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Qin ZN, Chen JM, Li JW, Zhen ZP, Chen QZ, Zhang ZQ, Wang GH, Gao YF. Rapid absolute quantification of glucose and fructose isomers in honey using a boronic acid-based reactive matrix by MALDI-TOF/TOF tandem mass spectrometry. Food Chem 2025; 477:143623. [PMID: 40023024 DOI: 10.1016/j.foodchem.2025.143623] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 11/19/2024] [Revised: 01/22/2025] [Accepted: 02/24/2025] [Indexed: 03/04/2025]
Abstract
The slight structural differences between carbohydrate isomers pose considerable difficulties for their identification and quantification. Herein, a rapid method for the absolute quantification of glucose and fructose isomers using matrix-assisted laser desorption/ionization time-of-flight/time-of-flight tandem mass spectrometry (MALDI-TOF/TOF MS/MS). A novel reagent, 4-trimethylamino-6-(4-methoxy-1-naphthyl)-1,3,5-triazine-2-(3-aminophenylboronic acid) (TMNTA), was synthesized and employed as a reactive matrix. This matrix significantly enhances the ionization efficiency of monosaccharides while avoiding matrix interferences. Glucose-13C6 was utilized as an internal standard for the quantitative analysis of both monosaccharides, exhibiting a strong linear correlation (R2 ≥ 0.990). By integrating the concentration relationships of glucose and fructose derived from two diagnostic ions produced from the TMNTA-derivatized monosaccharides, this approach facilitates the absolute quantification of glucose and fructose. The method was successfully applied to the absolute quantification of these monosaccharides in honey. The proposed approach offers a simple, rapid, and high-throughput analysis of glucose and fructose isomers in complex samples.
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Affiliation(s)
- Zhang-Na Qin
- Institute of Biological and Medical Engineering, Guangdong Academy of Science, Guangzhou 510316, PR China; Research Center for Sugarcane Industry Engineering Technology of Light Industry of China, Guangzhou 510316, PR China
| | - Jia-Min Chen
- Institute of Biological and Medical Engineering, Guangdong Academy of Science, Guangzhou 510316, PR China; Research Center for Sugarcane Industry Engineering Technology of Light Industry of China, Guangzhou 510316, PR China
| | - Jia-Wei Li
- Institute of Biological and Medical Engineering, Guangdong Academy of Science, Guangzhou 510316, PR China; Research Center for Sugarcane Industry Engineering Technology of Light Industry of China, Guangzhou 510316, PR China
| | - Zhen-Peng Zhen
- Institute of Biological and Medical Engineering, Guangdong Academy of Science, Guangzhou 510316, PR China; Research Center for Sugarcane Industry Engineering Technology of Light Industry of China, Guangzhou 510316, PR China
| | - Qi-Zhao Chen
- Institute of Biological and Medical Engineering, Guangdong Academy of Science, Guangzhou 510316, PR China; Research Center for Sugarcane Industry Engineering Technology of Light Industry of China, Guangzhou 510316, PR China
| | - Zhi-Qiang Zhang
- Institute of Biological and Medical Engineering, Guangdong Academy of Science, Guangzhou 510316, PR China; Research Center for Sugarcane Industry Engineering Technology of Light Industry of China, Guangzhou 510316, PR China
| | - Gui-Hua Wang
- Institute of Biological and Medical Engineering, Guangdong Academy of Science, Guangzhou 510316, PR China; Research Center for Sugarcane Industry Engineering Technology of Light Industry of China, Guangzhou 510316, PR China
| | - Yu-Feng Gao
- Institute of Biological and Medical Engineering, Guangdong Academy of Science, Guangzhou 510316, PR China; Research Center for Sugarcane Industry Engineering Technology of Light Industry of China, Guangzhou 510316, PR China.
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Gao H, Fang Q, Bai Y, Hu C, Chou HH. Biosynthesis of 12-aminododecanoic acid from biomass sugars. Metab Eng 2025; 89:87-96. [PMID: 39999943 DOI: 10.1016/j.ymben.2025.02.010] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 11/13/2024] [Revised: 01/21/2025] [Accepted: 02/20/2025] [Indexed: 02/27/2025]
Abstract
Biosynthesis of 12-aminododecanoic acid (ADDA) directly from biomass-derived sugars would enable a more sustainable process for manufacturing the engineering polymer Nylon 12. ADDA biosynthesis is currently hindered by the cytotoxicity of dodecanoic acid (DDA) to growing cells, and the accumulation of the overoxidized byproduct dodecanedioic acid (DDDA). In this study, these challenges were addressed by engineering an autoinducible system to better control in vivo DDA synthesis without impacting growth, and deleting aldehyde dehydrogenases and oxidases to reduce DDDA accumulation. As a result, a one-step fermentation process was established to synthesize ADDA from glucose and cellobiose. Finally, batch fermentation achieved 1035 mg/L ADDA and 5% yield, which is the highest titer and yield accomplished directly from sugar to date. This research contributes to the mechanistic understanding of microbial DDA, ADDA, and DDDA synthesis, as well as the goal of developing more sustainable processes for nylon production.
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Affiliation(s)
- Haixin Gao
- CAS Key Laboratory of Quantitative Engineering Biology, Center for Synthetic Biochemistry, Shenzhen Institute of Synthetic Biology, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, 518055, China
| | - Qiang Fang
- CAS Key Laboratory of Quantitative Engineering Biology, Center for Synthetic Biochemistry, Shenzhen Institute of Synthetic Biology, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, 518055, China
| | - Yanfen Bai
- CAS Key Laboratory of Quantitative Engineering Biology, Center for Synthetic Biochemistry, Shenzhen Institute of Synthetic Biology, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, 518055, China
| | - Chunyue Hu
- CAS Key Laboratory of Quantitative Engineering Biology, Center for Synthetic Biochemistry, Shenzhen Institute of Synthetic Biology, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, 518055, China
| | - Howard H Chou
- CAS Key Laboratory of Quantitative Engineering Biology, Center for Synthetic Biochemistry, Shenzhen Institute of Synthetic Biology, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, 518055, China.
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Yao B, Gu L, Huang L, Li R, Fan Z, Chen Z, Qin D, Gao L. Using Magnetic Molecularly Imprinted Polymer Technology for Determination of Fish Serum Glucose Levels. Polymers (Basel) 2024; 16:1538. [PMID: 38891484 PMCID: PMC11174484 DOI: 10.3390/polym16111538] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Grants] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 03/25/2024] [Revised: 05/20/2024] [Accepted: 05/23/2024] [Indexed: 06/21/2024] Open
Abstract
In this study, a highly efficient magnetic molecularly imprinted polymer nanocomposite material was prepared using multi-walled carbon nanotubes as carriers. The characterization of the obtained nanocomposite material was conducted using Fourier transform infrared spectroscopy, a vibrating sample magnetometer, a thermogravimetric analyzer, a scanning electron microscope, and a transmission electron microscope. The adsorption properties of the nanocomposite material were evaluated through adsorption experiments, including static adsorption, dynamic adsorption, and selective recognition studies. The prepared nanocomposite material, serving as a selective adsorbent, was applied in magnetic solid-phase extraction. Subsequently, the derivatized samples were analyzed for glucose in fish serum using liquid chromatography-tandem mass spectrometry. Under optimal conditions, the detection limit was 0.30 ng/mL, the quantitation limit was 0.99 ng/mL, satisfactory spiked recovery rates were obtained, and the relative standard deviation was less than 1.1%. Using 2-deoxy-D-ribose as the template molecule and a structural analog of glucose allowed us to eliminate the potential template leakage in qualitative and quantitative analyses, effectively avoiding the issues of false positives and potential quantitative errors, compared to traditional methods. A method for detecting glucose levels in fish serum based on molecularly imprinted polymer technology has been successfully developed to determine the stress and health levels of fish.
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Affiliation(s)
- Boxuan Yao
- Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin 150070, China; (B.Y.); (L.G.); (L.H.); (R.L.); (Z.F.); (Z.C.)
- College of Food Science and Engineering, Dalian Ocean University, Dalian 116023, China
| | - Long Gu
- Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin 150070, China; (B.Y.); (L.G.); (L.H.); (R.L.); (Z.F.); (Z.C.)
- College of Food Science and Engineering, Dalian Ocean University, Dalian 116023, China
| | - Li Huang
- Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin 150070, China; (B.Y.); (L.G.); (L.H.); (R.L.); (Z.F.); (Z.C.)
- Supervision, Inspection and Testing Center for Fishery Environment and Aquatic Products (Harbin), Ministry of Agriculture and Rural Affairs, Harbin 150070, China
| | - Ruichun Li
- Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin 150070, China; (B.Y.); (L.G.); (L.H.); (R.L.); (Z.F.); (Z.C.)
- College of Food Science and Engineering, Dalian Ocean University, Dalian 116023, China
| | - Ze Fan
- Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin 150070, China; (B.Y.); (L.G.); (L.H.); (R.L.); (Z.F.); (Z.C.)
| | - Zhongxiang Chen
- Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin 150070, China; (B.Y.); (L.G.); (L.H.); (R.L.); (Z.F.); (Z.C.)
- Supervision, Inspection and Testing Center for Fishery Environment and Aquatic Products (Harbin), Ministry of Agriculture and Rural Affairs, Harbin 150070, China
| | - Dongli Qin
- Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin 150070, China; (B.Y.); (L.G.); (L.H.); (R.L.); (Z.F.); (Z.C.)
- Supervision, Inspection and Testing Center for Fishery Environment and Aquatic Products (Harbin), Ministry of Agriculture and Rural Affairs, Harbin 150070, China
- Key Laboratory of Control of Quality and Safety for Aquatic Products, Ministry of Agriculture and Rural Affairs, Beijing 100141, China
| | - Lei Gao
- Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin 150070, China; (B.Y.); (L.G.); (L.H.); (R.L.); (Z.F.); (Z.C.)
- Supervision, Inspection and Testing Center for Fishery Environment and Aquatic Products (Harbin), Ministry of Agriculture and Rural Affairs, Harbin 150070, China
- Key Laboratory of Control of Quality and Safety for Aquatic Products, Ministry of Agriculture and Rural Affairs, Beijing 100141, China
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Hu Y, Liu J, Pan Q, Shi X, Wu X. Effects of Artificial Sugar Supplementation on the Composition and Nutritional Potency of Honey from Apis cerana. INSECTS 2024; 15:344. [PMID: 38786900 PMCID: PMC11121883 DOI: 10.3390/insects15050344] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Grants] [Track Full Text] [Subscribe] [Scholar Register] [Received: 04/15/2024] [Revised: 05/05/2024] [Accepted: 05/09/2024] [Indexed: 05/25/2024]
Abstract
In the global apiculture industry, reward feeding and supplementary feeding are essential for maintaining bee colonies. Beekeepers provide artificial supplements to their colonies, typically in the form of either a honey-water solution or sugar syrup. Owing to cost considerations associated with beekeeping, most beekeepers opt for sugar syrup. However, the effects of different types of artificial sugar supplements on bee colonies and their subsequent impact on honey composition remain unclear. To address this gap, this study compared the chemical composition, antioxidant capacity, and nutritional potency of three types of honey: honey derived from colonies fed sugar syrup (sugar-based product, SP) or a honey-water solution (honey-sourced honey, HH) and naturally sourced honey (flower-sourced honey, FH), which served as the control. The results revealed that FH outperformed HH and SP in terms of total acidity, sugar content, total protein content, and antioxidant capacity, and HH outperformed SP. Regarding nutritional efficacy, including the lifespan and learning and memory capabilities of worker bees, FH exhibited the best outcomes, with no significant differences observed between HH and SP. This study underscores the importance of sugar source selection in influencing honey quality and emphasizes the potential consequences of substituting honey with sugar syrup in traditional apiculture practices.
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Affiliation(s)
- Yueyang Hu
- Honeybee Research Institute, Jiangxi Agricultural University, Nanchang 330045, China; (Y.H.); (J.L.); (X.S.)
- Jiangxi Province Key Laboratory of Honeybee Biology and Beekeeping, Nanchang 330045, China
- Jiangxi Anyuan Honeybee Science and Technology Backyard, Anyuan 342100, China;
| | - Jianhui Liu
- Honeybee Research Institute, Jiangxi Agricultural University, Nanchang 330045, China; (Y.H.); (J.L.); (X.S.)
- Jiangxi Province Key Laboratory of Honeybee Biology and Beekeeping, Nanchang 330045, China
| | - Qizhong Pan
- Jiangxi Anyuan Honeybee Science and Technology Backyard, Anyuan 342100, China;
- Jiangxi Ganzhou Agricultural College, Ganzhou 341199, China
| | - Xinxin Shi
- Honeybee Research Institute, Jiangxi Agricultural University, Nanchang 330045, China; (Y.H.); (J.L.); (X.S.)
- Jiangxi Province Key Laboratory of Honeybee Biology and Beekeeping, Nanchang 330045, China
| | - Xiaobo Wu
- Honeybee Research Institute, Jiangxi Agricultural University, Nanchang 330045, China; (Y.H.); (J.L.); (X.S.)
- Jiangxi Province Key Laboratory of Honeybee Biology and Beekeeping, Nanchang 330045, China
- Jiangxi Anyuan Honeybee Science and Technology Backyard, Anyuan 342100, China;
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Dave R, Savaliya N, Dobariya A, Patel R, Patel M. A Robust HPLC Approach for Quantitation of Camptothecin in Mesoporous Silica Nanoparticles Matrix and in the Presence of Its Degradation Products. J AOAC Int 2024; 107:387-395. [PMID: 38407421 DOI: 10.1093/jaoacint/qsae013] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [MESH Headings] [Grants] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 11/09/2023] [Revised: 01/23/2024] [Accepted: 02/13/2024] [Indexed: 02/27/2024]
Abstract
BACKGROUND Camptothecin is a potent anticancer drug used for the treatment of various cancers. OBJECTIVE The goal of this research investigation was to develop and validate a new stability-indicating HPLC technique for the quantitative assessment of camptothecin in in-house developed mesoporous silica nanoparticles, a novel nanoformulation matrix for the treatment of cancer. METHOD The Waters Inertsil® HPLC column (C18) was used for the chromatographic separation, with a flow rate of 1 mL/min, a column oven temperature of 40°C, an injection volume of 10 µL, a detection wavelength of 216 nm, and a 10 min runtime overall. An isocratic blend of phosphate buffer (10 mM, pH7.0) and acetonitrile (60:40, v/v) served as the mobile phase. Various stress conditions including acid, alkali, oxidative, photolytic, thermal, and humidity environments were tested for the quantitative estimation of the camptothecin through the proposed method. RESULTS The results demonstrated that the proposed method is specific (peak purity ≥0.999), accurate (99.69-100.64% w/w), precise (RSD, % <2.0), and sensitive (LOD-0.17 µg and LOQ-0.56 µg) in accordance with ICH guideline Q2 (R1). Any unidentified degradation products did not interfere with the drug's estimation. Furthermore, the current method of analysis has eliminated any excipient interference from the matrix effect caused by the numerous excipients of the formulation matrix. CONCLUSIONS To quantify camptothecin for routine assay purposes, this research work offers a novel and straightforward HPLC methodology with optimized chromatographic parameters, contributing to the research and development community while ensuring an appropriate and efficient use of the drug through a variety of nanoformulation for cancer treatment. HIGHLIGHTS The stability-indicating HPLC method was found to be specific and suitable for routine analysis of camptothecin. The absence of any interference from excipients was confirmed by forced degradation studies.
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Affiliation(s)
- Richa Dave
- Charotar University of Science and Technology, Ramanbhai Patel College of Pharmacy, Changa, Gujarat 388421 Anand, India
| | - Neel Savaliya
- Charotar University of Science and Technology, Ramanbhai Patel College of Pharmacy, Changa, Gujarat 388421 Anand, India
| | - Ashish Dobariya
- Charotar University of Science and Technology, Ramanbhai Patel College of Pharmacy, Changa, Gujarat 388421 Anand, India
- Kashiv Bioscience LLC, Analytical Research & Development, Ahmedabad, Gujarat 382210, India
| | - Rashmin Patel
- Charotar University of Science and Technology, Ramanbhai Patel College of Pharmacy, Changa, Gujarat 388421 Anand, India
| | - Mrunali Patel
- Charotar University of Science and Technology, Ramanbhai Patel College of Pharmacy, Changa, Gujarat 388421 Anand, India
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Liu Y, Xing J, Bi X, Shen J, Zhang S, Xu X, Mao L, Lou Y, Wu X, Mu Y. A novel and sensitive method for simultaneous determination of 6 low-calorie bulk sweeteners by HPLC-ELSD. J Chromatogr B Analyt Technol Biomed Life Sci 2024; 1234:124008. [PMID: 38244427 DOI: 10.1016/j.jchromb.2024.124008] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 06/11/2023] [Revised: 12/22/2023] [Accepted: 01/06/2024] [Indexed: 01/22/2024]
Abstract
A novel and sensitive method for the simultaneous analysis of six low-calorie bulk sweeteners (D-allulose, D-tagatose, D-mannitol, mycose, palatinose, and erythritol) without derivatisation was developed using high-performance liquid chromatography-evaporative light scattering detector (HPLC-ELSD). Chromatographic separations were carried out on a Zorbax Original NH2 (5 μm particle size, 250 mm×4.60 mm id, 70 Å) column with flow rate gradient elution with acetonitrile: water (80:20, v/v). Drift tube temperature was set at 50 ℃, the nebuliser carrier gas flow rate was 1.0 mL·min-1, and nitrogen pressure was regulated to 276 kPa with gain:3. The regression equation showed good linearity (R2 = 0.9985-0.9998) for all six low-calorie bulk sweeteners in the tested range (0.060-0.60 mg·mL-1). The limits of detection (LOD) for the six low-calorie bulk sweeteners ranged from 0.02 to 0.06 mg·mL-1. The proposed HPLC-ELSD method was validated for the quantification of the low-calorie bulk sweeteners in 14 types of foods, and the results were satisfactory. In addition, the results showed that the number of sweeteners in each food product varied. The presence of multiple low-calorie bulk sweeteners in certain foods is interesting. This method is successful in monitoring low-calorie bulk sweeteners in food.
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Affiliation(s)
- Yu Liu
- College of Food and Pharmaceutical Sciences, Ningbo University, Ningbo 315211, PR China; Ningbo Academy of Product and Food Quality Inspection (Ningbo Fibre Inspection Institute), Ningbo 315048, PR China
| | - Jiali Xing
- Ningbo Academy of Product and Food Quality Inspection (Ningbo Fibre Inspection Institute), Ningbo 315048, PR China.
| | - Xiaoli Bi
- Ningbo Academy of Product and Food Quality Inspection (Ningbo Fibre Inspection Institute), Ningbo 315048, PR China
| | - Jian Shen
- Ningbo Academy of Product and Food Quality Inspection (Ningbo Fibre Inspection Institute), Ningbo 315048, PR China
| | - Shufen Zhang
- Ningbo Academy of Product and Food Quality Inspection (Ningbo Fibre Inspection Institute), Ningbo 315048, PR China
| | - Xiaorong Xu
- Ningbo Academy of Product and Food Quality Inspection (Ningbo Fibre Inspection Institute), Ningbo 315048, PR China
| | - Lingyan Mao
- Ningbo Academy of Product and Food Quality Inspection (Ningbo Fibre Inspection Institute), Ningbo 315048, PR China
| | - Yongjiang Lou
- College of Food and Pharmaceutical Sciences, Ningbo University, Ningbo 315211, PR China.
| | - Xi Wu
- College of Food and Pharmaceutical Sciences, Ningbo University, Ningbo 315211, PR China
| | - Yinghua Mu
- College of Food and Pharmaceutical Sciences, Ningbo University, Ningbo 315211, PR China
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Huanan G, Shiqin D, Qiaoyan W, Qi Z, Hua Y, Dongxu W. Rapid and sensitive smartphone non-enzymatic colorimetric assay for the detection of glucose in food based on peroxidase-like activity of Fe 3O 4@Au nanoparticles. SPECTROCHIMICA ACTA. PART A, MOLECULAR AND BIOMOLECULAR SPECTROSCOPY 2023; 302:122970. [PMID: 37331256 DOI: 10.1016/j.saa.2023.122970] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.5] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 01/22/2023] [Revised: 05/14/2023] [Accepted: 05/31/2023] [Indexed: 06/20/2023]
Abstract
A low-cost and reliable analytical method based on the combination of a newly designed Fe3O4@Au as peroxidase mimetics, supported on smartphone analysis software package was proposed for the determination of glucose content in food samples. The nanocomposite was prepared by self-assembling technique, and the characterization was carried out using transmission electron microscopy (TEM), Fourier transforms infrared, and X-ray diffractometer. Record the color change of the solution with a smartphone camera and optimize the operation parameters and reaction conditions. A smartphone with a free self-developed app was accustomed live the RGB (red-greenblue) values of color intensity within the Fe3O4@Au system and were processed with Image J software before computationally convert them glucose concentrations. At the optimization experiment, reaction temperature of 60 °C, reaction time of 50 min and the amount of addition of Fe3O4@Au 0.0125 g was the optimal combination of detecting glucose smartphone color detection system. Hereon, the accuracy of the proposed method was evaluated by comparison between smartphone colorimetry and UV-vis spectrophotometer, a linear calibration in the range of 0.25 ∼ 15 mmol/L glucose was obtained with minimum detection limit of 1.83 and 2.25 μmol/L, respectively. The proposed method was applied effectively to the detection of glucose in actual samples. The results were in accordance with the conventional UV-vis spectrophotometer method.
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Affiliation(s)
- Guan Huanan
- School of Gain Science and Technology, Jiangsu University of Science and Technology, Zhenjiang 212000, People's Republic of China; College of Food Engineering, Harbin University of Commerce, Harbin 150076, People's Republic of China.
| | - Du Shiqin
- College of Food Engineering, Harbin University of Commerce, Harbin 150076, People's Republic of China
| | - Wu Qiaoyan
- College of Food Engineering, Harbin University of Commerce, Harbin 150076, People's Republic of China
| | - Zhang Qi
- School of Gain Science and Technology, Jiangsu University of Science and Technology, Zhenjiang 212000, People's Republic of China
| | - Ye Hua
- School of Gain Science and Technology, Jiangsu University of Science and Technology, Zhenjiang 212000, People's Republic of China
| | - Wang Dongxu
- School of Gain Science and Technology, Jiangsu University of Science and Technology, Zhenjiang 212000, People's Republic of China
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Zhang G, Ma C, He Q, Dong H, Cui L, Li L, Li L, Wang Y, Wang X. An efficient Pt@MXene platform for the analysis of small-molecule natural products. iScience 2023; 26:106622. [PMID: 37250310 PMCID: PMC10214401 DOI: 10.1016/j.isci.2023.106622] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 11/12/2022] [Revised: 02/01/2023] [Accepted: 03/31/2023] [Indexed: 05/31/2023] Open
Abstract
Small-molecule (m/z<500) natural products have rich biological activity and significant application value thus need to be effectively detected. Surface-assisted laser desorption/ionization mass spectrometry (SALDI MS) has become a powerful detection tool for small-molecule analysis. However, more efficient substrates need to be developed to improve the efficiency of SALDI MS. Thus, platinum nanoparticle-decorated Ti3C2 MXene (Pt@MXene) was synthesized in this study as an ideal substrate for SALDI MS in positive ion mode and exhibited excellent performance for the high-throughput detection of small molecules. Compared with using MXene, GO, and CHCA matrix, a stronger signal peak intensity and wider molecular coverage was obtained using Pt@MXene in the detection of small-molecule natural products, with a lower background, excellent salt and protein tolerance, good repeatability, and high detection sensitivity. The Pt@MXene substrate was also successfully used to quantify target molecules in medicinal plants. The proposed method has potentially wide application.
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Affiliation(s)
- Guanhua Zhang
- College of Traditional Chinese Medicine, Yunnan University of Chinese Medicine, Kunming, Yunnan 650500, China
- Key Laboratory for Applied Technology of Sophisticated Analytical Instruments of Shandong Province, Shandong Analysis and Test Center, Qilu University of Technology (Shandong Academy of Sciences), Jinan, Shandong 250014, China
| | - Chunxia Ma
- Key Laboratory for Applied Technology of Sophisticated Analytical Instruments of Shandong Province, Shandong Analysis and Test Center, Qilu University of Technology (Shandong Academy of Sciences), Jinan, Shandong 250014, China
| | - Qing He
- Collaborative Innovation Center of Steel Technology, University of Science and Technology Beijing, Beijing 100083, China
| | - Hongjing Dong
- Key Laboratory for Applied Technology of Sophisticated Analytical Instruments of Shandong Province, Shandong Analysis and Test Center, Qilu University of Technology (Shandong Academy of Sciences), Jinan, Shandong 250014, China
| | - Li Cui
- Key Laboratory for Applied Technology of Sophisticated Analytical Instruments of Shandong Province, Shandong Analysis and Test Center, Qilu University of Technology (Shandong Academy of Sciences), Jinan, Shandong 250014, China
| | - Lili Li
- Key Laboratory for Applied Technology of Sophisticated Analytical Instruments of Shandong Province, Shandong Analysis and Test Center, Qilu University of Technology (Shandong Academy of Sciences), Jinan, Shandong 250014, China
| | - Lingyu Li
- Key Laboratory of Food Processing Technology and Quality Control of Shandong Higher Education Institutes, College of Food Science and Engineering, Shandong Agricultural University, Taian, Shandong 271018, China
| | - Yan Wang
- College of Traditional Chinese Medicine, Yunnan University of Chinese Medicine, Kunming, Yunnan 650500, China
| | - Xiao Wang
- College of Traditional Chinese Medicine, Yunnan University of Chinese Medicine, Kunming, Yunnan 650500, China
- Key Laboratory for Applied Technology of Sophisticated Analytical Instruments of Shandong Province, Shandong Analysis and Test Center, Qilu University of Technology (Shandong Academy of Sciences), Jinan, Shandong 250014, China
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Wu CW, Chiang MH, Lee CL. Pd@Au Core-Shell Octahedral, Truncated Octahedral, and Cubic Nanocrystals as Nonenzymatic Glucose Sensors for Drinks. Microchem J 2023. [DOI: 10.1016/j.microc.2023.108697] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 03/30/2023]
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10
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Yan S, Wang W, Zhao W, Tian W, Wang X, Wu L, Xue X. Identification of the maturity of acacia honey by an endogenous oligosaccharide: A preliminary study. Food Chem 2023; 399:134005. [DOI: 10.1016/j.foodchem.2022.134005] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 03/31/2022] [Revised: 07/05/2022] [Accepted: 08/21/2022] [Indexed: 11/16/2022]
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11
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Soyseven M, Sezgin B, Acar E, Arli G. Green, Rapid, Robust Determination of Aspartame in Beverages by High-Performance Liquid Chromatography-Evaporative Light Scattering Detection (HPLC-ELSD): Method Development and Validation. ANAL LETT 2022. [DOI: 10.1080/00032719.2022.2146129] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/18/2022]
Affiliation(s)
- Murat Soyseven
- Department of Medical Services and Techniques, Yunus Emre Vocational School of Health Services, Department of Medical Services and Techniques, Anadolu University, Eskişehir, Turkey
| | - Burcu Sezgin
- Department of Environmental Protection Technologies, Eskişehir Vocational School, Eskişehir Osmangazi University, Eskişehir, Turkey
| | - Ebrar Acar
- Department of Analytical Chemistry, Faculty of Pharmacy, Anadolu University, Eskişehir, Turkey
| | - Göksel Arli
- Department of Medical Services and Techniques, Yunus Emre Vocational School of Health Services, Department of Medical Services and Techniques, Anadolu University, Eskişehir, Turkey
- Department of Analytical Chemistry, Faculty of Pharmacy, Anadolu University, Eskişehir, Turkey
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Shchemelev IS, Khasanov DS, Smirnova MA, Ivanov AV, Ferapontov NB, Tokmachev MG. Determination of reducing carbohydrates in natural honey samples by optical micrometry method. CHIMICA TECHNO ACTA 2022. [DOI: 10.15826/chimtech.2022.9.4.17] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/13/2022] Open
Abstract
The present article deals with the determination of the total amount of glucose and fructose in natural honey samples by the optical micrometry (OM) method. (Poly)vinyl alcohol spherical granules impregnated with a 0.05 mol/l borax solution were chosen as a sensitive element. It was shown that the formation of chelate esters of boron with polymer and carbohydrates is a pH-dependent process, and that the pH range 8.5–10.0 is the most appropriate for quantifying the total amount of reducing carbohydrates because glucose and fructose are undiscriminated. The impregnated polymer is not sensitive to the sucrose presence in the solution due to the absence of cis-diol fragments in it. Subsequently, the OM method was tested in the analysis of natural honey samples. The relative standart deviation in the case of OM method is less than 6%, and the results are similar to those obtained by the iodometric titration method. This makes the OM method suitable for laboratory-scale applications.
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Teixeira GG, Santos PM. Simple and cost-effective approaches for quantification of reducing sugar exploiting digital image analysis. J Food Compost Anal 2022. [DOI: 10.1016/j.jfca.2022.104719] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 10/17/2022]
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Nutritional Comparison of Sacha Inchi (Plukenetia volubilis) Residue with Edible Seeds and Nuts in Taiwan: A Chromatographic and Spectroscopic Study. INTERNATIONAL JOURNAL OF FOOD SCIENCE 2022; 2022:9825551. [PMID: 36245564 PMCID: PMC9553689 DOI: 10.1155/2022/9825551] [Citation(s) in RCA: 2] [Impact Index Per Article: 0.7] [Reference Citation Analysis] [Abstract] [Track Full Text] [Download PDF] [Figures] [Subscribe] [Scholar Register] [Received: 04/25/2022] [Revised: 07/29/2022] [Accepted: 08/24/2022] [Indexed: 11/18/2022]
Abstract
Sacha inchi is a source of quality commercial oil in Taiwan. Oil extraction results in sacha inchi residue have not been utilized and not much investigated. Different edible seeds and nuts have different levels of nutrients. This study aims (a) to determine the oil, moisture, ash, protein, carbohydrate, type of fatty acid, resveratrol, and type of sugar in edible seeds and nuts, including sacha inchi residue, and (b) to determine the model to predict the five macronutrients using NIR spectroscopy. The samples used were candlenut, peanut, sesame, sunflower, sacha inchi residue, and black bean. Determination was conducted using NIR spectroscopy, NMR spectroscopy, LC-MS/MS, and HPLC-ELSD. NIR spectroscopy prediction results show that candlenut is rich in oil, and sacha inchi residue is rich in minerals, protein, and moisture. The correct prediction model for oil and moisture is principal component regression, while partial least squares are for ash, protein, and carbohydrates. NMR spectroscopy results showed that all samples were rich in polyunsaturated fatty acids. Sacha inchi residue is rich in omega 3. LC-MS/MS results showed that all samples contained resveratrol, and its highest level was found in sesame. HPLC-ELSD results showed eight types of sugars in the samples. High sucrose was found in sacha inchi residue, sunflower, sesame, and candlenut. The results are expected to provide information on nutrient levels in seeds and nuts to consumers and people who deal with nutrition. Also, results are expected to increase the economic value of sacha inchi residue as a source of diversification of food products in Taiwan.
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Separation of Monosaccharide Anomers on Photo-Click Cysteine-Based Stationary Phase: The α/β Interconversion Process Studied by Dynamic Hydrophilic Liquid Chromatography. SEPARATIONS 2022. [DOI: 10.3390/separations9080203] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/16/2022] Open
Abstract
In High-Performance Liquid Chromatography (HPLC), the separation of reducing sugars can typically show three possible typologies of chromatographic profiles (i.e., single peak, two resolved peaks and two peaks interconnected by a plateau) due to the rate at which the relevant α/β anomers interconversion (anomerization) can take place in relation to their elution-time. By analyzing these chromatographic profiles, thermodynamic and kinetic properties of anomerization phenomenon can be extrapolated. In this work we studied the anomerization of some monosaccharides by using a recently developed photo-click cysteine-based stationary phase through dynamic hydrophilic interaction liquid chromatography (D-HILIC) conditions. In the 5–25 °C temperature range, the ΔG#α→β and ΔG#β→α barriers were found to achieve values within the interval 21.1/22.2 kcal/mol for glucose, with differences between α→β and β→α reactions of about 0.4 kcal/mol. For xylose, in the same temperature range, the ΔG#α→β and ΔG#β→α barriers are between 20.7 to 21.5 kcal/mol, with differences between α→β and β→α reactions of about 0.2 kcal/mol. The experimental data are in agreement with those reported in literature, confirming the this new stationary phase using HILIC conditions is a robust platform to measure kinetic and thermodynamic properties of the isomerization reaction.
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Koulis GA, Tsagkaris AS, Katsianou PA, Gialouris PLP, Martakos I, Stergiou F, Fiore A, Panagopoulou EI, Karabournioti S, Baessmann C, van der Borg N, Dasenaki ME, Proestos C, Thomaidis NS. Thorough Investigation of the Phenolic Profile of Reputable Greek Honey Varieties: Varietal Discrimination and Floral Markers Identification Using Liquid Chromatography–High-Resolution Mass Spectrometry. Molecules 2022; 27:molecules27144444. [PMID: 35889316 PMCID: PMC9323402 DOI: 10.3390/molecules27144444] [Citation(s) in RCA: 9] [Impact Index Per Article: 3.0] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 06/08/2022] [Revised: 07/04/2022] [Accepted: 07/05/2022] [Indexed: 01/27/2023] Open
Abstract
Honey is a highly consumed commodity due to its potential health benefits upon certain consumption, resulting in a high market price. This fact indicates the need to protect honey from fraudulent acts by delivering comprehensive analytical methodologies. In this study, targeted, suspect and non-targeted metabolomic workflows were applied to identify botanical origin markers of Greek honey. Blossom honey samples (n = 62) and the unifloral fir (n = 10), oak (n = 24), pine (n = 39) and thyme (n = 34) honeys were analyzed using an ultra-high-performance liquid chromatography hybrid quadrupole time-of-flight mass spectrometry (UHPLC-q-TOF-MS) system. Several potential authenticity markers were revealed from the application of different metabolomic workflows. In detail, based on quantitative targeted analysis, three blossom honey markers were found, namely, galangin, pinocembrin and chrysin, while gallic acid concentration was found to be significantly higher in oak honey. Using suspect screening workflow, 12 additional bioactive compounds were identified and semi-quantified, achieving comprehensive metabolomic honey characterization. Lastly, by combining non-targeted screening with advanced chemometrics, it was possible to discriminate thyme from blossom honey and develop binary discriminatory models with high predictive power. In conclusion, a holistic approach to assessing the botanical origin of Greek honey is presented, highlighting the complementarity of the three applied metabolomic approaches.
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Affiliation(s)
- Georgios A. Koulis
- Analytical Chemistry Laboratory, Chemistry Department, National and Kapodistrian University of Athens, Panepistimiopolis Zographou, 15771 Athens, Greece; (G.A.K.); (P.A.K.); (P.-L.P.G.); (I.M.); (F.S.); (E.I.P.)
- Food Chemistry Laboratory, Department of Chemistry, National and Kapodistrian University of Athens, Panepistimiopolis Zographou, 15771 Athens, Greece;
| | - Aristeidis S. Tsagkaris
- Department of Food Analysis and Nutrition, Faculty of Food and Biochemical Technology, University of Chemistry and Technology Prague, Technická 5, 16628 Prague, Czech Republic;
| | - Panagiota A. Katsianou
- Analytical Chemistry Laboratory, Chemistry Department, National and Kapodistrian University of Athens, Panepistimiopolis Zographou, 15771 Athens, Greece; (G.A.K.); (P.A.K.); (P.-L.P.G.); (I.M.); (F.S.); (E.I.P.)
| | - Panagiotis-Loukas P. Gialouris
- Analytical Chemistry Laboratory, Chemistry Department, National and Kapodistrian University of Athens, Panepistimiopolis Zographou, 15771 Athens, Greece; (G.A.K.); (P.A.K.); (P.-L.P.G.); (I.M.); (F.S.); (E.I.P.)
- Food Chemistry Laboratory, Department of Chemistry, National and Kapodistrian University of Athens, Panepistimiopolis Zographou, 15771 Athens, Greece;
| | - Ioannis Martakos
- Analytical Chemistry Laboratory, Chemistry Department, National and Kapodistrian University of Athens, Panepistimiopolis Zographou, 15771 Athens, Greece; (G.A.K.); (P.A.K.); (P.-L.P.G.); (I.M.); (F.S.); (E.I.P.)
- Food Chemistry Laboratory, Department of Chemistry, National and Kapodistrian University of Athens, Panepistimiopolis Zographou, 15771 Athens, Greece;
| | - Fotis Stergiou
- Analytical Chemistry Laboratory, Chemistry Department, National and Kapodistrian University of Athens, Panepistimiopolis Zographou, 15771 Athens, Greece; (G.A.K.); (P.A.K.); (P.-L.P.G.); (I.M.); (F.S.); (E.I.P.)
- Division of Engineering and Food Science, School of Applied Science, Abertay University, Bell Street, Dundee DD1 1HG, UK;
| | - Alberto Fiore
- Division of Engineering and Food Science, School of Applied Science, Abertay University, Bell Street, Dundee DD1 1HG, UK;
| | - Eleni I. Panagopoulou
- Analytical Chemistry Laboratory, Chemistry Department, National and Kapodistrian University of Athens, Panepistimiopolis Zographou, 15771 Athens, Greece; (G.A.K.); (P.A.K.); (P.-L.P.G.); (I.M.); (F.S.); (E.I.P.)
| | | | - Carsten Baessmann
- Bruker Daltonik GmbH, Fahrenheitstraße 4, 28359 Bremen, Germany; (C.B.); (N.v.d.B.)
| | - Noud van der Borg
- Bruker Daltonik GmbH, Fahrenheitstraße 4, 28359 Bremen, Germany; (C.B.); (N.v.d.B.)
| | - Marilena E. Dasenaki
- Food Chemistry Laboratory, Department of Chemistry, National and Kapodistrian University of Athens, Panepistimiopolis Zographou, 15771 Athens, Greece;
- Correspondence: (M.E.D.); (N.S.T.); Tel.: +30-210-727-4326 (M.E.D.); +30-210-727-4430 (N.S.T.)
| | - Charalampos Proestos
- Food Chemistry Laboratory, Department of Chemistry, National and Kapodistrian University of Athens, Panepistimiopolis Zographou, 15771 Athens, Greece;
| | - Nikolaos S. Thomaidis
- Analytical Chemistry Laboratory, Chemistry Department, National and Kapodistrian University of Athens, Panepistimiopolis Zographou, 15771 Athens, Greece; (G.A.K.); (P.A.K.); (P.-L.P.G.); (I.M.); (F.S.); (E.I.P.)
- Correspondence: (M.E.D.); (N.S.T.); Tel.: +30-210-727-4326 (M.E.D.); +30-210-727-4430 (N.S.T.)
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Kurzyna-Szklarek M, Cybulska J, Zdunek A. Analysis of the chemical composition of natural carbohydrates - An overview of methods. Food Chem 2022; 394:133466. [PMID: 35716502 DOI: 10.1016/j.foodchem.2022.133466] [Citation(s) in RCA: 30] [Impact Index Per Article: 10.0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 02/23/2022] [Revised: 05/13/2022] [Accepted: 06/09/2022] [Indexed: 11/19/2022]
Abstract
Natural carbohydrates are gaining importance over a wide spectrum of human activity due to their versatile functionalities. The properties of carbohydrates are currently used in many branches of industry and new possibilities of their utilization, like in medicine or materials science, are demonstrated systematically. The attractive properties of carbohydrates result from their chemical structure and ability to form macromolecules and derivatives. Each application of carbohydrate requires a knowledge of their chemical composition, which due to the number and differentiation of monosaccharides and their spatial forms is often challenging. This review presents an overview on sample preparation and the methods used for the determination of the fine chemical structure of natural carbohydrates. Most popular and reliable colorimetric, chromatographic and spectroscopic methods are presented with an emphasis on their pros and cons.
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Affiliation(s)
| | - Justyna Cybulska
- Institute of Agrophysics, Polish Academy of Sciences, Doświadczalna 4, 20-290 Lublin, Poland.
| | - Artur Zdunek
- Institute of Agrophysics, Polish Academy of Sciences, Doświadczalna 4, 20-290 Lublin, Poland
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