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Tekin A, Tornacı S, Boyacı D, Li S, Calligaris S, Maalej H, Toksoy Öner E. Hydrogels of levan polysaccharide: A systematic review. Int J Biol Macromol 2025; 315:144430. [PMID: 40409642 DOI: 10.1016/j.ijbiomac.2025.144430] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 03/28/2025] [Revised: 05/07/2025] [Accepted: 05/18/2025] [Indexed: 05/25/2025]
Abstract
Levan is a fructose-based homopolysaccharide renowned for its unique properties, including exceptional adhesive strength, self-assembly capability, low viscosity, and bioactivities such as prebiotic, anti-cancer, anti-inflammatory, and anti-diabetic effects. These characteristics have created increasing interest in levan-based biomaterials over the past decade, positioning levan as a highly under-explored biopolymer for a wide range of applications, from medicine to cosmetics. As a result, levan-based hydrogels have emerged as promising biomaterials in drug delivery, tissue engineering, and cosmetic formulations, owing to their extracellular matrix-mimicking structure, tunable mechanical properties, and controlled cargo release capabilities. This review is the first to comprehensively examine the advancements in levan-based hydrogel research, systematically analyzing their biomedical applications and comparing them with other biopolymer-based hydrogels. Key questions regarding levan's potential as an alternative to established hydrogel systems are explored, highlighting areas requiring further research. By assessing trends and findings in the literature, this review provides an overview of the advantages, limitations, and prospects of levan hydrogels. Our analysis establishes a foundation for the continued development of levan-derived biomaterials, fostering broader adoption in biomedical and industrial applications.
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Affiliation(s)
- Aybüke Tekin
- IBSB, Marmara University, Department of Bioengineering, Istanbul, Turkey
| | - Selay Tornacı
- IBSB, Marmara University, Department of Bioengineering, Istanbul, Turkey
| | - Defne Boyacı
- Uskudar American Academy, 34664, Uskudar, Istanbul, Turkey
| | - Suming Li
- Institut Européen des Membranes, UMR CNRS 5635, Université de Montpellier, France
| | - Sonia Calligaris
- Department of Agricultural, Food, Environmental and Animal Sciences, University of Udine, Udine 33100, Italy
| | - Hana Maalej
- Laboratory of Biodiversity and Valorization of Arid Areas Bioresources (BVBAA), LR16ES36, Faculty of Sciences of Gabes, University of Gabes, Gabes 6072, Tunisia
| | - Ebru Toksoy Öner
- IBSB, Marmara University, Department of Bioengineering, Istanbul, Turkey.
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Jiang Y, Zhou T, Zhang S, Leng J, Li L, Zhao W. β-Glucan-based superabsorbent hydrogel ameliorates obesity-associated metabolic disorders via delaying gastric emptying, improving intestinal barrier function, and modulating gut microbiota. Int J Biol Macromol 2025; 304:140846. [PMID: 39933677 DOI: 10.1016/j.ijbiomac.2025.140846] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 11/13/2024] [Revised: 01/25/2025] [Accepted: 02/07/2025] [Indexed: 02/13/2025]
Abstract
The global obesity epidemic and its associated metabolic syndrome highlight the urgent need for new weight-loss therapies that provide high efficacy and patient compliance. Herein, we propose a novel, noninvasive approach using an orally administered β-glucan-based superabsorbent hydrogel (βC-MA hydrogel) to improve obesity-associated metabolic disorders. Results demonstrated that βC-MA hydrogel functioned as a dynamic exoskeleton within the gastrointestinal tract, slowing gastric emptying and reducing the digestion and absorption of ingested food. Furthermore, βC-MA hydrogel alleviated hepatic lipid accumulation and prevented hepatic steatosis and fibrosis by regulating the expression levels of key genes involved in lipid metabolism, including Cd36, SREBP 1c, FAS, ACC1, Cpt1a, and HSL, thereby limiting the progression of nonalcoholic fatty liver disease. In addition, βC-MA hydrogel reduced intestinal inflammation by lowering tumor necrosis factor-α and interleukin-6 levels while enhancing gut barrier function through increased expression of claudin-1, ZO-1, and MUC2. Finally, βC-MA hydrogel, enriched with obesity-negative probiotics such as Akkermansia, norank_f__Muribaculaceae, and Faecalibaculum, promoted the production of short-chain fatty acids. Consequently, βC-MA hydrogel significantly reduced body weight and fat accumulation and improved blood glucose and lipid levels, with efficacy comparable to semaglutide therapy and superior to β-glucan and sodium carboxymethylcellulose interventions. Overall, these findings suggest that βC-MA hydrogel could serve as a promising next-generation ingestible medical device for alleviating diet-induced obesity and related metabolic disorders by modulating food digestion and absorption, improving intestinal inflammation and barrier function, and regulating gut microbiota composition.
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Affiliation(s)
- Yiming Jiang
- State Key Laboratory of Food Science and Resources, School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, PR China
| | - Tingyi Zhou
- State Key Laboratory of Food Science and Resources, School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, PR China
| | - Shiqi Zhang
- State Key Laboratory of Food Science and Resources, School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, PR China
| | - Juncai Leng
- State Key Laboratory of Food Science and Resources, School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, PR China
| | - Li Li
- State Key Laboratory of Food Science and Resources, School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, PR China
| | - Wei Zhao
- State Key Laboratory of Food Science and Resources, School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, PR China.
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Veerapandian B, Krishnan S, Sivaraman S, Immanuel A, Shanmugam SR, Toksoy Öner E, Venkatachalam P, Ulaganathan V. Bacillus spp. as microbial factories for levan and fructooligosaccharide production - Recent trends. Int J Biol Macromol 2025; 300:140252. [PMID: 39863230 DOI: 10.1016/j.ijbiomac.2025.140252] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 09/26/2024] [Revised: 12/25/2024] [Accepted: 01/21/2025] [Indexed: 01/27/2025]
Abstract
Levan is a fructan-type homopolysaccharide that has gained increasing attention due to its unique properties and promising applications. It is a fructose-based polymer produced through microbial fermentation by diverse microorganisms, including bacteria, yeasts and archaea. The ongoing research on levan mainly focuses on optimizing production processes, elucidating its biological functions, and uncover novel applications. Studies on novel microbial producer strains and fermentation conditions have led to advancements in production processes. Remarkably, the production of levan using the native producer Bacillus spp. has shown promising trends in yield and molecular weight distribution of levan and levan-type fructooligosaccharides. Ongoing studies on levan highlight the importance of understanding the complexities of levan production for commercial scalability. The present article explores the potential of Bacillus spp. as native levan producers, presenting insights into synthesis mechanisms, factors influencing the production of levan, and genetic modifications aimed at enhancing production efficiency and altering properties.
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Affiliation(s)
- Bhuvaneshwari Veerapandian
- Biomass conversion and Bioproducts Laboratory, Center for Bioenergy, School of Chemical & Biotechnology, SASTRA Deemed University, Thirumalaisamudram, Tamil Nadu, India
| | - Srividhya Krishnan
- Biomass conversion and Bioproducts Laboratory, Center for Bioenergy, School of Chemical & Biotechnology, SASTRA Deemed University, Thirumalaisamudram, Tamil Nadu, India
| | - Subramaniyasharma Sivaraman
- Biomass conversion and Bioproducts Laboratory, Center for Bioenergy, School of Chemical & Biotechnology, SASTRA Deemed University, Thirumalaisamudram, Tamil Nadu, India
| | - Aruldoss Immanuel
- School of Chemical & Biotechnology, SASTRA Deemed University, Thirumalaisamudram, Tamil Nadu, India
| | | | - Ebru Toksoy Öner
- IBSB, Department of Bioengineering, Marmara University, Istanbul, Turkey
| | - Ponnusami Venkatachalam
- Biomass conversion and Bioproducts Laboratory, Center for Bioenergy, School of Chemical & Biotechnology, SASTRA Deemed University, Thirumalaisamudram, Tamil Nadu, India.
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Ahmad W, Nasir A, Prakash S, Hayat A, Rehman MU, Khaliq S, Akhtar K, Anwar MA, Munawar N. In Vitro and In Vivo Interventions Reveal the Health Benefits of Levan-Type Exopolysaccharide Produced by a Fish Gut Isolate Lactobacillus reuteri FW2. Life (Basel) 2025; 15:89. [PMID: 39860029 PMCID: PMC11767011 DOI: 10.3390/life15010089] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 07/01/2024] [Revised: 01/07/2025] [Accepted: 01/09/2025] [Indexed: 01/27/2025] Open
Abstract
Microorganisms synthesize diverse types of exopolysaccharides (EPSs). EPSs with varying structural and physical properties can demonstrate unique health benefits, which allow for their tailored applications as functional foods such as prebiotics. Levan, a fructose-based EPS, is gaining considerable attention as an effective prebiotic to support the growth of beneficial gut bacteria. Consequently, this enhances digestive health, boosts the immune system, and reduces the risk of chronic diseases. Unfortunately, limited studies are available on levan-type EPSs to demonstrate their role as prebiotics. Therefore, in this study, we conducted in vitro and in vivo experiments, concerning intestinal cell integrity and metabolic syndrome, to assess the therapeutic potential of levan derived from Lactobacillus reuteri FW2. The in vitro experimental results revealed that levan improved the survival of impaired HT-29 epithelial cells of the intestine and also exerted antioxidant effects. In the in vivo experiments, mice fed with levan-supplemented feed exhibited low body weight gain, blood glucose, and serum cholesterol levels compared to the control group. These findings highlight the biotherapeutic potential of L. reuteri FW2-derived levan for improving metabolic syndrome and its associated aspects. It also signifies the need for a further detailed investigation based on clinical trials to include levan in dietary supplements for improved health and well-being.
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Affiliation(s)
- Waqar Ahmad
- Industrial Biotechnology Division, National Institute for Biotechnology and Genetic Engineering College, Pakistan Institute of Engineering and Applied Sciences (NIBGE-C, PIEAS), Faisalabad 38000, Pakistan; (W.A.); (A.N.); (S.K.); (K.A.)
- Biomedical Technology and Cell Therapy Research Laboratory, Department of Biomedical Engineering, Faculty of Medicine, McGill University, 3775 University Street, Montreal, QC H3A 2B4, Canada;
- Department of Microbiology, Abbottabad University of Science and Technology, Havelian, Abbottabad 22500, Pakistan; (A.H.); (M.u.R.)
| | - Anam Nasir
- Industrial Biotechnology Division, National Institute for Biotechnology and Genetic Engineering College, Pakistan Institute of Engineering and Applied Sciences (NIBGE-C, PIEAS), Faisalabad 38000, Pakistan; (W.A.); (A.N.); (S.K.); (K.A.)
- Department of Chemistry, College of Sciences, United Arab Emirates University, Al-Ain 15551, United Arab Emirates
| | - Satya Prakash
- Biomedical Technology and Cell Therapy Research Laboratory, Department of Biomedical Engineering, Faculty of Medicine, McGill University, 3775 University Street, Montreal, QC H3A 2B4, Canada;
| | - Azam Hayat
- Department of Microbiology, Abbottabad University of Science and Technology, Havelian, Abbottabad 22500, Pakistan; (A.H.); (M.u.R.)
| | - Mujaddad ur Rehman
- Department of Microbiology, Abbottabad University of Science and Technology, Havelian, Abbottabad 22500, Pakistan; (A.H.); (M.u.R.)
| | - Shazia Khaliq
- Industrial Biotechnology Division, National Institute for Biotechnology and Genetic Engineering College, Pakistan Institute of Engineering and Applied Sciences (NIBGE-C, PIEAS), Faisalabad 38000, Pakistan; (W.A.); (A.N.); (S.K.); (K.A.)
| | - Kalsoom Akhtar
- Industrial Biotechnology Division, National Institute for Biotechnology and Genetic Engineering College, Pakistan Institute of Engineering and Applied Sciences (NIBGE-C, PIEAS), Faisalabad 38000, Pakistan; (W.A.); (A.N.); (S.K.); (K.A.)
| | - Munir Ahmad Anwar
- Industrial Biotechnology Division, National Institute for Biotechnology and Genetic Engineering College, Pakistan Institute of Engineering and Applied Sciences (NIBGE-C, PIEAS), Faisalabad 38000, Pakistan; (W.A.); (A.N.); (S.K.); (K.A.)
| | - Nayla Munawar
- Department of Chemistry, College of Sciences, United Arab Emirates University, Al-Ain 15551, United Arab Emirates
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Jawad I, Bin Tawseen H, Irfan M, Ahmad W, Hassan M, Sattar F, Awan FR, Khaliq S, Akhtar N, Akhtar K, Anwar MA, Munawar N. Dietary Supplementation of Microbial Dextran and Inulin Exerts Hypocholesterolemic Effects and Modulates Gut Microbiota in BALB/c Mice Models. Int J Mol Sci 2023; 24:ijms24065314. [PMID: 36982388 PMCID: PMC10049499 DOI: 10.3390/ijms24065314] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 01/15/2023] [Revised: 03/02/2023] [Accepted: 03/05/2023] [Indexed: 03/12/2023] Open
Abstract
Microbial exopolysaccharides (EPSs), having great structural diversity, have gained tremendous interest for their prebiotic effects. In the present study, mice models were used to investigate if microbial dextran and inulin-type EPSs could also play role in the modulation of microbiomics and metabolomics by improving certain biochemical parameters, such as blood cholesterol and glucose levels and weight gain. Feeding the mice for 21 days on EPS-supplemented feed resulted in only 7.6 ± 0.8% weight gain in the inulin-fed mice group, while the dextran-fed group also showed a low weight gain trend as compared to the control group. Blood glucose levels of the dextran- and inulin-fed groups did not change significantly in comparison with the control where it increased by 22 ± 5%. Moreover, the dextran and inulin exerted pronounced hypocholesterolemic effects by reducing the serum cholesterol levels by 23% and 13%, respectively. The control group was found to be mainly populated with Enterococcus faecalis, Staphylococcus gallinarum, Mammaliicoccus lentus and Klebsiella aerogenes. The colonization of E. faecalis was inhibited by 59–65% while the intestinal release of Escherichia fergusonii was increased by 85–95% in the EPS-supplemented groups, respectively, along with the complete inhibition of growth of other enteropathogens. Additionally, higher populations of lactic acid bacteria were detected in the intestine of EPS-fed mice as compared to controls.
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Affiliation(s)
- Iqra Jawad
- Industrial Biotechnology Division, National Institute for Biotechnology and Genetic Engineering College, Pakistan Institute of Engineering and Applied Sciences (NIBGE-C, PIEAS), Faisalabad 38000, Pakistan
| | - Husam Bin Tawseen
- Industrial Biotechnology Division, National Institute for Biotechnology and Genetic Engineering College, Pakistan Institute of Engineering and Applied Sciences (NIBGE-C, PIEAS), Faisalabad 38000, Pakistan
| | - Muhammad Irfan
- Industrial Biotechnology Division, National Institute for Biotechnology and Genetic Engineering College, Pakistan Institute of Engineering and Applied Sciences (NIBGE-C, PIEAS), Faisalabad 38000, Pakistan
| | - Waqar Ahmad
- Industrial Biotechnology Division, National Institute for Biotechnology and Genetic Engineering College, Pakistan Institute of Engineering and Applied Sciences (NIBGE-C, PIEAS), Faisalabad 38000, Pakistan
- Department of Microbiology, Abbottabad University of Science and Technology, Havelian, Abbottabad 22020, Pakistan
| | - Mujtaba Hassan
- Health Biotechnology Division, National Institute for Biotechnology and Genetic Engineering College, Pakistan Institute of Engineering and Applied Sciences (NIBGE-C, PIEAS), Faisalabad 38000, Pakistan
| | - Fazal Sattar
- Industrial Biotechnology Division, National Institute for Biotechnology and Genetic Engineering College, Pakistan Institute of Engineering and Applied Sciences (NIBGE-C, PIEAS), Faisalabad 38000, Pakistan
| | - Fazli Rabbi Awan
- Health Biotechnology Division, National Institute for Biotechnology and Genetic Engineering College, Pakistan Institute of Engineering and Applied Sciences (NIBGE-C, PIEAS), Faisalabad 38000, Pakistan
| | - Shazia Khaliq
- Industrial Biotechnology Division, National Institute for Biotechnology and Genetic Engineering College, Pakistan Institute of Engineering and Applied Sciences (NIBGE-C, PIEAS), Faisalabad 38000, Pakistan
| | - Nasrin Akhtar
- Industrial Biotechnology Division, National Institute for Biotechnology and Genetic Engineering College, Pakistan Institute of Engineering and Applied Sciences (NIBGE-C, PIEAS), Faisalabad 38000, Pakistan
| | - Kalsoom Akhtar
- Industrial Biotechnology Division, National Institute for Biotechnology and Genetic Engineering College, Pakistan Institute of Engineering and Applied Sciences (NIBGE-C, PIEAS), Faisalabad 38000, Pakistan
| | - Munir Ahmad Anwar
- Industrial Biotechnology Division, National Institute for Biotechnology and Genetic Engineering College, Pakistan Institute of Engineering and Applied Sciences (NIBGE-C, PIEAS), Faisalabad 38000, Pakistan
- Correspondence: or (M.A.A.); (N.M.); Tel.: +92-41-920-1316 (M.A.A.); +971-3-713-6168 (N.M.); Fax: +92-41-920-1322 (M.A.A.)
| | - Nayla Munawar
- Department of Chemistry, College of Science, United Arab Emirates University (UAEU), Al Ain 15551, United Arab Emirates
- Correspondence: or (M.A.A.); (N.M.); Tel.: +92-41-920-1316 (M.A.A.); +971-3-713-6168 (N.M.); Fax: +92-41-920-1322 (M.A.A.)
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Chen S, Tong Q, Guo X, Cong H, Zhao Z, Liang W, Li J, Zhu P, Yang H. Complete secretion of recombinant Bacillus subtilis levansucrase in Pichia pastoris for production of high molecular weight levan. Int J Biol Macromol 2022; 214:203-211. [PMID: 35714864 DOI: 10.1016/j.ijbiomac.2022.06.092] [Citation(s) in RCA: 8] [Impact Index Per Article: 2.7] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 03/30/2022] [Revised: 06/03/2022] [Accepted: 06/12/2022] [Indexed: 11/17/2022]
Abstract
Three signal peptides from α-mating factor (α-MF), inulinase (INU) and native levansucrase (LS) were compared for secretion efficiency of Bacillus subtilis levansucrase SacB-T305A in Pichia pastoris GS115. The first complete secretion of bacterial levansucrase in yeasts under methanol induction was achieved while using α-MF signal. The secreted recombinant Lev(α-MF) proved to be glycosylated by combination of NanoLC-MS/MS and Endo H digestion. Interestingly, glycosylation not only improved significantly the polymerase thermostability, but also reversed the products profiles to favor synthesis of high molecular weight (HMW) levan which accounted for approximately 73 % to total levan-type polysaccharides. It indicated for the first time that the glycosylation of recombinant B. subtilis levansucrase affected significantly the products molecular weight distribution. It also provided a promising enzymatic way to effectively product HMW levan from sucrose resources.
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Affiliation(s)
- Shuochang Chen
- College of Life Science and Technology, Guangxi University, 100 Daxue Road, Nanning, Guangxi 530004, China; State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangxi University, 100 Daxue Road, Nanning, Guangxi 530004, China; Guangxi Research Center for Microbial and Enzyme Engineering Technology, 100 Daxue Road, Nanning, Guangxi 530004, China
| | - Qiuping Tong
- College of Life Science and Technology, Guangxi University, 100 Daxue Road, Nanning, Guangxi 530004, China
| | - Xiaolei Guo
- College of Life Science and Technology, Guangxi University, 100 Daxue Road, Nanning, Guangxi 530004, China; State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangxi University, 100 Daxue Road, Nanning, Guangxi 530004, China; Guangxi Research Center for Microbial and Enzyme Engineering Technology, 100 Daxue Road, Nanning, Guangxi 530004, China
| | - Hao Cong
- College of Life Science and Technology, Guangxi University, 100 Daxue Road, Nanning, Guangxi 530004, China
| | - Zi Zhao
- College of Life Science and Technology, Guangxi University, 100 Daxue Road, Nanning, Guangxi 530004, China; State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangxi University, 100 Daxue Road, Nanning, Guangxi 530004, China; Guangxi Research Center for Microbial and Enzyme Engineering Technology, 100 Daxue Road, Nanning, Guangxi 530004, China
| | - Wenfeng Liang
- College of Life Science and Technology, Guangxi University, 100 Daxue Road, Nanning, Guangxi 530004, China; State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangxi University, 100 Daxue Road, Nanning, Guangxi 530004, China; Guangxi Research Center for Microbial and Enzyme Engineering Technology, 100 Daxue Road, Nanning, Guangxi 530004, China
| | - Jiemin Li
- Agro-food Science and Technology Research Institute, Guangxi Academy of Agricultural Sciences, 174 East Daxue Road, Nanning, Guangxi 530007, China
| | - Ping Zhu
- College of Life Science and Technology, Guangxi University, 100 Daxue Road, Nanning, Guangxi 530004, China
| | - Hui Yang
- College of Life Science and Technology, Guangxi University, 100 Daxue Road, Nanning, Guangxi 530004, China; State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangxi University, 100 Daxue Road, Nanning, Guangxi 530004, China; Guangxi Research Center for Microbial and Enzyme Engineering Technology, 100 Daxue Road, Nanning, Guangxi 530004, China.
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Jatuponwiphat T, Namrak T, Nitisinprasert S, Nakphaichit M, Vongsangnak W. Integrative growth physiology and transcriptome profiling of probiotic Limosilactobacillus reuteri KUB-AC5. PeerJ 2021; 9:e12226. [PMID: 34707932 PMCID: PMC8500091 DOI: 10.7717/peerj.12226] [Citation(s) in RCA: 2] [Impact Index Per Article: 0.5] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 06/03/2021] [Accepted: 09/08/2021] [Indexed: 12/26/2022] Open
Abstract
Limosilactobacillus reuteri KUB-AC5 has been widely used as probiotic in chicken for Salmonella reduction. However, a preferable carbon source and growth phase is poorly characterized underlying metabolic responses on growth and inhibition effects of L. reuteri KUB-AC5. This study therefore aimed to investigate transcriptome profiling of L. reuteri KUB-AC5 revealing global metabolic responses when alteration of carbon sources and growth phases. Interestingly, L. reuteri KUB-AC5 grown under sucrose culture showed to be the best for fast growth and inhibition effects against Salmonella Enteritidis S003 growth. Towards the transcriptome profiling and reporter proteins/metabolites analysis, the results showed that amino acid transport via ABC systems as well as sucrose metabolism and transport are key metabolic responses at Logarithmic (L)-phase of L. reuteri KUB-AC5 growth. Considering the Stationary (S)-phase, we found the potential reporter proteins/metabolites involved in carbohydrate metabolism e.g., levansucrase and levan. Promisingly, levansucrase and levan were revealed to be candidates in relation to inhibition effects of L. reuteri KUB-AC5. Throughout this study, L. reuteri KUB-AC5 had a metabolic control in acclimatization to sucrose and energy pools through transcriptional co-regulation, which supported the cell growth and inhibition potentials. This study offers a perspective in optimizing fermentation condition through either genetic or physiological approaches for enhancing probiotic L. reuteri KUB-AC5 properties.
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Affiliation(s)
- Theeraphol Jatuponwiphat
- Interdisciplinary Graduate Program in Bioscience, Faculty of Science, Kasetsart University, Bangkok, Thailand
| | - Thanawat Namrak
- Department of Biotechnology, Faculty of Agro-Industry, Kasetsart University, Bangkok, Thailand
| | - Sunee Nitisinprasert
- Department of Biotechnology, Faculty of Agro-Industry, Kasetsart University, Bangkok, Thailand
| | - Massalin Nakphaichit
- Department of Biotechnology, Faculty of Agro-Industry, Kasetsart University, Bangkok, Thailand
| | - Wanwipa Vongsangnak
- Department of Zoology, Faculty of Science, Kasetsart University, Bangkok, Thailand.,Omics Center for Agriculture, Bioresources, Food, and Health, Kasetsart University (OmiKU), Bangkok, Thailand
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Li J, Wang Y, Zhang J, Bai Y, Gao J. Fructans from Codonopsis pilosula Roots. Chem Nat Compd 2021. [DOI: 10.1007/s10600-021-03433-8] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/29/2022]
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9
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Optimization and extraction of edible microbial polysaccharide from fresh coconut inflorescence sap: An alternative substrate. Lebensm Wiss Technol 2021. [DOI: 10.1016/j.lwt.2020.110619] [Citation(s) in RCA: 14] [Impact Index Per Article: 3.5] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 01/08/2023]
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A Fructan Sucrase Secreted Extracellular and Purified in One-Step by Gram-Positive Enhancer Matrix Particles. Processes (Basel) 2021. [DOI: 10.3390/pr9010095] [Citation(s) in RCA: 3] [Impact Index Per Article: 0.8] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 01/11/2023] Open
Abstract
Fructan sucrase is a kind of biological enzyme that catalyzes the synthesis of fructan, and fructan is a polysaccharide product with important industrial application value. In this study, the Fructan sucrase gene of Bacillus subtilis was cloned to plasmid PET-28A-ACMA-Z, and three clones were obtained after the transformation of Escherichia coli BL21, namely BS-FF, BSO, and BS. The clones BS-FF and BSO secreted the recombinant enzymes outside the cells, while the clone BS expressed them inside the cells. The induction experiment results showed that the optimum IPTG concentration in the medium was 0.5 mM and 1.0 mM for clones BS-FF and BSO, respectively, while the incubation conditions were at 28 °C for 8 h. The recombinant fructan sucrase was purified one step using a material called GEM particles. The results indicated that 95.25% of fructan sucrase expressed by the clone BS-FF could be secreted into the extracellular area, and even 98.78% by the clone BSO. With the above purification system, the receiving rate of the recombinant enzyme for clones BS-FF and BSO was 97.70% and 84.99%, respectively. As for the bioactivity of recombinant fructan sucrase, the optimum temperature and pH were 50 °C and 5.6, respectively. The Km and Vmax of it were 33.96 g/L and 0.63 g/(L·min), respectively. The engineered strains with the high extracellular secretion of fructan sucrase were constructed, and a one-step method for the purification of the recombinant enzyme was established. The results might provide a novel selection for the enzymatic production of fructan on a large scale.
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Molecular weight dependent structure of the exopolysaccharide levan. Int J Biol Macromol 2020; 161:398-405. [DOI: 10.1016/j.ijbiomac.2020.06.019] [Citation(s) in RCA: 10] [Impact Index Per Article: 2.0] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 03/20/2020] [Revised: 05/26/2020] [Accepted: 06/02/2020] [Indexed: 11/22/2022]
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12
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Li J, Wang Y, Zhang X, Cao L, Ji J, Zheng Q, Gao J. Isolation and structural identification of a novel fructan from Radix Codonopsis. J Carbohydr Chem 2020. [DOI: 10.1080/07328303.2020.1772278] [Citation(s) in RCA: 3] [Impact Index Per Article: 0.6] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 10/24/2022]
Affiliation(s)
- Jiankuan Li
- School of Pharmaceutical Science, Shanxi Medical University, Taiyuan, China
| | - Yan Wang
- School of Pharmaceutical Science, Shanxi Medical University, Taiyuan, China
| | - Xia Zhang
- School of Pharmaceutical Science, Shanxi Medical University, Taiyuan, China
| | - Lingya Cao
- School of Pharmaceutical Science, Shanxi Medical University, Taiyuan, China
- School of Basic Medical Science, Shanxi Medical University, Taiyuan, China
| | - Jiaojiao Ji
- School of Pharmaceutical Science, Shanxi Medical University, Taiyuan, China
| | - Qinghong Zheng
- School of Pharmaceutical Science, Shanxi Medical University, Taiyuan, China
| | - Jianping Gao
- School of Pharmaceutical Science, Shanxi Medical University, Taiyuan, China
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Catalytic biosynthesis of levan and short-chain fructooligosaccharides from sucrose-containing feedstocks by employing the levansucrase from Leuconostoc mesenteroides MTCC10508. Int J Biol Macromol 2019; 127:486-495. [DOI: 10.1016/j.ijbiomac.2019.01.070] [Citation(s) in RCA: 23] [Impact Index Per Article: 3.8] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 10/15/2018] [Revised: 01/14/2019] [Accepted: 01/16/2019] [Indexed: 01/23/2023]
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Zhang Y, Xu L, Ding M, Su G, Zhao Y. Anti-obesity effect of garlic oil on obese rats via Shenque point administration. JOURNAL OF ETHNOPHARMACOLOGY 2019; 231:486-493. [PMID: 30472401 DOI: 10.1016/j.jep.2018.11.030] [Citation(s) in RCA: 14] [Impact Index Per Article: 2.3] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 04/16/2018] [Revised: 11/15/2018] [Accepted: 11/20/2018] [Indexed: 06/09/2023]
Abstract
ETHNOPHARMACOLOGICAL RELEVANCE Shenque is an acupoint located in the umbilicus and connected with the meridians. Thus, acupoint herbs applied at Shenque plays a pivotal role in the Chinese traditional medicine due to its sensitivity, permeability, and absorption. Many studies reported the use of Shenque point as a successful therapeutic approach. However, the effect of garlic oil (GO) applied at Shenque point to combat obesity is unmet. Consequently, we investigated the potential benefit of GO applied at Shenque point against obesity. AIM OF THE STUDY To investigate GO effects on obese rats applied at Shenque acupoint and orally administered, and to identify the chemical constituents of GO. MATERIALS AND METHODS Rats were randomly divided into 2 groups: naive and model group. The model group rats were fed with a high fat diet for 7 weeks to induce obesity, and then they were randomly divided into 5 groups: model, GO Shenque point treated groups (25, 50 and 100 mg/kg/day) and oral group (50 mg/kg/day). Biochemical indexes in the serum, weight of adipose tissue and liver histopathology were evaluated after 6 weeks of GO treatment using a Hitachi 7080 analyzer (Hitachi, Japan). Moreover, GO chemical components were detected by gas chromatography-mass spectrometer (GC-MS). RESULTS Compared with the naive rats, model rats exhibited higher body and liver weight, increased fat deposition, higher triglyceride concentration and alveolar development. In contrast, GO Shenque point treated groups showed a substantial decrease in body weight (P = 0.358, 0.028, 0.031, respectively), fat mass, cholesterol (P = 0.004, 0.041, 0.001, respectively), triglyceride (P = 0.001, 0.001, 0.001, respectively), and low density lipoprotein concentrations (P = 0.001, 0.000, 0.001, respectively). The effect was more remarkable than the GO orally administered. In addition, twelve GO organosulfur compounds were identified by GC-MS and diallyl trisulfide (DATS) was detected as the main compound, with a 32.08% concentration. CONCLUSIONS These findings demonstrated that GO had a significant anti-obesity effect on obese rats by reducing the body weight and protecting the liver from damage, and the effect of Shenque point treatment was better than oral administration, suggesting that GO was an effective weight-loss drug and Shenque point administration might be considered as a new anti-obesity approach.
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Affiliation(s)
- Yumeng Zhang
- Shenyang Pharmaceutical University, Shenyang 110016, China
| | - Lei Xu
- Yanbian University, Yanji 133000, China
| | - Meng Ding
- Shenyang Pharmaceutical University, Shenyang 110016, China
| | - Guangyue Su
- Shenyang Pharmaceutical University, Shenyang 110016, China; Key Laboratory of Structure-based Drug Design and Discovery of Ministry of Education, Shenyang Pharmaceutical University, Shenyang 110016, China.
| | - Yuqing Zhao
- Shenyang Pharmaceutical University, Shenyang 110016, China; Key Laboratory of Structure-based Drug Design and Discovery of Ministry of Education, Shenyang Pharmaceutical University, Shenyang 110016, China.
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Joaquim EO, Hayashi AH, Torres LMB, Figueiredo-Ribeiro RCL, Shiomi N, de Sousa FS, Lago JHG, Carvalho MAM. Chemical Structure and Localization of Levan, the Predominant Fructan Type in Underground Systems of Gomphrena marginata (Amaranthaceae). FRONTIERS IN PLANT SCIENCE 2018; 9:1745. [PMID: 30564253 PMCID: PMC6288709 DOI: 10.3389/fpls.2018.01745] [Citation(s) in RCA: 10] [Impact Index Per Article: 1.4] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Figures] [Subscribe] [Scholar Register] [Received: 08/17/2018] [Accepted: 11/09/2018] [Indexed: 06/09/2023]
Abstract
Gomphrena marginata Seub. (Amaranthaceae) is an endemic species from Brazilian campos rupestres with a fructan accumulating underground reserve system. Analyses of high performance anion exchange chromatography (HPAEC-PAD) revealed the presence of the soluble carbohydrates glucose, fructose, sucrose, 1-kestose, 6-kestose, nystose and fructans with degree of polymerization (DP) up to approximately 40 fructose units. Data of 1H and 13C Nuclear Magnetic Resonance (NMR) spectroscopy, including Heteronuclear Single-Quantum Correlation (HSQC) and Heteronuclear Multiple-Bonds Correlation (HMBC) showed the presence of β (2,6) linkages, characteristic of the linear molecule of levan-type fructan(2,6). These results confirmed previous studies suggesting that the reserve carbohydrate in the underground system of this species was levan-type fructans, similar to that of G. macrocephala. Structural analyses of the thickened underground system using light microscopy revealed a mixed origin system consisting mainly of a gemmiferous tuberous root with the upper region formed by short branched stems, both presenting vascular cylinders with unusual growth patterns. Fructan spherocrystals were visualized under polarized light and scanning electron microscopy (SEM) mostly in the cortex and vascular cylinder in both thickened stem and root. In addition to data reported in the literature concerning the occurrence of fructans in the Amaranthaceae, the results presented here suggest that fructans are a trait in this family while the levan-type fructan prevail in Gomphrena species.
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Affiliation(s)
- Emanuela O. Joaquim
- Programa de Pós-graduação em Biodiversidade Vegetal e Meio Ambiente, São Paulo, Brazil
- Núcleo de Pesquisa em Fisiologia e Bioquímica, Instituto de Botânica, São Paulo, Brazil
| | - Adriana H. Hayashi
- Núcleo de Pesquisa em Anatomia, Instituto de Botânica, São Paulo, Brazil
| | - Luce M. B. Torres
- Núcleo de Pesquisa em Fisiologia e Bioquímica, Instituto de Botânica, São Paulo, Brazil
| | | | - Norio Shiomi
- Department of Food and Nutrition Sciences, Graduate School of Dairy Science Research, Rakuno Gakuen University, Ebetsu, Japan
| | - Fernanda S. de Sousa
- Instituto de Ciências Ambientais, Químicas e Farmacêuticas, Universidade Federal de São Paulo, São Paulo, Brazil
| | - João H. G. Lago
- Centro de Ciências Naturais e Humanas, Universidade Federal do ABC, Santo André, Brazil
| | - Maria A. M. Carvalho
- Núcleo de Pesquisa em Fisiologia e Bioquímica, Instituto de Botânica, São Paulo, Brazil
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Influence of acid depolymerization parameters on levan molar mass distribution and its utilization by bacteria. Carbohydr Polym 2018; 206:371-379. [PMID: 30553334 DOI: 10.1016/j.carbpol.2018.11.029] [Citation(s) in RCA: 4] [Impact Index Per Article: 0.6] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 09/01/2018] [Revised: 10/17/2018] [Accepted: 11/09/2018] [Indexed: 12/18/2022]
Abstract
Levan is a fructan composed of β -(2, 6) linkages in its main chain. Its health properties, especially its prebiotic potential can be partially modified by changing its molar mass distribution. Given that native levan is rarely fermented by probiotic bacteria, especially lactic acid bacteria (LAB), levanoligosaccharides (LOS) were produced by mild acid hydrolysis. The response surface methodology (RSM) was applied to determine the optimum parameters for depolymerization. Gel permeation chromatography (GPC) was used to characterize the LOS produced and to show the differences between inulin and levan. The prebiotic potential of four fractions of LOS with different molar mass distributions was investigated. MRS (Mann Rogosa Sharpe) medium supplemented with the LOS were inoculated with bacterial strains and growth was monitored by measuring the turbidity of the cultures. The utilization of oligofructans was also confirmed by RP-UHPLC-UV-ESI-MS (liquid chromatography coupled with mass spectrometry) measurements of LOS derivatized with 1-phenyl-3-methyl-5-pyrazolone (PMP). It was observed that the degree of polymerization of LOS has an influence on the growth of the tested bacteria.
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17
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Biosynthesis of levan from sucrose using a thermostable levansucrase from Lactobacillus reuteri LTH5448. Int J Biol Macromol 2018; 113:29-37. [DOI: 10.1016/j.ijbiomac.2018.01.187] [Citation(s) in RCA: 41] [Impact Index Per Article: 5.9] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 12/04/2017] [Revised: 01/21/2018] [Accepted: 01/29/2018] [Indexed: 11/18/2022]
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18
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Lassoued I, Mezghani M, Jridi M, Rahmouni F, Jamoussi K, Rebai T, El Feki A, Nasri M, Barkia A. Protective effects of thornback ray muscle protein hydrolysate against dyslipidemia, oxidative stress and reduced fertility induced by high cholesterol diet in adult male rats. RSC Adv 2018; 8:22303-22312. [PMID: 35539733 PMCID: PMC9081093 DOI: 10.1039/c8ra00657a] [Citation(s) in RCA: 5] [Impact Index Per Article: 0.7] [Reference Citation Analysis] [Abstract] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 01/22/2018] [Accepted: 06/03/2018] [Indexed: 12/31/2022] Open
Abstract
Enzymatic thornback ray (Raja clavata) muscle hydrolysates have been shown to have antioxidant and antihypertensive activities in vitro. The Neutrase hydrolysate exhibited the highest activities, so it was investigated along with the undigested muscle to test their hypolipidemic, antioxidative and fertility effects in rats fed with a high-cholesterol diet (HCD). Animals were allocated into four groups of 5 rats each: a normal diet group (control), a HCD group, and two groups of HCD with a daily dose of undigested muscle (Und) or the hydrolysate (MH) at 0.7 g kg−1 of body weight. All animals received their respective treatments daily for 1 month. After the treatment period, serum lipid profiles, the activities of alanine aminotransferase and aspartate aminotransferase, the level of malonaldehyde, the activities of antioxidant enzymes (catalase and glutathione peroxidase) in the liver and sperm fertility parameters (in the epididymis and testis) were determined. Compared with those fed a standard diet, HCD induced dyslipidemia and oxidative stress, and decreased numerous reproductive parameters (mobility, count and viability). Interestingly, supplementing the HCD with thornback ray proteins attenuated all these anomalies, especially in the case where they were hydrolysed. These observations suggested that these proteins might contain bioactive peptides that possess hypocholesterolemic and antioxidant activities that ameliorate sperm damage. Thornback ray muscle hydrolysate was investigated, as well as the undigested muscle, to test their hypolipidemic, antioxidative and fertility effects in rats fed with a high-cholesterol diet.![]()
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Affiliation(s)
- Imen Lassoued
- Laboratory of Enzyme Engineering and Microbiology
- University of Sfax
- National Engineering School of Sfax
- B.P. 1173-3038 Sfax
- Tunisia
| | - Mayassa Mezghani
- Laboratory of Enzyme Engineering and Microbiology
- University of Sfax
- National Engineering School of Sfax
- B.P. 1173-3038 Sfax
- Tunisia
| | - Mourad Jridi
- Laboratory of Enzyme Engineering and Microbiology
- University of Sfax
- National Engineering School of Sfax
- B.P. 1173-3038 Sfax
- Tunisia
| | - Fatma Rahmouni
- Histology-Embryology Laboratory
- Sfax Faculty of Medicine
- Sfax
- Tunisia
| | - Kamel Jamoussi
- Clinical Chemistry Laboratory
- Hedi Chaker University Hospital
- Sfax
- Tunisia
| | - Tarek Rebai
- Histology-Embryology Laboratory
- Sfax Faculty of Medicine
- Sfax
- Tunisia
| | - Abdelfattah El Feki
- Animal Ecophysiology Laboratory
- Sciences Faculty of Sfax
- University of Sfax
- Tunisia
| | - Moncef Nasri
- Laboratory of Enzyme Engineering and Microbiology
- University of Sfax
- National Engineering School of Sfax
- B.P. 1173-3038 Sfax
- Tunisia
| | - Ahmed Barkia
- Laboratory of Enzyme Engineering and Microbiology
- University of Sfax
- National Engineering School of Sfax
- B.P. 1173-3038 Sfax
- Tunisia
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Shen TT, Wu SX. Effects of Tea Seed Oil on Hyperlipidemic Rats Induced by High-fat Diet. FOOD SCIENCE AND TECHNOLOGY RESEARCH 2017. [DOI: 10.3136/fstr.23.101] [Citation(s) in RCA: 3] [Impact Index Per Article: 0.4] [Reference Citation Analysis] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 11/03/2022]
Affiliation(s)
- Tian-Tian Shen
- School of chemistry and bioengineering, Changsha University of Science & Technology
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Samout N, Ettaya A, Bouzenna H, Ncib S, Elfeki A, Hfaiedh N. Beneficial effects of Plantago albicans on high-fat diet-induced obesity in rats. Biomed Pharmacother 2016; 84:1768-1775. [PMID: 27876214 DOI: 10.1016/j.biopha.2016.10.105] [Citation(s) in RCA: 9] [Impact Index Per Article: 1.0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 08/18/2016] [Revised: 10/28/2016] [Accepted: 10/30/2016] [Indexed: 10/20/2022] Open
Abstract
Obesity is a one of the main global public health problems associated with chronic diseases such as coronary heart disease, diabetes and cancer. As a solution to obesity, we suggest Plantago albicans, which is a medicinal plant with several biological effects. This study assesses the possible anti-obesity protective properties of Plantago albicans in high fat diet-fed rats. 28 male Wistar rats were divided into 4 groups; a group which received normal diet (C), the second group was fed HDF diet (HDF), the third group was given normal diet supplemented with Plantago albicans (P.AL), and the fourth group received HDF supplemented with Plantago albicans (HDF+P.AL) (30mg/kg/day) for 7 weeks. Our results showed an increase in body weight of HDF rats by ∼16% as compared to the control group with an increase in the levels of total cholesterol (TC) as well as LDL-cholesterol, triglycerides (TG) in serum. Also, the concentration of TBARS increased in the liver and heart of HDF-fed rats as compared to the control group. The oral gavage of Plantago albicans extract to obese rats induced a reduction in their body weight, lipid accumulation in liver and heart tissue, compared to the high-fat diet control rats. The obtained results proved that the antioxidant potency of Plantago albicans extracts was correlated with their phenolic and flavonoid contents. The antioxidant capacity of the extract was evaluated by DPPH test (as EC50=250±2.12μg/mL) and FRAP tests (as EC50=27.77±0.14μg/mL). These results confirm the phytochemical and antioxidant impact of Plantago albicans extracts. Plantago albicans content was determined using validated HPLC methodology.
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Affiliation(s)
- Noura Samout
- Laboratory of Environmental Physiopathology, Valorization of Bioactive Molecules and Mathematical Modeling, Faculty of Sciences of Sfax, Road Soukra km 3.5- PB n° 1171-3000, Sfax, Tunisia; Laboratory Animal Eco physiology, Faculty of sciences, Sidi Ahmed Zarrouk, 2112, Gafsa, Tunisia.
| | - Amani Ettaya
- Laboratory of Environmental Physiopathology, Valorization of Bioactive Molecules and Mathematical Modeling, Faculty of Sciences of Sfax, Road Soukra km 3.5- PB n° 1171-3000, Sfax, Tunisia; Laboratory Animal Eco physiology, Faculty of sciences, Sidi Ahmed Zarrouk, 2112, Gafsa, Tunisia
| | - Hafsia Bouzenna
- Laboratory of Environmental Physiopathology, Valorization of Bioactive Molecules and Mathematical Modeling, Faculty of Sciences of Sfax, Road Soukra km 3.5- PB n° 1171-3000, Sfax, Tunisia; Laboratory Animal Eco physiology, Faculty of sciences, Sidi Ahmed Zarrouk, 2112, Gafsa, Tunisia
| | - Sana Ncib
- Unity of Commun Services, Faculty of sciences, Sidi Ahmed Zarrouk, 2112, Gafsa, Tunisia
| | - Abdelfattah Elfeki
- Laboratory of Environmental Physiopathology, Valorization of Bioactive Molecules and Mathematical Modeling, Faculty of Sciences of Sfax, Road Soukra km 3.5- PB n° 1171-3000, Sfax, Tunisia
| | - Najla Hfaiedh
- Laboratory of Environmental Physiopathology, Valorization of Bioactive Molecules and Mathematical Modeling, Faculty of Sciences of Sfax, Road Soukra km 3.5- PB n° 1171-3000, Sfax, Tunisia; Laboratory Animal Eco physiology, Faculty of sciences, Sidi Ahmed Zarrouk, 2112, Gafsa, Tunisia
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Öner ET, Hernández L, Combie J. Review of Levan polysaccharide: From a century of past experiences to future prospects. Biotechnol Adv 2016; 34:827-844. [DOI: 10.1016/j.biotechadv.2016.05.002] [Citation(s) in RCA: 246] [Impact Index Per Article: 27.3] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 01/07/2016] [Revised: 05/01/2016] [Accepted: 05/04/2016] [Indexed: 01/24/2023]
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23
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Review on production, characterization and applications of microbial levan. Carbohydr Polym 2015; 120:102-14. [DOI: 10.1016/j.carbpol.2014.12.003] [Citation(s) in RCA: 160] [Impact Index Per Article: 16.0] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 09/22/2014] [Revised: 10/27/2014] [Accepted: 12/05/2014] [Indexed: 11/24/2022]
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Abstract
This chapter describes the various compounds that can act as prebiotic fibers: their structure, occurrence, production, and physiological effects (health effects) will be presented. The basis for the description is the latest definitions for dietary fibers and for prebiotics. Using as much as possible data from human studies, both the fiber and the prebiotic properties will be described of a variety of compounds. Based on the presented data the latest developments in the area of prebiotics, fibers and gut and immune health will be discussed in more detail as they show best what the potential impact of prebiotics on health of the human host might be.
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Ben Abdallah Kolsi R, Ben Gara A, Chaaben R, El Feki A, Patti FP, El Feki L, Belghith K. Anti-obesity and lipid lowering effects of Cymodocea nodosa sulphated polysaccharide on high cholesterol-fed-rats. Arch Physiol Biochem 2015; 121:210-7. [PMID: 26599414 DOI: 10.3109/13813455.2015.1105266] [Citation(s) in RCA: 26] [Impact Index Per Article: 2.6] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Indexed: 01/16/2023]
Abstract
This study aims to evaluate for the first time the effects of Cymodocea nodosa sulphated polysaccharide (CNSP) on lipase activity in vitro and in vivo to high fat diet (HFD)-rats on body weight, lipid profile and liver-kidney functions. The administration of CNSP decreases the body weight and inhibits lipase activity of obese rats in serum and intestine as compared with untreated HDF-rats. This decrease in lipase activity leads to lipid regulation shown by the decrease of total cholesterol (T-Ch), triglycerides (TG) and low density lipoprotein cholesterol (LDL-C) and an increase in high density lipoprotein cholesterol (HDL-C) levels in HFD-rats. Additionally, CNSP administration to HFD-rats induces anti-oxidant activity observed by the increase of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX) activities and the decrease in Thiobarbituric acid reactive substances (TBARS) levels and protects liver-kidney functions proven by a decrease in the levels of toxicity parameters in blood.
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Affiliation(s)
- Rihab Ben Abdallah Kolsi
- a Laboratory of Plant Biotechnology , Faculty of Sciences of Sfax , Sfax , Tunisia
- d Stazione Zoologica 'A. Dohrn', Functional and Evolutionary Ecology Laboratory , Punta S. Pietro, Ischia (Naples) , Italy
| | - Amel Ben Gara
- a Laboratory of Plant Biotechnology , Faculty of Sciences of Sfax , Sfax , Tunisia
| | - Rim Chaaben
- b Biochemistry Laboratory , CHU Hedi Chaker , Sfax , Tunisia
| | - Abdelfattah El Feki
- c Laboratory of Animal Ecophysiology , Faculty of Sciences of Sfax , Tunisia , and
| | - Francesco Paolo Patti
- d Stazione Zoologica 'A. Dohrn', Functional and Evolutionary Ecology Laboratory , Punta S. Pietro, Ischia (Naples) , Italy
| | - Lotfi El Feki
- a Laboratory of Plant Biotechnology , Faculty of Sciences of Sfax , Sfax , Tunisia
| | - Karima Belghith
- a Laboratory of Plant Biotechnology , Faculty of Sciences of Sfax , Sfax , Tunisia
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Kazak Sarilmiser H, Toksoy Oner E. Investigation of anti-cancer activity of linear and aldehyde-activated levan from Halomonas smyrnensis AAD6T. Biochem Eng J 2014. [DOI: 10.1016/j.bej.2014.06.020] [Citation(s) in RCA: 65] [Impact Index Per Article: 5.9] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 12/31/2022]
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Polysaccharide Production by Submerged Fermentation. POLYSACCHARIDES 2014. [DOI: 10.1007/978-3-319-03751-6_39-1] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 10/24/2022] Open
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Dahech I, Fakhfakh J, Damak M, Belghith H, Mejdoub H, Belghith KS. Structural determination and NMR characterization of a bacterial exopolysaccharide. Int J Biol Macromol 2013; 59:417-22. [DOI: 10.1016/j.ijbiomac.2013.04.036] [Citation(s) in RCA: 19] [Impact Index Per Article: 1.6] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 02/28/2013] [Revised: 03/30/2013] [Accepted: 04/12/2013] [Indexed: 10/26/2022]
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Wang LX, Liu K, Gao DW, Hao JK. Protective effects of two Lactobacillus plantarum strains in hyperlipidemic mice. World J Gastroenterol 2013; 19:3150-3156. [PMID: 23716997 PMCID: PMC3662957 DOI: 10.3748/wjg.v19.i20.3150] [Citation(s) in RCA: 46] [Impact Index Per Article: 3.8] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Download PDF] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Received: 12/07/2012] [Revised: 01/17/2013] [Accepted: 03/01/2013] [Indexed: 02/06/2023] Open
Abstract
AIM: To investigate the effects of Lactobacillus plantarum (L. plantarum) CAI6 and L. plantarum SC4 on hyperlipidemic mice.
METHODS: Male Kunming mice were fed a high-cholesterol diet for 28 d to construct hyperlipidemic models. Hyperlipidemic mice and normal mice were assigned to 3 groups which were separately treated with L. plantarum CAI6, L. plantarum SC4, and physiological saline through oral gavage for 28 d. Total cholesterol (TC), triglycerides (TG), high-density lipoprotein cholesterol (HDL-C), and low-density lipoprotein cholesterol (LDL-C) levels were measured by commercially available enzyme kits. FACS Calibur flow cytometry was used to examine hepatic and renal nuclear factor-erythroid 2-related factor 2 (Nrf2) expression. The morphology of livers was checked by hematoxylin and eosin staining and optical microscope observation.
RESULTS: Compared with normal mice, hyperlipidemic mice possessed significantly higher TC (3.50 ± 0.43 vs 2.89 ± 0.36, P < 0.01), TG (1.76 ± 0.07 vs 1.10 ± 0.16, P < 0.01), and LDL-C (1.72 ± 0.20 vs 0.82 ± 0.10, P< 0.01) levels, resulting in an increase of atherogenic index (AI) (2.34 ± 1.60 vs 0.93 ± 0.55, P < 0.05) and LDL-C/HDL-C ratio (1.43 ± 0.12 vs 0.51 ± 0.16, P < 0.05). After treatment with L. plantarum CAI6/L. plantarum SC4, TG (1.43 ± 0.27/1.54 ± 0.10 vs 1.76 ± 0.07, P < 0.01/P < 0.05) and LDL-C (1.42 ± 0.07/1.47 ± 0.12 vs 1.72 ± 0.20, P < 0.01/P < 0.01) in hyperlipidemic mice significantly decreased. In addition, TC, HDL-C, AI, and LDL-C/HDL-C ratio were all positively changed. Meanwhile, the treatment markedly alleviated hepatic steatosis and significantly stimulated Nrf2 expression (73.79 ± 0.80/72.96 ± 1.22 vs 54.94 ± 1.84, P < 0.01/P < 0.01) in hepatocytes of hyperlipidemic mice.
CONCLUSION: L. plantarum CAI6 and L. plantarum SC4 may protect against cardiovascular disease by lipid metabolism regulation and Nrf2-induced antioxidative defense in hyperlipidemic mice.
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Dahech I, Harrabi B, Hamden K, Feki A, Mejdoub H, Belghith H, Belghith KS. Antioxidant effect of nondigestible levan and its impact on cardiovascular disease and atherosclerosis. Int J Biol Macromol 2013; 58:281-6. [PMID: 23624165 DOI: 10.1016/j.ijbiomac.2013.04.058] [Citation(s) in RCA: 40] [Impact Index Per Article: 3.3] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 03/13/2013] [Revised: 04/14/2013] [Accepted: 04/17/2013] [Indexed: 11/16/2022]
Abstract
Levan polysaccharide, a type of fructan, has been shown to favorably affect diabetes type 2 and hypercholesterolemia. Recent reports have indicated that excessive oxidative stress contributes to the development of atherosclerosis linked metabolic syndrome. The objective of this current study was to investigate the possible protection against oxidative stress linked atherosclerosis. A group of twenty four male rats was divided into four subgroups; a normal diet group (Control), normal rats received levan (L), a high-cholesterol diet group (Chol) and a high-cholesterol diet with 5% (w/w) levan group. After the treatment period, the plasma antioxidant enzymes and lipid profiles were determined. Our results show that treatment with levan positively changed plasma antioxidant enzyme activities by increasing superoxide dismutase (SOD) and catalase (CAT) by 40% and 28%, respectively, in heart. Similarly, the treatment of Chol fed groups with levan positively changed lipid profiles by decreasing total cholesterol, triglycerides and LDL-cholesterol by 50%, 38.33% and 64%, respectively. Thus may have potential antioxidant effects and could protect against oxidative stress linked atherosclerosis.
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Affiliation(s)
- Imen Dahech
- Biochemistry Laboratory, Faculty of Sciences of Sfax, Sfax, Tunisia.
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Yu CH, Dai XY, Chen Q, Zang JN, Deng LL, Liu YH, Ying HZ. Hypolipidemic and antioxidant activities of polysaccharides from Rosae Laevigatae Fructus in rats. Carbohydr Polym 2013; 94:56-62. [DOI: 10.1016/j.carbpol.2013.01.006] [Citation(s) in RCA: 36] [Impact Index Per Article: 3.0] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 09/25/2012] [Revised: 01/02/2013] [Accepted: 01/04/2013] [Indexed: 12/18/2022]
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