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Jarmakiewicz-Czaja S, Zielińska M, Helma K, Sokal A, Filip R. Effect of Nigella sativa on Selected Gastrointestinal Diseases. Curr Issues Mol Biol 2023; 45:3016-3034. [PMID: 37185722 PMCID: PMC10136991 DOI: 10.3390/cimb45040198] [Citation(s) in RCA: 3] [Impact Index Per Article: 1.5] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 02/23/2023] [Revised: 03/28/2023] [Accepted: 03/30/2023] [Indexed: 04/07/2023] Open
Abstract
Nigella sativa L. (family Ranunculaceae), also known as black cumin, has been used in cuisine around the world for many years. Due to its health-promoting properties, it can be used not only in the food industry but also in medicine. The main bioactive compound contained in the black cumin extract is thymoquinone (TQ), which has a special therapeutic role. The results of research in recent years confirmed its hypoglycemic, hypolipemic, and hepatoprotective effects, among others. In addition, the results of laboratory tests also indicate its immunomodulatory and anticancer effects, although there is still a lack of data on the mechanisms of how they are involved in the fight against cancer. Including this plant material in one’s diet can be both an element of prophylaxis and therapy supporting the treatment process, including pharmacological treatment. However, attention should be paid to its potential interactions with drugs used in the treatment of chronic diseases.
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Affiliation(s)
| | - Magdalena Zielińska
- Institute of Health Sciences, Medical College of Rzeszow University, 35-959 Rzeszow, Poland
| | - Kacper Helma
- Institute of Health Sciences, Medical College of Rzeszow University, 35-959 Rzeszow, Poland
| | - Aneta Sokal
- Institute of Health Sciences, Medical College of Rzeszow University, 35-959 Rzeszow, Poland
| | - Rafał Filip
- Institute of Medicine, Medical College of Rzeszow University, 35-959 Rzeszow, Poland
- Department of Gastroenterology with IBD Unit, Clinical Hospital No. 2, 35-301 Rzeszow, Poland
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Antioxidant Activity of Urtica dioica: An Important Property Contributing to Multiple Biological Activities. Antioxidants (Basel) 2022; 11:antiox11122494. [PMID: 36552702 PMCID: PMC9774934 DOI: 10.3390/antiox11122494] [Citation(s) in RCA: 10] [Impact Index Per Article: 3.3] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 11/23/2022] [Revised: 12/14/2022] [Accepted: 12/16/2022] [Indexed: 12/23/2022] Open
Abstract
Urtica dioica (UD) is a multi-functional plant known to be used as both food and medicine from ancient times. The plant has the potential to be used as a fertilizer and for biological pest control. It is also used in textile and related industries for its quality fibers. In the recent past, the plant has received great attention for its numerous important biological activities and food applications. The antioxidant activity of UD is the crucial factor supporting its important biological activities, such as anticancer, antidiabetic and anti-inflammatory properties. The antioxidant activity of UD is also found to be protective in different organs, including the brain, liver, lungs, kidney, ovary, and uterus, and may also be protective against diseases associated with these organs. Few clinical studies have endorsed the antioxidant potential of UD in patients. The current work is an attempt to comprehensively compile and discuss the antioxidant activity of UD from in vitro, in vivo and human studies. The insights of the current study would be helpful in getting a panoramic view of the antioxidant potential of UD, and provide direction for optimizing and developing it for therapeutic applications against important diseases and conditions in the near future.
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Wang X, Peng SM, Liu Y, Liao S, Zhao HH, Duan GY, Wu YM, Liu CJ, Wang YZ, Liu TM, Li YH, Fan ZY, Zhu SY, Qiu HJ, Lin Q. Effect of Ramie on the Production Performance of Laying Hens, and the Quality, Nutrient Composition, Antioxidation of the Eggs. Front Physiol 2022; 13:854760. [PMID: 35707011 PMCID: PMC9189287 DOI: 10.3389/fphys.2022.854760] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Grants] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 01/14/2022] [Accepted: 04/21/2022] [Indexed: 11/13/2022] Open
Abstract
Ramie (Boehmeria nivea), which is rich in protein, fatty acid, vitamins and minerals, has become a potential alternative feed resource for poultry, and has attracted more and more attentions in nutrition research. The objective of this study is to evaluate the effect of dietary ramie at different concentrations on the production performance of the hens, and the quality, nutrient composition, and antioxidation of the eggs. A total of 432 34-week-old Lohmann commercial laying hens were divided into four groups, that were fed with corn-soybean meal-based control diet, control mixed with ramie at concentrations of 3, 6, or 9% separately for 8 weeks. Results showed that dietary ramie did not affect production performance. And egg yolk color gradually deepened as the inclusion levels of ramie increased. Ramie at tested concentration could significantly reduce the content of malondialdehyde (MDA) (p = 0.002) and 3% ramie supplementation significantly increased total antioxidative capacity (T-AOC) concentrations in egg yolk compared to the control group (p = 0.033). In addition, dietary supplementation with 6% ramie significantly reduced total cholesterol (T-CHO) content (p < 0.05) compared with controls. For egg nutrient composition, compared with the control group, the addition of 6% ramie significantly increased (p < 0.05) total omega-3 polyunsaturated fatty acids (n-3PUFA) and phenylalanine (Phe) in yolk. In conclusion, dietary inclusion of 6% ramie was most effective in improving the color, antioxidative capability, and reducing T-CHO contents of the egg yolks without any negative impacts on the production performance of the hens.
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Affiliation(s)
- Xin Wang
- Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha, China
| | - Si-Min Peng
- Hunan Deren Husbandry Technology Co., Ltd., Changde, China
| | - Yang Liu
- College of Animal Science and Technology, Hunan Agriculture University, Changsha, China
| | - Shuang Liao
- Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha, China
| | - Hao-Han Zhao
- Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha, China
| | - Guang-Ying Duan
- Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha, China
- College of Animal Science and Technology, Hunan Agriculture University, Changsha, China
| | - Yong-Mei Wu
- Hunan Deren Husbandry Technology Co., Ltd., Changde, China
| | - Chun-Jie Liu
- Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha, China
| | - Yan-Zhou Wang
- Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha, China
| | - Tou-Ming Liu
- Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha, China
| | - Ying-Hui Li
- College of Animal Science and Technology, Hunan Agriculture University, Changsha, China
| | - Zhi-Yong Fan
- College of Animal Science and Technology, Hunan Agriculture University, Changsha, China
| | - Si-Yuan Zhu
- Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha, China
- *Correspondence: Si-Yuan Zhu, ; Hua-Jiao Qiu, ; Qian Lin,
| | - Hua-Jiao Qiu
- Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha, China
- *Correspondence: Si-Yuan Zhu, ; Hua-Jiao Qiu, ; Qian Lin,
| | - Qian Lin
- Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha, China
- Hunan Deren Husbandry Technology Co., Ltd., Changde, China
- *Correspondence: Si-Yuan Zhu, ; Hua-Jiao Qiu, ; Qian Lin,
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The effect of Nigella sativa on TAC and MDA in obese and overweight women: secondary analysis of a crossover, double blind, randomized clinical trial. J Diabetes Metab Disord 2022; 21:171-179. [PMID: 35673509 PMCID: PMC9167332 DOI: 10.1007/s40200-021-00954-5] [Citation(s) in RCA: 8] [Impact Index Per Article: 2.7] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Received: 08/26/2021] [Accepted: 12/02/2021] [Indexed: 10/19/2022]
Abstract
Purpose Since obesity is a risk factor for various diseases and is associated with increased oxidative stress conditions, some herbs are considered to be effective in reducing obesity and its complications. Methods This secondary analysis investigates the effect of Nigella sativa (N.S) oil supplement on total antioxidant capacity (TAC) and malondialdehyde (MDA) levels in obese/overweight women. Obese and overweight healthy women were randomized to receive 2,000 mg/d of N.S supplement and placebo. The intervention periods lasted 8 weeks and were separated by a 4-week washout period. Also, each participant was given an iso-calorie diet. Baseline characteristics and TAC and MDA levels were measured. Pkcross analysis was performed for statistical analysis using Stata software. Also, Cohen's d was estimated as effect size for all results to assess the magnitude of the effects. Results 39 women completed the study. N.S oil supplementation at a dose of 2000 mg/d significantly increased serum TAC (P effect = 0.017, Cohen's d = 1.81) and reduced serum MDA (P effect < 0/001, Cohen's d = - 0.32). Conclusion Based on our findings taking N.S supplementation for 8 weeks can improve antioxidant conditions in obese and overweight adults. However, more studies with a larger population and the presence of both genders need to be done to confirm the results.Registration number: IRCT20180430039475N1.
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Mustari A, Nooruzzaman M, Miah MA, Sujan KM, Chowdhury EH. Promoting action of vitamin E and black seed oil on reproductive hormones and organ histoarchitecture of Swiss albino mice. Vet Med Sci 2022; 8:710-718. [PMID: 35037408 PMCID: PMC8959291 DOI: 10.1002/vms3.708] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.3] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 12/25/2022] Open
Abstract
Background Vitamin E and black seed oil are two powerful antioxidants with several health benefits. Objective The effect of vitamin E and black seed oil on reproductive performance of Swiss albino mice was studied. Methods A total of 80 (40 male and 40 female) mice of 25–28 days old were randomly divided into four groups viz., A, B, C and D consisting of 10 mice in each group. Mice from the group A served as vehicle control and received normal mice ration whereas mice from the group B, C and D received feed supplemented with either black seed oil (0.5 ml/kg), vitamin E (200 mg/kg) or combination of black seed oil (0.5 ml/kg) and vitamin E (200 mg/kg), respectively daily for 16 weeks. At the end point of the study, blood samples were collected and sera were separated for hormonal analysis. At the same time, mice were sacrificed and testes and ovaries were collected for histomorphological examination. Results In male mice, the level of testosterone increased significantly in mice receiving black seed oil only, whereas the thyroxin increased significantly in all treated groups when compared to the control mice. Histomorphological examination revealed a significant increase in the diameter of seminiferous tubules in male mice fed with either black seed oil or vitamin E or both. On the other hand, the oestradiol and thyroxin concentration in female mice showed no significant changes in both control and treated groups. However, ovaries of mice fed with black seed oil or vitamin E or both showed an increased number of the follicles of different stages than the control mice. Conclusions The findings highlighted the promoting action of vitamin E and black seed oil on reproductive functions of mice and that can be used to treat infertility in man and animals.
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Affiliation(s)
- Afrina Mustari
- Department of Physiology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh, Bangladesh
| | - Mohammed Nooruzzaman
- Department of Pathology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh, Bangladesh
| | - Mohammad Alam Miah
- Department of Physiology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh, Bangladesh
| | - Khaled Mahmud Sujan
- Department of Physiology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh, Bangladesh
| | - Emdadul Hauqe Chowdhury
- Department of Pathology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh, Bangladesh
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Antibacterial Activity and Time-kill Assay of Terminalia catappa L. and Nigella sativa L. against Selected Human Pathogenic Bacteria. JOURNAL OF PURE AND APPLIED MICROBIOLOGY 2021. [DOI: 10.22207/jpam.15.1.22] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.3] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 01/02/2023] Open
Abstract
The current investigation aims to test the susceptibility of human pathogenic clinical isolates and MTCC strains to leaf and seed extracts of Terminalia catappa and Nigella sativa. Disc diffusion assay, micro dilution assay and minimum Bactericidal Concentration investigated the susceptibility of bacteria to the test extracts. The active extract was subjected to phytochemical screening, separation of the phytochemicals by Thin Layer Chromatography, bioactivity guided assay and Time- kill assay. Acetone and methanol extracts of T.catappa revealed, significant inhibition of clinical origin Staphylococcus aureus followed by Proteus vulgaris and the MTCC strains Staphylococcus aureus, Salmonella typhi, Pseudomonas aeroginosa and Bacillus subtilis. Nigella sativa inhibited the growth of clinical origin Staph.aureus and MTCC strain of Staph.aureus, Salmonella typhi and B.subtilis. Minimum inhibitory concentration for all the test bacteria was reported in the range of 5000μg/ml to 9 μg/ml in T. catappa extract. Most sensitive being the clinical isolate Staph. aureus and Proteus vulgaris. The bactericidal concentration for the test bacteria was found to be between 5000μg/ml and 625μg/ml. Phyto-chemical analysis of leaf extracts of T. catappa found to have dominated by polyphenols (Terpenoids, steroids, flavonoids, flavones, saponins and tannins) and N.sativa extracts recorded the presence of alkaloids, proteins and oils and fats. TLC profiling of the acetone extract revealed many antibacterial active bands. Bands having Retention factor 0.47 and 0.52 were active against the test bacteria. Time kill assay of the acetone extract of T. catappa were carried out for the first time. The extract exhibited dose dependent bactericidal and bacteriostatic activity against the clinical isolates.
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Alagawany M, Elnesr SS, Farag MR, Abd El-Hack ME, Khafaga AF, Sharun K, Marappan G, Dhama K. Health-Promoting Activities of Nigella sativa Essential Oil. BLACK CUMIN (NIGELLA SATIVA) SEEDS: CHEMISTRY, TECHNOLOGY, FUNCTIONALITY, AND APPLICATIONS 2021:457-478. [DOI: 10.1007/978-3-030-48798-0_29] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 09/02/2023]
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Dorsaf H, Sabrine M, Houda BL, Khémais BR, Mohsen S, Olfa T. Pecan pericarp extract protects against carbon tetrachloride-induced liver injury through oxidative mechanism in rats. Toxicol Res (Camb) 2020; 9:652-660. [PMID: 33178425 PMCID: PMC7640928 DOI: 10.1093/toxres/tfaa071] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.2] [Reference Citation Analysis] [Abstract] [Key Words] [Grants] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 05/05/2020] [Revised: 07/22/2020] [Accepted: 08/15/2020] [Indexed: 11/12/2022] Open
Abstract
The purpose of this study was to quantify the proanthocyanidin content of pecan (Carya illinoinensis) pericarp extract (PPE) and to assess its useful impacts against carbon tetrachloride (CCl4)-induced hepatotoxicity. Rats were randomly divided into four groups: Group 1: received intraperitoneal injection of saline solution, Group 2: was injected with PPE (25 mg/kg body weight) for 10 consecutive days, Group 3: received CCl4 (0.5 ml/kg, subcutaneous injection), Group 4: was coadministred with PPE + CCl4. The CCl4 was administered every 3 days during 10 days. Results revealed the presence of a high amount of total proanthocyanidins in the PPE (81.01 ± 0.21 mg TAE.g-1DW). CCl4 injection induced significant reductions in hepatic antioxidants but increased hepatic lipid peroxidation (LPO) as well as serum injury biomarkers. However, cotreatment with PPE significantly (P < 0.05) inverted CCl4-induced increase in plasma alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, and lactate dehydrogenase activities, respectively to 74%, 77%, 60%, and 82% compared with CCl4 group. No significant toxic effects were observed following treatment with plant extract alone. PPE cotreatment also decreased significant (P < 0.05) the hepatic malondialdehyde formation (21%) and enhanced the liver catalase activity (107%) in CCl4-intoxicated rats. The histopathological examination showed inflammatory infiltration and degenerative changes in the hepatic tissue following CCl4 injection. The hepatoprotective activity of PPE against CCl4 exposure was supported by the maintenance of structural integrity of liver histopathology. In conclusion, the current study illustrated that PPE pretreatment significantly improved all examined parameters, restored the hepatic architecture and successfully alleviates oxidative damage induced by CCl4 intoxication.
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Affiliation(s)
- Hallegue Dorsaf
- Laboratory of Integrated Physiology, Faculty of Sciences of Bizerte, University of Carthage, Jarzouna, Bizerte 7021, Tunisia
| | - Moujahed Sabrine
- Laboratory of Integrated Physiology, Faculty of Sciences of Bizerte, University of Carthage, Jarzouna, Bizerte 7021, Tunisia
| | - Ben Lamine Houda
- Laboratory of Pathologic Anatomy, Menzel Bourguiba Hospital, 5 Palestina Road, Menzel Bourguiba, Bizerte 7050, Tunisia
| | - Ben Rhouma Khémais
- Laboratory of Integrated Physiology, Faculty of Sciences of Bizerte, University of Carthage, Jarzouna, Bizerte 7021, Tunisia
| | - Sakly Mohsen
- Laboratory of Integrated Physiology, Faculty of Sciences of Bizerte, University of Carthage, Jarzouna, Bizerte 7021, Tunisia
| | - Tebourbi Olfa
- Laboratory of Integrated Physiology, Faculty of Sciences of Bizerte, University of Carthage, Jarzouna, Bizerte 7021, Tunisia
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Kilany OE, El-Beltagy MA, El-Sherbeeny NA. Tribulus terrestris ameliorates carbon tetrachloride-induced hepatotoxicity in male rats through suppression of oxidative stress and inflammation. ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH INTERNATIONAL 2020; 27:24967-24981. [PMID: 32342415 DOI: 10.1007/s11356-020-08826-w] [Citation(s) in RCA: 11] [Impact Index Per Article: 2.2] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 12/22/2019] [Accepted: 04/07/2020] [Indexed: 06/11/2023]
Abstract
Hepatoprotection is a goal for the harmful effect of several hepatotoxic agents. The present study has been executed to assess the useful impacts of Tribulus terrestris (TT) and silymarin (SLM) against carbon tetrachloride (CCL4)-induced hepatotoxicity. Forty-two male rats were partitioned into six groups: group I: received 0.3% CMC-Na in distilled water, group II: TT (500 mg/kg BW, orally), group III: SLM (200 mg/kg, orally) for 14 consecutive days (on days 11 and 12 intraperitoneal corn oil), group IV: CCL4, group V: TT (500 mg/kg BW) plus CCL4, and group VI: SLM (200 mg/kg orally) plus CCL4. The CCL4 was administered (2.0 ml/kg BW) intraperitoneal on days 11 and 12. Sera were collected for assessment of hepatic injury markers and pro-inflammatory cytokines. Additionally, liver tissue oxidative stress, lipid peroxidation, histopathological examination, and immunohistochemical analysis (Bax and bcl-2) were done. CCL4 injection induced significant reductions in hepatic antioxidants while increased hepatic lipid peroxidation as well as serum hepatic injury biomarkers and pro-inflammatory cytokines. The histopathological examination showed necrotic and degenerative changes in the hepatic tissue, while immunohistochemical analysis revealed marked hepatic expression of activated Bax, and bcl-2, following CCL4 injection. TT pretreatment significantly improved all examined parameters and restored the hepatic architecture. The current study illustrated that TT effectively alleviates hepatic oxidative damage, apoptosis, and inflammation, induced by acute CCL4 intoxication. In this manner, TT has promising cytoprotective powers against hepatotoxicity induced by CCL4.
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Affiliation(s)
- Omnia E Kilany
- Department of Clinical Pathology, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, 41522, Egypt.
| | - Marwa A El-Beltagy
- Department of Biochemistry, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, 41522, Egypt
| | - Nagla A El-Sherbeeny
- Department of Clinical Pharmacology, Faculty of Medicine, Suez Canal University, Ismailia, 41522, Egypt
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Dhouibi R, Affes H, Ben Salem M, Hammami S, Sahnoun Z, Zeghal KM, Ksouda K. Screening of pharmacological uses of Urtica dioica and others benefits. PROGRESS IN BIOPHYSICS AND MOLECULAR BIOLOGY 2020; 150:67-77. [DOI: 10.1016/j.pbiomolbio.2019.05.008] [Citation(s) in RCA: 21] [Impact Index Per Article: 4.2] [Reference Citation Analysis] [Track Full Text] [Subscribe] [Scholar Register] [Received: 05/03/2019] [Revised: 05/22/2019] [Accepted: 05/29/2019] [Indexed: 01/13/2023]
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Mangwani N, Singh PK, Kumar V. Medicinal plants: Adjunct treatment to tuberculosis chemotherapy to prevent hepatic damage. J Ayurveda Integr Med 2019; 11:522-528. [PMID: 31679802 PMCID: PMC7772497 DOI: 10.1016/j.jaim.2019.02.004] [Citation(s) in RCA: 18] [Impact Index Per Article: 3.0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 08/07/2018] [Revised: 12/29/2018] [Accepted: 02/18/2019] [Indexed: 02/07/2023] Open
Abstract
The effectiveness of herbs for the management of chemically induced hepatotoxicity has been discussed by many researchers. However, there is a paucity of compressive literature on the significance of hepatoprotective plants for the management of anti-TB drug induced toxicity. Anti-TB drugs have been reported to causes hepatic damage, due to which, many patients across the globe discontinued the treatment. Medicinal plants have multiple therapeutic effects. The assessment of biological activity of plants against Mycobacterium and its use for hepatic recovery provides an effective treatment approach. Traditionally used medicinal plants are the rich source of phytochemicals and secondary metabolites. These compounds can restore normal function, enzymatic activity and structure of hepatic cells against anti-TB drug induced hepatotoxicity. The present review covers comprehensive details on different hepatoprotective and antimycobacterial plants studied during past few decades so that potential adjuvants can be studied for Tuberculosis chemotherapy.
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Affiliation(s)
- Neelam Mangwani
- Value Addition Research and Development-Human Health, National Innovation Foundation-India, Grambharti, Mahudi Road, Gandhinagar, 382650, Gujarat, India
| | - Pawan Kumar Singh
- Value Addition Research and Development-Human Health, National Innovation Foundation-India, Grambharti, Mahudi Road, Gandhinagar, 382650, Gujarat, India.
| | - Vipin Kumar
- Value Addition Research and Development-Human Health, National Innovation Foundation-India, Grambharti, Mahudi Road, Gandhinagar, 382650, Gujarat, India
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Cherif M, Valenti B, Abidi S, Luciano G, Mattioli S, Pauselli M, Bouzarraa I, Priolo A, Ben Salem H. Supplementation of Nigella sativa seeds to Barbarine lambs raised on low- or high-concentrate diets: Effects on meat fatty acid composition and oxidative stability. Meat Sci 2018; 139:134-141. [DOI: 10.1016/j.meatsci.2018.01.022] [Citation(s) in RCA: 16] [Impact Index Per Article: 2.3] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 10/12/2017] [Revised: 01/23/2018] [Accepted: 01/24/2018] [Indexed: 10/18/2022]
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Srinivasan K. Cumin (Cuminum cyminum) and black cumin (Nigella sativa) seeds: traditional uses, chemical constituents, and nutraceutical effects. FOOD QUALITY AND SAFETY 2018; 2:1-16. [DOI: 10.1093/fqsafe/fyx031] [Citation(s) in RCA: 73] [Impact Index Per Article: 10.4] [Reference Citation Analysis] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 01/04/2025]
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Inhibition of cytochrome P450 enzymes by thymoquinone in human liver microsomes. Saudi Pharm J 2018; 26:673-677. [PMID: 29989011 PMCID: PMC6035319 DOI: 10.1016/j.jsps.2018.02.024] [Citation(s) in RCA: 18] [Impact Index Per Article: 2.6] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 12/28/2017] [Accepted: 02/11/2018] [Indexed: 12/18/2022] Open
Abstract
The aim of the present study was to investigate the potential effect of thymoquinone (TQ) on the metabolic activity of four major drug metabolizing enzymes in human liver microsomes, namely cytochrome P450 (CYP) 1A2, CYP2C9, CYP2D6 and CYP3A4. The inhibition of CYP enzymatic activities by TQ was evaluated by incubating typical substrates (phenacetin for CYP1A2, tolbutamide for CYP2C9, dextromethorphan for CYP2D6, and testosterone for CYP3A4) with human liver microsomes and NADPH in the absence or presence of TQ (1, 10 and 100 µM). The respective metabolite of the substrate that was formed was measured by HPLC. Results of the presented study presented that the metabolic activities of all the investigated CYP enzymes, viz. CYP1A2, CYP2C9, CYP2D6 and CYP3A4, were inhibited by TQ. At 1 µM TQ, CYP2C9 enzyme activity was maximally inhibited by 46.35%, followed by CYP2D6 (20.26%) > CYP1A2 (13.52%) > CYP3A4 (12.82%). However, at 10 µM TQ, CYP2C9 enzyme activity was maximally inhibited by 69.69%, followed by CYP3A4 (23.59%) > CYP1A2 (23.51%) > CYP2D6 (11.42%). At 100 µM TQ, CYP1A2 enzyme activity was maximally inhibited by 81.92%, followed by CYP3A4 (79.24%) > CYP2C9 (69.22%) > CYP2D6 (28.18%). The IC50 (mean ± SE) values for CYP1A2, CYP2C9, CYP2D6 and CYP3A4 inhibition were 26.5 ± 2.9 µM, 0.5 ± 0.4 µM, >500 µM and 25.2 ± 3.1 µM, respectively. These findings suggest that there is a high probability of drug interactions resulting from the co-administration of TQ or herbs containing TQ with drugs that are metabolized by the CYP enzymes, particularly CYP2C9.
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Usta A, Dede S. The Effect of Thymoquinone on Nuclear Factor Kappa B Levels and Oxidative DNA Damage on Experimental Diabetic Rats. Pharmacogn Mag 2017; 13:S458-S461. [PMID: 29142399 PMCID: PMC5669082 DOI: 10.4103/pm.pm_134_17] [Citation(s) in RCA: 8] [Impact Index Per Article: 1.0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 04/10/2017] [Revised: 05/17/2017] [Indexed: 12/19/2022] Open
Abstract
Background: Thymoquinone (TQ), the basic bioactive phytochemical constituent of seed oil of Nigella sativa, is one of these herbal drugs known for antidiabetic effects. This study was carried out to assess the effects of the possible role of TQ on nuclear factor kappa B (NF-κB) and oxidative DNA damage levels in experimental diabetic rats. Materials and Methods: Twenty-eight male Wistar Albino rats (200–250 g) were used as experimental subjects. The rats were divided into four groups, including the control, control supplemented with TQ (CT), diabetic (D), and diabetic supplemented with TQ (DT), each containing seven rats. The D and the DT groups were treated with 45 mg/kg streptozotocin (STZ) (intraperitoneal). TQ was administered 30 mg/kg/day for 21 days by oral gavage in the DT and the T groups. Results: It was determined that glucose, glycosylated hemoglobin (HbA1c) levels and alanine aminotransferase, aspartate aminotransferase, and gamma-glutamyl transpeptidase activities were decreased significantly and approached the control group in the DT group after TQ supplement (P < 0.05). Urea levels were the lowest in CT (P < 0.05). Oxidative DNA damage (8 hydroxy-2-deoxyguanosine) was increased in both of the diabetic groups (D and DT). The NF-κB levels were the highest in Group D (P < 0.05). Conclusion: It was observed that increased glucose and HbA1c levels and the indicators of liver and kidney damages were decreased significantly after TQ supplementation. Oxidative DNA damage and NF-κB levels were increased in the diabetic group, and TQ administration caused a statistically insignificant reduction. SUMMARY
In this study, the effects of thymoquinone (TQ), the basic bioactive phytochemical constituent of seed oil of Nigella sativa, on nuclear factor kappa B (NF-κB), oxidative DNA damage levels, and, some biochemical parameters was invesigated. It was observed that some biochemical parameters (glucose, glycosylated hemoglobin (HbA1c), ALT, AST, GGT) were close to the control group after TQ treatment in diabetic group. Oxidative DNA damage (8 hydroxy 2 deoxyguanosine) and NF-κB were highest levels and TQ implementation caused statistically insignificant decrease, in the diabetic group.
Abbreviations used: 8-OHdG: 8 hydroxi-2-deoxiguanosin; ALT: Alanine aminotransferase; AST: Aspartate aminotransferase; GGT: Gamma-glutamyl transpeptidase; HbA1c: Glycosylated hemoglobin; NF-κB: Nuclear factor kappa protein; STZ: Streptozotocin; TQ: Thymoquinone.
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Affiliation(s)
- Ayşe Usta
- Department of Chemistry, Faculty of Science, Yuzuncu Yil University, Van, Turkey
| | - Semiha Dede
- Department of Biochemistry, Yuzuncu Yil University, Faculty of Veterinary Medicine, Van, Turkey
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A G, S D. THE SERUM PROTEIN FRACTIONS IN THYMOQUINONE TREATED RATS. AFRICAN JOURNAL OF TRADITIONAL, COMPLEMENTARY, AND ALTERNATIVE MEDICINES 2017; 13:27-31. [PMID: 28480357 PMCID: PMC5412198 DOI: 10.21010/ajtcam.v13i6.6] [Citation(s) in RCA: 3] [Impact Index Per Article: 0.4] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Subscribe] [Scholar Register] [Indexed: 11/29/2022]
Abstract
Background: TQ has been used as treatment and preventive agent for many diseases over the years. The goal of this study was to investigate the effects of TQ supplement on fractions of serum proteins. Materials and methods: Fourteen male Wistar-Albino rats (200-250 g weight) were used as material for two groups; (control (C) and thymoquinone (TQ) respectively. Each group contained seven rats. The control group had only corn oil, while the TQ group was dissolved in corn oil. 30 mg/kg/day were given by oral gavage for four weeks. The serum protein fractions were identified using cellulose acetate technique. Results: The total protein level and albumin, α-1, α-2 fractions and A/G ratio have showed no difference between groups (p>0.05). β-globulin fractions of TQ group were higher than control’s (p<0.05). In addition, it was observed that the γ-globulin levels of TQ group were lower than that of the control group’s (p<0.05). Conclusion: From the results, it was observed that the changes of these fractions may have originated from elevation or decline synthesis, or activities of containing proteins.
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Affiliation(s)
- Güllü A
- Biochemistry Department, Veterinary Medical Faculty, Yuzuncu Yil University, Van, 65080, Turkey
| | - Dede S
- Biochemistry Department, Veterinary Medical Faculty, Yuzuncu Yil University, Van, 65080, Turkey
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Saxena S, Rathore S, Diwakar Y, Kakani R, Kant K, Dubey P, Solanki R, Sharma L, Agarwal D, John S. Genetic diversity in fatty acid composition and antioxidant capacity of Nigella sativa L. genotypes. Lebensm Wiss Technol 2017. [DOI: 10.1016/j.lwt.2016.12.033] [Citation(s) in RCA: 12] [Impact Index Per Article: 1.5] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/24/2022]
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Abdelrazek HM, Helmy SA, Elsayed DH, Ebaid HM, Mohamed RM. Ameliorating effects of green tea extract on cadmium induced reproductive injury in male Wistar rats with respect to androgen receptors and caspase- 3. Reprod Biol 2016; 16:300-308. [DOI: 10.1016/j.repbio.2016.11.001] [Citation(s) in RCA: 41] [Impact Index Per Article: 4.6] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 04/11/2016] [Revised: 10/30/2016] [Accepted: 11/02/2016] [Indexed: 12/24/2022]
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Akram Khan M, Afzal M. Chemical composition of Nigella sativa Linn: Part 2 Recent advances. Inflammopharmacology 2016; 24:67-79. [PMID: 27068721 PMCID: PMC4883276 DOI: 10.1007/s10787-016-0262-7] [Citation(s) in RCA: 49] [Impact Index Per Article: 5.4] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 02/02/2016] [Accepted: 03/15/2016] [Indexed: 01/15/2023]
Abstract
The black cumin or Nigella sativa L. seeds have many acclaimed medicinal properties such as bronchodilatory, hypotensive, antibacterial, antifungal, analgesic, anti-inflammatory and immunopotentiating. This review article is an update on the previous article published on Nigella sativa L. in this journal in 1999. It covers the medicinal properties and chemical syntheses of the alkaloids isolated from the seeds of the herb.
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Affiliation(s)
- M Akram Khan
- Biomolecular Science Centre, Sheffield Hallam University, Howard Street, Sheffield, S1 1WB, UK.
| | - M Afzal
- Department of Biological Sciences, Faculty of Science, Kuwait University, Kuwait City, Kuwait
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Mahdavi R, Namazi N, Alizadeh M, Farajnia S. Nigella sativa oil with a calorie-restricted diet can improve biomarkers of systemic inflammation in obese women: A randomized double-blind, placebo-controlled clinical trial. J Clin Lipidol 2015; 10:1203-11. [PMID: 27678438 DOI: 10.1016/j.jacl.2015.11.019] [Citation(s) in RCA: 42] [Impact Index Per Article: 4.2] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 07/16/2015] [Revised: 11/26/2015] [Accepted: 11/28/2015] [Indexed: 12/30/2022]
Abstract
BACKGROUND Inflammation is one of the primary mechanisms in the development of metabolic complications. Although anti-inflammatory characteristics of Nigella sativa (NS) have been indicated in animal models, clinical trials related to the effects of NS on inflammatory parameters are relatively scarce. OBJECTIVE The aim of the present study was to determine the effects of NS oil combined with a calorie-restricted diet on systemic inflammatory biomarkers in obese women. METHODS In this double-blind placebo-controlled randomized clinical trial, 90 volunteer obese (body mass index = 30-34.9 kg/m(2)) women aged 25-50 years were recruited. Participants were randomly divided into two groups, an intervention group (n = 45) and a placebo group (n = 45). Each group received either: (1) a low-calorie diet with 3 g/day of NS oil or (2) a low-calorie diet with 3 g/day placebo for 8 weeks. RESULTS A total of 84 females (intervention group = 43; placebo group = 41) completed the trial. Subjects in the intervention group did not report any side effects with the NS oil supplementation. NS oil decreased serum levels of tumor necrosis factor-alpha (-40.8% vs -16.1%, P = .04) and high-sensitivity C-reactive protein (-54.5% vs -21.4%, P = .01) compared to the placebo group. However, there were no significant changes in interleukin-6 levels (-8.6 vs -2.4%, P = .6) in the NS group compared to the placebo group. CONCLUSIONS NS oil supplementation combined with a calorie-restricted diet may modulate systemic inflammatory biomarkers in obese women. However, more studies are needed to clarify the efficacy of NS oil as an adjunct therapy to improve inflammatory parameters in obese subjects.
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Affiliation(s)
- Reza Mahdavi
- Nutrition Research Center, Faculty of Nutrition, Tabriz University of Medical Sciences, Tabriz, Iran
| | - Nazli Namazi
- Nutrition Research Center, Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran.
| | - Mohammad Alizadeh
- Nutrition Research Center, Faculty of Nutrition, Tabriz University of Medical Sciences, Tabriz, Iran
| | - Safar Farajnia
- Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
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21
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Kuppusamy P, Paul Raj RD, Ilavenil S, Kaleeswaran B, Govindan N, Maniam GP, Ravikumar S. Evaluation of antihypercholesterolemic effect using Memecylon edule Roxb. ethanolic extract in cholesterol-induced Swiss albino mice. J Acute Med 2015. [DOI: 10.1016/j.jacme.2015.09.001] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.1] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/17/2022]
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22
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Goudarshivananavar BC, Vigneshwaran V, Somegowda M, Dharmappa KK, Pramod SN. Therapeutic potential of Polyalthia cerasoides stem bark extracts against oxidative stress and nociception. Anc Sci Life 2015; 35:70-8. [PMID: 26865738 PMCID: PMC4728867 DOI: 10.4103/0257-7941.171667] [Citation(s) in RCA: 3] [Impact Index Per Article: 0.3] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 01/24/2023] Open
Abstract
BACKGROUND Polyalthia cerasoides is a medicinal plant known for its ethnopharmacological importance. Despite this, investigation related to its therapeutic benefit is still unexplored. AIM To evaluate the stem bark extracts of Polyalthia cerasoides for pharmacological activities relating to inflammation, nociception and oxidative stress using in vivo and in vitro models. MATERIALS AND METHODS Pet ether, ethyl acetate and chloroform fractions of the stem bark were evaluated for anti-inflammatory activity by carrageenan-induced hind paw edema in rats. Anti-nociceptive activity in mice was assessed using thermally and chemically induced analgesic models. The free radical quenching potential of the extracts was initially analyzed using the in vitro DPPH photometric assay, Hydroxyl radical scavenging and Lipid Peroxidation assays. Then modulatory effect of the extracts on in vivo antioxidant system was evaluated by carbon tetrachloride induced hepatotoxicity and subsequent measurements of antioxidant enzymes such as Superoxide dismutase, Catalase and Peroxidase from the liver homogenate. RESULTS Among the tested fractions, ethyl acetate extract had substantially inhibited the inflammation by 68.5% that was induced by subcutaneous carrageenan injection whereas pet ether and chloroform extract showed only minimal inhibitory effect. Investigation of the anti-nociceptive activity revealed that the ethyl acetate fractions had significantly repressed the algesia in both the analgesic experimental models. In vitro and in vivo individual antioxidant assays demonstrated that the ethyl acetate fraction has strong free radical quenching potential which also restores the endogenous hepatic enzymes. CONCLUSION The ethyl acetate fraction enriched with flavinoids and steroids from Polyalthia cerasoides stem bark has potent bioactivity to combat inflammation, ROS and pain. This needs further characterization for potential therapeutic applications.
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Affiliation(s)
- B. C. Goudarshivananavar
- Department of Studies in Chemistry, Sahyadri Science College (Autonomous), Kuvempu University, Shimoga, Karnataka, India
| | - V. Vigneshwaran
- Department of Studies and Research in Biochemistry, Laboratory of Immunomodulation and Inflammation Biology, Sahyadri Science College (Autonomous), Kuvempu University, Shimoga, Karnataka, India
| | - Madhusudana Somegowda
- Department of Studies and Research in Biochemistry, Laboratory of Immunomodulation and Inflammation Biology, Sahyadri Science College (Autonomous), Kuvempu University, Shimoga, Karnataka, India
| | - Kattepura K. Dharmappa
- Department of Studies in Biochemistry, PG Centre of Mangalore University, Madikeri, Karnataka, India
| | - Siddanakoppalu N. Pramod
- Department of Studies and Research in Biochemistry, Laboratory of Immunomodulation and Inflammation Biology, Sahyadri Science College (Autonomous), Kuvempu University, Shimoga, Karnataka, India
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Gumus ZP, Guler E, Demir B, Barlas FB, Yavuz M, Colpankan D, Senisik AM, Teksoz S, Unak P, Coskunol H, Timur S. Herbal infusions of black seed and wheat germ oil: Their chemical profiles, in vitro bio-investigations and effective formulations as Phyto-Nanoemulsions. Colloids Surf B Biointerfaces 2015; 133:73-80. [PMID: 26087391 DOI: 10.1016/j.colsurfb.2015.05.044] [Citation(s) in RCA: 22] [Impact Index Per Article: 2.2] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 03/19/2015] [Revised: 05/21/2015] [Accepted: 05/26/2015] [Indexed: 01/11/2023]
Abstract
The reported studies related to black seed oil (BSO) and wheat germ oil (WGO) have illustrated that they have a wide range of biological activities. Therefore, enhancing the amount of bio-active compounds that caused higher cell based anti-oxidative effect as well as cell proliferation, etc. in seed oils, infusion of crude plant material has been gained importance as a traditional technique. Herein, we accomplished the infusion of Calendula flowers that also contains many phyto-constituents into BSO and WGO. After the infusion of oils, the change of phytochemical amount was investigated and evaluated according to the oils by chromatography, radical scavenging activity. Subsequently, for investigating the biological impact upon live cells, cytotoxicity, cell-based antioxidant capacity, wound healing and radioprotective activity were tested with monkey kidney fibroblast like cells (Vero) and HaCaT keratinocytes. In vitro cell based experiments (wound healing and radioprotective activity) confirmed that Calendula infused BSO and WGO have greater bio-activity when compared to those plain forms. The herbal oils prepared with an effective extraction technique were incorporated into nanoemulsion systems which will be then called as 'Phyto-Nanoemulsion'. After herbal oil biomolecules were encapsulated into nanoemulsion based delivery systems, the designed formulations were investigated in terms of biological activities. In conclusion, these preparations could be a good candidate as a part of dermal cosmetic products or food supplements which have the therapeutic efficiency, especially after radio- or chemotherapy.
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Affiliation(s)
- Z Pinar Gumus
- Institute of Drug Abuse Toxicology & Pharmaceutical Sciences, Ege University, 35100 Bornova, Izmir, Turkey
| | - Emine Guler
- Institute of Drug Abuse Toxicology & Pharmaceutical Sciences, Ege University, 35100 Bornova, Izmir, Turkey; Faculty of Science, Biochemistry Department, Ege University, 35100 Bornova, Izmir, Turkey
| | - Bilal Demir
- Faculty of Science, Biochemistry Department, Ege University, 35100 Bornova, Izmir, Turkey
| | - F Baris Barlas
- Faculty of Science, Biochemistry Department, Ege University, 35100 Bornova, Izmir, Turkey
| | - Murat Yavuz
- Faculty of Science, Chemistry Department, Dicle University, 21280 Diyarbakir, Turkey
| | - Dilara Colpankan
- Faculty of Science, Biochemistry Department, Ege University, 35100 Bornova, Izmir, Turkey
| | | | - Serap Teksoz
- Institute of Nuclear Sciences, Ege University, 35100 Bornova, Izmir, Turkey
| | - Perihan Unak
- Institute of Nuclear Sciences, Ege University, 35100 Bornova, Izmir, Turkey
| | - Hakan Coskunol
- Institute of Drug Abuse Toxicology & Pharmaceutical Sciences, Ege University, 35100 Bornova, Izmir, Turkey; School of Medicine, Department of Psychiatry, Ege University, 35100 Bornova, Izmir, Turkey; Ege LS, Ege University, 35100 Bornova, Izmir, Turkey
| | - Suna Timur
- Institute of Drug Abuse Toxicology & Pharmaceutical Sciences, Ege University, 35100 Bornova, Izmir, Turkey; Faculty of Science, Biochemistry Department, Ege University, 35100 Bornova, Izmir, Turkey.
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Hanczakowska E, Świątkiewicz M, Grela ER. Effect of dietary inclusion of a herbal extract mixture and different oils on pig performance and meat quality. Meat Sci 2015; 108:61-6. [PMID: 26047978 DOI: 10.1016/j.meatsci.2015.05.020] [Citation(s) in RCA: 39] [Impact Index Per Article: 3.9] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 07/29/2014] [Revised: 05/15/2015] [Accepted: 05/19/2015] [Indexed: 11/25/2022]
Abstract
The aim of the study was to evaluate the effect of a herbal extract mixture on pig performance and meat quality. The experiment was performed on 60 fatteners (60±0.5-112±2.0kg). Group I (control) was fed with standard feed; groups II and III received the same feed supplemented with 150mg BHT or 500mg of a herbal extract mixture (sage, nettle, lemon balm and coneflower) per kg of feed, respectively. In each group, half of the animals received 4% rapeseed oil, the other half soybean oil. The herbal extracts had no effect on animal performance but significantly improved meat oxidative stability, lowered cholesterol and TI index and increased PUFA content in meat. Slight differences between animals fed with rapeseed or soybean oils were observed. Gilt meat had significantly better (P≤0.01) AI, TI, and h/H indices than barrow meat. It was concluded that herbal extracts have a beneficial effect on pork health-promoting properties due to changes in lipid fraction.
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Affiliation(s)
- Ewa Hanczakowska
- Department of Animal Nutrition and Feed Science, National Research Institute of Animal Production, Krakowska 1 st., 32-083 Balice, Poland.
| | - Małgorzata Świątkiewicz
- Department of Animal Nutrition and Feed Science, National Research Institute of Animal Production, Krakowska 1 st., 32-083 Balice, Poland
| | - Eugeniusz R Grela
- Institute of Animal Nutrition and Bromatology, University of Life Sciences in Lublin, Akademicka 13 st., 20-950 Lublin, Poland
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Heshmati J, Namazi N, Memarzadeh MR, Taghizadeh M, Kolahdooz F. Nigella sativa oil affects glucose metabolism and lipid concentrations in patients with type 2 diabetes: A randomized, double-blind, placebo-controlled trial. Food Res Int 2015. [DOI: 10.1016/j.foodres.2015.01.030] [Citation(s) in RCA: 31] [Impact Index Per Article: 3.1] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 10/24/2022]
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Qujeq D, Tatar M, Feizi F, Parsian H, Halalkhor S. Urtica dioica Effect on Malonyl-CoA Decarboxylase. AVICENNA JOURNAL OF MEDICAL BIOCHEMISTRY 2014. [DOI: 10.17795/ajmb-18782] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/25/2022] Open
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Iqbal MS, Ghafoor A, Ullah I, Ahmad H. Quantification and Compositional Diversity of Fatty Acid Methyl Esters Profile in Nigella sativa L. Germplasm. J AM OIL CHEM SOC 2014. [DOI: 10.1007/s11746-014-2535-x] [Citation(s) in RCA: 5] [Impact Index Per Article: 0.5] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/25/2022]
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Andritoiu CV, Ochiuz L, Andritoiu V, Popa M. Effect of apitherapy formulations against carbon tetrachloride-induced toxicity in Wistar rats after three weeks of treatment. Molecules 2014; 19:13374-91. [PMID: 25178061 PMCID: PMC6270670 DOI: 10.3390/molecules190913374] [Citation(s) in RCA: 11] [Impact Index Per Article: 1.0] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 03/24/2014] [Revised: 08/19/2014] [Accepted: 08/21/2014] [Indexed: 11/16/2022] Open
Abstract
The human body is exposed nowadays to increasing attacks by toxic compounds in polluted air, industrially processed foods, alcohol and drug consumption that increase liver toxicity, leading to more and more severe cases of hepatic disorders. The present paper aims to evaluate the influence of the apitherapy diet in Wistar rats with carbon tetrachloride-induced hepatotoxicity, by analyzing the biochemical determinations (enzymatic, lipid and protein profiles, coagulation parameters, minerals, blood count parameters, bilirubin levels) and histopathological changes at the level of liver, spleen and pancreas. The experiment was carried out on six groups of male Wistar rats. Hepatic lesions were induced by intraperitoneal injection of carbon tetrachloride (dissolved in paraffin oil, 10% solution). Two mL per 100 g were administered, every 2 days, for 2 weeks. Hepatoprotection was achieved with two apitherapy diet formulations containing honey, pollen, propolis, Apilarnil, with/without royal jelly. Biochemical results reveal that the two apitherapy diet formulations have a positive effect on improving the enzymatic, lipid, and protein profiles, coagulation, mineral and blood count parameters and bilirubin levels. The histopathological results demonstrate the benefits of the two apitherapy diet formulations on reducing toxicity at the level of liver, spleen and pancreas in laboratory animals.
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Affiliation(s)
| | - Lacramioara Ochiuz
- Grigore T. Popa University of Medicine and Pharmacy of Iasi, Department of Pharmaceutical Technology, Faculty of Pharmacy, Universitatii Street, 16, Iasi 700115, Romania.
| | - Vasile Andritoiu
- Apitherapy Medical Center, Balanesti, Nr. 336-337, Gorj 217036, Romania.
| | - Marcel Popa
- Gheorghe Asachi Technical University of Iasi, Department of Natural and Synthetic Polymers, Iasi 700050, Romania.
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Emeka PM, Badger-Emeka LI, Eneh CM, Khan TM. Dietary supplementation of chloroquine with nigella sativa seed and oil extracts in the treatment of malaria induced in mice with plasmodium berghei. Pharmacogn Mag 2014; 10:S357-62. [PMID: 24991115 PMCID: PMC4078332 DOI: 10.4103/0973-1296.133282] [Citation(s) in RCA: 4] [Impact Index Per Article: 0.4] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 07/27/2013] [Revised: 10/10/2013] [Accepted: 05/28/2014] [Indexed: 01/23/2023] Open
Abstract
BACKGROUND The aim of the study was to investigate the effects of dietary combination of Nigella sativa seed and oil extracts with chloroquine (CQ), and how these combinations enhance CQ efficacy in mice infected with Plasmodium berghei and their survival rates. MATERIALS AND METHODS Chloroquine sensitive P. berghei, NK65 strain was used for the study. This was passaged intraperitoneally into albino mice with a 0.2ml standard inoculum consisting of 10(6) parasitized erythrocyte suspension in phosphate buffer solution (PBS). Parasitaemia was ascertained by microscopical examination of blood films under oil immersion at X100 magnification. RESULTS Nigella sativa seed in feed (NSSF), NSSF + CQ on day 4, produced 86.1% and 86.0% suppression respectively, while Nigella sativa oil extract in feed (NSOF) and in combination with CQ had 86.0% and 99.9% suppression respectively. The degree of suppression with the combination was significantly higher compared to CQ alone (P < 0.001) (36.1%). Complete parasitaemia clearance was obtained on the 20(th) and 15(th) day of treatment for NSSF, NSSF + CQ respectively, while that for NSOF and NSOF + CQ was on days 26 and 12 respectively. For CQ parasite clearance was 12 days with treatment. Also, the combinastion of 10 mg/kg Nigella sativa oil treatment injected intraperitoneally with oral CQ produced very significant parasite suppression (P < 0.0001) (93%). Survival rate in NSSF and NSOF and in combination with CQ groups was 100 and 60.0% for CQ alone. CONCLUSIONS This study shows that the use of Nigella sativa seed and oil extract as dietary supplements in combination with CQ has a potential in enhancing the efficacy of CQ and could be of benefit in management of malaria.
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Affiliation(s)
- Promise Madu Emeka
- Department of Pharmaceutical Sciences, College of Clinical Pharmacy, King Faisal University, Hofuf, Kingdom of Saudi Arabia
| | - Lorina Ineta Badger-Emeka
- Department of Biomedical Sciences, College of Medicine, King Faisal University, Hofuf, Kingdom of Saudi Arabia
| | | | - Tahir Mahmood Khan
- Department of Pharmacy Practice, School of Pharmacy, Monash University Malaysia, Selangor 47500, Malaysia
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Anti-inflammatuar and anti-oxidative effects of Nigella sativa L.: 18FDG-PET imaging of inflammation. Mol Biol Rep 2014; 41:2827-34. [DOI: 10.1007/s11033-014-3137-2] [Citation(s) in RCA: 23] [Impact Index Per Article: 2.1] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 05/30/2013] [Accepted: 01/11/2014] [Indexed: 11/26/2022]
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31
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Nigella sativa seed extract: 1. Enhancement of sheep macrophage immune functions in vitro. Res Vet Sci 2013; 95:437-43. [DOI: 10.1016/j.rvsc.2013.02.015] [Citation(s) in RCA: 15] [Impact Index Per Article: 1.3] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 05/05/2012] [Revised: 12/27/2012] [Accepted: 02/22/2013] [Indexed: 11/21/2022]
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Vanamala J, Kester AC, Heuberger AL, Reddivari L. Mitigation of obesity-promoted diseases by Nigella sativa and thymoquinone. PLANT FOODS FOR HUMAN NUTRITION (DORDRECHT, NETHERLANDS) 2012; 67:111-119. [PMID: 22477645 DOI: 10.1007/s11130-012-0279-z] [Citation(s) in RCA: 17] [Impact Index Per Article: 1.3] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 05/31/2023]
Abstract
Obesity is closely associated with increased incidence of cardiovascular diseases, cancer, insulin resistance, and immune dysfunction, and thus obesity-mitigation strategies should take into account these secondary pathologies in addition to promoting weight loss. Recent studies indicate that black cumin (Nigella sativa) has cardio-protective, anti-cancer, anti-diabetic, antioxidant, and immune-modulatory properties. While black cumin and/or its major bioactive constituent, thymoquinone have demonstrated bioactivity in a variety of disease models, the mechanisms of action are largely unknown. Given the growing interest in and the use of functional foods and nutraceuticals, as well as the increase in obesity and chronic diseases worldwide, further research into the therapeutic/preventive effects of black cumin may be beneficial.
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Affiliation(s)
- Jairam Vanamala
- Department of Food Science and Human Nutrition, Colorado State University, 1571 Campus Mail, 206 Gifford Building, Fort Collins, CO 80523, USA.
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Application, chemical composition, and standardization of nettle raw material and related drugs (Review). Pharm Chem J 2012. [DOI: 10.1007/s11094-012-0690-7] [Citation(s) in RCA: 4] [Impact Index Per Article: 0.3] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 12/08/2022]
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Genc Z, Yarat A, Tunali-Akbay T, Sener G, Cetinel S, Pisiriciler R, Caliskan-Ak E, Altıntas A, Demirci B. The Effect of Stinging Nettle (Urtica dioica) Seed Oil on Experimental Colitis in Rats. J Med Food 2011; 14:1554-61. [DOI: 10.1089/jmf.2011.0028] [Citation(s) in RCA: 10] [Impact Index Per Article: 0.7] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 01/06/2023] Open
Affiliation(s)
- Zeynep Genc
- Department of Biochemistry, Marmara University, Istanbul, Turkey
| | - Aysen Yarat
- Department of Biochemistry, Marmara University, Istanbul, Turkey
| | | | - Goksel Sener
- Department of Pharmacology, Faculty of Pharmacy, Marmara University, Istanbul, Turkey
| | - Sule Cetinel
- Department of Histology and Embryology, Faculty of Medicine, Marmara University, Istanbul, Turkey
| | - Rabia Pisiriciler
- Department of Histology and Embryology, Faculty of Dentistry, Marmara University, Istanbul, Turkey
| | - Esin Caliskan-Ak
- Department of Histology and Embryology, Faculty of Dentistry, Marmara University, Istanbul, Turkey
| | - Ayhan Altıntas
- Department of Pharmacognosy, Faculty of Pharmacy, Anadolu University, Eskisehir, Turkey
| | - Betul Demirci
- Department of Pharmacognosy, Faculty of Pharmacy, Anadolu University, Eskisehir, Turkey
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Çetin M, Yurtseven S, Şengül T, Sögüt B. Effect of Black Seed Extract (Nigella sativa) on Growth Performance, Blood Parameters, Oxidative Stress and DNA Damage of Partridges. JOURNAL OF APPLIED ANIMAL RESEARCH 2011. [DOI: 10.1080/09712119.2008.9706955] [Citation(s) in RCA: 2] [Impact Index Per Article: 0.1] [Reference Citation Analysis] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 10/15/2022]
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Katiyar D, Singh B, Lall AM, Haldar C. Efficacy of chitooligosaccharides for the management of diabetes in alloxan induced mice: A correlative study with antihyperlipidemic and antioxidative activity. Eur J Pharm Sci 2011; 44:534-43. [DOI: 10.1016/j.ejps.2011.09.015] [Citation(s) in RCA: 37] [Impact Index Per Article: 2.6] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 06/25/2011] [Revised: 09/10/2011] [Accepted: 09/15/2011] [Indexed: 11/28/2022]
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Effet antidiabétogène et cytoprotecteur de l’extrait butanolique de Ranunculus repens L. et de la quercétine sur un modèle expérimental de diabète alloxanique. ACTA ACUST UNITED AC 2011. [DOI: 10.1007/s10298-011-0651-4] [Citation(s) in RCA: 7] [Impact Index Per Article: 0.5] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/26/2022]
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Chand N, Durrani FR, Ahmad S, Khan A. Immunomodulatory and hepatoprotective role of feed-added Berberis lycium in broiler chicks. JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE 2011; 91:1737-1745. [PMID: 21480270 DOI: 10.1002/jsfa.4399] [Citation(s) in RCA: 9] [Impact Index Per Article: 0.6] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 10/21/2010] [Revised: 02/08/2011] [Accepted: 03/01/2011] [Indexed: 05/30/2023]
Abstract
BACKGROUND A large number of plants and their isolates have been shown to potentiate immunity. Some plants exert anti-inflammatory and anti-stress effects, others hepatoprotective activity. In this study, 320 1-day-old broiler chicks were randomly divided into four major groups A, B, C and D and fed rations supplemented with 0, 15, 20 and 22.5 g Berberis lycium kg⁻¹ ration respectively. Each group was further divided into two subgroups, one vaccinated against Newcastle disease (ND) and infectious bursal disease (IBD), the other non-vaccinated. Antibody titre against IBD and ND, relative weight of lymphoid organs, post-challenge morbidity and mortality, serum hepatic enzymes and total serum protein were observed. RESULTS Group C had higher anti-IBD and anti-ND antibody titres. Relative bursa weight in groups C and D was higher until day 28, but birds in group C performed better at later stages of examination. Relative spleen weight was highest in group C. During initial stages there was no effect on relative thymus weight, but at later stages the effect was significant. Groups C and D performed similarly in terms of relative thymus weight. The birds were challenged to field IBD through intramuscular injection at a dose rate of 0.5 mL per bird. Post-challenge morbidity was lowest in groups C and D, while treatment significantly (P < 0.001) affected mortality amongst affected (morbid) birds. Levels of serum alanine aminotransferase and alkaline phosphatase were lowest in group C. Serum protein was similar in all groups and in both vaccinated and non-vaccinated broiler chicks. CONCLUSION Berberis lycium added to feed at 20 g kg⁻¹ is effective in improving immunity against ND and IBD as well as liver function in broiler chicks.
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Affiliation(s)
- Naila Chand
- Department of Poultry Science, Agricultural University Peshawar, Peshawar, Pakistan
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Tasawar Z, Siraj Z, Ahmad N, Lashari MH. The Effects of Nigella sativa (Kalonji) on Lipid Profile in Patients with Stable Coronary Artery Disease in Multan, Pakistan. ACTA ACUST UNITED AC 2011. [DOI: 10.3923/pjn.2011.162.167] [Citation(s) in RCA: 38] [Impact Index Per Article: 2.7] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/15/2022]
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Ganie SA, Haq E, Masood A, Hamid A, Zargar MA. Antioxidant and protective effect of ethyl acetate extract of podophyllum hexandrum rhizome on carbon tetrachloride induced rat liver injury. EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE : ECAM 2011; 2011:238020. [PMID: 21394192 PMCID: PMC3025527 DOI: 10.1155/2011/238020] [Citation(s) in RCA: 26] [Impact Index Per Article: 1.9] [Reference Citation Analysis] [Abstract] [Track Full Text] [Download PDF] [Figures] [Subscribe] [Scholar Register] [Received: 08/30/2010] [Accepted: 11/25/2010] [Indexed: 11/21/2022]
Abstract
The antioxidant and hepatoprotective activities of ethyl acetate extract was carefully investigated by the methods of DPPH radical scavenging activity, Hydroxyl radical scavenging activity, Superoxide radical scavenging activity, Hydrogen peroxide radical scavenging activity and its Reducing power ability. All these in vitro antioxidant activities were concentration dependent which were compared with standard antioxidants such as BHT, α-tocopherol. The hepatoprotective potential of Podophyllum hexandrum extract was also evaluated in male Wistar rats against carbon tetrachloride (CCl(4))-induced liver damage. Pre-treated rats were given ethyl acetate extract at 20, 30 and 50 mg/kg dose prior to CCl(4) administration (1 ml/kg, 1:1 in olive oil). Rats pre-treated with Podophyllum hexandrum extract remarkably prevented the elevation of serum AST, ALT, LDH and liver lipid peroxides in CCl(4)-treated rats. Hepatic glutathione levels were significantly increased by the treatment with the extract in all the experimental groups. The extract at the tested doses also restored the levels of liver homogenate enzymes (glutathione peroxidase, glutathione reductase, superoxide dismutase and glutathione-S- transferase) significantly. This study suggests that ethyl acetate extract of P. hexandrum has a liver protective effect against CCl(4)-induced hepatotoxicity and possess in vitro antioxidant activities.
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Affiliation(s)
| | - Ehtishamul Haq
- Department of Biotechnology, University of Kashmir, Srinagar 190006, India
| | - Akbar Masood
- Department of Biochemistry, University of Kashmir, Srinagar 190006, India
| | - Abid Hamid
- Indian Institute of Integrative Medicine, Jammu (Council of Scientific and Industrial Research), India
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Yoruk O, Gur FO, Uyanik H, Yasar M, Mutlu V, Altas E, Baysal E, Taysi S. Antioxidant Effects of Nigella Sativa in the Treatment of Experimentally Induced Rhinosinusitis. ACTA ACUST UNITED AC 2010. [DOI: 10.3889/mjms.1857-5773.2010.0101] [Citation(s) in RCA: 14] [Impact Index Per Article: 0.9] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/05/2022]
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Cemek M, Aymelek F, Büyükokuroğlu ME, Karaca T, Büyükben A, Yilmaz F. Protective potential of Royal Jelly against carbon tetrachloride induced-toxicity and changes in the serum sialic acid levels. Food Chem Toxicol 2010; 48:2827-32. [PMID: 20637822 DOI: 10.1016/j.fct.2010.07.013] [Citation(s) in RCA: 76] [Impact Index Per Article: 5.1] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 04/01/2010] [Revised: 06/17/2010] [Accepted: 07/08/2010] [Indexed: 11/30/2022]
Abstract
Royal Jelly (RJ) is used in the Turkish folk medicine for the treatment of number of disorders. The present study describes the hepatoprotective and antioxidant activities of the RJ against carbon tetrachloride (CCl(4))-induced acute liver damage. Sprague-Dawley rats were used for the experiment. CCl(4) (0.8 ml/kg; s.c.) and RJ (50, 100, 200mg/kg; orally) were given every other day, for 20 days. Malondialdehyde, reduced glutathione in whole blood and tissues; ceruloplasmin, sialic acid, ascorbic acid, retinol, β-carotene and liver enzymes levels in serum were measured. Additionally, histopathological alterations in the liver were examined. RJ exerted the significant protective effect on liver damage as well as on oxidative stress induced by CCl(4), resulting in reduced lipid peroxidation and improved endogenous antioxidant defence systems. It also reduced the elevated levels of liver enzymes. Histopathological study further confirmed the hepatoprotective effect of RJ, when compared with the CCl(4) treated control groups. In conclusion, present study reveals biological evidence that supports the use of RJ in the treatment of chemical-induced hepatotoxicity.
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Affiliation(s)
- Mustafa Cemek
- Department of Chemistry, Biochemistry Division, Faculty of Sciences and Arts, Kocatepe University, Afyonkarahisar, Turkey
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Abdelmoaty MA, Ibrahim MA, Ahmed NS, Abdelaziz MA. Confirmatory studies on the antioxidant and antidiabetic effect of quercetin in rats. Indian J Clin Biochem 2010; 25:188-92. [PMID: 23105908 DOI: 10.1007/s12291-010-0034-x] [Citation(s) in RCA: 64] [Impact Index Per Article: 4.3] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 10/19/2022]
Abstract
Quercetin (QE), one of natural flavanoid group, was widely distributed as a secondary metabolite in plant kingdom. It has been believed that oxidative stress plays a role in the pathogenesis of diabetes mellitus (DM). The aim of the present study was the evaluation of possible effects of QE on blood glucose and antioxidant enzymes in experimental streptozotocin (STZ)-induced diabetes in rats. STZ was injected intraperitoneally with single dose of 50 mg/kg for diabetes induction. QE (15 mg/kg bw day, intraperitoneal (i.p.) injection) was injected for 3 days prior to STZ administration; these injections were continued to the end of the study (for 25 days). Glucose tolerance test and random plasma glucose were done for all animals. Cellular antioxidant enzymes such as glutathione peroxidase (GSHPx), superoxide dismutase (SOD) and catalase (CAT) activities were measured in pancreatic homogenates. Quercetin had no effect on plasma glucose level of normal animals but its pre- treatment was able to prevent diabetes induced by single intraperitoneal injection of streptozocintreated rats. Antioxidant enzyme activity significantly decreased in STZ induced diabetic group. QE treatment significantly increased the antioxidant enzyme activities. It could be concluded that quercetin, a flavonoid with antioxidant properties, exerting its beneficial antidiabetic effects.
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Affiliation(s)
- Mohamed Ahmed Abdelmoaty
- Department of Biochemistry, Sohag University, Sohag, Egypt ; Sohag Faculty of Medicine, Sohag University, Sohag, Egypt ; Department of Biochemistry, Sohag faculty of Medicine, Sohag University, Sohag, Egypt
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Kandis H, Karapolat S, Yildirim U, Saritas A, Gezer S, Memisogullari R. Effects of Urtica dioica on hepatic ischemia-reperfusion injury in rats. Clinics (Sao Paulo) 2010; 65:1357-61. [PMID: 21340227 PMCID: PMC3020349 DOI: 10.1590/s1807-59322010001200021] [Citation(s) in RCA: 21] [Impact Index Per Article: 1.4] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Received: 07/05/2010] [Accepted: 09/27/2010] [Indexed: 12/11/2022] Open
Abstract
OBJECTIVES To evaluate the effects of Urtica dioica on hepatic ischemia-reperfusion injury. METHODS Thirty adult male Wistar albino rats were divided into three groups: sham group (group 1), control group (group 2), and Urtica dioica group (group 3). All the rats were exposed to hepatic ischemia for 60 min, followed by 60 min of reperfusion. In group 2, a total of 2 ml/kg 0.9% saline solution was given intraperitoneally. In group 3, a total of 2 ml/kg Urtica dioica was given intraperitoneally. At the end of the procedure, liver tissue and blood samples were taken from all rats. Serum aspartate aminotransferase, alanine aminotransferase, lactate dehydrogenase, ceruloplasmin, catalase, paraoxonase, arylesterase, and lipid hydroperoxide levels were measured. Liver tissue histopathologies were also evaluated by light microscopy. RESULTS Serum aspartate aminotransferase, alanine aminotransferase and lactate dehydrogenase levels were significantly higher in group 2 than in group 1, and significantly lower in group 3 than in group 2. Also, group 2 had higher serum lipid hydroperoxides and ceruloplasmin levels but lower catalase, paraoxonase, and arylesterase levels than group 1. In group 3, serum lipid hydroperoxides and ceruloplasmin levels were significantly lower, and catalase, paraoxonase, and arylesterase levels were higher than those in group 2. Histopathological examination showed that liver tissue damage was significantly decreased in group 3 compared with group 2. CONCLUSIONS Urtica dioica has a protective effect on the liver in hepatic ischemia-reperfusion-injured rats.
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Affiliation(s)
- Hayati Kandis
- Department of Emergency Medicine, Faculty of Medicine, Duzce University, Duzce, Turkey.
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Jodynis-Liebert J, Adamska T, Ewertowska M, Bylka W, Matławska I. Aquilegia vulgaris extract attenuates carbon tetrachloride-induced liver fibrosis in rats. ACTA ACUST UNITED AC 2009; 61:443-51. [PMID: 19059770 DOI: 10.1016/j.etp.2008.10.007] [Citation(s) in RCA: 6] [Impact Index Per Article: 0.4] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 02/16/2006] [Revised: 03/07/2008] [Accepted: 10/23/2008] [Indexed: 10/21/2022]
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Al-Naqeeb G, Ismail M, S. Al-Zuba A. Fatty Acid Profile, α-Tocopherol Content and Total Antioxidant Activity of Oil Extracted from Nigella sativa Seeds. INT J PHARMACOL 2009. [DOI: 10.3923/ijp.2009.244.250] [Citation(s) in RCA: 22] [Impact Index Per Article: 1.4] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/15/2022]
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Golalipour MJ, Khori V. The protective activity of Urtica dioica leaves on blood glucose concentration and beta-cells in streptozotocin-diabetic rats. Pak J Biol Sci 2009; 10:1200-4. [PMID: 19069917 DOI: 10.3923/pjbs.2007.1200.1204] [Citation(s) in RCA: 22] [Impact Index Per Article: 1.4] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/15/2022]
Abstract
This study was done to determine the protective activity of the hydroalcholic extract of Urtica dioica leaves on Hyperglycemia and beta-cells in hyperglycemic rats. Thirty Wistar rats were allocated in groups of normal, Diabetic and treatment. Hyperglycemia in Rats induced by 80 mg kg(-1) streptozotocin. In treatment group, animals received hydroalcholic extract of Urtica dioica 100 mg kg(-1) day(-1) for five days, intraperitoneally and then hyperglycemia induced by streptozotocin. The blood glucose concentration was measured by using a Glucometer in 1st, 3rd and 5th weeks. In the end of 5th weeks the animals in each group were sacrificed by anesthesia and whole pancreas in three groups extracted and fixed in bouin's fluid and stained by chromealum hematoxiline-phloxine and beta cells were counted in three groups by Olympus microscope. Mean +/- SE of blood glucose concentrations in the end of fifth weeks were 99.4 +/-5.0, 454.7 +/- 34.5 and 303.6 +/- 100.6 in control, diabetic and treatment groups, respectively (p < 0.05). The percentages of beta-cells in control, diabetic and treatment groups were 73.6, 1.9 and 22.9%, respectively. The percentage of beta-cells in treatment group comparing with diabetic group was significant (p < 0.05). This study showed that the protective administration of hydroalcholic extract of Urtica dioica has hypoglycemic effect and protective activity of beta-cells of langerhans in hyperglycemic rats.
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Dehkordi FR, Kamkhah AF. Antihypertensive effect ofNigella sativaseed extract in patients with mild hypertension. Fundam Clin Pharmacol 2008; 22:447-52. [DOI: 10.1111/j.1472-8206.2008.00607.x] [Citation(s) in RCA: 111] [Impact Index Per Article: 6.5] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/26/2022]
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Celik I, Isik I. Determination of chemopreventive role of Foeniculum vulgare and Salvia officinalis infusion on trichloroacetic acid-induced increased serum marker enzymes lipid peroxidation and antioxidative defense systems in rats. Nat Prod Res 2008; 22:66-75. [PMID: 17999340 DOI: 10.1080/14786410701590426] [Citation(s) in RCA: 14] [Impact Index Per Article: 0.8] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 10/22/2022]
Abstract
Today's world is increasingly seeking ways to replace the synthetic drugs with the therapeutic power of natural products. This study was designed to investigate the protective effects of Foeniculum vulgare (FV) and Salvia officinalis (SO) waters infusions against carcinogen chemical trichloroacetic acid (TCA)-exposure in rats. The chemopreventive potential of the plant infusions were evaluated by measuring levels of serum marker enzymes [aspartate aminotransferase (AST), alanin aminotransferase (ALT), creatine phosphokinase (CPK), acid phosphatase (ACP), alkaline phosphatase (ALP) and lactate dehydrogenase (LDH)], antioxidant defense systems [Reduced glutathione (GSH), glutathione reductase (GR), superoxide dismutase (SOD), glutathione-S-transferase (GST) and catalase (CAT)] and lipid peroxidation level (Malondialdehyde = MDA) in various tissues of rats. Female Sprague-Dawley rats, weighing 150-200 g, were randomly allotted into four experimental groups. While the control group (A) received only natural spring water, the treatment B group (0.2% TCA) supplied with the drinking water containing 0.2% TCA, the treatment C (TCA + FV infusion) and D (TCA + SO infusion) groups drank the drinking water containing 0.2% TCA and 2.5% the plant grains and leaves ad libitum for 50 days during experiment. At the end of the 50 days experiment, TCA and the plant's infusions caused different affect on the serum marker enzymes, tissues antioxidant defense systems and lipid peroxidation against TCA-exposed in rats with comparison to those of TCA exposed and control rats. According to the results, both TCA and TCA + plants infusions caused a significant increase in serum AST, ALT and CPK activity. Non-enzymic antioxidant GSH level significantly increased in the brain whereas reduced in the erythrocytes and kidney of TCA + FV and TCA + SO as compared to TCA group and control. While MDA content slightly increased in tissues of TCA group in comparison to those of control, significantly decreased in the brain, liver and kidney of rats of TCA + FV and TCA + SO groups as compared to TCA group and control. Antioxidative enzyme activity such as CAT and SOD significantly increased in the brain, liver and kidney tissues of TCA induced group whereas reduced the same enzymes activities as compared to TCA group. The ancillary enzyme GR activity significantly depleted in the brain and kidney of TCA + FV and TCA + SO groups in comparison to those of TCA exposed and control rats. In addition, the drug metabolizing enzyme GST activity significantly declined in the brain and kidney of TCA + FV and TCA + SO groups in comparison to those of TCA exposed and control rats, whereas, also reduced in the liver of TCA + FV and TCA + SO groups in comparison to those of TCA exposed rats. It was concluded that the levels of serum marker enzymes were found not to be decreased in plants treated groups due to hepatic damage induced by TCA. Also the four antioxidant enzymes were found to be activated in different degrees following TCA treatment and declined the activation of the enzymes the plant infusions accompanied by significant reduction in MDA concentration in the tissues. The observations, along with changes, might suggest that the both FV and SO may possess antioxidant properties during the period of a 50-day protective exposure.
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Affiliation(s)
- Ismail Celik
- Faculty of Art and Science, Department of Biology, Yuzuncu Yil University, Van, Turkey.
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