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Xie X, Cai X, Zhou F, Li Y, Liu Q, Cai L, Zhu W, Wei J, Jin C, Liu Z, Jiang C, Zhao H, Yang L, Zhao C, Huang X. GPR37 promotes cancer growth by binding to CDK6 and represents a new theranostic target in lung adenocarcinoma. Pharmacol Res 2022; 183:106389. [PMID: 35934193 DOI: 10.1016/j.phrs.2022.106389] [Citation(s) in RCA: 22] [Impact Index Per Article: 7.3] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Received: 04/15/2022] [Revised: 08/02/2022] [Accepted: 08/03/2022] [Indexed: 01/11/2023]
Abstract
Lung adenocarcinoma (LUAD) is associated with poor prognosis. Identifying novel cancer targets and helpful therapeutic strategies remains a serious clinical challenge. This study detected differentially expressed genes in The Cancer Genome Atlas (TCGA) LUAD data collection. We also identified a predictive DNA biomarker, G protein-coupled receptor 37 (GPR37), which was verified as a prognostic biomarker with a critical role in tumor progression. In human LUAD specimens and microarray analyses, we determined that GPR37 was significantly upregulated and associated with a poor prognosis. GPR37 downregulation markedly inhibited the proliferation and migration of LUAD both in vitro and in vivo. Mechanistically, GPR37 could bind to CDK6, thereby facilitating tumor progression in LUAD by inducing cell cycle arrest at the G1 phase. GPR37 also facilitates tumorigenesis in xenograft tumors in vivo. High-throughput screening for GPR37-targeted drugs was performed using the Natural Products Library, which revealed the potential of Hypocrellin B to inhibit GPR37 and cell growth in LUAD. We demonstrated that Hypocrellin B suppressed LUAD cell proliferation and migration both in vitro and in vivo via GPR37 inhibition. Collectively, our findings reveal the role of GPR37 in LUAD progression and migration and the potential of GPR37 as a target for the treatment of LUAD. Thus, the specific inhibition of GPR37 by the natural product Hypocrellin B may possess the potential for the treatment of LUAD.
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Affiliation(s)
- Xiaona Xie
- Division of Pulmonary Medicine, The First Affiliated Hospital of Wenzhou Medical University, Key Laboratory of Heart and Lung, Wenzhou, Zhejiang 325000, China; Department of Medical Oncology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, China
| | - Xueding Cai
- Division of Pulmonary Medicine, The First Affiliated Hospital of Wenzhou Medical University, Key Laboratory of Heart and Lung, Wenzhou, Zhejiang 325000, China
| | - Feng Zhou
- Division of Pulmonary Medicine, The First Affiliated Hospital of Wenzhou Medical University, Key Laboratory of Heart and Lung, Wenzhou, Zhejiang 325000, China
| | - Yaozhe Li
- Division of Pulmonary Medicine, The First Affiliated Hospital of Wenzhou Medical University, Key Laboratory of Heart and Lung, Wenzhou, Zhejiang 325000, China
| | - Qianzi Liu
- The Institute of Life Sciences, Wenzhou University, University Town, Wenzhou, Zhejiang 325035, China
| | - Luqiong Cai
- Division of Pulmonary Medicine, The First Affiliated Hospital of Wenzhou Medical University, Key Laboratory of Heart and Lung, Wenzhou, Zhejiang 325000, China
| | - Wenjing Zhu
- Division of Pulmonary Medicine, The First Affiliated Hospital of Wenzhou Medical University, Key Laboratory of Heart and Lung, Wenzhou, Zhejiang 325000, China
| | - Jinqiu Wei
- Division of Pulmonary Medicine, The First Affiliated Hospital of Wenzhou Medical University, Key Laboratory of Heart and Lung, Wenzhou, Zhejiang 325000, China
| | - Chenying Jin
- School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou 325035, Zhejiang, China
| | - Zitian Liu
- Department of Thoracic Surgery, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, China
| | - Chunhui Jiang
- School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou 325035, Zhejiang, China
| | - Haiyang Zhao
- The Institute of Life Sciences, Wenzhou University, University Town, Wenzhou, Zhejiang 325035, China
| | - Lehe Yang
- Division of Pulmonary Medicine, The First Affiliated Hospital of Wenzhou Medical University, Key Laboratory of Heart and Lung, Wenzhou, Zhejiang 325000, China.
| | - Chengguang Zhao
- Division of Pulmonary Medicine, The First Affiliated Hospital of Wenzhou Medical University, Key Laboratory of Heart and Lung, Wenzhou, Zhejiang 325000, China; School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou 325035, Zhejiang, China.
| | - Xiaoying Huang
- Division of Pulmonary Medicine, The First Affiliated Hospital of Wenzhou Medical University, Key Laboratory of Heart and Lung, Wenzhou, Zhejiang 325000, China.
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Photodynamic inactivation of Staphylococcus aureus in the system of titanium dioxide nanoparticles sensitized by hypocrellin B and its application in food preservation. Food Res Int 2022; 156:111141. [DOI: 10.1016/j.foodres.2022.111141] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 11/26/2021] [Revised: 03/11/2022] [Accepted: 03/13/2022] [Indexed: 11/20/2022]
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Yan J, Gao T, Lu Z, Yin J, Zhang Y, Pei R. Aptamer-Targeted Photodynamic Platforms for Tumor Therapy. ACS APPLIED MATERIALS & INTERFACES 2021; 13:27749-27773. [PMID: 34110790 DOI: 10.1021/acsami.1c06818] [Citation(s) in RCA: 49] [Impact Index Per Article: 12.3] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 06/12/2023]
Abstract
Achieving controlled and accurate delivery of photosensitizers (PSs) into tumor sites is a major challenge in conventional photodynamic therapy (PDT). Aptamer is a short oligonucleotide sequence (DNA or RNA) with a folded three-dimensional structure, which can selectively bind to specific small molecules, proteins, or the whole cells. Aptamers could act as ligands and be modified onto PSs or nanocarriers, enabling specific recognition and binding to tumor cells or their membrane proteins. The resultant aptamer-modified PSs or PSs-containing nanocarriers generate amounts of reactive oxygen species with light irradiation and obtain superior photodynamic therapeutic efficiency in tumors. Herein, we overview the recent progress in the designs and applications of aptamer-targeted photodynamic platforms for tumor therapy. First, we focus on the progress on the rational selection of aptamers and summarize the applications of aptamers which have been applied for targeted tumor diagnosis and therapy. Then, aptamer-targeted photodynamic therapies including various aptamer-PSs, aptamer-nanocarriers containing PSs, and aptamer-nano-photosensitizers are highlighted. The aptamer-targeted synergistically therapeutic platforms including PDT, photothermal therapy, and chemotherapy, as well as the imaging-guided theranostics, are also discussed. Finally, we offer an insight into the development trends and future perspectives of aptamer-targeted photodynamic platforms for tumor therapy.
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Affiliation(s)
- Jincong Yan
- Department of Polymer Materials, School of Materials Science and Engineering, Shanghai University, 200444 Shanghai, China
- CAS Key Laboratory for Nano-Bio Interface, Suzhou Institute of Nano-tech and Nano-bionics, Chinese Academy of Sciences, 215123 Suzhou, China
| | - Tian Gao
- CAS Key Laboratory for Nano-Bio Interface, Suzhou Institute of Nano-tech and Nano-bionics, Chinese Academy of Sciences, 215123 Suzhou, China
| | - Zhongzhong Lu
- CAS Key Laboratory for Nano-Bio Interface, Suzhou Institute of Nano-tech and Nano-bionics, Chinese Academy of Sciences, 215123 Suzhou, China
| | - Jingbo Yin
- Department of Polymer Materials, School of Materials Science and Engineering, Shanghai University, 200444 Shanghai, China
| | - Ye Zhang
- CAS Key Laboratory for Nano-Bio Interface, Suzhou Institute of Nano-tech and Nano-bionics, Chinese Academy of Sciences, 215123 Suzhou, China
| | - Renjun Pei
- CAS Key Laboratory for Nano-Bio Interface, Suzhou Institute of Nano-tech and Nano-bionics, Chinese Academy of Sciences, 215123 Suzhou, China
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Li Y, Xu Y, Liao Q, Xie M, Tao H, Wang HL. Synergistic effect of hypocrellin B and curcumin on photodynamic inactivation of Staphylococcus aureus. Microb Biotechnol 2021; 14:692-707. [PMID: 33465291 PMCID: PMC7936292 DOI: 10.1111/1751-7915.13734] [Citation(s) in RCA: 14] [Impact Index Per Article: 3.5] [Reference Citation Analysis] [Abstract] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 08/13/2020] [Revised: 10/22/2020] [Accepted: 12/06/2020] [Indexed: 01/09/2023] Open
Abstract
Antimicrobial photodynamic inactivation (aPDI) serves as a new approach to control the growth of foodborne bacteria. It remains elusive if the photodynamic efficacy of hypocrellin B (HB) can be potentiated by joint action with curcumin. In this study, we measured the survival rate of Staphylococcus aureus strains under the varying photodynamic conditions. According to our data, a maximum of 5–6 log10 decrease of bacterial survival can be achieved under the tested conditions (500 nM, 9 J cm‒2). Regarding the bactericidal mechanisms, HB‐based aPDI disrupted the membrane integrity of staphylococcal cells, probably owing to the stimulated reactive oxygen species (ROS). In addition, aPDI disrupted the enzymatic activities of bacterial antioxidant proteins and caused the leakage of multiple intracellular substances. The HB‐mediated photodynamic efficacy was potentiated by the addition of curcumin with a sublethal dose. This dual‐photon synergy arose from unique aPDI conditions (100 nM each and 9 J cm‒2). The synergistic action might be accounted for by the increased type I/type II ratio of ROS, as evidenced by the effect of different quenchers. Finally, the joint use of photosensitizers reduced the microbial contamination of the tested apple while maintaining its quality. In summary, photodynamic inactivation based on dual photons showed synergistic activity in controlling the growth of Staphylococcal aureus, which provided a novel approach to maintain food safety.
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Affiliation(s)
- Yali Li
- School of Food and Bioengineering, Hefei University of Technology, Hefei, 230009, China
| | - Yi Xu
- School of Food and Bioengineering, Hefei University of Technology, Hefei, 230009, China
| | - Qiaoming Liao
- School of Food and Bioengineering, Hefei University of Technology, Hefei, 230009, China
| | - Mengmeng Xie
- School of Food and Bioengineering, Hefei University of Technology, Hefei, 230009, China
| | - Han Tao
- School of Food and Bioengineering, Hefei University of Technology, Hefei, 230009, China
| | - Hui-Li Wang
- School of Food and Bioengineering, Hefei University of Technology, Hefei, 230009, China.,Engineering Research Center of Bio-process, Ministry of Education, Hefei University of Technology, 193 Tunxi Road, Hefei, 230009, China
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Ding N, Chang X, Shi N, Yin X, Qi F, Sun Y. Enhanced inactivation of antibiotic-resistant bacteria isolated from secondary effluents by g-C 3N 4 photocatalysis. ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH INTERNATIONAL 2019; 26:18730-18738. [PMID: 31055749 DOI: 10.1007/s11356-019-05080-7] [Citation(s) in RCA: 11] [Impact Index Per Article: 1.8] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 01/09/2019] [Accepted: 04/02/2019] [Indexed: 06/09/2023]
Abstract
The extensive use of antibiotics has resulted in the development of antibiotic-resistant bacteria (ARB), which may not be completely removed by traditional wastewater treatment processes. More effective approaches to disinfection are needed to prevent the release of ARB into the surface water. The metal-free photocatalyst graphitic carbon nitride (g-C3N4) has aroused great interest as a possible agent for water and wastewater treatment, due to its low cytotoxicity and photoactivity with visible light. In this study, the efficacy of g-C3N4 was assessed as a possible means to enhance ARB inactivation by irradiation. ARB were isolated and purified from secondary effluents in 4 municipal wastewater treatment plants. Of these, 4 typical multi-drug ARB isolates, belonging to Enterobacteriaceae, were selected for irradiation experiments. Inactivation was seen to increase with irradiation time. At 60 min, the inactivation of the 4 ARB isolates by light at > 300 nm and > 400 nm was in the range of 0.25-0.39 log and 0.16-0.19 log, respectively. The use of g-C3N4-mediated photocatalysis at the same wavelengths significantly enhanced that to 0.64-1.26 log and 0.31-0.41 log, respectively. The antibiotic susceptibility of the ARB isolates remained unchanged either prior to or after irradiation and was independent of photon fluence, reaction time, and the presence of g-C3N4. This study establishes a baseline for understanding the effectiveness of g-C3N4 photocatalysis on inactivation of ARB in wastewaters and lays the foundation for further improvement in the use of photocatalysis for wastewater treatment.
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Affiliation(s)
- Ning Ding
- Department of Environmental Science and Engineering, Beijing Technology and Business University, Fucheng Road No.11, Haidian District, Beijing, 100048, China
- Key Laboratory of Cleaner Production and Comprehensive Utilization of Resources, China National Light Industry, Beijing Technology and Business University, Beijing, China
| | - Xueming Chang
- Department of Environmental Science and Engineering, Beijing Technology and Business University, Fucheng Road No.11, Haidian District, Beijing, 100048, China
| | - Na Shi
- Beijing Boda Water Co., Ltd, Beijing, China
| | - Xiufeng Yin
- Department of Environmental Science and Engineering, Beijing Technology and Business University, Fucheng Road No.11, Haidian District, Beijing, 100048, China
| | - Fei Qi
- Department of Environmental Science and Engineering, Beijing Technology and Business University, Fucheng Road No.11, Haidian District, Beijing, 100048, China
| | - Yingxue Sun
- Department of Environmental Science and Engineering, Beijing Technology and Business University, Fucheng Road No.11, Haidian District, Beijing, 100048, China.
- Key Laboratory of Cleaner Production and Comprehensive Utilization of Resources, China National Light Industry, Beijing Technology and Business University, Beijing, China.
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Krajczewski J, Rucińska K, Townley HE, Kudelski A. Role of various nanoparticles in photodynamic therapy and detection methods of singlet oxygen. Photodiagnosis Photodyn Ther 2019; 26:162-178. [DOI: 10.1016/j.pdpdt.2019.03.016] [Citation(s) in RCA: 61] [Impact Index Per Article: 10.2] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 12/15/2018] [Revised: 03/18/2019] [Accepted: 03/22/2019] [Indexed: 01/10/2023]
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Miyagawa M, Aoki T, Seki R, Shibusawa A, Tanaka H. Synthesis of Truffle-shaped Oxide-free Cu Nanoparticles under Atmospheric Conditions with the Aid of Photocatalytic TiO2. CHEM LETT 2017. [DOI: 10.1246/cl.170564] [Citation(s) in RCA: 2] [Impact Index Per Article: 0.3] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 01/04/2023]
Affiliation(s)
- Masaya Miyagawa
- Department of Applied Chemistry, Faculty of Science and Engineering, Chuo University, 1-13-27 Kasuga, Bunkyo-ku, Tokyo 112-8551
| | - Takuya Aoki
- Department of Applied Chemistry, Faculty of Science and Engineering, Chuo University, 1-13-27 Kasuga, Bunkyo-ku, Tokyo 112-8551
| | - Ryoya Seki
- Department of Applied Chemistry, Faculty of Science and Engineering, Chuo University, 1-13-27 Kasuga, Bunkyo-ku, Tokyo 112-8551
| | - Akane Shibusawa
- Department of Applied Chemistry, Faculty of Science and Engineering, Chuo University, 1-13-27 Kasuga, Bunkyo-ku, Tokyo 112-8551
| | - Hideki Tanaka
- Department of Applied Chemistry, Faculty of Science and Engineering, Chuo University, 1-13-27 Kasuga, Bunkyo-ku, Tokyo 112-8551
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Nosaka Y, Nosaka AY. Generation and Detection of Reactive Oxygen Species in Photocatalysis. Chem Rev 2017; 117:11302-11336. [DOI: 10.1021/acs.chemrev.7b00161] [Citation(s) in RCA: 1691] [Impact Index Per Article: 211.4] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 01/11/2023]
Affiliation(s)
- Yoshio Nosaka
- Department of Materials Science
and Technology, Nagaoka University of Technology Nagaoka 940-2188, Japan
| | - Atsuko Y. Nosaka
- Department of Materials Science
and Technology, Nagaoka University of Technology Nagaoka 940-2188, Japan
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Khataee A, Rad TS, Fathinia M. The role of clinoptilolite nanosheets in catalytic ozonation process: Insights into the degradation mechanism, kinetics and the toxicity. J Taiwan Inst Chem Eng 2017. [DOI: 10.1016/j.jtice.2017.05.004] [Citation(s) in RCA: 12] [Impact Index Per Article: 1.5] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/15/2022]
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Mohapatra L, Patra D, Parida K, Zaidi SJ. Enhanced Photocatalytic Activity of a Molybdate-Intercalated Iron-Based Layered Double Hydroxide. Eur J Inorg Chem 2017. [DOI: 10.1002/ejic.201601191] [Citation(s) in RCA: 29] [Impact Index Per Article: 3.6] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/10/2022]
Affiliation(s)
| | | | - Kulamani Parida
- Centre for Nano Science and Nano Technology; ITER; Siksha “O” Anusandhan University; India
| | - Syed Javaid Zaidi
- Center for Advanced Materials (CAM); Qatar University (QU); Doha Qatar
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11
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Jafari S, Mohammadi MR, Madaah Hosseini HR. Impact of Morphology and Nitrogen and Carbon Codoping on Photocatalytic Activity of TiO2 as Environmental Catalysts. Ind Eng Chem Res 2016. [DOI: 10.1021/acs.iecr.6b03053] [Citation(s) in RCA: 9] [Impact Index Per Article: 1.0] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/30/2022]
Affiliation(s)
- S. Jafari
- Department of Materials Science
and Engineering, Sharif University of Technology, Azadi Street, Tehran, Iran
| | - M. R. Mohammadi
- Department of Materials Science
and Engineering, Sharif University of Technology, Azadi Street, Tehran, Iran
| | - H. R. Madaah Hosseini
- Department of Materials Science
and Engineering, Sharif University of Technology, Azadi Street, Tehran, Iran
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12
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Miyoshi N, Kundu SK, Tuziuti T, Yasui K, Shimada I, Ito Y. Combination of Sonodynamic and Photodynamic Therapy against Cancer Would Be Effective through Using a Regulated Size of Nanoparticles. NANOSCIENCE AND NANOENGINEERING 2016; 4:1-11. [PMID: 27088115 PMCID: PMC4827930 DOI: 10.13189/nn.2016.040101] [Citation(s) in RCA: 34] [Impact Index Per Article: 3.8] [Reference Citation Analysis] [Abstract] [Key Words] [Grants] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 12/27/2022]
Abstract
Nanoparticles have been used for many functional materials in nano-sciences and photo-catalyzing surface chemistry. The titanium oxide nanoparticles will be useful for the treatment of tumor by laser and/or ultrasound as the sensitizers in nano-medicine. We have studied the combination therapy of photo- and sono-dynamic therapies in an animal tumor model. Oral-administration of two sensitizers titanium oxide, 0.2%-TiO2 nanoparticles for sono-dynamic and 1 mM 5-aminolevulinic acid for photodynamic therapies have resulted in the best combination therapeutic effects for the cancer treatment. Our light microscopic and Raman spectroscopic studies revealed that the titanium nanoparticles were distributed inside the blood vessel of the cancer tissue (1-3 μm sizes). Among these nanoparticles with a broad size distribution, only particular-sized particles could penetrate through the blood vessel of the cancer tissue, while other particles may only exhibit the side effects in the model mouse. Therefore, it may be necessary to separate the optimum size particles. For this purpose we have separated TiO2 nanoparticles by countercurrent chromatography with a flat coiled column (1.6 mm ID) immersed in an ultrasonic bath (42 KHz). Separation was performed with a two-phase solvent system composed of 1-butanol-acetic acid-water at a volume ratio of 4:1:5 at a flow rate of 0.1 ml/min. Countercurrent chromatographic separation yielded fractions containing particle aggregates at 31 and 4400 nm in diameter.
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Affiliation(s)
- N. Miyoshi
- Department of Tumor Pathology, Faculty of Medical Sciences, University of Fukui, Japan
| | - S. K. Kundu
- Department of Tumor Pathology, Faculty of Medical Sciences, University of Fukui, Japan
- Department of Pharmacy, Jahangirnagar University, Bangladesh
| | - T. Tuziuti
- National Institute of Advanced Industrial Science and Technology (NAIST), Japan
| | - K. Yasui
- National Institute of Advanced Industrial Science and Technology (NAIST), Japan
| | - I. Shimada
- Department of Forensic Medicine, Faculty of Medicine, University of Fukui, Japan
| | - Y. Ito
- Laboratory of Bioseparation Technology, Biochemistry and Biophysics Center, National Heart, Lung, and Blood Institute, USA
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Roy Chowdhury P, Bhattacharyya KG. Synthesis and characterization of Co/Ti layered double hydroxide and its application as a photocatalyst for degradation of aqueous Congo Red. RSC Adv 2015; 5:92189-92206. [PMID: 30174825 PMCID: PMC6114096 DOI: 10.1039/c5ra19106h] [Citation(s) in RCA: 40] [Impact Index Per Article: 4.0] [Reference Citation Analysis] [Abstract] [Grants] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 12/17/2022] Open
Abstract
2 : 1 Co/Ti layered double hydroxide (LDH) was synthesized hydrothermally using commercially available Co(NO3)2·6H2O and TiCl4, on a urea template. The high surface area material (~180 m2 g-1) had a narrow band gap (2.67 eV) and shallow and deep trap defect sites. The layered nanomaterial exhibited remarkable semiconductor properties and demonstrated excellent visible light decolourisation efficiency for the anionic dye Congo Red in aqueous medium. The photocatalytic efficiency of the LDH was better than common commercial materials in use such as ZnO, ZnS, NiO, CoO, TiO2 and Degussa P25. The presence of different surface states of defect sites in the LDH was confirmed by PL, EIS and XPS measurements. XRD, DRS, FT-IR, AFM, TEM, SEM/EDX and TG/DTG analyses yielded information about the structural, morphological properties and thermal stability of the LDH. BET N2 adsorption-desorption measurements at 77 K gave surface area and porosity data for the LDH. The surface charge characteristics of the LDH were evaluated with ξ-potential measurements over a wide pH-range in aqueous medium. The photocatalytic behaviour towards decolourisation of the dye was evaluated depending on the reaction variables of pH, LDH amount, initial dye concentration and effects of quenchers, and variation of molar ratios of Co/Ti LDH. The pseudo-first order model satisfactorily described the degradation kinetics of the anionic dye. The photocatalytic mechanistic pathways of the LDH were explained on the basis of an electron-hole (e--h+) hopping conduction model and also photosensitization of the dye. The maximum catalytic efficiency was observed with 15.0 mg of LDH at pH 4 for the anionic Congo Red dye at a concentration 1 × 10-5 M. The LDH was stable even after the fifth catalytic cycle, indicating its remarkable efficiency in potential decolourisation treatments. The dye degradation products were analysed with GC-MS and a reaction mechanism was proposed for the breakdown of the dye to simple and less toxic components.
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Roy Chowdhury P, Bhattacharyya KG. Ni/Ti layered double hydroxide: synthesis, characterization and application as a photocatalyst for visible light degradation of aqueous methylene blue. Dalton Trans 2015; 44:6809-24. [PMID: 25763803 PMCID: PMC6088246 DOI: 10.1039/c5dt00257e] [Citation(s) in RCA: 81] [Impact Index Per Article: 8.1] [Reference Citation Analysis] [Abstract] [Grants] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/21/2022]
Abstract
Visible light responsive 2 : 1 Ni/Ti layered double hydroxide (LDH) was synthesized by a single step hydrothermal route using commercially available Ni(NO3)2·6H2O, TiCl4 and urea. The material exhibited significant absorption in the visible range with a very narrow band gap (2.68 eV). This could be attributed to structural defects as confirmed by diffuse reflectance spectroscopy (DRS), photoluminescence (PL), X-ray diffraction (XRD) and X-ray photoelectron spectroscopy (XPS) measurements. FT-IR, TGA, DTA, DSC, HR-TEM and SEM-EDX measurements yielded information about structural aspects, thermal stability and surface morphology. Surface and pore characteristics of the material were obtained from the BET isotherm for N2 adsorption at 77 K. Zeta potential measurements were used to characterize the electrical properties of the surface while XPS revealed changes in surface states and oxygen deficiencies. The material was found to be an excellent photocatalyst for the degradation of aqueous methylene blue in visible light. The photocatalytic properties of the material were explained on the basis of the narrow band gap, the high surface area and the presence of surface defects. The photocatalytic activity improved in alkaline media [pH 11.0, catalyst load 15 mg in 200 ml dye solution, dye concentration 1 × 10(-6) M (= 0.3198 mg L(-1))] due to the electrostatic attractions between the dye cations and the negative charges on the Ni/Ti LDH surface. The catalytic activity was found to be higher than the common commercial catalysts like ZnO, ZnS, NiO, TiO2 and Degussa P25. The catalytic activity was retained even after five methylene blue degradation cycles, demonstrating that the LDH could be an important addition to the field of wastewater treatment.
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Spectroscopic Studies on Photoelectron Transfer from 2-(furan-2-yl)-1-phenyl-1H-phenanthro[9,10-d]imidazole to ZnO, Cu—doped ZnO and Ag—doped ZnO. J Fluoresc 2014; 24:1447-55. [DOI: 10.1007/s10895-014-1428-6] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 04/21/2014] [Accepted: 07/07/2014] [Indexed: 10/24/2022]
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Baliarsingh N, Parida KM, Pradhan GC. Effects of Co, Ni, Cu, and Zn on Photophysical and Photocatalytic Properties of Carbonate Intercalated MII/Cr LDHs for Enhanced Photodegradation of Methyl Orange. Ind Eng Chem Res 2014. [DOI: 10.1021/ie403769b] [Citation(s) in RCA: 120] [Impact Index Per Article: 10.9] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 12/23/2022]
Affiliation(s)
- N. Baliarsingh
- Colloids & Materials Chemistry Department, CSIR-Institute of Minerals & Materials Technology, Bhubaneswar-751013, Odisha, India
| | - K. M. Parida
- Colloids & Materials Chemistry Department, CSIR-Institute of Minerals & Materials Technology, Bhubaneswar-751013, Odisha, India
| | - G. C. Pradhan
- Department
of Chemistry, Utkal University, Bhubaneswar-751007, Odisha, India
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Li Z, Pan X, Wang T, Wang PN, Chen JY, Mi L. Comparison of the killing effects between nitrogen-doped and pure TiO2 on HeLa cells with visible light irradiation. NANOSCALE RESEARCH LETTERS 2013; 8:96. [PMID: 23433090 PMCID: PMC3599500 DOI: 10.1186/1556-276x-8-96] [Citation(s) in RCA: 19] [Impact Index Per Article: 1.6] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Subscribe] [Scholar Register] [Received: 12/14/2012] [Accepted: 01/29/2013] [Indexed: 05/08/2023]
Abstract
The killing effect of nitrogen-doped titanium dioxide (N-TiO2) nanoparticles on human cervical carcinoma (HeLa) cells by visible light photodynamic therapy (PDT) was higher than that of TiO2 nanoparticles. To study the mechanism of the killing effect, the reactive oxygen species produced by the visible-light-activated N-TiO2 and pure-TiO2 were evaluated and compared. The changes of the cellular parameters, such as the mitochondrial membrane potential (MMP), intracellular Ca2+, and nitrogen monoxide (NO) concentrations after PDT were measured and compared for N-TiO2- and TiO2-treated HeLa cells. The N-TiO2 resulted in more loss of MMP and higher increase of Ca2+ and NO in HeLa cells than pure TiO2. The cell morphology changes with time were also examined by a confocal microscope. The cells incubated with N-TiO2 exhibited serious distortion and membrane breakage at 60 min after the PDT.
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Affiliation(s)
- Zheng Li
- Key Laboratory of Micro and Nano Photonic Structures (Ministry of Education), Department of Optical Science and Engineering, Shanghai Ultra-Precision Optical Manufacturing Engineering Center, Fudan University, 220 Handan Road, 200433, Shanghai, China
| | - Xiaobo Pan
- Key Laboratory of Micro and Nano Photonic Structures (Ministry of Education), Department of Optical Science and Engineering, Shanghai Ultra-Precision Optical Manufacturing Engineering Center, Fudan University, 220 Handan Road, 200433, Shanghai, China
| | - Tianlong Wang
- Key Laboratory of Micro and Nano Photonic Structures (Ministry of Education), Department of Optical Science and Engineering, Shanghai Ultra-Precision Optical Manufacturing Engineering Center, Fudan University, 220 Handan Road, 200433, Shanghai, China
| | - Pei-Nan Wang
- Key Laboratory of Micro and Nano Photonic Structures (Ministry of Education), Department of Optical Science and Engineering, Shanghai Ultra-Precision Optical Manufacturing Engineering Center, Fudan University, 220 Handan Road, 200433, Shanghai, China
| | - Ji-Yao Chen
- State Key Laboratory of Surface Physics, Department of Physics, Fudan University, 220 Handan Road, 200433, Shanghai, China
| | - Lan Mi
- Key Laboratory of Micro and Nano Photonic Structures (Ministry of Education), Department of Optical Science and Engineering, Shanghai Ultra-Precision Optical Manufacturing Engineering Center, Fudan University, 220 Handan Road, 200433, Shanghai, China
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LIAO L, HUANG C, CHEN J, WU Y, HAN Z, PAN H, SHEN S. Synthesis of CuPc/TiO<SUB>2</SUB> Nanotube Composite Materials with Large Surface Area and Their Photocatalytic Activity under Visible Light. CHINESE JOURNAL OF CATALYSIS 2013. [DOI: 10.3724/sp.j.1088.2012.20126] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.1] [Reference Citation Analysis] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 11/25/2022]
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19
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Fei Yin Z, Wu L, Gui Yang H, Hua Su Y. Recent progress in biomedical applications of titanium dioxide. Phys Chem Chem Phys 2013; 15:4844-58. [DOI: 10.1039/c3cp43938k] [Citation(s) in RCA: 354] [Impact Index Per Article: 29.5] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 12/13/2022]
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Yang X, Yan Z, Jiang L, Wang X, Zheng K, Wang Y, Li Q, Wang J. Synthesis and Photocatalysis of AL Doped CdS Templated by Non-Surfactant Hypocrellins. ACTA ACUST UNITED AC 2013. [DOI: 10.1016/j.proenv.2013.04.077] [Citation(s) in RCA: 6] [Impact Index Per Article: 0.5] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 10/26/2022]
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21
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Mohapatra L, Parida K. Zn–Cr layered double hydroxide: Visible light responsive photocatalyst for photocatalytic degradation of organic pollutants. Sep Purif Technol 2012. [DOI: 10.1016/j.seppur.2011.10.028] [Citation(s) in RCA: 95] [Impact Index Per Article: 7.3] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 10/15/2022]
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22
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Castro CA, Osorio P, Sienkiewicz A, Pulgarin C, Centeno A, Giraldo SA. Photocatalytic production of 1O2 and *OH mediated by silver oxidation during the photoinactivation of Escherichia coli with TiO2. JOURNAL OF HAZARDOUS MATERIALS 2012; 211-212:172-181. [PMID: 21940102 DOI: 10.1016/j.jhazmat.2011.08.076] [Citation(s) in RCA: 17] [Impact Index Per Article: 1.3] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 04/10/2011] [Revised: 07/16/2011] [Accepted: 08/29/2011] [Indexed: 05/31/2023]
Abstract
Ag loaded TiO(2) was applied in the photocatalytic inactivation of Escherichia coli under ultraviolet (UV) and visible (Vis) light irradiations. Ag enhanced the TiO(2) photodisinfecting effect under Vis irradiation promoting the formation of singlet oxygen and hydroxyl radicals as identified by EPR analyses. Ag nanoparticles, determined on TEM analyses, undergo an oxidation process on the TiO(2)'s surface under UV or Vis irradiation as observed by XPS. In particular, UV pre-irradiation of the material totally diminished its photodisinfection activity under a subsequent Vis irradiation test. Under UV, photodegradation of dichloroacetic acid (DCA), attributed to photoproduced holes in TiO(2), was inhibited by the presence of Ag suggesting that oxidation of Ag(0) to Ag(+) and Ag(2+) is faster than the oxidative path of the TiO(2)'s holes on DCA molecules. Furthermore, photoassisted increased of Ag(+) concentration on TiO(2)'s surface enhances the bacteriostatic activity of the material in dark periods. Indeed, this latter dark contact of Ag(+)-TiO(2) and E. coli seems to induce recovering of the Vis light photoactivity promoted by the surface Ag photoactive species.
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Affiliation(s)
- Camilo A Castro
- Centro de Investigaciones en Catálisis, Escuela de Ingeniería Química, Universidad Industrial de Santander (UIS), A.A. 678, Bucaramanga, Colombia
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23
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Li Z, Mi L, Wang PN, Chen JY. Study on the visible-light-induced photokilling effect of nitrogen-doped TiO2 nanoparticles on cancer cells. NANOSCALE RESEARCH LETTERS 2011; 6:356. [PMID: 21711880 PMCID: PMC3211446 DOI: 10.1186/1556-276x-6-356] [Citation(s) in RCA: 28] [Impact Index Per Article: 2.0] [Reference Citation Analysis] [Abstract] [Track Full Text] [Subscribe] [Scholar Register] [Received: 01/19/2011] [Accepted: 04/21/2011] [Indexed: 05/23/2023]
Abstract
Nitrogen-doped TiO2 (N-TiO2) nanoparticles were prepared by calcining the anatase TiO2 nanoparticles under ammonia atmosphere. The N-TiO2 showed higher absorbance in the visible region than the pure TiO2. The cytotoxicity and visible-light-induced phototoxicity of the pure- and N-TiO2 were examined for three types of cancer cell lines. No significant cytotoxicity was detected. However, the visible-light-induced photokilling effects on cells were observed. The survival fraction of the cells decreased with the increased incubation concentration of the nanoparticles. The cancer cells incubated with N-TiO2 were killed more effectively than that with the pure TiO2. The reactive oxygen species was found to play an important role on the photokilling effect for cells. Furthermore, the intracellular distributions of N-TiO2 nanoparticles were examined by laser scanning confocal microscopy. The co-localization of N-TiO2 nanoparticles with nuclei or Golgi complexes was observed. The aberrant nuclear morphologies such as micronuclei were detected after the N-TiO2-treated cells were irradiated by the visible light.
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Affiliation(s)
- Zheng Li
- Key Laboratory of Micro and Nano Photonic Structures (Ministry of Education), Department of Optical Science and Engineering, Fudan University, Shanghai 200433, China
| | - Lan Mi
- Key Laboratory of Micro and Nano Photonic Structures (Ministry of Education), Department of Optical Science and Engineering, Fudan University, Shanghai 200433, China
| | - Pei-Nan Wang
- Key Laboratory of Micro and Nano Photonic Structures (Ministry of Education), Department of Optical Science and Engineering, Fudan University, Shanghai 200433, China
| | - Ji-Yao Chen
- Surface Physics Laboratory (National Key Laboratory), Department of Physics, Fudan University, Shanghai 200433, China
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24
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Paramaguru G, Solomon RV, Venuvanalingam P, Renganathan R. Spectroscopic Studies on TiO2 Enhanced Binding of Hypocrellin B with DNA. J Fluoresc 2011; 21:1887-95. [DOI: 10.1007/s10895-011-0885-4] [Citation(s) in RCA: 9] [Impact Index Per Article: 0.6] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 12/10/2010] [Accepted: 03/23/2011] [Indexed: 11/28/2022]
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25
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Diaz-Uribe CE, Daza MC, Martínez F, Páez-Mozo EA, Guedes CL, Di Mauro E. Visible light superoxide radical anion generation by tetra(4-carboxyphenyl)porphyrin/TiO2: EPR characterization. J Photochem Photobiol A Chem 2010. [DOI: 10.1016/j.jphotochem.2010.08.013] [Citation(s) in RCA: 72] [Impact Index Per Article: 4.8] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 10/19/2022]
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26
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Lei W, Zhou Q, Li Z, Wang X, Zhang B. Photodynamic Activity of Ascorbic Acid-modified TiO2Nanoparticles upon Visible Illumination (>550 nm). CHEM LETT 2009. [DOI: 10.1246/cl.2009.1138] [Citation(s) in RCA: 7] [Impact Index Per Article: 0.4] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/12/2022]
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27
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Hong KH, Sun G. Structures and photoactive properties of poly(styrene-co
-vinylbenzophenone). ACTA ACUST UNITED AC 2008. [DOI: 10.1002/polb.21576] [Citation(s) in RCA: 8] [Impact Index Per Article: 0.5] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/07/2022]
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28
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Kwon BG. Characterization of the hydroperoxyl/superoxide anion radical (HO2/O2−) formed from the photolysis of immobilized TiO2 in a continuous flow. J Photochem Photobiol A Chem 2008. [DOI: 10.1016/j.jphotochem.2008.05.001] [Citation(s) in RCA: 9] [Impact Index Per Article: 0.5] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/16/2022]
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29
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Allison RR, Mota HC, Bagnato VS, Sibata CH. Bio-nanotechnology and photodynamic therapy--state of the art review. Photodiagnosis Photodyn Ther 2008; 5:19-28. [PMID: 19356632 DOI: 10.1016/j.pdpdt.2008.02.001] [Citation(s) in RCA: 112] [Impact Index Per Article: 6.6] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 01/09/2008] [Revised: 01/31/2008] [Accepted: 02/01/2008] [Indexed: 11/29/2022]
Abstract
Photodynamic therapy (PDT) and bio-nanotechnology (NT) show striking similarities in clinical design and mechanistics. The PDT paradigm of photosensitizer application, light activation and singlet oxygen generation does in fact occur on the nanoscale level as does the resultant outcomes. NT has the ability to explain as well as modify each of the critical steps of PDT particularly photosensitizer design and delivery, light source miniaturization and optimization, location and intensity of the photodynamic reaction as well as offering a far greater insight into dosimetry and mechanisms of action. This review will explore the current and potential future interactions and modifications NT may have on PDT.
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Affiliation(s)
- R R Allison
- Brody School of Medicine at ECU, Radiation Oncology, 600 Moye Blvd LJCC172, Greenville, NC 28758, USA
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30
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Capote R, Alonso-Lebrero JL, García F, Brieva A, Pivel JP, González S. Polypodium leucotomos extract inhibits trans-urocanic acid photoisomerization and photodecomposition. JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY 2006; 82:173-9. [PMID: 16388959 DOI: 10.1016/j.jphotobiol.2005.11.005] [Citation(s) in RCA: 38] [Impact Index Per Article: 2.0] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 05/30/2005] [Revised: 09/08/2005] [Accepted: 11/17/2005] [Indexed: 10/25/2022]
Abstract
In this report, we demonstrate a possible molecular mechanism by which a hydrophilic extract of the leaves of the fern Polypodium leucotomos (Fernblock, PL) blocks ultraviolet (UV)-induced skin photodamage. The extract inhibits UVA and UVB light induced photoisomerization of trans-urocanic acid (t-UCA), a common photoreceptor located in the stratum corneum, and also blocks its photodecomposition in the presence of oxidizing reagents such as H2O2, and titanium dioxide (TiO2). PL protects in vitro human fibroblasts from UV-induced death as well. These results suggest the potential of employing the PL extract as a component of sunscreen moistures in order to prevent photodecomposition of t-UCA, to inhibit UV-induced deleterious effects of TiO2 and to protect skin cells and endogenous molecules directly involved in skin immunosurveillance.
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Affiliation(s)
- R Capote
- R&D Department, Industrial Farmacéutica Cantabria, C Arequipa 1 EDIF Ofic 5 planta, IFC, 28043 Madrid, Spain
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31
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Li Y, Leung P, Yao L, Song QW, Newton E. Antimicrobial effect of surgical masks coated with nanoparticles. J Hosp Infect 2005; 62:58-63. [PMID: 16099072 DOI: 10.1016/j.jhin.2005.04.015] [Citation(s) in RCA: 291] [Impact Index Per Article: 14.6] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 11/20/2004] [Accepted: 04/19/2005] [Indexed: 11/23/2022]
Abstract
This study assessed the antimicrobial activity of nanoparticles (consisting of a mixture of silver nitrate and titanium dioxide) and nanoparticle-coated facemasks to protect against infectious agents. The minimum inhibitory concentrations of the nanoparticles against Escherichia coli and Staphylococcus aureus were 1/128 and 1/512, respectively. The antibacterial activity of nanoparticle-coated masks was quantified according to the procedures of AATCC 100-1999. A 100% reduction in viable E. coli and S. aureus was observed in the coated mask materials after 48 h of incubation. Skin irritation was not observed in any of the volunteers who wore the facemasks. Nanoparticles show promise when applied as a coating to the surface of protective clothing in reducing the risk of transmission of infectious agents.
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Affiliation(s)
- Y Li
- Institute of Textiles and Clothing, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, China
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32
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Zhang Y, Fan Y, Sun C, Shen D, Li Y, Li J. Functionalized polydiacetylene-glycolipid vesicles interacted with Escherichia coli under the TiO2 colloid. Colloids Surf B Biointerfaces 2005; 40:137-42. [PMID: 15708502 DOI: 10.1016/j.colsurfb.2004.10.016] [Citation(s) in RCA: 22] [Impact Index Per Article: 1.1] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 10/26/2022]
Abstract
The interaction of the conjugated system between functionalized polydiacetylene-glycolipid vesicles and Escherichia coli was investigated under the bactericidal effect of TiO(2) colloid. With various pre-incubation and irradiation time, controllable bacteria quantity in the presence of TiO(2) colloid was obtained in real time. UV-visible and Raman spectra were utilized to monitor and evaluate the structural transition of bacteria-polydiacetylene-glycolipid vesicles conjugated system under the different conditions, which supplied the detailed information of the transform of the polydiacetylene backbone in real time. Thus controllable conjugated bio-interaction between two bio-interfaces was obtained through the introduction of the third factor and monitored in real time. This would be great aid in fundamental understanding more complex interfaces in the biological environment and would be applicable to the biomedical and biophysical fields.
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Affiliation(s)
- Yuanjian Zhang
- State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, PR China
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Ishay JS, Joseph Z, Galushko D, Ermakov N, Bergman DJ, Barkay Z, Stokroos I, van der Want J. Perovskites in the comb roof base of hornets: Their possible function. Microsc Res Tech 2005; 66:259-69. [PMID: 15940685 DOI: 10.1002/jemt.20168] [Citation(s) in RCA: 2] [Impact Index Per Article: 0.1] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/07/2022]
Abstract
On the ceiling of the Oriental hornet comb cell, there are mineral granules of polycrystalline material known to belong to the group of perovskites. In a comb cell intended to house a worker hornet, the roof base usually carries one or several such perovskite granules containing titanium (Ti), whereas in the roof base of a cell housing a developing queen, there are usually several granules containing a high percentage of silicon (Si), aluminum (Al), calcium (Ca), and iron (Fe), but very little if any Ti. In worker comb cells, Ti usually appears as ilmenite (FeTiO3). Besides documenting the above-mentioned facts, this report discusses possible reasons for the appearance of ilmenite crystals in worker cells only and not in queen cells.
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Affiliation(s)
- J S Ishay
- Department of Physiology and Pharmacology, Sackler Faculty of Medicine, Tel Aviv University, Ramat Aviv 69978, Israel.
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Ma L, Tai H, Li C, Zhang Y, Wang ZH, Ji WZ. Photodynamic inhibitory effects of three perylenequinones on human colorectal carcinoma cell line and primate embryonic stem cell line. World J Gastroenterol 2003; 9:485-90. [PMID: 12632502 PMCID: PMC4621566 DOI: 10.3748/wjg.v9.i3.485] [Citation(s) in RCA: 30] [Impact Index Per Article: 1.4] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Download PDF] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Indexed: 02/06/2023] Open
Abstract
AIM: To investigate the photodynamic inhibitory effects of Elsinochrome A (EA), Hypocrellin A (HA) and Hypocrellin B (HB) on human colorectal carcinoma Hce-8693 cells and rhesus monkey embryonic stem R366.4 cells, via inducing apoptosis.
METHODS: EA, HA and HB were extracted from metabolites of Hypomyces (Fr) Tul.Sp. R366.4 cells or Hce-8693 cells were cultured with different concentrations of EA, HA or HB respectively, irradiated and incubated with fresh medium for 2 h. Cell cycle analysis was performed by flow cytometry (FCM). Data were expressed as means ± SD and analysis of variance and Student’t-test for individual comparisons.
RESULTS: The photodynamic bioactivity of EA was first reported in this study. After irradiation for 5 min, 6 min, 10 min or 20 min, photoactivated EA at lower concentrations, which were 10-7 Mol/L, 10- 6 Mol/L, 10 - 5 Mol/L respectively, had no cytotoxic effects on R366.4 ES cells. Whereas, all of the three perylenequinones could induce apoptosis with a dose-dependent manner when Hce-8693 cells were incubated with photoactivated EA, HA and HB respectively. When Hce-8693 cells were incubated with EA at 10-6 Mol/L and irradiated 5 min, 6 min, 10 min and 20 min respectively, the rates of EA-induced apoptosis were 0, 0, 13.4% and 40.5%. While the rates of HA-induced apoptosis were 29.5%, 32.0%, 40.2% and 22.6%. And the rates of HB-induced apoptosis were 0, 0, 0 and 13.7% respectively. Meanwhile, after 10-5 Mol/L treatment, the rates of EA-induced apoptosis were 32.7%, 19.3%, 26.4% and 52.7%, the rates of HA-induced apoptosis were 47.2%, 39.1%, 45.2% and 56.6%, and the rates of HB-induced apoptosis were 0, 0, 20.0% and 13.9% respectively.
CONCLUSION: EA, HA and HB have significant anti-cancer activity. The order of photodynamic inhibitory effects on tumor cells would be approximately HA > EA > HB. The molecular mechanisms of apoptosis may not be induced by reactive oxygen species and are worth further investigation.
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Affiliation(s)
- Lan Ma
- Graduate School of the Chinese Acedemy of Sciencees, Beijing 100871, China.
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Liu J, Zhao YW, Zhao JQ, Xia AD, Jiang LJ, Wu S, Ma L, Dong YQ, Gu YH. Two-photon excitation studies of hypocrellins for photodynamic therapy. JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY. B, BIOLOGY 2002; 68:156-64. [PMID: 12468211 DOI: 10.1016/s1011-1344(02)00379-2] [Citation(s) in RCA: 54] [Impact Index Per Article: 2.3] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 12/29/2022]
Abstract
The photophysical and photochemical properties of hypocrellins (HA and HB) are examined with two-photon excitations at 800 nm using femtosecond pulses from a Ti:sapphire laser. The two-photon excited fluorescence spectra of HA and HB are very similar to those obtained by one-photon excitation, which may indicate that the two-photon induced photodynamic processes of hypocrellins are similar to one-photon induced photodynamic processes. The two-photon excitation cross sections of HA and HB are measured at 800 nm as about 34.8 x 10(-50) cm(4) s/photon and 21.3 x 10(-50) cm(4) s/photon, respectively. The large two-photon cross sections of both HA and HB, suggest that the hypocrellins can be potential two-photon phototherapeutic agents. As an example for two-photon photodynamic therapy of hypocrellins, we also further examine the cell-damaging effects of HA upon two-photon illumination. Our preliminary results of cell viability test indicate hypocrellins can effectively damage the Hela cells under two-photon illumination.
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Affiliation(s)
- J Liu
- Center for Molecular Science, Institute of Chemistry, Chinese Academy of Science, Beijing 100080, China
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