Based on the crucial role of boron in the metabolism of algae and bacteria, this study aimed to investigate the effects of boron on microalgae-bacterial granular sludge (MBGS) system. The addition of boron stimulated the secretion of autoinducer-2 to promote bacterial quorum sensing, which increased the extracellular polymeric substances production by more than 12.5 %. Meanwhile, boron significantly impacted the indole-3-acetic acid levels in microalgae to improve photosynthetic efficiency and increased the abundance of target algae (Chlorophyta and Cyanobacteria) by more than 0.23 times and 0.88 times, respectively. However, the influence of boron was not concentration-dependent, with the optimal concentration as 80 µM. Both boron deficiency and excess can heighten oxidative stress and affect the stability of the MBGS system. This study highlights the essentiality of boron in simultaneously influencing signal communication of algae and bacteria to strengthen synergy, and provides a theoretical basis for strengthening the symbiotic relationship within MBGS.

The substantial rise in global energy demand, propelled by industrial expansion, population growth, and transportation needs, poses a formidable challenge. The concurrent urbanization places pressure on the disposal of solid municipal solid waste and the management of plastic waste. Addressing the global waste crisis requires innovative and sustainable garbage disposal solutions with an environmentally friendly approach. This review tackles the challenges of worldwide waste management, focusing on renewable and sustainable fuels and waste recycling through the exploration of co-pyrolysis as an innovative method. It explores the characteristics and environmental impact of municipal solid waste (MSW) and plastic waste (PW), delving into pyrolysis fundamentals, processes, and challenges. The primary emphasis is on co-pyrolysis, elucidating its integration of municipal and plastic waste, synergistic effects, and advantages. The manuscript thoroughly analyzes reaction kinetics, thermodynamics, and the feasibility of co-pyrolysis for energy recovery. It also delves into the synthesis of renewable fuels and valuable chemical intermediates, considering optimization of product distribution. Environmental and economic sustainability aspects, including impact assessment, greenhouse gas emissions, life cycle analysis, and cost analysis of co-pyrolysis processes, are comprehensively investigated. The review underscores the economic benefits of renewable fuel and chemical materials synthesis. The conclusion addresses challenges, proposes future directions, outlines limitations, technical challenges, environmental considerations, and recommends further exploration and integration with other waste management techniques. The manuscript emphasizes the ongoing importance of research in this critical field, aiming to contribute to the development of effective solutions for the escalating global waste management crisis.

Hydrothermal pretreatment is useful for microfibrillated cellulose (MFC) preparation due to its safety, but the remaining hemicellulose might affect MFC properties. This study aimed to investigate the effect of centrifugation time on hemicellulose removal and the physicochemical properties of MFC obtained after hydrothermal pretreatment and micro-fibrillation. In this study, centrifugation was applied to the MFC suspension at varying duration times. Composition analysis and Fourier transform infrared spectra indicated that fractionated MFC has no hemicellulose content after 10, 20, and 30 min centrifugation. It also showed an approximately 5 times higher than 0.5 % g/g of initial solid concentration, indicated by a lower gel concentration point, than unfractionated MFC. Scanning electron microscope images of the fractionated MFC for 30 min (MFC2C) presented thin, long cellulose fibrils of 517 nm in average diameter and 635-10,000 nm in length that induced a slower sedimentation rate. MFC2C dispersion was also improved by autoclave sterilization by regulating cellulose structure, rheology, and crystallinity. As a result, MFC dispersibility can be enhanced by removing hemicellulose through simple centrifugation.

l-Histidine plays significant roles in the food and pharmaceutical industries, and its demand has been steadily increasing recently. As demand for l-histidine continues, the development of eco-friendly processes is required. To pursue this goal, D-galactose, a primary component of red algae, was employed as a carbon source for synthesizing l-histidine. To harness this marine biomass, κ-carrageenan was preferentially hydrolyzed to obtain D-galactose using κ-carrageenase (CgkA) and iduronate-2-sulfatase (IdsA3). Subsequently, l-histidine production was enhanced by modifying precursor pathways in Corynebacterium glutamicum. The resulting strain, TDPH6 exhibited a remarkable 2.15-fold increase in l-histidine production compared to TDP. Furthermore, a galactose utilization system was introduced and named TDPH6G2. During fermentation, this strain efficiently consumed 100 % of the D-galactose and synthesized 0.395 g/L of l-histidine. In conclusion, this study presents a sustainable approach to L-histidine synthesis by introducing a galactose utilization system into C. glutamicum.

Marine red algal biomass is a promising feedstock for sustainable production of value-added chemicals. However, the major constituents of red algal biomass, such as agar and carrageenan, are not easily assimilated by most industrial metabolic chassis developed to date. Synthetic biology offers a solution by utilizing nonmodel organisms as metabolic chassis for consolidated biological processes. In this study, the marine heterotrophic bacterium Pseudoalteromonas atlantica T6c was harnessed as a metabolic chassis to produce value-added chemicals from the affordable red algal galactans or agaropectin, a byproduct of industrial agarose production. To construct a heterologous gene expression device in P. atlantica T6c, promoters related to agar metabolism were screened from the differentially expressed genes using RNA-Seq analysis. The expression device was built and tested with selected promoters fused to a reporter gene and tuned by incorporation of a cognate repressor predicted from the agar-specific polysaccharide utilization locus. The feasibility of the marine bacterial metabolic chassis was examined by introducing the biosynthetic gene clusters of β-carotene and violacein. Our results demonstrate that the metabolic chassis platform enables direct conversion of low-cost red algal galactans or industrial waste agaropectin into valuable bioactive pigments without any pretreatment of biomass. The developed marine bacterial chassis could potentially be used in a biorefinery framework to produce value-added chemicals from marine algal galactans.

Marine herbivorous fish that feed primarily on macroalgae, such as those from the genus Kyphosus, are essential for maintaining coral health and abundance on tropical reefs. Here, deep metagenomic sequencing and assembly of gut compartment-specific samples from three sympatric, macroalgivorous Hawaiian kyphosid species have been used to connect host gut microbial taxa with predicted protein functional capacities likely to contribute to efficient macroalgal digestion. Bacterial community compositions, algal dietary sources, and predicted enzyme functionalities were analyzed in parallel for 16 metagenomes spanning the mid- and hindgut digestive regions of wild-caught fishes. Gene colocalization patterns of expanded carbohydrate (CAZy) and sulfatase (SulfAtlas) digestive enzyme families on assembled contigs were used to identify likely polysaccharide utilization locus associations and to visualize potential cooperative networks of extracellularly exported proteins targeting complex sulfated polysaccharides. These insights into the gut microbiota of herbivorous marine fish and their functional capabilities improve our understanding of the enzymes and microorganisms involved in digesting complex macroalgal sulfated polysaccharides. IMPORTANCE This work connects specific uncultured bacterial taxa with distinct polysaccharide digestion capabilities lacking in their marine vertebrate hosts, providing fresh insights into poorly understood processes for deconstructing complex sulfated polysaccharides and potential evolutionary mechanisms for microbial acquisition of expanded macroalgal utilization gene functions. Several thousand new marine-specific candidate enzyme sequences for polysaccharide utilization have been identified. These data provide foundational resources for future investigations into suppression of coral reef macroalgal overgrowth, fish host physiology, the use of macroalgal feedstocks in terrestrial and aquaculture animal feeds, and the bioconversion of macroalgae biomass into value-added commercial fuel and chemical products.

With the determination of the Leloir pathway in a solventogenic wild-type strain WK through the transcriptional analysis, two pivotal genes (galK and galT) were systematically co-expressed to demonstrate a significantly enhanced galactose utilization for butanol production with the elimination of carbon catabolite repression (CCR). The gene-modified strain WK-Gal-4 could effectively co-utilize galactose and glucose by directly using an ultrasonication-assisted butyric acid-pretreated Gelidium amansii hydrolysate (BAU) as the substrate, exhibiting the optimal sugar consumption and butanol production from BAU of 20.31 g/L and 7.8 g/L with an increment by 62.35 % and 61.49 % over that by strain WK, respectively. This work for the first time develops a feasible approach to utilizing red algal biomass for butanol fermentation through exploring the metabolic regulation of carbohydrate catabolism, also offering a novel route to develop the future biorefinery using the cost-effective and sustainable marine feedstocks.

Microalgae are a promising source of raw material (i.e., proteins, carbohydrates, lipids, pigments, and micronutrients) for various value-added products and act as a carbon sink for atmospheric CO₂. The rigidity of the microalgal cell wall makes it difficult to extract different cellular components for its applications, including biofuel production, food and feed supplements, and pharmaceuticals. To improve the recovery of products from microalgae, pretreatment strategies such as biological, physical, chemical, and combined methods have been explored to improve whole-cell disruption and product recovery efficiency. However, the diversity and uniqueness of the microalgal cell wall make the pretreatment process more species-specific and limit its large-scale application. Therefore, advancing the currently available technologies is required from an economic, technological, and environmental perspective. Thus, this paper provides a state-of-art review of the current trends, challenges, and prospects of sustainable microalgal pretreatment technologies from a microalgae-based biorefinery concept.

Biofuels from microalgal biomass is among some of the promising sustainable energy technologies that can significantly replace the dependence on fossil fuels worldwide due to potentiality to lower CO₂ emissions. Nevertheless, the extraction of biomolecules for biofuel generation is inhibited by the rigidity of the cellular structure of microalgal biomass. Various pretreatment strategies have been evaluated for their efficacy in microalgal cell wall disruption to enhance microalgal bioenergy production. However, the efficiency of the pretreatment methods depend on the particular species being treated due to the inherent variability of the composition of the cell wall. This paper reviews pretreatment strategies (mainly novel physical, chemical and physicochemical) employed in bioenergy generation from microalgal biomass, address existing constraints and provides prospects for economic and industrial-scale production. The authors have also discussed the different pretreatment methods used for biodiesel, bioethanol, and biohydrogen production.

The production of chemicals and energy from sustainable biomass with an important objective decreasing carbon impressions has recently become one of the key areas of attention. Algae biomass have been recognized and researched as a potential renewable biomass of biohydrogen production attributed to their limited multiplying time, fast growing qualities and ability of lipid accumulation. This review additionally envelops various key perspectives such as composition and properties of algae biomass and pretreatment strategies such as physical, chemical and biological methods adopted for the algae biomass. This review is mainly focused on pretreatment strategies which have been developed to enhance biohydrogen production. The present review deals with methods and mechanism, enzymes involved and factors influencing on biohydrogen production which help to grasp various bottlenecks, challenges and constraints. Finally, the significant progressions and economical perspective on improving biohydrogen yield because of the expansion of co-substrates and the current trends are examined.

Algal biorefinery is rising as a prominent solution to economically fulfill the escalating global requirement for nutrition, feed, fuel, and medicines. In recent years, scientific productiveness associated with microalgae-based studies has elaborated in multiplied aspects, while translation to the commercial level continues to be missing. The present microalgal biorefinery has a challenge in long-term viability due to escalated market price of algal-mediated biofuels and bioproducts. Advancements are required in a few aspects like improvement in algae processing, energy investment, and cost analysis of microalgae biorefinery. Therefore, it is essential to recognize the modern work by understanding the knowledge gaps and hotspots driving business scale up. The microalgae biorefinery integrated with energy-based products, bioactive and green compounds, focusing on a circular bioeconomy, is urgently needed. A detailed investigation of techno-economic analysis (TEA) and life cycle assessment (LCA) is important to increase the market value of algal products. This review discusses the valorization of algal biomass for the value-added application that holds a sustainable approach and cost-competitive algal biorefinery. The current industries, policies, technology transfer trends, challenges, and future economic outlook are discussed. This study is an overview through scientometric investigation attempt to describe the research development contributing to this rising field.

Urbanization and pollution are the major issues of the current time own to the exhaustive consumption of fossil fuels which have a detrimental effect on the nation's economies and air quality due to greenhouse gas (GHG) emissions and shortage of energy reserves. Algae, an autotrophic organism provides a green substitute for energy as well as commercial products. Algal extracts become an efficient source for bioactive compounds having anti-microbial, anti-oxidative, anti-inflammatory, and anti-cancerous potential. Besides the conventional approach, residual biomass from any algal-based process might act as a renewable substrate for fermentation. Likewise, lignocellulosic biomass, algal biomass can also be processed for sugar recovery by different pre-treatment strategies like acid and alkali hydrolysis, microwave, ionic liquid, and ammonia fiber explosion, etc. Residual algal biomass hydrolysate can be used as a feedstock to produce bioenergy (biohydrogen, biogas, methane) and biochemicals (organic acids, polyhydroxyalkanoates) via microbial fermentation.

A series of Brønsted acidic ionic liquids (BAILs) functionalized hollow organosilica nanospheres ([C_3/4Im][OTs/OTf]-Si(Et)Si, C_3/4 = Pr/BuSO₃H) were synthesized by two steps. The process involved the preparation of hollow nanosphere supports via a toluene-swollen sol-gel co-condensation of 1,2-bis(trimethoxysilyl)ethane and 3-chloropropyltriethoxysilane in the presence of F127, and followed by a successive quaternary ammonization and protonation with imidazole, 1,3-propane/1,4-butane sultone and trifluoromethane sulfonic acid/p-toluenesulfonic acid. The adjustable acid property, hollow inner diameter (5-15 nm) and shell thickness (5-9 nm) of [C_3/4Im][OTs/OTf]-Si(Et)Si are achieved by introducing different organic acids and controlling toluene concentration, respectively. The [C_3/4Im][OTs/OTf]-Si(Et)Si were applied in selective conversion of fructose to 5-hydroxymethylfurfural (HMF) and 5-ethoxymethylfurfural (EMF) under microwave heating. Under the optimized conditions, the [C₄Im][OTs]-Si(Et)Si3.0 nanospheres with the largest inner diameter and the smallest shell thickness exhibit the highest HMF yield (79.4%, 15 min) in fructose dehydration. And the [C₃Im][OTf]-Si(Et)Si0.5 nanospheres with the highest acid strength possess the highest EMF yield (70.4%, 30 min) in fructose ethanolysis. The high Brønsted acid-site density and acid strength of [C_3/4Im][OTs/OTf]-Si(Et)Si catalysts accompanied by high microwave heating energy lead to excellent dehydration/ethanolysis activity. The product selectivity strongly depended on the BAILs structures and morphological characteristics of the catalyst. More importantly, the [C_3/4Im][OTs/OTf]-Si(Et)Si can be reused three times without changes in leaching of BAILs, due to strong covalent bond between BAILs and silicon/carbon framework. This work will provide a simple strategy of chemically bonded BAILs on suitable supports as efficient solid acids, and an approach of combining morphology-controlled solid acids with microwave-heating for catalytic conversion of biomass/derivatives to fuels and value-added chemicals.

This paper presents the first attempt to capture a comprehensive spatial view of land use change in the Vietnamese Mekong Delta (VMD) for a long period, i.e., from 2000 to 2020. It is aimed at monitoring holistically the land use change and flooding situation in the region, addressing the reasons for land use change, and assessing the impacts of land use change on hydraulic aspects and farmer livelihoods during the last 21 years. MODIS products, in particular, are used to study the dynamics of land use and floods after demonstrating high validation with statistical data and radar satellites, with R² = 0.96 and R² ≥ 0.97 for land use and flood maps, respectively. The results show that rice cultivation is the most dominant land use type, accounting for 40% to 46% of the delta area, while aquaculture accounts for 10% to 22%, respectively. The total rice cultivation area increased from 3764 thousand hectares (thous. ha) in 2001 to 4343 thous. ha in 2015 based on the intensive development of triple rice cropping in the upper zone, then decreased to 3963 thous. ha in 2020. In contrast, aquaculture areas are farmed mainly in the coastal area and remained relatively steady, increasing slightly from 619 thous. ha in 2001 to 856 thous. ha in 2020. The massive construction of dikes for triple rice cropping in the upper zone appears to cause a significant impact on the annual flooding regime. Land use policies have influenced the changes in land use patterns, flooding situations, and the livelihoods of local farmers.

Construction and demolition activities generate huge quantities of waste with substantial impacts on environment. This mini-review article covers the literatures relating to construction and demolition waste management practice in Australia. The Scopus search engine was used in literature search and 26 journal articles relating to construction and demolition waste management in Australia were targeted for analysis. Additionally, various government acts, regulations, policies, and strategy documents were collected and analyzed. This review indicated that the inconsistencies in legislation and landfill levies across states and territories contributed to the cross-jurisdiction waste movement. Given the stakeholders' attitude and project life cycle, this review reported that the design phase had the greatest potential to minimize waste and that the role of designers had been highlighted in various empirical studies. This review provides practitioners and academics with an understanding of the current construction and demolition waste management research in Australia and recommends directions for future research.

Exploration of the algae-derived biobutanol synthesis has become one of the hotspots due to its highly cost-effective and environment-friendly features. In this study, a solventogenic strain Clostridium sp. strain WK produced 13.96 g/L butanol with a maximal yield of 0.41 g/g from glucose in the presence of 24 g/L butyrate. Transcriptional analysis indicated that the acid re-assimilation of this strain was predominantly regulated by genes buk-ptb rather than ctfAB, explaining its special phenotypes including high butyrate tolerance and the pH-independent fermentation. In addition, a butyric acid-mediated hydrolytic system was established for the first time to release a maximal yield of 0.35 g/g reducing sugars from the red algal biomass (Gelidium amansii). Moreover, 4.48 g/L of butanol was finally achieved with a significant enhancement by 29.9 folds. This work reveals an unconventional metabolic pathway for butanol synthesis in strain WK, and demonstrates the feasibility to develop renewable biofuels from marine resources.

Six new glucosides of benzofuran (1-6), together with three known glucosides of benzofuran (8, 9, 14), nine flavonoids (12, 13, 15, 18, 19, 20, 21, 22 and 24), three coumarins (16, 17, 23) and four other-typic compounds (7, 10, 11 and 25) were isolated from the fruits of Psoralia corylifolia L. Their structures were elucidated by extensive spectroscopic methods. The biosynthesis pathway of benzofuran system was discussed. Besides, all isolated compounds and additional ring-opening derivatives of psoralen/isopsoralen (P-1, P-2, IP-1 and IP-2) were assayed for inhibition of nitric oxide (NO) production on lipopolysaccharides-induced RAW 264.7 macrophage cells. The results of the assay showed that the glycosides showed weaker or no effects, while most isolated non-glycoside compounds showed moderate or high activities. And the structure-activity relationships of non-glycoside compounds were discussed.

In parallel to the rapid growth in economic and social activities, there has been an undesirable increase in environmental degradation due to the massively produced and disposed waste. The need to manage waste in a more innovative manner has become an urgent matter. In response to the call for circular economy, some solid wastes can offer plenty of opportunities to be reutilized as raw materials for the fabrication of functional, high-value products. In the context of solid waste-derived polymeric membrane development, this strategy can pave a way to reduce the consumption of conventional feedstock for the production of synthetic polymers and simultaneously to dampen the negative environmental impacts resulting from the improper management of these solid wastes. The review aims to offer a platform for overviewing the potentials of reutilizing solid waste in liquid separation membrane fabrication by covering the important aspects, including waste pretreatment and raw material extraction, membrane fabrication and characterizations, as well as the separation performance evaluation of the resultant membranes. Three major types of waste-derived polymeric raw materials, namely keratin, cellulose, and plastics, are discussed based on the waste origins, limitations in the waste processing, and their conversion into polymeric membranes. With the promising material properties and viability of processing facilities, recycling and reutilization of waste resources for membrane fabrication are deemed to be a promising strategy that can bring about huge benefits in multiple ways, especially to make a step closer to sustainable and green membrane production.

Solventogenic clostridia has been considered as one of the most potential microbial cell factories for biofuel production in the biorefinery industry. However, the inherent shortcomings of clostridia strains such as low productivity, by-products formation and toxic tolerance still strongly restrict the large-scale application. Therefore, concerns regarding the genetic modification of solventogenic clostridia have spurred interests into the development of modern gene-editing tools. In this review, we summarize the latest advances of genetic tools involved in modifying solventogenic clostridia. Following a systematic comparison on their respective characteristics, we then review the corresponding strategies for overcoming the obstacles to the enhanced production. Discussing the progress of other microbial cell factories for solventogenesis, we finally describe the key challenges and trends with valuable recommendations for future large-scale biosolvent industrial application.