|
1
|
C M MG, Murugan SK, Bethapudi B, Purusothaman D, Mundkinajeddu D, D'Souza P. Ocimum tenuiflorum extract (HOLIXERTM): Possible effects on hypothalamic-pituitary-adrenal (HPA) axis in modulating stress. PLoS One 2023; 18:e0285012. [PMID: 37141281 PMCID: PMC10159140 DOI: 10.1371/journal.pone.0285012] [Citation(s) in RCA: 2] [Impact Index Per Article: 1.0] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 11/17/2022] [Accepted: 03/12/2023] [Indexed: 05/05/2023] Open
Abstract
Ocimum tenuiflorum is a sacred medicinal plant bestowed with multiple health benefits. This plant is traditionally considered an adaptogen. Many scientific studies have indicated the anti-stress potential of Ocimum tenuiflorum but with higher doses. The present study investigated the effects of HolixerTM (a clinically studied standardized Ocimum tenuiflorum extract) on modulating stress using two in vivo models, namely the swim endurance study in mice and forced swim test in rats. In addition, we explored the mechanism of action of HolixerTM on the HPA axis using two in vitro cell-based assays to check for its inhibitory effect on cortisol release and CRF1 receptor antagonistic activity. Ocimum tenuiflorum extract enhanced the swimming time in mice, reduced the stress-induced increase in immobility time, and prevented the increase in corticosterone in rats subjected to the forced swim test. Further, Ocimum tenuiflorum extract inhibited cortisol release and exhibited a significant CRF1 receptor antagonist activity. Thus, Ocimum tenuiflorum extract was found effective in managing stress, and the effect could be due to the inhibition of cortisol release and the antagonistic effect on the CRF1 receptors.
Collapse
Affiliation(s)
- Mohan Gowda C M
- Department of Biology, R&D Centre, Natural Remedies Private Limited, Bengaluru, Karnataka, India
| | - Sasi Kumar Murugan
- Department of Biology, R&D Centre, Natural Remedies Private Limited, Bengaluru, Karnataka, India
| | - Bharathi Bethapudi
- Department of Biology, R&D Centre, Natural Remedies Private Limited, Bengaluru, Karnataka, India
| | - Divya Purusothaman
- Department of Biology, R&D Centre, Natural Remedies Private Limited, Bengaluru, Karnataka, India
| | - Deepak Mundkinajeddu
- Department of Biology, R&D Centre, Natural Remedies Private Limited, Bengaluru, Karnataka, India
| | - Prashanth D'Souza
- Department of Biology, R&D Centre, Natural Remedies Private Limited, Bengaluru, Karnataka, India
| |
Collapse
|
|
2
|
Joshi MB, Kamath A, Nair AS, Yedehali Thimmappa P, Sriranjini SJ, Gangadharan GG, Satyamoorthy K. Modulation of neutrophil (dys)function by Ayurvedic herbs and its potential influence on SARS-CoV-2 infection. J Ayurveda Integr Med 2022; 13:100424. [PMID: 33746457 PMCID: PMC7962552 DOI: 10.1016/j.jaim.2021.03.006] [Citation(s) in RCA: 7] [Impact Index Per Article: 2.3] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 07/22/2020] [Revised: 12/08/2020] [Accepted: 03/09/2021] [Indexed: 12/15/2022] Open
Abstract
For centuries, traditional medicines of Ayurveda have been in use to manage infectious and non-infectious diseases. The key embodiment of traditional medicines is the holistic system of approach in the management of human diseases. SARS-CoV-2 (COVID-19) infection is an ongoing pandemic, which has emerged as the major health threat worldwide and is causing significant stress, morbidity and mortality. Studies from the individuals with SARS-CoV-2 infection have shown significant immune dysregulation and cytokine overproduction. Neutrophilia and neutrophil to lymphocyte ratio has been correlated to poor outcome due to the disease. Neutrophils, component of innate immune system, upon stimulation expel DNA along with histones and granular proteins to form extracellular traps (NETs). Although, these DNA lattices possess beneficial activity in trapping and eliminating pathogens, NETs may also cause adverse effects by inducing immunothrombosis and tissue damage in diseases including Type 2 Diabetes and atherosclerosis. Tissues of SARS-CoV-2 infected subjects showed microthrombi with neutrophil-platelet infiltration and serum showed elevated NETs components, suggesting large involvement and uncontrolled activation of neutrophils leading to pathogenesis and associated organ damage. Hence, traditional Ayurvedic herbs exhibiting anti-inflammatory and antioxidant properties may act in a manner that might prove beneficial in targeting over-functioning of neutrophils and there by promoting normal immune homeostasis. In the present manuscript, we have reviewed and discussed pathological importance of NETs formation in SARS-CoV-2 infections and discuss how various Ayurvedic herbs can be explored to modulate neutrophil function and inhibit NETs formation in the context of a) anti-microbial activity to enhance neutrophil function, b) immunomodulatory effects to maintain neutrophil mediated immune homeostasis and c) to inhibit NETs mediated thrombosis.
Collapse
Affiliation(s)
- Manjunath B Joshi
- Manipal School of Life Sciences, Manipal Academy of Higher Education, Manipal, 576104, India
| | - Archana Kamath
- Manipal School of Life Sciences, Manipal Academy of Higher Education, Manipal, 576104, India
| | - Aswathy S Nair
- Manipal School of Life Sciences, Manipal Academy of Higher Education, Manipal, 576104, India
| | | | - Sitaram J Sriranjini
- Ramaiah Indic Speciality Ayurveda-Restoration Hospital, MSR Nagar, Mathikere, Bengaluru, 560 054, India
| | - G G Gangadharan
- Ramaiah Indic Speciality Ayurveda-Restoration Hospital, MSR Nagar, Mathikere, Bengaluru, 560 054, India
| | - Kapaettu Satyamoorthy
- Manipal School of Life Sciences, Manipal Academy of Higher Education, Manipal, 576104, India.
| |
Collapse
|
|
3
|
She X, Gao X, Wang K, Yang H, Ma K, Cui B, Xi Z. Effects of noise and low-concentration carbon monoxide exposure on rat immunity. J Occup Health 2021; 63:e12235. [PMID: 34089209 PMCID: PMC8178495 DOI: 10.1002/1348-9585.12235] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.3] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 10/09/2020] [Revised: 04/23/2021] [Accepted: 05/08/2021] [Indexed: 12/18/2022] Open
Abstract
OBJECTIVE To evaluate the immunotoxicity and effects of noise and/or low-concentration carbon monoxide (CO) exposure on immune organs and immune functions in rats. METHODS Male Wistar rats exposed to 98 dB(A) white noise and/or 100 ppm CO 4 h/d for 30 d were used to determine the pathological changes in the thymus and spleen, and variations in leukocyte counts, inflammatory factors, and immunoglobulin (Ig) concentrations. RESULTS The boundaries of the cortex and medulla of the thymus were unclear following noise and combined exposure. The pathological changes in spleen after CO and combined exposure included blurred boundaries of red-pulp and white-pulp, disappearance of normal splenic nodules and neutrophil infiltration. After exposure to noise and in combination, leukocyte and lymphocyte counts decreased significantly. After exposure to low-concentration CO and in combination, serum IgM and IgG levels decreased significantly, but the levels of tumor necrosis factor-α and interferon-γ levels increased significantly. Eosinophils and IgA levels decreased significantly following exposure to noise and/or low concentration of CO, while the level of interleukin-1 increased significantly. Monocytes increased significantly only under noise or CO exposure, but not under combined exposure. CONCLUSIONS Noise and/or low-concentration CO exposure may suppress innate and adaptive immune functions and induce inflammatory responses. Noise exposure mainly affected the innate immune function of rats, whereas low-concentration CO exposure mainly affected adaptive immune functions. Combined exposure presented higher immunotoxicity than noise or CO alone, suggesting that exposure to noise and low-concentration CO in the living and working environments can affect the immune system.
Collapse
Affiliation(s)
- Xiaojun She
- Tianjin Institute of Environmental and Operational Medicine, Tianjin, China
| | - Xiujie Gao
- Tianjin Institute of Environmental and Operational Medicine, Tianjin, China
| | - Kun Wang
- Tianjin Institute of Environmental and Operational Medicine, Tianjin, China
| | - Honglian Yang
- Tianjin Institute of Environmental and Operational Medicine, Tianjin, China
| | - Kefeng Ma
- Tianjin Institute of Environmental and Operational Medicine, Tianjin, China
| | - Bo Cui
- Tianjin Institute of Environmental and Operational Medicine, Tianjin, China
| | - Zhuge Xi
- Tianjin Institute of Environmental and Operational Medicine, Tianjin, China
| |
Collapse
|
|
4
|
Sørnes EØ, Risal A, Manandhar K, Thomas H, Steiner TJ, Linde M. Use of medicinal plants for headache, and their potential implication in medication-overuse headache: Evidence from a population-based study in Nepal. Cephalalgia 2021; 41:561-581. [PMID: 33435708 PMCID: PMC8047708 DOI: 10.1177/0333102420970904] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.3] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 01/03/2023]
Abstract
Background In Nepal, traditional treatment using medicinal plants is popular. Whereas
medication-overuse headache is, by definition, caused by excessive use of
acute headache medication, we hypothesized that medicinal plants, being
pharmacologically active, were as likely a cause. Methods We used data from a cross-sectional, nationwide population-based study, which
enquired into headache and use of medicinal plants and allopathic
medications. We searched the literature for pharmacodynamic actions of the
medicinal plants. Results Of 2100 participants, 1794 (85.4%) reported headache in the preceding year;
161 (7.7%) reported headache on ≥15 days/month, of whom 28 (17.4%) had used
medicinal plants and 117 (72.7%) allopathic medication(s). Of 46 with
probable medication-overuse headache, 87.0% (40/46) were using allopathic
medication(s) and 13.0% (6/46) medicinal plants, a ratio of 6.7:1, higher
than the overall ratio among those with headache of 4.9:1 (912/185). Of 60
plant species identified, 49 were pharmacodynamically active on the central
nervous system, with various effects of likely relevance in
medication-overuse headache causation. Conclusions MPs are potentially a cause of medication-overuse headache, and not to be
seen as innocent in this regard. Numbers presumptively affected in Nepal are
low but not negligible. This pioneering project provides a starting point
for further research to provide needed guidance on use of medicinal plants
for headache.
Collapse
Affiliation(s)
- Elise Øien Sørnes
- Department of Neuromedicine and Movement Science, NTNU Norwegian University of Science and Technology, Trondheim, Norway
| | - Ajay Risal
- Dhulikhel Hospital, Kathmandu University Hospital, Dhulikhel, Kavre, Nepal.,Kathmandu University School of Medical Sciences, Dhulikhel, Kavre, Nepal
| | - Kedar Manandhar
- Dhulikhel Hospital, Kathmandu University Hospital, Dhulikhel, Kavre, Nepal.,Kathmandu University School of Medical Sciences, Dhulikhel, Kavre, Nepal
| | - Hallie Thomas
- Department of Neuromedicine and Movement Science, NTNU Norwegian University of Science and Technology, Trondheim, Norway
| | - Timothy J Steiner
- Department of Neuromedicine and Movement Science, NTNU Norwegian University of Science and Technology, Trondheim, Norway.,Division of Brain Sciences, Imperial College London, London, UK
| | - Mattias Linde
- Department of Neuromedicine and Movement Science, NTNU Norwegian University of Science and Technology, Trondheim, Norway
| |
Collapse
|
|
5
|
Abouee-Mehrizi A, Rasoulzadeh Y, Kazemi T, Mesgari-Abbasi M. Inflammatory and immunological changes caused by noise exposure: A systematic review. JOURNAL OF ENVIRONMENTAL SCIENCE AND HEALTH. PART C, TOXICOLOGY AND CARCINOGENESIS 2020; 38:61-90. [PMID: 32397946 DOI: 10.1080/26896583.2020.1715713] [Citation(s) in RCA: 8] [Impact Index Per Article: 1.6] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 06/11/2023]
Abstract
Today, due to the growth of industries and spread of the use of various instruments and devices that produce high noise levels, it is necessary to pay more attention to the effects of exposure to noise on organs and tissues in the body. The importance of the immune system in fighting external and pathogenic factors has raised the need to consider external factors (such as harmful physical factors) and make efforts to avoid producing them. In this systematic review, 811 potentially relevant studies were found in Google Scholar, PubMed, and Web of Science databases, of which 32 different English-written articles were included in the study. The method of searching and systematically reviewing articles was based on the assessment tool of the multiple systematic reviews (AMSTAR) method. The results of this study suggested that noise could affect the function of the immune system and its components by affecting other systems and organs of the body, including the central nervous system, auditory system, circulatory system, and endocrine gland. Moreover, it can be hypothesized that noise affects immune system by producing the NADPH oxidase (Nox) and reactive oxygen species (ROS).
Collapse
Affiliation(s)
- Amirreza Abouee-Mehrizi
- Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran
- Department of Occupational Health Engineering, Faculty of Health, Tabriz University of Medical Sciences, Tabriz, Iran
| | - Yahya Rasoulzadeh
- Department of Occupational Health Engineering, Faculty of Health, Tabriz University of Medical Sciences, Tabriz, Iran
| | - Tohid Kazemi
- Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
| | - Mehran Mesgari-Abbasi
- Drug Applied Research Center (DARC), Tabriz University of Medical Sciences, Tabriz, Iran
| |
Collapse
|
|
6
|
Shimizu T, Torres MP, Chakraborty S, Souchek JJ, Rachagani S, Kaur S, Macha M, Ganti AK, Hauke RJ, Batra SK. Holy Basil leaf extract decreases tumorigenicity and metastasis of aggressive human pancreatic cancer cells in vitro and in vivo: potential role in therapy. Cancer Lett 2013; 336:270-80. [PMID: 23523869 DOI: 10.1016/j.canlet.2013.03.017] [Citation(s) in RCA: 18] [Impact Index Per Article: 1.5] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 02/08/2013] [Revised: 03/12/2013] [Accepted: 03/14/2013] [Indexed: 10/27/2022]
Abstract
There is an urgent need to develop alternative therapies against lethal pancreatic cancer (PC). Ocimum sanctum ("Holy Basil") has been used for thousands of years in traditional Indian medicine, but its anti-tumorigenic effect remains largely unexplored. Here, we show that extracts of O. sanctum leaves inhibit the proliferation, migration, invasion, and induce apoptosis of PC cells in vitro. The expression of genes that promote the proliferation, migration and invasion of PC cells including activated ERK-1/2, FAK, and p65 (subunit of NF-κB), was downregulated in PC cells after O. sanctum treatment. Intraperitoneal injections of the aqueous extract significantly inhibited the growth of orthotopically transplanted PC cells in vivo (p<0.05). Genes that inhibit metastasis (E-cadherin) and induce apoptosis (BAD) were significantly upregulated in tumors isolated from mice treated with O. sanctum extracts, while genes that promote survival (Bcl-2 and Bcl-xL) and chemo/radiation resistance (AURKA, Chk1 and Survivin) were downregulated. Overall, our study suggests that leaves of O. sanctum could be a potential source of novel anticancer compounds in the future.
Collapse
Affiliation(s)
- Tomohiro Shimizu
- Department of Biochemistry and Molecular Biology, University of Nebraska Medical Center, Omaha, NE 68198-5870, USA
| | | | | | | | | | | | | | | | | | | |
Collapse
|
|
7
|
Goel A, Singh DK, Kumar S, Bhatia AK. Immunomodulating property of Ocimum sanctum by regulating the IL-2 production and its mRNA expression using rat's splenocytes. ASIAN PAC J TROP MED 2010. [DOI: 10.1016/s1995-7645(10)60021-1] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.1] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 10/19/2022] Open
|
|
8
|
Pattanayak P, Behera P, Das D, Panda SK. Ocimum sanctum Linn. A reservoir plant for therapeutic applications: An overview. Pharmacogn Rev 2010; 4:95-105. [PMID: 22228948 PMCID: PMC3249909 DOI: 10.4103/0973-7847.65323] [Citation(s) in RCA: 165] [Impact Index Per Article: 11.0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 03/10/2010] [Revised: 03/18/2010] [Accepted: 07/10/2010] [Indexed: 11/23/2022] Open
Abstract
The medicinal plants are widely used by the traditional medicinal practitioners for curing various diseases in their day to day practice. In traditional system of medicine, different parts (leaves, stem, flower, root, seeds and even whole plant) of Ocimum sanctum Linn. have been recommended for the treatment of bronchitis, malaria, diarrhea, dysentery, skin disease, arthritis, eye diseases, insect bites and so on. The O. sanctum L. has also been suggested to possess anti-fertility, anticancer, antidiabetic, antifungal, antimicrobial, cardioprotective, analgesic, antispasmodic and adaptogenic actions. Eugenol (1-hydroxy-2-methoxy-4-allylbenzene), the active constituents present in O. sanctum L. have been found to be largely responsible for the therapeutic potentials. The pharmacological studies reported in the present review confirm the therapeutic value of O. sanctum L. The results of the above studies support the use of this plant for human and animal disease therapy and reinforce the importance of the ethno-botanical approach as a potential source of bioactive substances.
Collapse
Affiliation(s)
| | - Pritishova Behera
- Jeypore College of Pharmacy, Jeypore (K), Bhubaneswar, Orissa, India
| | - Debajyoti Das
- School of Pharmaceutical Sciences, SOA University, Bhubaneswar, Orissa, India
| | - Sangram K. Panda
- Jeypore College of Pharmacy, Jeypore (K), Bhubaneswar, Orissa, India
| |
Collapse
|
|
9
|
Harikrishnan R, Balasundaram C. In vitro and in vivo studies of the use of some medicinal herbals against the pathogen Aeromonas hydrophila in goldfish. JOURNAL OF AQUATIC ANIMAL HEALTH 2008; 20:165-176. [PMID: 18942593 DOI: 10.1577/h05-035.1] [Citation(s) in RCA: 29] [Impact Index Per Article: 1.7] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 05/26/2023]
Abstract
Aeromonas hydrophila is a ubiquitous and opportunistic bacterial pathogen that produces ulcerative dermatitis under stress conditions and inflicts severe losses on global fisheries and fish culture. This study evaluates the antimicrobial potency of aqueous and ethanolic decoction (individual extract) and concoction (mixed extract) of three common medicinal herbs, turmeric Curcuma longa, Tulsi plant Ocimum sanctum, and neem Azadirachta indica, against the in vitro growth of A. hydrophila. Among the decoctions, A. indica exhibited the most potent antibacterial property (P < 0.05) against A. hydrophila. Among the concoctions, both the aqueous and ethanolic triherbal extracts mixed in the ratio of 1:1:1 had higher antibacterial activity (P < 0.05) than the other concoctions and decoctions. Goldfish Carassius auratus (10 +/- 2 g) were challenged with A. hydrophila intramuscularly in the caudal region with two separate doses (days 1 and 3) of 50 microL/fish (1.8 x 10(3) colony-forming units per milliliter). On days 9 (early) and 15 (late) of infection, fish were held in a net and dip treated for 5 min daily in a 1-L solution of 1% aqueous triherbal concoction. Red blood cell (RBC) count, hemoglobin, and hematocrit levels of the infected group were significantly higher (P < 0.05) than those of the control group. In the early treated group, all of the affected profile values returned to near normal, while the late-treated group registered a partial recovery, such as improved RBC count. The derived hematological values, such as mean corpuscular volume, mean corpuscular hemoglobin, and mean corpuscular hemoglobin concentration, of the early and late-treated groups also significantly declined (P < 0.05) but were restored to near normal (P > 0.05) only in the early treated group. The results suggest that dip treatment of A. hydrophila-infected goldfish in an aqueous triherbal concoction had a synergistic restorative effect on the hematological variables.
Collapse
Affiliation(s)
- Ramasamy Harikrishnan
- Phytotherapy Fish Disease Management Unit, Department of Animal Science, Bharathidasan University, Tiruchirapalli 620024, Tamil Nadu, India
| | | |
Collapse
|
|
10
|
Adhvaryu MR, Reddy N, Parabia MH. Effects of four Indian medicinal herbs on Isoniazid-, Rifampicin- and Pyrazinamide-induced hepatic injury and immunosuppression in guinea pigs. World J Gastroenterol 2007; 13:3199-205. [PMID: 17589898 PMCID: PMC4436605 DOI: 10.3748/wjg.v13.i23.3199] [Citation(s) in RCA: 36] [Impact Index Per Article: 2.0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Download PDF] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Indexed: 02/06/2023] Open
Abstract
AIM: To evaluate and compare the hepatoprotective and immunomodulatory effects of Curcuma longa (CL), Ocimum sanctum (OS), Tinospora cordifolia (TC) and Zizyphus mauritiana (ZM) on liver injury and immunosuppression induced by Isoniazid (INH), Rifampicin (RIF) and Pyrazinamide (PZA).
METHODS: Duncan Hartley guinea pigs, weighing 700-1050 g, were treated orally with 50 mg/kg of INH, 100 mg/kg of RIF and 300 mg/Kg of PZA for 21-d. 200 mg/kg (bw) of each herb crude extract was administered to the herb control group and 2-h previous to INH + RIF + PZA (AKT) doses to the Herb + AKT groups. Serum alanine aminotransferase (ALT), aspertate aminotransferase (AST) bilirubin and Alkaline Phosphatase (ALP) were assessed on d 0 and 21 in all the groups. Phagocytic % (P%), Phagocytic Index (PI) and Chemotactic Index (CI) were also measured as immunologic parameters. Histological analysis was carried out to assess injury to the liver.
RESULTS: The AKT treated control group showed hepatotoxicity as judged by elevated serum AST 5-fold, AST/ALT ratio 4-fold, ALP 2-fold and hepatological changes, such as focal necrosis, portal triaditis and steatosis. Immune function was suppressed as judged by decreased P% (51.67 ± 1.68 vs 40.61 ± 1.28, P < 0.01), PI (2.0725 ± 0.05 vs 0.61 ± 0.05, P < 0.001) and CI (1.8525 ± 0.04 vs 0.695 ± 0.07, P < 0.001). All four herb treated groups showed normal liver histology, enzyme levels and increased P%, while PI and CI were enhanced in the TC and ZM treated groups, respectively. CL + AKT, TC + AKT and ZM + AKT showed nearly normal histology with minimal inflammation and microvesicular steatosis, while OS + AKT showed partial protection. Hepatotoxicity was prevented by restricting the rise of AST by 2-fold in CL + AKT and TC + AKT groups and by 3-fold in OS + AKT and ZM + AKT groups, AST/ALT by 2-fold and ALP to normal levels in all four groups. All four herb + AKT groups showed normal to enhanced neutrophil function.
CONCLUSION: All four herbs showed hepatoprotective potential and prevented immunosuppression. CL and TC showed the highest hepatoprotective activity, while TC and ZM showed strong immunostimulatory activity.
Collapse
Affiliation(s)
- Meghna-R Adhvaryu
- Bapalal Vaidya Botanical Research Centre, Department of Biosciences, Veer Narmad South Gujarat University, 110, Nehru Nagar Society, Ichchhanath Road, Surat 395007, India.
| | | | | |
Collapse
|
|
11
|
Samson J, Sheeladevi R, Ravindran R. Oxidative stress in brain and antioxidant activity of Ocimum sanctum in noise exposure. Neurotoxicology 2007; 28:679-85. [PMID: 17379314 DOI: 10.1016/j.neuro.2007.02.011] [Citation(s) in RCA: 25] [Impact Index Per Article: 1.4] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 10/02/2006] [Revised: 01/22/2007] [Accepted: 02/20/2007] [Indexed: 10/23/2022]
Abstract
Noise is a pervasive aspect of many modern communities, work environments and its damaging effects, particularly the production of free radicals are not limited to the auditory organ. The oxidative stress in three discrete brain regions, in wistar strain male albino rats subjected to three different durations of noise exposures (acute, sub-acute and chronic noise stress) and the in vivo as well as the in vitro antioxidant activity of Ocimum sanctum has been analyzed. Broadband white noise (100dB) exposure significantly increased the levels of superoxide dismutase (EC 1.15.1.1), catalase (EC 1.11.1.6), glutathione peroxidase (EC 1.11.1.9), lipid peroxidation, oxidized glutathione (GSSG) and decreased the levels of reduced glutathione (GSH), GSH/GSSG ratio. However, administration of ethanolic extract of O. sanctum attenuates the alterations induced by noise exposure. The antioxidant activity of O. sanctum is also evident from its effectiveness in scavenging the free radicals in a dose dependent manner in the herbal antioxidant assays. The results indicate that adaptation to noise does not occur in the brain regions even after 30 days of noise exposure. The abundance of phytochemicals such as phenolics and flavanoids in O. sanctum may be held responsible for its attenuating activity. Therefore, this study indicates that O. sanctum has the potential for further evaluation as an ideal antioxidant for the noise induced oxidative stress.
Collapse
Affiliation(s)
- James Samson
- Department of Physiology, Dr. ALM PG Institute of Basic Medical Sciences, University of Madras, Taramani, Chennai 600 113, India.
| | | | | |
Collapse
|
|
12
|
Harikrishnan R, Balasundaram C. Modern Trends inAeromonas hydrophilaDisease Management with Fish. ACTA ACUST UNITED AC 2007. [DOI: 10.1080/10641260500320845] [Citation(s) in RCA: 58] [Impact Index Per Article: 3.2] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 10/25/2022]
|
|
13
|
Samson J, Sheela Devi R, Ravindran R, Senthilvelan M. Biogenic amine changes in brain regions and attenuating action of Ocimum sanctumin noise exposure. Pharmacol Biochem Behav 2006; 83:67-75. [PMID: 16427690 DOI: 10.1016/j.pbb.2005.12.008] [Citation(s) in RCA: 19] [Impact Index Per Article: 1.0] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Received: 05/10/2005] [Revised: 12/02/2005] [Accepted: 12/09/2005] [Indexed: 12/16/2022]
Abstract
Broadband white noise exposure (100 dB) in wistar strain male albino rats significantly increased the levels of dopamine (DA), serotonin (5-HT) and 5-HT turnover in many of the discrete brain regions (cerebral cortex, cerebellum, hypothalamus, hippocampus, pons-medulla and corpus striatum) during sub-chronic noise exposure (4 h daily for 15 days). In acute (4 h for 1 day) and chronic noise exposures (4 h daily for 30 days) the levels were significantly altered only in certain regions. The turnover study of serotonin clearly indicates that neurotransmitter level alone cannot be an indicator, as in some brain regions the rate of synthesis matched with the degradation in order to maintain the normal levels. The intraperitoneal administration of 70% ethanolic extract of Ocimum sanctum(OS) at the dosage of 100 mg/kg body weight to animals subjected to noise exposure has prevented the noise induced increase in neurotransmitter levels without affecting the normal levels. This indicates that OS can be a probable herbal remedy for noise induced biogenic amine alterations.
Collapse
Affiliation(s)
- J Samson
- Department of Physiology, ALM PG Institute of Basic Medical Sciences, University of Madras, Taramani, Chennai 600 113, India.
| | | | | | | |
Collapse
|
|
14
|
Samson J, Sheela Devi R, Ravindran R, Senthilvelan M. Effect of noise stress on free radical scavenging enzymes in brain. ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY 2005; 20:142-148. [PMID: 21783581 DOI: 10.1016/j.etap.2004.12.059] [Citation(s) in RCA: 22] [Impact Index Per Article: 1.1] [Reference Citation Analysis] [Abstract] [Track Full Text] [Subscribe] [Scholar Register] [Received: 05/04/2004] [Accepted: 12/12/2004] [Indexed: 05/31/2023]
Abstract
The levels of free radical scavenging enzymes in six discrete brain regions (cerebral cortex, cerebellum, midbrain, pons medulla, hippocampus and hypothalamus), after exposure to three different durations of noise stress (broadband white noise, 100dB, 4h/day, in acute: 1 day, sub acute: 15 days and chronic stress: 30 days) was studied in Wister strain male albino rats. Acute noise stress significantly increases the levels of superoxide dismutase (SOD: EC 1.15.1.1), catalase (EC 1.11.1.6), glutathione peroxidase (GPx: EC 1.11.1.9), oxidized glutathione (GSSG) and lipid peroxidation (LPO) and decreases the levels of reduced glutathione (GSH), GSH/GSSG ratio and protein thiols. The free radical scavenging enzymes levels, in sub acute and chronic noise stress also showed marked deviation in certain regions from the controls within the study duration. The results indicate that adaptation to noise stress does not occur immediately in all the brain regions. The noise induced alterations in free radicals may be assumed to serve as a linkage between the environmental noise and the manifestation of multifactorial diseases attributed to noise exposure. Moreover the quantification of the health effects of noise by the alterations in free radicals seems inappropriate in chronic noise stress.
Collapse
Affiliation(s)
- J Samson
- Department of Physiology, Dr. ALM PG Institute of Basic Medical Sciences, University of Madras, Taramani, Chennai 600-113, India
| | | | | | | |
Collapse
|
|
15
|
Sembulingam K, Sembulingam P, Namasivayam A. Effect of Ocimum sanctum Linn on the changes in central cholinergic system induced by acute noise stress. JOURNAL OF ETHNOPHARMACOLOGY 2005; 96:477-482. [PMID: 15619567 DOI: 10.1016/j.jep.2004.09.047] [Citation(s) in RCA: 15] [Impact Index Per Article: 0.8] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 01/30/2004] [Revised: 09/23/2004] [Accepted: 09/23/2004] [Indexed: 05/24/2023]
Abstract
The ethanolic extract from the leaves of Ocimum sanctum Linn was screened for its effects on the noise induced changes in the central cholinergic system of albino rats by investigating the acetylcholine content and acetylcholinesterase activity in discrete areas of brain. Exposure to noise (10 kHz:100 dB) stress for 30 min caused a significant reduction in total acetylcholine content and increase in the activity of acetylcholinesterase in cerebral cortex, corpus striatum, hypothalamus and hippocampus of brain. Pretreatment of the animals with ethanol extract of Ocimum sanctum leaves for 7 days prevented the noise induced changes in these two cholinergic parameters in all the four areas of brain. The results of the study indicate the protective nature of the plant material on the brain tissues against the detrimental effect of noise stress.
Collapse
Affiliation(s)
- K Sembulingam
- Department of Physiology, Sri Ramachandra Medical College and Research Institute (Deemed University), Porur, Chennai 600116, India.
| | | | | |
Collapse
|
|
16
|
Manikandan S, Srikumar R, Jeya Parthasarathy N, Sheela Devi R. Protective Effect of Acorus calamus LINN on Free Radical Scavengers and Lipid Peroxidation in Discrete Regions of Brain against Noise Stress Exposed Rat. Biol Pharm Bull 2005; 28:2327-30. [PMID: 16327175 DOI: 10.1248/bpb.28.2327] [Citation(s) in RCA: 47] [Impact Index Per Article: 2.4] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/22/2022]
Abstract
Exposure to continuous loud noise is a serious health problem due to excess production of oxygen free radicals. In medical research, more attention is paid to the antioxidant properties of medicinal plants to minimize the harmful effects of radicals. The aim of this study was to evaluate the protective effect of both ethyl acetate and methanolic extract of Acorus calamus LINN against noise stress (30 d, 100 dBA/4h/d) induced changes in the rat brain. We measured the activity of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and the levels of reduced glutathione (GSH), vitamin C, vitamin E, protein thiols and lipid peroxidation (LPO) for the evaluation of oxidative stress status in discrete regions of the rat brain like cerebral cortex, cerebellum, pons-medulla, midbrain, hippocampus and hypothalamus. The results indicated that during exposure of noisy environment ROS generation led to increase in corticosterone, LPO and SOD, but decrease in CAT, GPx, GSH, protein thiols, vitamins C and E levels. Both the ethyl acetate and methanolic extract of Acorus calamus protected most of the changes in the rat brain induced by noise-stress.
Collapse
|
|
17
|
Ravindran R, Rathinasamy SD, Samson J, Senthilvelan M. Noise-Stress-Induced Brain Neurotransmitter Changes and the Effect of Ocimum sanctum (Linn) Treatment in Albino Rats. J Pharmacol Sci 2005; 98:354-60. [PMID: 16113498 DOI: 10.1254/jphs.fp0050127] [Citation(s) in RCA: 59] [Impact Index Per Article: 3.0] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 10/25/2022] Open
Abstract
In this modern world, stress and pollution are unavoidable phenomena affecting the body system at various levels. A large number of people are exposed to potentially hazardous noise levels in daily modern life, such as noise from work environments, urban traffic, and household appliances. A variety of studies have suggested an association between noise exposure and the occurrence of disorders involving extra-auditory organs such as disorders of the nervous, endocrine, and cardiovascular systems. In this study, Wistar strain albino rats were subjected to 100 dB broadband white noise, 4 h daily for 15 days. The high-pressure liquid chromatographic estimation of norepinephrine, epinephrine, dopamine, and serotonin in discrete regions of the rat brain indicates that noise stress can alter the brain biogenic amines after 15 days of stress exposure. Ocimum sanctum (OS), a medicinal herb that is widely claimed to posses antistressor activity and used extensively in the Indian system of medicine for a variety of disorders, was chosen for this study. Administration of the 70% ethanolic extract of OS had a normalizing action on discrete regions of brain and controlled the alteration in neurotransmitter levels due to noise stress, emphasizing the antistressor potential of this plant.
Collapse
Affiliation(s)
- Rajan Ravindran
- Department of Physiology, Dr. ALM. Post Graduate Institute of Basic Medical Sciences, University of Madras, Taramani Campus, Chennai - 600 113
| | | | | | | |
Collapse
|
|
18
|
El Abbouyi A, Toumi M, El Hachimi Y, Jossang A. In vitro effects of aqueous seeds extract of Acacia cyanophylla on the opsonized zymosan-induced superoxide anions production by rat polymorphonuclear leukocytes. JOURNAL OF ETHNOPHARMACOLOGY 2004; 91:159-165. [PMID: 15036483 DOI: 10.1016/j.jep.2003.12.005] [Citation(s) in RCA: 6] [Impact Index Per Article: 0.3] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 10/30/2003] [Revised: 12/02/2003] [Accepted: 12/02/2003] [Indexed: 05/24/2023]
Abstract
In vitro studies were carried out in rat pleural polymorphonuclear leukocytes (PMNs) activated by opsonized zymosan (OZ) to investigate the effects of aqueous extract from Acacia cyanophylla seeds on superoxide anions generation. PMNs were collected, after induction of an acute inflammatory reaction, by injection in the rat pleural cavity, of a suspension of calcium pyrophosphate (CaPP) crystals (pleurisy with CaPP) or serum (pleurisy with serum). The results obtained indicate that Acacia cyanophylla aqueous seeds extract had, in vitro, a significant stimulatory effect, in a dose dependent manner, on the PMN superoxide anions generation. It also corrected the diminution of superoxide anions production induced by diclofenac pre-treated PMNs. It could be concluded from the results of this study that the stimulatory properties of Acacia cyanophylla seeds aqueous extract may at least be due to the presence of polyphenols such tannins and/or lignins. Further investigations are needed to determine clearly the mechanisms mediating the generation of superoxide radicals in this phenomenon.
Collapse
Affiliation(s)
- Ahmed El Abbouyi
- Laboratoire de Biochimie Appliquée et Biotechnologies, BP 20, Faculté des Sciences, El Jadida 24000, Morocco.
| | | | | | | |
Collapse
|
|
19
|
Archana R, Namasivayam A. A comparative study of different crude extracts of Ocimum sanctum on noise stress. Phytother Res 2002; 16:579-80. [PMID: 12237819 DOI: 10.1002/ptr.1018] [Citation(s) in RCA: 5] [Impact Index Per Article: 0.2] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/08/2022]
Abstract
Our previous studies have shown that the ethanolic extract of Ocimum sanctum (OS) leaves was effective in alleviating the noise stress induced changes. Hence in this study, we have investigated the effectiveness of different types of crude OS extracts on some of the stress parameters after noise stress. The results of this study has shown that the active principle responsible for antistressor effect of ethanolic extract is also present in cold homogenised leaf extract of OS also. Hot extracts slightly decrease the potency of the active principle in normalizing corticosteroid level.
Collapse
Affiliation(s)
- R Archana
- Department of Physiology, Dr. ALM Postgraduate Institute of Basic Medical Sciences, University of Madras, Taramani, Chennai - 600 113, India
| | | |
Collapse
|