|
1
|
Ghovvati M, Jain S, Cheng GZ, Kaneko N, Boys JA, Imahori T, De Maeseneer T, Haghniaz R, Cameron RB, Weiss AS, Annabi N. Rapid closure and hemostasis of ruptured soft tissues using a modified human tropoelastin-based sealant in preclinical models. Sci Transl Med 2025; 17:eadr6458. [PMID: 40367193 DOI: 10.1126/scitranslmed.adr6458] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 07/10/2024] [Revised: 12/16/2024] [Accepted: 04/21/2025] [Indexed: 05/16/2025]
Abstract
Treatment of injuries to soft elastic organs is often hindered by challenging anatomical features and limitations of existing sealant materials, which may lack adequate tissue adhesion, elasticity, biocompatibility, and effective hemostatic properties. To address these clinical challenges, we developed an injectable elastic sealant formulated with methacryloyl-modified human recombinant tropoelastin (MeTro) and Laponite silicate nanoplatelets (SNs). We optimized the hydrogel formulation for mechanical properties, adhesion, biocompatibility, and hemostatic properties and used visible light for cross-linking to improve safety. MeTro/SN hydrogels had increased tissue adhesion strength and burst pressure in vitro and ex vivo compared with MeTro alone or commercial sealants. The addition of SNs to the hydrogels facilitated faster blood clotting in vitro without increasing hemolysis. Applied to incisional injuries on rat lungs or aortas, MeTro/SN had burst pressures comparable to those of native tissue and greater than those of MeTro after a 7-day in vivo application. On porcine lungs, MeTro/SN also supported effective lung sealing and burst pressure similar to native lung 14 days after injury sealing. In a rodent tail hemostasis model, MeTro/SN reduced bleeding compared with MeTro. In an injured porcine lung model, early hemostasis was better than the tested commercial sealants. The results demonstrated that MeTro/SN provided effective tissue sealing and promoted hemostasis in a time frame that minimized blood loss without causing a major inflammatory response. These findings highlight the translational potential of our engineered sealant with biomimetic mechanics, durable tissue adhesion, and rapid hemostasis as a multipronged approach for the sealing and repair of traumatic injuries to soft organs.
Collapse
Affiliation(s)
- Mahsa Ghovvati
- Department of Chemical and Biomolecular Engineering, University of California, Los Angeles, Los Angeles, CA 90095, USA
- Department of Radiological Sciences, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA 90095, USA
| | - Saumya Jain
- Department of Chemical and Biomolecular Engineering, University of California, Los Angeles, Los Angeles, CA 90095, USA
| | - George Z Cheng
- Division of Pulmonary, Critical Care, and Sleep Medicine, University of California, San Diego, La Jolla, CA 92093, USA
| | - Naoki Kaneko
- Department of Radiological Sciences, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA 90095, USA
| | - Joshua A Boys
- Division of Cardiothoracic Surgery, Department of Surgery, University of California, San Diego, La Jolla, CA 92093, USA
| | - Taichiro Imahori
- Department of Radiological Sciences, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA 90095, USA
| | - Tess De Maeseneer
- Department of Chemical and Biomolecular Engineering, University of California, Los Angeles, Los Angeles, CA 90095, USA
| | - Reihaneh Haghniaz
- Terasaki Institute for Biomedical Innovation (TIBI), Los Angeles, CA 90024, USA
| | - Robert B Cameron
- Division of Thoracic Surgery, Department of Surgery, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095, USA
- Division of Thoracic Surgery, Department of Surgery and Perioperative Care, West Los Angeles VA Medical Center, Los Angeles, CA 90095, USA
| | - Anthony S Weiss
- Charles Perkins Centre, University of Sydney, Camperdown, NSW 2006, Australia
- School of Life and Environmental Sciences, University of Sydney, Camperdown, NSW 2006, Australia
- Sydney Nano Institute, University of Sydney, Camperdown, NSW 2006, Australia
| | - Nasim Annabi
- Department of Chemical and Biomolecular Engineering, University of California, Los Angeles, Los Angeles, CA 90095, USA
- Department of Bioengineering, University of California, Los Angeles, Los Angeles, CA 90095, USA
| |
Collapse
|
|
2
|
Loffroy R, Guillen K, Chevallier O, Fouad M, Couloumy E, Dencausse A, Robert P, Catoen S, Salsac AV, Aho-Glele SL, Comby PO. Micro-CT and histological assessment of renal arterial embolization with Glubran®2 cyanoacrylate: a medium-term follow-up study in a rabbit model. CVIR Endovasc 2025; 8:33. [PMID: 40261554 PMCID: PMC12014886 DOI: 10.1186/s42155-025-00549-8] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 03/17/2025] [Accepted: 04/07/2025] [Indexed: 04/24/2025] Open
Abstract
BACKGROUND Cyanoacrylate glues are widely used in interventional radiology as effective embolic agents due to their rapid polymerization and ability to achieve vessel occlusion. Nonetheless, concern remains regarding cast stability and potential recanalization over time. This study used multiple modalities to evaluate the medium-term outcomes of Glubran®2 glue (methacryloxysulfolane and N butyl cyanoacrylate) embolisation in a rabbit renal-artery model. METHODS The left renal arteries of six rabbits were embolized with 12.5% or 25% Glubran®2. In-vivo micro-CT scans were performed immediately after embolisation (M0) and ex-vivo scans and a histological assessment were done at one month (M1). Magnetic resonance imaging (MRI) was done at M1 to assess arterial occlusion and parenchymal changes. Quantitative and semi-quantitative parameters reflecting glue distribution, cast integrity, and tissue response were analysed. Statistical comparisons used non-parametric tests. RESULTS All six embolisations were completed without complications. Micro-CT at M1 revealed significant cast resorption and fragmentation with both concentrations, but with no evidence of arterial recanalization. MRI and histology confirmed the persistent vascular occlusion with chronic ischemic changes in the renal parenchyma. Compensatory neovascularization from the renal capsule was observed, with no significant differences in histological inflammation between the two concentrations. Glue casts remained within the arterial lumens and were often surrounded by granulomatous inflammation. CONCLUSIONS Glubran®2 was effective for renal artery embolisation, even at a low concentration of 12.5%: despite partial cast resorption, the arteries remained occluded. Micro-CT proved to be a powerful tool for assessing changes in glue casts. Longer-term studies are warranted to further assess vascular remodelling and occlusion durability.
Collapse
Affiliation(s)
- Romaric Loffroy
- Department of Vascular and Interventional Radiology, Image-Guided Therapy Center, François-Mitterrand University Hospital, Dijon, 21079, France.
- ICMUB Laboratory, UMR CNRS 6302, Université de Bourgogne, Dijon, 210000, France.
| | - Kévin Guillen
- Department of Vascular and Interventional Radiology, Image-Guided Therapy Center, François-Mitterrand University Hospital, Dijon, 21079, France
| | - Olivier Chevallier
- Department of Vascular and Interventional Radiology, Image-Guided Therapy Center, François-Mitterrand University Hospital, Dijon, 21079, France
- ICMUB Laboratory, UMR CNRS 6302, Université de Bourgogne, Dijon, 210000, France
| | - Mohamed Fouad
- Department of Vascular and Interventional Radiology, Image-Guided Therapy Center, François-Mitterrand University Hospital, Dijon, 21079, France
| | - Emilie Couloumy
- R&D, Guerbet Research, Roissy Charles-de-Gaulle, 95943, France
| | - Anne Dencausse
- R&D, Guerbet Research, Roissy Charles-de-Gaulle, 95943, France
| | - Philippe Robert
- R&D, Guerbet Research, Roissy Charles-de-Gaulle, 95943, France
| | - Sarah Catoen
- R&D, Guerbet Research, Roissy Charles-de-Gaulle, 95943, France
| | - Anne-Virginie Salsac
- Biomechanics and Bioengineering Laboratory, UMR CNRS 7338, Université de Technologie de Compiègne, Compiègne, 60203, France
| | - Serge Ludwig Aho-Glele
- Department of Epidemiology, Statistics and Clinical Research, François-Mitterrand University Hospital, Dijon, 21079, France
| | - Pierre-Olivier Comby
- ICMUB Laboratory, UMR CNRS 6302, Université de Bourgogne, Dijon, 210000, France
- Department of Neuroradiology and Emergency Radiology, Image-Guided Therapy Center, François-Mitterrand University Hospital, Dijon, 21079, France
| |
Collapse
|
|
3
|
Hu X, Chen J, Yan Z, Nie D, Guan F, Shi C, Lin N. Multifunctional Silk Fibroin Hydrogels with Strong Adhesion for Tissue Sealing and Wearable Electronic Sensors. ACS APPLIED MATERIALS & INTERFACES 2025; 17:16453-16467. [PMID: 40059406 DOI: 10.1021/acsami.4c18548] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 03/21/2025]
Abstract
Multifunctional hydrogels with excellent adhesion, biodegradability, and conductivity are essential for overcoming the obstacles of postoperative secondary injury, flexible sensing instability, and so on. Herein, we develop a multifunctional silk fibroin (SF) hydrogel modified with poly(acrylic acid). Owing to the stable chemical cross-linking network and the abundant carboxylic acid groups of the SF network, the SF hydrogel exhibits a high tensile strength of 74.34 kPa due to sufficient cohesion and interfacial interactions. Additionally, the tensile strain reaches a maximum of 414.6%, the compressive strength is 0.9 MPa, and the shear adhesive strength for pig skin tissues is as high as 64 kPa. Compared with most hydrogels, our multifunctional SF hydrogel with a low swelling ratio provides excellent adhesion, biodegradation, and conductivity, which shows advantages in terms of invasive tissue sealing. The use of self-adhesive SF hydrogels as conductive hydrogels in flexible sensors also benefits the collection of physiological electricity and human motion signals in the field of wearable and implantable electronic devices.
Collapse
Affiliation(s)
- Xinling Hu
- Research Institution for Biomimetics and Soft Matter, The Higher Educational Key Laboratory for Biomedical Engineering of Fujian Province, Research Center of Biomedical Engineering of Xiamen, Fujian Key Laboratory of Advanced Materials, Department of Biomaterials, College of Materials, Shenzhen Research Institute of Xiamen University, Xiamen University, 422 Siming Nan Road, Xiamen 361005, People's Republic of China
| | - Jitian Chen
- Research Institution for Biomimetics and Soft Matter, The Higher Educational Key Laboratory for Biomedical Engineering of Fujian Province, Research Center of Biomedical Engineering of Xiamen, Fujian Key Laboratory of Advanced Materials, Department of Biomaterials, College of Materials, Shenzhen Research Institute of Xiamen University, Xiamen University, 422 Siming Nan Road, Xiamen 361005, People's Republic of China
| | - Zijian Yan
- Research Institution for Biomimetics and Soft Matter, The Higher Educational Key Laboratory for Biomedical Engineering of Fujian Province, Research Center of Biomedical Engineering of Xiamen, Fujian Key Laboratory of Advanced Materials, Department of Biomaterials, College of Materials, Shenzhen Research Institute of Xiamen University, Xiamen University, 422 Siming Nan Road, Xiamen 361005, People's Republic of China
| | - Du Nie
- Research Institution for Biomimetics and Soft Matter, The Higher Educational Key Laboratory for Biomedical Engineering of Fujian Province, Research Center of Biomedical Engineering of Xiamen, Fujian Key Laboratory of Advanced Materials, Department of Biomaterials, College of Materials, Shenzhen Research Institute of Xiamen University, Xiamen University, 422 Siming Nan Road, Xiamen 361005, People's Republic of China
| | - Fuwang Guan
- College of Textiles and Apparel, Quanzhou Normal University, No. 398, East Sea Street, Quanzhou City, Fujian Province 362000, People's Republic of China
| | - Chenyang Shi
- Physical Sciences Division, Pacific Northwest National Laboratory, Richland, State of Washington 99354, United States
| | - Naibo Lin
- Research Institution for Biomimetics and Soft Matter, The Higher Educational Key Laboratory for Biomedical Engineering of Fujian Province, Research Center of Biomedical Engineering of Xiamen, Fujian Key Laboratory of Advanced Materials, Department of Biomaterials, College of Materials, Shenzhen Research Institute of Xiamen University, Xiamen University, 422 Siming Nan Road, Xiamen 361005, People's Republic of China
| |
Collapse
|
|
4
|
Ilie-Ene A, Tosa VP, Gherman LM, Pop RM, Hantig LM, Popa CO, Dindelegan GC. New Biocompatible Cyanoacrylate and Polylactic Acid Hemostatic Patch: An In Vivo Proof of Concept Study. MATERIALS (BASEL, SWITZERLAND) 2025; 18:1271. [PMID: 40141554 PMCID: PMC11943873 DOI: 10.3390/ma18061271] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Grants] [Track Full Text] [Subscribe] [Scholar Register] [Received: 02/15/2025] [Revised: 03/02/2025] [Accepted: 03/11/2025] [Indexed: 03/28/2025]
Abstract
BACKGROUND Traumatic injuries cause millions of deaths annually, with intra-abdominal hemorrhage as a leading cause. Achieving hemostasis remains challenging. This study evaluates the hemostatic properties of a novel biocompatible cyanoacrylate-polylactic acid patch. METHODS Thirty-six male Wistar rats were randomized into three groups (control, study, and TachoSil®) and underwent a standardized liver resection. The hemostasis times were recorded, and blood samples were drawn to assess hemoglobin, hematocrit, and cytokine levels (IL-6 and TNF-α) using validated assays. RESULTS In the study group, a median time to hemostasis of 94 s was recorded, compared with 256 s for electrocautery (p < 0.001) and 120 s for TachoSil® (p = 0.010). Hemoglobin levels significantly decreased postoperatively in all groups, with the TachoSil® group showing the lowest median value (from 15.4 g/dL to 11.9 g/dL, p = 0.006). Furthermore, only the TachoSil® group exhibited a marked inflammatory response with a significant rise in IL-6 (from 15.6 to 26.8 pg/mL, p = 0.04) and TNF-α (from 9.8 to 28.6 pg/mL, p = 0.01). CONCLUSIONS The results demonstrated that the novel cyanoacrylate-polylactic acid patch achieved rapid hemostasis and was associated with reduced inflammatory responses compared with both electrocautery and TachoSil®. These findings suggest that this hybrid material may offer a safer and more effective hemostatic alternative.
Collapse
Affiliation(s)
- Alexandru Ilie-Ene
- Department of Surgery, “Iuliu Hatieganu” University of Medicine and Pharmacy, 8 Victor Babes Street, 400012 Cluj-Napoca, Romania; (L.-M.H.); (G.C.D.)
| | - Victor Petru Tosa
- Materials Science and Engineering Department, Technical University of Cluj-Napoca, 103-105 Muncii Ave., 400641 Cluj-Napoca, Romania;
| | - Luciana-Madalina Gherman
- Experimental Centre, “Iuliu Haţieganu” University of Medicine and Pharmacy, 6 Louis Pasteur Street, 400349 Cluj-Napoca, Romania;
| | - Raluca Maria Pop
- Pharmacology, Toxicology and Clinical Pharmacology, Department of Morphofunctional Sciences, “Iuliu Hatieganu” University of Medicine and Pharmacy, 8 Victor Babes Street, 400012 Cluj-Napoca, Romania;
| | - Lorena-Maria Hantig
- Department of Surgery, “Iuliu Hatieganu” University of Medicine and Pharmacy, 8 Victor Babes Street, 400012 Cluj-Napoca, Romania; (L.-M.H.); (G.C.D.)
| | - Catalin Ovidiu Popa
- Materials Science and Engineering Department, Technical University of Cluj-Napoca, 103-105 Muncii Ave., 400641 Cluj-Napoca, Romania;
| | - George Calin Dindelegan
- Department of Surgery, “Iuliu Hatieganu” University of Medicine and Pharmacy, 8 Victor Babes Street, 400012 Cluj-Napoca, Romania; (L.-M.H.); (G.C.D.)
| |
Collapse
|
|
5
|
Tomasicchio G, Martines G, Tartaglia N, Buonfantino M, Restini E, Carlucci B, Giove C, Dezi A, Ranieri C, Logrieco G, Vincenti L, Ambrosi A, Altomare DF, De Fazio M, Picciariello A. Suture reinforcement using a modified cyanoacrylate glue to prevent anastomotic leak in colorectal surgery: a prospective multicentre randomized trial : The Rectal Anastomotic seaL (ReAL) trial. Tech Coloproctol 2024; 28:95. [PMID: 39103661 PMCID: PMC11300475 DOI: 10.1007/s10151-024-02967-7] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Grants] [Track Full Text] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Received: 04/21/2024] [Accepted: 06/22/2024] [Indexed: 08/07/2024]
Abstract
BACKGROUND Anastomotic leakage (AL) is the most frequent life-threating complication following colorectal surgery. Several attempts have been made to prevent AL. This prospective, randomized, multicentre trial aimed to evaluate the safety and efficacy of nebulised modified cyanoacrylate in preventing AL after rectal surgery. METHODS Patients submitted to colorectal surgery for carcinoma of the high-medium rectum across five high-volume centres between June 2021 and January 2023 entered the study and were randomized into group A (anastomotic reinforcement with cyanoacrylate) and group B (no reinforcement) and followed up for 30 days. Anastomotic reinforcement was performed via nebulisation of 1 mL of a modified cyanoacrylate glue. Preoperative features and intraoperative and postoperative results were recorded and compared. The study was registered at ClinicalTrials.gov (ID number NCT03941938). RESULTS Out of 152 patients, 133 (control group, n = 72; cyanoacrylate group, n = 61) completed the follow-up. ALs were detected in nine patients (12.5%) in the control group (four grade B and five grade C) and in four patients (6.6%), in the cyanoacrylate group (three grade B and one grade C); however, despite this trend, the differences were not statistically significant (p = 0.36). However, Clavien-Dindo complications grade > 2 were significantly higher in the control group (12.5% vs. 3.3%, p = 0.04). No adverse effects related to the glue application were reported. CONCLUSION The role of modified cyanoacrylate application in AL prevention remains unclear. However its use to seal colorectal anastomoses is safe and could help to reduce severe postoperative complications.
Collapse
Affiliation(s)
- G Tomasicchio
- Department of Precision and Regenerative Medicine and Jonic Area (DiMePRe-J), General Surgery Unit "M. Rubino", University of Bari Aldo Moro, Bari, Italy.
| | - G Martines
- Azienda Ospedaliero Universitaria Policlinico, University of Bari, Piazza G Cesare, 11, 70124, Bari, Italy
| | - N Tartaglia
- Department of Medical and Surgical Sciences, DSMC, University of Foggia, Foggia, Italy
| | - M Buonfantino
- General Surgery Unit, Hospital "San Paolo", Bari, Italy
| | - E Restini
- General Surgery Unit, Hospital "L. Bonomo", Andria, Italy
| | - B Carlucci
- Department of Precision and Regenerative Medicine and Jonic Area (DiMePRe-J), General Surgery Unit "M. Rubino", University of Bari Aldo Moro, Bari, Italy
| | - C Giove
- Department of Precision and Regenerative Medicine and Jonic Area (DiMePRe-J), General Surgery Unit "M. Rubino", University of Bari Aldo Moro, Bari, Italy
| | - A Dezi
- Department of Precision and Regenerative Medicine and Jonic Area (DiMePRe-J), General Surgery Unit "M. Rubino", University of Bari Aldo Moro, Bari, Italy
| | - C Ranieri
- Department of Precision and Regenerative Medicine and Jonic Area (DiMePRe-J), General Surgery Unit "M. Rubino", University of Bari Aldo Moro, Bari, Italy
| | - G Logrieco
- General Regional Hospital "F. Miulli", Acquaviva delle Fonti, Bari, Italy
| | - L Vincenti
- General Surgery Unit, IRCCS "Saverio De Bellis", Castellana Grotte, Italy
| | - A Ambrosi
- Department of Medical and Surgical Sciences, DSMC, University of Foggia, Foggia, Italy
| | - D F Altomare
- Department of Precision and Regenerative Medicine and Jonic Area (DiMePRe-J), General Surgery Unit "M. Rubino", University of Bari Aldo Moro, Bari, Italy
| | - M De Fazio
- Department of Precision and Regenerative Medicine and Jonic Area (DiMePRe-J), General Surgery Unit "M. Rubino", University of Bari Aldo Moro, Bari, Italy
| | - A Picciariello
- Department of Experimental Medicine, University of Salento, Lecce, Italy
| |
Collapse
|
|
6
|
Shen J, Ni L, Zhu C, Jiang C, Zhu W, Bi Y. Efficacy of Endoscopic Tissue Adhesive in Patients with Gastrointestinal Tumor Bleeding. Dig Dis Sci 2024; 69:2559-2566. [PMID: 38662161 PMCID: PMC11258062 DOI: 10.1007/s10620-024-08432-7] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Received: 11/28/2023] [Accepted: 04/08/2024] [Indexed: 04/26/2024]
Abstract
BACKGROUND Gastrointestinal tumors bleeding remains a significantly clinical challenge due to its resistance to conventional endoscopic hemostasis methods. While the efficacy of endoscopic tissue adhesives (ETA) in variceal bleeding has been established, its role in gastrointestinal tumor bleeding (GITB) remains ambiguous. AIMS This study aims to assess the feasibility and effectiveness of ETA in the treatment of GITB. METHODS The study enrolled 30 patients with GITB who underwent hemostasis through Histoacryl® tissue glue injection. Hemostasis success rates, ETA-related adverse events, and re-bleeding rates were evaluated. RESULTS ETA application achieved successful hemostasis at all tumor bleeding sites, with immediate hemostasis observed in all 30 (100.0%) patients. Among the initially hemostasis cases, 5 patients (17.0%) experienced re-bleeding within 30 days, and the 60 day re-bleeding rate was 20.0% (6/30). Expect for one case of vascular embolism, no adverse events related with ETA application were reported. The 6 month survival was 93%. CONCLUSION ETA demonstrated excellent immediate hemostasis success rate in GITB cases and showed promising outcomes in prevention re-bleeding.
Collapse
Affiliation(s)
- Jun Shen
- Department of Gastroenterology, Changzhou Tumor Hospital, Changzhou, 213000, Jiangsu, China
| | - Lingna Ni
- Department of Oncology, Changzhou Tumor Hospital, Changzhou, 213000, Jiangsu, China
| | - Changhong Zhu
- Department of Gastroenterology, Changzhou Tumor Hospital, Changzhou, 213000, Jiangsu, China
| | - Chunying Jiang
- Department of Gastroenterology, Changzhou Tumor Hospital, Changzhou, 213000, Jiangsu, China
| | - Wenyu Zhu
- Department of Oncology, The Affiliated Changzhou No.2 People's Hospital of Nanjing Medical University, Changzhou, 213000, Jiangsu, China
| | - Yanzhi Bi
- Department of Oncology, Changzhou Tumor Hospital, Changzhou, 213000, Jiangsu, China.
| |
Collapse
|
|
7
|
Comby PO, Guillen K, Chevallier O, Couloumy E, Dencausse A, Robert P, Catoen S, Salsac AV, Aho-Glele SL, Loffroy R. Blocked-flow vs. free-flow cyanoacrylate glue embolization: Histological differences in an in vivo rabbit renal artery model. Diagn Interv Imaging 2024; 105:137-143. [PMID: 38284342 DOI: 10.1016/j.diii.2023.10.003] [Citation(s) in RCA: 2] [Impact Index Per Article: 2.0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 07/13/2023] [Revised: 10/05/2023] [Accepted: 10/12/2023] [Indexed: 01/30/2024]
Abstract
PURPOSE The purpose of this in vivo animal study was to compare the acute histological effects on the arterial vessel wall of free-flow vs. blocked-flow embolization with metacryloxysulfolane-n‑butyl cyanoacrylate (MS-NBCA) in several concentrations. MATERIALS AND METHODS A total of 42 rabbit renal arteries were embolized using MS-NBCA mixed with ethiodized oil. The MS-NBCA concentration was 12.5%, 25%, or 50%. All mixtures were injected under both free-flow and blocked-flow conditions. The rabbits were euthanised 30 min after arterial embolization. Arterial-lumen distension, intimal inflammation and necrosis, peri‑arterial edema, and distality of MS-NBCA penetration were assessed histologically. Multivariable regression analyses were performed using a manual backward procedure, with linear, ordinal and logistic regression to search for factors associated with these outcomes RESULTS: Marked or severe dilatation was observed in 36 out of 42 arteries (86%) and marked or transmural intimal arteritis in all 42 arteries (42/42; 100%). Lumen dilatation caused focal vessel-wall flattening, which resulted in intimal necrosis. Multifocal necrosis extending from the intima to the media occurred in 23 out of 42 kidneys (55%) and peri‑arterial edema with multifocal vascular leakage in 19 out of 42 kidneys (45%). At multivariable analysis, blocked-flow MS-NBCA injection was associated with greater severity of vessel-wall lesions, including intimal arteritis (P = 0.003) and intimal necrosis (P = 0.014), compared to free-flow injection. Blocked-flow injection was also associated with peri‑arterial edema (P = 0.008) and greater distality of MS-NBCA penetration (P = 0.001). CONCLUSION Blocked-flow MS-NBCA injection during renal artery embolization is significantly associated with more acute arterial-wall damage and greater distality of glue penetration compared to free-flow injection in a rabbit model. These preliminary findings may have clinical implications, as blocked-flow injection is routinely used to treat specific vascular diseases or malformations in human.
Collapse
Affiliation(s)
- Pierre-Olivier Comby
- Department of Neuroradiology and Emergency Radiology, Image-Guided Therapy Center, François-Mitterrand University Hospital, 21079 Dijon, France; ICMUB Laboratory, UMR CNRS 6302, Université de Bourgogne, 21000 Dijon, France
| | - Kévin Guillen
- ICMUB Laboratory, UMR CNRS 6302, Université de Bourgogne, 21000 Dijon, France; Department of Vascular and Interventional Radiology, Image-Guided Therapy Center, François-Mitterrand University Hospital, 21079 Dijon, France
| | - Olivier Chevallier
- ICMUB Laboratory, UMR CNRS 6302, Université de Bourgogne, 21000 Dijon, France; Department of Vascular and Interventional Radiology, Image-Guided Therapy Center, François-Mitterrand University Hospital, 21079 Dijon, France
| | - Emilie Couloumy
- R&D, Guerbet Research, 95943 Roissy Charles-de-Gaulle, France
| | - Anne Dencausse
- R&D, Guerbet Research, 95943 Roissy Charles-de-Gaulle, France
| | - Philippe Robert
- R&D, Guerbet Research, 95943 Roissy Charles-de-Gaulle, France
| | - Sarah Catoen
- R&D, Guerbet Research, 95943 Roissy Charles-de-Gaulle, France
| | - Anne-Virginie Salsac
- Biomechanics and Bioengineering Laboratory, UMR CNRS 7338, Université de Technologie de Compiègne, 60203 Compiègne, France
| | - Serge Ludwig Aho-Glele
- Department of Epidemiology, Statistics and Clinical Research, François-Mitterrand University Hospital, 21079 Dijon, France
| | - Romaric Loffroy
- ICMUB Laboratory, UMR CNRS 6302, Université de Bourgogne, 21000 Dijon, France; Department of Vascular and Interventional Radiology, Image-Guided Therapy Center, François-Mitterrand University Hospital, 21079 Dijon, France.
| |
Collapse
|
|
8
|
Yu L, Liu Z, Tong Z, Ding Y, Qian Z, Wang W, Mao Z, Ding Y. Sequential-Crosslinking Fibrin Glue for Rapid and Reinforced Hemostasis. ADVANCED SCIENCE (WEINHEIM, BADEN-WURTTEMBERG, GERMANY) 2024; 11:e2308171. [PMID: 38072663 PMCID: PMC10870078 DOI: 10.1002/advs.202308171] [Citation(s) in RCA: 4] [Impact Index Per Article: 4.0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Grants] [Track Full Text] [Download PDF] [Figures] [Subscribe] [Scholar Register] [Received: 11/14/2023] [Indexed: 02/17/2024]
Abstract
Achieving hemostasis effectively is essential for surgical success and excellent patient outcomes. However, it is challenging to develop hemostatic adhesives that are fast-acting, strongly adherent, long-lasting, and biocompatible for treating hemorrhage. In this study, a sequential crosslinking fibrin glue (SCFG) is developed, of which the first network of the fibrin glue forms in situ within 2 s to act as an initial physical barrier and locks the gelatin methacryloyl precursor for tight construction of the second network to enhance wet adhesion and durability for tissues covered with blood. The sequential crosslinking glue can provide large pressures (≈280 mmHg of burst pressure), makes strong (38 kPa of shear strength) and tough (≈60 J m-2 of interfacial toughness) interfaces with wet tissues, and outperforms commercial hemostatic agents and gelatin methacryloyl. SCFG are demonstrated as an effective and safe sealant to enhance the treatment outcomes of bleeding tissues in rat, rabbit, and pig models. The ultrafast gelation, strong adhesion and durability, excellent compatibility, and easy manufacture of SCFG make it a promising hemostatic adhesive for clinical applications.
Collapse
Affiliation(s)
- Lisha Yu
- Department of Hepatobiliary and Pancreatic SurgeryThe Second Affiliated HospitalSchool of MedicineZhejiang UniversityHangzhouZhejiang310009China
- Key Laboratory of Precision Diagnosis and Treatment for Hepatobiliary and Pancreatic Tumor of Zhejiang ProvinceHangzhouZhejiang310009China
| | - Zhaodi Liu
- Department of Hepatobiliary and Pancreatic SurgeryThe Second Affiliated HospitalSchool of MedicineZhejiang UniversityHangzhouZhejiang310009China
- Key Laboratory of Precision Diagnosis and Treatment for Hepatobiliary and Pancreatic Tumor of Zhejiang ProvinceHangzhouZhejiang310009China
- Research Center of Diagnosis and Treatment Technology for Hepatocellular Carcinoma of Zhejiang ProvinceHangzhouZhejiang310009China
- National Innovation Center for Fundamental Research on Cancer MedicineHangzhouZhejiang310009China
- Cancer CenterZhejiang UniversityHangzhouZhejiang310058China
- ZJU‐Pujian Research & Development Center of Medical Artificial Intelligence for Hepatobiliary and Pancreatic DiseaseHangzhouZhejiang310058China
| | - Zongrui Tong
- Department of Hepatobiliary and Pancreatic SurgeryThe Second Affiliated HospitalSchool of MedicineZhejiang UniversityHangzhouZhejiang310009China
- Key Laboratory of Precision Diagnosis and Treatment for Hepatobiliary and Pancreatic Tumor of Zhejiang ProvinceHangzhouZhejiang310009China
| | - Yihang Ding
- MOE Key Laboratory of Macromolecular Synthesis and FunctionalizationDepartment of Polymer Science and EngineeringZhejiang UniversityHangzhouZhejiang310027China
| | - Zhefeng Qian
- Department of Hepatobiliary and Pancreatic SurgeryThe Second Affiliated HospitalSchool of MedicineZhejiang UniversityHangzhouZhejiang310009China
- Research Center of Diagnosis and Treatment Technology for Hepatocellular Carcinoma of Zhejiang ProvinceHangzhouZhejiang310009China
- MOE Key Laboratory of Macromolecular Synthesis and FunctionalizationDepartment of Polymer Science and EngineeringZhejiang UniversityHangzhouZhejiang310027China
| | - Weilin Wang
- Department of Hepatobiliary and Pancreatic SurgeryThe Second Affiliated HospitalSchool of MedicineZhejiang UniversityHangzhouZhejiang310009China
- Key Laboratory of Precision Diagnosis and Treatment for Hepatobiliary and Pancreatic Tumor of Zhejiang ProvinceHangzhouZhejiang310009China
- Research Center of Diagnosis and Treatment Technology for Hepatocellular Carcinoma of Zhejiang ProvinceHangzhouZhejiang310009China
- National Innovation Center for Fundamental Research on Cancer MedicineHangzhouZhejiang310009China
- Cancer CenterZhejiang UniversityHangzhouZhejiang310058China
- ZJU‐Pujian Research & Development Center of Medical Artificial Intelligence for Hepatobiliary and Pancreatic DiseaseHangzhouZhejiang310058China
| | - Zhengwei Mao
- Department of Hepatobiliary and Pancreatic SurgeryThe Second Affiliated HospitalSchool of MedicineZhejiang UniversityHangzhouZhejiang310009China
- Research Center of Diagnosis and Treatment Technology for Hepatocellular Carcinoma of Zhejiang ProvinceHangzhouZhejiang310009China
- MOE Key Laboratory of Macromolecular Synthesis and FunctionalizationDepartment of Polymer Science and EngineeringZhejiang UniversityHangzhouZhejiang310027China
| | - Yuan Ding
- Department of Hepatobiliary and Pancreatic SurgeryThe Second Affiliated HospitalSchool of MedicineZhejiang UniversityHangzhouZhejiang310009China
- Key Laboratory of Precision Diagnosis and Treatment for Hepatobiliary and Pancreatic Tumor of Zhejiang ProvinceHangzhouZhejiang310009China
- Research Center of Diagnosis and Treatment Technology for Hepatocellular Carcinoma of Zhejiang ProvinceHangzhouZhejiang310009China
- National Innovation Center for Fundamental Research on Cancer MedicineHangzhouZhejiang310009China
- Cancer CenterZhejiang UniversityHangzhouZhejiang310058China
- ZJU‐Pujian Research & Development Center of Medical Artificial Intelligence for Hepatobiliary and Pancreatic DiseaseHangzhouZhejiang310058China
| |
Collapse
|
|
9
|
Petrovskii VS, Zholudev SI, Potemkin II. Linear and ring polypeptides complexed with oppositely charged surfactants: the cohesion of the complexes as revealed in atomistic simulations. SOFT MATTER 2024; 20:388-396. [PMID: 38100081 DOI: 10.1039/d3sm01247f] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 01/04/2024]
Abstract
The use of linear supercharged unfolded polypeptides (SUPs) and oppositely charged surfactants in aqueous solution has demonstrated impressive adhesive properties. These substances possess biocompatibility, biodegradability and other necessary properties for practical application as a biomedical glue in wound repair. The success of these substances, coupled with limited knowledge about such systems, provides hope for enhancing the performance of the final product. One potential approach involves altering the topology of the polypeptide chain. In this article, we conduct a comparative analysis to examine the behavior of the ring and linear chains of a polypeptide in aqueous solution. This analysis utilizes full-atomic computer modeling to monitor the properties of the chains. We investigate the temperature dependence of the shape and size of individual polypeptides in the solution, as well as the formation of complexes via mixing the polypeptide chains with oppositely charged sodium dodecylbenzene sulfonate (SDBS) surfactant molecules in a stoichiometric ratio. Additionally, we explore the cohesive properties of the resulting complex through power experiments involving the extraction of single polypeptide chains out of the SUP-SDBS complexes.
Collapse
Affiliation(s)
- Vladislav S Petrovskii
- Physics Department, Lomonosov Moscow State University, Moscow 119991, Russian Federation.
- N. N. Semenov Federal Research Center for Chemical Physics, Russian Academy of Sciences, Moscow 119991, Russian Federation
| | - Stepan I Zholudev
- Physics Department, Lomonosov Moscow State University, Moscow 119991, Russian Federation.
| | - Igor I Potemkin
- Physics Department, Lomonosov Moscow State University, Moscow 119991, Russian Federation.
| |
Collapse
|
|
10
|
Guo Z, Xiong Y, Zhang S, Yuan T, Xia J, Wei R, Chen L, Sun W. Naturally derived highly resilient and adhesive hydrogels with application as surgical adhesive. Int J Biol Macromol 2023; 253:127192. [PMID: 37793510 DOI: 10.1016/j.ijbiomac.2023.127192] [Citation(s) in RCA: 4] [Impact Index Per Article: 2.0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 06/05/2023] [Revised: 09/29/2023] [Accepted: 09/29/2023] [Indexed: 10/06/2023]
Abstract
The inadequacy of conventional surgical techniques for wound closure and repair in soft and resilient tissues may lead to poor healing outcomes such as local tissue fibrosis and contracture. Therefore, the development of adhesive and resilient hydrogels that can adhere firmly to irregular and dynamic wound interfaces and provide a "tension-free proximity" environment for tissue regeneration has become extremely important. Herein, we describe an integrated modeling-experiment-application strategy for engineering a promising hydrogel-based bioadhesive based on recombinant human collagen (RHC) and catechol-modified hyaluronic acid (HA-Cat). Molecular modeling and simulations were used to verify and explore the hypothesis that RHC and HA-Cat can form an assembly complex through physical interactions. The complex was synergistically crosslinked via a catechol/o-quinone coupling reaction and a carbodiimide coupling reactions, resulting in superior hydrogels with strong adhesion and resilience properties. The application of this bioadhesive to tissue adhesion and wound sealing in vivo was successfully demonstrated, with an optimum collagen index, epidermal thickness, and lowest scar width. Furthermore, subcutaneous implantation demonstrated that the bioadhesive exhibited good biocompatibility and degradability. This newly developed hydrogel may be a highly promising surgical adhesive for medical applications, including wound closure and repair.
Collapse
Affiliation(s)
- Zhongwei Guo
- School of Mechanics and Safety Engineering, Zhengzhou University, Zhengzhou 450001, China
| | - Yahui Xiong
- Department of Burn, Wound Repair & Reconstruction, Laboratory of General Surgery, The First Affiliated Hospital, Sun Yat-Sen University, Guangzhou 510080, China; Institute of Precision Medicine, The First Affiliated Hospital, Sun Yat-Sen University, Guangzhou 510080, China
| | - Shiqiang Zhang
- School of Mechanics and Safety Engineering, Zhengzhou University, Zhengzhou 450001, China
| | - Tianying Yuan
- Department of Mechanical Engineering and Mechanics, Tsinghua University, Beijing 100084, China
| | - Jingjing Xia
- Department of Mechanical Engineering and Mechanics, Tsinghua University, Beijing 100084, China.
| | - Ronghan Wei
- School of Mechanics and Safety Engineering, Zhengzhou University, Zhengzhou 450001, China.
| | - Lei Chen
- Department of Burn, Wound Repair & Reconstruction, Laboratory of General Surgery, The First Affiliated Hospital, Sun Yat-Sen University, Guangzhou 510080, China; Institute of Precision Medicine, The First Affiliated Hospital, Sun Yat-Sen University, Guangzhou 510080, China.
| | - Wei Sun
- Department of Mechanical Engineering and Mechanics, Tsinghua University, Beijing 100084, China; Department of Mechanical Engineering, Drexel University, Philadelphia, PA 19104, United States.
| |
Collapse
|
|
11
|
Chen X, Yan G, Chen M, Yang P, Xu B. Alkylated chitosan-attapulgite composite sponge for rapid hemostasis. BIOMATERIALS ADVANCES 2023; 153:213569. [PMID: 37531822 DOI: 10.1016/j.bioadv.2023.213569] [Citation(s) in RCA: 12] [Impact Index Per Article: 6.0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 03/25/2023] [Revised: 06/30/2023] [Accepted: 07/23/2023] [Indexed: 08/04/2023]
Abstract
This study reported the development of a composite sponge (ACATS) based on alkylated chitosan (AC) and attapulgite (AT) for rapid hemostasis. The well-designed ACATS, with an optimal AC N-alkylation of 5.9 % and an optimal AC/AT mass ratio of 3:1, exhibited a hierarchical porous structure with a favorable biocompatibility. The ACATS can effectively and rapidly stop the uncontrolled bleeding in 235 ± 64 s with a total blood loss of 8.4 ± 4.0 g in comparison with those of Celox as a positive control (602 ± 101 s and 22.3 ± 2.4 g, respectively) using rabbit carotid artery injury model in vivo. ACATS could rapidly interact with blood and its components, including platelets (PLs), red blood cells (RBCs), and coagulation factors, resulting in these blood components rapidly accumulation and the following thrombus formation and coagulation factors activation.
Collapse
Affiliation(s)
- Xue Chen
- Department of Marine Biological Science & Technology, College of Ocean and Earth Sciences, Xiamen University, Xiamen 361102, China; State-Province Joint Engineering Laboratory of Marine Bioproducts and Technology, Xiamen University, Xiamen 361102, China
| | - Guoliang Yan
- Basic Medical Department of School of Medicine, Xiamen University, Xiamen 361102, China
| | - Ming Chen
- Department of Marine Biological Science & Technology, College of Ocean and Earth Sciences, Xiamen University, Xiamen 361102, China; State-Province Joint Engineering Laboratory of Marine Bioproducts and Technology, Xiamen University, Xiamen 361102, China; Shenzhen Research Institute of Xiamen University, Shenzhen 518000, China; Pingtan Research Institute of Xiamen University, Pingtan 350400, China.
| | - Ping Yang
- Department of Marine Biological Science & Technology, College of Ocean and Earth Sciences, Xiamen University, Xiamen 361102, China; State-Province Joint Engineering Laboratory of Marine Bioproducts and Technology, Xiamen University, Xiamen 361102, China
| | - Bolin Xu
- Department of Marine Biological Science & Technology, College of Ocean and Earth Sciences, Xiamen University, Xiamen 361102, China; State-Province Joint Engineering Laboratory of Marine Bioproducts and Technology, Xiamen University, Xiamen 361102, China
| |
Collapse
|
|
12
|
Zhao K, Li B, Sun Y, Jia B, Chen J, Cheng W, Zhao L, Li J, Wang F, Su J, Sun J, Han B, Liu Y, Zhang H, Liu K. Engineered Bicomponent Adhesives with Instantaneous and Superior Adhesion Performance for Wound Sealing and Healing Applications. ADVANCED FUNCTIONAL MATERIALS 2023; 33. [DOI: 10.1002/adfm.202303509] [Citation(s) in RCA: 5] [Impact Index Per Article: 2.5] [Reference Citation Analysis] [Abstract] [Track Full Text] [Subscribe] [Scholar Register] [Received: 03/29/2023] [Indexed: 10/05/2024]
Abstract
AbstractSurgical adhesives are playing an important role in wound repair and emergency hemostasis in clinical treatment. However, the development of strong bioglue with rapid in situ adhesion, durable adhesiveness, and flexibility in dynamic and moist physiological environments is still challenging. Herein, a new type of biosynthetic protein bioadhesives with superior adhesion performance is reported by developing a protein aldimine condensation strategy. Lysine‐rich recombinant proteins are designed and massively biosynthesized to instantaneously react with aldehyde cross‐linkers to realize in situ strong adhesion. The obtained bioadhesives show an ultra‐high adhesion strength of ≈101.6 kPa on porcine skin, outperforming extant clinical bioglues. In addition, they possess super biocompatibility, flexibility, biodegradability, and compliance with the tissues. Owing to the strong and instantaneous adhesion properties, the bioadhesives are qualified for dynamic wound closure, facilitating wound repair, and noncompressible hemorrhage. Importantly, they can be industrially encapsulated into custom‐made cartridge delivery tubes at low cost for clinical use. Therefore, biosynthetic bioadhesives have great potential for biological applications and are capable of scaling up to the industrial level for clinical transformation, which will be a successful paradigm for reforming existing clinical products.
Collapse
Affiliation(s)
- Kelu Zhao
- State Key Laboratory of Rare Earth Resource Utilization Changchun Institute of Applied Chemistry Chinese Academy of Sciences Changchun 130022 China
- University of Science and Technology of China Hefei 230026 China
| | - Bo Li
- Engineering Research Center of Advanced Rare Earth Materials Ministry of Education Department of Chemistry Tsinghua University Beijing 100084 China
| | - Yao Sun
- Engineering Research Center of Advanced Rare Earth Materials Ministry of Education Department of Chemistry Tsinghua University Beijing 100084 China
- Xiangfu Laboratory Jiaxing Zhejiang 314102 China
| | - Bo Jia
- State Key Laboratory of Rare Earth Resource Utilization Changchun Institute of Applied Chemistry Chinese Academy of Sciences Changchun 130022 China
| | - Jing Chen
- State Key Laboratory of Rare Earth Resource Utilization Changchun Institute of Applied Chemistry Chinese Academy of Sciences Changchun 130022 China
| | - Wenhao Cheng
- State Key Laboratory of Rare Earth Resource Utilization Changchun Institute of Applied Chemistry Chinese Academy of Sciences Changchun 130022 China
- University of Science and Technology of China Hefei 230026 China
| | - Lai Zhao
- State Key Laboratory of Rare Earth Resource Utilization Changchun Institute of Applied Chemistry Chinese Academy of Sciences Changchun 130022 China
| | - Jingjing Li
- State Key Laboratory of Rare Earth Resource Utilization Changchun Institute of Applied Chemistry Chinese Academy of Sciences Changchun 130022 China
| | - Fan Wang
- State Key Laboratory of Rare Earth Resource Utilization Changchun Institute of Applied Chemistry Chinese Academy of Sciences Changchun 130022 China
| | - Juanjuan Su
- College of Materials Science and Opto‐Electronic Technology University of Chinese Academy of Sciences Beijing 100049 China
| | - Jing Sun
- School of Chemistry and Molecular Engineering Shanghai Engineering Research Center of Molecular Therapeutics and New Drug Development East China Normal University Shanghai 200062 China
| | - Bing Han
- Department of Orthodontics Cranial‐Facial Growth, and Development Center Peking University School and Hospital of Stomatology and National Center of Stomatology and National Clinical Research Center for Oral Diseases and National Engineering Laboratory for Digital and Material Technology of Stomatology 22 Zhongguancun South Avenue, Haidian District Beijing 100081 China
| | - Yawei Liu
- State Key Laboratory of Rare Earth Resource Utilization Changchun Institute of Applied Chemistry Chinese Academy of Sciences Changchun 130022 China
| | - Hongjie Zhang
- State Key Laboratory of Rare Earth Resource Utilization Changchun Institute of Applied Chemistry Chinese Academy of Sciences Changchun 130022 China
- University of Science and Technology of China Hefei 230026 China
- Engineering Research Center of Advanced Rare Earth Materials Ministry of Education Department of Chemistry Tsinghua University Beijing 100084 China
- Xiangfu Laboratory Jiaxing Zhejiang 314102 China
| | - Kai Liu
- State Key Laboratory of Rare Earth Resource Utilization Changchun Institute of Applied Chemistry Chinese Academy of Sciences Changchun 130022 China
- University of Science and Technology of China Hefei 230026 China
- Engineering Research Center of Advanced Rare Earth Materials Ministry of Education Department of Chemistry Tsinghua University Beijing 100084 China
- Xiangfu Laboratory Jiaxing Zhejiang 314102 China
| |
Collapse
|
|
13
|
Lian X, Liao S, Han W, Song C, Wang Y. Stabilizing Liquid in Precise Nonequilibrium Shapes via Fast Interfacial Polymerization. SMALL (WEINHEIM AN DER BERGSTRASSE, GERMANY) 2023; 19:e2301039. [PMID: 37069770 DOI: 10.1002/smll.202301039] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Grants] [Track Full Text] [Subscribe] [Scholar Register] [Received: 02/06/2023] [Revised: 03/16/2023] [Indexed: 06/19/2023]
Abstract
Due to the minimization of interface area caused by surface tension, the stabilization of liquid in complex and precise nonequilibrium shapes is challenging. In this work, a simple, surfactant-free, and covalent strategy to stabilize liquid in precise nonequilibrium shapes via fast interfacial polymerization (FIP) of highly reactive n-butyl cyanoacrylate (BCA) monomer triggered by water-soluble nucleophiles is described. Full interfacial coverage can be achieved instantly, and the resultant polyBCA film anchored at the interface can support the unequal interface stress, which allows the production of non-spherical droplets with complex shapes. Notably, the formulation of internal aqueous phase is nearly unaffected since no specific additive is required. Moreover, considering the excellent biocompatibility of BCA and polyBCA, the produced droplets can be used as micro-bioreactor for enzyme catalysis and even bacterial culture, which well mimic the morphology of cells and bacteria to achieve the biochemical reaction in non-spherical droplets. The present work not only opens a new sight for the stabilization of liquid in nonequilibrium shapes, but may also promote the development of synthetic biology based on non-spherical droplets, and tremendous potential applications are anticipated.
Collapse
Affiliation(s)
- Xiaodong Lian
- Key Laboratory of Advanced Light Conversion Materials and Biophotonics, Department of Chemistry, Renmin University of China, Beijing, 100872, China
| | - Shenglong Liao
- Key Laboratory of Advanced Light Conversion Materials and Biophotonics, Department of Chemistry, Renmin University of China, Beijing, 100872, China
| | - Wenwen Han
- Key Laboratory of Advanced Light Conversion Materials and Biophotonics, Department of Chemistry, Renmin University of China, Beijing, 100872, China
| | - Chenhao Song
- Key Laboratory of Advanced Light Conversion Materials and Biophotonics, Department of Chemistry, Renmin University of China, Beijing, 100872, China
| | - Yapei Wang
- Key Laboratory of Advanced Light Conversion Materials and Biophotonics, Department of Chemistry, Renmin University of China, Beijing, 100872, China
| |
Collapse
|
|
14
|
Bingol HB, Bender JC, Opsteen JA, Leeuwenburgh SC. Bone adhesive materials: From bench to bedside. Mater Today Bio 2023; 19:100599. [PMID: 37063249 PMCID: PMC10102013 DOI: 10.1016/j.mtbio.2023.100599] [Citation(s) in RCA: 6] [Impact Index Per Article: 3.0] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 12/27/2022] [Revised: 02/24/2023] [Accepted: 03/01/2023] [Indexed: 03/06/2023] Open
Abstract
Biodegradable bone adhesives represent a highly sought-after type of biomaterial which would enable replacement of traditional metallic devices for fixation of bone. However, these biomaterials should fulfil an extremely large number of requirements. As a consequence, bone-adhesive biomaterials which meet all of these requirements are not yet commercially available. Therefore, this comprehensive review provides an extensive overview of the development of bone adhesives from a translational perspective. First, the definition, classification, and chemistry of various types of bone adhesives are highlighted to provide a detailed overview of this emerging class of biomaterials. In this review we particularly focused studies which describe the use of materials that are capable of gluing two pieces of bone together within a time frame of minutes to days. Second, this review critically reflects on i) the experimental conditions of commonly employed adhesion tests to assess bone adhesion and ii) the current state-of-the-art regarding their preclinical and clinical applicability.
Collapse
Affiliation(s)
- Hatice B. Bingol
- Department of Dentistry-Regenerative Biomaterials, Radboud Institute for Molecular Life Sciences, Radboud University Medical Center, Nijmegen, the Netherlands
- GATT Technologies BV, Nijmegen, the Netherlands
| | | | | | - Sander C.G. Leeuwenburgh
- Department of Dentistry-Regenerative Biomaterials, Radboud Institute for Molecular Life Sciences, Radboud University Medical Center, Nijmegen, the Netherlands
- Corresponding author.
| |
Collapse
|
|
15
|
Guo Y, Cheng N, Sun H, Hou J, Zhang Y, Wang D, Zhang W, Chen Z. Advances in the development and optimization strategies of the hemostatic biomaterials. Front Bioeng Biotechnol 2023; 10:1062676. [PMID: 36714615 PMCID: PMC9873964 DOI: 10.3389/fbioe.2022.1062676] [Citation(s) in RCA: 14] [Impact Index Per Article: 7.0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 10/06/2022] [Accepted: 12/29/2022] [Indexed: 01/12/2023] Open
Abstract
Most injuries are accompanied by acute bleeding. Hemostasis is necessary to relieve pain and reduce mortality in these accidents. In recent years, the traditional hemostatic materials, including inorganic, protein-based, polysaccharide-based and synthetic materials have been widely used in the clinic. The most prominent of these are biodegradable collagen sponges (Helistat®, United States), gelatin sponges (Ethicon®, SURGIFOAM®, United States), chitosan (AllaQuixTM, ChitoSAMTM, United States), cellulose (Tabotamp®, SURGICEL®, United States), and the newly investigated extracellular matrix gels, etc. Although these materials have excellent hemostatic properties, they also have their advantages and disadvantages. In this review, the performance characteristics, hemostatic effects, applications and hemostatic mechanisms of various biomaterials mentioned above are presented, followed by several strategies to improve hemostasis, including modification of single materials, blending of multiple materials, design of self-assembled peptides and their hybrid materials. Finally, the exploration of more novel hemostatic biomaterials and relative coagulation mechanisms will be essential for future research on hemostatic methods.
Collapse
Affiliation(s)
- Yayuan Guo
- Faculty of Life Science, Northwest University, Xi’an, Shaanxi Province, China
| | - Nanqiong Cheng
- Faculty of Life Science, Northwest University, Xi’an, Shaanxi Province, China
| | - Hongxiao Sun
- Faculty of Life Science, Northwest University, Xi’an, Shaanxi Province, China
| | - Jianing Hou
- Faculty of Life Science, Northwest University, Xi’an, Shaanxi Province, China
| | - Yuchen Zhang
- Faculty of Life Science, Northwest University, Xi’an, Shaanxi Province, China
| | - Du Wang
- Faculty of Life Science, Northwest University, Xi’an, Shaanxi Province, China
| | - Wei Zhang
- Faculty of Life Science, Northwest University, Xi’an, Shaanxi Province, China,School of Medicine, Northwest University, Xi’an, Shaanxi Province, China
| | - Zhuoyue Chen
- Faculty of Life Science, Northwest University, Xi’an, Shaanxi Province, China,School of Medicine, Northwest University, Xi’an, Shaanxi Province, China,*Correspondence: Zhuoyue Chen,
| |
Collapse
|
|
16
|
Narsingyani RG, Patel SM, Sood RA, Bhimani KK, Makwana SV, Savani RR. Attached Oral Mucosal Wound Closure using Blue Glue - A Prospective Clinical Study. Ann Maxillofac Surg 2023; 13:31-36. [PMID: 37711540 PMCID: PMC10499272 DOI: 10.4103/ams.ams_2_23] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 01/02/2023] [Revised: 04/28/2023] [Accepted: 06/02/2023] [Indexed: 09/16/2023] Open
Abstract
Introduction Traditionally, sutures have been widely used to close intraoral wounds. Various alternatives have been developed amongst which newer tissue adhesives such as N-butyl-2-cyanoacrylate having accepted clinical properties are gaining popularity. The primary purpose of the present study is to evaluate the efficacy of N-butyl-2-cyanoacrylate for intraoral attached mucosal wound closure following alveoloplasty. Materials and Methods In this prospective split-mouth study, 25 patients requiring alveoloplasty in either maxillary or mandibular arch bilaterally with a total of 50 sites were divided into two equal groups, namely silk suture (Group 1) and cyanoacrylate (Group 2). Each patient was evaluated on the first, third, seventh, 14th and 21st post-operative days. Parameters evaluated were time taken to close incision and to achieve haemostasis, pain, oedema, post-operative wound healing, patient discomfort and possible complications. Results Time taken to close incision, time taken to achieve haemostasis, post-operative pain and oedema were found to be less in Group 2. Wound healing too was found to be better in Group 2. There was one case of adhesive peel off on the first post-operative day which was managed by reapplying the tissue adhesive. The incidence of wound dehiscence and wound infection was observed more in Group 1 on the third post-operative day. Suture breakage (16%) and adhesive dislodgement (8%) were reported on the seventh post-operative day. Discussion The use of cyanoacrylates' inherent benefits, such as improved wound seal off in attached mucosa, quick polymerisation and bacteriostatic characteristics can be beneficial when performing minor oral surgical procedures on elderly, young and mentally challenged patients.
Collapse
Affiliation(s)
- Roma Gopaldas Narsingyani
- Department of Oral and Maxillofacial Surgery, AMC Dental College and Hospital, Gujarat University, Ahmedabad, Gujarat, India
| | - Shital Mayank Patel
- Department of Oral and Maxillofacial Surgery, AMC Dental College and Hospital, Gujarat University, Ahmedabad, Gujarat, India
| | - Ramita Atul Sood
- Department of Oral and Maxillofacial Surgery, AMC Dental College and Hospital, Gujarat University, Ahmedabad, Gujarat, India
| | - Kruna K. Bhimani
- Department of Oral and Maxillofacial Surgery, AMC Dental College and Hospital, Gujarat University, Ahmedabad, Gujarat, India
| | - Sanjaykumar V. Makwana
- Department of Oral and Maxillofacial Surgery, AMC Dental College and Hospital, Gujarat University, Ahmedabad, Gujarat, India
| | - Rajan R. Savani
- Department of Oral and Maxillofacial Surgery, AMC Dental College and Hospital, Gujarat University, Ahmedabad, Gujarat, India
| |
Collapse
|
|
17
|
Phoa S, Chan KS, Lim SH, Oo AM, Shelat VG. Comparison of glue versus suture mesh fixation for primary open inguinal hernia mesh repair by Lichtenstein technique: a systematic review and meta-analysis. Hernia 2022; 26:1105-1120. [PMID: 35113292 DOI: 10.1007/s10029-022-02571-4] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.3] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 11/15/2021] [Accepted: 01/13/2022] [Indexed: 02/05/2023]
Abstract
BACKGROUND The use of glue as a mesh fixator in open Lichtenstein inguinal hernia repair (IHR) has gained popularity to reduce recurrence and postoperative complications. This meta-analysis aims to provide an up-to-date review to compare glue versus suture fixation in primary open Lichtenstein IHR. METHODS PubMed, Embase, The Cochrane Library, Web of Science, and Springer were systematically searched till June 2021 for randomized controlled trials (RCTs) comparing glue versus suture fixation in open Lichtenstein IHR. Primary outcomes were early (at 1 year) and late recurrence (5 years or more). Secondary outcomes were the length of operation, postoperative haematoma and seroma, and chronic pain at 1 year. RESULTS A total of 17 RCTs with 3150 hernias (glue n = 1582, suture n = 1568) were included. Only three studies reported late recurrence. Glue fixation was associated with shorter operative duration (MD - 4.17, 95% CI - 4.82, - 3.52; p < 0.001 and a lower incidence of haematoma formation (OR 0.51, 95% CI 0.32, 0.81; p = 0.004). There was no significant difference in postoperative seroma (OR 0.72, 95% CI 0.35, 1.49; p = 0.38), chronic pain after 1 year (OR 1.10, 95% CI 0.73, 1.65; p = 0.65), early recurrence (OR 1.11, 95% CI 0.45, 2.76; p = 0.81, I2 = 0%), and late recurrence (OR 1.23, 95% CI 0.59, 2.59; p = 0.59, I2 = 0%). CONCLUSION Early and late recurrence were comparable between glue and suture fixation in open Lichtenstein IHR patients. Glue fixation had shorter operating time and lower haematoma formation than suture fixation. Chronic pain and seroma formation were comparable. More RCTs should report long-term outcomes.
Collapse
Affiliation(s)
- Shaun Phoa
- Yong Loo Lin School of Medicine, National University of Singapore, Singapore, 117597, Singapore
| | - Kai Siang Chan
- Department of General Surgery, Tan Tock Seng Hospital, Singapore, 308433, Singapore.
| | - Sioh Huang Lim
- Yong Loo Lin School of Medicine, National University of Singapore, Singapore, 117597, Singapore
| | - Aung Myint Oo
- Yong Loo Lin School of Medicine, National University of Singapore, Singapore, 117597, Singapore
- Department of General Surgery, Tan Tock Seng Hospital, Singapore, 308433, Singapore
- Lee Kong Chian School of Medicine, Nanyang Technological University, Singapore, 308232, Singapore
| | - Vishal G Shelat
- Yong Loo Lin School of Medicine, National University of Singapore, Singapore, 117597, Singapore
- Department of General Surgery, Tan Tock Seng Hospital, Singapore, 308433, Singapore
- Lee Kong Chian School of Medicine, Nanyang Technological University, Singapore, 308232, Singapore
| |
Collapse
|
|
18
|
Medical Adhesives and Their Role in Laparoscopic Surgery—A Review of Literature. MATERIALS 2022; 15:ma15155215. [PMID: 35955150 PMCID: PMC9369661 DOI: 10.3390/ma15155215] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.3] [Reference Citation Analysis] [Abstract] [Track Full Text] [Download PDF] [Subscribe] [Scholar Register] [Received: 04/27/2022] [Revised: 06/15/2022] [Accepted: 07/20/2022] [Indexed: 01/27/2023]
Abstract
Laparoscopic surgery is undergoing rapid development. Replacing the traditional method of joining cut tissues with sutures or staples could greatly simplify and speed up laparoscopic procedures. This alternative could undoubtedly be adhesives. For decades, scientists have been working on a material to bond tissues together to create the best possible conditions for tissue regeneration. The results of research on tissue adhesives achieved over the past years show comparable treatment effects to traditional methods. Tissue adhesives are a good alternative to surgical sutures in wound closure. This article is a review of the most important groups of tissue adhesives including their properties and possible applications. Recent reports on the development of biological adhesives are also discussed.
Collapse
|
|
19
|
Szymanski L, Gołaszewska K, Wiatrowska A, Dropik M, Krakowiak P, Małkowska J, Matak D. Biocompatibility of novel albumin-aldehyde surgical adhesive. Sci Rep 2022; 12:12749. [PMID: 35882896 PMCID: PMC9325888 DOI: 10.1038/s41598-022-16853-5] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 05/11/2022] [Accepted: 07/18/2022] [Indexed: 12/02/2022] Open
Abstract
Many medical procedures could benefit from the use of tissue sealants which allow for reduced surgery time, limited blood loss, easier tissue handling, and fewer postoperative complications. The safety and biocompatibility of surgical sealants are of paramount importance therefore, the aim of this study is to investigate the biocompatibility of NE’X Glue Surgical Adhesive. Chemical characterization (VOC and elements), cytotoxicity (MEM elution), genotoxicity (AMES and MLA), endotoxin contamination, sensitization potential, intracutaneous reactivity, acute and subchronic systemic toxicity with implantation as well as pyrogenicity were evaluated to investigate the biocompatibility of the NE’X Glue Surgical Adhesive. Studies were conducted according to ISO 10993 standards. The biocompatibility requirements with accordance to ISO 10993-1 for NE'X Glue were met. In vitro studies showed that NE'X Glue surgical adhesive is non-cytotoxic and non-mutagenic. Also, in vivo studies demonstrated that NE'X Glue shows no signs of toxicity, has no pyrogenic potential, and is non-sensitizing and non-irritating. The chemical characterization showed that no compounds were identified above Analytical Evaluation Threshold (AET), and no elements with concentrations higher than element-specific PDE (µg/day) were detected. NE'X Glue Surgical Adhesive is a versatile and promising new surgical sealant with a wide range of potential applications and very good biocompatibility.
Collapse
Affiliation(s)
- Lukasz Szymanski
- Department of Molecular Biology, Institute of Genetics and Animal Biotechnology, Polish Academy of Science, Postępu 36A, 05-552, Magdalenka, Poland.,European Biomedical Institute, Nalkowskiej 5, 05-410, Jozefow, Poland
| | | | - Anna Wiatrowska
- European Biomedical Institute, Nalkowskiej 5, 05-410, Jozefow, Poland
| | - Monika Dropik
- European Biomedical Institute, Nalkowskiej 5, 05-410, Jozefow, Poland
| | | | - Justyna Małkowska
- European Biomedical Institute, Nalkowskiej 5, 05-410, Jozefow, Poland
| | - Damian Matak
- European Biomedical Institute, Nalkowskiej 5, 05-410, Jozefow, Poland.
| |
Collapse
|
|
20
|
Montazerian H, Davoodi E, Baidya A, Baghdasarian S, Sarikhani E, Meyer CE, Haghniaz R, Badv M, Annabi N, Khademhosseini A, Weiss PS. Engineered Hemostatic Biomaterials for Sealing Wounds. Chem Rev 2022; 122:12864-12903. [PMID: 35731958 DOI: 10.1021/acs.chemrev.1c01015] [Citation(s) in RCA: 118] [Impact Index Per Article: 39.3] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 12/12/2022]
Abstract
Hemostatic biomaterials show great promise in wound control for the treatment of uncontrolled bleeding associated with damaged tissues, traumatic wounds, and surgical incisions. A surge of interest has been directed at boosting hemostatic properties of bioactive materials via mechanisms triggering the coagulation cascade. A wide variety of biocompatible and biodegradable materials has been applied to the design of hemostatic platforms for rapid blood coagulation. Recent trends in the design of hemostatic agents emphasize chemical conjugation of charged moieties to biomacromolecules, physical incorporation of blood-coagulating agents in biomaterials systems, and superabsorbing materials in either dry (foams) or wet (hydrogel) states. In addition, tough bioadhesives are emerging for efficient and physical sealing of incisions. In this Review, we highlight the biomacromolecular design approaches adopted to develop hemostatic bioactive materials. We discuss the mechanistic pathways of hemostasis along with the current standard experimental procedures for characterization of the hemostasis efficacy. Finally, we discuss the potential for clinical translation of hemostatic technologies, future trends, and research opportunities for the development of next-generation surgical materials with hemostatic properties for wound management.
Collapse
Affiliation(s)
- Hossein Montazerian
- Department of Bioengineering, University of California, Los Angeles, 410 Westwood Plaza, Los Angeles, California 90095, United States.,California NanoSystems Institute, University of California, Los Angeles, Los Angeles, California 90095, United States.,Terasaki Institute for Biomedical Innovation, Los Angeles, California 90024, United States
| | - Elham Davoodi
- Department of Bioengineering, University of California, Los Angeles, 410 Westwood Plaza, Los Angeles, California 90095, United States.,California NanoSystems Institute, University of California, Los Angeles, Los Angeles, California 90095, United States.,Terasaki Institute for Biomedical Innovation, Los Angeles, California 90024, United States.,Multi-Scale Additive Manufacturing Lab, Mechanical and Mechatronics Engineering Department, University of Waterloo, 200 University Avenue West, Waterloo, Ontario N2L 3G1, Canada
| | - Avijit Baidya
- Department of Chemical and Biomolecular Engineering, University of California, Los Angeles, Los Angeles, California 90095, United States
| | - Sevana Baghdasarian
- Department of Chemical and Biomolecular Engineering, University of California, Los Angeles, Los Angeles, California 90095, United States
| | - Einollah Sarikhani
- Department of Bioengineering, University of California, Los Angeles, 410 Westwood Plaza, Los Angeles, California 90095, United States
| | - Claire Elsa Meyer
- Department of Chemistry and Biochemistry, University of California, Los Angeles, Los Angeles, California 90095, United States
| | - Reihaneh Haghniaz
- Terasaki Institute for Biomedical Innovation, Los Angeles, California 90024, United States
| | - Maryam Badv
- California NanoSystems Institute, University of California, Los Angeles, Los Angeles, California 90095, United States.,Department of Chemistry and Biochemistry, University of California, Los Angeles, Los Angeles, California 90095, United States.,Department of Biomedical Engineering, Schulich School of Engineering, University of Calgary, Calgary, Alberta T2N 1N4, Canada
| | - Nasim Annabi
- Department of Bioengineering, University of California, Los Angeles, 410 Westwood Plaza, Los Angeles, California 90095, United States.,Department of Chemical and Biomolecular Engineering, University of California, Los Angeles, Los Angeles, California 90095, United States
| | - Ali Khademhosseini
- Terasaki Institute for Biomedical Innovation, Los Angeles, California 90024, United States
| | - Paul S Weiss
- Department of Bioengineering, University of California, Los Angeles, 410 Westwood Plaza, Los Angeles, California 90095, United States.,California NanoSystems Institute, University of California, Los Angeles, Los Angeles, California 90095, United States.,Department of Chemistry and Biochemistry, University of California, Los Angeles, Los Angeles, California 90095, United States.,Department of Materials Science and Engineering, University of California, Los Angeles, Los Angeles, California 90095, United States
| |
Collapse
|
|
21
|
YILMAZ M, KAYAALTI-YÜKSEK S, KARADUMAN B. The Effects of Cyanoacrylate on Clinical Healing and Self-Reported Outcomes Following Free Gingival Graft Surgery: A Randomized Clinical Study. CLINICAL AND EXPERIMENTAL HEALTH SCIENCES 2022. [DOI: 10.33808/clinexphealthsci.1012775] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/22/2022]
Abstract
Objective: This study aims to reveal the effects of cyanoacrylate application at the recipient bed and the donor site in free gingival graft surgery on graft dimensions, clinical healing parameters and patient-based outcomes.
Methods: Free gingival graft operations were conducted on individuals who were randomly assigned to control or test groups. In the test group, the graft was stabilized and the donor site was coated with cyanoacrylate. In the control group, 6/0 polyvinylidene fluoride sutures were used for stabilization, while the donor site was left untreated. Variables including re-epithelization, post-operative complications, pain, bleeding and quality of life at recipient site, and color match, graft dimensions at donor site were assessed for up to 6 months.
Results: Twenty-three individuals completed the study. No differences were observed in any variable between groups except horizontal dimension loss of the graft, which was more abundant in the cyanoacrylate group at six months (p>0.05, p
Collapse
|
|
22
|
Ma X, Bian Q, Hu J, Gao J. Stem from nature: Bioinspired adhesive formulations for wound healing. J Control Release 2022; 345:292-305. [DOI: 10.1016/j.jconrel.2022.03.027] [Citation(s) in RCA: 2] [Impact Index Per Article: 0.7] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 01/29/2022] [Revised: 03/13/2022] [Accepted: 03/14/2022] [Indexed: 12/27/2022]
|
|
23
|
Akgunduz F, Sozutek A, Irkorucu O, Yaman A. The Adjunctive Effect of DuraSeal® vs. 2-Octyl-Cyanoacrylate on Delayed Repair of Gastric Perforation: An Experimental Study. J INVEST SURG 2022; 35:542-548. [PMID: 33645439 DOI: 10.1080/08941939.2021.1887414] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 10/22/2022]
Abstract
OBJECTIVE Delayed primary suture closure of gastric perforation is prone to dehiscence hence the repaired area should be buttressed to avoid re-operation. We aimed to investigate whether DuraSeal®(DS) has a potent adjunctive effect on delayed closure of gastric perforation comparing with 2-octly-cyanoacrylate(CYN) in an experimental model. METHODS Sixty rats were randomly divided into 6 groups. All subjected to gastric perforation, subsequently perforation areas were repaired by primary suturing, delayed repair was performed 12 h after surgery. According to DS or CYN application on anastomosis, the groups were classified as control(C), delayed control(CD), closure with CYN(CYN), delayed closure with CYN(D-CYN), closure with DS(DS), delayed closure with DS(D-DS).After euthanization on POD 7,anastomotic bursting pressure(ABP) were measured. Tissue samples were taken for histopathological examination and hydroxyproline(TH) assessment. RESULTS Delayed condition significantly reduced ABP and TH levels in CD group comparing with all groups(p < 0.01).Either CYN or DS application on delayed repaired area significantly raised the measure of ABP and TH up to the levels of C group(p < 0.05,comparing with CD).Microscopically,either CYN or DS application significantly improved tissue necrosis, submucosal bridging and collagen formation comparing with CD group(p < 0.012).There were no difference regarding ABP, TH and tissue healing between each CYN and DS groups. CONCLUSION DuraSeal® application on sutured gastric perforation area yielded a significant adjunctive effect both in normal and delayed conditions. However, DuraSeal® revealed no superior effect to CYN in both condition.Our results demonstrated that the clinical use of DuraSeal® can be considered for reinforcing the sutured line in patients undergoing delayed surgery for gastric perforation.
Collapse
Affiliation(s)
- Fatih Akgunduz
- General Surgery, Health Sciences University, Adana Training and Research Hospital, Adana, Turkey
| | - Alper Sozutek
- Gastroenterological Surgery, Health Sciences University, Adana Training and Research Hospital, Adana, Turkey
| | - Oktay Irkorucu
- General Surgery, Health Sciences University, Adana Training and Research Hospital, Adana, Turkey
| | - Abit Yaman
- General Surgery, Health Sciences University, Adana Training and Research Hospital, Adana, Turkey
| |
Collapse
|
|
24
|
Ghovvati M, Baghdasarian S, Baidya A, Dhal J, Annabi N. Engineering a highly elastic bioadhesive for sealing soft and dynamic tissues. J Biomed Mater Res B Appl Biomater 2022; 110:1511-1522. [PMID: 35148016 DOI: 10.1002/jbm.b.35012] [Citation(s) in RCA: 6] [Impact Index Per Article: 2.0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 11/23/2020] [Revised: 12/27/2021] [Accepted: 01/11/2022] [Indexed: 12/19/2022]
Abstract
Injured tissues often require immediate closure to restore the normal functionality of the organ. In most cases, injuries are associated with trauma or various physical surgeries where different adhesive hydrogel materials are applied to close the wounds. However, these materials are typically toxic, have low elasticity, and lack strong adhesion especially to the wet tissues. In this study, a stretchable composite hydrogel consisting of gelatin methacrylol catechol (GelMAC) with ferric ions, and poly(ethylene glycol) diacrylate (PEGDA) was developed. The engineered material could adhere to the wet tissue surfaces through the chemical conjugation of catechol and methacrylate groups to the gelatin backbone. Moreover, the incorporation of PEGDA enhanced the elasticity of the bioadhesives. Our results showed that the physical properties and adhesion of the hydrogels could be tuned by changing the ratio of GelMAC/PEGDA. In addition, the in vitro toxicity tests confirmed the biocompatibility of the engineered bioadhesives. Finally, using an ex vivo lung incision model, we showed the potential application of the developed bioadhesives for sealing elastic tissues.
Collapse
Affiliation(s)
- Mahsa Ghovvati
- Department of Chemical and Biomolecular Engineering, University of California - Los Angeles, Los Angeles, California, USA
| | - Sevana Baghdasarian
- Department of Chemical and Biomolecular Engineering, University of California - Los Angeles, Los Angeles, California, USA
| | - Avijit Baidya
- Department of Chemical and Biomolecular Engineering, University of California - Los Angeles, Los Angeles, California, USA
| | - Jharana Dhal
- Department of Chemical and Biomolecular Engineering, University of California - Los Angeles, Los Angeles, California, USA
| | - Nasim Annabi
- Department of Chemical and Biomolecular Engineering, University of California - Los Angeles, Los Angeles, California, USA
| |
Collapse
|
|
25
|
Zheng K, Gu Q, Zhou D, Zhou M, Zhang L. Recent progress in surgical adhesives for biomedical applications. SMART MATERIALS IN MEDICINE 2022; 3:41-65. [DOI: 10.1016/j.smaim.2021.11.004] [Citation(s) in RCA: 28] [Impact Index Per Article: 9.3] [Reference Citation Analysis] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 01/06/2025]
|
|
26
|
Ghimire S, Sarkar P, Rigby K, Maan A, Mukherjee S, Crawford KE, Mukhopadhyay K. Polymeric Materials for Hemostatic Wound Healing. Pharmaceutics 2021; 13:2127. [PMID: 34959408 PMCID: PMC8708336 DOI: 10.3390/pharmaceutics13122127] [Citation(s) in RCA: 32] [Impact Index Per Article: 8.0] [Reference Citation Analysis] [Abstract] [Key Words] [Grants] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 11/05/2021] [Revised: 11/26/2021] [Accepted: 12/01/2021] [Indexed: 02/04/2023] Open
Abstract
Hemorrhage is one of the greatest threats to life on the battlefield, accounting for 50% of total deaths. Nearly 86% of combat deaths occur within the first 30 min after wounding. While external wound injuries can be treated mostly using visual inspection, abdominal or internal hemorrhages are more challenging to treat with regular hemostatic dressings because of deep wounds and points of injury that cannot be located properly. The need to treat trauma wounds from limbs, abdomen, liver, stomach, colon, spleen, arterial, venous, and/or parenchymal hemorrhage accompanied by severe bleeding requires an immediate solution that the first responders can apply to reduce rapid exsanguinations from external wounds, including in military operations. This necessitates the development of a unique, easy-to-use, FDA-approved hemostatic treatment that can deliver the agent in less than 30 s and stop bleeding within the first 1 to 2 min at the point of injury without application of manual pressure on the wounded area.
Collapse
Affiliation(s)
- Suvash Ghimire
- Department of Materials Science and Engineering, University of Central Florida, Orlando, FL 32816, USA; (S.G.); (P.S.); (K.R.); (A.M.); (S.M.)
| | - Pritha Sarkar
- Department of Materials Science and Engineering, University of Central Florida, Orlando, FL 32816, USA; (S.G.); (P.S.); (K.R.); (A.M.); (S.M.)
| | - Kasey Rigby
- Department of Materials Science and Engineering, University of Central Florida, Orlando, FL 32816, USA; (S.G.); (P.S.); (K.R.); (A.M.); (S.M.)
| | - Aditya Maan
- Department of Materials Science and Engineering, University of Central Florida, Orlando, FL 32816, USA; (S.G.); (P.S.); (K.R.); (A.M.); (S.M.)
- Department of Chemistry, University of Central Florida, Orlando, FL 32816, USA
| | - Santanu Mukherjee
- Department of Materials Science and Engineering, University of Central Florida, Orlando, FL 32816, USA; (S.G.); (P.S.); (K.R.); (A.M.); (S.M.)
| | - Kaitlyn E. Crawford
- Department of Materials Science and Engineering, University of Central Florida, Orlando, FL 32816, USA; (S.G.); (P.S.); (K.R.); (A.M.); (S.M.)
- Department of Chemistry, University of Central Florida, Orlando, FL 32816, USA
- NanoScience Technology Center, University of Central Florida, Orlando, FL 32816, USA
- Biionix Cluster, University of Central Florida, Orlando, FL 32816, USA
| | - Kausik Mukhopadhyay
- Department of Materials Science and Engineering, University of Central Florida, Orlando, FL 32816, USA; (S.G.); (P.S.); (K.R.); (A.M.); (S.M.)
| |
Collapse
|
|
27
|
Bunkar SK, Verma K, Jhunjhunwala A, Singh A. A Randomized Controlled Clinical Trial of N-Butyl Cyanoacrylate Glue Fixation Versus Tacker Fixation of Mesh in Endoscopic Totally Extraperitoneal Hernia Repair. Indian J Surg 2021. [DOI: 10.1007/s12262-021-03118-0] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.3] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/27/2022] Open
|
|
28
|
Ma C, Sun J, Li B, Feng Y, Sun Y, Xiang L, Wu B, Xiao L, Liu B, Petrovskii VS, Bin Liu, Zhang J, Wang Z, Li H, Zhang L, Li J, Wang F, Gӧstl R, Potemkin II, Chen D, Zeng H, Zhang H, Liu K, Herrmann A. Ultra-strong bio-glue from genetically engineered polypeptides. Nat Commun 2021; 12:3613. [PMID: 34127656 PMCID: PMC8203747 DOI: 10.1038/s41467-021-23117-9] [Citation(s) in RCA: 101] [Impact Index Per Article: 25.3] [Reference Citation Analysis] [Abstract] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 11/09/2020] [Accepted: 03/19/2021] [Indexed: 12/23/2022] Open
Abstract
The development of biomedical glues is an important, yet challenging task as seemingly mutually exclusive properties need to be combined in one material, i.e. strong adhesion and adaption to remodeling processes in healing tissue. Here, we report a biocompatible and biodegradable protein-based adhesive with high adhesion strengths. The maximum strength reaches 16.5 ± 2.2 MPa on hard substrates, which is comparable to that of commercial cyanoacrylate superglue and higher than other protein-based adhesives by at least one order of magnitude. Moreover, the strong adhesion on soft tissues qualifies the adhesive as biomedical glue outperforming some commercial products. Robust mechanical properties are realized without covalent bond formation during the adhesion process. A complex consisting of cationic supercharged polypeptides and anionic aromatic surfactants with lysine to surfactant molar ratio of 1:0.9 is driven by multiple supramolecular interactions enabling such strong adhesion. We demonstrate the glue's robust performance in vitro and in vivo for cosmetic and hemostasis applications and accelerated wound healing by comparison to surgical wound closures.
Collapse
Affiliation(s)
- Chao Ma
- Department of Chemistry, Tsinghua University, Beijing, China.,Zernike Institute for Advanced Materials, University of Groningen, Groningen, The Netherlands.,School of Engineering and Applied Sciences, Harvard University, Cambridge, MA, USA
| | - Jing Sun
- School of Engineering and Applied Sciences, Harvard University, Cambridge, MA, USA.,State Key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, China
| | - Bo Li
- State Key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, China
| | - Yang Feng
- State Key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, China
| | - Yao Sun
- State Key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, China
| | - Li Xiang
- Department of Chemical and Materials Engineering, University of Alberta, Edmonton, Alberta, Canada
| | - Baiheng Wu
- Institute of Process Equipment, College of energy engineering, Zhejiang University, Hangzhou, China
| | - Lingling Xiao
- State Key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, China
| | - Baimei Liu
- State Key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, China
| | - Vladislav S Petrovskii
- Physics Department, Lomonosov Moscow State University, Moscow, Russian Federation.,N. N. Semenov Institute of Chemical Physics, Russian Academy of Sciences, Moscow, Russian Federation
| | - Bin Liu
- State Key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, China
| | - Jinrui Zhang
- State Key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, China
| | - Zili Wang
- State Key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, China
| | - Hongyan Li
- Zernike Institute for Advanced Materials, University of Groningen, Groningen, The Netherlands.,DWI - Leibniz Institute for Interactive Materials, Aachen, Germany
| | - Lei Zhang
- State Key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, China
| | - Jingjing Li
- State Key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, China
| | - Fan Wang
- State Key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, China
| | - Robert Gӧstl
- DWI - Leibniz Institute for Interactive Materials, Aachen, Germany
| | - Igor I Potemkin
- Physics Department, Lomonosov Moscow State University, Moscow, Russian Federation.,DWI - Leibniz Institute for Interactive Materials, Aachen, Germany.,National Research South Ural State University, Chelyabinsk, Russian Federation
| | - Dong Chen
- Institute of Process Equipment, College of energy engineering, Zhejiang University, Hangzhou, China
| | - Hongbo Zeng
- Department of Chemical and Materials Engineering, University of Alberta, Edmonton, Alberta, Canada
| | - Hongjie Zhang
- Department of Chemistry, Tsinghua University, Beijing, China.,State Key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, China
| | - Kai Liu
- Department of Chemistry, Tsinghua University, Beijing, China. .,State Key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, China.
| | - Andreas Herrmann
- Zernike Institute for Advanced Materials, University of Groningen, Groningen, The Netherlands. .,DWI - Leibniz Institute for Interactive Materials, Aachen, Germany. .,Institute of Technical and Macromolecular Chemistry, RWTH Aachen University, Aachen, Germany.
| |
Collapse
|
|
29
|
Orak Y, Eroğlu E, Baylan FA, Yıldız S, Boran ÖF, Doganer A, Altun A. Effects of acupuncture on pain and levels of IL-17 and IL-23 in the treatment of non-thermal endovenous ablation: A randomized clinical trial. Vascular 2021; 30:532-541. [PMID: 33977797 DOI: 10.1177/17085381211013980] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/16/2022]
Abstract
OBJECTIVES Acupuncture is one of the oldest therapeutic interventions in the world for the treatment of pain, musculoskeletal diseases, and inflammation. This study aimed to investigate the effect of acupuncture on pain and IL-17 and IL-23 levels in the treatment of endovenous ablation. METHODS The study was a randomized controlled trial. Patients were divided into group C (Control, n = 35) and group A (Acupuncture, n = 35). Group A patients were treated with acupuncture 24 h preoperatively. Follow-up checkups were conducted intraoperatively, postoperatively, and on the third day. RESULTS There was no difference between men; there was a difference between women. Visual analog scale score was lower in group A at the intraoperative third and fifth minutes (0.00 vs. 1 and 0.00 vs. 0.5). Analgesic consumption was lower in group A at the end of third day (p = 0.024). Postoperative IL-17 levels were higher than preoperative levels in group A (23.58 vs. 19.33). Postoperative IL-23 levels were lower than preoperative levels in group A (13.66 vs. 29.51). Group C showed increased postoperative IL-23 levels (28.81 vs. 33.51). Preoperative IL-17 and postoperative IL-23 levels were lower in group A than in group C (19.33 vs. 27.69 and 13.66 vs. 33.51). Although no difference was observed between group A and group C in preoperative saphenous vein diameter, postoperative saphenous vein diameter was smaller in group A (p = 0.008). Saphenous vein diameter was smaller on day 3 in group A than in group C (p = 0.043). CONCLUSION Acupuncture is effective on acute pain and level of IL-23 in the treatment of endovenous ablation using cyanoacrylate.
Collapse
Affiliation(s)
- Yavuz Orak
- Department of Anesthesiology and Reanimation, Faculty of Medicine, Kahramanmaraş Sutçu Imam University, Kahramanmaraş, Turkey
| | - Erdinç Eroğlu
- Department of Cardiovascular Surgery, Faculty of Medicine, Kahramanmaraş Sutçu Imam University, Kahramanmaraş, Turkey
| | - Filiz Alkan Baylan
- Department of Biochemistry, Faculty of Medicine, Kahramanmaraş Sutçu Imam University, Kahramanmaraş, Turkey
| | - Sedat Yıldız
- Private Doctor's Office, Physical Medicine and Rehabilitation Department, Isparta, Turkey
| | - Ömer Faruk Boran
- Department of Anesthesiology and Reanimation, Faculty of Medicine, Kahramanmaraş Sutçu Imam University, Kahramanmaraş, Turkey
| | - Adem Doganer
- Department of Biostatistics and Medical Informatics, Faculty of Medicine, Kahramanmaraş Sutçu Imam University, Kahramanmaraş, Turkey
| | - Ahmet Altun
- Department of Medical Pharmacology, Faculty of Medicine, Sivas Cumhuriyet University, Sivas, Turkey
| |
Collapse
|
|
30
|
Tiralongo F, Distefano G, Palermo M, Granata A, Giurazza F, Vacirca F, Palmucci S, Venturini M, Basile A. Liquid and Solid Embolic Agents in Gonadal Veins. J Clin Med 2021; 10:1596. [PMID: 33918908 PMCID: PMC8069975 DOI: 10.3390/jcm10081596] [Citation(s) in RCA: 5] [Impact Index Per Article: 1.3] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 03/17/2021] [Revised: 03/29/2021] [Accepted: 04/07/2021] [Indexed: 12/05/2022] Open
Abstract
Male varicocele and pelvic congestion syndrome (PCS) are common pathologies with high predominance in young patients, having a high impact on the quality of life and infertility. Lately, the use of different endovascular embolization techniques, with various embolizing agents, shows good technical results and clinical outcomes. With the aim of presenting the "state of the art" of endovascular techniques for the treatment of male varicocele and PCS, and to discuss the performance of the different embolic agents proposed, we conducted an extensive analysis of the relevant literature and we reported and discussed the results of original studies and previous meta-analyses, providing an updated guide on this topic to clinicians and interventional radiologists. We have also underlined the technical aspects for the benefit of those who approach this type of interventional treatment. Our review suggests promising results in both the endovascular embolic treatment of male varicocele and PCS; for varicocele, a success rate of between 70% and 100% and a recurrence rate of up to 16% is reported, while for PCS it has been found that technical success is achieved in almost all cases of endovascular treatment, with a highly variable recurrence rate based on reports. Complications are overall rather rare and are represented by periprocedural pain, migration of embolic media and vascular perforations: severe adverse events have been reported very rarely.
Collapse
Affiliation(s)
- Francesco Tiralongo
- Radiology Unit I, Department of Medical Surgical Sciences and Advanced Technologies “GF Ingrassia”–University Hospital “Policlinico-San Marco”, University of Catania, Via Santa Sofia n° 78, 95123 Catania, Italy; (G.D.); (M.P.); (F.V.); (S.P.); (A.B.)
| | - Giulio Distefano
- Radiology Unit I, Department of Medical Surgical Sciences and Advanced Technologies “GF Ingrassia”–University Hospital “Policlinico-San Marco”, University of Catania, Via Santa Sofia n° 78, 95123 Catania, Italy; (G.D.); (M.P.); (F.V.); (S.P.); (A.B.)
| | - Monica Palermo
- Radiology Unit I, Department of Medical Surgical Sciences and Advanced Technologies “GF Ingrassia”–University Hospital “Policlinico-San Marco”, University of Catania, Via Santa Sofia n° 78, 95123 Catania, Italy; (G.D.); (M.P.); (F.V.); (S.P.); (A.B.)
| | - Antonio Granata
- Nephrology and Dialysis Unit, “Cannizzaro” Hospital, 95123 Catania, Italy;
| | - Francesco Giurazza
- Interventional Radiology Department, Cardarelli Hospital of Naples, 80131 Naples, Italy;
| | - Francesco Vacirca
- Radiology Unit I, Department of Medical Surgical Sciences and Advanced Technologies “GF Ingrassia”–University Hospital “Policlinico-San Marco”, University of Catania, Via Santa Sofia n° 78, 95123 Catania, Italy; (G.D.); (M.P.); (F.V.); (S.P.); (A.B.)
| | - Stefano Palmucci
- Radiology Unit I, Department of Medical Surgical Sciences and Advanced Technologies “GF Ingrassia”–University Hospital “Policlinico-San Marco”, University of Catania, Via Santa Sofia n° 78, 95123 Catania, Italy; (G.D.); (M.P.); (F.V.); (S.P.); (A.B.)
| | - Massimo Venturini
- Department of Diagnostic and Interventional Radiology, Circolo Hospital, Insubria University, 21100 Varese, Italy;
| | - Antonio Basile
- Radiology Unit I, Department of Medical Surgical Sciences and Advanced Technologies “GF Ingrassia”–University Hospital “Policlinico-San Marco”, University of Catania, Via Santa Sofia n° 78, 95123 Catania, Italy; (G.D.); (M.P.); (F.V.); (S.P.); (A.B.)
| |
Collapse
|
|
31
|
Al-Masri M, Alawneh F, Daoud F, Ebous A, Hamdan B, Al-Najjar H, Al-Masri R, Abufara M. Effectiveness of Cyanoacrylate in Reducing Seroma Formation in Breast Cancer Patients Post-Axillary Dissection: A Randomized Controlled Trial. Front Oncol 2021; 10:580861. [PMID: 33569343 PMCID: PMC7868378 DOI: 10.3389/fonc.2020.580861] [Citation(s) in RCA: 4] [Impact Index Per Article: 1.0] [Reference Citation Analysis] [Abstract] [Key Words] [Grants] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 07/07/2020] [Accepted: 11/27/2020] [Indexed: 11/25/2022] Open
Abstract
BACKGROUND/PURPOSE Seroma is a common complication after axillary dissection in women with node-positive breast cancer. We aim to determine the effect of Cyanoacrylate on reducing seroma formation in patients undergoing axillary dissection. This a randomized clinical trial. METHODS This is a single-center, randomized, single-blinded, and two-arm parallel study. Women with node-positive breast cancer eligible for axillary dissection were enrolled. Patients with a Body Mass Index (BMI) greater than 35 kg/m2, those who underwent immediate breast reconstruction, and/or received neoadjuvant chemotherapy were excluded. Patients were randomized in a 1:1 ratio, and were stratified according to their age, BMI, tumor size, and operation type. The primary endpoint was the total seroma volume (the total drained volume and the total aspirated volume after drain removal). Data presented as mean and range when applicable. RESULTS 111 patients were randomized (Cyanoacrylate 57; control 54). 105 patients were analyzed. Sixty-nine patients underwent breast conserving surgery, and 36 underwent modified radical mastectomy. There was no difference in the total seroma volume between the Cyanoacrylate vs. control arms (1,304 (60-4,950) vs. 1,446 (100-5,223) ml, p=0.458). Wound infection, flap necrosis, number of manual aspirates, and hematoma formation were not statistically different between the two groups. Time to drain removal was shorter in the Cyanoacrylate arm (11.04(3-23) vs. 13.84(3-37) days, p=0.015). The use of Cyanoacrylate was not cost effective ($586.93 (550-748) vs. $29.63 (0-198), p<0.001). Higher seroma volume was correlated with modified radical mastectomy, older age, and BMI more than 30 kg/m2. CONCLUSION Cyanoacrylate did not reduce seroma formation and its use was not cost effective. CLINICAL TRIAL REGISTRATION clinicaltrials.gov, identifier NCT02141373.
Collapse
Affiliation(s)
- Mahmoud Al-Masri
- Department of Surgery, King Hussein Cancer Center, Amman, Jordan
| | - Fade Alawneh
- Department of Surgery, King Hussein Cancer Center, Amman, Jordan
| | - Faiez Daoud
- Department of Surgery, King Hussein Cancer Center, Amman, Jordan
| | - Ali Ebous
- Department of Surgery, King Hussein Cancer Center, Amman, Jordan
| | - Basem Hamdan
- Department of Surgery, King Hussein Cancer Center, Amman, Jordan
| | - Hani Al-Najjar
- Department of Surgery, King Hussein Cancer Center, Amman, Jordan
| | - Rama Al-Masri
- School of Medicine, University of Jordan, Amman, Jordan
| | - Marwan Abufara
- Department of Surgery, King Hussein Cancer Center, Amman, Jordan
| |
Collapse
|
|
32
|
Gowda AH, Bu Y, Kudina O, Krishna KV, Bohara RA, Eglin D, Pandit A. Design of tunable gelatin-dopamine based bioadhesives. Int J Biol Macromol 2020; 164:1384-1391. [DOI: 10.1016/j.ijbiomac.2020.07.195] [Citation(s) in RCA: 27] [Impact Index Per Article: 5.4] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 04/23/2020] [Revised: 07/16/2020] [Accepted: 07/17/2020] [Indexed: 11/28/2022]
|
|
33
|
Li D, Chen J, Wang X, Zhang M, Li C, Zhou J. Recent Advances on Synthetic and Polysaccharide Adhesives for Biological Hemostatic Applications. Front Bioeng Biotechnol 2020; 8:926. [PMID: 32923431 PMCID: PMC7456874 DOI: 10.3389/fbioe.2020.00926] [Citation(s) in RCA: 43] [Impact Index Per Article: 8.6] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 06/14/2020] [Accepted: 07/20/2020] [Indexed: 11/13/2022] Open
Abstract
Rapid hemostasis and formation of stable blood clots are very important to prevent massive blood loss from the excessive bleeding for living body, but their own clotting process cannot be completed in time for effective hemostasis without the help of hemostatic materials. In general, traditionally suturing and stapling techniques for wound closure are prone to cause the additional damages to the tissues, activated inflammatory responses, short usage periods and inevitable second operations in clinical applications. Especially for the large wounds that require the urgent closure of fluids or gases, these conventional closure methods are far from enough. To address these problems, various tissue adhesives, sealants and hemostatic materials are placed great expectation. In this review, we focused on the development of two main categories of tissue adhesive materials: synthetic polymeric adhesives and naturally derived polysaccharide adhesives. Research of the high performance of hemostatic adhesives with strong adhesion, better biocompatibility, easy usability and cheap price is highly demanded for both scientists and clinicians, and this review is also intended to provide a comprehensive summarization and inspiration for pursuit of more advanced hemostatic adhesives for biological fields.
Collapse
Affiliation(s)
- Dawei Li
- Eighth Medical Center of the General Hospital of the Chinese People’s Liberation Army, Beijing, China
| | - Jing Chen
- Department of Orthopedics, Aerospace Center Hospital, Beijing, China
| | - Xing Wang
- Beijing National Laboratory for Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing, China
- University of Chinese Academy of Sciences, Beijing, China
| | - Mingming Zhang
- The People’s Liberation Army Strategic Support Force Characteristic Medical Center, Beijing, China
| | - Chunlin Li
- Eighth Medical Center of the General Hospital of the Chinese People’s Liberation Army, Beijing, China
| | - Jin Zhou
- Eighth Medical Center of the General Hospital of the Chinese People’s Liberation Army, Beijing, China
| |
Collapse
|
|
34
|
Martino C, Salzano F, Martino D, Ralli M, De Vincentiis M, Maranzano M, Greco A, Salzano G, Di Stadio A. A Prospective Randomized Trial of N-butyl-cyanoacrylate + Metacryloxysulfolane Adhesive versus Suture Alone for Grafting in Rhinoplasty: 9 year Follow-up. Ann Otol Rhinol Laryngol 2020; 130:483-489. [PMID: 32693615 DOI: 10.1177/0003489420943910] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/17/2022]
Abstract
OBJECTIVE Use of cyanoacrylate glue in facial plastic surgery is still controversial due to the absence of long-term follow up showing the results. Aim of our study is comparing the long-term outcomes of N-butyl-cyanoacrylate + Metacryloxysulfolane versus traditional sutures in rhinoplasty. METHODS Prospective comparative study. One hundred forty-two patients affected by ptotic nasal tip were included and randomized in two groups. In group A, the surgeon fixed the graft by using the glue and suture and in group B by using the traditional suture only. The following data were collected and compared by statistical analysis: nasolabial angle before and after surgery, dimensions of the graft, duration time (in minutes) for graft application during the surgery, number of sutures applied to fix the graft, presence of post-surgery negative outcomes. RESULTS All patients statistically improved their nasolabial angle after surgery (ANOVA: P < .0001) without statistically significant differences between the two groups both at short and long follow-up (χ:P = 1 and P = .9 respectively). A statistically significant difference in graft fixation time (P < .00001) and number of sutures (t: P < .00001) used was observed between the two groups. No statistically significant difference was observed in prevalence of infection after surgery. CONCLUSION N-butyl-cyanoacrylate + Metacryloxysulfolane could be a valid tool to reduce the necessary number of sutures and to reduce the time required for graft fixation graft fixation with consistent results in long-term follow-up.
Collapse
Affiliation(s)
- Carmine Martino
- University Hospital of Salerno, Italy.,The nose clinic, rhinosurgery unit c/o check-up day-surgery Salerno, Italy
| | | | - Diletta Martino
- The nose clinic, rhinosurgery unit c/o check-up day-surgery Salerno, Italy
| | - Massimo Ralli
- Organ of Sense Department, La Sapienza University of Rome, Italy
| | | | - Massimo Maranzano
- Department of Oral Maxillo-Facial and Facial Plastic Surgery, University of Manchester, Manchester, UK
| | - Antonio Greco
- Organ of Sense Department, La Sapienza University of Rome, Italy
| | - Giovanni Salzano
- Head and Neck Department, Neurosciences, Reproductive and Odontostomatological Science, University Federico II, Naples, Italy
| | | |
Collapse
|
|
35
|
Ptaszek LM, Portillo Lara R, Shirzaei Sani E, Xiao C, Roh J, Yu X, Ledesma PA, Hsiang Yu C, Annabi N, Ruskin JN. Gelatin Methacryloyl Bioadhesive Improves Survival and Reduces Scar Burden in a Mouse Model of Myocardial Infarction. J Am Heart Assoc 2020; 9:e014199. [PMID: 32458746 PMCID: PMC7428984 DOI: 10.1161/jaha.119.014199] [Citation(s) in RCA: 14] [Impact Index Per Article: 2.8] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Grants] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Indexed: 01/22/2023]
Abstract
Background Delivery of hydrogels to the heart is a promising strategy for mitigating the detrimental impact of myocardial infarction (MI). Challenges associated with the in vivo delivery of currently available hydrogels have limited clinical translation of this technology. Gelatin methacryloyl (GelMA) bioadhesive hydrogel could address many of the limitations of available hydrogels. The goal of this proof-of-concept study was to evaluate the cardioprotective potential of GelMA in a mouse model of MI. Methods and Results The physical properties of GelMA bioadhesive hydrogel were optimized in vitro. Impact of GelMA bioadhesive hydrogel on post-MI recovery was then assessed in vivo. In 20 mice, GelMA bioadhesive hydrogel was applied to the epicardial surface of the heart at the time of experimental MI. An additional 20 mice underwent MI but received no GelMA bioadhesive hydrogel. Survival rates were compared for GelMA-treated and untreated mice. Left ventricular function was assessed 3 weeks after experimental MI with transthoracic echocardiography. Left ventricular scar burden was measured with postmortem morphometric analysis. Survival rates at 3 weeks post-MI were 89% for GelMA-treated mice and 50% for untreated mice (P=0.011). Left ventricular contractile function was better in GelMA-treated than untreated mice (fractional shortening 37% versus 26%, P<0.001). Average scar burden in GelMA-treated mice was lower than in untreated mice (6% versus 22%, P=0.017). Conclusions Epicardial GelMA bioadhesive application at the time of experimental MI was performed safely and was associated with significantly improved post-MI survival compared with control animals. In addition, GelMA treatment was associated with significantly better preservation of left ventricular function and reduced scar burden.
Collapse
Affiliation(s)
- Leon M Ptaszek
- Cardiac Arrhythmia Service Massachusetts General Hospital Boston MA
| | - Roberto Portillo Lara
- Cardiac Arrhythmia Service Massachusetts General Hospital Boston MA
- Department of Chemical Engineering Northeastern University Boston MA
- Tecnologico de Monterrey Escuela de Ingeniera y Ciensias Zapopan Mexico
| | - Ehsan Shirzaei Sani
- Department of Chemical Engineering Northeastern University Boston MA
- Department of Chemical and Biomolecular Engineering University of California, Los Angeles CA
| | - Chunyang Xiao
- Cardiovascular Research Center Massachusetts General Hospital Boston MA
| | - Jason Roh
- Cardiovascular Research Center Massachusetts General Hospital Boston MA
| | - Xuejing Yu
- Cardiac Arrhythmia Service Massachusetts General Hospital Boston MA
| | - Pablo A Ledesma
- Cardiac Arrhythmia Service Massachusetts General Hospital Boston MA
| | - Chu Hsiang Yu
- Department of Chemical Engineering Northeastern University Boston MA
| | - Nasim Annabi
- Department of Chemical Engineering Northeastern University Boston MA
- Department of Chemical and Biomolecular Engineering University of California, Los Angeles CA
- Center for Minimally Invasive Therapeutics California NanoSystems Institute University of California, Los Angeles CA
| | - Jeremy N Ruskin
- Cardiac Arrhythmia Service Massachusetts General Hospital Boston MA
| |
Collapse
|
|
36
|
Yolgosteren A, Kumtepe G, Payaslioglu M, Ozakin C. In-vivo evaluation of the effect of cyanoacrylate on prosthetic vascular graft infection – does cyanoacrylate increase the severity of infection? VASA 2020; 49:281-284. [DOI: 10.1024/0301-1526/a000867] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.2] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/19/2022]
Abstract
Summary. Background: Prosthetic vascular graft infection (PVGI) is a complication with high mortality. Cyanoacrylate (CA) is an adhesive which has been used in a number of surgical procedures. In this in-vivo study, we aimed to evaluate the relationship between PVGI and CA. Materials and methods: Thirty-two rats were equally divided into four groups. Pouch was formed on back of rats until deep fascia. In group 1, vascular graft with polyethyleneterephthalate (PET) was placed into pouch. In group 2, MRSA strain with a density of 1 ml 0.5 MacFarland was injected into pouch. In group 3, 1 cm 2 vascular graft with PET piece was placed into pouch and MRSA strain with a density of 1 ml 0.5 MacFarland was injected. In group 4, 1 cm 2 vascular graft with PET piece impregnated with N-butyl cyanoacrylate-based adhesive was placed and MRSA strain with a density of 1 ml 0.5 MacFarland was injected. All rats were scarified in 96th hour, culture samples were taken where intervention was performed and were evaluated microbiologically. Bacteria reproducing in each group were numerically evaluated based on colony-forming unit (CFU/ml) and compared by taking their average. Results: MRSA reproduction of 0 CFU/ml in group 1, of 1410 CFU/ml in group 2, of 180 200 CFU/ml in group 3 and of 625 300 CFU/ml in group 4 was present. A statistically significant difference was present between group 1 and group 4 (p < 0.01), between group 2 and group 4 (p < 0.01), between group 3 and group 4 (p < 0.05). In terms of reproduction, no statistically significant difference was found in group 1, group 2, group 3 in themselves. Conclusions: We observed that the rate of infection increased in the cyanoacyrylate group where cyanoacrylate was used. We think that surgeon should be more careful in using CA in vascular surgery.
Collapse
Affiliation(s)
- Atıf Yolgosteren
- Cardiovascular Surgery, Gorukle Kampus/Uludag University, Bursa, Turkey
| | - Gencehan Kumtepe
- Department of Cardiovascular Surgery, Isparta City State Hospital, Isparta, Turkey
| | - Melda Payaslioglu
- Faculty of Medicine, Department of Microbiology, Uludag University, Bursa, Turkey
| | - Cuneyt Ozakin
- Faculty of Medicine, Department of Microbiology, Uludag University, Bursa, Turkey
| |
Collapse
|
|
37
|
Lee D, Hwang H, Kim JS, Park J, Youn D, Kim D, Hahn J, Seo M, Lee H. VATA: A Poly(vinyl alcohol)- and Tannic Acid-Based Nontoxic Underwater Adhesive. ACS APPLIED MATERIALS & INTERFACES 2020; 12:20933-20941. [PMID: 32233363 DOI: 10.1021/acsami.0c02037] [Citation(s) in RCA: 81] [Impact Index Per Article: 16.2] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 06/11/2023]
Abstract
Few studies aiming to develop a glue with an underwater reusable adhesive property have been reported because combining the two properties of reusable adhesion and underwater adhesion into a single glue formulation is a challenging issue. Herein, preparation of a simple mixture of poly(vinyl alcohol) (PVA) and a well-known phenolic compound, namely, tannic acid (TA), results in an underwater glue exhibiting reusable adhesion. We named the adhesive VATA (PVA + TA). Using VATA, two stainless steel objects (0.77 kg each) are able to be instantly attached. In addition to the high adhesive strength, surface-applied VATA in water retains its adhesive capability even after 24 h. In contrast, cyanoacrylate applied under the same water condition rapidly loses its adhesive power. Another advantage is that VATA's adhesion is reusable. Bonded objects can be forcibly detached, and then the detached ones can be reattached by the residual VATA. VATA maintains nearly 100% of its initial adhesive force, even after 10 repetitions of attach-detach cycles. VATA bonds various materials ranging from metals and polymers to ceramics. Particularly, we first attempt to test the toxicity of the underwater adhesives using an invertebrate nematode, Caenorhabditis elegans and gold fish (vertebrate) due to potential release to the environment.
Collapse
Affiliation(s)
- Daiheon Lee
- Department of Chemistry, Korea Advanced Institute of Science and Technology (KAIST), 291 University Road, Daejeon 34141, South Korea
| | - Honggu Hwang
- Department of Chemistry, Korea Advanced Institute of Science and Technology (KAIST), 291 University Road, Daejeon 34141, South Korea
- KOLON Advanced Research Cluster, KOLON One & Only Tower, 110, Magokdong-ro, Gangseo-gu, Seoul 07793, South Korea
| | - Jun-Sung Kim
- Department of Chemistry, Korea Advanced Institute of Science and Technology (KAIST), 291 University Road, Daejeon 34141, South Korea
| | - Jongmin Park
- Department of Chemistry, Korea Advanced Institute of Science and Technology (KAIST), 291 University Road, Daejeon 34141, South Korea
| | - Donghwan Youn
- Department of Chemistry, Korea Advanced Institute of Science and Technology (KAIST), 291 University Road, Daejeon 34141, South Korea
| | - Duhwan Kim
- KOLON Advanced Research Cluster, KOLON One & Only Tower, 110, Magokdong-ro, Gangseo-gu, Seoul 07793, South Korea
| | - Jungseok Hahn
- KOLON Advanced Research Cluster, KOLON One & Only Tower, 110, Magokdong-ro, Gangseo-gu, Seoul 07793, South Korea
| | - Myungeun Seo
- Department of Chemistry, Korea Advanced Institute of Science and Technology (KAIST), 291 University Road, Daejeon 34141, South Korea
| | - Haeshin Lee
- Department of Chemistry, Korea Advanced Institute of Science and Technology (KAIST), 291 University Road, Daejeon 34141, South Korea
| |
Collapse
|
|
38
|
Sun LC, Su Y, Ding XC, Xu DS, Li CM, Wang L, Li WL, Sun XD, Yu JM, Meng X. In vitro and in vivo evaluation of the safety and efficacy of a novel liquid fiducial marker for image-guided radiotherapy. Oncol Lett 2020; 20:569-580. [PMID: 32565982 PMCID: PMC7286123 DOI: 10.3892/ol.2020.11591] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.2] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 05/07/2019] [Accepted: 03/26/2020] [Indexed: 12/30/2022] Open
Abstract
The true extent of a tumor is difficult to visualize, during radiotherapy, using current modalities. In the present study, the safety and feasibility of a mixture of N-butyl cyanoacrylate and lipiodol (NBCA/Lip) was evaluated in order to investigate the optimal combination for application as a fiducial marker for radiotherapy. Four combinations of NBCA/Lip injection (1:1–0.1, 1:1–0.15, 1:3–0.1 and 1:3–0.15 ml) were injected into the subcutaneous tissue of BALB/c mice. The changes in gross histopathology, body weight, skin score, marker volume, neutrophil and macrophage counts were observed to analyze the effects of the different mixing ratios and injection volumes, in order to identify the best combination. Evaluation according to the International Organization for Standardization criteria was further conducted in order to test the biocompatibility of the mixture, including an acute systematic assay with mice, cytotoxicity with L929 fibroblasts and delayed-type hypersensitivity tests with guinea pigs and an intradermal test with rabbits. The results revealed that at the seventh week, 42 markers (42/48; 87.5%) were still visible using computed tomography (CT) imaging. No serious adverse effects were observed throughout the study period; however, the combination of 1:1–0.1 ml had the lowest body weight and worst skin score. A review of the histopathological reaction to NBCA/Lip revealed a combination of acute inflammation, chronic inflammation, granulation tissue, foreign-body reaction and fibrous capsule formation. The 1:1 NBCA combination ratio resulted in the most intense tissue repair reaction and a slower degradation rate of markers. In general, the combination of 1:3–0.15 ml had a better fusion with local tissue, maintained a stable imaging nodule on CT images for 7 weeks and the final biocompatibility test demonstrated its safety. Overall, the findings of the present study demonstrated NBCA/Lip as a safe and feasible fiducial marker, when using the 1:3–0.15 ml combination.
Collapse
Affiliation(s)
- Liang-Chao Sun
- Department of Radiation Oncology and Key Laboratory of Cancer Prevention and Therapy, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Tianjin's Clinical Research Center for Cancer, Tianjin, Shandong 300060, P.R. China.,Department of Radiation Oncology, Shandong Cancer Hospital and Institute, Shandong First Medical University and Shandong Academy of Medical Sciences, Jinan, Shandong 250117, P.R. China
| | - Yi Su
- Department of Radiotherapy, The Affiliated Yantai Yuhuangding Hospital of Qingdao University Institution, Yantai, Shandong 264001, P.R. China
| | - Xing-Chen Ding
- Department of Radiation Oncology, Shandong Cancer Hospital and Institute, Shandong First Medical University and Shandong Academy of Medical Sciences, Jinan, Shandong 250117, P.R. China
| | - Dong-Shui Xu
- Department of Radiation Oncology, Shandong Cancer Hospital and Institute, Shandong First Medical University and Shandong Academy of Medical Sciences, Jinan, Shandong 250117, P.R. China
| | - Cheng-Ming Li
- Department of Radiation Oncology, Shandong Cancer Hospital and Institute, Shandong First Medical University and Shandong Academy of Medical Sciences, Jinan, Shandong 250117, P.R. China
| | - Lu Wang
- Department of Radiation Oncology, Shandong Cancer Hospital and Institute, Shandong First Medical University and Shandong Academy of Medical Sciences, Jinan, Shandong 250117, P.R. China
| | - Wan-Long Li
- Department of Radiation Oncology, Shandong Cancer Hospital and Institute, Shandong First Medical University and Shandong Academy of Medical Sciences, Jinan, Shandong 250117, P.R. China
| | - Xin-Dong Sun
- Department of Radiation Oncology, Shandong Cancer Hospital and Institute, Shandong First Medical University and Shandong Academy of Medical Sciences, Jinan, Shandong 250117, P.R. China
| | - Jin-Ming Yu
- Department of Radiation Oncology, Shandong Cancer Hospital and Institute, Shandong First Medical University and Shandong Academy of Medical Sciences, Jinan, Shandong 250117, P.R. China
| | - Xue Meng
- Department of Radiation Oncology, Shandong Cancer Hospital and Institute, Shandong First Medical University and Shandong Academy of Medical Sciences, Jinan, Shandong 250117, P.R. China
| |
Collapse
|
|
39
|
The cytotoxic properties and apoptotic potential of N-butyl and 2-octyl cyanoacrylates used in surgical treatment of chronic venous insufficiency. TURK GOGUS KALP DAMAR CERRAHISI DERGISI-TURKISH JOURNAL OF THORACIC AND CARDIOVASCULAR SURGERY 2020; 27:185-191. [PMID: 32082851 DOI: 10.5606/tgkdc.dergisi.2019.17091] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.2] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Subscribe] [Scholar Register] [Received: 09/19/2018] [Accepted: 02/06/2019] [Indexed: 11/21/2022]
Abstract
Background This study aims to investigate the cytotoxic effects and apoptotic potential of N-butyl cyanoacrylate and 2-octyl cyanoacrylate used in surgical treatment of chronic venous insufficiency. Methods N-butyl cyanoacrylate and 2-octyl cyanoacrylate were cultured in cell-culture using human umbilical endothelial cell-line. Cytotoxicity and viability were assessed at 24 and 72 hours with lactate dehydrogenase and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assays, respectively. Apoptotic potential was documented at 24 and 72 hours with relative caspase-3 activity. Results The mean cytotoxicity at 24 and 72 hours were: N-butyl cyanoacrylate with an area of dot/line: 37.0±3.9%/29.3±2.7% and 46.4±1.6%/45.1±7.1%, 2-octyl cyanoacrylate with an area of dot/line: 39.0±7.0%/37.3±4.6% and 47.0±2.3%/40.7±7.5%. Cytotoxicity increased by time in each group (p<0.05). The mean viability at 24 and 72 hours were: N-butyl cyanoacrylate with an area of dot/line: 53.4±7.7%/72.0±5.7% and 35.7±1.9%/37.8±3.7%, 2-octyl cyanoacrylate with an area of dot/line: 54.3±4.4%/73.5±19.9% and 33.6±2.8%/30.7±4.5%. The mean viability decreased by time in each group (p<0.05). The mean relative caspase-3 activity at 24 and 72 hours were: control group: 0.084±0.006 and 0.065±0.002, N-butyl cyanoacrylate with an area of dot/line: 0.940±0.037/0.924±0.053 and 0.999±0.072/1.056±0.015, 2-octyl cyanoacrylate with an area of dot/line: 0.900±0.044/0.928±0.018 and 0.989±0.084/0.999±0.072. The mean relative caspase-3 activity was higher than control group in each group at each time interval (p<0.05) and activity increased by time in N-butyl cyanoacrylate line and in 2-octyl cyanoacrylate line groups (p<0.05). Conclusion Our findings indicate that N-butyl cyanoacrylate and 2-octyl cyanoacrylate cause cytotoxicity in cell-culture media. We may also postulate that they induce apoptosis in cell-culture media.
Collapse
|
|
40
|
Kapanya A, Somsunan R, Molloy R, Jiranusornkul S, Leewattanapasuk W, Jongpaiboonkit L, Kong Y. Synthesis of polymeric hydrogels incorporating chlorhexidine gluconate as antibacterial wound dressings. JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION 2020; 31:895-909. [DOI: 10.1080/09205063.2020.1725862] [Citation(s) in RCA: 4] [Impact Index Per Article: 0.8] [Reference Citation Analysis] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 10/25/2022]
Affiliation(s)
- Apichaya Kapanya
- Faculty of Science, Department of Chemistry, Chiang Mai University, Chiang Mai, Thailand
| | - Runglawan Somsunan
- Faculty of Science, Department of Chemistry, Chiang Mai University, Chiang Mai, Thailand
- Center of Excellence in Materials Science and Technology, Chiang Mai University, Chiang Mai, Thailand
| | - Robert Molloy
- Faculty of Science, Materials Science Research Center, Chiang Mai University, Chiang Mai, Thailand
| | - Supat Jiranusornkul
- Laboratory for Molecular Design and Simulation (LMDS), Department of Pharmaceutical Sciences, Faculty of Pharmacy, Chiang Mai University, Chiang Mai, Thailand
| | | | | | - Yen Kong
- AP Solutions LLC, Ypsilanti, MI, USA
| |
Collapse
|
|
41
|
Dompé M, Cedano-Serrano FJ, Vahdati M, Hourdet D, van der Gucht J, Kamperman M, Kodger TE. Hybrid Complex Coacervate. Polymers (Basel) 2020; 12:E320. [PMID: 32033133 PMCID: PMC7077495 DOI: 10.3390/polym12020320] [Citation(s) in RCA: 7] [Impact Index Per Article: 1.4] [Reference Citation Analysis] [Abstract] [Key Words] [Grants] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 12/17/2019] [Revised: 01/14/2020] [Accepted: 01/22/2020] [Indexed: 12/13/2022] Open
Abstract
Underwater adhesion represents a huge technological challenge as the presence of water compromises the performance of most commercially available adhesives. Inspired by natural organisms, we have designed an adhesive based on complex coacervation, a liquid-liquid phase separation phenomenon. A complex coacervate adhesive is formed by mixing oppositely charged polyelectrolytes bearing pendant thermoresponsive poly(N-isopropylacrylamide) (PNIPAM) chains. The material fully sets underwater due to a change in the environmental conditions, namely temperature and ionic strength. In this work, we incorporate silica nanoparticles forming a hybrid complex coacervate and investigate the resulting mechanical properties. An enhancement of the mechanical properties is observed below the PNIPAM lower critical solution temperature (LCST): this is due to the formation of PNIPAM-silica junctions, which, after setting, contribute to a moderate increase in the moduli and in the adhesive properties only when applying an ionic strength gradient. By contrast, when raising the temperature above the LCST, the mechanical properties are dominated by the association of PNIPAM chains and the nanofiller incorporation leads to an increased heterogeneity with the formation of fracture planes at the interface between areas of different concentrations of nanoparticles, promoting earlier failure of the network-an unexpected and noteworthy consequence of this hybrid system.
Collapse
Affiliation(s)
- Marco Dompé
- Laboratory of Physical Chemistry and Soft Matter, Wageningen University & Research, 6708 WE Wageningen, The Netherlands; (M.D.); (J.v.d.G.); (M.K.)
| | - Francisco Javier Cedano-Serrano
- Soft Matter Sciences and Engineering, ESPCI Paris, PSL University, Sorbonne University, CNRS, F-75005 Paris, France; (F.J.C.-S.); (M.V.); (D.H.)
| | - Mehdi Vahdati
- Soft Matter Sciences and Engineering, ESPCI Paris, PSL University, Sorbonne University, CNRS, F-75005 Paris, France; (F.J.C.-S.); (M.V.); (D.H.)
| | - Dominique Hourdet
- Soft Matter Sciences and Engineering, ESPCI Paris, PSL University, Sorbonne University, CNRS, F-75005 Paris, France; (F.J.C.-S.); (M.V.); (D.H.)
| | - Jasper van der Gucht
- Laboratory of Physical Chemistry and Soft Matter, Wageningen University & Research, 6708 WE Wageningen, The Netherlands; (M.D.); (J.v.d.G.); (M.K.)
| | - Marleen Kamperman
- Laboratory of Physical Chemistry and Soft Matter, Wageningen University & Research, 6708 WE Wageningen, The Netherlands; (M.D.); (J.v.d.G.); (M.K.)
- Laboratory of Polymer Science, Zernike Institute for Advanced Materials, University of Groningen, 9747 AG Groningen, The Netherlands
| | - Thomas E. Kodger
- Laboratory of Physical Chemistry and Soft Matter, Wageningen University & Research, 6708 WE Wageningen, The Netherlands; (M.D.); (J.v.d.G.); (M.K.)
| |
Collapse
|
|
42
|
Degen GD, Stow PR, Lewis RB, Andresen Eguiluz RC, Valois E, Kristiansen K, Butler A, Israelachvili JN. Impact of Molecular Architecture and Adsorption Density on Adhesion of Mussel-Inspired Surface Primers with Catechol-Cation Synergy. J Am Chem Soc 2019; 141:18673-18681. [DOI: 10.1021/jacs.9b04337] [Citation(s) in RCA: 30] [Impact Index Per Article: 5.0] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/28/2022]
|
|
43
|
Goldis A, Goldis R, Chirila TV. Biomaterials in Gastroenterology: A Critical Overview. ACTA ACUST UNITED AC 2019; 55:medicina55110734. [PMID: 31726779 PMCID: PMC6915447 DOI: 10.3390/medicina55110734] [Citation(s) in RCA: 6] [Impact Index Per Article: 1.0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Download PDF] [Journal Information] [Subscribe] [Scholar Register] [Received: 09/26/2019] [Revised: 10/30/2019] [Accepted: 11/08/2019] [Indexed: 02/07/2023]
Abstract
In spite of the large diversity of diagnostic and interventional devices associated with gastrointestinal endoscopic procedures, there is little information on the impact of the biomaterials (metals, polymers) contained in these devices upon body tissues and, indirectly, upon the treatment outcomes. Other biomaterials for gastroenterology, such as adhesives and certain hemostatic agents, have been investigated to a greater extent, but the information is fragmentary. Much of this situation is due to the paucity of details disclosed by the manufacturers of the devices. Moreover, for most of the applications in the gastrointestinal (GI) tract, there are no studies available on the biocompatibility of the device materials when in intimate contact with mucosae and other components of the GI tract. We have summarized the current situation with a focus on aspects of biomaterials and biocompatibility related to the device materials and other agents, with an emphasis on the GI endoscopic procedures. Procedures and devices used for the control of bleeding, for polypectomy, in bariatrics, and for stenting are discussed, particularly dwelling upon the biomaterial-related features of each application. There are indications that research is progressing steadily in this field, and the establishment of the subdiscipline of "gastroenterologic biomaterials" is not merely a remote projection. Upon the completion of this article, the gastroenterologist should be able to understand the nature of biomaterials and to achieve a suitable and beneficial perception of their significance in gastroenterology. Likewise, the biomaterialist should become aware of the specific tasks that the biomaterials must fulfil when placed within the GI tract, and regard such applications as both a challenge and an incentive for progressing the research in this field.
Collapse
Affiliation(s)
- Adrian Goldis
- Faculty of Medicine, Victor Babes University of Medicine and Pharmacy, 300041 Timisoara, Romania
- Correspondence:
| | | | - Traian V. Chirila
- Queensland Eye Institute, South Brisbane, QL 4101, Australia;
- Science & Engineering Faculty, Queensland University of Technology, Brisbane, QL 4000, Australia
- Faculty of Medicine, University of Queensland, Herston, QL 4029, Australia
- Australian Institute for Bioengineering and Nanotechnology, University of Queensland, St Lucia, 4072 QL, Australia
- Faculty of Science, University of Western Australia, Crawley, WA 6009, Australia
- University of Medicine, Pharmacy, Sciences and Technology, 540139 Targu Mures, Romania
| |
Collapse
|
|
44
|
Krishnadoss V, Melillo A, Kanjilal B, Hannah T, Ellis E, Kapetanakis A, Hazelton J, San Roman J, Masoumi A, Leijten J, Noshadi I. Bioionic Liquid Conjugation as Universal Approach To Engineer Hemostatic Bioadhesives. ACS APPLIED MATERIALS & INTERFACES 2019; 11:38373-38384. [PMID: 31523968 DOI: 10.1021/acsami.9b08757] [Citation(s) in RCA: 35] [Impact Index Per Article: 5.8] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 06/10/2023]
Abstract
Adhesion to wet and dynamic surfaces is vital for many biomedical applications. However, the development of effective tissue adhesives has been challenged by the required combination of properties, which includes mechanical similarity to the native tissue, high adhesion to wet surfaces, hemostatic properties, biodegradability, high biocompatibility, and ease of use. In this study, we report a novel bioinspired design with bioionic liquid (BIL) conjugated polymers to engineer multifunctional highly sticky, biodegradable, biocompatible, and hemostatic adhesives. Choline-based BIL is a structural precursor of the phospholipid bilayer in the cell membrane. We show that the conjugation of choline molecules to naturally derived polymers (i.e., gelatin) and synthetic polymers (i.e., polyethylene glycol) significantly increases their adhesive strength and hemostatic properties. Synthetic or natural polymers and BILs were mixed at room temperature and cross-linked via visible light photopolymerization to make hydrogels with tunable mechanical, physical, adhesive, and hemostatic properties. The hydrogel adhesive exhibits a close to 50% decrease in the total blood volume loss in tail cut and liver laceration rat animal models compared to the control. This technology platform for adhesives is expected to have further reaching application vistas from tissue repair to wound dressings and the attachment of flexible electronics.
Collapse
Affiliation(s)
| | - Atlee Melillo
- Cooper Medical School of Rowan University , Camden , New Jersey 08103-1211 , United States
| | | | | | | | | | - Joshua Hazelton
- Cooper Medical School of Rowan University , Camden , New Jersey 08103-1211 , United States
| | - Janika San Roman
- Cooper Medical School of Rowan University , Camden , New Jersey 08103-1211 , United States
| | | | - Jeroen Leijten
- Developmental BioEngineering (DBE) , The University of Twente , 7522 NB Enschede , Netherlands
| | | |
Collapse
|
|
45
|
Cyanacrylate Glue Caused Extrinsic Compression of an Infrapopliteal Vein Graft. Ann Vasc Surg 2019; 63:460.e5-460.e8. [PMID: 31629841 DOI: 10.1016/j.avsg.2019.09.020] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 07/22/2019] [Revised: 08/30/2019] [Accepted: 09/01/2019] [Indexed: 11/21/2022]
Abstract
Several surgical sealant devices are commercially available after their rigorous clinical testing with no apparent complications reported so far in the current literature. Cyanoacrylate glue can be used to stabilize the anastomoses and permit a better tensile strength in cardiovascular surgery. We first report the case of a 71-year-old male patient presenting with symptoms of progressive limitation of walking distance, 13 months after a successful femoroinfrapopliteal bypass surgery, because of a calcified tissue extrinsically stenosizing the first segment of the previous bypass graft, caused by the use of cyanoacrylate glue.
Collapse
|
|
46
|
Chen B, Yang J, Bai R, Suo Z. Molecular Staples for Tough and Stretchable Adhesion in Integrated Soft Materials. Adv Healthc Mater 2019; 8:e1900810. [PMID: 31368256 DOI: 10.1002/adhm.201900810] [Citation(s) in RCA: 9] [Impact Index Per Article: 1.5] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 06/22/2019] [Revised: 07/16/2019] [Indexed: 01/26/2023]
Abstract
The integration of soft materials-biological tissues, gels, and elastomers-is a rapidly developing technology of this time. Whereas hard materials are adhered using adhesives of hard polymers since antiquity, these hard polymers are generally unsuited to adhere soft materials, because hard polymers constrain the deformation of soft materials. This paper describes a design principle to use hard polymers to adhere soft materials, such that adhesion remains tough after the adhered soft materials are subject to many cycles of large stretches in the plane of their interface. The two soft materials have stretchable polymer networks, but need not have functional groups for adhesion. The two soft materials are adhered by forming, in situ at their interface, islands of a hard polymer. The adhesion is tough if the islands themselves are strong, and the polymers of the islands are in topological entanglement with the polymer networks of the soft materials. The adhesion is stretchable if the islands are smaller than the flaw sensitivity length. Several methods of forming the hard polymer islands are demonstrated, and the mechanics and chemistry of adhesion are studied. The design principle will enable many hard polymers to form tough and stretchable adhesion between soft materials.
Collapse
Affiliation(s)
- Baohong Chen
- John A. Paulson School of Engineering and Applied SciencesKavli Institute for Bionano Science and TechnologyHarvard University Cambridge MA 02138 USA
| | - Jiawei Yang
- John A. Paulson School of Engineering and Applied SciencesKavli Institute for Bionano Science and TechnologyHarvard University Cambridge MA 02138 USA
| | - Ruobing Bai
- John A. Paulson School of Engineering and Applied SciencesKavli Institute for Bionano Science and TechnologyHarvard University Cambridge MA 02138 USA
| | - Zhigang Suo
- John A. Paulson School of Engineering and Applied SciencesKavli Institute for Bionano Science and TechnologyHarvard University Cambridge MA 02138 USA
| |
Collapse
|
|
47
|
Chen H, Shao S, Yu Y, Huang Y, Zhu X, Zhang S, Fan J, Yin GY, Chi B, Wan M, Mao C. A dual-responsive biosensor for blood lead detection. Anal Chim Acta 2019; 1093:131-141. [PMID: 31735206 DOI: 10.1016/j.aca.2019.09.062] [Citation(s) in RCA: 19] [Impact Index Per Article: 3.2] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 08/22/2019] [Accepted: 09/23/2019] [Indexed: 12/17/2022]
Abstract
Simple and accurate detection of trace heavy metals in blood is very important. A novel dual-responsive electrochemical/fluorescent biosensor based on magnetic hyperbranched polyamide with heparin modification (MHPAM-H) for blood lead detection has been successfully developed. Upon conjugated with blood lead ions, dual-biosensor could not only display electrochemical signal but also fluorescence signal owing to the enriched amino groups, cavity structure, and good fluorescence properties of HPAM. Blood biocompatibility, construction of the dual-responsive biosensor, electrochemical/fluorescent detection of lead ions in water phase and blood condition, selectivity and stability of the dual-responsive biosensor were investigated in detail. The proposed dual-responsive biosensor displays good linear relationship (1.5 pM- 4.8 × 103 pM for electrochemical detection and 0.5 pM-4.8 × 103 pM for fluorescent detection) with low detection limit (4.4 pM for electrochemical detection and 1.0 pM for fluorescent detection) for blood lead, providing potential application for blood lead detection in the future.
Collapse
Affiliation(s)
- Huan Chen
- National and Local Joint Engineering Research Center of Biomedical Functional Materials, School of Chemistry and Materials Science, Nanjing Normal University, Nanjing, 210023, China
| | - Shuibin Shao
- National and Local Joint Engineering Research Center of Biomedical Functional Materials, School of Chemistry and Materials Science, Nanjing Normal University, Nanjing, 210023, China
| | - Yueqi Yu
- National and Local Joint Engineering Research Center of Biomedical Functional Materials, School of Chemistry and Materials Science, Nanjing Normal University, Nanjing, 210023, China
| | - Yangyang Huang
- National and Local Joint Engineering Research Center of Biomedical Functional Materials, School of Chemistry and Materials Science, Nanjing Normal University, Nanjing, 210023, China
| | - Xiaotan Zhu
- National and Local Joint Engineering Research Center of Biomedical Functional Materials, School of Chemistry and Materials Science, Nanjing Normal University, Nanjing, 210023, China
| | - Shiyan Zhang
- National and Local Joint Engineering Research Center of Biomedical Functional Materials, School of Chemistry and Materials Science, Nanjing Normal University, Nanjing, 210023, China
| | - Jin Fan
- Department of Orthopaedics, The First Affiliated Hospital of Nanjing Medical University, Nanjing, 211166, China
| | - Guo Yong Yin
- Department of Orthopaedics, The First Affiliated Hospital of Nanjing Medical University, Nanjing, 211166, China
| | - Bo Chi
- State Key Laboratory of Materials-Oriented Chemical Engineering, College of Food Science and Light Industry, Nanjing Tech University, Nanjing, 211816, China
| | - Mimi Wan
- National and Local Joint Engineering Research Center of Biomedical Functional Materials, School of Chemistry and Materials Science, Nanjing Normal University, Nanjing, 210023, China.
| | - Chun Mao
- National and Local Joint Engineering Research Center of Biomedical Functional Materials, School of Chemistry and Materials Science, Nanjing Normal University, Nanjing, 210023, China.
| |
Collapse
|
|
48
|
Andreotti Damante C, Cardoso MV, Hage Karam PSB, Haiter AC, Sant'ana ACP, Greghi SLA, Zangrando MSR, De Rezende MLR, Oliveira RC. Evaluation of Regular Market Ethyl Cyanoacrylate Cytotoxicity for Human Gingival Fibroblasts and Osteoblasts. Surg Infect (Larchmt) 2019; 21:29-34. [PMID: 31397637 DOI: 10.1089/sur.2019.141] [Citation(s) in RCA: 3] [Impact Index Per Article: 0.5] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 12/26/2022] Open
Abstract
Background: The aim of this study was to evaluate the cytotoxicity of cyanoacrylate adhesives in an indirect contact assay in human gingival fibroblast (FGH) and oral osteoblasts (GO) lineages. Methods: Cover glasses were glued with adhesives following the ISO 10993-2012 protocol. The groups were: C (control with cells and regular Dulbecco Modified Eagle Medium; LC (liquid ethyl-cyanoacrylate); GC (ethyl-cyanoacrylate gel); EGC (easy gel [ethyl-cyanoacrylate]); and D (Dermabond [octyl-cyanoacrylate]). Each cell linage was plated in the sixth passage using 104 cells. Cell viability was measured by the MTT test at 24, 48, 72, and 96 hours. Data were analyzed by two-way analysis of variance complemented by the Tukey test, with p < 0.05 being significant. Results: Dermabond stimulated osteoblast viability at 72 h (p < 0.05). All other groups were similar to the control cells (p > 0.05). For the fibroblasts, there was no difference in the groups, including the control except that EGC was cytotoxic for these cells (p < 0.05). Conclusions: Ethyl-cyanoacrylate gel and liquid forms available on the general chemical market were not cytotoxic for oral osteoblasts and fibroblasts in most cases. However, the easy gel form was cytotoxic for fibroblasts.
Collapse
Affiliation(s)
- Carla Andreotti Damante
- Department of Periodontology, Bauru School of Dentistry, University of São Paulo, Bauru, Brazil
| | - Matheus Völz Cardoso
- Department of Periodontology, Bauru School of Dentistry, University of São Paulo, Bauru, Brazil
| | | | - Ana Carolina Haiter
- Department of Periodontology, Bauru School of Dentistry, University of São Paulo, Bauru, Brazil
| | | | | | | | | | | |
Collapse
|
|
49
|
Lacerda-Santos R, Roberto BMS, de Siqueira Nunes B, Carvalho FG, Dos Santos A, Dantas AFM. Histological analysis of biocompatibility of different surgical adhesives in subcutaneous tissue. Microsc Res Tech 2019; 82:1184-1190. [PMID: 30924586 DOI: 10.1002/jemt.23267] [Citation(s) in RCA: 6] [Impact Index Per Article: 1.0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 10/01/2018] [Revised: 01/07/2019] [Accepted: 02/27/2019] [Indexed: 11/09/2022]
Abstract
The focus of this study was to test the hypothesis that there would be no difference between the biocompatibility of cyanoacrylate-based adhesives in rat subcutaneous tissues. In total, 60 male Wistar rats were used, and divided into four groups (n = 15): Group C (control, PVA-polyvinyl alcohol sponge), Group NO (N-butyl-2-octylcyanoacrylate), Group NH (n-hexyl-cyanoacrylate), and Group EC (Ethyl-cyanoacrylate). The animals were sacrificed after time intervals of 7, 15, and 30 days and tissues were analyzed under optical microscope as regards the events of inflammatory infiltrate, edema, necrosis, granulation tissue, giant cells, young fibroblasts, and collagen formation. The results were statistically analyzed by the Kruskal-Wallis and Dunn tests (p < .05). Significant inflammatory infiltrate was shown for all the adhesives in the time intervals of 7 (p = .004) and 15 days (p = .003). In the time interval of 30 days, moderate inflammatory infiltrate was observed in Groups NH and EC, with significant difference from Control (p = .001). The quantity of collagen fibers in all the experimental groups showed significant difference compared with Control in the time intervals of 7 (p = .002) and 15 days (p = .001), at 30 days only Group EC showed a smaller quantity of collagen fibers in comparison with Control (p = .001). The hypothesis was rejected. The adhesive N-butyl-2-octylcyanoacrylate had less influence on the inflammatory intensity of multinucleated giant cells. Ethyl-cyanoacrylate demonstrated the lowest level of biocompatibility among the adhesives, but its use in clinical practice may be promising for coaptation of smaller edges of superficial tissue. Surgical adhesives were shown to be feasible for clinical use in substitution of conventional suturing. Ethyl-cyanoacrylate should be used with caution due to its greater influence on tissues.
Collapse
Affiliation(s)
- Rogério Lacerda-Santos
- Department of Orthodontics and Pediatric Dentistry, Dental School, Federal University of de Fora, Governador Valadares, Minas Gerais, Brazil
| | - Berthiene M S Roberto
- Department of Orthodontics and Pediatric Dentistry, Dental School, Federal University of Campina Grande, Patos, Paraíba, Brazil
| | - Bruna de Siqueira Nunes
- Department of Orthodontics and Pediatric Dentistry, Dental School, Federal University of Campina Grande, Patos, Paraíba, Brazil
| | - Fabiola G Carvalho
- Department of Orthodontics and Pediatric Dentistry, Dental School, Federal University of de Fora, Governador Valadares, Minas Gerais, Brazil
| | | | - Antônio F M Dantas
- Department of Pathology, School of Veterinary Medicine, Federal University of Campina Grande, Patos, Paraíba, Brazil
| |
Collapse
|
|
50
|
Chen LX, Coulombe M, Barthelat F, Rammal A, Mongeau L, Kost K. Investigation of surgical adhesives for vocal fold wound closure. Laryngoscope 2019; 129:2139-2146. [PMID: 30883775 DOI: 10.1002/lary.27769] [Citation(s) in RCA: 4] [Impact Index Per Article: 0.7] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 05/14/2018] [Revised: 11/19/2018] [Accepted: 11/26/2018] [Indexed: 01/11/2023]
Abstract
OBJECTIVES Surgical adhesives are increasingly used for vocal fold microsurgery to assist wound closure and reduce the risks of scar formation. Currently used vocal fold adhesives such as fibrin glue, however, have thus far not been found to promote wound closure or reduce scarring. The objectives of this study were to investigate the mechanical strength and the cytotoxicity of three commercially available adhesives (Glubran 2, GEM, Viareggio, Italy; BioGlue, CryoLife, Kennesaw, GA; and Tisseel, Baxter Healthcare, Deerfield, IL) for vocal fold wound closure. METHODS Shear and tension tests were performed on 150 porcine larynges. The cytotoxicity of the adhesives to immortalized human vocal fold fibroblasts was investigated using neutral red uptake assays. RESULTS The average shear adhesive strength for Tisseel, BioGlue, and Glubran 2 was 13.86 ± 5.03 kilopascal (kPa), 40.92 ± 17.94 kPa, and 68.79 ± 13.29 kPa, respectively. The tensile adhesive strength for Tisseel, BioGlue, and Glubran 2 was 10.70 ± 6.42 kPa, 34.27 ± 12.59 kPa, and 46.67 ± 12.13 kPa, respectively. The vocal fold cell viabilities in extracts of Tisseel, BioGlue, and Glubran 2 were 99.27%, 43.05%, and 1.79%, respectively. CONCLUSION There was a clear tradeoff between adhesive strength and toxicity. The maximum failure strength in shear or tension of the three surgical adhesives ranked from strongest to the weakest was: 1) Glubran 2, 2) BioGlue, and 3) Tisseel. Tisseel was found to be the least toxic of the three adhesives, whereas Glubran 2 was the most toxic. LEVEL OF EVIDENCE NA Laryngoscope, 129:2139-2146, 2019.
Collapse
Affiliation(s)
- Lei Xi Chen
- Department of Mechanical Engineering, McGill University, Montreal, Quebec, Canada
| | - Maxence Coulombe
- Department of Mechanical Engineering, McGill University, Montreal, Quebec, Canada
| | - Francois Barthelat
- Department of Mechanical Engineering, McGill University, Montreal, Quebec, Canada
| | - Almoaidbellah Rammal
- Department of Otolaryngology-Head and Neck Surgery, McGill University, Montreal, Quebec, Canada
| | - Luc Mongeau
- Department of Mechanical Engineering, McGill University, Montreal, Quebec, Canada
| | - Karen Kost
- Department of Otolaryngology-Head and Neck Surgery, McGill University, Montreal, Quebec, Canada
| |
Collapse
|