street, 60781 Poznan, Poland; (J.C.-K.); (M.P.)
| | - Magdalena Paczkowska
- Department of Pharmacognosy, Poznan University of Medical Sciences, 4 Swiecickiego Street, 60781 Poznan, Poland; (J.C.-K.); (M.P.)
| | - Tomasz Osmałek
- Department of Pharmaceutical Technology, Poznan University of Medical Sciences, 6 Grunwaldzka Street, 60780 Poznan, Poland;
| | - Barbara Kaproń
- Department of Clinical Genetics, Medical University of Lublin, 11 Radziwillowska Street, 20080 Lublin, Poland;
| | - Tomasz Plech
- Department of Pharmacology, Faculty of Health Sciences, Medical University of Lublin, 4a Chodzki Street, 20093 Lublin, Poland;
| | - Daria Szymanowska
- Faculty of Food Science and Nutrition, Poznan University of Life Sciences, 31 Wojska Polskiego Street, 60-634 Poznan, Poland;
| | - Marta Karaźniewicz-Łada
- Department of Physical Pharmacy and Pharmacokinetics, Poznan University of Medical Sciences, 6 Swiecickiego Street, 60781 Poznan, Poland;
| | - Joanna Kobus-Cisowska
- Department of Gastronomy Science and Functional Foods, Poznan University of Life Sciences, Wojska Polskiego 28, 60637 Poznan, Poland;
| | - Judyta Cielecka-Piontek
- Department of Pharmacognosy, Poznan University of Medical Sciences, 4 Swiecickiego Street, 60781 Poznan, Poland; (J.C.-K.); (M.P.)
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Fiuza MFM, Maciel MF, Pereira LA, Krause LMF. Evolution of a diabetic foot wound with the use of calendula: A single‐case experimental design. Phytother Res 2020; 34:907-910. [DOI: 10.1002/ptr.6595] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 09/03/2019] [Revised: 11/16/2019] [Accepted: 11/30/2019] [Indexed: 11/11/2022]
Affiliation(s)
- Miriãn F. M. Fiuza
- Departamento de GeneticFederal University of Rio Grande do Sul Porto Alegre Brazil
- Franciscan University Santa Maria Brazil
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Hajihasani MM, Soheili V, Zirak MR, Sahebkar A, Shakeri A. Natural products as safeguards against monosodium glutamate-induced toxicity. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES 2020; 23:416-430. [PMID: 32489556 PMCID: PMC7239414 DOI: 10.22038/ijbms.2020.43060.10123] [Citation(s) in RCA: 16] [Impact Index Per Article: 3.2] [Reference Citation Analysis] [Abstract] [Key Words] [Download PDF] [Figures] [Subscribe] [Scholar Register] [Received: 09/09/2019] [Accepted: 01/04/2020] [Indexed: 12/17/2022]
Abstract
Monosodium glutamate is a sodium salt of a nonessential amino acid, L-glutamic acid, which is widely used in food industry. Glutamate plays an important role in principal brain functions including formation and stabilization of synapses, memory, cognition, learning, as well as cellular metabolism. However, ingestion of foodstuffs rich in monosodium glutamate can result in the outbreak of several health disorders such as neurotoxicity, hepatotoxicity, obesity and diabetes. The usage of medicinal plants and their natural products as a therapy against MSG used in food industry has been suggested to be protective. Calendula officinalis, Curcuma longa, Green Tea, Ginkgo biloba and vitamins are some of the main natural products with protective effect against mentioned monosodium glutamate toxicity through different mechanisms. This review provides a summary on the toxicity of monosodium glutamate and the protective effects of natural products against monosodium glutamate -induced toxicity.
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Affiliation(s)
- Mohammad Mahdi Hajihasani
- Department of Pharmaceutical Control, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
| | - Vahid Soheili
- Department of Pharmaceutical Control, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
| | - Mohammad Reza Zirak
- Department of Pharmacodynamics and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
| | - Amirhossein Sahebkar
- Neurogenic Inflammation Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
- Biotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran
- School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
| | - Abolfazl Shakeri
- Department of Pharmacognosy, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
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Fujishima MAT, Sá DMC, Lima CMDS, Bittencourt JAHM, Pereira WLA, Muribeca ADJB, e Silva CYY, da Silva MN, de Sousa FFO, dos Santos CBR, da Silva JO. Chemical profiling of Curatella americana Linn leaves by UPLC-HRMS and its wound healing activity in mice. PLoS One 2020; 15:e0225514. [PMID: 31929529 PMCID: PMC6957176 DOI: 10.1371/journal.pone.0225514] [Citation(s) in RCA: 3] [Impact Index Per Article: 0.6] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 06/16/2018] [Accepted: 11/06/2019] [Indexed: 12/11/2022] Open
Abstract
Based on ethnopharmacological studies, a lot of plants, as well as its compounds, have been investigated for the potential use as wound healing agents. In Brazil, Curatella americana is traditionally used by local people to treat wounds, ulcers and inflammations. However, to the best of our knowledge, its traditional use in the treatment of wounds has not been validated by a scientific study. Here, some compounds, many of them flavonoids, were identified in the hydroethanolic extract from the leaves of C. americana (HECA) by LC-HRMS and LC-MS/MS. Besides that, solutions containing different concentrations of HECA and a gel produced with this extract were evaluated for its antimicrobial, coagulant and wound healing activities on an excision mouse wound model as well as its acute dermal safety. A total of thirteen compounds were identified in HECA, mainly quercetin, kaempferol and glucoside derivatives of both, besides catechin and epicatechin known as wound healing agents. The group treated with 1% of HECA exhibited highest wound healing activity and best rate of wound contraction confirmed by histopathology results. The present study provides scientific evidence of, this extract (HECA) possess remarkable wound healing activity, thereby, supporting the traditional use.
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Affiliation(s)
- Mayara Amoras Teles Fujishima
- Postgraduate Program of Pharmaceutical Innovation, Federal University of Amapá, Macapá, AP, Brazil
- Laboratory of Toxicology, Department of Biological and Health Sciences, Federal University of Amapá, Macapá, AP, Brazil
- Laboratory of Modeling and Computational Chemistry, Department of Biological and Health Sciences, Federal University of Amapá, Macapá, AP, Brazil
- * E-mail:
| | - Dayse Maria Cunha Sá
- Postgraduate Program of Pharmaceutical Innovation, Federal University of Amapá, Macapá, AP, Brazil
- Laboratory of Toxicology, Department of Biological and Health Sciences, Federal University of Amapá, Macapá, AP, Brazil
| | - Carolina Miranda de Sousa Lima
- Postgraduate Program of Pharmaceutical Innovation, Federal University of Amapá, Macapá, AP, Brazil
- Laboratory of Toxicology, Department of Biological and Health Sciences, Federal University of Amapá, Macapá, AP, Brazil
| | - José Adolfo H. M. Bittencourt
- Postgraduate Program of Pharmaceutical Innovation, Federal University of Amapá, Macapá, AP, Brazil
- Laboratory of Modeling and Computational Chemistry, Department of Biological and Health Sciences, Federal University of Amapá, Macapá, AP, Brazil
| | | | | | | | | | | | - Cleydson B. R. dos Santos
- Postgraduate Program of Pharmaceutical Innovation, Federal University of Amapá, Macapá, AP, Brazil
- Laboratory of Modeling and Computational Chemistry, Department of Biological and Health Sciences, Federal University of Amapá, Macapá, AP, Brazil
| | - Jocivania Oliveira da Silva
- Postgraduate Program of Pharmaceutical Innovation, Federal University of Amapá, Macapá, AP, Brazil
- Laboratory of Toxicology, Department of Biological and Health Sciences, Federal University of Amapá, Macapá, AP, Brazil
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Species-Specific Antioxidant Power and Bioactive Properties of the Extracts Obtained from Wild Mediterranean Calendula Spp. (Asteraceae). APPLIED SCIENCES-BASEL 2019. [DOI: 10.3390/app9214627] [Citation(s) in RCA: 12] [Impact Index Per Article: 2.0] [Reference Citation Analysis] [Abstract] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 11/16/2022]
Abstract
In this study we focused on four taxa of the genus Calendula (C. maritima, C. suffruticosa subsp. fulgida, C. arvensis, and the hybrid between the first two ones), collected in Mediterranean area (Sicily). Six extracts for each species were obtained using solvents with increasing polarity (hexane, ethanol 80%, acetone 70%, and water) and through extraction by supercritical fluids (SFE). It has been observed that the solvent with the highest extraction efficiency was ethanol 80% for all species. However, SFE extracts showed high antioxidant activity comparable to the ethanol 80% extract (polyphenol, DPPH, and reducing power method). These findings were confirmed by in vitro analysis (MTT assay) where it was observed that the tested concentration (24 µg/mL), obtained from ethanol 80% and SFE extracts, showed a protective effect comparable to that induced by a synthetic antioxidant. Extraction with SFE ensured a great selectivity by avoiding the use of toxic organic solvents and thus consisted of a promising technique for sustainable production of Calendula extracts.
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Wound Healing and the Use of Medicinal Plants. EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE 2019; 2019:2684108. [PMID: 31662773 PMCID: PMC6778887 DOI: 10.1155/2019/2684108] [Citation(s) in RCA: 103] [Impact Index Per Article: 17.2] [Reference Citation Analysis] [Abstract] [Track Full Text] [Download PDF] [Figures] [Subscribe] [Scholar Register] [Received: 03/01/2019] [Revised: 05/03/2019] [Accepted: 09/01/2019] [Indexed: 02/06/2023]
Abstract
Cutaneous wound healing is the process by which skin repairs itself. It is generally accepted that cutaneous wound healing can be divided into 4 phases: haemostasis, inflammation, proliferation, and remodelling. In humans, keratinocytes re-form a functional epidermis (reepithelialization) as rapidly as possible, closing the wound and reestablishing tissue homeostasis. Dermal fibroblasts migrate into the wound bed and proliferate, creating “granulation tissue” rich in extracellular matrix proteins and supporting the growth of new blood vessels. Ultimately, this is remodelled over an extended period, returning the injured tissue to a state similar to that before injury. Dysregulation in any phase of the wound healing cascade delays healing and may result in various skin pathologies, including nonhealing, or chronic ulceration. Indigenous and traditional medicines make extensive use of natural products and derivatives of natural products and provide more than half of all medicines consumed today throughout the world. Recognising the important role traditional medicine continues to play, we have undertaken an extensive survey of literature reporting the use of medical plants and plant-based products for cutaneous wounds. We describe the active ingredients, bioactivities, clinical uses, formulations, methods of preparation, and clinical value of 36 medical plant species. Several species stand out, including Centella asiatica, Curcuma longa, and Paeonia suffruticosa, which are popular wound healing products used by several cultures and ethnic groups. The popularity and evidence of continued use clearly indicates that there are still lessons to be learned from traditional practices. Hidden in the myriad of natural products and derivatives from natural products are undescribed reagents, unexplored combinations, and adjunct compounds that could have a place in the contemporary therapeutic inventory.
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Karimi E, Gharib B, Rostami N, Navidpour L, Afshar M. Clinical efficacy of a topical polyherbal formulation in the management of fluorouracil -associated hand-foot syndrome. J Herb Med 2019. [DOI: 10.1016/j.hermed.2019.100270] [Citation(s) in RCA: 4] [Impact Index Per Article: 0.7] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 12/25/2022]
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Pedram Rad Z, Mokhtari J, Abbasi M. Calendula officinalis extract/PCL/Zein/Gum arabic nanofibrous bio-composite scaffolds via suspension, two-nozzle and multilayer electrospinning for skin tissue engineering. Int J Biol Macromol 2019; 135:530-543. [DOI: 10.1016/j.ijbiomac.2019.05.204] [Citation(s) in RCA: 90] [Impact Index Per Article: 15.0] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 05/01/2019] [Revised: 05/27/2019] [Accepted: 05/27/2019] [Indexed: 12/12/2022]
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Dos Santos DS, Barreto RDSS, Serafini MR, Gouveia DN, Marques RS, Nascimento LDC, Nascimento JDC, Guimarães AG. Phytomedicines containing Matricaria species for the treatment of skin diseases: A biotechnological approach. Fitoterapia 2019; 138:104267. [PMID: 31319107 DOI: 10.1016/j.fitote.2019.104267] [Citation(s) in RCA: 4] [Impact Index Per Article: 0.7] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 05/07/2019] [Revised: 07/11/2019] [Accepted: 07/12/2019] [Indexed: 01/09/2023]
Abstract
Skin diseases have a notable impact on the life of the affected and in their health conditions. In order to allow a more effective and economical treatment for such disorders, new therapeutic approaches have been continuously investigated. Due to its high therapeutic and phytochemical potential, Matricaria species emerges as a pleasant alternative, since it is an important source of bioactive secondary metabolites suitable for the treatment of varied skin diseases. Therefore, this review aimed to catalog inventions that have used Matricaria species as the active component for skin disease treatment in order to assess the status of the technological development of the fitomedicines. For this, a search of patents was performed in four specialized patent database, which have reported the discovery of pharmaceutical bioproducts that used Matricaria species in its composition for skin treatment. Therefore, it is possible to notice that the pharmaceutical industry has driven efforts and investment to produce medicines for the dermatopathies, using species of this genus as an active principle. Besides, a trend of growth has been identified for the last years, which is accompanied by the continuous publication of scientific articles on the subject. It is known that a long journey is traversed between the scientific findings and their applications in the pharmaceutical market, creating the perspective that new Matricaria-based medicines may reach the pharmaceutical market in the coming years.
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Affiliation(s)
- Damaris Silva Dos Santos
- Programa de Pós-graduação em Ciências Aplicadas à Saúde, Universidade Federal de Sergipe, Lagarto, Sergipe, Brazil
| | - Rosana de Souza Siqueira Barreto
- Programa de Pós-graduação em Ciências Aplicadas à Saúde, Universidade Federal de Sergipe, Lagarto, Sergipe, Brazil; Departmento de Educação em Saúde, Universidade Federal de Sergipe, Lagarto, Sergipe, Brazil
| | - Mairim Russo Serafini
- Departmento de Farmácia, Universidade Federal de Sergipe, São Cristóvão, Sergipe, Brazil
| | - Daniele Nascimento Gouveia
- Programa de Pós-graduação em Ciências Aplicadas à Saúde, Universidade Federal de Sergipe, Lagarto, Sergipe, Brazil
| | - Raquel Souza Marques
- Programa de Pós-graduação em Ciências Odontológicas, Universidade do Estado de São Paulo, Araraquara, São Paulo, Brazil
| | | | | | - Adriana Gibara Guimarães
- Programa de Pós-graduação em Ciências Aplicadas à Saúde, Universidade Federal de Sergipe, Lagarto, Sergipe, Brazil; Departmento de Educação em Saúde, Universidade Federal de Sergipe, Lagarto, Sergipe, Brazil.
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Givol O, Kornhaber R, Visentin D, Cleary M, Haik J, Harats M. A systematic review of
Calendula officinalis
extract for wound healing. Wound Repair Regen 2019; 27:548-561. [DOI: 10.1111/wrr.12737] [Citation(s) in RCA: 73] [Impact Index Per Article: 12.2] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 02/16/2019] [Revised: 05/15/2019] [Accepted: 05/20/2019] [Indexed: 12/26/2022]
Affiliation(s)
- Or Givol
- Sackler School of MedicineTel Aviv University Tel Aviv Israel
- Department of Plastic and Reconstructive SurgerySheba Medical Center Tel Hashomer Israel
| | - Rachel Kornhaber
- Department of Plastic and Reconstructive SurgerySheba Medical Center Tel Hashomer Israel
- College of Health and MedicineUniversity of Tasmania Sydney New South Wales Australia
| | - Denis Visentin
- College of Health and MedicineUniversity of Tasmania Sydney New South Wales Australia
| | - Michelle Cleary
- College of Health and MedicineUniversity of Tasmania Sydney New South Wales Australia
| | - Josef Haik
- Sackler School of MedicineTel Aviv University Tel Aviv Israel
- Department of Plastic and Reconstructive SurgerySheba Medical Center Tel Hashomer Israel
- College of Health and MedicineUniversity of Tasmania Sydney New South Wales Australia
- University of Notre Dame Australia Fremantle Western Australia Australia
- Talpiot Leadership Program, Sheba Medical Center Tel Hashomer Israel
| | - Moti Harats
- Sackler School of MedicineTel Aviv University Tel Aviv Israel
- Department of Plastic and Reconstructive SurgerySheba Medical Center Tel Hashomer Israel
- University of Notre Dame Australia Fremantle Western Australia Australia
- Talpiot Leadership Program, Sheba Medical Center Tel Hashomer Israel
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Simultaneous Supercritical Fluid Extraction of Heather (Calluna vulgaris L.) and Marigold (Calendula officinalis L.) and Anti-Inflammatory Activity of the Extracts. APPLIED SCIENCES-BASEL 2019. [DOI: 10.3390/app9112245] [Citation(s) in RCA: 5] [Impact Index Per Article: 0.8] [Reference Citation Analysis] [Abstract] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 12/20/2022]
Abstract
Heather (Calluna vulgaris L.) and marigold (Calendula officinalis L.) are two rich sources of bioactive pentacyclic triterpenes. The supercritical carbon dioxide (SCCO2) extraction of these two plants was explored at a pressure range of 25–50 MPa, 50 °C, with or without fractionation, different extraction times (1.5–4.0 h) and using ethanol as a co-solvent (0 and 10% w/w). In order to determine potential synergisms, a combined extraction (heather + marigold 50:50) was also studied. In general, higher extraction yields were achieved when the co-solvent was added. Higher concentrations of total triterpenic acids were obtained in heather extracts, specially using ethanol. The co-solvent did not increase the terpene concentration in marigold extracts. For the combined extraction, an antagonist effect in the triterpene concentration was observed in absence of a co-solvent, whereas a synergistic effect was exhibited in its presence, especially for ursolic acid. In general, the extracts showed a certain anti-inflammatory effect, although a straight correlation with the analyzed triterpenic acids concentration was not exhibited. Moreover, the combined extraction exposed a similar anti-inflammatory activity in comparison with the individual plant extracts.
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Tresch M, Mevissen M, Ayrle H, Melzig M, Roosje P, Walkenhorst M. Medicinal plants as therapeutic options for topical treatment in canine dermatology? A systematic review. BMC Vet Res 2019; 15:174. [PMID: 31133058 PMCID: PMC6537371 DOI: 10.1186/s12917-019-1854-4] [Citation(s) in RCA: 15] [Impact Index Per Article: 2.5] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 05/04/2018] [Accepted: 03/27/2019] [Indexed: 12/14/2022] Open
Abstract
Background Medicinal plants have been used traditionally since centuries for wound care and treatment of skin diseases both in human and animals. Skin diseases are one of the most common reasons for owners to take their dog to the veterinarian. The demands for treatment and prophylaxis of these diseases are broad. A wide range of bacteria including antibiotic-resistant bacteria can be involved, making the treatment challenging and bear an anthropo-zoonotic potential. The aim of this review is to systematically evaluate based on recent scientific literature, the potential of four medicinal plants to enrich the therapeutic options in pyoderma, canine atopic dermatitis, otitis externa, wounds and dermatophytosis in dogs. Results Based on four books and a survey among veterinarians specialized in phytotherapy, four medicinal plants were chosen as the subject of this systematic review: Calendula officinalis L. (Marigold), Hypericum perforatum L. agg. (St. John’s Wort), Matricaria chamomilla L. (syn. Matricaria recutita L., Chamomile) and Salvia officinalis L. (Sage). According to the PRISMA statement through literature research on two online databases a total of 8295 publications was screened and narrowed down to a final 138 publications for which full-text documents were analyzed for its content resulting in a total of 145 references (21 clinical, 24 in vivo and 100 in vitro references). Conclusions All four plants were proven to have antibacterial and antifungal effects of a rather broad spectrum including antibiotic-resistant bacteria. This makes them an interesting new option for the treatment of pyoderma, otitis externa, infected wounds and dermatophytosis. Marigold, St. John’s Wort and Chamomile showed wound-healing properties and are thus promising candidates in line to fill the therapeutic gap in canine wound-healing agents. St. John’s Wort and Chamomile also showed anti-inflammatory and other beneficial effects on healthy skin. Due to the wide range of beneficial effects of these medicinal plants, they should be taken into account for the treatment of dermatologic diseases in dogs at least in future clinical research. Electronic supplementary material The online version of this article (10.1186/s12917-019-1854-4) contains supplementary material, which is available to authorized users.
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Affiliation(s)
- Milena Tresch
- Division Veterinary Pharmacology & Toxicology, Department Clinical Research and Veterinary Public Health, Vetsuisse Faculty, University of Bern, Laenggassstrasse 124, 3012, Bern, Switzerland
| | - Meike Mevissen
- Division Veterinary Pharmacology & Toxicology, Department Clinical Research and Veterinary Public Health, Vetsuisse Faculty, University of Bern, Laenggassstrasse 124, 3012, Bern, Switzerland
| | - Hannah Ayrle
- Department of Livestock Sciences, Research Institute of Organic Agriculture (FiBL), Ackerstrasse 113, Postbox 219, 5070, Frick, Switzerland
| | - Matthias Melzig
- Dahlem Centre of Plant Sciences, Institute of Pharmacy, Freie Universität Berlin, Koenigin-Luise-Strasse 2+4, 14195, Berlin, Germany
| | - Petra Roosje
- Division of Clinical Dermatology, Department of Clinical Veterinary Medicine, Vetsuisse Faculty, University of Bern, Laenggassstrasse 124, 3012, Bern, Switzerland
| | - Michael Walkenhorst
- Department of Livestock Sciences, Research Institute of Organic Agriculture (FiBL), Ackerstrasse 113, Postbox 219, 5070, Frick, Switzerland.
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Pedram Rad Z, Mokhtari J, Abbasi M. Preparation and characterization of Calendula officinalis-loaded PCL/gum arabic nanocomposite scaffolds for wound healing applications. IRANIAN POLYMER JOURNAL 2018. [DOI: 10.1007/s13726-018-0674-x] [Citation(s) in RCA: 35] [Impact Index Per Article: 5.0] [Reference Citation Analysis] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 01/22/2023]
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Cruceriu D, Balacescu O, Rakosy E. Calendula officinalis: Potential Roles in Cancer Treatment and Palliative Care. Integr Cancer Ther 2018; 17:1068-1078. [PMID: 30289008 PMCID: PMC6247547 DOI: 10.1177/1534735418803766] [Citation(s) in RCA: 27] [Impact Index Per Article: 3.9] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/17/2022] Open
Abstract
A continuous challenge in cancer management is to improve treatment efficacy and
to diminish its side effects. Consequently, new conventional and unconventional
drugs and bioactive compounds from plants are constantly developed,
characterized, and used for in vitro and in vivo models. This review focuses on
the antitumor properties of Calendula officinalis, its
biological and molecular effects in tumor cells and animal models, as well as
its role in cancer palliative care. A systematic review of studies describing
the cytotoxic role of C officinalis and its therapeutic role on
cancer cells were carried out using the PubMed database. Albeit C
officinalis extracts have cytotoxic activity toward different
cancer cell lines, a high grade of variation between studies was observed,
depending on plant organ subjected to extraction, extraction method, and the
cancer cell lines used for each study. Nevertheless, its cytotoxic activity is
related to a few bioactive compounds, presenting multiple roles in both
activation of proapoptotic proteins and decreasing the expression of the
proteins that inhibit cell death. Moreover, due to its anti-genotoxic/protective
as well as antitumor and antimetastatic effects proven in animal models,
C officinalis could have important future implications in
developing novel cancer treatment strategies, while until now it has been used
especially for diminishing the side effects of radiotherapy.
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Affiliation(s)
- Daniel Cruceriu
- 1 The Oncology Institute "Prof. Dr. Ion Chiricuta," Cluj-Napoca, Romania.,2 "Babes-Bolyai" University, Cluj-Napoca, Romania
| | - Ovidiu Balacescu
- 1 The Oncology Institute "Prof. Dr. Ion Chiricuta," Cluj-Napoca, Romania.,3 University of Medicine and Pharmacy "Iuliu Hatieganu," Cluj-Napoca, Romania
| | - Elena Rakosy
- 2 "Babes-Bolyai" University, Cluj-Napoca, Romania
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Carvalho AR, Diniz RM, Suarez MAM, Figueiredo CSSES, Zagmignan A, Grisotto MAG, Fernandes ES, da Silva LCN. Use of Some Asteraceae Plants for the Treatment of Wounds: From Ethnopharmacological Studies to Scientific Evidences. Front Pharmacol 2018; 9:784. [PMID: 30186158 PMCID: PMC6110936 DOI: 10.3389/fphar.2018.00784] [Citation(s) in RCA: 26] [Impact Index Per Article: 3.7] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 03/24/2018] [Accepted: 06/27/2018] [Indexed: 12/11/2022] Open
Abstract
Severe wounds result in large lesions and/or loss of function of the affected areas. The treatment of wounds has challenged health professionals due to its complexity, especially in patients with chronic diseases (such as diabetes), and the presence of pathogens such as Staphylococcus aureus and Pseudomonas aeruginosa. Taking this into consideration, the development of new therapies for wound healing requires immediate attention. Ethnopharmacological studies performed in different countries have shown the use of several plants from the Asteraceae family as wound-healing agents. Evidences gained from the traditional medicine have opened new ways for the development of novel and more efficient therapies based on the pharmacological properties of these plants. In this article, we discuss the literature data on the use of Asteraceae plants for the treatment of wounds, based on the ethnopharmacological relevance of each plant. Special attention was given to studies showing the mechanisms of action of Asteraceae-derived compounds and clinical trials. Ageratina pichinchensis (Kunth) R.M. King and H. Rob. and Calendula officinalis L. preparations/compounds were found to show good efficacy when assessed in clinical trials of complicated wounds, including venous leg ulcers and foot ulcers of diabetic patients. The compounds silibinin [from Silybum marianum (L.) Gaertn.] and jaceosidin (from Artemisia princeps Pamp.) were identified as promising compounds for the treatment of wounds. Overall, we suggest that Asteraceae plants represent important sources of compounds that may act as new and efficient healing products.
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Affiliation(s)
| | - Roseana M Diniz
- Programa de Pós-Graduação, Universidade Ceuma, São Luís, Brazil
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Mishra AK, Mishra A, Chattopadhyay P. Screening of acute and sub-chronic dermal toxicity of Calendula officinalis L essential oil. Regul Toxicol Pharmacol 2018; 98:184-189. [PMID: 30075180 DOI: 10.1016/j.yrtph.2018.07.027] [Citation(s) in RCA: 16] [Impact Index Per Article: 2.3] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 02/22/2017] [Revised: 01/29/2018] [Accepted: 07/30/2018] [Indexed: 11/18/2022]
Abstract
The objective of the study is to access the safety of Calendula essential oil by studying acute and sub-chronic dermal toxicity. The dermal toxicities of Calendula essential oil were evaluated in accordance with OECD guidelines number 402 and 411 respectively. The animals were exposed to Calendula officinalis (CO) essential oil dose of 20 mL/kg body weight for acute dermal toxicity, whereas for dermal sub-chronic toxicity study, rats were exposed to CO oil 2.5, 5 and 10 mL/kg body weight, respectively, for 7 times in a week for 90 days. The parameters studies included CNS stimulation, depression, hematological parameters (RBC, WBC, Hb, Lymphocyte % etc), biochemical parameters (total protein, albumin, total bilirubin, ALP, AST, etc), relative organ weight, necropsy and histopathology. In toxicity studies, all animals exhibited normal behavior without any change in hematology, blood biochemistry, necroscopical and histopathology. The no observed effect level (NOEL) and no observed adverse effect level (NOAEL) of CO oil were 2.5 and 10 mg/kg/day, respectively. CO oil is under the herbal medicinal product according to the European Medicines Agency with the claim of an LD50 value of 20 mL/kg body weight. The result indicates that CO essential oil did not produce any significant toxic effects.
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Affiliation(s)
- Arun K Mishra
- Central Facility of Instrumentation, Faculty of Pharmacy, IFTM University, Lodipur-Rajput, Moradabad, 244001, India.
| | - Amrita Mishra
- Central Facility of Instrumentation, Faculty of Pharmacy, IFTM University, Lodipur-Rajput, Moradabad, 244001, India
| | - Pronobesh Chattopadhyay
- Pharmaceutical Technology Division, Defense Research Laboratory, DRDO, Tezpur, 784001, India
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Marmitt DJ, Bitencourt S, Silva ADCE, Rempel C, Goettert MI. The healing properties of medicinal plants used in the Brazilian public health system: a systematic review. J Wound Care 2018; 27:S4-S13. [DOI: 10.12968/jowc.2018.27.sup6.s4] [Citation(s) in RCA: 11] [Impact Index Per Article: 1.6] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 12/22/2022]
Affiliation(s)
- Diorge Jônatas Marmitt
- Laboratório de Cultura de Células, Programa de Pós-graduação em Biotecnologia, Universidade do Vale do Taquari (Univates), Lajeado, RS, Brazil
| | - Shanna Bitencourt
- Laboratório de Cultura de Células, Programa de Pós-graduação em Biotecnologia, Universidade do Vale do Taquari (Univates), Lajeado, RS, Brazil
| | | | - Claudete Rempel
- Programa de Pós-graduação em Ambiente e Desenvolvimento, Universidade do Vale do Taquari (Univates), Lajeado, RS, Brazil
| | - Márcia Inês Goettert
- Laboratório de Cultura de Células, Programa de Pós-graduação em Biotecnologia, Universidade do Vale do Taquari (Univates), Lajeado, RS, Brazil
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Menon DN, Teixeira L, Paurosi NB, Barros ME. Effects of heparin and hyperbaric oxygenation on necrosis reduction in an animal model for degloving injuries. Rev Col Bras Cir 2018; 44:64-71. [PMID: 28489213 DOI: 10.1590/0100-69912017001014] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.1] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 09/29/2016] [Accepted: 11/03/2016] [Indexed: 11/22/2022] Open
Abstract
Objective: to evaluate the efficacy of the treatment with hyperbaric oxygen therapy or with topical and intralesional heparin in an animal model of degloving lesions. Methods: we conducted an experimental study with adult, male Wistar rats submitted to degloving of the left hind limb and divided into four groups according to the treatment: Group 1 (control) - without treatment; Group 2 (Heparin) - intralesional application at the time of surgery and topically, in the postoperative period, with heparin spray 10,000IU/mL; Group 3 (hyperbaric oxygenation) - daily sessions of 30 minutes in a hyperbaric chamber with 100% oxygen and 2 ATA pressure; Group 4 (positive control) - administration of a single dose of 45 mg/kg of intraperitoneal allopurinol. On the seventh day, we killed the animals, removed the cutaneous flaps and measured the total and necrotic areas, as well as computed the percentage of necrotic area. Results: the mean percentage of necrosis in the control group was 56.03%; in the positive control group it was 51.36% (p<0.45); in the heparin group, 42.10% (p<0.07); and in the hyperbaric oxygen therapy group, 31.58% (p<0.01) . Conclusion: both hyperbaric oxygen and heparin therapies were effective in reducing the percentage of necrosis in the model studied, although only the hyperbaric oxygenation showed statistical significance. Objetivos: avaliar a eficácia do tratamento com oxigenoterapia hiperbárica ou com heparina tópica e intralesional em modelo animal de desluvamentos. Métodos: estudo experimental, com ratos adultos machos Wistar, submetidos a desluvamento do membro posterior esquerdo e divididos em quatro grupos, de acordo com o tratamento: Grupo 1 (controle) - sem tratamento; Grupo 2 (Heparina) - aplicação intralesional no momento da cirurgia e tópica, no pós operatório, com spray de heparina 10.000UI/mL; Grupo 3 (oxigenação hiperbárica) - sessões diárias de 30 minutos em câmara hiperbárica com 100% de oxigênio e 2 ATA de pressão; Grupo 4 (controle positivo) - administração de dose única de 45mg/kg de alopurionol intraperitoneal. No sétimo dia os animais foram mortos e os retalhos cutâneos foram retirados e realizadas medidas das áreas total e necrótica, bem como cálculo da porcentagem da área de necrose. Resultados: a média da porcentagem de necrose do grupo controle foi 56,03%; no grupo controle positivo, 51,36% (p≤0,45); no grupo da heparina, 42,10% (p≤0,07) e no grupo da oxigenoterapia hiperbárica, 31,58% (p≤0,01). Conclusão: tanto a oxigenoterapia hiperbárica quanto a terapia com heparina mostraram-se eficazes na redução do percentual de necrose no modelo estudado, embora neste trabalho apenas a oxigenação hiperbárica tenha demonstrado significância estatística.
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Affiliation(s)
- Douglas Neumar Menon
- - Federal University of Grande Dourados, Faculty of Health Sciences, Dourados, Mato Grosso do Sul State, Brazil
| | - Letícia Teixeira
- - Federal University of Grande Dourados, Faculty of Health Sciences, Dourados, Mato Grosso do Sul State, Brazil
| | - Natalha Bristot Paurosi
- - Federal University of Grande Dourados, Faculty of Health Sciences, Dourados, Mato Grosso do Sul State, Brazil
| | - Marcio Eduardo Barros
- - Federal University of Grande Dourados, Faculty of Health Sciences, Dourados, Mato Grosso do Sul State, Brazil
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Artem Ataide J, Caramori Cefali L, Machado Croisfelt F, Arruda Martins Shimojo A, Oliveira-Nascimento L, Gava Mazzola P. Natural actives for wound healing: A review. Phytother Res 2018; 32:1664-1674. [PMID: 29722075 DOI: 10.1002/ptr.6102] [Citation(s) in RCA: 23] [Impact Index Per Article: 3.3] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 11/16/2017] [Revised: 03/29/2018] [Accepted: 04/05/2018] [Indexed: 12/15/2022]
Abstract
Nature has been a source of medicinal treatments for thousands of years, with the use of plants as prototypes for drug development and for the extraction of active compounds. Skin injuries occur regularly in everyday life, and the human skin has the ability to promote repair spontaneously under healthy conditions. However, some intrinsic and external factors may interfere with skins' natural ability, leading to nonhealing lesions and chronic wounds, which directly affect health and quality of life. Thus, attention should be given to this health problem, using an appropriated management when necessary. In this scenario, phytotherapy may be an option for cutaneous wound treatment, although further high-quality studies are needed to firmly establish the clinical efficacy of plants. This article reviews traditionally used natural actives for wound healing, highlighting their characteristics and mode of action.
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Affiliation(s)
- Janaína Artem Ataide
- Graduate Program in Medical Sciences, School of Medical Sciences, University of Campinas (UNICAMP), Campinas, Brazil
| | - Letícia Caramori Cefali
- Graduate Program in Biosciences and Technology of Bioactive Products, Institute of Biology, University of Campinas (UNICAMP), Campinas, Brazil
| | - Fernanda Machado Croisfelt
- Graduate Program in Biosciences and Technology of Bioactive Products, Institute of Biology, University of Campinas (UNICAMP), Campinas, Brazil
| | - Andréa Arruda Martins Shimojo
- Department of Engineering of Materials and Bioprocesses, School of Chemical Engineering, University of Campinas (UNICAMP), Campinas, Brazil
| | | | - Priscila Gava Mazzola
- Faculty of Pharmaceutical Sciences, University of Campinas (UNICAMP), Campinas, Brazil
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Ippolito E, Floreno B, Rinaldi CG, Trodella L, Meroni FL, Iurato A, D'Angelillo RM, Ramella S, Fiore M. Efficacy of a Propolis-Based Syrup (FARINGEL) in Preventing Radiation-Induced Esophagitis in Locally Advanced Lung Cancer. Chemotherapy 2018; 63:76-82. [PMID: 29554652 DOI: 10.1159/000487897] [Citation(s) in RCA: 6] [Impact Index Per Article: 0.9] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 01/31/2018] [Accepted: 02/21/2018] [Indexed: 12/19/2022]
Abstract
AIM To evaluate the efficacy of a propolis-based syrup, FARINGEL®, in preventing radiation-induced esophagitis in locally advanced lung cancer patients. METHODS Patients were treated with concurrent chemoradiotherapy (CRT) using involved-field radiotherapy (RT). Every patient received FARINGEL at the beginning of CRT until the first follow-up. The data of the study group were compared with the data of a control group treated without the administration of the syrup. RESULTS Forty-five patients were enrolled. Forty-one (91.1%) completed the protocol and were evaluable for esophagitis. Grade ≥2 toxicity occurred in 9/41 patients (22%). No differences in overall toxicity were detected between the study group and the control group (n = 55, 60.9 vs. 54.5%; p = ns). Grade 2-3 esophagitis was lower in the study group in comparison with the control group (22 and 38%, respectively), but statistical significance was not reached (p = 0.09). However, the onset of grade ≥2 esophagitis was delayed in the study group compared to the control group, occurring at higher doses of RT (41.8 vs. 25.4 Gy; p < 0.001). Furthermore, the mean number of interruption days for esophagitis was lower in the study group than in the control group (0.6 ± 2.0 vs. 2.1 ± 3.6; p = 0.025). CONCLUSION FARINGEL was well-tolerated and delayed esophagitis that was induced by CRT for locally advanced lung cancer.
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Jarić S, Kostić O, Mataruga Z, Pavlović D, Pavlović M, Mitrović M, Pavlović P. Traditional wound-healing plants used in the Balkan region (Southeast Europe). JOURNAL OF ETHNOPHARMACOLOGY 2018; 211:311-328. [PMID: 28942136 DOI: 10.1016/j.jep.2017.09.018] [Citation(s) in RCA: 94] [Impact Index Per Article: 13.4] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 06/07/2017] [Revised: 08/28/2017] [Accepted: 09/18/2017] [Indexed: 05/24/2023]
Abstract
ETHNOPHARMACOLOGICAL RELEVANCE The geographical and ecological specificity of the Balkan Peninsula has resulted in the development of a distinct diversity of medicinal plants. In the traditional culture of the Balkan peoples, plants have medicinal, economic and anthropological/cultural importance, which is reflected in the sound knowledge of their diversity and use. This study analyses the traditional use of medicinal plants in the treatment of wounds and the pharmacological characteristics of the most frequently used species. MATERIALS AND METHODS A detailed analysis of the literature related to ethnobhe uses of medicinal plants in the Balkan region was carried out. Twenty-five studies were analysed and those plants used for the treatment of wounds were singled out. RESULT An ethnobotanical analysis showed that 128 plant species (105 wild, 22 cultivated and 1 wild/cultivated) are used in the treatment of wounds. Their application is external, in the form of infusions, decoctions, tinctures, syrups, oils, ointments, and balms, or direct to the skin. Among those plants recorded, the most commonly used are Plantago major, Hypericum perforatum, Plantago lanceolata, Achillea millefolium, Calendula officinalis, Sambucus nigra, Tussilago farfara and Prunus domestica. The study showed that the traditional use of plants in wound healing is confirmed by in vitro and/or in vivo studies for P. major and P. lanceolata (3 laboratory studies for P. major and 2 for P. lanceolata), H. perforatum (5 laboratory studies and 3 clinical trials), A. millefolium (3 laboratory studies and one clinical trial), C. officinalis (6 laboratory studies and 1 clinical trial), S. nigra (3 laboratory studies) and T. farfara (one laboratory study). CONCLUSION The beneficial effects of using medicinal plants from the Balkan region to heal wounds according to traditional practices have been proven in many scientific studies. However, information on the quantitative benefits to human health of using herbal medicines to heal wounds is still scarce or fragmented, hindering a proper evaluation. Therefore, further studies should be aimed at isolating and identifying specific active substances from plant extracts, which could also reveal compounds with more valuable therapeutic properties. Furthermore, additional reliable clinical trials are needed to confirm those experiences encountered when using traditional medicines. A combination of traditional and modern knowledge could result in new wound-healing drugs with a significant reduction in unwanted side effects.
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Affiliation(s)
- Snežana Jarić
- Department of Ecology, Institute for Biological Research 'Siniša Stanković', University of Belgrade, Bulevar Despota Stefana 142, 11060 Belgrade, Serbia.
| | - Olga Kostić
- Department of Ecology, Institute for Biological Research 'Siniša Stanković', University of Belgrade, Bulevar Despota Stefana 142, 11060 Belgrade, Serbia
| | - Zorana Mataruga
- Department of Ecology, Institute for Biological Research 'Siniša Stanković', University of Belgrade, Bulevar Despota Stefana 142, 11060 Belgrade, Serbia
| | - Dragana Pavlović
- Department of Ecology, Institute for Biological Research 'Siniša Stanković', University of Belgrade, Bulevar Despota Stefana 142, 11060 Belgrade, Serbia
| | - Marija Pavlović
- Department of Ecology, Institute for Biological Research 'Siniša Stanković', University of Belgrade, Bulevar Despota Stefana 142, 11060 Belgrade, Serbia
| | - Miroslava Mitrović
- Department of Ecology, Institute for Biological Research 'Siniša Stanković', University of Belgrade, Bulevar Despota Stefana 142, 11060 Belgrade, Serbia
| | - Pavle Pavlović
- Department of Ecology, Institute for Biological Research 'Siniša Stanković', University of Belgrade, Bulevar Despota Stefana 142, 11060 Belgrade, Serbia
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Skin Wound Healing Potential and Mechanisms of the Hydroalcoholic Extract of Leaves and Oleoresin of Copaifera langsdorffii Desf. Kuntze in Rats. EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE 2017; 2017:6589270. [PMID: 28928790 PMCID: PMC5592006 DOI: 10.1155/2017/6589270] [Citation(s) in RCA: 20] [Impact Index Per Article: 2.5] [Reference Citation Analysis] [Abstract] [Track Full Text] [Download PDF] [Figures] [Subscribe] [Scholar Register] [Received: 04/20/2017] [Revised: 06/25/2017] [Accepted: 07/04/2017] [Indexed: 12/22/2022]
Abstract
The wound healing is a complex process which, sometimes, can be a problem in public health because of the possibility of physical disability or even death. Due to the lack of a gold standard drug in skin wound treatment and aiming at the discovery of new treatments in skin repair and the mechanisms involved in the process, we used oleoresin (OR) from Copaifera langsdorffii and hydroalcoholic extract of the leaves (EH) to treat rat skin wounds. For that, male Wistar rats were divided into groups (n = 8): Lanette, Collagenase, 10% EH, or 10% OR and, after anesthesia, one wound of 2 cm was made in the back of animals. The wounds were treated once a day for 3, 7, or 14 days and the wound areas were measured. The rats were euthanized and skin samples destined to biochemical, molecular, and immunohistochemical analysis. The results showed a macroscopic retraction of the wounds of 10% EH and 10% OR creams and both treatments showed anti-inflammatory activity. Molecular and immunohistochemical results demonstrated the activity of Copaifera langsdorffii creams in angiogenesis, reepithelialization, wound retraction, and remodeling mechanisms.
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