Reference Citation Analysis: Find an Article, Find a Category, Find a Journal, Find a Scholar

For: Kopic S, Geibel JP. Gastric acid, calcium absorption, and their impact on bone health. Physiol Rev 2013;93:189-268. [PMID: 23303909 DOI: 10.1152/physrev.00015.2012] [Citation(s) in RCA: 110] [Impact Index Per Article: 9.2] [Reference Citation Analysis] [What about the content of this article? (0)] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 12/11/2022] Open

For:	Kopic S, Geibel JP. Gastric acid, calcium absorption, and their impact on bone health. Physiol Rev 2013;93:189-268. [PMID: 23303909 DOI: 10.1152/physrev.00015.2012] [Citation(s) in RCA: 110] [Impact Index Per Article: 9.2] [Reference Citation Analysis] [What about the content of this article? (0)] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 12/11/2022] Open

Number

Cited by Other Article(s)

Rossip M, Lorenz FJ, Goyal N, Goldenberg D. Hypocalcemia After Thyroidectomy in Patients Taking Proton Pump Inhibitors. Laryngoscope Investig Otolaryngol 2025;10:e70163. [PMID: 40416776 PMCID: PMC12102693 DOI: 10.1002/lio2.70163] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Grants] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 10/14/2024] [Revised: 03/13/2025] [Accepted: 05/02/2025] [Indexed: 05/27/2025] Open

Yang L, Zhou H, Wang Q, Wang Z, Bai L, Wei J, Song L, Zhang F, Tian X, Liang T. Prevention of osteoporosis in ovariectomized mice with calcium yak caseinate. J Dairy Sci 2025;108:4544-4557. [PMID: 40139370 DOI: 10.3168/jds.2024-25711] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 09/16/2024] [Accepted: 02/05/2025] [Indexed: 03/29/2025]

Thammayon N, Wongdee K, Teerapornpuntakit J, Jantarajit W, Panmanee J, Patigo A, Saparpakorn P, Tanramluk D, Charoenphandhu N. Calcium transport across intestinal epithelia depends on voltage-gated sodium channels and endocannabinoid system. Biochem Biophys Res Commun 2025;758:151635. [PMID: 40120346 DOI: 10.1016/j.bbrc.2025.151635] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 10/22/2024] [Revised: 03/03/2025] [Accepted: 03/14/2025] [Indexed: 03/25/2025]

Wu W, Yu M, Liu Y, Wang X, Wang L, Wei Q, Feng H, Chen J. Effects of a functional milk powder supplemented with rhizoma drynariae extracts on bone health in growing and ovariectomized rats: More effective for lumbar vertebrae? J Dairy Sci 2025;108:3480-3498. [PMID: 39892604 DOI: 10.3168/jds.2024-25896] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 10/20/2024] [Accepted: 12/24/2024] [Indexed: 02/04/2025]

Abstract

Bone health is vital through all life stages and has become a growing public health concern. The role of rhizoma drynariae (RD) as a traditional Chinese medicine for bone injury recovery has been widely investigated. Still, there is little research related to RD as a functional component in food to improve calcium bioavailability and bone health. Milk powder is a daily vital food source of dietary calcium. The present study aimed to investigate and compare the effects of a functional milk powder reinforced with RD extracts (RDE) or vitamin D, vitamin K2, and calcium, or both, using Sprague-Dawley rats, with both low calcium growing rat model and low calcium ovariectomized (OVX) rat model. For growing rats, RDE increased bone mineral density (BMD) and improved the microstructure of bone trabecula in the femur and lumbar vertebrae. The levels of serum bone turnover markers C-telopeptide of type Ⅰ collagen (CTX-I), procollagen type Ⅰ amino-terminal peptide (PINP), and osteocalcin (BGP) were increased with RDE supplementation. The tartrate-resistant acid phosphatase staining suggested a decrease in the number of osteoclasts in the femur. Immunohistochemistry showed that the osteoclast-related protein RANKL was downregulated, and the osteogenic-associated proteins RUNX2 and OSX significantly increased in the lumbar vertebrae. Similarly, in OVX rats, RDE improved BMD and microstructure in lumbar vertebrae, and the levels of CTX-I, PINP, and BGP were also increased. In conclusion, the novel functional milk powder supplemented with RDE facilitated osteogenesis and inhibited osteoclast in rats at both stages, especially with lumbar vertebrae in growing rats. Our findings provide new prospects and a more precise target for enhancing bone health by incorporating RDE as a functional component in milk powder.

Collapse

Rossip M, Lorenz FJ, Sciscent B, Truong N, Nimmagadda C, Goyal N, Goldenberg D. Effect of Histamine-2 Receptor Antagonists on Hypocalcemia after Thyroidectomy: A Retrospective Cohort Study and Sensitivity Analysis. Otolaryngol Head Neck Surg 2025;172:1199-1207. [PMID: 39865431 PMCID: PMC11947861 DOI: 10.1002/ohn.1140] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Grants] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 10/14/2024] [Revised: 11/27/2024] [Accepted: 01/03/2025] [Indexed: 01/28/2025]

Balkrishna A, Sinha S, Shukla S, Bhattacharya K, Varshney A. Anti-ulcerogenic activity of the marine-pearl derived medicine mukta Pishti in Rat model of pylorus ligation-induced peptic ulcer. JOURNAL OF ETHNOPHARMACOLOGY 2025;342:119378. [PMID: 39828143 DOI: 10.1016/j.jep.2025.119378] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 10/30/2024] [Revised: 01/15/2025] [Accepted: 01/16/2025] [Indexed: 01/22/2025]

Deng R, Nau F, Lucas T, Maillard MB, Leduc A, Ossemond J, Musse M, Le Feunteun S. First assessments of nutrient bioaccessibility with an INFOGEST semi-dynamic gastric digestion in vitro protocol adapted to model proton pump inhibitor use. Food Res Int 2025;203:115833. [PMID: 40022357 DOI: 10.1016/j.foodres.2025.115833] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 07/21/2024] [Revised: 01/20/2025] [Accepted: 01/23/2025] [Indexed: 03/03/2025]

Abstract

Physiologically relevant gastrointestinal digestion models for infants, adults, and the elderly are commonly used to explore the fate of food in vitro. However, no consensus protocol exists to simulate the specific conditions observed in the stomach of people using oral proton pump inhibitors (PPIs), a class of widely prescribed medications that reduce gastric acid secretion and may alter food digestion and nutrient absorption. The first objective of this study was to propose an in vitro gastric digestion protocol adapted to model PPI use. This protocol is an extension of the semi-dynamic INFOGEST protocol previously developed to mimic the digestion of an adult human (referred to hereinafter as "Standard"), with two modifications based on reported clinical effects of PPIs: (i) a final gastric pH of 4.2 and (ii) a 50 % reduction in simulated gastric acid fluid volume. The second objective was to compare the release kinetics of peptides, soluble carbohydrates, lipids, and minerals during simulated gastric digestion of a mixed meal (bread, cheese, and tomato) with both standard and PPI versions of the protocol. Results demonstrated that the release of peptides, arabinose, and minerals, including calcium, magnesium, and phosphorus, was significantly (p < 0.05) reduced in the PPI model, while the hydrolyses of starch and lipids, assessed through maltose release and triacylglycerol disappearance, respectively, were not significantly affected. These findings are in agreement with the expected effects of reduced gastric acidity on pepsin activity and mineral solubility. These findings are also consistent with known or presumed side effects of PPIs such as an increased risk of hypomagnesemia, fractures, skeletal muscle loss, and vitamin B12 deficiency. In conclusion, this modified INFOGEST protocol appears to serve as a valuable tool to study the side effects of PPI use on food digestion and related nutrient bioaccessibility.

Collapse

Thonapan N, Wongdee K, Aksornthong S, Teerapornpuntakit J, Tiyasatkulkovit W, Panupinthu N, Charoenphandhu N. Long-term excessive salt consumption alters villous and crypt morphology and the protein expression of uroguanylin, TRPV6 and PMCA1b in the rat small intestine. PLoS One 2025;20:e0317415. [PMID: 39820616 PMCID: PMC11737712 DOI: 10.1371/journal.pone.0317415] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [MESH Headings] [Grants] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 07/31/2024] [Accepted: 12/27/2024] [Indexed: 01/19/2025] Open

Passman JE, Ginzberg S, Gasior JA, Krumeich L, Brensinger C, Bader A, Hwang J, Kelz R, Wachtel H. Gastrointestinal surgery, malabsorptive conditions, and postoperative hypocalcemia after neck surgery. Surgery 2025;177:108905. [PMID: 39472261 PMCID: PMC11649444 DOI: 10.1016/j.surg.2024.08.057] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Grants] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 04/26/2024] [Revised: 07/15/2024] [Accepted: 08/08/2024] [Indexed: 12/17/2024]

Abstract

BACKGROUND

Postoperative hypocalcemia is a common complication of thyroid and parathyroid surgery. Patients with prior gastric bypass face increased risk of postoperative hypocalcemia, but the impact of other malabsorptive conditions is not well understood. In this study, we evaluated the relationship between multiple medical and surgical malabsorptive states and hypocalcemia after thyroid and parathyroid surgery.

METHODS

We performed a retrospective cohort study of patients who underwent total thyroidectomy and/or parathyroidectomy in Optum's deidentified Clinformatics Data Mart Database (2004-2022). Patients were categorized as having surgical (foregut/midgut: gastrectomy, intestinal bypass, enterectomy, enterostomy, pancreatectomy, or hindgut: colectomy/colostomy) or medical (Crohn or Celiac disease) malabsorptive conditions. The primary outcomes were early (<7 days) and late (7-365 days) postoperative hypocalcemia. Logistic regression was performed to determine the associations between malabsorptive conditions and outcomes.

RESULTS

Of 25,400 patients (56.9% total thyroidectomy, 40.8% parathyroidectomy, and 2.4% both procedures), 4.0% had a pre-existing malabsorptive condition. Early postoperative hypocalcemia occurred in 8.8% of patients, and late hypocalcemia in 18.3%. Thyroidectomy was associated with a greater likelihood of hypocalcemia than parathyroidectomy (odds ratio: 1.22; P < .001). Pancreatectomy was associated with twice the adjusted odds of postoperative hypocalcemia (odds ratio: 2.27; P = .031) across both procedures. Patients with prior foregut/midgut surgery were at higher risk after total thyroidectomy (odds ratio: 1.65, P = .002). This association was significant in late (odds ratio: 1.82, P < .001) rather than early hypocalcemia (odds ratio: 1.33, P = .175). Hindgut surgery and medical malabsorption did not demonstrate such associations.

CONCLUSION

Prior foregut and midgut resections may predispose patients to postoperative hypocalcemia, particularly in patients undergoing total thyroidectomy.

Collapse

Lee HJ, Ahn HS, Han DS. Perioperative risk factors for osteoporosis after radical gastrectomy for gastric cancer. BMC Surg 2024;24:420. [PMID: 39731108 DOI: 10.1186/s12893-024-02717-4] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 09/04/2024] [Accepted: 12/10/2024] [Indexed: 12/29/2024] Open

Zhou H, Hu YY, Tang ZX, Jiang ZB, Huang J, Zhang T, Shen HY, Ye XP, Huang XY, Wang X, Zhou T, Bai XL, Zhu Q, Shi LE. Calcium Transport and Enrichment in Microorganisms: A Review. Foods 2024;13:3612. [PMID: 39594028 PMCID: PMC11593130 DOI: 10.3390/foods13223612] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Grants] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 08/30/2024] [Revised: 10/31/2024] [Accepted: 11/10/2024] [Indexed: 11/28/2024] Open

Affiliation(s)

Hai Zhou Department of Biotechnology, College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, China; (H.Z.); (Y.-Y.H.); (Z.-B.J.); (J.H.); (T.Z.); (H.-Y.S.); (X.-P.Y.); (X.-Y.H.); (X.W.); (T.Z.); (X.-L.B.); (Q.Z.)
Yan-Yu Hu Department of Biotechnology, College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, China; (H.Z.); (Y.-Y.H.); (Z.-B.J.); (J.H.); (T.Z.); (H.-Y.S.); (X.-P.Y.); (X.-Y.H.); (X.W.); (T.Z.); (X.-L.B.); (Q.Z.)
Zhen-Xing Tang School of Culinary Art, Tourism College of Zhejiang, Hangzhou 311231, China
Zhong-Bao Jiang Department of Biotechnology, College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, China; (H.Z.); (Y.-Y.H.); (Z.-B.J.); (J.H.); (T.Z.); (H.-Y.S.); (X.-P.Y.); (X.-Y.H.); (X.W.); (T.Z.); (X.-L.B.); (Q.Z.)
Jie Huang Department of Biotechnology, College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, China; (H.Z.); (Y.-Y.H.); (Z.-B.J.); (J.H.); (T.Z.); (H.-Y.S.); (X.-P.Y.); (X.-Y.H.); (X.W.); (T.Z.); (X.-L.B.); (Q.Z.)
Tian Zhang Department of Biotechnology, College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, China; (H.Z.); (Y.-Y.H.); (Z.-B.J.); (J.H.); (T.Z.); (H.-Y.S.); (X.-P.Y.); (X.-Y.H.); (X.W.); (T.Z.); (X.-L.B.); (Q.Z.)
Hui-Yang Shen Department of Biotechnology, College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, China; (H.Z.); (Y.-Y.H.); (Z.-B.J.); (J.H.); (T.Z.); (H.-Y.S.); (X.-P.Y.); (X.-Y.H.); (X.W.); (T.Z.); (X.-L.B.); (Q.Z.)
Xin-Pei Ye Department of Biotechnology, College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, China; (H.Z.); (Y.-Y.H.); (Z.-B.J.); (J.H.); (T.Z.); (H.-Y.S.); (X.-P.Y.); (X.-Y.H.); (X.W.); (T.Z.); (X.-L.B.); (Q.Z.)
Xuan-Ya Huang Department of Biotechnology, College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, China; (H.Z.); (Y.-Y.H.); (Z.-B.J.); (J.H.); (T.Z.); (H.-Y.S.); (X.-P.Y.); (X.-Y.H.); (X.W.); (T.Z.); (X.-L.B.); (Q.Z.)
Xiang Wang Department of Biotechnology, College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, China; (H.Z.); (Y.-Y.H.); (Z.-B.J.); (J.H.); (T.Z.); (H.-Y.S.); (X.-P.Y.); (X.-Y.H.); (X.W.); (T.Z.); (X.-L.B.); (Q.Z.)
Ting Zhou Department of Biotechnology, College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, China; (H.Z.); (Y.-Y.H.); (Z.-B.J.); (J.H.); (T.Z.); (H.-Y.S.); (X.-P.Y.); (X.-Y.H.); (X.W.); (T.Z.); (X.-L.B.); (Q.Z.)
Xue-Lian Bai Department of Biotechnology, College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, China; (H.Z.); (Y.-Y.H.); (Z.-B.J.); (J.H.); (T.Z.); (H.-Y.S.); (X.-P.Y.); (X.-Y.H.); (X.W.); (T.Z.); (X.-L.B.); (Q.Z.)
Qin Zhu Department of Biotechnology, College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, China; (H.Z.); (Y.-Y.H.); (Z.-B.J.); (J.H.); (T.Z.); (H.-Y.S.); (X.-P.Y.); (X.-Y.H.); (X.W.); (T.Z.); (X.-L.B.); (Q.Z.)
Lu-E Shi Department of Biotechnology, College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, China; (H.Z.); (Y.-Y.H.); (Z.-B.J.); (J.H.); (T.Z.); (H.-Y.S.); (X.-P.Y.); (X.-Y.H.); (X.W.); (T.Z.); (X.-L.B.); (Q.Z.)

Collapse

Duarte GJ, Lopez J, Sosa F, Molina G, Shaban M, Mark J, Khizar A, Sreenivasan A, Tran A, Guerra MR. Proton pump inhibitors and cardiovascular risk: a critical review. Future Cardiol 2024;20:779-794. [PMID: 39466134 PMCID: PMC11622795 DOI: 10.1080/14796678.2024.2412910] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 05/08/2024] [Accepted: 10/02/2024] [Indexed: 10/29/2024] Open

Wielogórska-Partyka M, Godzien J, Podgórska-Golubiewska B, Sieminska J, Mamani-Huanca M, Mocarska K, Stępniewska M, Supronik J, Pomichter B, Lopez-Gonzalvez A, Kozłowska G, Buczyńska A, Popławska-Kita A, Adamska A, Szelachowska M, Barbas C, Ciborowski M, Siewko K, Krętowski A. New insight into primary hyperparathyroidism using untargeted metabolomics. Sci Rep 2024;14:20987. [PMID: 39251672 PMCID: PMC11385525 DOI: 10.1038/s41598-024-71423-1] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 04/20/2024] [Accepted: 08/28/2024] [Indexed: 09/11/2024] Open

Affiliation(s)

Marta Wielogórska-Partyka Department of Endocrinology, Diabetology and Internal Medicine, Medical University of Bialystok, 15-276, Białystok, Poland
Joanna Godzien Metabolomics and Proteomics Laboratory, Clinical Research Centre, Medical University of Bialystok, Skłodowskiej 24a, 15-276, Białystok, Poland.
Beata Podgórska-Golubiewska Department of Endocrinology, Diabetology and Internal Medicine, Medical University of Bialystok, 15-276, Białystok, Poland
Julia Sieminska Metabolomics and Proteomics Laboratory, Clinical Research Centre, Medical University of Bialystok, Skłodowskiej 24a, 15-276, Białystok, Poland
Maricruz Mamani-Huanca Centro de Metabolómica y Bioanálisis (CEMBIO), Facultad de Farmacia, Universidad San Pablo-CEU, CEU Universities, Urbanización Montepríncipe, 28660, Boadilla del Monte, Spain
Karolina Mocarska Department of Endocrinology, Diabetology and Internal Medicine, Medical University of Bialystok, 15-276, Białystok, Poland
Marta Stępniewska Department of Endocrinology, Diabetology and Internal Medicine, Medical University of Bialystok, 15-276, Białystok, Poland
Jakub Supronik Department of Endocrinology, Diabetology and Internal Medicine, Medical University of Bialystok, 15-276, Białystok, Poland
Bartosz Pomichter Department of Endocrinology, Diabetology and Internal Medicine, Medical University of Bialystok, 15-276, Białystok, Poland
Angeles Lopez-Gonzalvez Centro de Metabolómica y Bioanálisis (CEMBIO), Facultad de Farmacia, Universidad San Pablo-CEU, CEU Universities, Urbanización Montepríncipe, 28660, Boadilla del Monte, Spain
Gabryela Kozłowska Clinical Research Centre, Medical University of Bialystok, 15-276, Białystok, Poland
Angelika Buczyńska Clinical Research Centre, Medical University of Bialystok, 15-276, Białystok, Poland
Anna Popławska-Kita Department of Endocrinology, Diabetology and Internal Medicine, Medical University of Bialystok, 15-276, Białystok, Poland
Agnieszka Adamska Department of Endocrinology, Diabetology and Internal Medicine, Medical University of Bialystok, 15-276, Białystok, Poland
Małgorzata Szelachowska Department of Endocrinology, Diabetology and Internal Medicine, Medical University of Bialystok, 15-276, Białystok, Poland
Coral Barbas Centro de Metabolómica y Bioanálisis (CEMBIO), Facultad de Farmacia, Universidad San Pablo-CEU, CEU Universities, Urbanización Montepríncipe, 28660, Boadilla del Monte, Spain
Michal Ciborowski Metabolomics and Proteomics Laboratory, Clinical Research Centre, Medical University of Bialystok, Skłodowskiej 24a, 15-276, Białystok, Poland
Katarzyna Siewko Department of Endocrinology, Diabetology and Internal Medicine, Medical University of Bialystok, 15-276, Białystok, Poland
Adam Krętowski Department of Endocrinology, Diabetology and Internal Medicine, Medical University of Bialystok, 15-276, Białystok, Poland Metabolomics and Proteomics Laboratory, Clinical Research Centre, Medical University of Bialystok, Skłodowskiej 24a, 15-276, Białystok, Poland Clinical Research Centre, Medical University of Bialystok, 15-276, Białystok, Poland

Collapse

Mei G, Wang J, Wang J, Ye L, Yi M, Chen G, Zhang Y, Tang Q, Chen L. The specificities, influencing factors, and medical implications of bone circadian rhythms. FASEB J 2024;38:e23758. [PMID: 38923594 DOI: 10.1096/fj.202302582rr] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Grants] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 12/13/2023] [Revised: 05/14/2024] [Accepted: 06/13/2024] [Indexed: 06/28/2024]

Affiliation(s)

Gang Mei Department of Stomatology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China School of Stomatology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China Hubei Province Key Laboratory of Oral and Maxillofacial Development and Regeneration, Wuhan, China
Jinyu Wang School of Stomatology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China Hubei Province Key Laboratory of Oral and Maxillofacial Development and Regeneration, Wuhan, China
Jiajia Wang Department of Stomatology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China School of Stomatology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China Hubei Province Key Laboratory of Oral and Maxillofacial Development and Regeneration, Wuhan, China
Lanxiang Ye Department of Stomatology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China School of Stomatology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China Hubei Province Key Laboratory of Oral and Maxillofacial Development and Regeneration, Wuhan, China
Ming Yi Department of Stomatology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China School of Stomatology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China Hubei Province Key Laboratory of Oral and Maxillofacial Development and Regeneration, Wuhan, China
Guangjin Chen Department of Stomatology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China School of Stomatology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China Hubei Province Key Laboratory of Oral and Maxillofacial Development and Regeneration, Wuhan, China
Yifan Zhang Department of Stomatology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China School of Stomatology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China Hubei Province Key Laboratory of Oral and Maxillofacial Development and Regeneration, Wuhan, China
Qingming Tang Department of Stomatology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China School of Stomatology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China Hubei Province Key Laboratory of Oral and Maxillofacial Development and Regeneration, Wuhan, China
Lili Chen Department of Stomatology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China School of Stomatology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China Hubei Province Key Laboratory of Oral and Maxillofacial Development and Regeneration, Wuhan, China

Collapse

Vavallo M, Cingolani S, Cozza G, Schiavone FP, Dottori L, Palumbo C, Lahner E. Autoimmune Gastritis and Hypochlorhydria: Known Concepts from a New Perspective. Int J Mol Sci 2024;25:6818. [PMID: 38999928 PMCID: PMC11241626 DOI: 10.3390/ijms25136818] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 05/12/2024] [Revised: 06/18/2024] [Accepted: 06/19/2024] [Indexed: 07/14/2024] Open

Zhang Y, Qiao Z, Yu J, Shi C, Quan R, Zhang W, Bi R, Li H, Qian W, Wang M, Li Y. Effects of Dietary Colostrum Basic Protein on Bone Growth and Calcium Absorption in Mice. Nutrients 2024;16:664. [PMID: 38474792 DOI: 10.3390/nu16050664] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Grants] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 11/22/2023] [Revised: 02/18/2024] [Accepted: 02/22/2024] [Indexed: 03/14/2024] Open

Zhang K, Gu F, Han Y, Cai T, Gu Z, Chen J, Chen B, Gao M, Hou Z, Yu X, Zhao J, Gao Y, Xie J, Hu R, Liu T, Li B. Association between dietary calcium intake and severe abdominal aorta calcification among American adults: a cross-sectional analysis of the National Health and Nutrition Examination Survey. Ther Adv Cardiovasc Dis 2024;18:17539447241232774. [PMID: 38415471 PMCID: PMC10903221 DOI: 10.1177/17539447241232774] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Received: 07/06/2023] [Accepted: 01/30/2024] [Indexed: 02/29/2024] Open

Paudel Y, Najam B, Desai HN, Illango J, Seffah KD, Kumar M, Naveen N, Pachchipulusu VK, Penumetcha SS. Use of Proton Pump Inhibitors and Risk of Fracture in Adults: A Review of Literature. Cureus 2023;15:e49872. [PMID: 38170137 PMCID: PMC10760937 DOI: 10.7759/cureus.49872] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 06/26/2023] [Accepted: 12/03/2023] [Indexed: 01/05/2024] Open

Fitzpatrick D, Lannon R, Laird E, Ward M, Hoey L, Hughes CF, Strain JJ, Cunningham C, McNulty H, Molloy AM, McCarroll K. The association between proton pump inhibitors and hyperparathyroidism: a potential mechanism for increased fracture-results of a large observational cohort study. Osteoporos Int 2023;34:1917-1926. [PMID: 37530847 PMCID: PMC10579148 DOI: 10.1007/s00198-023-06867-8] [Citation(s) in RCA: 11] [Impact Index Per Article: 5.5] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Grants] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Received: 03/20/2023] [Accepted: 07/19/2023] [Indexed: 08/03/2023]

Abstract

Proton pump inhibitors (PPIs) are associated with increased risk of osteoporotic fracture; however, the mechanism is unclear. PPI users taking calcium supplements were more likely to have hyperparathyroidism compared to non-users (OR 1.56, CI 1.08-2.23, p = 0.018). This highlights the importance of monitoring PPI use, especially in older adults.

PURPOSE

Proton pump inhibitors (PPIs) are associated with increased risk of osteoporotic fracture. Hyperparathyroidism may be implicated, but few studies have considered this relationship. This study evaluated the relationship between PPI use and hyperparathyroidism in older adults.

METHODS

Participants were from the TUDA study, a large cross-sectional cohort of older Irish adults. Participants with an estimated glomerular filtration rate (eGFR) < 30 ml/min and serum calcium > 2.5 mmol/l were excluded to avoid hyperparathyroidism due to chronic renal disease and primary hyperparathyroidism. Hyperparathyroidism was defined as a parathyroid hormone (PTH) > 65 pg/ml. Multivariate regression models were used to analyse the relationship between PPI use and hyperparathyroidism.

RESULTS

A total of 4139 participants met the inclusion criteria, of whom 37.8% (n = 1563) were taking PPI medication. PPI use was identified in 41.4% of calcium supplement users and 35.4% of non-calcium supplement users. Overall, compared to non-users of PPIs, those taking PPIs were older (74.8 vs 72.9 years, p < 0.001) and had a higher prevalence of hyperparathyroidism (17.8 vs 11.0%, p < 0.001). In those taking calcium supplements (but not in non-users), PPI use was significantly associated with hyperparathyroidism (OR 1.56, CI 1.08-2.23, p = 0.018) after adjusting for age, sex, body mass index, serum vitamin D, eGFR, timed-up-and-go, dairy intake, medications, and comorbidities.

DISCUSSION

The results are consistent with the hypothesis of PPIs reducing calcium absorption, leading to a rise in PTH which could mediate increased fracture risk. No relationship of PPI use with hyperparathyroidism was observed in non-users of calcium supplements, possibly owing to lower dietary calcium intake. These results highlight the importance of monitoring PPI use, especially in older adults at risk of fracture.

Collapse

Liao Z, Liu F, Wang Y, Fan X, Li Y, He J, Buttino I, Yan X, Zhang X, Shi G. Transcriptomic response of Mytilus coruscus mantle to acute sea water acidification and shell damage. Front Physiol 2023;14:1289655. [PMID: 37954445 PMCID: PMC10639161 DOI: 10.3389/fphys.2023.1289655] [Citation(s) in RCA: 2] [Impact Index Per Article: 1.0] [Reference Citation Analysis] [Abstract] [Key Words] [Grants] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 09/06/2023] [Accepted: 10/16/2023] [Indexed: 11/14/2023] Open

Abstract

Mytilus coruscus is an economically important marine calcifier living in the Yangtze River estuary sea area, where seasonal fluctuations in natural pH occur owing to freshwater input, resulting in a rapid reduction in seawater pH. In addition, Mytilus constantly suffers from shell fracture or injury in the natural environment, and the shell repair mechanisms in mussels have evolved to counteract shell injury. Therefore, we utilized shell-complete and shell-damaged Mytilus coruscus in this study and performed transcriptomic analysis of the mantle to investigate whether the expression of mantle-specific genes can be induced by acute seawater acidification and how the mantle responds to acute acidification during the shell repair process. We found that acute acidification induced more differentially expressed genes than shell damage in the mantle, and the biomineralization-related Gene Ontology terms and KEGG pathways were significantly enriched by these DEGs. Most DEGs were upregulated in enriched pathways, indicating the activation of biomineralization-related processes in the mussel mantle under acute acidification. The expression levels of some shell matrix proteins and antimicrobial peptides increased under acute acidification and/or shell damage, suggesting the molecular modulation of the mantle for the preparation and activation of the shell repairing and anti-infection under adverse environmental conditions. In addition, morphological and microstructural analyses were performed for the mantle edge and shell cross-section, and changes in the mantle secretory capacity and shell inner film system induced by the two stressors were observed. Our findings highlight the adaptation of M. coruscus in estuarine areas with dramatic fluctuations in pH and may prove instrumental in its ability to survive ocean acidification.

Collapse

Saki F, Shams M, Dastghaib S, Koohpeyma F. Pantoprazole-Induced Bone Loss through Gastrin Secretion: A Stereological Study. BIOMED RESEARCH INTERNATIONAL 2023;2023:2594664. [PMID: 37711876 PMCID: PMC10499535 DOI: 10.1155/2023/2594664] [Citation(s) in RCA: 3] [Impact Index Per Article: 1.5] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Figures] [Subscribe] [Scholar Register] [Received: 02/01/2023] [Revised: 04/20/2023] [Accepted: 07/11/2023] [Indexed: 09/16/2023]

Li G, Zhang J, Xu D, Cao Y. Structural changes and calcium bioaccessibility of calcium fortified milk containing CaCO₃ loaded solid-in-oil-in-water emulsion during simulated in vitro digestion. JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE 2023;103:5862-5872. [PMID: 37093558 DOI: 10.1002/jsfa.12657] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Grants] [Track Full Text] [Subscribe] [Scholar Register] [Received: 10/07/2022] [Revised: 04/11/2023] [Accepted: 04/24/2023] [Indexed: 05/03/2023]

Zhou W, Duan T. Effects of maternal calcium and protein intake on the development and bone metabolism of offspring mice. Open Life Sci 2023;18:20220631. [PMID: 37426616 PMCID: PMC10329277 DOI: 10.1515/biol-2022-0631] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 02/06/2023] [Revised: 04/19/2023] [Accepted: 05/17/2023] [Indexed: 07/11/2023] Open

Arboleya L, Braña I, Pardo E, Loredo M, Queiro R. Osteomalacia in Adults: A Practical Insight for Clinicians. J Clin Med 2023;12:jcm12072714. [PMID: 37048797 PMCID: PMC10094844 DOI: 10.3390/jcm12072714] [Citation(s) in RCA: 3] [Impact Index Per Article: 1.5] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 02/26/2023] [Revised: 03/26/2023] [Accepted: 04/01/2023] [Indexed: 04/14/2023] Open

Jiang S, Zou X, Mao M, Zhang M, Tu W, Jin M. Low Ca diet leads to increased Ca retention by changing the gut flora and ileal pH value in laying hens. ANIMAL NUTRITION 2023;13:270-281. [PMID: 37168452 PMCID: PMC10164782 DOI: 10.1016/j.aninu.2023.02.006] [Citation(s) in RCA: 6] [Impact Index Per Article: 3.0] [Reference Citation Analysis] [Abstract] [Track Full Text] [Subscribe] [Scholar Register] [Received: 04/07/2022] [Revised: 01/16/2023] [Accepted: 02/09/2023] [Indexed: 02/27/2023]

Abstract

Osteoporosis is a common degenerative metabolic bone disease in caged laying hens. Intensive egg production mobilizing large amounts of Ca from bone for eggshell formation, consequently leading to Ca deficiency, has been recognized as a critical factor causing osteoporosis in commercial laying hens. The aim of this study was to examine the effect of Ca deficiency on the function of the gut microbiota-bone axis and related egg production traits and bone health in laying hens. Twenty-four 48-week-old laying hens were fed a control diet (Control, 3.72%) or a low Ca diet (LC, 2.04%) for 60 d (n = 12). Compared to the Control hens, the LC hens had higher levels of alkaline phosphatase and tartrate resistant acid phosphatase (P < 0.05) with lower bone strength, eggshell thickness, and eggshell strength (P < 0.05). In addition, the LC hens had higher plasma estradiol concentrations, while having lower concentrations of interleukin-1 (IL-1) and IL-6. The LC hens also had a lower pH value in the ileum with an increased Ca retention. The principal co-ordinates analysis showed significantly separate cecal microbiota populations between the Control and LC hens. The Prevotellaceae_UCG-001, Subdoligranulum, Peptococcus, and Eubacterium_hallii_group (P < 0.05) were higher, while the CHKC1001 and Sutterella (P < 0.05) were lower at the genus level in the LC hens. In addition, Prevotellaceae_UCG-001, Subdoligranulum and Eubacterium_hallii_group had a negative correlation, while Sutterella was positively correlated with ileal pH values. The transcriptome analysis revealed that the low Ca diet caused 20 and 31 genes to be significantly up- and down-regulated, respectively. The gene expressions of cystic fibrosis transmembrane conductance regulator, solute carrier family 26 member 3 of the anion exchangers, and mitogen-activated protein kinase 12 of pro-inflammatory factors were lower in the LC birds, which was correlated with the lower ileal pH values. These results suggest that the hens with low Ca diet-induced osteoporosis have an increased intestinal Ca retention with a decreased ileal pH value, correlated with the changes in Prevotellaceae_UCG-001, Subdoligranulum, and Eubacterium_hallii_group of beneficial genera. The results provide insights for further understanding and preventing osteoporosis in laying hens.

Collapse

Aenglong C, Ngasakul N, Limpawattana M, Sukketsiri W, Chockchaisawasdee S, Stathopoulos C, Tanasawet S, Klaypradit W. Characterization of novel calcium compounds from tilapia (Oreochromis niloticus) by-products and their effects on proliferation and differentiation of MC3T3-E1 cells. J Funct Foods 2023. [DOI: 10.1016/j.jff.2022.105361] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 12/12/2022] Open

Rodrat M, Wongdee K, Teerapornpuntakit J, Thongbunchoo J, Tanramluk D, Aeimlapa R, Thammayon N, Thonapan N, Wattano P, Charoenphandhu N. Vasoactive intestinal peptide and cystic fibrosis transmembrane conductance regulator contribute to the transepithelial calcium transport across intestinal epithelium-like Caco-2 monolayer. PLoS One 2022;17:e0277096. [PMID: 36399482 PMCID: PMC9674163 DOI: 10.1371/journal.pone.0277096] [Citation(s) in RCA: 3] [Impact Index Per Article: 1.0] [Reference Citation Analysis] [Abstract] [MESH Headings] [Grants] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 08/12/2022] [Accepted: 10/20/2022] [Indexed: 11/19/2022] Open

Abstract

Vasoactive intestinal peptide (VIP) as a neurocrine factor released by enteric neurons has been postulated to participate in the regulation of transcellular active calcium transport across intestinal epithelium, but the preceding evidence is scant and inconclusive. Herein, transepithelial calcium flux and epithelial electrical parameters were determined by Ussing chamber technique with radioactive tracer in the intestinal epithelium-like Caco-2 monolayer grown on Snapwell. After 3-day culture, Caco-2 cells expressed mRNA of calcium transporters, i.e., TRPV6, calbindin-D9k, PMCA1b and NCX1, and exhibited transepithelial resistance of ~200 Ω cm2, a characteristic of leaky epithelium similar to the small intestine. VIP receptor agonist was able to enhance transcellular calcium flux, whereas VIP receptor antagonist totally abolished calcium fluxes induced by 1,25-dihydroxyvitamin D3 [1,25(OH)2D3]. Since the intestinal cystic fibrosis transmembrane conductance regulator (CFTR) could be activated by VIP and calciotropic hormones, particularly parathyroid hormone, we sought to determine whether CFTR also contributed to the 1,25(OH)2D3-induced calcium transport. A selective CFTR inhibitor (20-200 μM CFTRinh-172) appeared to diminish calcium fluxes as well as transepithelial potential difference and short-circuit current, both of which indicated a decrease in electrogenic ion transport. On the other hand, 50 μM genistein-a molecule that could rapidly activate CFTR-was found to increase calcium transport. Our in silico molecular docking analysis confirmed direct binding of CFTRinh-172 and genistein to CFTR channels. In conclusion, VIP and CFTR apparently contributed to the intestinal calcium transport, especially in the presence of 1,25(OH)2D3, thereby supporting the existence of the neurocrine control of intestinal calcium absorption.

Collapse

Affiliation(s)

Mayuree Rodrat Faculty of Science, Center of Calcium and Bone Research (COCAB), Mahidol University, Bangkok, Thailand Faculty of Science, Department of Physiology, Mahidol University, Bangkok, Thailand Institute of Molecular Biosciences, Mahidol University, Nakhon Pathom, Thailand Center of Research and Development for Biomedical Instrumentation, Institute of Molecular Biosciences, Mahidol University, Nakhon Pathom, Thailand
Kannikar Wongdee Faculty of Science, Center of Calcium and Bone Research (COCAB), Mahidol University, Bangkok, Thailand Faculty of Allied Health Sciences, Burapha University, Chonburi, Thailand
Jarinthorn Teerapornpuntakit Faculty of Science, Center of Calcium and Bone Research (COCAB), Mahidol University, Bangkok, Thailand Department of Physiology, Faculty of Medical Science, Naresuan University, Phitsanulok, Thailand
Jirawan Thongbunchoo Faculty of Science, Center of Calcium and Bone Research (COCAB), Mahidol University, Bangkok, Thailand Faculty of Science, Department of Physiology, Mahidol University, Bangkok, Thailand
Duangrudee Tanramluk Institute of Molecular Biosciences, Mahidol University, Nakhon Pathom, Thailand Integrative Computational BioScience (ICBS) Center, Mahidol University, Nakhon Pathom, Thailand
Ratchaneevan Aeimlapa Faculty of Science, Center of Calcium and Bone Research (COCAB), Mahidol University, Bangkok, Thailand Faculty of Science, Department of Physiology, Mahidol University, Bangkok, Thailand
Nithipak Thammayon Faculty of Science, Center of Calcium and Bone Research (COCAB), Mahidol University, Bangkok, Thailand Faculty of Science, Graduate Program in Molecular Medicine, Mahidol University, Bangkok, Thailand
Natchayaporn Thonapan Faculty of Science, Center of Calcium and Bone Research (COCAB), Mahidol University, Bangkok, Thailand Faculty of Science, Graduate Program in Molecular Medicine, Mahidol University, Bangkok, Thailand
Pathnaree Wattano Faculty of Science, Center of Calcium and Bone Research (COCAB), Mahidol University, Bangkok, Thailand
Narattaphol Charoenphandhu Faculty of Science, Center of Calcium and Bone Research (COCAB), Mahidol University, Bangkok, Thailand Faculty of Science, Department of Physiology, Mahidol University, Bangkok, Thailand Institute of Molecular Biosciences, Mahidol University, Nakhon Pathom, Thailand The Academy of Science, The Royal Society of Thailand, Bangkok, Thailand

Collapse

Wang J, Fan J, Yang Y, Moazzen S, Chen D, Sun L, He F, Li Y. Vitamin D Status and Risk of All-Cause and Cause-Specific Mortality in Osteoarthritis Patients: Results from NHANES III and NHANES 2001-2018. Nutrients 2022;14:nu14214629. [PMID: 36364891 PMCID: PMC9655488 DOI: 10.3390/nu14214629] [Citation(s) in RCA: 14] [Impact Index Per Article: 4.7] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 10/19/2022] [Revised: 10/27/2022] [Accepted: 10/30/2022] [Indexed: 11/06/2022] Open

Wang Z, Ma R, Jia Z, Lin P, Zhao Z, Wang W, Yi S, Li X, Li J. Investigating on the influence mechanism of sausage of sea bass on calcium absorption and transport based on Caco-2 cell monolayer model. Front Nutr 2022;9:1046945. [PMID: 36330132 PMCID: PMC9623112 DOI: 10.3389/fnut.2022.1046945] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 09/17/2022] [Accepted: 10/03/2022] [Indexed: 11/25/2022] Open

Changes in bone turnover markers in adolescents with gastroesophageal reflux disease treated with lansoprazole. Acta Gastroenterol Belg 2022;85:565-571. [PMID: 36566365 DOI: 10.51821/85.4.10906] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Indexed: 12/26/2022]

Enhancing the Dispersion Stability and Sustained Release of S/O/W Emulsions by Encapsulation of CaCO3 Droplets in Sodium Caseinate/Xanthan Gum Microparticles. Foods 2022;11:foods11182854. [PMID: 36140982 PMCID: PMC9498002 DOI: 10.3390/foods11182854] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 08/12/2022] [Revised: 09/05/2022] [Accepted: 09/08/2022] [Indexed: 12/05/2022] Open

Mori Y, Omori M, Nakao A. Vital but vulnerable: Human TRPV6 is a trade-off of powerful Ca²⁺ uptake and susceptibility to epithelial barrier dysfunction. Cell Calcium 2022;107:102652. [PMID: 36162385 DOI: 10.1016/j.ceca.2022.102652] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 09/04/2022] [Revised: 09/08/2022] [Accepted: 09/11/2022] [Indexed: 11/17/2022]

Phummisutthigoon S, Lertsuwan K, Panupinthu N, Aeimlapa R, Teerapornpuntakit J, Chankamngoen W, Thongbunchoo J, Charoenphandhu N, Wongdee K. Fe3+ opposes the 1,25(OH)2D3-induced calcium transport across intestinal epithelium-like Caco-2 monolayer in the presence or absence of ascorbic acid. PLoS One 2022;17:e0273267. [PMID: 36040915 PMCID: PMC9426938 DOI: 10.1371/journal.pone.0273267] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.3] [Reference Citation Analysis] [Abstract] [MESH Headings] [Grants] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 04/20/2022] [Accepted: 08/05/2022] [Indexed: 01/01/2023] Open

Affiliation(s)

Sukpapohn Phummisutthigoon Department of Physiology, Faculty of Science, Mahidol University, Bangkok, Thailand Center of Calcium and Bone Research (COCAB), Faculty of Science, Mahidol University, Bangkok, Thailand
Kornkamon Lertsuwan Center of Calcium and Bone Research (COCAB), Faculty of Science, Mahidol University, Bangkok, Thailand Department of Biochemistry, Faculty of Science, Mahidol University, Bangkok, Thailand
Nattapon Panupinthu Department of Physiology, Faculty of Science, Mahidol University, Bangkok, Thailand Center of Calcium and Bone Research (COCAB), Faculty of Science, Mahidol University, Bangkok, Thailand
Ratchaneevan Aeimlapa Department of Physiology, Faculty of Science, Mahidol University, Bangkok, Thailand Center of Calcium and Bone Research (COCAB), Faculty of Science, Mahidol University, Bangkok, Thailand
Jarinthorn Teerapornpuntakit Center of Calcium and Bone Research (COCAB), Faculty of Science, Mahidol University, Bangkok, Thailand Department of Physiology, Faculty of Medical Science, Naresuan University, Phitsanulok, Thailand
Wasutorn Chankamngoen Center of Calcium and Bone Research (COCAB), Faculty of Science, Mahidol University, Bangkok, Thailand Graduate Program in Molecular Medicine, Faculty of Science, Mahidol University, Bangkok, Thailand
Jirawan Thongbunchoo Department of Physiology, Faculty of Science, Mahidol University, Bangkok, Thailand Center of Calcium and Bone Research (COCAB), Faculty of Science, Mahidol University, Bangkok, Thailand
Narattaphol Charoenphandhu Department of Physiology, Faculty of Science, Mahidol University, Bangkok, Thailand Center of Calcium and Bone Research (COCAB), Faculty of Science, Mahidol University, Bangkok, Thailand Institute of Molecular Biosciences, Mahidol University, Nakhon Pathom, Thailand The Academy of Science, The Royal Society of Thailand, Dusit, Bangkok, Thailand
Kannikar Wongdee Center of Calcium and Bone Research (COCAB), Faculty of Science, Mahidol University, Bangkok, Thailand Faculty of Allied Health Sciences, Burapha University, Chonburi, Thailand

Collapse

Li G, Zhao Y, Zhang J, Hao J, Xu D, Cao Y. CaCO3 loaded lipid microspheres prepared by the solid-in-oil-in-water emulsions technique with propylene glycol alginate and xanthan gum. Front Nutr 2022;9:961326. [PMID: 36071930 PMCID: PMC9441954 DOI: 10.3389/fnut.2022.961326] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Grants] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 06/04/2022] [Accepted: 08/02/2022] [Indexed: 11/13/2022] Open

Arfuso F, Assenza A, Tosto F, Giannetto C, Interlandi C, Piccione G, Liotta L. Serum bone metabolism biomarkers in healthy filies and colts from weaning until one year of age. Res Vet Sci 2022;150:156-163. [PMID: 35841724 DOI: 10.1016/j.rvsc.2022.06.018] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 09/23/2021] [Revised: 04/06/2022] [Accepted: 06/21/2022] [Indexed: 10/17/2022]

Wijayanti I, Benjakul S, Saetang J, Prodpran T, Sookchoo P. Soluble Asian sea bass bone bio‐calcium: characteristics, bioavailability across Caco‐2 cells and fortification into apple juice. Int J Food Sci Technol 2022. [DOI: 10.1111/ijfs.15904] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.3] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 01/07/2023]

Gao J, Mei H, Sun J, Li H, Huang Y, Tang Y, Duan L, Liu D, Pang Y, Wang Q, Gao Y, Song K, Zhao J, Zhang C, Liu J. Neuropilin-1-Mediated SARS-CoV-2 Infection in Bone Marrow-Derived Macrophages Inhibits Osteoclast Differentiation. Adv Biol (Weinh) 2022;6:e2200007. [PMID: 35195371 PMCID: PMC9073998 DOI: 10.1002/adbi.202200007] [Citation(s) in RCA: 13] [Impact Index Per Article: 4.3] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 01/13/2022] [Revised: 01/25/2022] [Indexed: 01/27/2023]

Affiliation(s)

Junjie Gao Department of OrthopaedicsShanghai Jiao Tong University Affiliated Shanghai Sixth People's HospitalShanghai200233China
Hong Mei Shanghai Institute for Advanced Immunochemical Studies and School of Life Science and TechnologyShanghaiTech UniversityShanghai201210China
Jing Sun State Key Laboratory of Respiratory DiseaseNational Clinical Research Center for Respiratory DiseaseGuangzhou Institute of Respiratory Health, the First Affiliated Hospital of Guangzhou Medical UniversityGuangzhouGuangdong510182China
Hao Li Department of OrthopaedicsShanghai Jiao Tong University Affiliated Shanghai Sixth People's HospitalShanghai200233China
Yuege Huang Shanghai Institute for Advanced Immunochemical Studies and School of Life Science and TechnologyShanghaiTech UniversityShanghai201210China,Shanghai Clinical Research and Trial CenterShanghai201210China,Gene Editing CenterSchool of Life Science and TechnologyShanghaiTech UniversityShanghai201210China,University of Chinese Academy of SciencesBeijing100049China
Yanhong Tang State Key Laboratory of Respiratory DiseaseNational Clinical Research Center for Respiratory DiseaseGuangzhou Institute of Respiratory Health, the First Affiliated Hospital of Guangzhou Medical UniversityGuangzhouGuangdong510182China
Linwei Duan State Key Laboratory of Respiratory DiseaseNational Clinical Research Center for Respiratory DiseaseGuangzhou Institute of Respiratory Health, the First Affiliated Hospital of Guangzhou Medical UniversityGuangzhouGuangdong510182China
Delin Liu Centre for Orthopaedic ResearchSchool of SurgeryThe University of Western AustraliaNedlandsWestern Australia6009Australia
Yidan Pang Department of OrthopaedicsShanghai Jiao Tong University Affiliated Shanghai Sixth People's HospitalShanghai200233China
Qiyang Wang Department of OrthopaedicsShanghai Jiao Tong University Affiliated Shanghai Sixth People's HospitalShanghai200233China
Youshui Gao Department of OrthopaedicsShanghai Jiao Tong University Affiliated Shanghai Sixth People's HospitalShanghai200233China
Ke Song Shanghai Institute for Advanced Immunochemical Studies and School of Life Science and TechnologyShanghaiTech UniversityShanghai201210China
Jincun Zhao State Key Laboratory of Respiratory DiseaseNational Clinical Research Center for Respiratory DiseaseGuangzhou Institute of Respiratory Health, the First Affiliated Hospital of Guangzhou Medical UniversityGuangzhouGuangdong510182China
Changqing Zhang Department of OrthopaedicsShanghai Jiao Tong University Affiliated Shanghai Sixth People's HospitalShanghai200233China
Jia Liu Shanghai Institute for Advanced Immunochemical Studies and School of Life Science and TechnologyShanghaiTech UniversityShanghai201210China,Shanghai Clinical Research and Trial CenterShanghai201210China,Gene Editing CenterSchool of Life Science and TechnologyShanghaiTech UniversityShanghai201210China

Collapse

Esposito G, Dottori L, Pivetta G, Ligato I, Dilaghi E, Lahner E. Pernicious Anemia: The Hematological Presentation of a Multifaceted Disorder Caused by Cobalamin Deficiency. Nutrients 2022;14:nu14081672. [PMID: 35458234 PMCID: PMC9030741 DOI: 10.3390/nu14081672] [Citation(s) in RCA: 24] [Impact Index Per Article: 8.0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 03/08/2022] [Revised: 04/08/2022] [Accepted: 04/14/2022] [Indexed: 02/04/2023] Open

Wan Y, Chen X. Preparation and characterization of self-suspended CaCO3 nanoparticles derived from scallop shells. J DISPER SCI TECHNOL 2022. [DOI: 10.1080/01932691.2022.2028632] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 10/19/2022]

Matikainen N, Pekkarinen T, Ryhänen EM, Schalin-Jäntti C. Physiology of Calcium Homeostasis: An Overview. Endocrinol Metab Clin North Am 2021;50:575-590. [PMID: 34774235 DOI: 10.1016/j.ecl.2021.07.005] [Citation(s) in RCA: 71] [Impact Index Per Article: 17.8] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 12/29/2022]

QiangGuYin Modulates the OPG/RANKL/RANK Pathway by Increasing Secretin Levels during Treatment of Primary Type I Osteoporosis. EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE 2021;2021:7114139. [PMID: 34754319 PMCID: PMC8572595 DOI: 10.1155/2021/7114139] [Citation(s) in RCA: 3] [Impact Index Per Article: 0.8] [Reference Citation Analysis] [Abstract] [Track Full Text] [Download PDF] [Figures] [Subscribe] [Scholar Register] [Received: 05/15/2021] [Revised: 10/06/2021] [Accepted: 10/15/2021] [Indexed: 11/17/2022]

Abstract

QiangGuYin (QGY) is a common Traditional Chinese medicine prescription for the treatment of osteoporosis. Previous clinical studies have found that QGY effectively improves bone mineral density (BMD) in postmenopausal women, but its underlying mechanism remains unclear. The osteoprotegerin (OPG)/receptor activator of nuclear factor kappa B ligand (RANKL)/receptor activator of nuclear factor kappa B (RANK) pathway is a classic pathway involved in osteoporosis. Secretin levels are a serum marker of osteoporosis, but their effect on the OPG/RANKL/RANK pathway has not been reported. Hence, we investigated the relationship between the OPG/RANKL/RANK pathway and secretin and further revealed the mechanism underlying the effect of QGY in the treatment of osteoporosis. Mice were divided into secretin knockdown, secretin overexpression, and corresponding control groups. Micro-computed tomography was used to detect BMD in different groups, and the results show that QGY significantly improved BMD in mice of the secretin knockdown group. To further verify this, the serum levels of OPG, RANKL, RANK, and secretin were measured by enzyme-linked immunosorbent assays, and femur levels of OPG, RANKL, RANK, and secretin were evaluated by real-time quantitative PCR and western blotting. The results show that the expression of OPG was inhibited and that of RANKL and RANK was increased in mice from the secretin knockdown group, whereas the expression of OPG was upregulated and that of RANKL and RANK was downregulated after QGY intervention. Therefore, QGY inhibited bone resorption by promoting the expression of secretin and modulating the OPG/RANKL/RANK pathway. In addition to the effect of QGY, we also revealed the general regulatory effect of secretin on the OPG/RANKL/RANK pathway. We conclude that QGY modulates the OPG/RANKL/RANK pathway by increasing secretin levels during treatment of primary type I osteoporosis. This work provides a theoretical basis for the clinical use of QGY in the treatment of osteoporosis.

Collapse

Dotsenko E, Paliy А, Morozenko D, Dotsenko R, Zemlianskyi А, Pavlichenko O. Dose-dependent effect of chronic exposure to lead acetate on the dynamics of the content of delta-aminolevulinic acid and essential trace elements in the serum of laying hens. REGULATORY MECHANISMS IN BIOSYSTEMS 2021. [DOI: 10.15421/022195] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/05/2022] Open

Abstract One of the most global and dangerous pollutants is considered to be lead, due to its ability to accumulate in living organisms, to be included in the metabolic cycle, to form highly toxic organometallic compounds, not being biodegradable. To study the chronic effects of lead acetate, an experiment was performed on laying hens of Lohmann Brown cross. For research, one control and three experimental groups were formed, 20 chickens in each. Birds of the experimental groups were administered lead acetate daily with compound feed in doses (in terms of metal): Group I – 50 mg/kg of feed, II – 75 mg/kg of feed, III – 150 mg/kg of feed. Access to water was not restricted. The birds of the control group received a complete diet without lead acetate. On the 30th, 90th, 120th days of the experiment and the 20th day after withdrawal of the toxicant (the 140th day of the experiment), we took blood samples from birds for biochemical and toxicological studies. The concentration of delta-aminolevulinic acid (δ-ALA) in the serum of laying hens was determined by reversed-phase high-performance liquid chromatography. Determination of the content of lead in the serum of laying hens was performed by X-ray fluorescence analysis. The concentration of total calcium, inorganic phosphorus, and magnesium in the serum of the laying hens was determined using production kits. We detected the dose-dependent chronic effect of lead acetate on the dynamics of the content of delta-aminolevulinic acid and essential trace elements in the serum of laying hens. The administration of lead acetate into the body of productive poultry for 120 days in doses of 50, 75, and 150 mg/kg with feed did not cause visible clinical signs of poisoning, but caused a violation of porphyrin metabolism, manifested in a reliable increase in the concentration of delta-aminolevulinic acid in serum on the 30th, 90th and 120th days of the experiment. It has been experimentally proven that the administration into the body of laying hens of lead acetate in doses of 50, 75 and 150 mg/kg of feed for 120 days leads to the maximum accumulation of lead in the serum on the 30th, 90th, and 120th days of the experiment. Prolonged administration of lead acetate to laying hens at high concentrations is characterized by a reliable decrease in the content of total calcium, inorganic phosphorus and magnesium in the serum of laying hens and a reliable increase in the content of ferrum. In the future it is necessary to study the intensity of lipid peroxidation processes in laying hens under chronic exposure to lead acetate. Collapse

Zhang J, Li G, Xu D, Cao Y. Stability, Microstructure, and Rheological Properties of CaCO₃ S/O/W Calcium-Lipid Emulsions. Foods 2021;10:foods10092216. [PMID: 34574326 PMCID: PMC8468493 DOI: 10.3390/foods10092216] [Citation(s) in RCA: 2] [Impact Index Per Article: 0.5] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 06/23/2021] [Revised: 08/15/2021] [Accepted: 09/07/2021] [Indexed: 01/28/2023] Open

Abstract

Calcium carbonate (CaCO₃) is a commonly used fortified calcium, but poor suspension stability and easy precipitation seriously limited its food processing and products application. The formation of CaCO₃ loaded microparticles based on the form of solid/oil/water (S/O/W) emulsion is a promising method to improve the dispersion stability of CaCO₃ in liquid food. In this study, CaCO₃, soybean oil, and sodium caseinate (NaCas) were used as the solid, oil, and W phase, respectively. The fabrication involved two steps: the S/O emulsion was prepared by adding CaCO₃ into soybean oil by magnetic stirring and high-speed shearing, and then put the S/O crude emulsion into NaCas solution (W phase) to obtain S/O/W emulsion by high-speed blender. The particle size distribution, zeta potential, stability of the microsphere, infrared spectral analysis, and XRD of the S/O/W calcium-lipid microsphere were explored. The stability and rheological mechanism of S/O/W calcium-lipid emulsion were investigated by combining the microstructure, shear rheological, and microrheological properties. It was found that the emulsion particles have more uniform particle size distribution and no aggregation, and the stability of the emulsion was improved with increasing the content of NaCas. The mean square displacement (MSD) curve and solid-liquid equilibrium (SLB) value of S/O/W emulsion increased with the increase in NaCas concentration, and the viscosity behavior is dominant. The results of confocal laser microscopy (CLSM) and cryo-scanning electron microscopy (Cryo-SEM) showed that the three-dimensional network structure of S/O/W emulsions was more compact, and the embedding effect of calcium carbonate (CaCO₃) was slightly improved with the increase in NaCas concentration. According to infrared spectrum and XDR analysis, the addition of CaCO₃ into the emulsion system caused crystal structure distortion. This study provides a reference for solving the dispersibility of insoluble calcium salt in liquid food.

Collapse

Vanuytsel T, Tack J, Farre R. The Role of Intestinal Permeability in Gastrointestinal Disorders and Current Methods of Evaluation. Front Nutr 2021;8:717925. [PMID: 34513903 PMCID: PMC8427160 DOI: 10.3389/fnut.2021.717925] [Citation(s) in RCA: 129] [Impact Index Per Article: 32.3] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 05/31/2021] [Accepted: 08/04/2021] [Indexed: 12/12/2022] Open

Mulet-Cabero AI, Wilde PJ. Role of calcium on lipid digestion and serum lipids: a review. Crit Rev Food Sci Nutr 2021;63:813-826. [PMID: 34281429 DOI: 10.1080/10408398.2021.1954873] [Citation(s) in RCA: 19] [Impact Index Per Article: 4.8] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 02/08/2023]

Gifford AH, Sanville JL, Sathe M, Heltshe SL, Goss CH. Use of proton pump inhibitors is associated with lower hemoglobin levels in people with cystic fibrosis. Pediatr Pulmonol 2021;56:2048-2056. [PMID: 33860641 PMCID: PMC8217294 DOI: 10.1002/ppul.25431] [Citation(s) in RCA: 5] [Impact Index Per Article: 1.3] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Received: 01/11/2021] [Revised: 03/19/2021] [Accepted: 04/12/2021] [Indexed: 12/22/2022]

Sherf-Dagan S, Sinai T, Goldenshluger A, Globus I, Kessler Y, Schweiger C, Ben-Porat T. Nutritional Assessment and Preparation for Adult Bariatric Surgery Candidates: Clinical Practice. Adv Nutr 2021;12:1020-1031. [PMID: 33040143 PMCID: PMC8262552 DOI: 10.1093/advances/nmaa121] [Citation(s) in RCA: 12] [Impact Index Per Article: 3.0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 06/01/2020] [Revised: 07/25/2020] [Accepted: 09/03/2020] [Indexed: 12/13/2022] Open

Abstract

Bariatric surgery (BS) has proven to be highly efficacious in the treatment of obesity and its comorbidities. However, careful patient selection is critical for its success. Thus, patients should undergo medical, behavioral, and nutritional assessment by a multidisciplinary team. From the nutritional point of view, BS candidates should undergo nutritional assessment, preparation, and education by a registered dietitian in the preoperative period. Currently, detailed specified and comprehensive information on these topics is lacking. The present narrative review aimed to summarize the available literature concerning both the preoperative nutritional assessment components and the preoperative nutritional preparation and education components of patients planning to undergo BS. Current literature indicates that proper management before BS should include a comprehensive nutritional assessment, in which it is advisable to perform a clinical interview to assess patients' medical background, weight management history, eating patterns and pathologies, oral health, physical activity habits, nutritional status, supplementation usage, BS knowledge, surgery expectations and anthropometric measurements. Nutritional preparation and educational strategies should include an individualized preoperative weight-loss nutrition program, improvement of glycemic control, micronutrients deficiencies correction, eating and lifestyle habits adaptation, physical activity initiation, and strengthening knowledge on obesity and BS. At this stage, more well-designed intervention and long-term cohort studies are needed in order to formulate uniform evidence-based nutritional guidelines for patients who plan to undergo BS, including populations at higher nutritional risk. Moreover, postoperative outcomes of presurgical nutritional intervention programs should be studied.

Collapse

Wongdee K, Chanpaisaeng K, Teerapornpuntakit J, Charoenphandhu N. Intestinal Calcium Absorption. Compr Physiol 2021;11:2047-2073. [PMID: 34058017 DOI: 10.1002/cphy.c200014] [Citation(s) in RCA: 26] [Impact Index Per Article: 6.5] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 01/01/2023]

Ai T, Hao L, Shang L, Wang L, Li B, Li J. Konjac Oligosaccharides Modulate the Gut Environment and Promote Bone Health in Calcium-Deficient Mice. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 2021;69:4412-4422. [PMID: 33832226 DOI: 10.1021/acs.jafc.0c07839] [Citation(s) in RCA: 17] [Impact Index Per Article: 4.3] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 06/12/2023]

Boby N, Abbas MA, Lee EB, Im ZE, Hsu WH, Park SC. Protective Effect of Pyrus ussuriensis Maxim. Extract against Ethanol-Induced Gastritis in Rats. Antioxidants (Basel) 2021;10:antiox10030439. [PMID: 33809380 PMCID: PMC8002011 DOI: 10.3390/antiox10030439] [Citation(s) in RCA: 14] [Impact Index Per Article: 3.5] [Reference Citation Analysis] [Abstract] [Key Words] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 01/29/2021] [Revised: 03/05/2021] [Accepted: 03/09/2021] [Indexed: 12/16/2022] Open

Abstract

Pyrus ussuriensis Maxim (Korean pear) has been used for hundreds of years as a traditional herbal medicine for asthma, cough, and atopic dermatitis in Korea and China. Although it was originally shown to possess anti-inflammatory, antioxidant, and antiatopic properties, its gastroprotective effects have not been investigated. In the present study, we evaluated the protective effects of Pyrus ussuriensis Maxim extract (PUE) against ethanol-induced gastritis in rats. The bioactive compound profile of PUE was determined by gas chromatography mass spectroscopy (GC-MS) and high-performance liquid chromatography (HPLC). The gastroprotection of PUE at different doses (250 and 500 mg/kg body weight) prior to ethanol ingestion was evaluated using an in vivo gastritis rat model. Several endpoints were evaluated, including gastric mucosal lesions, cellular degeneration, intracellular damage, and immunohistochemical localization of leucocyte common antigen. The gastric mucosal injury and ulcer score were determined by evaluating the inflamed gastric mucosa and by histological examination. To identify the mechanisms of gastroprotection by PUE, antisecretory action and plasma prostaglandin E₂ (PGE₂), gastric mucosal cyclic adenosine monophosphate (cAMP), and histamine levels were measured. PUE exhibited significant antioxidant effects with IC₅₀ values of 56.18 and 22.49 µg/mL for 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′- azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid (ABTS) inhibition (%), respectively. In addition, GC/MS and HPLC analyses revealed several bioactive compounds of PUE. Pretreatment with PUE significantly (p < 0.05) decreased the ulcer index by preventing gastric mucosal lesions, erosion, and cellular degeneration. An immunohistochemical analysis revealed that PUE markedly attenuated leucocyte infiltration in a dose-dependent manner. The enhancement of PGE₂ levels and attenuation of cAMP levels along with the inhibition of histamine release following PUE pretreatment was associated with the cytoprotective and healing effects of PUE. In contrast, the downregulation of the H⁺/K⁺ ATPase pathway as well as muscarinic receptor (M₃R) and histamine receptor (H₂R) inhibition was also involved in the gastroprotective effects of PUE; however, the expression of cholecystokinin-2 receptors (CCK₂R) was unchanged. Finally, no signs of toxicity were observed following PUE treatment. Based on our results, we conclude that PUE represents an effective therapeutic option to reduce the risk of gastritis and warrants further study.

Collapse