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Sun B, Zheng C, Pan D, Shen L, Zhang W, Chen X, Wen Y, Shi Y. Using AuNPs-DNA Walker with Fluorophores Detects the Hepatitis Virus Rapidly. BIOSENSORS 2024; 14:370. [PMID: 39194599 DOI: 10.3390/bios14080370] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Grants] [Track Full Text] [Subscribe] [Scholar Register] [Received: 05/05/2024] [Revised: 07/19/2024] [Accepted: 07/23/2024] [Indexed: 08/29/2024]
Abstract
Viral hepatitis is a systemic infectious diseases caused by various hepatitis viruses, primarily leading to liver damage. It is widely prevalent worldwide, with hepatitis viruses categorized into five types: hepatitis A, B, C, D, and E, based on their etiology. Currently, the detection of hepatitis viruses relies on methods such as enzyme-linked immunosorbent assay (ELISA), immunoelectron microscopy to observe and identify viral particles, and in situ hybridization to detect viral DNA in tissues. However, these methods have limitations, including low sensitivity, high error rates in results, and potential false negative reactions due to occult serum infection conditions. To address these challenges, we have designed an AuNPs-DNA walker method that uses gold nanoparticles (AuNPs) and complementary DNA strands for detecting viral DNA fragments through a colorimetric assay and fluorescence detection. The DNA walker, attached to gold nanoparticles, comprises a long walking strand with a probe sequence bound and stem-loop structural strands featuring a modified fluorescent molecule at the 3' end, which contains the DNAzyme structural domain. Upon the addition of virus fragments, the target sequence binds to the probe chains. Subsequently, the long walking strand is released and continuously hybridizes with the stem-loop structural strand. The DNAzyme undergoes hydrolytical cleavage by Mg2+, breaking the stem-loop structural strand into linear single strands. As a result of these structural changes, the negative charge density in the solution decreases, weakening spatial repulsion and rapidly reducing the stability of the DNA walker. This leads to aggregation upon the addition of a high-salt solution, accompanied by a color change. Virus typing can be performed through fluorescence detection. The innovative method can detect DNA/RNA fragments with high specificity for the target sequence, reaching concentrations as low as 1 nM. Overall, our approach offers a more convenient and reliable method for the detection of hepatitis viruses.
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Affiliation(s)
- Baining Sun
- Bio-X Institutes, Key Laboratory for the Genetics of Developmental and Neuropsychiatric Disorders (Ministry of Education), Shanghai Jiao Tong University, Shanghai 200030, China
| | - Chenxiang Zheng
- Bio-X Institutes, Key Laboratory for the Genetics of Developmental and Neuropsychiatric Disorders (Ministry of Education), Shanghai Jiao Tong University, Shanghai 200030, China
| | - Dun Pan
- Bio-X Institutes, Key Laboratory for the Genetics of Developmental and Neuropsychiatric Disorders (Ministry of Education), Shanghai Jiao Tong University, Shanghai 200030, China
| | - Leer Shen
- Department of Infectious Diseases, Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200233, China
| | - Wan Zhang
- Bio-X Institutes, Key Laboratory for the Genetics of Developmental and Neuropsychiatric Disorders (Ministry of Education), Shanghai Jiao Tong University, Shanghai 200030, China
| | - Xiaohua Chen
- Department of Infectious Diseases, Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200233, China
| | - Yanqin Wen
- Bio-X Institutes, Key Laboratory for the Genetics of Developmental and Neuropsychiatric Disorders (Ministry of Education), Shanghai Jiao Tong University, Shanghai 200030, China
| | - Yongyong Shi
- Bio-X Institutes, Key Laboratory for the Genetics of Developmental and Neuropsychiatric Disorders (Ministry of Education), Shanghai Jiao Tong University, Shanghai 200030, China
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Fluorescence-based simultaneous dual oligo sensing of HCV genotypes 1 and 3 using magnetite nanoparticles. JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY. B, BIOLOGY 2022; 232:112463. [PMID: 35567883 DOI: 10.1016/j.jphotobiol.2022.112463] [Citation(s) in RCA: 2] [Impact Index Per Article: 0.7] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Subscribe] [Scholar Register] [Received: 01/25/2022] [Revised: 04/12/2022] [Accepted: 05/05/2022] [Indexed: 12/12/2022]
Abstract
Nucleic acid tests (NATs) have gained an important position in biosensing in the context of the increasing need to meet the stringent requirements for accurate diagnosis of infectious diseases with high sensitivity and selectivity. Recently, the development of new strategies towards multiplex detection of analytes in a single assay is gaining impetus since such an approach would lead to high throughput analysis, leading to substantial benefits in terms of time, infrastructure, labor, and cost. In this work, we demonstrate a facile fluorescence-based simultaneous dual oligo sensing of genotypes 1 and 3 by employing two target sequences (36-mers each) derived from the NS4B and NS5A regions of HCV genome, respectively. A set of 18-mer amine-tagged probes and another set of 18-mer fluorescently-labeled probes that were complementary to each half of the 36-mer target sequences were designed. The amine-tagged probes were immobilized over aldehyde-derivatized magnetite nanoparticles (NPs) via imine bond formation, which was characterized using X-ray photoelectron spectroscopy (XPS) and energy dispersive spectroscopy (EDS) mapping techniques. The successful hybridization between the two probes with their target followed by magnetic removal of the NPs from the solution enabled quantitative analysis of the target by measuring the fluorescence intensity of the residual concentration of the fluorescently-tagged probe. In this manner, the targets corresponding to genotypes 1 and 3 were simultaneously detected with the detection limit in the range of 10-15 nM. The current strategy can potentially be amalgamated with existing nanotechnology-based techniques towards multiplex oligo sensing of several pathogens.
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Babaei A, Pouremamali A, Rafiee N, Sohrabi H, Mokhtarzadeh A, de la Guardia M. Genosensors as an alternative diagnostic sensing approaches for specific detection of various certain viruses: a review of common techniques and outcomes. Trends Analyt Chem 2022; 155:116686. [PMID: 35611316 PMCID: PMC9119280 DOI: 10.1016/j.trac.2022.116686] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.3] [Reference Citation Analysis] [Abstract] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 12/08/2021] [Revised: 05/08/2022] [Accepted: 05/15/2022] [Indexed: 12/19/2022]
Abstract
Viral infections are responsible for the deaths of millions of people throughout the world. Since outbreak of highly contagious and mutant viruses such as contemporary sars-cov-2 pandemic, has challenged the conventional diagnostic methods, the entity of a thoroughly sensitive, specific, rapid and inexpensive detecting technique with minimum level of false-positivity or -negativity, is desperately needed more than any time in the past decades. Biosensors as minimized devices could detect viruses in simple formats. So far, various nucleic acid, immune- and protein-based biosensors were designed and tested for recognizing the genome, antigen, or protein level of viruses, respectively; however, nucleic acid-based sensing techniques, which is the foundation of constructing genosensors, are preferred not only because of their ultra-sensitivity and applicability in the early stages of infections but also for their ability to differentiate various strains of the same virus. To date, the review articles related to genosensors are just confined to particular pathogenic diseases; In this regard, the present review covers comprehensive information of the research progress of the electrochemical, optical, and surface plasmon resonance (SPR) genosensors that applied for human viruses' diseases detection and also provides a well description of viruses' clinical importance, the conventional diagnosis approaches of viruses and their disadvantages. This review would address the limitations in the current developments as well as the future challenges involved in the successful construction of sensing approaches with the functionalized nanomaterials and also allow exploring into core-research works regarding this area.
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Affiliation(s)
- Abouzar Babaei
- Department of Virology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
| | - Amir Pouremamali
- Department of Virology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
| | - Nastaran Rafiee
- Department of Virology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
| | - Hessamaddin Sohrabi
- Department of Analytical Chemistry, Faculty of Chemistry, University of Tabriz, Tabriz, Iran
| | - Ahad Mokhtarzadeh
- Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
| | - Miguel de la Guardia
- Department of Analytical Chemistry, University of Valencia, Dr. Moliner 50, 46100, Burjassot, Valencia, Spain
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Theerthagiri J, Lee SJ, Karuppasamy K, Park J, Yu Y, Kumari MLA, Chandrasekaran S, Kim HS, Choi MY. Fabrication strategies and surface tuning of hierarchical gold nanostructures for electrochemical detection and removal of toxic pollutants. JOURNAL OF HAZARDOUS MATERIALS 2021; 420:126648. [PMID: 34329090 DOI: 10.1016/j.jhazmat.2021.126648] [Citation(s) in RCA: 37] [Impact Index Per Article: 9.3] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 06/09/2021] [Revised: 07/10/2021] [Accepted: 07/12/2021] [Indexed: 05/20/2023]
Abstract
The intensive research on the synthesis and characterization of gold (Au) nanostructures has been extensively documented over the last decades. These investigations allow the researchers to understand the relationships between the intrinsic properties of Au nanostructures such as particle size, shape, morphology, and composition to synthesize the Au nano/hybrid nanostructures with novel physicochemical properties. By tuning the properties above, these nanostructures are extensively employed to detect and remove trace amounts of toxic pollutants from the environment. This review attempts to document the achievements and current progress in Au-based nanostructures, general synthetic and fabrication strategies and their utilization in electrochemical sensing and environmental remediation applications. Additionally, the applications of Au nanostructures (e.g., as adsorbents, sensing platforms, catalysts, and electrodes) and advancements in the field of electrochemical sensing of different target analytes (e.g., proteins, nucleic acids, heavy metals, small molecules, and antigens) are summarized. The literature survey concludes the existing methods for the detection of toxic contaminants at various concentration levels. Finally, the existing challenges and future research directions on electrochemical sensing and degradation of toxic contaminants using Au nanostructures are defined.
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Affiliation(s)
- Jayaraman Theerthagiri
- Core-Facility Center for Photochemistry & Nanomaterials, Department of Chemistry (BK21 FOUR), Research Institute of Natural Sciences, Gyeongsang National University, Jinju 52828, South Korea
| | - Seung Jun Lee
- Core-Facility Center for Photochemistry & Nanomaterials, Department of Chemistry (BK21 FOUR), Research Institute of Natural Sciences, Gyeongsang National University, Jinju 52828, South Korea
| | - K Karuppasamy
- Division of Electronics and Electrical Engineering, Dongguk University-Seoul, Seoul 04620, Republic of Korea
| | - Juhyeon Park
- Core-Facility Center for Photochemistry & Nanomaterials, Department of Chemistry (BK21 FOUR), Research Institute of Natural Sciences, Gyeongsang National University, Jinju 52828, South Korea
| | - Yiseul Yu
- Core-Facility Center for Photochemistry & Nanomaterials, Department of Chemistry (BK21 FOUR), Research Institute of Natural Sciences, Gyeongsang National University, Jinju 52828, South Korea
| | - M L Aruna Kumari
- Department of Chemistry, M.S. Ramaiah College of Arts, Science and Commerce, Bengaluru 560054, India
| | - Sivaraman Chandrasekaran
- Center of Excellence in Environmental Studies, King Abdulaziz University, Jeddah 21589, Saudi Arabia
| | - Hyun-Seok Kim
- Division of Electronics and Electrical Engineering, Dongguk University-Seoul, Seoul 04620, Republic of Korea
| | - Myong Yong Choi
- Core-Facility Center for Photochemistry & Nanomaterials, Department of Chemistry (BK21 FOUR), Research Institute of Natural Sciences, Gyeongsang National University, Jinju 52828, South Korea.
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Mohammed AS, Balapure A, Khan AA, Khaja MN, Ganesan R, Dutta JR. Genotyping simplified: rationally designed antisense oligonucleotide-mediated PCR amplification-free colorimetric sensing of viral RNA in HCV genotypes 1 and 3. Analyst 2021; 146:4767-4774. [PMID: 34231566 DOI: 10.1039/d1an00590a] [Citation(s) in RCA: 3] [Impact Index Per Article: 0.8] [Reference Citation Analysis] [Abstract] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 12/15/2022]
Abstract
Molecular diagnosis of viral genotyping devoid of polymerase chain reaction (PCR) amplification in clinical cohorts has hitherto been challenging. Here we present a simplified molecular diagnostic strategy for direct genotyping of hepatitis C virus (HCV) 1 and 3 (prevalent worldwide) using a combination of rationally designed genotype-specific antisense oligonucleotides (ASOs) and plasmonic gold nanoparticles. The ASOs specific to genotypes 1 and 3 have been designed from the nonstructural region 5A (NS5A) of the viral genome using the ClustalW multiple sequence alignment tool. A total of 79 clinical samples including 18 HCV genotype 1, 18 HCV genotype 3, one HIV positive, one HBV positive, and 41 healthy controls have been tested against both the designed ASOs. The study reveals 100% specificity and sensitivity with the employed samples and thereby opens up new avenues for PCR-free direct genotyping of other viruses as well, through the rational design of ASOs.
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Affiliation(s)
- Almas Shamaila Mohammed
- Department of Biological Sciences, BITS Pilani Hyderabad Campus, Jawahar Nagar, Kapra Mandal, Medchal, Hyderabad-500078, India.
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Teengam P, Nisab N, Chuaypen N, Tangkijvanich P, Vilaivan T, Chailapakul O. Fluorescent paper-based DNA sensor using pyrrolidinyl peptide nucleic acids for hepatitis C virus detection. Biosens Bioelectron 2021; 189:113381. [PMID: 34090155 DOI: 10.1016/j.bios.2021.113381] [Citation(s) in RCA: 23] [Impact Index Per Article: 5.8] [Reference Citation Analysis] [Abstract] [Key Words] [Journal Information] [Subscribe] [Scholar Register] [Received: 02/28/2021] [Revised: 05/07/2021] [Accepted: 05/17/2021] [Indexed: 12/17/2022]
Abstract
A novel fluorescent paper-based DNA sensor employing a highly specific pyrrolidinyl peptide nucleic acid (acpcPNA) probe was developed for the sensitive and selective detection of hepatitis C virus (HCV). The acpcPNA was covalently immobilized onto partially oxidized cellulose paper via reductive alkylation between the amine and the aldehyde groups. The fluorescence-based detection was performed by monitoring the fluorescence signal response of a fluorescent dye that selectively binds to the single-strand region of the DNA target over the PNA probe employing a custom-made portable fluorescent camera gadget in combination with a smartphone camera. Under the optimal conditions, a linear relationship between the fluorescence change in the green channel and the amount of HCV DNA from 5 to 100 pmol with a correlation coefficient of 0.9956, and the limit of detection of 5 pmol were obtained for short synthetic oligonucleotides. The acpcPNA probe exhibited very high selectivity for the complementary oligonucleotides over the single-base-mismatched, two-base-mismatched, and non-complementary DNA targets. Benefitting from the signal amplification achieved through the numerous binding sites for the dye provided by the overhanging tail of long ssDNA target sequences, this system was successfully applied to detect the HCV complementary DNA (cDNA) obtained from clinical samples with satisfactory results. The proposed fluorescent paper-based sensor demonstrated a great potential to be used as a low-cost, simple, label-free, sensitive, and selective DNA sensor for point-of-care applications.
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Affiliation(s)
- Prinjaporn Teengam
- Electrochemistry and Optical Spectroscopy Center of Excellence, Department of Chemistry, Faculty of Science, Chulalongkorn University, Pathumwan, Bangkok, 10330, Thailand
| | - Narathorn Nisab
- Electrochemistry and Optical Spectroscopy Center of Excellence, Department of Chemistry, Faculty of Science, Chulalongkorn University, Pathumwan, Bangkok, 10330, Thailand
| | - Natthaya Chuaypen
- Center of Excellence in Hepatitis and Liver Cancer, Department of Biochemistry, Faculty of Medicine, Chulalongkorn University, Pathumwan, Bangkok, 10330, Thailand
| | - Pisit Tangkijvanich
- Center of Excellence in Hepatitis and Liver Cancer, Department of Biochemistry, Faculty of Medicine, Chulalongkorn University, Pathumwan, Bangkok, 10330, Thailand
| | - Tirayut Vilaivan
- Organic Synthesis Research Unit, Department of Chemistry, Faculty of Science, Chulalongkorn University, Pathumwan, Bangkok, 10330, Thailand.
| | - Orawon Chailapakul
- Electrochemistry and Optical Spectroscopy Center of Excellence, Department of Chemistry, Faculty of Science, Chulalongkorn University, Pathumwan, Bangkok, 10330, Thailand.
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Goud KY, Reddy KK, Khorshed A, Kumar VS, Mishra RK, Oraby M, Ibrahim AH, Kim H, Gobi KV. Electrochemical diagnostics of infectious viral diseases: Trends and challenges. Biosens Bioelectron 2021; 180:113112. [PMID: 33706158 PMCID: PMC7921732 DOI: 10.1016/j.bios.2021.113112] [Citation(s) in RCA: 52] [Impact Index Per Article: 13.0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 01/05/2021] [Revised: 02/06/2021] [Accepted: 02/22/2021] [Indexed: 02/07/2023]
Abstract
Infectious diseases caused by viruses can elevate up to undesired pandemic conditions affecting the global population and normal life function. These in turn impact the established world economy, create jobless situations, physical, mental, emotional stress, and challenge the human survival. Therefore, timely detection, treatment, isolation and prevention of spreading the pandemic infectious diseases not beyond the originated town is critical to avoid global impairment of life (e.g., Corona virus disease - 2019, COVID-19). The objective of this review article is to emphasize the recent advancements in the electrochemical diagnostics of twelve life-threatening viruses namely - COVID-19, Middle east respiratory syndrome (MERS), Severe acute respiratory syndrome (SARS), Influenza, Hepatitis, Human immunodeficiency virus (HIV), Human papilloma virus (HPV), Zika virus, Herpes simplex virus, Chikungunya, Dengue, and Rotavirus. This review describes the design, principle, underlying rationale, receptor, and mechanistic aspects of sensor systems reported for such viruses. Electrochemical sensor systems which comprised either antibody or aptamers or direct/mediated electron transfer in the recognition matrix were explicitly segregated into separate sub-sections for critical comparison. This review emphasizes the current challenges involved in translating laboratory research to real-world device applications, future prospects and commercialization aspects of electrochemical diagnostic devices for virus detection. The background and overall progress provided in this review are expected to be insightful to the researchers in sensor field and facilitate the design and fabrication of electrochemical sensors for life-threatening viruses with broader applicability to any desired pathogens.
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Affiliation(s)
- K Yugender Goud
- Department of NanoEngineering, University of California San Diego, La Jolla, CA, 92093, USA.
| | - K Koteshwara Reddy
- Smart Living Innovation Technology Centre, Department of Energy Science and Technology, Myongji University, Yongin, Gyeonggi-do, 17058, Republic of Korea.
| | - Ahmed Khorshed
- Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Sohag University, Sohag, 82524, Egypt.
| | - V Sunil Kumar
- Department of Chemistry, National Institute of Technology Warangal, Telangana, 506004, India
| | - Rupesh K Mishra
- Department of NanoEngineering, University of California San Diego, La Jolla, CA, 92093, USA
| | - Mohamed Oraby
- Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Sohag University, Sohag, 82524, Egypt
| | - Alyaa Hatem Ibrahim
- Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Sohag University, Sohag, 82524, Egypt
| | - Hern Kim
- Smart Living Innovation Technology Centre, Department of Energy Science and Technology, Myongji University, Yongin, Gyeonggi-do, 17058, Republic of Korea.
| | - K Vengatajalabathy Gobi
- Department of Chemistry, National Institute of Technology Warangal, Telangana, 506004, India.
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Ba Y, Zhang J, Sun Y, Liu Y, Yang H, Kong J. Novel fluorescent biosensor for carcinoembryonic antigen determination via atom transfer radical polymerization with a macroinitiator. NEW J CHEM 2021. [DOI: 10.1039/d0nj05822j] [Citation(s) in RCA: 2] [Impact Index Per Article: 0.5] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 12/11/2022]
Abstract
A novel fluorescence method for CEA via β-CD and BIBB-initiated atom transfer radical polymerization (ATRP) was reported.
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Affiliation(s)
- Yanyan Ba
- Pharmacy College
- Henan University of Traditional Chinese Medicine
- Zhengzhou 450046
- P. R. China
| | - Jingyu Zhang
- Pharmacy College
- Henan University of Traditional Chinese Medicine
- Zhengzhou 450046
- P. R. China
| | - Yuzhi Sun
- Pharmacy College
- Henan University of Traditional Chinese Medicine
- Zhengzhou 450046
- P. R. China
| | - Yanju Liu
- Pharmacy College
- Henan University of Traditional Chinese Medicine
- Zhengzhou 450046
- P. R. China
| | - Huaixia Yang
- Pharmacy College
- Henan University of Traditional Chinese Medicine
- Zhengzhou 450046
- P. R. China
| | - Jinming Kong
- School of Environmental and Biological Engineering
- Nanjing University of Science and Technology
- Nanjing 210094
- P. R. China
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9
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Ngo DB, Chaibun T, Yin LS, Lertanantawong B, Surareungchai W. Electrochemical DNA detection of hepatitis E virus genotype 3 using PbS quantum dot labelling. Anal Bioanal Chem 2020; 413:1027-1037. [PMID: 33236225 DOI: 10.1007/s00216-020-03061-1] [Citation(s) in RCA: 9] [Impact Index Per Article: 1.8] [Reference Citation Analysis] [Abstract] [Key Words] [Journal Information] [Subscribe] [Scholar Register] [Received: 09/24/2020] [Revised: 11/06/2020] [Accepted: 11/10/2020] [Indexed: 12/30/2022]
Abstract
The aim of this study was to develop a highly specific electrochemical DNA sensor using functionalized lead sulphide (PbS) quantum dots for hepatitis E virus genotype 3 (HEV3) DNA target detection. Functionalized-PbS quantum dots (QDs) were used as an electrochemical label for the detection of HEV3-DNA target by the technique of square wave anodic stripping voltammetry (SWASV). The functionalized-PbS quantum dots were characterized by UV-vis, FTIR, XRD, TEM and zeta potential techniques. As-prepared, functionalized-PbS quantum dots have an average size of 4.15 ± 1.35 nm. The detection platform exhibited LOD and LOQ values of 1.23 fM and 2.11 fM, respectively. HEV3-DNA target spiked serum is also reported.Graphical abstract.
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Affiliation(s)
- Duy Ba Ngo
- School of Bioresources and Technology, King Mongkut's University of Technology Thonburi (KMUTT), Bangkhuntien-Chaitalay Road, Bangkok, 10150, Thailand
| | - Thanyarat Chaibun
- Department of Biomedical Engineering, Faculty of Engineering, Mahidol University, Phutthamonthon, Nakhon Pathom, 73170, Thailand
| | - Lee Su Yin
- Department of Biotechnology, Faculty of Applied Sciences, AIMST University, Jalan, Bukit Air Nasi, 08100, Bedong, Kedah, Malaysia
| | - Benchaporn Lertanantawong
- Department of Biomedical Engineering, Faculty of Engineering, Mahidol University, Phutthamonthon, Nakhon Pathom, 73170, Thailand.
| | - Werasak Surareungchai
- School of Bioresources and Technology, King Mongkut's University of Technology Thonburi (KMUTT), Bangkhuntien-Chaitalay Road, Bangkok, 10150, Thailand. .,Nanoscience and Nanotechnology Graduate Program, Faculty of Science, King Mongkut's University of Technology Thonburi, Pracha Uthit Rd, Bangkok, 10140, Thailand.
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Roohizadeh A, Ghaffarinejad A, Salahandish R, Omidinia E. Label-free RNA-based electrochemical nanobiosensor for detection of Hepatitis C. CURRENT RESEARCH IN BIOTECHNOLOGY 2020. [DOI: 10.1016/j.crbiot.2020.11.004] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.2] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 02/07/2023] Open
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11
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Sun H, Qiu Y, Lu Y, Kong J, Zhang X. Ultrasensitive DNA electrochemical biosensor based on MnTBAP biomimetic catalyzed AGET ATRP signal amplification reaction. Chem Commun (Camb) 2020; 56:6636-6639. [DOI: 10.1039/d0cc02176h] [Citation(s) in RCA: 7] [Impact Index Per Article: 1.4] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 12/21/2022]
Abstract
In this paper, an ultrasensitive, highly selective and green electrochemical biosensor for quantifying DNA sequences (aM DNA) based on a MnTBAP catalyst for AGET ATRP reaction is proposed.
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Affiliation(s)
- Haobo Sun
- Research Center for Biomedical and Health Science
- Anhui Science and Technology University
- Fengyang 233100
- P. R. China
- School of Environmental and Biological Engineering
| | - Yunliang Qiu
- Department of Criminal Science and Technology
- Nanjing Forest Police College
- Nanjing 210023
- P. R. China
| | - Yajie Lu
- Research Center for Biomedical and Health Science
- Anhui Science and Technology University
- Fengyang 233100
- P. R. China
| | - Jinming Kong
- School of Environmental and Biological Engineering
- Nanjing University of Science and Technology
- Nanjing 210094
- P. R. China
| | - Xueji Zhang
- School of Biomedical Engineering
- Shenzhen University Health Science Center
- Shenzhen
- P. R. China
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12
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Au and Au-Based nanomaterials: Synthesis and recent progress in electrochemical sensor applications. Talanta 2020; 206:120210. [DOI: 10.1016/j.talanta.2019.120210] [Citation(s) in RCA: 147] [Impact Index Per Article: 29.4] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 04/18/2019] [Revised: 07/25/2019] [Accepted: 07/31/2019] [Indexed: 12/28/2022]
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El-Said WA, Choi JW. High selective spectroelectrochemical biosensor for HCV-RNA detection based on a specific peptide nucleic acid. SPECTROCHIMICA ACTA. PART A, MOLECULAR AND BIOMOLECULAR SPECTROSCOPY 2019; 217:288-293. [PMID: 30952095 DOI: 10.1016/j.saa.2019.03.115] [Citation(s) in RCA: 18] [Impact Index Per Article: 3.0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 01/08/2019] [Revised: 03/28/2019] [Accepted: 03/29/2019] [Indexed: 06/09/2023]
Abstract
Hepatitis C virus (HCV) is a blood-borne virus that causes infectious chronic hepatitis. Egypt has the largest epidemic of HCV in the world, with about 14.7% of the Egyptian population. Thus, HCV, which could cause severe risks for human health including liver failure, becomes a public health concern for Egyptians. Development of highly selective and sensitive biosensors for accurate detection of HCV levels without extensive sample preparation has received great attention. The present work reported on developing a new rapid, highly selective and highly selective HCV-based biosensor for early detection of HCV-RNA extracted from clinical samples. The HCV-based biosensor was constructed by fabrication of gold nanodots/indium tin oxide substrate and followed by immobilization of a specific peptide nucleic acid (as bio-receptors) terminated with thiol group onto gold nanodots/indium tin oxide. The principle of the developed biosensor was based on the selective hybridization between the peptide nucleic acid and the HCV-RNA at the untranslated regions (5'-UTR). Raman spectroscopy and Square wave voltammetry techniques were used to monitor the interaction between the HCV-RNA and the immobilized peptide nucleic acid. The reported HCV-biosensor demonstrated a high capability to detect HCV-RNA.
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Affiliation(s)
- Waleed A El-Said
- Department of Chemistry, Faculty of Science, Assiut University, Assiut 71516, Egypt.
| | - Jeong-Woo Choi
- Department of Chemical and Biomolecular Engineering, Sogang University, 35 Baekbeom-Ro, Mapo-Gu, Seoul 04107, Republic of Korea
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Singhal C, Ingle A, Chakraborty D, PN AK, Pundir C, Narang J. Impedimetric genosensor for detection of hepatitis C virus (HCV1) DNA using viral probe on methylene blue doped silica nanoparticles. Int J Biol Macromol 2017; 98:84-93. [DOI: 10.1016/j.ijbiomac.2017.01.093] [Citation(s) in RCA: 27] [Impact Index Per Article: 3.4] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 11/18/2016] [Revised: 01/16/2017] [Accepted: 01/20/2017] [Indexed: 01/11/2023]
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15
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Zhou J, He Y, Zhang B, Sun Q, Zou G. Spectrum-based and color-selective electrochemiluminescence immunoassay for determining human prostate specific antigen in near-infrared region. Talanta 2017; 165:117-121. [DOI: 10.1016/j.talanta.2016.12.045] [Citation(s) in RCA: 19] [Impact Index Per Article: 2.4] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 10/11/2016] [Revised: 12/07/2016] [Accepted: 12/20/2016] [Indexed: 12/12/2022]
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16
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Electrochemical DNA sensors based on the use of gold nanoparticles: a review on recent developments. Mikrochim Acta 2017. [DOI: 10.1007/s00604-017-2143-1] [Citation(s) in RCA: 61] [Impact Index Per Article: 7.6] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 01/24/2023]
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17
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Zhang X, Tan X, Zhang B, Miao W, Zou G. Spectrum-Based Electrochemiluminescent Immunoassay with Ternary CdZnSe Nanocrystals as Labels. Anal Chem 2016; 88:6947-53. [DOI: 10.1021/acs.analchem.6b01821] [Citation(s) in RCA: 63] [Impact Index Per Article: 7.0] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 01/09/2023]
Affiliation(s)
- Xin Zhang
- School
of Chemistry and Chemical Engineering, Shandong University, Jinan, 250100, China
| | - Xiao Tan
- School
of Chemistry and Chemical Engineering, Shandong University, Jinan, 250100, China
| | - Bin Zhang
- School
of Chemistry and Chemical Engineering, Shandong University, Jinan, 250100, China
| | - Wujian Miao
- Department
of Chemistry and Biochemistry, The University of Southern Mississippi, Hattiesburg, Mississippi 39406-0001, United States
| | - Guizheng Zou
- School
of Chemistry and Chemical Engineering, Shandong University, Jinan, 250100, China
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18
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Zhang X, Zhang B, Miao W, Zou G. Molecular-Counting-Free and Electrochemiluminescent Single-Molecule Immunoassay with Dual-Stabilizers-Capped CdSe Nanocrystals as Labels. Anal Chem 2016; 88:5482-8. [DOI: 10.1021/acs.analchem.6b00967] [Citation(s) in RCA: 62] [Impact Index Per Article: 6.9] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 12/13/2022]
Affiliation(s)
- Xin Zhang
- School
of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, China
| | - Bin Zhang
- School
of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, China
| | - Wujian Miao
- Department
of Chemistry and Biochemistry, The University of Southern Mississippi, Hattiesburg, Mississippi 39406, United States
| | - Guizheng Zou
- School
of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, China
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19
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Polymerase chain reaction-free detection of hepatitis B virus DNA using a nanostructured impedance biosensor. Biosens Bioelectron 2016; 77:603-8. [DOI: 10.1016/j.bios.2015.10.028] [Citation(s) in RCA: 53] [Impact Index Per Article: 5.9] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 08/25/2015] [Revised: 10/03/2015] [Accepted: 10/09/2015] [Indexed: 12/12/2022]
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20
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Gerasimova YV, Kolpashchikov DM. Enzyme-assisted target recycling (EATR) for nucleic acid detection. Chem Soc Rev 2015; 43:6405-38. [PMID: 24901032 DOI: 10.1039/c4cs00083h] [Citation(s) in RCA: 160] [Impact Index Per Article: 16.0] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 12/23/2022]
Abstract
Fast, reliable and sensitive methods for nucleic acid detection are of growing practical interest with respect to molecular diagnostics of cancer, infectious and genetic diseases. Currently, PCR-based and other target amplification strategies are most extensively used in practice. At the same time, such assays have limitations that can be overcome by alternative approaches. There is a recent explosion in the design of methods that amplify the signal produced by a nucleic acid target, without changing its copy number. This review aims at systematization and critical analysis of the enzyme-assisted target recycling (EATR) signal amplification technique. The approach uses nucleases to recognize and cleave the probe-target complex. Cleavage reactions produce a detectable signal. The advantages of such techniques are potentially low sensitivity to contamination and lack of the requirement of a thermal cycler. Nucleases used for EATR include sequence-dependent restriction or nicking endonucleases or sequence independent exonuclease III, lambda exonuclease, RNase H, RNase HII, AP endonuclease, duplex-specific nuclease, DNase I, or T7 exonuclease. EATR-based assays are potentially useful for point-of-care diagnostics, single nucleotide polymorphisms genotyping and microRNA analysis. Specificity, limit of detection and the potential impact of EATR strategies on molecular diagnostics are discussed.
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Affiliation(s)
- Yulia V Gerasimova
- Chemistry Department, University of Central Florida, 4000 Central Florida Blvd., Orlando, FL 32816, USA.
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21
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Ultrasensitive strategy based on PtPd nanodendrite/nano-flower-like@GO signal amplification for the detection of long non-coding RNA. Biosens Bioelectron 2015; 74:214-21. [PMID: 26143461 DOI: 10.1016/j.bios.2015.06.021] [Citation(s) in RCA: 41] [Impact Index Per Article: 4.1] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 03/24/2015] [Revised: 05/19/2015] [Accepted: 06/07/2015] [Indexed: 12/17/2022]
Abstract
Highly up-regulated in liver cancer (HULC) is a novel promising noninvasive biomarker for hepatocellular carcinoma (HCC), which is a kind of long non-coding RNAs (lncRNAs). But traditional methods limited HULC clinical detection for ownself drawbacks. Development a new HULC detection approach is urgent and necessary. Electrochemical nucleic acid sensor based on different signal amplification strategies with high sensitivity, fast, simple, and convenient, may solve this problem. Herein, we propose a novel strategy based on Pt-Pd bimetallic nanodendrites/nanoflower-like clusters on graphene oxide/Au/horseradish peroxidase (PtPd BND/BNF@GO/Au/HRP) to enhance the catalytic efficiency and sensitivity. And Au particles were simultaneously and separately capped with thionine or detection probe, which increase the binding amount of detection probe and decrease the electronic background. The results indicated that the catalytic effect was noticeably elevated and that the biosensor provides ultrasensitive detection for the lncRNA HULC. The linear calibration of the biosensor ranged from 1.00×10(-3) to 1.00×10(3) pM/mL, and the limit of detection was 0.247 fM/mL. The lncRNA biosensor based on the PtPd BND/BNF@GO/Au/HRP/Au/thionine exhibited acceptable reproducibility and clear selectivity. This strategy may provide a new alternative for clinical HCC diagnosis through the detection of HULC.
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22
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Uliana CV, Riccardi CS, Yamanaka H. Diagnostic tests for hepatitis C: Recent trends in electrochemical immunosensor and genosensor analysis. World J Gastroenterol 2014; 20:15476-15491. [PMID: 25400433 PMCID: PMC4229514 DOI: 10.3748/wjg.v20.i42.15476] [Citation(s) in RCA: 23] [Impact Index Per Article: 2.1] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Download PDF] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Received: 11/07/2013] [Revised: 02/19/2014] [Accepted: 06/13/2014] [Indexed: 02/06/2023] Open
Abstract
Hepatitis C is a liver disease that is transmitted through contact with the blood of an infected person. An estimated 150 million individuals worldwide have been chronically infected with the hepatitis C virus (HCV). Hepatitis C shows significant genetic variation in the global population, due to the high rate of viral RNA mutation. There are six variants of the virus (HCV genotypes 1, 2, 3, 4, 5, and 6), with 15 recorded subtypes that vary in prevalence across different regions of the world. A variety of devices are used to diagnose hepatitis C, including HCV antibody test, HCV viral load test, HCV genotype test and liver biopsy. Rapid, inexpensive, sensitive, and robust analytical devices are therefore essential for effective diagnosis and monitoring of disease treatment. This review provides an overview of current electrochemical immunosensor and genosensor technologies employed in HCV detection. There are a limited number of publications showing electrochemical biosensors being used for the detection of HCV. Due to their simplicity, specificity, and reliability, electrochemical biosensor devices have potential clinical applications in several viral infections.
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23
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Lee TY, Shin Y, Park MK. A simple, low-cost, and rapid device for a DNA methylation-specific amplification/detection system using a flexible plastic and silicon complex. LAB ON A CHIP 2014; 14:4220-9. [PMID: 25184832 DOI: 10.1039/c4lc00804a] [Citation(s) in RCA: 21] [Impact Index Per Article: 1.9] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 05/10/2023]
Abstract
Abnormal DNA methylation has been associated with the development and progression of several human cancers and is a potential target for treatment. Thus, myriad technologies for the analysis of DNA methylation have been developed over the past few decades. However, most of these technologies are still far from ideal because they are time-consuming, labor-intensive, and complex, and there is the risk of contamination of samples. Here, we present an innovative DNA methylation-specific amplification/detection device for analysis of DNA methylation in cancer-related DNA biomarkers. The assay is based on a microfluidic system that is coupled to a flexible plastic-based on-chip endonuclease digestion device with optimized magnetic field effect and a methylation-specific isothermal solid-phase amplification/detection technique to allow a low-cost, simple, and rapid analysis of DNA methylation status in a label-free and real-time manner. This flexible plastic/silicon-based microfluidic device is relatively simple to fabricate with a flexible thin film and a magnet array by using a laser machine that can overcome the limitations of a PDMS-based microfluidic device. We demonstrated the ability of the methylation analysis based on the proposed flexible device to detect the methylated RARβ gene, which is a common DNA methylation biomarker in several human cancers. The simple platform detected the methylated gene in genomic DNA from human cancer cell lines within 65 min, whereas other methods required at least several hours. Therefore, this simple, low-cost, and rapid methylation analysis platform will be useful for the detection of DNA methylation in point-of-care applications.
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Affiliation(s)
- Tae Yoon Lee
- Institute of Microelectronics, A*STAR (Agency for Science, Technology and Research), 11 Science Park Road, Singapore Science Park II, Singapore 117685.
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24
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Hu Q, Hu W, Kong J, Zhang X. PNA-based DNA assay with attomolar detection limit based on polygalacturonic acid mediated in-situ deposition of metallic silver on a gold electrode. Mikrochim Acta 2014. [DOI: 10.1007/s00604-014-1351-1] [Citation(s) in RCA: 14] [Impact Index Per Article: 1.3] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 12/18/2022]
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25
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Tian R, Qu Y, Zheng X. Amplified fluorescence quenching of lucigenin self-assembled inside silica/chitosan nanoparticles by Cl⁻. Anal Chem 2014; 86:9114-21. [PMID: 25135186 DOI: 10.1021/ac5018502] [Citation(s) in RCA: 21] [Impact Index Per Article: 1.9] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 01/20/2023]
Abstract
Fluorescence sensing of an analyte based on the fluorophore collective effect is a reliable, sensitive sensing approach. Many ultralow targets can be detected on the basis of the high sensitivity and signal amplification of the fluorescence sensing system. However, the complicated synthesis procedures, harsh conditions required to design and control the fluorescence molecular probes and conjugated chain length, and the higher cost of synthesis are still challenges. To address these issues, we developed a simple, rapid, and sensitive collective effect based fluorescence sensing platform. In this sensing platform, the fluorophore unit was self-assembled on the wall of the nanopores of the porous structural silica/chitosan nanoparticles (SCNPs) on the basis of the electrostatic interaction and supermolecular interaction between the fluorophores and SiO(-) groups and chitosan. Since these self-assembled fluorophores are close enough to communicate with each other on the basis of the space confinement effect of the pore size, many fluorophore units could interact with a single analyte and produce an amplified fluorescence sensing ability. Chloride ion, an important anion in biological fluids, and lucigenin, a typical fluorescent dye, were used as a model to confirm the proof-of-concept strategy. Our results showed that, compared to free-state lucigenin in solution, the assembled-state lucigenin in SCNPs presented an about 10-fold increase in its Stern-Volmer constant when the concentration of Cl(-) was lower than 10 mM, and this fluorescence nanosensor was also successfully used to sense the chloride ion in living cells.
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Affiliation(s)
- Rui Tian
- Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry & Chemical Engineering, Shaanxi Normal University , Xi'an, 710062 Shaanxi, People's Republic of China
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26
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Liu L, Xing Y, Zhang H, Liu R, Liu H, Xia N. Amplified voltammetric detection of glycoproteins using 4-mercaptophenylboronic acid/biotin-modified multifunctional gold nanoparticles as labels. Int J Nanomedicine 2014; 9:2619-26. [PMID: 24920899 PMCID: PMC4043723 DOI: 10.2147/ijn.s62343] [Citation(s) in RCA: 4] [Impact Index Per Article: 0.4] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 01/25/2023] Open
Abstract
Ultrasensitive detection of protein biomarkers is essential for early diagnosis and therapy of many diseases. Glycoproteins, differing from other types of proteins, contain carbohydrate moieties in the oligosaccharide chains. Boronic acid can form boronate ester covalent bonds with diol-containing species. Herein, we present a sensitive and cost-effective electrochemical method for glycoprotein detection using 4-mercaptophenylboronic acid (MBA)/biotin-modified gold nanoparticles (AuNPs) (MBA-biotin-AuNPs) as labels. To demonstrate the feasibility and sensitivity of this method, recombinant human erythropoietin (rHuEPO) was tested as a model analyte. Specifically, rHuEPO was captured by the anti-rHuEPO aptamer-covered electrode and then derivatized with MBA-biotin-AuNPs through the boronic acid-carbohydrate interaction. The MBA-biotin-AuNPs facilitated the attachment of streptavidin-conjugated alkaline phosphatase for the production of electroactive p-aminophenol from p-aminophenyl phosphate substrate. A detection limit of 8 fmol L(-1) for rHuEPO detection was achieved. Other glycosylated and non-glycosylated proteins, such as horseradish peroxidase, prostate specific antigen, metallothionein, streptavidin, and thrombin showed no interference in the detection assay.
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Affiliation(s)
- Lin Liu
- College of Chemistry and Chemical Engineering, Anyang Normal University, Anyang, Henan, People's Republic of China ; College of Chemistry and Chemical Engineering, Central South University, Changsha, Hunan, People's Republic of China
| | - Yun Xing
- College of Chemistry and Chemical Engineering, Anyang Normal University, Anyang, Henan, People's Republic of China
| | - Hui Zhang
- College of Chemistry and Chemical Engineering, Anyang Normal University, Anyang, Henan, People's Republic of China
| | - Ruili Liu
- College of Chemistry and Chemical Engineering, Anyang Normal University, Anyang, Henan, People's Republic of China
| | - Huijing Liu
- College of Chemistry and Chemical Engineering, Anyang Normal University, Anyang, Henan, People's Republic of China
| | - Ning Xia
- College of Chemistry and Chemical Engineering, Anyang Normal University, Anyang, Henan, People's Republic of China ; College of Chemistry and Chemical Engineering, Central South University, Changsha, Hunan, People's Republic of China
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27
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Liu S, Zhang X, Yu Y, Zou G. A Monochromatic Electrochemiluminescence Sensing Strategy for Dopamine with Dual-Stabilizers-Capped CdSe Quantum Dots as Emitters. Anal Chem 2014; 86:2784-8. [DOI: 10.1021/ac500046s] [Citation(s) in RCA: 107] [Impact Index Per Article: 9.7] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 02/08/2023]
Affiliation(s)
- Shufeng Liu
- School
of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, China
| | - Xin Zhang
- School
of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, China
| | - Yanmin Yu
- Department
of Chemistry and Chemical Engineering, College of Environmental and
Energy Engineering, Beijing University of Technology, Beijing 100124, China
| | - Guizheng Zou
- School
of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, China
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28
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Mehdi Khoshfetrat S, Mehrgardi MA. Dual amplification of single nucleotide polymorphism detection using graphene oxide and nanoporous gold electrode platform. Analyst 2014; 139:5192-9. [DOI: 10.1039/c4an01171f] [Citation(s) in RCA: 17] [Impact Index Per Article: 1.5] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 01/14/2023]
Abstract
In the present manuscript, a strategy to prompt the sensitivity of a biosensor based on the dual amplification of signal by applying a nanoporous gold electrode (NPGE) as a support platform and soluble graphene oxide (GO) as an indicator has been developed.
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29
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Hu R, Wen W, Wang Q, Xiong H, Zhang X, Gu H, Wang S. Novel electrochemical aptamer biosensor based on an enzyme-gold nanoparticle dual label for the ultrasensitive detection of epithelial tumour marker MUC1. Biosens Bioelectron 2013; 53:384-9. [PMID: 24189297 DOI: 10.1016/j.bios.2013.10.015] [Citation(s) in RCA: 102] [Impact Index Per Article: 8.5] [Reference Citation Analysis] [Abstract] [Key Words] [Journal Information] [Subscribe] [Scholar Register] [Received: 08/28/2013] [Revised: 10/10/2013] [Accepted: 10/10/2013] [Indexed: 01/27/2023]
Abstract
A novel platform based on a hairpin oligonucleotide (HO) switch, gold nanoparticles (AuNPs), and enzyme signal amplification for the ultrasensitive detection of mucin 1 protein (MUC1) was developed in this assay. This HO aptamers and horseradish peroxidase (HRP) were immobilised on the AuNPs to yield HO-AuNP-HRP conjugates. AuNPs were used as labels and bridges between the HO and HRP. HRP was also used as label for catalysing the oxidation of o-phenylenediamine by H2O2. The reaction product was 2,3-diaminophenazine (DAP), which was reduced and could be detected at surface of modified electrode. The reduction signal of DAP was used as a probe for the sensitive detection. After the recognition between oligonucleotide and MUC1, biotin was exposed. Biotin, along with the conjugate, was captured by streptavidin onto the surface of modified electrode. Therefore, the detection of target MUC1 which was a membrane-associated glycoprotein of the mucin family could be sensitively transduced via detection of the electrochemical reduction signal of DAP. Compared to other aptasensors, this biosensor has a good linear correlation ranges from 8.8 nM to 353.3 nM and a lower detection limit of 2.2 nM for MUC1. The proposed method provided a new electrochemical approach for the detection of MUC1.
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Affiliation(s)
- Rong Hu
- Hubei Collaborative Innovation Center for Advanced Organic Chemical Materials, Ministry of Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules & College of Chemistry and Chemical Engineering, Hubei University, Wuhan 430062, PR China
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30
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Signal-on impedimetric electrochemical DNA sensor using dithiothreitol modified gold nanoparticle tag for highly sensitive DNA detection. Anal Chim Acta 2013; 799:36-43. [DOI: 10.1016/j.aca.2013.09.024] [Citation(s) in RCA: 18] [Impact Index Per Article: 1.5] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 06/07/2013] [Revised: 09/03/2013] [Accepted: 09/09/2013] [Indexed: 11/15/2022]
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31
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Lereau M, Fournier-Wirth C, Mayen J, Farre C, Meyer A, Dugas V, Cantaloube JF, Chaix C, Vasseur JJ, Morvan F. Development of Innovative and Versatile Polythiol Probes for Use on ELOSA or Electrochemical Biosensors: Application in Hepatitis C Virus Genotyping. Anal Chem 2013; 85:9204-12. [DOI: 10.1021/ac401941x] [Citation(s) in RCA: 16] [Impact Index Per Article: 1.3] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 12/13/2022]
Affiliation(s)
- Myriam Lereau
- Laboratoire
TransDiag—Sécurité Transfusionnelle et Innovation
Diagnostique, Etablissement Français du Sang Pyrénées-Méditerranée, Montpellier, 34184, France
- Département
des Analogues et Constituants des Acides Nucléiques, Institut
des Biomolécules Max Mousseron, UMR 5247 CNRS Université Montpellier 1 Université Montpellier 2, Montpellier, 34095, France
| | - Chantal Fournier-Wirth
- Laboratoire
TransDiag—Sécurité Transfusionnelle et Innovation
Diagnostique, Etablissement Français du Sang Pyrénées-Méditerranée, Montpellier, 34184, France
| | - Julie Mayen
- Département
des Analogues et Constituants des Acides Nucléiques, Institut
des Biomolécules Max Mousseron, UMR 5247 CNRS Université Montpellier 1 Université Montpellier 2, Montpellier, 34095, France
| | - Carole Farre
- Département
Laboratoire des Sciences Analytiques, Institut des Sciences Analytiques, UMR 5280 CNRS Université de Lyon, Université Lyon 1, Villeurbanne, 69100, France
| | - Albert Meyer
- Département
des Analogues et Constituants des Acides Nucléiques, Institut
des Biomolécules Max Mousseron, UMR 5247 CNRS Université Montpellier 1 Université Montpellier 2, Montpellier, 34095, France
| | - Vincent Dugas
- Département
Laboratoire des Sciences Analytiques, Institut des Sciences Analytiques, UMR 5280 CNRS Université de Lyon, Université Lyon 1, Villeurbanne, 69100, France
| | - Jean-François Cantaloube
- Laboratoire
Emergence et Co-évolution Virale, Etablissement Français du Sang Alpes-Méditerranée, Marseille, 13005, France
| | - Carole Chaix
- Département
Laboratoire des Sciences Analytiques, Institut des Sciences Analytiques, UMR 5280 CNRS Université de Lyon, Université Lyon 1, Villeurbanne, 69100, France
| | - Jean-Jacques Vasseur
- Département
des Analogues et Constituants des Acides Nucléiques, Institut
des Biomolécules Max Mousseron, UMR 5247 CNRS Université Montpellier 1 Université Montpellier 2, Montpellier, 34095, France
| | - François Morvan
- Département
des Analogues et Constituants des Acides Nucléiques, Institut
des Biomolécules Max Mousseron, UMR 5247 CNRS Université Montpellier 1 Université Montpellier 2, Montpellier, 34095, France
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32
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Highly sensitive detection of DNA using an electrochemical DNA sensor with thionine-capped DNA/gold nanoparticle conjugates as signal tags. Electrochem commun 2013. [DOI: 10.1016/j.elecom.2013.05.015] [Citation(s) in RCA: 25] [Impact Index Per Article: 2.1] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/21/2022] Open
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33
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Zhang AH, Sun H, Han Y, Yan GL, Yuan Y, Song GC, Yuan XX, Xie N, Wang XJ. Ultraperformance liquid chromatography-mass spectrometry based comprehensive metabolomics combined with pattern recognition and network analysis methods for characterization of metabolites and metabolic pathways from biological data sets. Anal Chem 2013; 85:7606-12. [PMID: 23845028 DOI: 10.1021/ac401793d] [Citation(s) in RCA: 76] [Impact Index Per Article: 6.3] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 02/06/2023]
Abstract
Metabolomics is the study of metabolic changes in biological systems and provides the small molecule fingerprints related to the disease. Extracting biomedical information from large metabolomics data sets by multivariate data analysis is of considerable complexity. Therefore, more efficient and optimizing metabolomics data processing technologies are needed to improve mass spectrometry applications in biomarker discovery. Here, we report the findings of urine metabolomic investigation of hepatitis C virus (HCV) patients by high-throughput ultraperformance liquid chromatography-mass spectrometry (UPLC-MS) coupled with pattern recognition methods (principal component analysis, partial least-squares, and OPLS-DA) and network pharmacology. A total of 20 urinary differential metabolites (13 upregulated and 7 downregulated) were identified and contributed to HCV progress, involve several key metabolic pathways such as taurine and hypotaurine metabolism, glycine, serine and threonine metabolism, histidine metabolism, arginine and proline metabolism, and so forth. Metabolites identified through metabolic profiling may facilitate the development of more accurate marker algorithms to better monitor disease progression. Network analysis validated close contact between these metabolites and implied the importance of the metabolic pathways. Mapping altered metabolites to KEGG pathways identified alterations in a variety of biological processes mediated through complex networks. These findings may be promising to yield a valuable and noninvasive tool that insights into the pathophysiology of HCV and to advance the early diagnosis and monitor the progression of disease. Overall, this investigation illustrates the power of the UPLC-MS platform combined with the pattern recognition and network analysis methods that can engender new insights into HCV pathobiology.
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Affiliation(s)
- Ai-hua Zhang
- National TCM Key Laboratory of Serum Pharmacochemistry, Key Laboratory of Chinmedomics, Heilongjiang University of Chinese Medicine, Harbin 150040, China
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34
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Su S, Zuo X, Pan D, Pei H, Wang L, Fan C, Huang W. Design and applications of gold nanoparticle conjugates by exploiting biomolecule-gold nanoparticle interactions. NANOSCALE 2013; 5:2589-2599. [PMID: 23423633 DOI: 10.1039/c3nr33870c] [Citation(s) in RCA: 50] [Impact Index Per Article: 4.2] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 06/01/2023]
Abstract
Gold nanoparticles (AuNPs) are a type of widely used nanomaterials with unique chemical and physical properties. AuNPs can be readily synthesized, and modified with various chemical or biological molecules, making them promising candidates for catalysis, drug delivery and biological imaging applications. In this review, we mainly focus on recent advances in the design and synthesis of conjugates of AuNPs by exploiting biomolecule-AuNP interactions. We will also discuss a variety of bioapplications of AuNP-based conjugates.
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Affiliation(s)
- Shao Su
- Key Laboratory for Organic Electronics & Information Displays, Institute of Advanced Materials, School of Materials Science and Engineering, Nanjing University of Posts & Telecommunications, 9 Wenyuan Road, Nanjing, 210046, China
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35
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Cheng MS, Toh CS. Novel biosensing methodologies for ultrasensitive detection of viruses. Analyst 2013; 138:6219-29. [DOI: 10.1039/c3an01394d] [Citation(s) in RCA: 36] [Impact Index Per Article: 3.0] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 01/07/2023]
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36
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Shao X, Gu H, Wang Z, Chai X, Tian Y, Shi G. Highly Selective Electrochemical Strategy for Monitoring of Cerebral Cu2+ Based on a Carbon Dot-TPEA Hybridized Surface. Anal Chem 2012; 85:418-25. [DOI: 10.1021/ac303113n] [Citation(s) in RCA: 141] [Impact Index Per Article: 10.8] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 02/08/2023]
Affiliation(s)
- Xiangling Shao
- Department
of Chemistry, Tongji University, Siping Road 1239, Shanghai 200092, People’s Republic of
China, and
| | - Hui Gu
- Department
of Chemistry, East China Normal University, Dongchuan Road 500, Shanghai 200241, People’s Republic of
China
| | - Zhen Wang
- Department
of Chemistry, Tongji University, Siping Road 1239, Shanghai 200092, People’s Republic of
China, and
| | - Xiaolan Chai
- Department
of Chemistry, Tongji University, Siping Road 1239, Shanghai 200092, People’s Republic of
China, and
| | - Yang Tian
- Department
of Chemistry, Tongji University, Siping Road 1239, Shanghai 200092, People’s Republic of
China, and
| | - Guoyue Shi
- Department
of Chemistry, East China Normal University, Dongchuan Road 500, Shanghai 200241, People’s Republic of
China
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37
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Tang J, Hou L, Tang D, Zhou J, Wang Z, Li J, Chen G. Magneto-controlled electrochemical immunoassay of brevetoxin B in seafood based on guanine-functionalized graphene nanoribbons. Biosens Bioelectron 2012; 38:86-93. [DOI: 10.1016/j.bios.2012.05.006] [Citation(s) in RCA: 29] [Impact Index Per Article: 2.2] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 03/20/2012] [Revised: 05/04/2012] [Accepted: 05/07/2012] [Indexed: 02/02/2023]
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38
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Li W, Wu P, Zhang H, Cai C. Signal Amplification of Graphene Oxide Combining with Restriction Endonuclease for Site-Specific Determination of DNA Methylation and Assay of Methyltransferase Activity. Anal Chem 2012; 84:7583-90. [DOI: 10.1021/ac301990f] [Citation(s) in RCA: 134] [Impact Index Per Article: 10.3] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 12/15/2022]
Affiliation(s)
- Wen Li
- Jiangsu Key Laboratory of New Power Batteries, College
of Chemistry and Materials Science, Nanjing Normal University, Nanjing 210097, P. R. China
| | - Ping Wu
- Jiangsu Key Laboratory of New Power Batteries, College
of Chemistry and Materials Science, Nanjing Normal University, Nanjing 210097, P. R. China
| | - Hui Zhang
- Jiangsu Key Laboratory of New Power Batteries, College
of Chemistry and Materials Science, Nanjing Normal University, Nanjing 210097, P. R. China
| | - Chenxin Cai
- Jiangsu Key Laboratory of New Power Batteries, College
of Chemistry and Materials Science, Nanjing Normal University, Nanjing 210097, P. R. China
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Endonuclease cleavage combined with horseradish peroxidase-assisted signal amplification for electrochemical monitoring of DNA. Electrochem commun 2012. [DOI: 10.1016/j.elecom.2012.06.017] [Citation(s) in RCA: 6] [Impact Index Per Article: 0.5] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 02/04/2023] Open
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Liu M, Zhao H, Chen S, Yu H, Quan X. Interface engineering catalytic graphene for smart colorimetric biosensing. ACS NANO 2012; 6:3142-51. [PMID: 22443302 DOI: 10.1021/nn3010922] [Citation(s) in RCA: 199] [Impact Index Per Article: 15.3] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 05/18/2023]
Abstract
Herein a hybrid catalyst consisting of "naked" Au-NPs in situ grown on graphene sheets is engineered, which exhibits a synergetic effect in mimicking peroxidase at its interface, although free Au-NPs or graphene alone has very little activity. What is more, one of the unique features of our synergetic catalyst is that its interface can be reversibly switched from "inactive" to "active" upon treatment with different ssDNA species in solution, thus providing a powerful and versatile basis for designing graphene/DNA-based label-free colorimetric biosensors. Compared with other signal transduction modes in traditional graphene/aptamer-based systems, our novel signaling strategy not only avoids any labeling or modification procedures but also reduces the background signal due to the "off-on" switching mode during the sensing. Furthermore, this facile and general approach can be applicable to the other extended graphene/aptamer-based systems for colorimetric detection of a wide range of analytes. We envision that the tunable graphene-based smart interface could find potential applications in the development of biocatalysis, bioassays, and smart material devices in the future.
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Affiliation(s)
- Meng Liu
- School of Environmental Science and Technology, Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education, China), Dalian University of Technology, Dalian, 116024, China
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Li W, Wu P, Zhang H, Cai C. Catalytic signal amplification of gold nanoparticles combining with conformation-switched hairpin DNA probe for hepatitis C virus quantification. Chem Commun (Camb) 2012; 48:7877-9. [DOI: 10.1039/c2cc33635a] [Citation(s) in RCA: 24] [Impact Index Per Article: 1.8] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 02/05/2023]
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Tang D, Tang J, Su B, Li Q, Chen G. Electrochemical detection of hepatitis C virus with signal amplification using BamHI endonuclease and horseradish peroxidase-encapsulated nanogold hollow spheres. Chem Commun (Camb) 2011; 47:9477-9. [PMID: 21785766 DOI: 10.1039/c1cc13340c] [Citation(s) in RCA: 22] [Impact Index Per Article: 1.6] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 12/20/2022]
Abstract
A novel electrochemical method to detect hepatitis C virus was developed based on site-specific cleavage of BamHI endonuclease and enzymatic signal amplification with horseradish peroxidase-encapsulated nanogold hollow spheres.
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Affiliation(s)
- Dianping Tang
- Key Laboratory of Analysis and Detection of Food Safety (Ministry of Education & Fujian Province), Department of Chemistry, Fuzhou University, Fuzhou 350108, PR China.
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