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Jiao M, Kong W, Liu W, Dong Z, Yang J, Wei Z, Lu X, Wei Y, Zhuang J. Boosting the antibacterial potency of natural products through nanotechnologies. Int J Pharm 2025; 674:125437. [PMID: 40057213 DOI: 10.1016/j.ijpharm.2025.125437] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 01/11/2025] [Revised: 03/01/2025] [Accepted: 03/06/2025] [Indexed: 03/17/2025]
Abstract
The advent of bacterial resistance has led to a notable challenge in effectively treating bacterial infections. This highlights the urgent need for the development of novel and effective drugs to combat bacterial infections. Medicinal plants, with their rich and diverse natural compounds, represent a valuable source for the discovery of novel antibacterial agents. Many of these natural compounds exhibit strong antibacterial functions, offering a promising direction for the development of antibacterial drugs. Furthermore, the application of nanotechnology in the development of antibacterial natural products has become a topic of considerable interest due to the advantages it offers, including the potential to enhance drug solubility. The efficacy of natural antibacterial agents is significantly enhanced through nanotechnology. This review offers a comprehensive overview of recent advances in the delivery of natural antibacterial compounds using a range of nanoformulation strategies.
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Affiliation(s)
- Min Jiao
- Graduate School, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China; School of Pharmacy, Shanghai University of Medicine and Health Sciences, Shanghai 201318, China
| | - Weiwen Kong
- School of Pharmacy, Fudan University, Key Laboratory of Smart Drug Delivery, Ministry of Education, Shanghai 201203, China
| | - Wenjuan Liu
- School of Pharmacy, Fudan University, Key Laboratory of Smart Drug Delivery, Ministry of Education, Shanghai 201203, China
| | - Zirong Dong
- School of Pharmacy, Fudan University, Key Laboratory of Smart Drug Delivery, Ministry of Education, Shanghai 201203, China
| | - Jinlong Yang
- School of Pharmacy, Fudan University, Key Laboratory of Smart Drug Delivery, Ministry of Education, Shanghai 201203, China; Shanghai Skin Disease Hospital, School of Medicine, Tongji University, Shanghai 200443, China
| | - Zibo Wei
- Graduate School, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China; School of Pharmacy, Shanghai University of Medicine and Health Sciences, Shanghai 201318, China
| | - Xinrui Lu
- School of Pharmacy, Fudan University, Key Laboratory of Smart Drug Delivery, Ministry of Education, Shanghai 201203, China
| | - Yuning Wei
- School of Pharmacy, Fudan University, Key Laboratory of Smart Drug Delivery, Ministry of Education, Shanghai 201203, China
| | - Jie Zhuang
- Graduate School, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China; School of Pharmacy, Shanghai University of Medicine and Health Sciences, Shanghai 201318, China.
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Tie S, Xue M, Chen Y, Wu Y, Gu S, Tan M. Enhancing stability, bioavailability and nutritional intervention effect of procyanidins using bio-based delivery systems: A review. Int J Biol Macromol 2025; 287:138517. [PMID: 39647725 DOI: 10.1016/j.ijbiomac.2024.138517] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 06/25/2024] [Revised: 11/21/2024] [Accepted: 12/05/2024] [Indexed: 12/10/2024]
Abstract
Procyanidins (PCs), a kind of polyphenolic compound, have attracted extensive attention due to their strong antioxidant, anti-inflammatory and other activities. However, PCs are susceptible to complex micro-environments, resulting in low stability, poor target tissue delivery and bioavailability, which limits their biological effects. Therefore, it is urgent to find some suitable pathways to protect PCs, avoid their degradation, and maximize their health benefits in nutritional intervention. This review focused on the design and construction of different types of bio-based delivery systems loaded with PCs, such as nanoparticles, microparticles, emulsions, liposomes, hydrogels and fibers. The advantages and biological effects of PCs-based delivery systems in promoting cellular uptake, realizing targeted release of organs, cells and organelles, and even nutritional intervention for different chronic diseases were summarized. Furthermore, the development prospects and challenges of delivery systems in the field of precision nutrition were discussed. The construction of these delivery systems can effectively improve the stability and bioavailability of PCs, and maximize their precise nutritional intervention for various chronic diseases.
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Affiliation(s)
- Shanshan Tie
- College of Food and Bioengineering, Henan University of Science and Technology, Luoyang 471023, China; Academy of Food Interdisciplinary Science, School of Food Science and Technology, Dalian Polytechnic University, Qinggongyuan1, Ganjingzi District, Dalian 116034, Liaoning, China
| | - Mengmeng Xue
- College of Food and Bioengineering, Henan University of Science and Technology, Luoyang 471023, China
| | - Yannan Chen
- College of Food Science and Engineering, Shandong Agricultural University, Taian 270018, China
| | - Ying Wu
- College of Food and Bioengineering, Henan University of Science and Technology, Luoyang 471023, China
| | - Shaobin Gu
- College of Food and Bioengineering, Henan University of Science and Technology, Luoyang 471023, China.
| | - Mingqian Tan
- Academy of Food Interdisciplinary Science, School of Food Science and Technology, Dalian Polytechnic University, Qinggongyuan1, Ganjingzi District, Dalian 116034, Liaoning, China.
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Liu K, Yu Y, Zhao H, Yang M, Zhang C, Guan F, Yao M. Cowberry extract loaded chitosan hydrogel with photothermal and antioxidant properties promotes infected wound healing. Int J Biol Macromol 2024; 262:129988. [PMID: 38325692 DOI: 10.1016/j.ijbiomac.2024.129988] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 11/20/2023] [Revised: 02/01/2024] [Accepted: 02/03/2024] [Indexed: 02/09/2024]
Abstract
Bacterial infection and oxidative stress impede clinical wound healing. Herein, the plant-derived cowberry extract (CE) was first explored as a natural photothermal agent and antioxidant to deal with bacterial infection and oxidative stress. After loading in the carboxymethyl chitosan (CMCs)/oxidized dextran (Odex) hydrogel, the photothermal effect of CE was highly enhanced by CMCs. The controlled temperature induced by CE-containing hydrogel under NIR laser irradiation could rapidly (10 min) and effectively kill Staphylococcus aureus (S. aureus, 99.3 %) and Escherichia coli (E. coli, 94.6 %). Besides, this hydrogel exhibited a fast gelation and hemostasis abilities, high stability, adhesion and ROS scavenging capabilities, as well as good injectability and biocompatibility. Above superior properties make this hydrogel to accelerate the wound healing in S. aureus-infected mice, and it is expected to be a potential clinical wound dressing.
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Affiliation(s)
- Kaiyue Liu
- School of Life Science, Zhengzhou University, 100 Science Road, Zhengzhou 450001, PR China
| | - Yachao Yu
- School of Life Science, Zhengzhou University, 100 Science Road, Zhengzhou 450001, PR China
| | - Hua Zhao
- School of Life Science, Zhengzhou University, 100 Science Road, Zhengzhou 450001, PR China
| | - Mengyu Yang
- School of Life Science, Zhengzhou University, 100 Science Road, Zhengzhou 450001, PR China
| | - Chen Zhang
- School of Life Science, Zhengzhou University, 100 Science Road, Zhengzhou 450001, PR China
| | - Fangxia Guan
- School of Life Science, Zhengzhou University, 100 Science Road, Zhengzhou 450001, PR China.
| | - Minghao Yao
- School of Life Science, Zhengzhou University, 100 Science Road, Zhengzhou 450001, PR China.
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Chen L, Hao L, Yanshuo C, FangFang W, Daqin C, Weidong X, Jian X, Shaodong C, Hongyu Z, Ke X. Grape seed proanthocyanidins regulate mitophagy of endothelial cells and promote wound healing in mice through p-JNK/FOXO3a/ROS signal pathway. Arch Biochem Biophys 2023; 749:109790. [PMID: 37858664 DOI: 10.1016/j.abb.2023.109790] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 04/06/2023] [Revised: 09/14/2023] [Accepted: 10/16/2023] [Indexed: 10/21/2023]
Abstract
Skin wound healing is a dynamic and complex process that involves multiple physiological and cellular events. Grape seed proanthocyanidins (GSP) have strong anti-oxidation and elimination of oxygen free radicals, and have been shown to significantly promote wound healing, but the underlying mechanism remains unclear. Studies have indicated that reactive oxygen species (ROS) acts as an upstream signal to induce mitophagy, suggesting that GSP can regulate mitophagy through the signal pathway. This study aimed to investigate whether GSP regulates mitophagy by down-regulating oxidative stress to promote wound healing. In vivo, GSP treatment accelerated wound healing, granulation tissue formation, collagen deposition, and angiogenesis in mice. Moreover, GSP down-regulated ROS levels and promoted the expression of antioxidant proteins by up-regulating the expression of p-JNK/FOXO3a protein, thereby regulating the expression of mitophagy-related proteins. In vitro, 4 μg/mL GSP showed no apparent toxic effects on cells and effectively reduce the oxidative stress damage of cells induced by H2O2. Western blot and superoxide anion fluorescence probe further confirmed that GSP effectively reduced Dihydroethidium content and up-regulated the expression of antioxidant proteins by activation of p-JNK/FOXO3a protein expression, thereby regulating mitophagy. Taken together, the findings from in vitro and in vivo experiments provide new insights into the promotion of wound healing by GSP.
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Affiliation(s)
- Liuqing Chen
- Institute of Life Sciences, Engineering Laboratory of Zhejiang Province for Pharmaceutical Development of Growth Factors, Biomedical Collaborative Innovation Center of Wenzhou, Wenzhou University, Zhejiang, China
| | - Li Hao
- Department of Orthopedics Surgery, Lishui People's Hospital, The Sixth Affiliated Hospital of Wenzhou Medical University, Lishui, Zhejiang, China
| | - Chen Yanshuo
- Institute of Life Sciences, Engineering Laboratory of Zhejiang Province for Pharmaceutical Development of Growth Factors, Biomedical Collaborative Innovation Center of Wenzhou, Wenzhou University, Zhejiang, China
| | - Wu FangFang
- Department of Emergency, The Second Affiliated Hospital and Yuying Children's Hospital, Wenzhou Medical University, Wenzhou, China
| | - Chen Daqin
- Department of Emergency, The Second Affiliated Hospital and Yuying Children's Hospital, Wenzhou Medical University, Wenzhou, China
| | - Xia Weidong
- Burn and Wound Healing Center, The First Affiliated Hospital of Wenzhou Medical University, Zhejiang, China
| | - Xiao Jian
- School of Pharmaceutical Sciences, Wenzhou Wound Repair and Regeneration Key Laboratory, Cixi Biomedical Research Institute, Wenzhou Medical University, Zhejiang, China
| | - Chen Shaodong
- Department of Orthopedics Surgery, Lishui People's Hospital, The Sixth Affiliated Hospital of Wenzhou Medical University, Lishui, Zhejiang, China.
| | - Zhang Hongyu
- School of Pharmaceutical Sciences, Wenzhou Wound Repair and Regeneration Key Laboratory, Cixi Biomedical Research Institute, Wenzhou Medical University, Zhejiang, China.
| | - Xu Ke
- Institute of Life Sciences, Engineering Laboratory of Zhejiang Province for Pharmaceutical Development of Growth Factors, Biomedical Collaborative Innovation Center of Wenzhou, Wenzhou University, Zhejiang, China.
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Nejaddehbashi F, Rafiee Z, Orazizadeh M, Bayati V, Hemmati A, Hashemitabar M, Makvandi P. Antibacterial and antioxidant double-layered nanofibrous mat promotes wound healing in diabetic rats. Sci Rep 2023; 13:3166. [PMID: 36823173 PMCID: PMC9950077 DOI: 10.1038/s41598-023-30240-8] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 06/10/2022] [Accepted: 02/20/2023] [Indexed: 02/25/2023] Open
Abstract
Diabetic wounds are problematic to heal owing to microbial infections as well as decreased proliferation and high concentrations of reactive oxygen species. In this study, a double-layered nanofibrous mat containing grape seed extract (GSE) and silver sulfadiazine (SSD) was fabricated. A synthetic biodegradable polymer, e.g., polycaprolactone (PCL), and a natural material (i.e., collagen) were employed as wound dressing substances. The results showed that GSE possesses antioxidant activity which can be helpful in reducing free radicals. The platform exhibited antibacterial activity against gram-positive and -negative bacteria. The double-layered nanofibrous mat containing GSE and SSD not only was not toxic but also amplified the cell proliferation compared to a pure mat, showing the effect of plant extract. After induction of a round wound, the animals were divided into three groups, namely (1) normal group (receiving + GSE/-GSE nanofiber), (2) diabetic group (receiving + GSE/-GSE nanofiber), and (3) control group (receiving gauze). In vivo evaluation demonstrated no significant differences in the healing process of normal rats. Surprisingly, fully repaired skin was observed on day 14 in the double-layered nanofibrous mat containing GSE in the normal and diabetic groups whereas the wound of diabetic rats treated with pure mat was not completely healed. The macroscopic and microscopic results after 14 days showed the following order in wound repair: Normal/ + GES > Diabetic/ + GSE > Normal/-GES > Diabetic/-GSE > control (with gauze) (p < 0.05). Accordingly, the double-layered nanofibrous mat containing GSE and SSD used in the present study could be considered as a suitable wound dressing in order to shorten healing time and prevent infection during the wound healing process.
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Affiliation(s)
- Fereshteh Nejaddehbashi
- Cellular and Molecular Research Center, Medical Basic Sciences Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
| | - Zeinab Rafiee
- grid.4514.40000 0001 0930 2361Department of Experimental Medical Science, Faculty of Medicine, Lund University, 22100 Lund, Sweden ,grid.4514.40000 0001 0930 2361Wallenberg Centre for Molecular Medicine, Lund University, 22100 Lund, Sweden
| | - Mahmoud Orazizadeh
- grid.411230.50000 0000 9296 6873Cellular and Molecular Research Center, Medical Basic Sciences Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran ,grid.411230.50000 0000 9296 6873Department of Anatomical Sciences, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
| | - Vahid Bayati
- grid.411230.50000 0000 9296 6873Cellular and Molecular Research Center, Medical Basic Sciences Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran ,grid.411230.50000 0000 9296 6873Department of Anatomical Sciences, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
| | - Aliasghar Hemmati
- grid.411230.50000 0000 9296 6873Marine Pharmaceutical Research Center, School of Pharmacy, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
| | - Mahmoud Hashemitabar
- grid.411230.50000 0000 9296 6873Cellular and Molecular Research Center, Medical Basic Sciences Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran ,grid.411230.50000 0000 9296 6873Department of Anatomical Sciences, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
| | - Pooyan Makvandi
- Centre for Materials Interfaces, Istituto Italiano di Tecnologia, Viale Rinaldo Piaggio 34, 56025, Pontedera, Pisa, Italy. .,Quzhou Affiliated Hospital of Wenzhou Medical University, Quzhou People's Hospital, Quzhou, 324000, Zhejiang, China.
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Vilkickyte G, Zilius M, Petrikaite V, Raudone L. Proanthocyanidins from Vaccinium vitis-idaea L. Leaves: Perspectives in Wound Healing and Designing for Topical Delivery. PLANTS (BASEL, SWITZERLAND) 2022; 11:2615. [PMID: 36235484 PMCID: PMC9572574 DOI: 10.3390/plants11192615] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Figures] [Subscribe] [Scholar Register] [Received: 09/21/2022] [Revised: 09/25/2022] [Accepted: 10/01/2022] [Indexed: 06/16/2023]
Abstract
The compositions and health-beneficial properties of lingonberry leaves (Vaccinium vitis-idaea L.) are well established; however, their proanthocyanidins are still heavily underutilized. Optimizing their delivery systems is key to enabling their wider applications. The present study investigates the phytochemical and 'wound-healing' properties of proanthocyanidin-rich fraction(s) (PRF) from lingonberry leaves as well as the development of optimal dermal film as a proanthocyanidin delivery system. The obtained PRF was subjected to HPLC-PDA and DMAC analyses to confirm the qualitative and quantitative profiles of different polymerization-degree proanthocyanidins. A 'wound healing' in vitro assay was performed to assess the ability of PRF to modulate the wound environment for better healing. Low concentrations of lingonberry proanthocyanidins were found to accelerate 'wound' closures, while high levels inhibited human fibroblast migration. Fifteen dermal films containing PRF were prepared and evaluated based on their polymer (MC, HEC, PEG 400) compositions, and physical, mechanical, and biopharmaceutical properties using an experimental design. The composition containing 0.30 g of MC, 0.05 g of HEC, and 3.0 g of PEG 400 was selected as a promising formulation for PRF delivery and a potentially effective functional wound dressing material, supporting the need for further investigations.
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Affiliation(s)
- Gabriele Vilkickyte
- Laboratory of Biopharmaceutical Research, Institute of Pharmaceutical Technologies, Lithuanian University of Health Sciences, Sukileliu Av. 13, LT-50162 Kaunas, Lithuania
| | - Modestas Zilius
- Laboratory of Pharmaceutical Sciences, Institute of Pharmaceutical Technologies, Lithuanian University of Health Sciences, Sukileliu Av. 13, LT-50162 Kaunas, Lithuania
- Department of Clinical Pharmacy, Lithuanian University of Health Sciences, Sukileliu Av. 13, LT-50162 Kaunas, Lithuania
| | - Vilma Petrikaite
- Laboratory of Drug Targets Histopathology, Institute of Cardiology, Lithuanian University of Health Sciences, Sukileliu Av. 13, LT-50162 Kaunas, Lithuania
| | - Lina Raudone
- Laboratory of Biopharmaceutical Research, Institute of Pharmaceutical Technologies, Lithuanian University of Health Sciences, Sukileliu Av. 13, LT-50162 Kaunas, Lithuania
- Department of Pharmacognosy, Lithuanian University of Health Sciences, Sukileliu Av. 13, LT-50162 Kaunas, Lithuania
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7
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Biocompatible Films of Collagen-Procyanidin for Wound Healing Applications. Appl Biochem Biotechnol 2022; 194:4002-4017. [PMID: 35579739 DOI: 10.1007/s12010-022-03956-y] [Citation(s) in RCA: 5] [Impact Index Per Article: 1.7] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 02/09/2022] [Accepted: 05/02/2022] [Indexed: 11/02/2022]
Abstract
The study investigated the effect of polyphenols present in Cassia auriculata (CA) leaves in enhancing the stability of the collagen protein and the wound healing potential of collagen films. The crude ethanol extract of CA was analyzed for the presence of phytochemicals and purified by column chromatography using solvents with increasing polarity. The ethanol eluted active fractions (EEAF) that precipitated gelatin was characterized using HP-TLC, FTIR spectroscopy, ESI-FT-MS/MS, and 1H NMR spectroscopy. The active compound was identified to be procyanidin B belonging to the proanthocyanidins group. The wound healing property of EEAF and collagen type I extracted from Clarias batrachus fish skin and the bovine tendon was assessed by in vitro scratch assay on L929 mice fibroblast cell lines. The EEAF-treated collagen coating enhanced in vitro wound closure in comparison with the uncoated dish. It was observed that EEAF treatment improved the physical strength of collagen films. The in vivo wound healing of the EEAF-treated collagen film was examined in male Wister rats and the wound site tissues were assessed. In vivo wound examination showed enhanced healing with EEAF incorporated collagen films. Comparatively, the EEAF-treated bovine tendon collagen films showed improved physical properties and better wound healing property than fish collagen films.
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Lv Y, Xu Y, Sang X, Li C, Liu Y, Guo Q, Ramakrishna S, Wang C, Hu P, Nanda HS. PLLA-gelatin composite fiber membranes incorporated with functionalized CeNPs as a sustainable wound dressing substitute promoting skin regeneration and scar remodelling. J Mater Chem B 2022; 10:1116-1127. [DOI: 10.1039/d1tb02677a] [Citation(s) in RCA: 6] [Impact Index Per Article: 2.0] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/21/2022]
Abstract
The need of wound dressing material that can accelerate wound healing is increasing and will last a long time. In this study, Cerium Oxide Nanoparticles (CeNPs) incorporated poly-L-lactic acid (PLLA)-gelatin...
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Al-Mogbel MS, Elabbasy MT, Menazea AA, Sadek AW, Ahmed MK, Abd El-Kader MFH. Conditions adjustment of polycaprolactone nanofibers scaffolds encapsulated with core shells of Au@Se via laser ablation for wound healing applications. SPECTROCHIMICA ACTA. PART A, MOLECULAR AND BIOMOLECULAR SPECTROSCOPY 2021; 259:119899. [PMID: 33992892 DOI: 10.1016/j.saa.2021.119899] [Citation(s) in RCA: 10] [Impact Index Per Article: 2.5] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 02/15/2021] [Revised: 04/24/2021] [Accepted: 04/30/2021] [Indexed: 05/24/2023]
Abstract
Au@Se core-shell nanoparticles were obtained via laser ablation technique to be incorporated into polycaprolactone (PCL) nanofibrous scaffolds for wound healing applications at different contributions of Se nanoparticles (SeNPs). The synthesized layers were inspected via X-ray diffraction (XRD) and Fourier transformed infrared (FTIR). Additionally, microstructural and surface morphology were followed with different SeNPs contributions before and after fibroblast culturing. Moreover, Selenium dopant is affected Maximum roughness valley depth while it starts from 0.31 µm at Au@0.0Se@PCL reaching 0.457 µm at Au@12Se@PCL; however, after culturing starts from 0.3833 µm reaching 0.41 µm. Besides, the antibacterial activity was screened, showing the absence of inhibition zones in free selenium composition; however, it grows up reaching 8.3 ± 0.8, and 8.0 ± 0.8 for E. coli and S. aureus, respectively at the maximum contribution of selenium. SeNPs contributed composites show higher cell viability than Selenium free composite that it reaches its max in Au@8.0Se@PCL, recording 95.3 ± 2.3%. Composites show an excellent Wound dressing capability that its performance is directly proportional to selenium content. This significant enrichment of antibacterial activity and cell viability could recommend these composites for additional research in medical applications.
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Affiliation(s)
- Mohammed S Al-Mogbel
- Medical Laboratory Sciences Department, College of Applied Medical Sciences, Ha'il University, Ha'il, Saudi Arabia
| | - M T Elabbasy
- Public Health Department, College of Public Health and Health Informatics, Ha'il University, Ha'il, Saudi Arabia; Food Control Department, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt
| | - A A Menazea
- Laser Technology Unit, Physics Division, National Research Centre, Dokki, Giza, Egypt; Spectroscopy Department, National Research Centre, Dokki, Giza, Egypt.
| | - A W Sadek
- Biophysics Department, Faculty of Women for Arts, Science and Education, Ain Shams University, Cairo, Egypt
| | - M K Ahmed
- Faculty of Nanotechnology for Postgraduate Studies, Cairo University, El-Sheikh Zayed 12588, Egypt; Department of Physics, Faculty of Science, Suez University, Suez, 43518, Egypt.
| | - M F H Abd El-Kader
- Biophysics Department, Faculty of Science, Cairo University, Giza, Egypt; Basic Sciences Department, Deanship of Preparatory Year, Ha'il University, Ha'il, Saudi Arabia
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10
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Tan E, Kahyaoğlu İM, Karakuş S. A sensitive and smartphone colorimetric assay for the detection of hydrogen peroxide based on antibacterial and antifungal matcha extract silver nanoparticles enriched with polyphenol. Polym Bull (Berl) 2021; 79:7363-7389. [PMID: 34413556 PMCID: PMC8364309 DOI: 10.1007/s00289-021-03857-w] [Citation(s) in RCA: 10] [Impact Index Per Article: 2.5] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 07/07/2021] [Revised: 08/02/2021] [Accepted: 08/09/2021] [Indexed: 12/16/2022]
Abstract
Current trends in scientific studies focus on the development of smartphone-based biosensors via green nanoparticle for clinical diagnosis, food, and environmental monitoring. In this study, we developed a novel portable smartphone-based biosensor via green dendrimer-coated matcha extract/silver nanoparticles (ME-Ag NPs) enriched with polyphenol for detecting hydrogen peroxide (H2O2). Also, we investigated the biological evaluation of the nanostructure as a safe preservative for use in biomedical applications. Ag NPs were prepared using a green sonochemical method and were characterized to determine surface and chemical properties by different techniques such as scanning electron microscopy-energy-dispersive X-ray, transmission electron microscope, Fourier transform infrared spectroscopy, atomic force microscopy, X-ray diffraction, and Brunauer-Emmett-Teller. Furthermore, antimicrobial and antifungal properties of ME-Ag NPs were investigated against pathogenic microorganisms such as Staphylococcus aureus, Pseudomonas aureginosa, Escherichia coli, Candida albicans, and Aspergillus brasiliensis. The experimental sensor methodology was based on the detection of H2O2 by analysis of images of novel silver nanostructure-coated papers and processing of color histograms with a RGB (red-green-blue) analyzer software. Consequently, the smartphone-based biosensor exhibited high sensitivity with detection limits of 0.82 μM response time of 5 s. The smartphone-based biosensor via ME-Ag NPs provided a rapid and selective detection of H2O2.
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Affiliation(s)
- Ezgi Tan
- Department of Chemistry, Faculty of Engineering, Istanbul University-Cerrahpasa, Avcilar, 34320 Istanbul, Turkey
| | - İbrahim Mizan Kahyaoğlu
- Department of Chemistry, Faculty of Science and Arts, Kurupelit, Ondokuz Mayis University, 55139 Samsun, Turkey
| | - Selcan Karakuş
- Department of Chemistry, Faculty of Engineering, Istanbul University-Cerrahpasa, Avcilar, 34320 Istanbul, Turkey
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11
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El-Hamshary H, El-Naggar ME, El-Faham A, Abu-Saied MA, Ahmed MK, Al-Sahly M. Preparation and Characterization of Nanofibrous Scaffolds of Ag/Vanadate Hydroxyapatite Encapsulated into Polycaprolactone: Morphology, Mechanical, and In Vitro Cells Adhesion. Polymers (Basel) 2021; 13:1327. [PMID: 33919554 PMCID: PMC8073657 DOI: 10.3390/polym13081327] [Citation(s) in RCA: 13] [Impact Index Per Article: 3.3] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 03/25/2021] [Revised: 04/12/2021] [Accepted: 04/13/2021] [Indexed: 02/06/2023] Open
Abstract
Series of nanofibrous composites of polycaprolactone (PCL) were fabricated in different compositions of modified hydroxyapatite (HAP). The encapsulated HAP was co-doped with Ag/vanadate ions at different Ag contributions. XRD and FTIR techniques confirmed the powder and fibrous phase formation. Further, the morphological and mechanical behaviors of the electrospun nanofibrous scaffolds containing hydroxyapatite were investigated. The nanofibrous phases were biologically evaluated via studying contact angle, antibacterial, cell viability, and in vitro growth of human fibroblasts cell line (HFB4). It is obvious that silver ions cause gradual deviation in powder grains from wafer-like to cloudy grains. The maximum height of the roughness (Rt) ranged from 902.0 to 956.9 nm, while the valley depth of the roughness (Rv) ranged from 308.3 to 442.8 nm, for the lowest and the highest additional Ag ions for powdered phases. Moreover, the highest contribution of silver through the nanofibrous phases leads to the formation of lowest filaments size ranged from 0.07 to 0.53 µm. Further, the fracture strength was increased exponentially from 2.51 ± 0.35 MPa at zero concentration of silver ions up to 4.23 ± 0.64 MPa at 0.6 Ag/V-HAP@PCL. The fibrous phases were biologically evaluated in terms of antibacterial, cell viability, and in vitro growth of human fibroblasts cell line (HFB4). The nanofibrous composition of 0.8 Ag/V-HAP@PCL reached the maximum potential against E. coli and S. aureus and recorded 20.3 ± 1.1 and 19.8 ± 1.2 mm, respectively. This significant performance of the antibacterial activity and cell viability of co-doped HAP distributed through PCL could recommend these compositions for more research in biological applications, including wound healing.
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Affiliation(s)
- Hany El-Hamshary
- Chemistry Department, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia; (A.E.-F.); (M.A.-S.)
- Department of Chemistry, Faculty of Science, Tanta University, Tanta 31527, Egypt
| | - Mehrez E. El-Naggar
- Textile Research Division, National Research Centre, Dokki, Cairo 12622, Egypt
| | - Ayman El-Faham
- Chemistry Department, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia; (A.E.-F.); (M.A.-S.)
- Department of Chemistry, Faculty of Science, Alexandria University, P.O. Box 426, Ibrahimia, Alexandria 21321, Egypt
| | - M. A. Abu-Saied
- Polymeric Materials Research Department, Advanced Technology and New Materials Research Institute, City of Scientific Research and Technological Applications (SRTA-CITY), New Borg El-Arab City 21934, Alexandria, Egypt;
| | - M. K. Ahmed
- Department of Physics, Faculty of Science, Suez University, Suez 43518, Egypt;
- Faculty of Nanotechnology for Postgraduate Studies, Cairo University, El-Sheikh Zayed 12588, Egypt
| | - Mosaed Al-Sahly
- Chemistry Department, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia; (A.E.-F.); (M.A.-S.)
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12
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Gubitosa J, Rizzi V, Fini P, Laurenzana A, Fibbi G, Veiga-Villauriz C, Fanelli F, Fracassi F, Onzo A, Bianco G, Gaeta C, Guerrieri A, Cosma P. Biomolecules from snail mucus (Helix aspersa) conjugated gold nanoparticles, exhibiting potential wound healing and anti-inflammatory activity. SOFT MATTER 2020; 16:10876-10888. [PMID: 33225330 DOI: 10.1039/d0sm01638a] [Citation(s) in RCA: 32] [Impact Index Per Article: 6.4] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 06/11/2023]
Abstract
In this work, for the first time, snail slime from garden snails "Helix Aspersa Müller", has been used to induce the formation of eco-friendly gold nanoparticles (AuNPs-SS) suitable for biomedical applications. An AuNPs-SS comprehensive investigation was performed and AuNPs with an average particle size of 14 ± 6 nm were observed, stabilized by a slime snail-based organic layer. Indeed, as recognized in high-resolution MALDI-MS analyses, and corroborated by FESEM, UV-Vis, ATR-FTIR, and XPS results, it was possible to assess the main presence of peptides and amino acids as the main components of the slime, that, combined with the AuNPs confers on them interesting properties. More specifically, we tested, in vitro, the AuNPs-SS safety in human keratinocytes and their potential effect on wound healing as well as their anti-inflammatory properties in murine macrophages. Moreover, the AuNPs-SS treatment resulted in a significant increase of the urokinase-type plasminogen activator receptor (uPAR), essential for keratinocyte adhesion, spreading, and migration, together with the reduction of LPS-induced IL1-β and IL-6 cytokine levels, and completely abrogated the synthesis of inducible nitric oxide synthase (iNOS).
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Affiliation(s)
- Jennifer Gubitosa
- Università degli Studi "Aldo Moro" di Bari, Dip. Chimica, Via Orabona, 4 - 70126 Bari, Italy.
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13
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Nour S, Imani R, Chaudhry GR, Sharifi AM. Skin wound healing assisted by angiogenic targeted tissue engineering: A comprehensive review of bioengineered approaches. J Biomed Mater Res A 2020; 109:453-478. [PMID: 32985051 DOI: 10.1002/jbm.a.37105] [Citation(s) in RCA: 45] [Impact Index Per Article: 9.0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 06/10/2020] [Revised: 09/23/2020] [Accepted: 09/26/2020] [Indexed: 12/16/2022]
Abstract
Skin injuries and in particular, chronic wounds, are one of the major prevalent medical problems, worldwide. Due to the pivotal role of angiogenesis in tissue regeneration, impaired angiogenesis can cause several complications during the wound healing process and skin regeneration. Therefore, induction or promotion of angiogenesis can be considered as a promising approach to accelerate wound healing. This article presents a comprehensive overview of current and emerging angiogenesis induction methods applied in several studies for skin regeneration, which are classified into the cell, growth factor, scaffold, and biological/chemical compound-based strategies. In addition, the advantages and disadvantages of these angiogenic strategies along with related research examples are discussed in order to demonstrate their potential in the treatment of wounds.
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Affiliation(s)
- Shirin Nour
- Department of Biomedical Engineering, Amirkabir University of Technology (Tehran Polytechnic), Tehran, Iran
| | - Rana Imani
- Department of Biomedical Engineering, Amirkabir University of Technology (Tehran Polytechnic), Tehran, Iran
| | - G Rasul Chaudhry
- OU-WB Institute for Stem Cell and Regenerative Medicine, Department of Biological Sciences, Oakland University, Rochester, Michigan, USA
| | - Ali Mohammad Sharifi
- Stem Cell and Regenerative Medicine Research Center, Iran University of Medical Sciences, Tehran, Iran.,Tissue Engineering Group (NOCERAL), Department of Orthopedics Surgery, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.,Department of Tissue Engineering and Regenerative Medicine, School of Advanced Technologies in Medicine, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
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14
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Salazar-López NJ, Domínguez-Avila JA, Yahia EM, Belmonte-Herrera BH, Wall-Medrano A, Montalvo-González E, González-Aguilar GA. Avocado fruit and by-products as potential sources of bioactive compounds. Food Res Int 2020; 138:109774. [PMID: 33292952 DOI: 10.1016/j.foodres.2020.109774] [Citation(s) in RCA: 53] [Impact Index Per Article: 10.6] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 05/28/2020] [Revised: 09/23/2020] [Accepted: 09/27/2020] [Indexed: 12/22/2022]
Abstract
The increased demand for avocado, and therefore production and consumption, generate large quantities of by-products such as seeds, peel, and defatted pulp, which account for approximately 30% of fruit weight, and which are commonly discarded and wasted. The present review focuses on various compounds present in avocado fruit and its by-products, with particular interest to those that can be potentially used in different industrial forms, such as nutraceuticals, to add to or to formulate functional foods, among other uses. Main molecular families of bioactive compounds present in avocado include phenolic compounds (such as hydroxycinnamic acids, hydroxybenzoic acids, flavonoids and proanthocyanins), acetogenins, phytosterols, carotenoids and alkaloids. Types, contents, and possible functions of these bioactive compounds are described from a chemical, biological, and functional approach. The use of avocado and its by-products requires using processing methods that allow highest yield with the least amount of unusable residues, while also preserving the integrity of bioactive compounds of interest. Avocado cultivar, fruit development, ripening stage, and processing methods are some of the main factors that influence the type and amount of extractable molecules. The phytochemical diversity of avocado fruit and its by-products make them potential sources of nutraceutical compounds, from which functional foods can be obtained, as well as other applications in food, health, pigment, and material sectors, among others.
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Affiliation(s)
- Norma Julieta Salazar-López
- Centro de Investigación en Alimentación y Desarrollo A. C., Carretera Gustavo Enrique Astiazarán Rosas No. 46, Col. La Victoria, Hermosillo, Sonora 83304, Mexico
| | - J Abraham Domínguez-Avila
- Cátedras CONACYT-Centro de Investigación en Alimentación y Desarrollo A. C., Carretera Gustavo Enrique Astiazarán Rosas No. 46, Col. La Victoria, Hermosillo, Sonora 83304, Mexico
| | - Elhadi M Yahia
- Laboratorio de Fitoquímicos y Nutrición, Facultad de Ciencias Naturales, Universidad Autónoma de Querétaro, Avenida de las Ciencias, Juriquilla, Querétaro, 76230 Qro., Mexico.
| | - Beatriz Haydee Belmonte-Herrera
- Centro de Investigación en Alimentación y Desarrollo A. C., Carretera Gustavo Enrique Astiazarán Rosas No. 46, Col. La Victoria, Hermosillo, Sonora 83304, Mexico
| | - Abraham Wall-Medrano
- Instituto de Ciencias Biomédicas, Universidad Autónoma de Ciudad Juárez, Anillo Envolvente del PRONAF y Estocolmo s/n, Ciudad Juárez, Chihuahua 32310, Mexico.
| | - Efigenia Montalvo-González
- Laboratorio Integral de Investigación en Alimentos, Tecnológico Nacional de México-Instituto Tecnológico de Tepic, Av. Tecnológico 255 Fracc. Lagos del Country, Tepic, Nayarit 63175, Mexico.
| | - G A González-Aguilar
- Centro de Investigación en Alimentación y Desarrollo A. C., Carretera Gustavo Enrique Astiazarán Rosas No. 46, Col. La Victoria, Hermosillo, Sonora 83304, Mexico.
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15
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Koppa Raghu P, Bansal KK, Thakor P, Bhavana V, Madan J, Rosenholm JM, Mehra NK. Evolution of Nanotechnology in Delivering Drugs to Eyes, Skin and Wounds via Topical Route. Pharmaceuticals (Basel) 2020; 13:E167. [PMID: 32726897 PMCID: PMC7463474 DOI: 10.3390/ph13080167] [Citation(s) in RCA: 25] [Impact Index Per Article: 5.0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 06/30/2020] [Revised: 07/21/2020] [Accepted: 07/21/2020] [Indexed: 12/23/2022] Open
Abstract
The topical route is the most preferred one for administering drugs to eyes, skin and wounds for reaching enhanced efficacy and to improve patient compliance. Topical administration of drugs via conventional dosage forms such as solutions, creams and so forth to the eyes is associated with very low bioavailability (less than 5%) and hence, we cannot rely on these for delivering drugs to eyes more efficiently. An intravitreal injection is another popular drug delivery regime but is associated with complications like intravitreal hemorrhage, retinal detachment, endophthalmitis, and cataracts. The skin has a complex structure that serves as numerous physiological barriers to the entry of exogenous substances. Drug localization is an important aspect of some dermal diseases and requires directed delivery of the active substance to the diseased cells, which is challenging with current approaches. Existing therapies used for wound healing are costly, and they involve long-lasting treatments with 70% chance of recurrence of ulcers. Nanotechnology is a novel and highly potential technology for designing formulations that would improve the efficiency of delivering drugs via the topical route. This review involves a discussion about how nanotechnology-driven drug delivery systems have evolved, and their potential in overcoming the natural barriers for delivering drugs to eyes, skin and wounds.
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Affiliation(s)
- Pratheeksha Koppa Raghu
- Pharmaceutical Nanotechnology Research Laboratory, Department of Pharmaceutics, National Institute of Pharmaceutical Education and Research, Hyderabad 500037, Telangana, India; (P.K.R.); (P.T.); (V.B.); (J.M.)
| | - Kuldeep K. Bansal
- Pharmaceutical Sciences Laboratory, Faculty of Science and Engineering, Åbo Akademi University, 20520 Turku, Finland;
| | - Pradip Thakor
- Pharmaceutical Nanotechnology Research Laboratory, Department of Pharmaceutics, National Institute of Pharmaceutical Education and Research, Hyderabad 500037, Telangana, India; (P.K.R.); (P.T.); (V.B.); (J.M.)
| | - Valamla Bhavana
- Pharmaceutical Nanotechnology Research Laboratory, Department of Pharmaceutics, National Institute of Pharmaceutical Education and Research, Hyderabad 500037, Telangana, India; (P.K.R.); (P.T.); (V.B.); (J.M.)
| | - Jitender Madan
- Pharmaceutical Nanotechnology Research Laboratory, Department of Pharmaceutics, National Institute of Pharmaceutical Education and Research, Hyderabad 500037, Telangana, India; (P.K.R.); (P.T.); (V.B.); (J.M.)
| | - Jessica M. Rosenholm
- Pharmaceutical Sciences Laboratory, Faculty of Science and Engineering, Åbo Akademi University, 20520 Turku, Finland;
| | - Neelesh Kumar Mehra
- Pharmaceutical Nanotechnology Research Laboratory, Department of Pharmaceutics, National Institute of Pharmaceutical Education and Research, Hyderabad 500037, Telangana, India; (P.K.R.); (P.T.); (V.B.); (J.M.)
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16
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Rajakumari R, Volova T, Oluwafemi OS, Rajesh Kumar S, Thomas S, Kalarikkal N. Grape seed extract-soluplus dispersion and its antioxidant activity. Drug Dev Ind Pharm 2020; 46:1219-1229. [PMID: 32643446 DOI: 10.1080/03639045.2020.1788059] [Citation(s) in RCA: 13] [Impact Index Per Article: 2.6] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 10/23/2022]
Abstract
OBJECTIVE The main objective of this work was to formulate a nanodispersion containing grape seed extract and analyzed its release profile, antioxidant potential of the prepared formulations. METHODS The grape seed extract (GSE) containing proanthocyanidins (PC's) has been dispersed in polymer matrix soluplus (SOLU) by the freeze-drying method. The morphological analysis was carried out using atomic force microscopy (AFM), scanning electron microscopy (SEM) and Transmission electron microscopy (TEM). The in-vitro release of the nanodispersion formulations was evaluated by simulated intestinal fluid (SIF). The antioxidant activity of GSE and the formulation were evaluated by employing various in-vitro assays such as 2, 2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS), 2, 2-diphenyl-1- picrylhydrazyl (DPPH), Ferric reducing antioxidant power (FRAP) and peroxidation inhibiting activity. RESULTS The formulation FIII (1:5) resulted in a stable formulation with a higher loading efficiency of 95.36%, a particle size of 69.90 nm, a polydispersity index of 0.154 and a zeta potential value of -82.10 mV. The antioxidant efficiency of GSE-SOLU evaluated by DPPH was found to be 96.7%. The ABTS and FRAP model exhibited a dose-dependent scavenging activity. Linoleic model of FIII formulation and GSE exhibited a 66.14 and 86.58% inhibition respectively at 200 µg/l. CONCLUSIONS The main reason for excellent scavenging activity of the formulations can be attributed to the presence of monomeric, dimeric, oligomeric procyanidins and the phenolic group. The present work denotes that GSE constitutes a good source of PC's and will be useful in the prevention and treatment of free radical related diseases.
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Affiliation(s)
- R Rajakumari
- International and Inter-University Centre for Nanoscience and Nanotechnology, Mahatma Gandhi University, Kottayam, India.,Institute of Biophysics, Siberian Federal University, Krasnoyarsk, Russia
| | - Tatiana Volova
- Institute of Biophysics, Siberian Federal University, Krasnoyarsk, Russia
| | - Oluwatobi Samuel Oluwafemi
- Department of Chemical Sciences, University of Johannesburg, Johannesburg, South Africa.,Centre for Nanomaterials Sciences Research, University of Johannesburg, Johannesburg, South Africa
| | - S Rajesh Kumar
- Department of Pharmacology, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences (SIMATS), Chennai, India
| | - Sabu Thomas
- International and Inter-University Centre for Nanoscience and Nanotechnology, Mahatma Gandhi University, Kottayam, India.,School of Chemical Sciences, Mahatma Gandhi University, Kottayam, India
| | - Nandakumar Kalarikkal
- International and Inter-University Centre for Nanoscience and Nanotechnology, Mahatma Gandhi University, Kottayam, India.,School of Pure and Applied Physics, Mahatma Gandhi University, Kottayam, India
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17
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Afifi M, Ahmed MK, Fathi AM, Uskoković V. Physical, electrochemical and biological evaluations of spin-coated ε-polycaprolactone thin films containing alumina/graphene/carbonated hydroxyapatite/titania for tissue engineering applications. Int J Pharm 2020; 585:119502. [PMID: 32505577 DOI: 10.1016/j.ijpharm.2020.119502] [Citation(s) in RCA: 17] [Impact Index Per Article: 3.4] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 04/06/2020] [Revised: 05/28/2020] [Accepted: 05/31/2020] [Indexed: 02/06/2023]
Abstract
Composite structures are at the frontier of materials science and engineering and polymeric/ceramic composites present one of their most prospective subsets. Prior studies have shown both improvements and deteriorations of properties of polymers upon the addition of ceramic phases to them, but not many studies have dealt with the direct comparison of chemically distinct inorganic additives. The goal of this study was to compare the properties of ε-polycaprolactone (PCL) thin films supplemented with alumina, graphene, carbonated hydroxyapatite or titania particles, individually, in identical amounts (12 wt%). The composite films were analyzed for their phase composition, grain size, morphology, surface roughness, porosity, cell response, mechanical properties and electrochemical performance. Each additive imparted one or more physical or biological properties onto PCL better than others. Thus, alumina increased the microhardness of the films better than any other additive, with the resulting values exceeding 10 MPa. It also led to the formation of a composite with the least porosity and the greatest stability to degradation in simulated body fluid based on open circuit potential (OCP) measurements and electrochemical impedance spectroscopy (EIS). Titania made the surface of PCL roughest, which in combination with its high porosity explained why it was the most conducive to the growth of human fibroblasts, alongside being most prone to degradation in wet, corrosive environments and having the highest Poisson's ratio. Graphene, in contrast, made the surface of PCL smoothest and the bulk structure most porous, but also most conductive, with the OCP of -37 mV. The OCP of PCL supplemented with carbonated hydroxyapatite had the highest OCP of -134 mV and also the highest mechanical moduli, including the longitudinal (781 MPa), the shear (106 MPa), the bulk (639 MPa), and the elastic (300 MPa). The only benefit of the deposition of multilayered PCL films supplemented with all four inorganic additives was to enable a relatively high resistance to degradation. This study demonstrates that the properties of thin PCL films could be effectively optimized through the simple choice of appropriate inorganic additives dispersed in them. There is no single additive that proves ideal for improving all the properties of interest in PCL thin films, but their choice should be adjusted to the actual application. One such method of compositional optimization could prove crucial in the effort to develop biocomposites for superior performance in tissue engineering applications.
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Affiliation(s)
- M Afifi
- Ultrasonic Laboratory, National Institute of Standards, Giza, Egypt.
| | - M K Ahmed
- Department of Physics, Faculty of Science, Suez University, Suez, Egypt.
| | - A M Fathi
- Physical Chemistry Department, National Research Centre, Dokki, Giza 12622, Egypt
| | - Vuk Uskoković
- Department of Mechanical and Aerospace Engineering, University of California, Irvine, USA
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18
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Gomathi M, Prakasam A, Rajkumar P, Rajeshkumar S, Chandrasekaran R, Anbarasan P. Green synthesis of silver nanoparticles using Gymnema sylvestre leaf extract and evaluation of its antibacterial activity. SOUTH AFRICAN JOURNAL OF CHEMICAL ENGINEERING 2020. [DOI: 10.1016/j.sajce.2019.11.005] [Citation(s) in RCA: 6] [Impact Index Per Article: 1.2] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Indexed: 10/25/2022] Open
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