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1
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Shams F, Jamshidian M, Shaygani H, Maleki S, Soltani M, Shamloo A. A study on the cellular adhesion properties of a hybrid scaffold for vascular tissue engineering through molecular dynamics simulation. Sci Rep 2025; 15:16433. [PMID: 40355635 PMCID: PMC12069603 DOI: 10.1038/s41598-025-01545-7] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 10/11/2024] [Accepted: 05/07/2025] [Indexed: 05/14/2025] Open
Abstract
Utilizing biocompatible hybrid scaffolds that promote cell adhesion and proliferation is critically significant in the field of tissue engineering. In order to achieve this goal, the composition of polymers in the sample should be adjusted accordingly In this research, molecular dynamics simulations are utilized to investigate how the composition of blends influences the protein adsorption properties of hybrid scaffolds. Scaffolds considered here consist of Bombyx mori silk fibroin (B. mori SF) and thermoplastic polyurethane (TPU) intended for application in vascular grafts. Three different compositions are investigated in this study: One sample with 70% TPU by volume (SF:TPU-3/7), the second sample with 50% TPU (SF:TPU-1/1) and the last sample with 30% TPU (SF:TPU-7/3). The interaction between the polymeric scaffold surfaces and fibronectin and laminin, two major proteins found in vascular tissues, is studied using molecular dynamics simulations. The biocompatibility of each sample is examined based on calculated adhesion energy and final protein conformation. Furthermore, MTT cell viability, cell adhesion, and live/dead assays are performed to validate the simulation results. Third-passage human umbilical vein cell (HUVEC) is utilized in this study. The simulations revealed that B. mori SF (SF) content in the blend needs to be balanced with TPU to enhance the protein adsorption strength. The experimental results exhibited a correlation with the simulations and were verified with cell adhesion and staining assays. The SF:TPU-1/1 had the highest cell viability followed by SF:TPU-7/3 and SF:TPU-3/7 with [Formula: see text], [Formula: see text], and [Formula: see text], respectively, demonstrating the accuracy of the simulations and the possibility of predicting the biocompatibility of biomaterials through simulations.
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Affiliation(s)
- Faeze Shams
- Nano-Bioengineering laboratory, Department of Mechanical Engineering, Sharif University of Technology, Tehran, 11365-11155, Iran
- Stem Cell and Regenerative Medicine Center, Sharif University of Technology, Tehran, 11365-11155, Iran
| | - Mostafa Jamshidian
- Nano-Bioengineering laboratory, Department of Mechanical Engineering, Sharif University of Technology, Tehran, 11365-11155, Iran
| | - Hossein Shaygani
- Nano-Bioengineering laboratory, Department of Mechanical Engineering, Sharif University of Technology, Tehran, 11365-11155, Iran
- Stem Cell and Regenerative Medicine Center, Sharif University of Technology, Tehran, 11365-11155, Iran
| | - Sasan Maleki
- Nano-Bioengineering laboratory, Department of Mechanical Engineering, Sharif University of Technology, Tehran, 11365-11155, Iran
| | - Mohamadreza Soltani
- Nano-Bioengineering laboratory, Department of Mechanical Engineering, Sharif University of Technology, Tehran, 11365-11155, Iran
- Stem Cell and Regenerative Medicine Center, Sharif University of Technology, Tehran, 11365-11155, Iran
| | - Amir Shamloo
- Nano-Bioengineering laboratory, Department of Mechanical Engineering, Sharif University of Technology, Tehran, 11365-11155, Iran.
- Stem Cell and Regenerative Medicine Center, Sharif University of Technology, Tehran, 11365-11155, Iran.
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2
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Karim S, Wells LA. Azobenzene-Grafted Acrylate Coatings to Modulate Lens Epithelial Cells. ACS Biomater Sci Eng 2025; 11:2127-2145. [PMID: 40163533 DOI: 10.1021/acsbiomaterials.4c02214] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 04/02/2025]
Abstract
Polymeric intraocular lenses (IOLs) are prosthetics used to replace cataracts to restore vision. However, in 20% or more of cases, lens epithelial cells (LECs) remaining after surgery migrate along the IOL and posterior capsule, causing new vision anomalies, termed posterior capsule opacification (PCO). The surface of the polymeric IOL is identified as a leading factor for the development of their failure, and we hypothesize that specialized coatings could mitigate or prevent these failures. Azobenzene was grafted to coatings made of poly(methacrylic acid-co-isodecyl acrylate) (MAAcoIDA) and poly(methyl methacrylate-co-isodecyl acrylate) (MMcoIDA) to produce a library of acrylic coatings. The azobenzene on the surface of these coatings could reversibly photoisomerize with 365 nm light and complex with β-cyclodextrin (β-CD). Human LEC cell line, B3-LECs, grown on these coatings had modulated protein and gene expression, with lower α-smooth muscle actin protein expression and inflammatory interleukin 6 gene expression in cells incubated on all of the variations of MMcoIDA compared to MAAcoIDA. Azobenzene modifications with and without UV and β-CD treatment also modulated cell behavior where cells on azobenzene-modified MAAcoIDA had decreased live/dead ratios after UV treatments, a potential method to reduce LEC viability. The cells on β-CD-treated azobenzene-modified MAAcoIDA had differences in cell adhesion after UV treatments, illustrating that UV light can be applied to modulate cell behavior in conjunction with β-CD. The different coatings present methods to modulate LEC adhesion, death, and behavior, temporarily when dependent on UV treatments.
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Affiliation(s)
- Sumaiya Karim
- Department of Chemical Engineering, Queen's University, Kingston, ON K7L 3N6, Canada
| | - Laura A Wells
- Department of Chemical Engineering, Queen's University, Kingston, ON K7L 3N6, Canada
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3
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Chen X, Xu J, Qin F, Yang Z, Li X, Yu M, Li M, Wang Y, Xin W. An immunoregulation PLGA/Chitosan aligned nanofibers with polydopamine coupling basic fibroblast growth factor and ROS scavenging for peripheral nerve regeneration. Mater Today Bio 2025; 31:101543. [PMID: 40026623 PMCID: PMC11869013 DOI: 10.1016/j.mtbio.2025.101543] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 09/19/2024] [Revised: 01/21/2025] [Accepted: 02/01/2025] [Indexed: 03/05/2025] Open
Abstract
The repair and functional recovery of long-segment peripheral nerve injuries are crucial in clinical settings. Nerve conduits are seen as promising alternatives to autologous nerve grafts, but their effectiveness is limited by the controlled delivery of bioactive factors and meeting various functional requirements during different stages of repair. This research developed multifunctional nerve conduits using electrospinning and polydopamine (PDA) coating techniques to integrate bioactive substances. Chitosan-composite PLGA electrospun nerve conduits demonstrated exceptional mechanical properties and biocompatibility. Nanofibers with specific topological structures effectively promoted oriented cell growth. The PDA coating provided ROS scavenging and immune modulation functions. The bFGF growth factor attached to the PDA coating facilitated sustained release, enhancing Schwann cell functionality and stimulating neurite outgrowth. In a rat sciatic nerve defect model with a 10 mm gap, PLGA/CS-PDA-bFGF nerve conduits showed a positive impact on nerve regeneration and functional recovery. Consequently, nerve conduits with multiple functions modified with PDA-coated bioactive molecules are poised to be excellent materials for mending peripheral nerve injuries.
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Affiliation(s)
- Xiaokun Chen
- Ninth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, China
| | - Jihai Xu
- Department of Hand Surgery, Department of Plastic Reconstructive Surgery, Ningbo No.6 Hospital, Ningbo, 315040, China
| | - Feng Qin
- Key Laboratory of Trauma and Neural Regeneration, Ministry of Education, Peking University, Beijing, 100044, China
- National Center for Trauma Medicine, Beijing, 100044, China
- Department of Orthopedics and Trauma, Peking University People's Hospital, Beijing, 100044, China
| | - Ziyuan Yang
- Key Laboratory of Trauma and Neural Regeneration, Ministry of Education, Peking University, Beijing, 100044, China
- National Center for Trauma Medicine, Beijing, 100044, China
- Trauma Medicine Center, Peking University People's Hospital, Beijing, 100044, China
| | - Xueyuan Li
- Department of Hand Surgery, Ningbo No.6 Hospital, Ningbo, 315040, China
| | - Miao Yu
- Department of Hand Surgery, Ningbo No.6 Hospital, Ningbo, 315040, China
| | - Ming Li
- Key Laboratory of Trauma and Neural Regeneration, Ministry of Education, Peking University, Beijing, 100044, China
- National Center for Trauma Medicine, Beijing, 100044, China
- Trauma Medicine Center, Peking University People's Hospital, Beijing, 100044, China
| | - Yanhua Wang
- Key Laboratory of Trauma and Neural Regeneration, Ministry of Education, Peking University, Beijing, 100044, China
- National Center for Trauma Medicine, Beijing, 100044, China
- Department of Orthopedics and Trauma, Peking University People's Hospital, Beijing, 100044, China
| | - Wang Xin
- Department of Plastic Reconstructive Surgery, Ningbo No.6 Hospital, Ningbo, 315040, China
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4
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Yokomine S, Makino T, Nagao E, Nakazawa K. Floating or adherent hepatocyte spheroid cultures using microwell chips with polyethylene glycol or polyimide surfaces. Biomed Mater 2025; 20:035009. [PMID: 40096815 DOI: 10.1088/1748-605x/adc17d] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 09/16/2024] [Accepted: 03/17/2025] [Indexed: 03/19/2025]
Abstract
Microwell chip culture is a promising technique for controlling spheroid size and producing a large number of homogeneous spheroids. In this study, we focused on the relationship between chip material and the properties of hepatocyte spheroids. The basic chip design was 397 circular microwells, each 400 µm in diameter. Two types of microwell chips were fabricated, coating the bottom surface either with polyethylene glycol (PEG chip) or polyimide (PI chip). Hepatocytes gradually aggregated and formed floating spheroids within each microwell in the PEG chip but formed adherent spheroids within each microwell of the PI chip. Such floating and adherent spheroid morphologies were maintained for at least one month of culture. An explanation for the spheroid formation mechanism is that the plasminogen activator (PA) /plasmin and matrix degradation/remodeling systems were activated in the formation of adherent spheroids. Furthermore, in adherent spheroid cultures, the formation of cell-matrix junctions was promoted, in addition to the development of intercellular junctions. The albumin secretion and drug metabolism activities of the hepatocyte spheroids were higher than those of traditional monolayer hepatocytes, and the adherent spheroids in the PI chip maintained a higher functional expression than the floating spheroids in the PEG chip. Further to this, functional properties of hepatocytes, the expressions of key metabolic enzymes, glucose 6-phosphatase (sugar metabolism), tryptophan 2, 3-dioxygenase (amino acid metabolism), arginase 1 (urea cycle), cytochrome P450 7a1 (lipid metabolism), and cytochrome P450 families (drug metabolism) were evaluated by gene expression analysis. The expression of these key enzymes in hepatocytes was higher in spheroid culture than in general monolayer culture, and the functions of adherent spheroids were superior to those of floating spheroids. These results indicate that the material properties of the microwell chips are important factors that regulate the morphological and functional characteristics of hepatocyte spheroids.
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Affiliation(s)
- Sae Yokomine
- Department of Life and Environment Engineering, The University of Kitakyushu , 1-1 Hibikino, Wakamatsu-ku, Kitakyushu, Fukuoka 808-0135, Japan
| | - Tomomi Makino
- NIPPON SHOKUBAI CO. , LTD, 5-8 Nishi Otabi-cho, Suita, Osaka, 564-0034, Japan
| | - Emiko Nagao
- NIPPON SHOKUBAI CO. , LTD, 5-8 Nishi Otabi-cho, Suita, Osaka, 564-0034, Japan
| | - Kohji Nakazawa
- Department of Life and Environment Engineering, The University of Kitakyushu , 1-1 Hibikino, Wakamatsu-ku, Kitakyushu, Fukuoka 808-0135, Japan
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5
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Ziąbka M, Wojteczko A, Zagrajczuk B, Benko A, Komarek S, Menaszek E. Biological evaluation of ZrO 2 composites modified with different ceramics additives. ARTIFICIAL CELLS, NANOMEDICINE, AND BIOTECHNOLOGY 2024; 52:551-563. [PMID: 39494713 DOI: 10.1080/21691401.2024.2422870] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 06/19/2024] [Revised: 10/15/2024] [Accepted: 10/21/2024] [Indexed: 11/05/2024]
Abstract
In this work, zirconia (ZrO2) composites modified with bioactive hydroxyapatite (HAp), hexagonal boron nitride (hBN), bioglass (BG), and bioglass with copper (BGCu) via the hydrothermal method were synthesized. The aim was to obtain highly bioactive and cytocompatible materials that could combine beneficial properties of inert and bioactive ceramics. Such materials could be applied as fillers for tooth extraction cavities, guaranteeing osseintegration without the need to introduce additional bone cements or other adhesives. It was proven that while all materials were favourable towards cells adhesion and growth, the HAp and BG-doped ones facilitated early adhesion, especially when compared to unmodified ZrO2. Only the HAp-doped materials showed satisfactory bioactivity results, with a well-developed apatite layer forming on their surfaces. This study confirms that the Hap-doped ZrO2 is suitable for treating bone defects.
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Affiliation(s)
- Magdalena Ziąbka
- Department of Ceramics and Refractories, Faculty of Materials Science and Ceramics, AGH University of Krakow, Krakow, Poland
| | - Agnieszka Wojteczko
- Department of Ceramics and Refractories, Faculty of Materials Science and Ceramics, AGH University of Krakow, Krakow, Poland
| | - Barbara Zagrajczuk
- Department of Glass Technology and Amorphous Coatings, Faculty of Materials Science and Ceramics, AGH University of Krakow, Krakow, Poland
| | - Aleksandra Benko
- Department of Biomaterials and Composites, Faculty of Materials Science and Ceramics, AGH University of Krakow, Krakow, Poland
| | - Sebastian Komarek
- Department of Ceramics and Refractories, Faculty of Materials Science and Ceramics, AGH University of Krakow, Krakow, Poland
| | - Elżbieta Menaszek
- Department of Cytobiology, Faculty of Pharmacy, Jagiellonian University Medical College, Krakow, Poland
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6
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Yoon JP, Kim H, Park SJ, Kim DH, Kim JY, Kim DH, Chung SW. Nanofiber Graft Therapy to Prevent Shoulder Stiffness and Adhesions after Rotator Cuff Tendon Repair: A Comprehensive Review. Biomedicines 2024; 12:1613. [PMID: 39062186 PMCID: PMC11274509 DOI: 10.3390/biomedicines12071613] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 05/20/2024] [Revised: 07/09/2024] [Accepted: 07/17/2024] [Indexed: 07/28/2024] Open
Abstract
Stiffness and adhesions following rotator cuff tears (RCTs) are common complications that negatively affect surgical outcomes and impede healing, thereby increasing the risk of morbidity and failure of surgical interventions. Tissue engineering, particularly through the use of nanofiber scaffolds, has emerged as a promising regenerative medicine strategy to address these complications. This review critically assesses the efficacy and limitations of nanofiber-based methods in promoting rotator cuff (RC) regeneration and managing postrepair stiffness and adhesions. It also discusses the need for a multidisciplinary approach to advance this field and highlights important considerations for future clinical trials.
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Affiliation(s)
- Jong Pil Yoon
- Department of Orthopedic Surgery, School of Medicine, Kyungpook National University, Daegu 41944, Republic of Korea; (J.P.Y.); (S.-J.P.); (D.-H.K.)
| | - Hyunjin Kim
- Department of Orthopedic Surgery, School of Medicine, Kyungpook National University, Daegu 41944, Republic of Korea; (J.P.Y.); (S.-J.P.); (D.-H.K.)
| | - Sung-Jin Park
- Department of Orthopedic Surgery, School of Medicine, Kyungpook National University, Daegu 41944, Republic of Korea; (J.P.Y.); (S.-J.P.); (D.-H.K.)
| | - Dong-Hyun Kim
- Department of Orthopedic Surgery, School of Medicine, Kyungpook National University, Daegu 41944, Republic of Korea; (J.P.Y.); (S.-J.P.); (D.-H.K.)
| | - Jun-Young Kim
- Department of Orthopedic Surgery, School of Medicine, Catholic University, Daegu 38430, Republic of Korea;
| | - Du Han Kim
- Department of Orthopedic Surgery, Keimyung University Dongsan Hospital, Keimyung University School of Medicine, Daegu 42601, Republic of Korea;
| | - Seok Won Chung
- Department of Orthopedic Surgery, Konkuk University Medical Center, Seoul 05030, Republic of Korea;
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7
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Xie Y, Liu W, Yang Y, Shi M, Li J, Sun Y, Wang Y, Zhang J, Zheng Y. Fabrication of a modified bacterial cellulose with different alkyl chains and its prevention of abdominal adhesion. Int J Biol Macromol 2024; 273:133191. [PMID: 38880455 DOI: 10.1016/j.ijbiomac.2024.133191] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 08/11/2023] [Revised: 06/12/2024] [Accepted: 06/13/2024] [Indexed: 06/18/2024]
Abstract
Abdominal hernia mesh is a common product which is used for prevention of abdominal adhesion and repairing abdominal wall defect. Currently, designing and preparing a novel bio-mesh material with prevention of adhesion, promoting repair and good biocompatibility simultaneously remain a great bottleneck. In this study, a novel siloxane-modified bacterial cellulose (BC) was designed and fabricated by chemical vapor deposition silylation, then the effects of different alkyl chains length of siloxane on surface properties and cell behaviors were explored. The effect of preventing of abdominal adhesion and repairing abdominal wall defect in rats with the siloxane-modified BC was evaluated. As the grafted alkyl chains become longer, the surface of the siloxane-modified BC can be transformed from super hydrophilic to hydrophobic. In vivo results showed that BC-C16 had good long-term anti-adhesion effect, good tissue adaptability and histocompatibility, which is expected to be used as a new anti-adhesion hernia repair material in clinic.
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Affiliation(s)
- Yajie Xie
- School of Material Science and Engineering, University of Science and Technology Beijing, Beijing 100083, China
| | - Wenbo Liu
- School of Material Science and Engineering, University of Science and Technology Beijing, Beijing 100083, China
| | - Yingying Yang
- School of Material Science and Engineering, University of Science and Technology Beijing, Beijing 100083, China
| | - Miaojie Shi
- School of Material Science and Engineering, University of Science and Technology Beijing, Beijing 100083, China
| | - Junfei Li
- School of Material Science and Engineering, University of Science and Technology Beijing, Beijing 100083, China
| | - Yi Sun
- School of Material Science and Engineering, University of Science and Technology Beijing, Beijing 100083, China
| | - Yansen Wang
- School of Material Science and Engineering, University of Science and Technology Beijing, Beijing 100083, China
| | - Jian Zhang
- Shanghai Changzheng Hospital, 415 Fengyang Street, Shanghai 200003, China.
| | - Yudong Zheng
- School of Material Science and Engineering, University of Science and Technology Beijing, Beijing 100083, China.
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8
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Sturgill B, Hernandez-Reynoso AG, Druschel LN, Smith TJ, Boucher PE, Hoeferlin GF, Thai TTD, Jiang MS, Hess JL, Alam NN, Menendez DM, Duncan JL, Cogan SF, Pancrazio JJ, Capadona JR. Reactive Amine Functionalized Microelectrode Arrays Provide Short-Term Benefit but Long-Term Detriment to In Vivo Recording Performance. ACS APPLIED BIO MATERIALS 2024; 7:1052-1063. [PMID: 38290529 PMCID: PMC10880090 DOI: 10.1021/acsabm.3c01014] [Citation(s) in RCA: 1] [Impact Index Per Article: 1.0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Grants] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 10/28/2023] [Revised: 01/08/2024] [Accepted: 01/10/2024] [Indexed: 02/01/2024]
Abstract
Intracortical microelectrode arrays (MEAs) are used for recording neural signals. However, indwelling devices result in chronic neuroinflammation, which leads to decreased recording performance through degradation of the device and surrounding tissue. Coating the MEAs with bioactive molecules is being explored to mitigate neuroinflammation. Such approaches often require an intermediate functionalization step such as (3-aminopropyl)triethoxysilane (APTES), which serves as a linker. However, the standalone effect of this intermediate step has not been previously characterized. Here, we investigated the effect of coating MEAs with APTES by comparing APTES-coated to uncoated controls in vivo and ex vivo. First, we measured water contact angles between silicon uncoated and APTES-coated substrates to verify the hydrophilic characteristics of the APTES coating. Next, we implanted MEAs in the motor cortex (M1) of Sprague-Dawley rats with uncoated or APTES-coated devices. We assessed changes in the electrochemical impedance and neural recording performance over a chronic implantation period of 16 weeks. Additionally, histology and bulk gene expression were analyzed to understand further the reactive tissue changes arising from the coating. Results showed that APTES increased the hydrophilicity of the devices and decreased electrochemical impedance at 1 kHz. APTES coatings proved detrimental to the recording performance, as shown by a constant decay up to 16 weeks postimplantation. Bulk gene analysis showed differential changes in gene expression between groups that were inconclusive with regard to the long-term effect on neuronal tissue. Together, these results suggest that APTES coatings are ultimately detrimental to chronic neural recordings. Furthermore, interpretations of studies using APTES as a functionalization step should consider the potential consequences if the final functionalization step is incomplete.
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Affiliation(s)
- Brandon
S. Sturgill
- Department
of Bioengineering, The University of Texas
at Dallas, 800 W. Campbell Road, Richardson, Texas 75080, United States
| | - Ana G. Hernandez-Reynoso
- Department
of Bioengineering, The University of Texas
at Dallas, 800 W. Campbell Road, Richardson, Texas 75080, United States
| | - Lindsey N. Druschel
- Department
of Biomedical Engineering, Case Western
Reserve University. 10900 Euclid Ave, Cleveland, Ohio 44106, United States
- Advanced
Platform Technology Center, Louis Stokes Cleveland Veterans Affairs
Medical Center, Cleveland, Ohio 44106, United States
| | - Thomas J. Smith
- School
of Behavioral and BrainSciences, The University
of Texas at Dallas, 800 W. Campbell Road, Richardson, Texas 75080, United States
| | - Pierce E. Boucher
- Department
of Biomedical Engineering, Case Western
Reserve University. 10900 Euclid Ave, Cleveland, Ohio 44106, United States
- Advanced
Platform Technology Center, Louis Stokes Cleveland Veterans Affairs
Medical Center, Cleveland, Ohio 44106, United States
| | - George F. Hoeferlin
- Department
of Biomedical Engineering, Case Western
Reserve University. 10900 Euclid Ave, Cleveland, Ohio 44106, United States
- Advanced
Platform Technology Center, Louis Stokes Cleveland Veterans Affairs
Medical Center, Cleveland, Ohio 44106, United States
| | - Teresa Thuc Doan Thai
- Department
of Bioengineering, The University of Texas
at Dallas, 800 W. Campbell Road, Richardson, Texas 75080, United States
| | - Madison S. Jiang
- School
of Behavioral and BrainSciences, The University
of Texas at Dallas, 800 W. Campbell Road, Richardson, Texas 75080, United States
| | - Jordan L. Hess
- School
of Behavioral and BrainSciences, The University
of Texas at Dallas, 800 W. Campbell Road, Richardson, Texas 75080, United States
| | - Neeha N. Alam
- Department
of Bioengineering, The University of Texas
at Dallas, 800 W. Campbell Road, Richardson, Texas 75080, United States
| | - Dhariyat M. Menendez
- Department
of Biomedical Engineering, Case Western
Reserve University. 10900 Euclid Ave, Cleveland, Ohio 44106, United States
- Advanced
Platform Technology Center, Louis Stokes Cleveland Veterans Affairs
Medical Center, Cleveland, Ohio 44106, United States
| | - Jonathan L. Duncan
- Department
of Biomedical Engineering, Case Western
Reserve University. 10900 Euclid Ave, Cleveland, Ohio 44106, United States
- Advanced
Platform Technology Center, Louis Stokes Cleveland Veterans Affairs
Medical Center, Cleveland, Ohio 44106, United States
| | - Stuart F. Cogan
- Department
of Bioengineering, The University of Texas
at Dallas, 800 W. Campbell Road, Richardson, Texas 75080, United States
| | - Joseph J. Pancrazio
- Department
of Bioengineering, The University of Texas
at Dallas, 800 W. Campbell Road, Richardson, Texas 75080, United States
| | - Jeffrey R. Capadona
- Department
of Biomedical Engineering, Case Western
Reserve University. 10900 Euclid Ave, Cleveland, Ohio 44106, United States
- Advanced
Platform Technology Center, Louis Stokes Cleveland Veterans Affairs
Medical Center, Cleveland, Ohio 44106, United States
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9
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Yeo RJ, Bleich JN, Guérin M, Morganella D, Berner M, Frauenrath H. Multifunctional Aluminum Pre-treatments from End-Functionalized Phosphonic Acid Self-Assembled Monolayers. LANGMUIR : THE ACS JOURNAL OF SURFACES AND COLLOIDS 2024. [PMID: 38306705 DOI: 10.1021/acs.langmuir.3c02629] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 02/04/2024]
Abstract
Aluminum alloys are used in advanced engineering applications as they possess a combination of favorable properties, including high strength, lightweightness, good corrosion resistance, machineability, and recyclability. Such applications often require forming the sheets into the final components, which is typically aided by an oil-based lubricant, followed by joining them using adhesives, which is hampered by residual lubricant. In this work, aluminum surfaces were modified with different self-assembled monolayers (SAMs), with the goal of significantly reducing the amount of lubricant while simultaneously improving friction properties, forming, and bonding performance. Our results show that SAMs terminated with hydrophilic and nucleophilic end groups give rise to high-energy surfaces with wetting properties that are stable over time. These surfaces showed significantly improved surface wetting by the lubricant, which in turn resulted in an improved forming performance at reduced lubricant coat weights. Moreover, the nucleophilic SAM termination provided outstanding performance in adhesive bonding tests under corrosive conditions.
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Affiliation(s)
- Reuben J Yeo
- Ecole Polytechnique Fédérale de Lausanne (EPFL) Institute of Materials, Lausanne 1015, Switzerland
| | - Julian N Bleich
- Ecole Polytechnique Fédérale de Lausanne (EPFL) Institute of Materials, Lausanne 1015, Switzerland
- Novelis SA Switzerland, Sierre 3900, Switzerland
| | | | | | | | - Holger Frauenrath
- Ecole Polytechnique Fédérale de Lausanne (EPFL) Institute of Materials, Lausanne 1015, Switzerland
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10
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Ramachandran B, Sabbatier G, Bowden OM, Campbell K, Fekete N, Girard-Lauriault PL, Hoesli CA. Human mesenchymal stromal cell adhesion and expansion on fluoropolymer surfaces modified with oxygen and nitrogen-rich plasma polymers. Colloids Surf B Biointerfaces 2024; 234:113740. [PMID: 38199188 DOI: 10.1016/j.colsurfb.2023.113740] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 09/25/2023] [Revised: 12/12/2023] [Accepted: 12/28/2023] [Indexed: 01/12/2024]
Abstract
Fluorinated ethylene propylene (FEP) vessels are of significant interest for therapeutic cell biomanufacturing applications due to their chemical inertness, hydrophobic surface, and high oxygen permeability. However, these properties also limit the adhesion and survival of anchorage-dependent cells. Here, we develop novel plasma polymer coatings to modify FEP surfaces, enhancing the adhesion and expansion of human mesenchymal stromal cells (hMSCs). Similar to commercially available tissue culture polystyrene vessels, oxygen-rich or nitrogen-rich surface chemistries can be achieved using this approach. While steam sterilization increased the roughness of the coatings and altered the surface chemistry, the overall wettability and oxygen or nitrogen-rich nature of the coatings were maintained. In the absence of proteins during initial cell attachment, cells adhered to surfaces even in the presence of chelators, whereas adhesion was abrogated with chelator in a protein-containing medium, suggesting that integrin-mediated adhesion predominates over physicochemical tethering in normal protein-containing cell seeding conditions. Albumin adsorption was more elevated on nitrogen-rich coatings compared to the oxygen-rich coatings, which was correlated with a higher extent of hMSC expansion after 3 days. Both the oxygen and nitrogen-rich coatings significantly improved hMSC adhesion and expansion compared to untreated FEP. FEP surfaces with nitrogen-rich coatings were practically equivalent to commercially available standard tissue culture-treated polystyrene surfaces in terms of hMSC yields. Plasma polymer coatings show significant promise in expanding the potential usage of FEP-based culture vessels for cell therapy applications.
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Affiliation(s)
| | - Gad Sabbatier
- Department of Chemical Engineering, McGill University, Montréal, Canada
| | - Olivia M Bowden
- Department of Chemical Engineering, McGill University, Montréal, Canada
| | - Katie Campbell
- Saint-Gobain Ceramics & Plastics, Inc., Northboro R&D Center, Northborough, MA, USA
| | - Natalie Fekete
- Saint-Gobain Ceramics & Plastics, Inc., Northboro R&D Center, Northborough, MA, USA
| | | | - Corinne A Hoesli
- Department of Chemical Engineering, McGill University, Montréal, Canada.
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11
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Baheti W, Chen X, La M, He H. Biomimetic HA-GO implant coating for enhanced osseointegration via macrophage M2 polarization-induced osteo-immunomodulation. J Appl Biomater Funct Mater 2024; 22:22808000241266665. [PMID: 39129373 DOI: 10.1177/22808000241266665] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 08/13/2024] Open
Abstract
The pro-inflammatory/anti-inflammatory polarized phenotypes of macrophages (M1/M2) can be used to predict the success of implant integration. Hence, activating and inducing the transformation of immunocytes that promote tissue repair appears to be a highly promising strategy for facilitating osteo-anagenesis. In a previous study, titanium implants were coated with a graphene oxide-hydroxyapatite (GO-HA) nanocomposite via electrophoretic deposition, and the osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) was found to be significantly enhanced when the GO content was 2wt%. However, the effectiveness of the GO-HA nanocomposite coating in modifying the in vivo immune microenvironment still remains unclear. In this study, the effects of GO-HA coatings on osteogenesis were investigated based on the GO-HA-mediated immune regulation of macrophages. The HA-2wt%GO nanocomposite coatings exhibited good biocompatibility and favored M2 macrophage polarization. Meanwhile, they could also significantly upregulate IL-10 (anti-inflammatory factor) expression and downregulate TNF-α (pro-inflammatory factor) expression. Additionally, the microenvironment, which was established by M2 macrophages, favored the osteogenesis of BMSCs both in vivo and in vitro. These findings show that the GO-HA nanocomposite coating is a promising surface-modification material. Hence, this study provides a reference for the development of next-generation osteoimmunomodulatory biomaterials.
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Affiliation(s)
- Wufanbieke Baheti
- Department of Stomatology, People's Hospital of Xinjiang Autonomous Region, Urumqi, China
- The Affiliated Hospital of Stomatology, School of Stomatology, Zhejiang University School of Medicine, Hangzhou, China
| | - Xiaotao Chen
- Department of Stomatology, People's Hospital of Xinjiang Autonomous Region, Urumqi, China
| | - Mi La
- Department of Stomatology, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, China
| | - Huiyu He
- Department of Stomatology, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, China
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12
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Wang MT, Pang SW. Enhancing Nasopharyngeal Carcinoma Cell Separation with Selective Fibronectin Coating and Topographical Modification on Polydimethylsiloxane Scaffold Platforms. Int J Mol Sci 2023; 24:12409. [PMID: 37569784 PMCID: PMC10418797 DOI: 10.3390/ijms241512409] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Grants] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 07/06/2023] [Revised: 07/29/2023] [Accepted: 08/02/2023] [Indexed: 08/13/2023] Open
Abstract
The extracellular matrix (ECM) serves as a complex scaffold with diverse physical dimensions and surface properties influencing NPC cell migration. Polydimethylsiloxane (PDMS), a widely used biocompatible material, is hydrophobic and undesirable for cell seeding. Thus, the establishment of a biomimetic model with varied topographies and surface properties is essential for effective NPC43 cell separation from NP460 cells. This study explored how ECM surface properties influence NP460 and NPC43 cell behaviors via plasma treatments and chemical modifications to alter the platform surface. In addition to the conventional oxygen/nitrogen (O2/N2) plasma treatment, O2 and argon plasma treatments were utilized to modify the platform surface, which increased the hydrophilicity of the PDMS platforms, resulting in enhanced cell adhesion. (3-aminopropyl)triethoxysilane and fibronectin (FN) were used to coat the PDMS platforms uniformly and selectively. The chemical coatings significantly affected cell motility and spreading, as cells exhibited faster migration, elongated cell shapes, and larger spreading areas on FN-coated surfaces. Furthermore, narrower top layer trenches with 5 µm width and a lower concentration of 10 µg/mL FN were coated selectively on the platforms to limit NP460 cell movements and enhance NPC43 cell separation efficiency. A significantly high separation efficiency of 99.4% was achieved on the two-layer scaffold platform with 20/5 µm wide ridge/trench (R/T) as the top layer and 40/10 µm wide R/T as the bottom layer, coupling with 10 µg/mL FN selectively coated on the sidewalls of the top and bottom layers. This work demonstrated an innovative application of selective FN coating to direct cell behavior, offering a new perspective to probe into the subtleties of NPC cell separation efficiency. Moreover, this cost-effective and compact microsystem sets a new benchmark for separating cancer cells.
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Affiliation(s)
| | - S. W. Pang
- Department of Electrical Engineering, Centre for Biosystems, Neuroscience, and Nanotechnology, City University of Hong Kong, Hong Kong 999077, China;
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13
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Durdu S, Cihan G, Yalcin E, Cavusoglu K, Altinkok A, Sagcan H, Yurtsever İ, Usta M. Surface characterization, electrochemical properties and in vitro biological properties of Zn-deposited TiO 2 nanotube surfaces. Sci Rep 2023; 13:11423. [PMID: 37452093 PMCID: PMC10349054 DOI: 10.1038/s41598-023-38733-2] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 01/31/2023] [Accepted: 07/13/2023] [Indexed: 07/18/2023] Open
Abstract
In this work, to improve antibacterial, biocompatible and bioactive properties of commercial pure titanium (cp-Ti) for implant applications, the Zn-deposited nanotube surfaces were fabricated on cp-Ti by using combined anodic oxidation (AO) and physical vapor deposition (PVD-TE) methods. Homogenous elemental distributions were observed through all surfaces. Moreover, Zn-deposited surfaces exhibited hydrophobic character while bare Ti surfaces were hydrophilic. Due to the biodegradable behavior of Zn on the nanotube surface, Zn-deposited nanotube surfaces showed higher corrosion current density than bare cp-Ti surface in SBF conditions as expected. In vitro biological properties such as cell viability, ALP activity, protein adsorption, hemolytic activity and antibacterial activity for Gram-positive and Gram-negative bacteria of all surfaces were investigated in detail. Cell viability, ALP activity and antibacterial properties of Zn-deposited nanotube surfaces were significantly improved with respect to bare cp-Ti. Moreover, hemolytic activity and protein adsorption of Zn-deposited nanotube surfaces were decreased. According to these results; a bioactive, biocompatible and antibacterial Zn-deposited nanotube surfaces produced on cp-Ti by using combined AO and PVD techniques can have potential for orthopedic and dental implant applications.
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Affiliation(s)
- Salih Durdu
- Industrial Engineering, Faculty of Engineering, Giresun University, Merkez, 28200, Giresun, Turkey.
- Mechanical Engineering, Giresun University, 28200, Giresun, Turkey.
| | - Gizem Cihan
- Department of Biology, Giresun University, 28200, Giresun, Turkey
| | - Emine Yalcin
- Department of Biology, Giresun University, 28200, Giresun, Turkey
| | | | - Atilgan Altinkok
- Turkish Naval Academy, National Defence University, 34940, Istanbul, Turkey
| | - Hasan Sagcan
- Department of Medical Laboratory Techniques, Istanbul Medipol University, Istanbul, Turkey
| | - İlknur Yurtsever
- Department of Medical Laboratory Techniques, Istanbul Medipol University, Istanbul, Turkey
- Pharmacology and Toxicology Department, Boonshoft School of Medicine Ohio, Wright State University, Dayton, USA
| | - Metin Usta
- Materials Science and Engineering, Gebze Technical University, 41400, Gebze, Turkey
- Aluminum Research Center (GTU-AAUM), Gebze Technical University, 41400, Gebze, Turkey
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14
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van Dalen M, Karperien M, Claessens MM, Post JN. Choice of Protein, Not Its Amyloid-Fold, Determines the Success of Amyloid-Based Scaffolds for Cartilage Tissue Regeneration. ACS OMEGA 2023; 8:24198-24209. [PMID: 37457450 PMCID: PMC10339334 DOI: 10.1021/acsomega.3c00151] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Grants] [Track Full Text] [Download PDF] [Figures] [Subscribe] [Scholar Register] [Received: 01/09/2023] [Accepted: 05/18/2023] [Indexed: 07/18/2023]
Abstract
The formation of fibrocartilage during articular cartilage regeneration remains a clinical problem affecting adequate restoration of articular cartilage in joints. To stimulate chondrocytes to form articular cartilage, we investigated the use of amyloid fibril-based scaffolds. The proteins α-synuclein, β-lactoglobulin, and lysozyme were induced to self-assemble into amyloid fibrils and, during dialysis, formed micrometer scale amyloid networks that resemble the cartilage extracellular matrix. Our results show that lysozyme amyloid micronetworks supported chondrocyte viability and extracellular matrix deposition, while α-synuclein and β-lactoglobulin maintained cell viability. With this study, we not only confirm the possible use of amyloid materials for tissue regeneration but also demonstrate that the choice of protein, rather than its amyloid-fold per se, affects the cellular response and tissue formation.
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Affiliation(s)
- Maurice
C.E. van Dalen
- Developmental
BioEngineering, TechMed Centre, University
of Twente, Enschede, Overijssel 7500 AE, The Netherlands
- Nanobiophysics,
Mesa+, University of Twente, Enschede 7500AE, The Netherlands
| | - Marcel Karperien
- Developmental
BioEngineering, TechMed Centre, University
of Twente, Enschede, Overijssel 7500 AE, The Netherlands
| | | | - Janine N. Post
- Developmental
BioEngineering, TechMed Centre, University
of Twente, Enschede, Overijssel 7500 AE, The Netherlands
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15
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Cobeña-Reyes J, Ye T, Martini A. Simulations of Subnanometer Scale Image Contrast in Atomic Force Microscopy of Self-Assembled Monolayers in Water. CHEMICAL & BIOMEDICAL IMAGING 2023; 1:147-156. [PMID: 37235190 PMCID: PMC10208375 DOI: 10.1021/cbmi.3c00001] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Grants] [Track Full Text] [Download PDF] [Figures] [Subscribe] [Scholar Register] [Received: 01/03/2023] [Revised: 02/10/2023] [Accepted: 02/20/2023] [Indexed: 05/28/2023]
Abstract
Achieving high-resolution images using dynamic atomic force microscopy (AFM) requires understanding how chemical and structural features of the surface affect image contrast. This understanding is particularly challenging when imaging samples in water. An initial step is to determine how well-characterized surface features interact with the AFM tip in wet environments. Here, we use molecular dynamics simulations of a model AFM tip apex oscillating in water above self-assembled monolayers (SAMs) with different chain lengths and functional groups. The amplitude response of the tip is characterized across a range of vertical distances and amplitude set points. Then relative image contrast is quantified as the difference of the amplitude response of the tip when it is positioned directly above a SAM functional group vs positioned between two functional groups. Differences in contrast between SAMs with different lengths and functional groups are explained in terms of the vertical deflection of the SAMs due to interactions with the tip and water during dynamic imaging. The knowledge gained from simulations of these simple model systems may ultimately be used to guide selection of imaging parameters for more complex surfaces.
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Affiliation(s)
- José Cobeña-Reyes
- Department
of Mechanical Engineering, University of
California Merced, Merced, California 95343, United States
| | - Tao Ye
- Department
of Chemistry & Biochemistry, University
of California Merced, Merced, California 95343, United States
| | - Ashlie Martini
- Department
of Mechanical Engineering, University of
California Merced, Merced, California 95343, United States
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16
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Zhang Y, Yang C, Yin S, Zhang X, Peng X, Li G. Exploration of 2D and 2.5D Conformational Designs Applied on Epoxide/Collagen-Based Integrative Biointerfaces with Device/Tissue Heterogeneous Affinity. ACS APPLIED MATERIALS & INTERFACES 2023; 15:22876-22891. [PMID: 37144968 DOI: 10.1021/acsami.3c00945] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 05/06/2023]
Abstract
Collagen and multifunctional epoxides, which are respectively the common constituents of natural and polymer interfaces, were combined to fabricate integrative biointerfaces with device/tissue heterogeneous affinity. Further, the traditional 2D and advanced 2.5D conformational designs were achieved on collagen-based biointerfaces. The 2D conformational biointerfaces were formed by the self-entanglement of collagen molecules based on extensive hydrogen bonds among molecules, and the lamellar structures of 2D conformational biointerfaces could act as barriers to protect both biointerfaces and substrates from enzymes and corrosion. The unique stacking structures of 2.5D conformational biointerfaces were formed by cross-linking microaggregates that were established and connected by epoxy cross-linking bonds and provided the extra 0.5D degree of freedom on structure design and functional specialization through artificially manipulating the constituents and density of microaggregates. Besides, the intersecting channels among microaggregates gave 2.5D biointerfaces diffusion behaviors, which further brought good wettability and biodegradability. The integrative biointerfaces behaved well on cell viability and enhanced the cell adhesion strength in vitro, which could be attributed to the collaborations of collagen and epoxy groups. The subcutaneous implant model in rats was utilized to investigate soft tissue response, and the results demonstrated that the tissues around implantation areas healed well and without calcification or infection. The coating of integrative biointerfaces alleviated the fibrosis around implantation areas, and the inflammatory responses and foreign body reactions were improved.
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Affiliation(s)
- Yuanzhi Zhang
- National Engineering Laboratory for Clean Technology of Leather Manufacture, Sichuan University, Chengdu 610065, P.R. China
| | - Changkai Yang
- National Engineering Laboratory for Clean Technology of Leather Manufacture, Sichuan University, Chengdu 610065, P.R. China
| | - Simiao Yin
- National Engineering Laboratory for Clean Technology of Leather Manufacture, Sichuan University, Chengdu 610065, P.R. China
| | - Xiaoxia Zhang
- Key Laboratory of Leather Chemistry and Engineering (Sichuan University), Ministry of Education, Chengdu 610065, China
| | - Xu Peng
- Experimental and Research Animal Institute, Sichuan University, Chengdu 610065, China
| | - Guoying Li
- National Engineering Laboratory for Clean Technology of Leather Manufacture, Sichuan University, Chengdu 610065, P.R. China
- Key Laboratory of Leather Chemistry and Engineering (Sichuan University), Ministry of Education, Chengdu 610065, China
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17
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Wang X, Gu Z, Wan J, Zhou X, Zhu K, Wang X, Cao X, Yu X, Peng X, Tang Y. dECM based dusal-responsive vascular graft with enzyme-controlled adenine release for long-term patency. Int J Biol Macromol 2023; 242:124618. [PMID: 37148948 DOI: 10.1016/j.ijbiomac.2023.124618] [Citation(s) in RCA: 2] [Impact Index Per Article: 1.0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 11/19/2022] [Revised: 04/18/2023] [Accepted: 04/23/2023] [Indexed: 05/08/2023]
Abstract
Rapid occlusion is the culprit leading to implantation failure of biological blood vessels. Although adenosine is a clinical-proven drug to overcome the problem, its short half-life and turbulent burst-release limit its direct application. Thus, a pH/temperature dual-responsive blood vessel possessed controllable long-term adenosine secretion was constructed based on acellular matrix via compact crosslinking by oxidized chondroitin sulfate (OCSA) and functionalized with apyrase and acid phosphatase. These enzymes, as adenosine micro-generators, controlled the adenosine release amount by "real-time-responding" to acidity and temperature of vascular inflammation sites. Additionally, the macrophage phenotype was switched from M1 to M2, and related factors expression proved that adenosine release was effectively regulated with the severity of inflammation. What's more, the ultra-structure for degradation resisting and endothelialization accelerating was also preserved by their "double-crosslinking". Therefore, this work suggested a new feasible strategy providing a bright future of long-term patency for transplanted blood vessels.
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Affiliation(s)
- Xu Wang
- International Collaborative Centre on Big Science Plan for Purinergic Signaling, School of Acupuncture and Tuina, Chengdu University of Traditional Chinese Medicine, Chengdu 610075, China.
| | - Zhipeng Gu
- College of Polymer Science and Engineering, Sichuan University, Chengdu 610065, China
| | - Junyu Wan
- International Collaborative Centre on Big Science Plan for Purinergic Signaling, School of Acupuncture and Tuina, Chengdu University of Traditional Chinese Medicine, Chengdu 610075, China
| | - Xiong Zhou
- Department of Biomedical Engineering, City University of Hong Kong, 999077, Hong Kong, China
| | - Keli Zhu
- International Collaborative Centre on Big Science Plan for Purinergic Signaling, School of Acupuncture and Tuina, Chengdu University of Traditional Chinese Medicine, Chengdu 610075, China
| | - Xin Wang
- International Collaborative Centre on Big Science Plan for Purinergic Signaling, School of Acupuncture and Tuina, Chengdu University of Traditional Chinese Medicine, Chengdu 610075, China
| | - Xin Cao
- International Collaborative Centre on Big Science Plan for Purinergic Signaling, School of Acupuncture and Tuina, Chengdu University of Traditional Chinese Medicine, Chengdu 610075, China
| | - Xixun Yu
- College of Polymer Science and Engineering, Sichuan University, Chengdu 610065, China
| | - Xu Peng
- College of Polymer Science and Engineering, Sichuan University, Chengdu 610065, China.
| | - Yong Tang
- International Collaborative Centre on Big Science Plan for Purinergic Signaling, School of Acupuncture and Tuina, Chengdu University of Traditional Chinese Medicine, Chengdu 610075, China; Acupuncture & Chronobiology Key Laboratory of Sichuan Province, School of Health and Rehabilitation, Chengdu University of Traditional Chinese Medicine, Chengdu 610065, China.
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18
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Durdu S, Yalçin E, Altinkök A, Çavuşoğlu K. Characterization and investigation of electrochemical and biological properties of antibacterial silver nanoparticle-deposited TiO 2 nanotube array surfaces. Sci Rep 2023; 13:4699. [PMID: 36949171 PMCID: PMC10033515 DOI: 10.1038/s41598-023-31937-6] [Citation(s) in RCA: 6] [Impact Index Per Article: 3.0] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 02/16/2023] [Accepted: 03/20/2023] [Indexed: 03/24/2023] Open
Abstract
The one of main reasons of the premature failure of Ti-based implants is infections. The metal- and metal oxide-based nanoparticles have very high potential on controlling of infections. In this work, the randomly distributed AgNPs-deposited onto well-ordered TiO2 nanotube surfaces were fabricated on titanium by anodic oxidation (AO) and electrochemical deposition (ED) processes. AgNPs-deposited nanotube surfaces, which is beneficial for bone tissue growth exhibited hydrophilic behaviors. Moreover, the AgNPs-deposited nanotube surfaces, which prevent the leaching of metallic Ti ions from the implant surface, indicated great corrosion resistance under SBF conditions. The electrochemical corrosion resistance of AgNPs-deposited nanotube surfaces was improved up to about 145% compared to bare Gr2 surface. The cell viability of AgNPs-deposited nanotube surfaces was improved. Importantly, the AgNPs-deposited nanotube surfaces exhibited antibacterial activity for Gram-positive and Gram-negative bacteria. Eventually, it can be concluded that the AgNPs-deposited nanotube surfaces possess high stability for long-term usage of implant applications.
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Affiliation(s)
- Salih Durdu
- Industrial Engineering, Giresun University, Faculty of Engineering, 28200, Giresun, Turkey.
| | - Emine Yalçin
- Department of Biology, Giresun University, Faculty of Science, 28200, Giresun, Turkey
| | - Atilgan Altinkök
- Turkish Naval Academy, National Defence University, 34940, Istanbul, Turkey
| | - Kültiğin Çavuşoğlu
- Department of Biology, Giresun University, Faculty of Science, 28200, Giresun, Turkey.
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19
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Preparation and Surface Characterization of Chitosan-Based Coatings for PET Materials. Molecules 2023; 28:molecules28052375. [PMID: 36903621 PMCID: PMC10005435 DOI: 10.3390/molecules28052375] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 11/30/2022] [Revised: 02/18/2023] [Accepted: 02/20/2023] [Indexed: 03/08/2023] Open
Abstract
Poly(ethylene terephthalate)-PET-is one of the most frequently used polymers in biomedical applications. Due to chemical inertness, PET surface modification is necessary to gain specific properties, making the polymer biocompatible. The aim of this paper is to characterize the multi-component films containing chitosan (Ch), phospholipid 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC), immunosuppressant cyclosporine A (CsA) and/or antioxidant lauryl gallate (LG) which can be utilized as a very attractive material for developing the PET coatings. Chitosan was employed owing to its antibacterial activity and also its ability to promote cell adhesion and proliferation favorable for tissue engineering and regeneration purposes. Moreover, the Ch film can be additionally modified with other substances of biological importance (DOPC, CsA and LG). The layers of varying compositions were prepared using the Langmuir-Blodgett (LB) technique on the air plasma-activated PET support. Then their nanostructure, molecular distribution, surface chemistry and wettability were determined by atomic force microscopy (AFM), time-of-flight secondary ion mass spectrometry (TOF-SIMS), X-ray photoelectron spectroscopy (XPS), contact angle (CA) measurements and the surface free energy and its components' determination, respectively. The obtained results show clearly the dependence of the surface properties of the films on the molar ratio of components and allow for a better understanding of the coating organization and mechanisms of interactions at the molecular level both inside the films and between the films and the polar/apolar liquids imitating the environment of different properties. The organized layers of this type can be helpful in gaining control over the surface properties of the biomaterial, thus getting rid of the limitations in favor of increased biocompatibility. This is a good basis for further investigations on the correlation of the immune system response to the presence of biomaterial and its physicochemical properties.
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20
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Sahu MR, Sampath Kumar TS, Chakkingal U, Dewangan VK, Doble M. Influence of fine‐grained structure produced by groove pressing on the properties of pure Mg and commercial
ZE41
alloy. J Biomed Mater Res A 2023. [DOI: 10.1002/jbm.a.37502] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 02/16/2023]
Affiliation(s)
- Manas Ranjan Sahu
- Department of Metallurgical and Materials Engineering Indian Institute of Technology Madras Chennai India
| | - T. S. Sampath Kumar
- Department of Metallurgical and Materials Engineering Indian Institute of Technology Madras Chennai India
| | - Uday Chakkingal
- Department of Metallurgical and Materials Engineering Indian Institute of Technology Madras Chennai India
| | - Vimal Kumar Dewangan
- Department of Metallurgical and Materials Engineering Indian Institute of Technology Madras Chennai India
- Department of Biotechnology Indian Institute of Technology Madras Chennai India
| | - Mukesh Doble
- Department of Biotechnology Indian Institute of Technology Madras Chennai India
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21
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Husteden C, Brito Barrera YA, Tegtmeyer S, Borges J, Giselbrecht J, Menzel M, Langner A, Mano JF, Schmelzer CEH, Wölk C, Groth T. Lipoplex-Functionalized Thin-Film Surface Coating Based on Extracellular Matrix Components as Local Gene Delivery System to Control Osteogenic Stem Cell Differentiation. Adv Healthc Mater 2023; 12:e2201978. [PMID: 36377486 PMCID: PMC11469139 DOI: 10.1002/adhm.202201978] [Citation(s) in RCA: 4] [Impact Index Per Article: 2.0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Grants] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 08/05/2022] [Revised: 10/25/2022] [Indexed: 11/16/2022]
Abstract
A gene-activated surface coating is presented as a strategy to design smart biomaterials for bone tissue engineering. The thin-film coating is based on polyelectrolyte multilayers composed of collagen I and chondroitin sulfate, two main biopolymers of the bone extracellular matrix, which are fabricated by layer-by-layer assembly. For further functionalization, DNA/lipid-nanoparticles (lipoplexes) are incorporated into the multilayers. The polyelectrolyte multilayer fabrication and lipoplex deposition are analyzed by surface sensitive analytical methods that demonstrate successful thin-film formation, fibrillar structuring of collagen, and homogenous embedding of lipoplexes. Culture of mesenchymal stem cells on the lipoplex functionalized multilayer results in excellent attachment and growth of them, and also, their ability to take up cargo like fluorescence-labelled DNA from lipoplexes. The functionalization of the multilayer with lipoplexes encapsulating DNA encoding for transient expression of bone morphogenetic protein 2 induces osteogenic differentiation of mesenchymal stem cells, which is shown by mRNA quantification for osteogenic genes and histochemical staining. In summary, the novel gene-functionalized and extracellular matrix mimicking multilayer composed of collagen I, chondroitin sulfate, and lipoplexes, represents a smart surface functionalization that holds great promise for tissue engineering constructs and implant coatings to promote regeneration of bone and other tissues.
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Affiliation(s)
- Catharina Husteden
- Institute of PharmacyDepartment of Medicinal ChemistryMartin Luther University Halle‐WittenbergWolfgang‐Langenbeck‐Str. 406120Halle (Saale)Germany
| | - Yazmin A. Brito Barrera
- Institute of PharmacyDepartment of Biomedical MaterialsMartin Luther University Halle‐WittenbergHeinrich‐Damerow‐Str. 406120Halle (Saale)Germany
| | - Sophia Tegtmeyer
- Institute of PharmacyDepartment of Medicinal ChemistryMartin Luther University Halle‐WittenbergWolfgang‐Langenbeck‐Str. 406120Halle (Saale)Germany
| | - João Borges
- Department of ChemistryCICECO – Aveiro Institute of MaterialsUniversity of AveiroCampus Universitário de SantiagoAveiro3810‐193Portugal
| | - Julia Giselbrecht
- Institute of PharmacyDepartment of Medicinal ChemistryMartin Luther University Halle‐WittenbergWolfgang‐Langenbeck‐Str. 406120Halle (Saale)Germany
| | - Matthias Menzel
- Department of Biological and Macromolecular MaterialsFraunhofer Institute for Microstructure of Materials and Systems (IMWS)Walter‐Hülse‐Str. 106120Halle (Saale)Germany
| | - Andreas Langner
- Institute of PharmacyDepartment of Medicinal ChemistryMartin Luther University Halle‐WittenbergWolfgang‐Langenbeck‐Str. 406120Halle (Saale)Germany
| | - João F. Mano
- Department of ChemistryCICECO – Aveiro Institute of MaterialsUniversity of AveiroCampus Universitário de SantiagoAveiro3810‐193Portugal
| | - Christian E. H. Schmelzer
- Department of Biological and Macromolecular MaterialsFraunhofer Institute for Microstructure of Materials and Systems (IMWS)Walter‐Hülse‐Str. 106120Halle (Saale)Germany
| | - Christian Wölk
- Institute of PharmacyPharmaceutical TechnologyFaculty of MedicineLeipzig University04317LeipzigGermany
| | - Thomas Groth
- Institute of PharmacyDepartment of Biomedical MaterialsMartin Luther University Halle‐WittenbergHeinrich‐Damerow‐Str. 406120Halle (Saale)Germany
- Interdisciplinary Center of Materials ScienceMartin Luther University Halle‐WittenbergHeinrich‐Damerow‐Str. 406120Halle (Saale)Germany
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22
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Zeng K, Doberenz F, Lu YT, Nong JP, Fischer S, Groth T, Zhang K. Synthesis of Thermoresponsive PNIPAM-Grafted Cellulose Sulfates for Bioactive Multilayers via Layer-by-Layer Technique. ACS APPLIED MATERIALS & INTERFACES 2022; 14:48384-48396. [PMID: 36264178 DOI: 10.1021/acsami.2c12803] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.3] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 06/16/2023]
Abstract
The robust thermoresponsive and bioactive surfaces for tissue engineering by combining poly-N-isopropylacrylamide (PNIPAM) and cellulose sulfate (CS) remain highly in demand but not yet realized. Herein, PNIPAM-grafted cellulose sulfates (PCSs) with diverse degrees of substitution ascribed to sulfate groups (DSS) are synthesized for the first time. Higher sulfated PCS2 generally forms larger aggregates than lower sulfated PCS1 at their cloud point temperatures (TCP) of around 33 °C, whereas PCS1 leads to larger aggregates at body temperature (37 °C). Via the layer-by-layer (LbL) technique, biocompatible polyelectrolyte multilayers (PEMs) composed of PCSs as polyanions in combination with poly-l-lysine (PLL) or quaternized chitosan (QCHI) as polycations were fabricated. The resulting surfaces contained a more intermingled structure of polyanions with both polycations, while higher sulfated cellulose derivatives (CS2 and PCS2) displayed greater stability. Studies on toxicity and biocompatibility of PEM using 3T3 mouse fibroblasts showed a lower cytotoxicity of PEM with PCS2 and CS2 than PCS1 and CS1. Furthermore, the PEM using PCS2 particularly in combination with QCHI demonstrated excellent biocompatibility that is promising for new bioactive, thermoresponsive coatings on biomaterials and substrata for culturing adhesion-dependent cells.
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Affiliation(s)
- Kui Zeng
- Sustainable Materials and Chemistry, Department of Wood Technology and Wood-based Composites, University of Göttingen, Büsgenweg 4, Göttingen D-37077, Germany
| | - Falko Doberenz
- Department Biomedical Materials, Institute of Pharmacy, Martin Luther University Halle-Wittenberg, Heinrich-Damerow-Strasse 4, Halle (Saale) 06120, Germany
| | - Yi-Tung Lu
- Department Biomedical Materials, Institute of Pharmacy, Martin Luther University Halle-Wittenberg, Heinrich-Damerow-Strasse 4, Halle (Saale) 06120, Germany
| | - Johanna Phuong Nong
- Institute of Plant and Wood Chemistry (IPWC), Technische Universität Dresden, Pienner Straße 19, Tharandt 01737, Germany
| | - Steffen Fischer
- Institute of Plant and Wood Chemistry (IPWC), Technische Universität Dresden, Pienner Straße 19, Tharandt 01737, Germany
| | - Thomas Groth
- Department Biomedical Materials, Institute of Pharmacy, Martin Luther University Halle-Wittenberg, Heinrich-Damerow-Strasse 4, Halle (Saale) 06120, Germany
- Interdisciplinary Center of Material Science, Martin Luther University Halle-Wittenberg, Halle (Saale) 06099, Germany
| | - Kai Zhang
- Sustainable Materials and Chemistry, Department of Wood Technology and Wood-based Composites, University of Göttingen, Büsgenweg 4, Göttingen D-37077, Germany
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23
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Martínez-Miguel M, Castellote-Borrell M, Köber M, Kyvik AR, Tomsen-Melero J, Vargas-Nadal G, Muñoz J, Pulido D, Cristóbal-Lecina E, Passemard S, Royo M, Mas-Torrent M, Veciana J, Giannotti MI, Guasch J, Ventosa N, Ratera I. Hierarchical Quatsome-RGD Nanoarchitectonic Surfaces for Enhanced Integrin-Mediated Cell Adhesion. ACS APPLIED MATERIALS & INTERFACES 2022; 14:48179-48193. [PMID: 36251059 PMCID: PMC9614722 DOI: 10.1021/acsami.2c10497] [Citation(s) in RCA: 3] [Impact Index Per Article: 1.0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Grants] [Track Full Text] [Figures] [Subscribe] [Scholar Register] [Received: 06/13/2022] [Accepted: 10/05/2022] [Indexed: 06/16/2023]
Abstract
The synthesis and study of the tripeptide Arg-Gly-Asp (RGD), the binding site of different extracellular matrix proteins, e.g., fibronectin and vitronectin, has allowed the production of a wide range of cell adhesive surfaces. Although the surface density and spacing of the RGD peptide at the nanoscale have already shown a significant influence on cell adhesion, the impact of its hierarchical nanostructure is still rather unexplored. Accordingly, a versatile colloidal system named quatsomes, based on fluid nanovesicles formed by the self-assembling of cholesterol and surfactant molecules, has been devised as a novel template to achieve hierarchical nanostructures of the RGD peptide. To this end, RGD was anchored on the vesicle's fluid membrane of quatsomes, and the RGD-functionalized nanovesicles were covalently anchored to planar gold surfaces, forming a state of quasi-suspension, through a long poly(ethylene glycol) (PEG) chain with a thiol termination. An underlying self-assembled monolayer (SAM) of a shorter PEG was introduced for vesicle stabilization and to avoid unspecific cell adhesion. In comparison with substrates featuring a homogeneous distribution of RGD peptides, the resulting hierarchical nanoarchitectonic dramatically enhanced cell adhesion, despite lower overall RGD molecules on the surface. The new versatile platform was thoroughly characterized using a multitechnique approach, proving its enhanced performance. These findings open new methods for the hierarchical immobilization of biomolecules on surfaces using quatsomes as a robust and novel tissue engineering strategy.
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Affiliation(s)
- Marc Martínez-Miguel
- Institut
de Ciència de Materials de Barcelona (ICMAB-CSIC), Campus UAB, Bellaterra 08193, Spain
- Biomedical
Research Networking Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Madrid 28029, Spain
| | | | - Mariana Köber
- Institut
de Ciència de Materials de Barcelona (ICMAB-CSIC), Campus UAB, Bellaterra 08193, Spain
- Biomedical
Research Networking Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Madrid 28029, Spain
| | - Adriana R. Kyvik
- Institut
de Ciència de Materials de Barcelona (ICMAB-CSIC), Campus UAB, Bellaterra 08193, Spain
- Biomedical
Research Networking Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Madrid 28029, Spain
| | - Judit Tomsen-Melero
- Institut
de Ciència de Materials de Barcelona (ICMAB-CSIC), Campus UAB, Bellaterra 08193, Spain
- Biomedical
Research Networking Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Madrid 28029, Spain
| | - Guillem Vargas-Nadal
- Institut
de Ciència de Materials de Barcelona (ICMAB-CSIC), Campus UAB, Bellaterra 08193, Spain
| | - Jose Muñoz
- Institut
de Ciència de Materials de Barcelona (ICMAB-CSIC), Campus UAB, Bellaterra 08193, Spain
| | - Daniel Pulido
- Biomedical
Research Networking Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Madrid 28029, Spain
- Unidad
de Péptidos, UB, Unidad asociada
al CSIC por el IQAC, Barcelona 08028, Spain
| | - Edgar Cristóbal-Lecina
- Biomedical
Research Networking Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Madrid 28029, Spain
- Unidad
de Péptidos, UB, Unidad asociada
al CSIC por el IQAC, Barcelona 08028, Spain
| | - Solène Passemard
- Institut
de Ciència de Materials de Barcelona (ICMAB-CSIC), Campus UAB, Bellaterra 08193, Spain
| | - Miriam Royo
- Biomedical
Research Networking Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Madrid 28029, Spain
- Institut
de Química Avançada de Catalunya (IQAC−CSIC), Barcelona 08034, Spain
| | - Marta Mas-Torrent
- Institut
de Ciència de Materials de Barcelona (ICMAB-CSIC), Campus UAB, Bellaterra 08193, Spain
- Biomedical
Research Networking Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Madrid 28029, Spain
| | - Jaume Veciana
- Institut
de Ciència de Materials de Barcelona (ICMAB-CSIC), Campus UAB, Bellaterra 08193, Spain
- Biomedical
Research Networking Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Madrid 28029, Spain
| | - Marina I. Giannotti
- Biomedical
Research Networking Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Madrid 28029, Spain
- Nanoprobes
and Nanoswitches group, Institute for Bioengineering of Catalonia
(IBEC), The Barcelona Institute of Science
and Technology (BIST), Barcelona 08028, Spain
- Departament
de Ciència dels Materials i Química Física, Universitat de Barcelona, Barcelona 08028, Spain
| | - Judith Guasch
- Institut
de Ciència de Materials de Barcelona (ICMAB-CSIC), Campus UAB, Bellaterra 08193, Spain
- Biomedical
Research Networking Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Madrid 28029, Spain
- Dynamic Biomimetics
for Cancer Immunotherapy, Max Planck Partner
Group, ICMAB-CSIC, Campus UAB, Bellaterra 08193, Spain
| | - Nora Ventosa
- Institut
de Ciència de Materials de Barcelona (ICMAB-CSIC), Campus UAB, Bellaterra 08193, Spain
- Biomedical
Research Networking Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Madrid 28029, Spain
| | - Imma Ratera
- Institut
de Ciència de Materials de Barcelona (ICMAB-CSIC), Campus UAB, Bellaterra 08193, Spain
- Biomedical
Research Networking Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Madrid 28029, Spain
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24
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Kindi H, Willems C, Zhao M, Menzel M, Schmelzer CEH, Herzberg M, Fuhrmann B, Gallego-Ferrer G, Groth T. Metal Ion Doping of Alginate-Based Surface Coatings Induces Adipogenesis of Stem Cells. ACS Biomater Sci Eng 2022; 8:4327-4340. [PMID: 36174215 DOI: 10.1021/acsbiomaterials.2c00444] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/28/2022]
Abstract
Metal ions are important effectors of protein and cell functions. Here, polyelectrolyte multilayers (PEMs) made of chitosan (Chi) and alginate (Alg) were doped with different metal ions (Ca2+, Co2+, Cu2+, and Fe3+), which can form bonds with their functional groups. Ca2+ and Fe3+ ions can be deposited in PEM at higher quantities resulting in more positive ζ potentials and also higher water contact angles in the case of Fe3+. An interesting finding was that the exposure of PEM to metal ions decreases the elastic modulus of PEM. Fourier transformed infrared (FTIR) spectroscopy of multilayers provides evidence of interaction of metal ions with the carboxylic groups of Alg but not for hydroxyl and amino groups. The observed changes in wetting and surface potential are partly related to the increased adhesion and proliferation of multipotent C3H10T1/2 fibroblasts in contrast to plain nonadhesive [Chi/Alg] multilayers. Specifically, PEMs doped with Cu2+ and Fe3+ ions greatly promote cell attachment and adipogenic differentiation, which indicates that changes in not only surface properties but also the bioactivity of metal ions play an important role. In conclusion, metal ion-doped multilayer coatings made of alginate and chitosan can promote the differentiation of multipotent cells on implants without the use of other morphogens like growth factors.
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Affiliation(s)
- Husnia Kindi
- Institute of Pharmacy, Department Biomedical Materials, Martin Luther University Halle-Wittenberg, Heinrich-Damerow Strasse 4, 06120 Halle (Saale), Germany
| | - Christian Willems
- Institute of Pharmacy, Department Biomedical Materials, Martin Luther University Halle-Wittenberg, Heinrich-Damerow Strasse 4, 06120 Halle (Saale), Germany
| | - Mingyan Zhao
- Stem Cell Research and Cellular Therapy Center, Affiliated Hospital of Guangdong Medical University, Zhanjiang, 524003, China
| | - Matthias Menzel
- Department of Biological and Macromolecular Materials, Fraunhofer Institute for Microstructure of Materials and Systems IMWS, Walter-Hülse-Strasse 1, 06120 Halle (Saale), Germany
| | - Christian E H Schmelzer
- Department of Biological and Macromolecular Materials, Fraunhofer Institute for Microstructure of Materials and Systems IMWS, Walter-Hülse-Strasse 1, 06120 Halle (Saale), Germany
| | - Martin Herzberg
- Molecular Microbiology, Institute for Biology/Microbiology, Martin-Luther-University, Halle- Wittenberg, Kurt-Mothes-Strasse 3, 06120 Halle (Saale), Germany
| | - Bodo Fuhrmann
- Institute of Physics, Martin Luther University Halle-Wittenberg, Heinrich-Damerow-Strasse 4, 06120 Halle (Saale), Germany.,Interdisciplinary Center of Materials Science, Martin Luther University Halle-Wittenberg, Heinrich-Damerow-Strasse 4, 06120 Halle (Saale), Germany
| | - Gloria Gallego-Ferrer
- Centre for Biomaterials and Tissue Engineering, Universitat Politècnica de València, Camino de Vera s/n, 46022 Valencia, Spain.,Biomedical Research Networking Centre in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), 46022 Valencia, Spain
| | - Thomas Groth
- Institute of Pharmacy, Department Biomedical Materials, Martin Luther University Halle-Wittenberg, Heinrich-Damerow Strasse 4, 06120 Halle (Saale), Germany.,Interdisciplinary Center of Materials Science, Martin Luther University Halle-Wittenberg, Heinrich-Damerow-Strasse 4, 06120 Halle (Saale), Germany
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25
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Muneyasu R, Yamada T, Akai-Kasaya M, Kato HS. Self-assembly of heterogeneous bilayers stratified by Au-S and hydrogen bonds on Au(111). Phys Chem Chem Phys 2022; 24:22222-22230. [PMID: 36097862 DOI: 10.1039/d2cp03356a] [Citation(s) in RCA: 3] [Impact Index Per Article: 1.0] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/21/2022]
Abstract
The self-assembly of heterogeneous bilayers on Au substrates was investigated using atomic force microscopy (AFM), X-ray photoelectron spectroscopy (XPS), and infrared reflection absorption spectroscopy (IRAS). The formation of a well-defined bilayer including different types of functional groups would be one of the desired goals to create varying surface functionalities. In this study, we examined the assembly of a hydrogen-bonded molecular layer to another functional alkanethiolate self-assembled monolayer (SAM) on the Au(111) surface. The chemical properties and bond strength of the hydrogen bonds at the interlayer differ from those of the Au-S bonds at the anchor of thiolate SAMs, therefore the adsorbed molecules are expected to form a stratified bilayer. In this study, on one hand, we revealed that imidazole-terminated alkanethiolate SAMs (Im-SAMs) have an atomically smooth topography but chemically inhomogeneous Au-S anchors, rather incomplete than n-alkanethiolate SAMs, on the Au(111) surface. On the other hand, we confirmed the self-assembly of the heterogeneous bilayers including Im-SAMs on the Au(111) surface, even in a mixed solution containing two types of molecules. These results show that the self-assembly of the bilayer stratified by H bonds and Au-S bonds is flexible and adaptable.
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Affiliation(s)
- Riku Muneyasu
- Department of Chemistry, Graduate School of Science, Osaka University, Toyonaka, Osaka 560-0043, Japan
| | - Takashi Yamada
- Department of Chemistry, Graduate School of Science, Osaka University, Toyonaka, Osaka 560-0043, Japan
| | - Megumi Akai-Kasaya
- Department of Chemistry, Graduate School of Science, Osaka University, Toyonaka, Osaka 560-0043, Japan
| | - Hiroyuki S Kato
- Department of Chemistry, Graduate School of Science, Osaka University, Toyonaka, Osaka 560-0043, Japan
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26
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Wang R, Ni S, Ma L, Li M. Porous construction and surface modification of titanium-based materials for osteogenesis: A review. Front Bioeng Biotechnol 2022; 10:973297. [PMID: 36091459 PMCID: PMC9452912 DOI: 10.3389/fbioe.2022.973297] [Citation(s) in RCA: 18] [Impact Index Per Article: 6.0] [Reference Citation Analysis] [Abstract] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 06/20/2022] [Accepted: 07/29/2022] [Indexed: 11/13/2022] Open
Abstract
Titanium and titanium alloy implants are essential for bone tissue regeneration engineering. The current trend is toward the manufacture of implants from materials that mimic the structure, composition and elasticity of bones. Titanium and titanium alloy implants, the most common materials for implants, can be used as a bone conduction material but cannot promote osteogenesis. In clinical practice, there is a high demand for implant surfaces that stimulate bone formation and accelerate bone binding, thus shortening the implantation-to-loading time and enhancing implantation success. To avoid stress shielding, the elastic modulus of porous titanium and titanium alloy implants must match that of bone. Micro-arc oxidation technology has been utilized to increase the surface activity and build a somewhat hard coating on porous titanium and titanium alloy implants. More recently, a growing number of researchers have combined micro-arc oxidation with hydrothermal, ultrasonic, and laser treatments, coatings that inhibit bacterial growth, and acid etching with sand blasting methods to improve bonding to bone. This paper summarizes the reaction at the interface between bone and implant material, the porous design principle of scaffold material, MAO technology and the combination of MAO with other technologies in the field of porous titanium and titanium alloys to encourage their application in the development of medical implants.
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Affiliation(s)
- Rui Wang
- Department of Stomatology, The Second Hospital of Jilin University, Changchun, China
| | - Shilei Ni
- Department of Plastic and Aesthetic Surgery, Hospital of Stomatology, Jilin University, Changchun, China
| | - Li Ma
- Department of Fever Clinic, The Second Hospital of Jilin University, Changchun, China
| | - Meihua Li
- Department of Stomatology, The Second Hospital of Jilin University, Changchun, China
- *Correspondence: Meihua Li,
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27
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Lu YT, Zeng K, Fuhrmann B, Woelk C, Zhang K, Groth T. Engineering of Stable Cross-Linked Multilayers Based on Thermo-Responsive PNIPAM- Grafted-Chitosan/Heparin to Tailor Their Physiochemical Properties and Biocompatibility. ACS APPLIED MATERIALS & INTERFACES 2022; 14:29550-29562. [PMID: 35737877 DOI: 10.1021/acsami.2c05297] [Citation(s) in RCA: 8] [Impact Index Per Article: 2.7] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 06/15/2023]
Abstract
The thermo-responsive poly(N-isopropylacrylamide) (PNIPAM) is ubiquitously applied in controlled drug release and tissue engineering. However, the lack of bioactivity of PNIPAM restricts its use in cell-containing systems being a thermo-responsive adhesive substratum with no regulating effect on cell growth and differentiation. In this study, integrating PNIPAM with chitosan into PNIPAM-grafted-chitosan (PNIPAM-Chi) allows a layer-by-layer assembly with bioactive heparin to fabricate PNIPAM-modified polyelectrolyte multilayers (PNIPAM-PEMs). Grafting PNIPAM chains of either 2 (LMW) or 10 kDa (HMW) on the chitosan backbone influences the cloud point (CP) temperature in the range from 31 to 33 °C. PNIPAM-Chi with either a higher molecular weight or a higher degree of substitution of PNIPAM chains exhibiting a significant increase in diameter above CP as ensured by dynamic light scattering is selected to fabricate PEM with heparin as a polyanion at pH 4. Little difference of layer growth is detected between the chosen PNIPAM-Chi used as polycations by surface plasmon resonance, while multilayers formed with HMW-0.02 are more hydrated and show striking swelling-and-shrinking abilities when studied with quartz crystal microbalance with dissipation monitoring. Subsequently, the multilayers are covalently cross-linked using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide/N-hydroxysuccinimide to strengthen the stability of the systems under physiological conditions. Ellipsometry results confirm the layer integrity after exposure to the physiological buffer at pH 7.4 compared to those without cross-linking. Moreover, significantly higher adhesion and more spreading of C3H10T1/2 multipotent embryonic mouse fibroblasts on cross-linked PEMs, particularly with heparin terminal layers, are observed owing to the bioactivity of heparin. The slightly more hydrophobic surfaces of cross-linked PNIPAM-PEMs at 37 °C also increase cell attachment and growth. Thus, layer-by-layer constructed PNIPAM-PEM with cross-linking represents an interesting cell culture system that can be potentially employed for thermally uploading and controlled release of growth factors that further promotes tissue regeneration.
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Affiliation(s)
- Yi-Tung Lu
- Department of Biomedical Materials, Institute of Pharmacy, Martin Luther University Halle-Wittenberg, Heinrich-Damerow-Strasse 4, 06120 Halle (Saale), Germany
| | - Kui Zeng
- Department of Wood Technology and Wood-based Composites, Georg-August-University of Göttingen, Büsgenweg 4, 37077 Göttingen, Germany
| | - Bodo Fuhrmann
- Interdisciplinary Center of Material Science, Martin Luther University Halle-Wittenberg, 06099 Halle (Saale), Germany
| | - Christian Woelk
- Pharmaceutical Technology, Institute of Pharmacy, Faculty of Medicine, Leipzig University, 04317 Leipzig, Germany
| | - Kai Zhang
- Department of Wood Technology and Wood-based Composites, Georg-August-University of Göttingen, Büsgenweg 4, 37077 Göttingen, Germany
| | - Thomas Groth
- Department of Biomedical Materials, Institute of Pharmacy, Martin Luther University Halle-Wittenberg, Heinrich-Damerow-Strasse 4, 06120 Halle (Saale), Germany
- Interdisciplinary Center of Material Science, Martin Luther University Halle-Wittenberg, 06099 Halle (Saale), Germany
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28
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Electrospun Scaffolds as Cell Culture Substrates for the Cultivation of an In Vitro Blood-Brain Barrier Model Using Human Induced Pluripotent Stem Cells. Pharmaceutics 2022; 14:pharmaceutics14061308. [PMID: 35745880 PMCID: PMC9231001 DOI: 10.3390/pharmaceutics14061308] [Citation(s) in RCA: 12] [Impact Index Per Article: 4.0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 05/31/2022] [Revised: 06/16/2022] [Accepted: 06/16/2022] [Indexed: 11/17/2022] Open
Abstract
The human blood–brain barrier (BBB) represents the interface of microvasculature and the central nervous system, regulating the transport of nutrients and protecting the brain from external threats. To gain a deeper understanding of (patho)physiological processes affecting the BBB, sophisticated models mimicking the in vivo situation are required. Currently, most in vitro models are cultivated on stiff, semipermeable, and non-biodegradable Transwell® membrane inserts, not adequately mimicking the complexity of the extracellular environment of the native human BBB. To overcome these disadvantages, we developed three-dimensional electrospun scaffolds resembling the natural structure of the human extracellular matrix. The polymer fibers of the scaffold imitate collagen fibrils of the human basement membrane, exhibiting excellent wettability and biomechanical properties, thus facilitating cell adhesion, proliferation, and migration. Cultivation of human induced pluripotent stem cells (hiPSCs) on these scaffolds enabled the development of a physiological BBB phenotype monitored via the formation of tight junctions and validated by the paracellular permeability of sodium fluorescein, further accentuating the non-linearity of TEER and barrier permeability. The novel in vitro model of the BBB forms a tight endothelial barrier, offering a platform to study barrier functions in a (patho)physiologically relevant context.
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29
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Zhou J, Nie Y, Jin C, Zhang JXJ. Engineering Biomimetic Extracellular Matrix with Silica Nanofibers: From 1D Material to 3D Network. ACS Biomater Sci Eng 2022; 8:2258-2280. [PMID: 35377596 DOI: 10.1021/acsbiomaterials.1c01525] [Citation(s) in RCA: 3] [Impact Index Per Article: 1.0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 12/19/2022]
Abstract
Biomaterials at nanoscale is a fast-expanding research field with which extensive studies have been conducted on understanding the interactions between cells and their surrounding microenvironments as well as intracellular communications. Among many kinds of nanoscale biomaterials, mesoporous fibrous structures are especially attractive as a promising approach to mimic the natural extracellular matrix (ECM) for cell and tissue research. Silica is a well-studied biocompatible, natural inorganic material that can be synthesized as morpho-genetically active scaffolds by various methods. This review compares silica nanofibers (SNFs) to other ECM materials such as hydrogel, polymers, and decellularized natural ECM, summarizes fabrication techniques for SNFs, and discusses different strategies of constructing ECM using SNFs. In addition, the latest progress on SNFs synthesis and biomimetic ECM substrates fabrication is summarized and highlighted. Lastly, we look at the wide use of SNF-based ECM scaffolds in biological applications, including stem cell regulation, tissue engineering, drug release, and environmental applications.
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Affiliation(s)
- Junhu Zhou
- Thayer School of Engineering, Dartmouth College, Hanover, New Hampshire 03755, United States
| | - Yuan Nie
- Thayer School of Engineering, Dartmouth College, Hanover, New Hampshire 03755, United States
| | - Congran Jin
- Thayer School of Engineering, Dartmouth College, Hanover, New Hampshire 03755, United States
| | - John X J Zhang
- Thayer School of Engineering, Dartmouth College, Hanover, New Hampshire 03755, United States
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30
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Islam MM, Chivu A, AbuSamra DB, Saha A, Chowdhuri S, Pramanik B, Dohlman CH, Das D, Argüeso P, Rajaiya J, Patra HK, Chodosh J. Crosslinker-free collagen gelation for corneal regeneration. Sci Rep 2022; 12:9108. [PMID: 35650270 PMCID: PMC9160259 DOI: 10.1038/s41598-022-13146-9] [Citation(s) in RCA: 12] [Impact Index Per Article: 4.0] [Reference Citation Analysis] [Abstract] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 03/17/2022] [Accepted: 05/20/2022] [Indexed: 12/13/2022] Open
Abstract
Development of an artificial cornea can potentially fulfil the demand of donor corneas for transplantation as the number of donors is far less than needed to treat corneal blindness. Collagen-based artificial corneas stand out as a regenerative option, having promising clinical outcomes. Collagen crosslinked with chemical crosslinkers which modify the parent functional groups of collagen. However, crosslinkers are usually cytotoxic, so crosslinkers need to be removed from implants completely before application in humans. In addition, crosslinked products are mechanically weak and susceptible to enzymatic degradation. We developed a crosslinker free supramolecular gelation strategy using pyrene conjugated dipeptide amphiphile (PyKC) consisting of lysine and cysteine; in which collagen molecules are intertwined inside the PyKC network without any functional group modification of the collagen. The newly developed collagen implants (Coll-PyKC) are optically transparent and can effectively block UV light, are mechanically and enzymatically stable, and can be sutured. The Coll-PyKC implants support the growth and function of all corneal cells, trigger anti-inflammatory differentiation while suppressing the pro-inflammatory differentiation of human monocytes. Coll-PyKC implants can restrict human adenovirus propagation. Therefore, this crosslinker-free strategy can be used for the repair, healing, and regeneration of the cornea, and potentially other damaged organs of the body.
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Affiliation(s)
- Mohammad Mirazul Islam
- Department of Ophthalmology, Massachusetts Eye and Ear and Schepens Eye Research Institute, Harvard Medical School, Boston, MA, 02114, USA
| | - Alexandru Chivu
- Department of Surgical Biotechnology, University College London, London, NW3 2PF, UK
| | - Dina B AbuSamra
- Department of Ophthalmology, Massachusetts Eye and Ear and Schepens Eye Research Institute, Harvard Medical School, Boston, MA, 02114, USA
| | - Amrita Saha
- Department of Ophthalmology, Massachusetts Eye and Ear and Schepens Eye Research Institute, Harvard Medical School, Boston, MA, 02114, USA
| | - Sumit Chowdhuri
- Department of Chemistry, Indian Institute of Technology Guwahati, Guwahati, Assam, 781039, India
| | - Bapan Pramanik
- Department of Chemistry, Ben Gurion University of the Negev, Be'er Sheva, Israel
| | - Claes H Dohlman
- Department of Ophthalmology, Massachusetts Eye and Ear and Schepens Eye Research Institute, Harvard Medical School, Boston, MA, 02114, USA
| | - Debapratim Das
- Department of Chemistry, Indian Institute of Technology Guwahati, Guwahati, Assam, 781039, India
| | - Pablo Argüeso
- Department of Ophthalmology, Massachusetts Eye and Ear and Schepens Eye Research Institute, Harvard Medical School, Boston, MA, 02114, USA
| | - Jaya Rajaiya
- Department of Ophthalmology, Massachusetts Eye and Ear and Schepens Eye Research Institute, Harvard Medical School, Boston, MA, 02114, USA
| | - Hirak K Patra
- Department of Surgical Biotechnology, University College London, London, NW3 2PF, UK.
| | - James Chodosh
- Department of Ophthalmology, Massachusetts Eye and Ear and Schepens Eye Research Institute, Harvard Medical School, Boston, MA, 02114, USA.
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31
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Sahebalzamani M, Ziminska M, McCarthy HO, Levingstone TJ, Dunne NJ, Hamilton AR. Advancing bone tissue engineering one layer at a time: a layer-by-layer assembly approach to 3D bone scaffold materials. Biomater Sci 2022; 10:2734-2758. [PMID: 35438692 DOI: 10.1039/d1bm01756j] [Citation(s) in RCA: 19] [Impact Index Per Article: 6.3] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 12/11/2022]
Abstract
The layer-by-layer (LbL) assembly technique has shown excellent potential in tissue engineering applications. The technique is mainly based on electrostatic attraction and involves the sequential adsorption of oppositely charged electrolyte complexes onto a substrate, resulting in uniform single layers that can be rapidly deposited to form nanolayer films. LbL has attracted significant attention as a coating technique due to it being a convenient and affordable fabrication method capable of achieving a wide range of biomaterial coatings while keeping the main biofunctionality of the substrate materials. One promising application is the use of nanolayer films fabricated by LbL assembly in the development of 3-dimensional (3D) bone scaffolds for bone repair and regeneration. Due to their versatility, nanoscale films offer an exciting opportunity for tailoring surface and bulk property modification of implants for osseous defect therapies. This review article discusses the state of the art of the LbL assembly technique, and the properties and functions of LbL-assembled films for engineered bone scaffold application, combination of multilayers for multifunctional coatings and recent advancements in the application of LbL assembly in bone tissue engineering. The recent decade has seen tremendous advances in the promising developments of LbL film systems and their impact on cell interaction and tissue repair. A deep understanding of the cell behaviour and biomaterial interaction for the further development of new generations of LbL films for tissue engineering are the most important targets for biomaterial research in the field. While there is still much to learn about the biological and physicochemical interactions at the interface of nano-surface coated scaffolds and biological systems, we provide a conceptual review to further progress in the LbL approach to 3D bone scaffold materials and inform the future of LbL development in bone tissue engineering.
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Affiliation(s)
- MohammadAli Sahebalzamani
- School of Mechanical and Manufacturing Engineering, Dublin City University, Dublin 9, Ireland. .,Centre for Medical Engineering Research, Dublin City University, Dublin 9, Ireland.
| | - Monika Ziminska
- School of Pharmacy, Queen's University Belfast, Belfast BT9 7BL, UK.
| | - Helen O McCarthy
- School of Pharmacy, Queen's University Belfast, Belfast BT9 7BL, UK. .,School of Chemical Sciences, Dublin City University, Dublin 9, Ireland
| | - Tanya J Levingstone
- School of Mechanical and Manufacturing Engineering, Dublin City University, Dublin 9, Ireland. .,Centre for Medical Engineering Research, Dublin City University, Dublin 9, Ireland. .,Advanced Manufacturing Research Centre (I-Form), School of Mechanical and Manufacturing Engineering, Dublin City University, Dublin 9, Ireland.,Trinity Centre for Biomedical Engineering, Trinity Biomedical Sciences Institute, Trinity College Dublin, Dublin 2, Ireland.,Advanced Processing Technology Research Centre, Dublin City University, Dublin 9, Ireland.,Biodesign Europe, Dublin City University, Dublin 9, Ireland
| | - Nicholas J Dunne
- School of Mechanical and Manufacturing Engineering, Dublin City University, Dublin 9, Ireland. .,Centre for Medical Engineering Research, Dublin City University, Dublin 9, Ireland. .,School of Pharmacy, Queen's University Belfast, Belfast BT9 7BL, UK. .,Department of Mechanical and Manufacturing Engineering, School of Engineering, Trinity College Dublin, Dublin 2, Ireland.,Advanced Manufacturing Research Centre (I-Form), School of Mechanical and Manufacturing Engineering, Dublin City University, Dublin 9, Ireland.,Advanced Materials and Bioengineering Research Centre (AMBER), Trinity College Dublin, Dublin 2, Ireland.,Trinity Centre for Biomedical Engineering, Trinity Biomedical Sciences Institute, Trinity College Dublin, Dublin 2, Ireland.,Advanced Processing Technology Research Centre, Dublin City University, Dublin 9, Ireland.,Biodesign Europe, Dublin City University, Dublin 9, Ireland
| | - Andrew R Hamilton
- Faculty of Engineering and Physical Sciences, University of Southampton, Southampton SO17 1BJ, UK.
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32
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Zhang Z, Li JB, Li X, Zhu CY, Ren L, Huang XJ, Wu J, Ji J, Xu ZK. Janus membranes with asymmetric cellular adhesion behaviors for regenerating eardrum perforation. J Mater Chem B 2022; 10:2719-2727. [PMID: 35138320 DOI: 10.1039/d1tb02418c] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 02/05/2023]
Abstract
The tympanic membrane plays an important role in the human hearing system, which is easily perforated under unfavorable conditions, leading to loss of hearing and otitis media. Many autologous materials and artificial materials have been used to repair a perforated tympanic membrane, but these materials sometimes can cause severe hearing loss because of their adhesion to the ossicle during the healing process and the postoperative process. Herein, we report Janus membranes with asymmetric cellular adhesion behaviors for regenerating the eardrum. These Janus membranes are constructed by co-depositing a tannic acid (TA)/3-aminopropyltriethoxysilane (APTES) coating on one surface of the polypropylene microfiltration membrane. Cellular experiments indicate that the Janus membranes have good biocompatibility and asymmetric cellular adhesion properties. The repair of the tympanic membrane perforation experiment and laser Doppler vibrometer (LDV) measurements prove that the hydrophilic surface of Janus membranes repairs perforated eardrums, and meanwhile the hydrophobic surface can avoid adhering to the inner ear tissue for reducing hearing loss. The Janus membranes have good prospects in the treatment of tympanic membrane perforation.
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Affiliation(s)
- Zhili Zhang
- Department of Otorhinolaryngology Head and Neck Surgery, The first affiliated hospital of Zhejiang University School of Medicine, Hangzhou 310003, China
| | - Jin-Bo Li
- Department of Chemistry, Zhejiang University, Hangzhou 310027, China.
| | - Xu Li
- MOE Key Laboratory of Macromolecular Synthesis and Functionalization, and Key Laboratory of Adsorption and Separation Materials & Technologies of Zhejiang Province, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China.
| | - Cheng-Ye Zhu
- MOE Key Laboratory of Macromolecular Synthesis and Functionalization, and Key Laboratory of Adsorption and Separation Materials & Technologies of Zhejiang Province, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China.
| | - Liujie Ren
- Department of FPRS, Eye & ENT Hospital of Fudan University, Shanghai 200031, China.
| | - Xiao-Jun Huang
- MOE Key Laboratory of Macromolecular Synthesis and Functionalization, and Key Laboratory of Adsorption and Separation Materials & Technologies of Zhejiang Province, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China.
| | - Jian Wu
- Department of Chemistry, Zhejiang University, Hangzhou 310027, China.
| | - Jian Ji
- MOE Key Laboratory of Macromolecular Synthesis and Functionalization, and Key Laboratory of Adsorption and Separation Materials & Technologies of Zhejiang Province, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China.
| | - Zhi-Kang Xu
- MOE Key Laboratory of Macromolecular Synthesis and Functionalization, and Key Laboratory of Adsorption and Separation Materials & Technologies of Zhejiang Province, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China.
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33
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Stengelin E, Thiele J, Seiffert S. Multiparametric Material Functionality of Microtissue-Based In Vitro Models as Alternatives to Animal Testing. ADVANCED SCIENCE (WEINHEIM, BADEN-WURTTEMBERG, GERMANY) 2022; 9:e2105319. [PMID: 35043598 PMCID: PMC8981905 DOI: 10.1002/advs.202105319] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.3] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Grants] [Track Full Text] [Subscribe] [Scholar Register] [Received: 11/30/2021] [Indexed: 05/12/2023]
Abstract
With the definition of the 3R principle by Russel and Burch in 1959, the search for an adequate substitute for animal testing has become one of the most important tasks and challenges of this time, not only from an ethical, but also from a scientific, economic, and legal point of view. Microtissue-based in vitro model systems offer a valuable approach to address this issue by accounting for the complexity of natural tissues in a simplified manner. To increase the functionality of these model systems and thus make their use as a substitute for animal testing more likely in the future, the fundamentals need to be continuously improved. Corresponding requirements exist in the development of multifunctional, hydrogel-based materials, whose properties are considered in this review under the aspects of processability, adaptivity, biocompatibility, and stability/degradability.
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Affiliation(s)
- Elena Stengelin
- Department of ChemistryJohannes Gutenberg‐University MainzD‐55128MainzGermany
| | - Julian Thiele
- Leibniz‐Institut für Polymerforschung Dresden e.V.Hohe Straße 6D‐01069DresdenGermany
| | - Sebastian Seiffert
- Department of ChemistryJohannes Gutenberg‐University MainzD‐55128MainzGermany
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34
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Bandzerewicz A, Gadomska-Gajadhur A. Into the Tissues: Extracellular Matrix and Its Artificial Substitutes: Cell Signalling Mechanisms. Cells 2022; 11:914. [PMID: 35269536 PMCID: PMC8909573 DOI: 10.3390/cells11050914] [Citation(s) in RCA: 50] [Impact Index Per Article: 16.7] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 02/17/2022] [Revised: 03/02/2022] [Accepted: 03/04/2022] [Indexed: 02/06/2023] Open
Abstract
The existence of orderly structures, such as tissues and organs is made possible by cell adhesion, i.e., the process by which cells attach to neighbouring cells and a supporting substance in the form of the extracellular matrix. The extracellular matrix is a three-dimensional structure composed of collagens, elastin, and various proteoglycans and glycoproteins. It is a storehouse for multiple signalling factors. Cells are informed of their correct connection to the matrix via receptors. Tissue disruption often prevents the natural reconstitution of the matrix. The use of appropriate implants is then required. This review is a compilation of crucial information on the structural and functional features of the extracellular matrix and the complex mechanisms of cell-cell connectivity. The possibilities of regenerating damaged tissues using an artificial matrix substitute are described, detailing the host response to the implant. An important issue is the surface properties of such an implant and the possibilities of their modification.
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35
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Benkocká M, Herma R, Lupínková S, Slepička P, Švorčík V, Kolská Z. Antibacterial nanocomposite supporting cell growth and spheroid formation by chemical surface treatment of polymer foil. SURF INTERFACE ANAL 2022. [DOI: 10.1002/sia.7082] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/10/2022]
Affiliation(s)
- M. Benkocká
- Faculty of Science, J. E. Purkinje University in Usti nad Labem Usti nad Labem Czech Republic
| | - R. Herma
- Faculty of Science, J. E. Purkinje University in Usti nad Labem Usti nad Labem Czech Republic
| | - S. Lupínková
- Faculty of Science, J. E. Purkinje University in Usti nad Labem Usti nad Labem Czech Republic
| | - P. Slepička
- Institute of Solid State Engineering, University of Chemistry and Technology Prague Prague 6 Czech Republic
| | - V. Švorčík
- Institute of Solid State Engineering, University of Chemistry and Technology Prague Prague 6 Czech Republic
| | - Z. Kolská
- Faculty of Science, J. E. Purkinje University in Usti nad Labem Usti nad Labem Czech Republic
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36
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Ossowska A, Ryl J, Sternicki T. Production and Properties of the Porous Layer Obtained by the Electrochemical Method on the Surface of Austenitic Steel. MATERIALS 2022; 15:ma15030949. [PMID: 35160903 PMCID: PMC8837965 DOI: 10.3390/ma15030949] [Citation(s) in RCA: 3] [Impact Index Per Article: 1.0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Subscribe] [Scholar Register] [Received: 11/30/2021] [Revised: 01/16/2022] [Accepted: 01/19/2022] [Indexed: 12/10/2022]
Abstract
The growing demand for implants has seen increasing interest in the introduction of new technologies and surface modification methods of metal biomaterials. This research aimed to produce and characterize a porous layer grown on austenitic stainless steel 316L, obtained via the anodization process near the micro-arc oxidation, i.e., low voltage micro-arc oxidation (LVMAO). The discussed layer significantly influences the properties of metallic biomedical materials. The surface topography, layer thickness, surface roughness, pore diameter, elemental composition, crystal structure, and surface wettability were assessed for all anodized layers, together with the resultant corrosion resistance. Attention was paid to the influence of the process parameters that affect the specification of the produced layer. The obtained results showed surface development and different sized pores in the modified layers, as well as an increase in corrosion resistance in the Ringer’s solution.
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Affiliation(s)
- Agnieszka Ossowska
- Faculty of Mechanical Engineering and Ship Technology, Gdansk University of Technology, 80-233 Gdańsk, Poland;
- Correspondence:
| | - Jacek Ryl
- Advanced Materials Center, Faculty of Applied Physics and Mathematics, Gdansk University of Technology, 80-233 Gdańsk, Poland;
| | - Tomasz Sternicki
- Faculty of Mechanical Engineering and Ship Technology, Gdansk University of Technology, 80-233 Gdańsk, Poland;
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37
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Tewari M, Pareek P, Kumar S. Correlating Amino Acid Interaction with Graphene-Based Materials Regulating Cell Function. J Indian Inst Sci 2022. [DOI: 10.1007/s41745-021-00272-y] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.3] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 01/05/2023]
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38
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Enkhbat M, Zhong B, Chang R, Geng J, Lu LS, Chen YJ, Wang PY. Harnessing Focal Adhesions to Accelerate p53 Accumulation and Anoikis of A549 Cells Using Colloidal Self-Assembled Patterns (cSAPs). ACS APPLIED BIO MATERIALS 2022; 5:322-333. [PMID: 35034455 DOI: 10.1021/acsabm.1c01109] [Citation(s) in RCA: 6] [Impact Index Per Article: 2.0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 01/16/2023]
Abstract
Extracellular matrix (ECM) of the tumor microenvironment (TME), including topography and biological molecules, is crucial in cancer cell attachment, growth, and even the sensitivity to the chemo and cell drugs treatment. This study hypothesizes that mimic ECM structures can alter the attachment and drug sensitivity of cancer cells. A family of artificial ECM called colloidal self-assembled patterns (cSAPs) was fabricated to mimic tumor ECM structures. Cell adhesion, proliferation, and drug sensitivity of the A549 non-small cell lung cancer (NSCLC) cells were studied on 24 cSAPs, named cSAP#1-cSAP#24, where surface topography and wettability were distinct. The results showed that cell adhesion and cell spreading were generally reduced on cSAPs compared to the flat controls. In addition, the synergistic effect of cSAPs and several chemo drugs on cell survival was investigated. Interestingly, A549 cells were more sensitive to the combination of doxorubicin and cSAP#4. Under this condition, the focal adhesion kinase (FAK) signaling was downregulated while p53 signaling was upregulated, confirmed by real-time PCR and western blot analysis. It indicates that the specific surface structure could induce higher drug sensitivity and in vitro anoikis of A549 cells. A serum alternative, human platelet lysate (hPL), and different cSAPs were examined to verify our hypothesis. The result further confirmed that cell adhesion strongly affected the drug sensitivity of A549 cells. This study demonstrates that the tumor ECM is vital in cancer cell activity and drug sensitivity; therefore, it should be considered in drug discovery and therapeutic regimens.
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Affiliation(s)
- Myagmartsend Enkhbat
- Oujiang Laboratory, Wenzhou, Zhejiang 325000, China.,Shenzhen Key Laboratory of Biomimetic Materials and Cellular Immunomodulation, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, Guangdong 518055, China
| | - Boya Zhong
- Shenzhen Key Laboratory of Biomimetic Materials and Cellular Immunomodulation, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, Guangdong 518055, China
| | - Ray Chang
- Shenzhen Key Laboratory of Biomimetic Materials and Cellular Immunomodulation, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, Guangdong 518055, China
| | - Jin Geng
- Shenzhen Key Laboratory of Biomimetic Materials and Cellular Immunomodulation, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, Guangdong 518055, China
| | - Long-Sheng Lu
- Graduate Institute of Biomedical Materials and Tissue Engineering, College of Biomedical Engineering, Taipei Medical University, Taipei 110, Taiwan
| | - Yin-Ju Chen
- Graduate Institute of Biomedical Materials and Tissue Engineering, College of Biomedical Engineering, Taipei Medical University, Taipei 110, Taiwan
| | - Peng-Yuan Wang
- Oujiang Laboratory, Wenzhou, Zhejiang 325000, China.,Shenzhen Key Laboratory of Biomimetic Materials and Cellular Immunomodulation, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, Guangdong 518055, China
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39
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Bělinová T, Javorová P, Nguyenová HY, Řezníčková A, Humlová Z, Hubálek Kalbáčová M. Ultra-Small Gold Nanoparticles with Mild Immunomodulatory Activity as a Potential Tool for Bio-Applications. Folia Biol (Praha) 2022; 68:142-152. [PMID: 36871170 DOI: 10.14712/fb2022068040142] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.3] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 12/17/2024]
Abstract
Recently, more and more efforts are directed towards developing new imaging and drug-delivery options based on various nanoparticles, exploiting their unique properties. Here, ultra-small gold nanoparticles functionalized with widely used polyethylene glycol and its amine-terminated form were tested in respect of their potential interactions with human immune cells (cell line and primary cells). The results showed that differently terminated ultrasmall gold nanoparticles represent an interesting theranostic platform as they are harmless to immune cells (not inducing cytotoxicity and severe immune response) and on the other hand, they can serve as imaging and/or drug delivery agents using e.g. monocytes/ macrophages as "Trojan horses" to deliver these nanoparticles across the blood-brain barrier and diagnose or treat pathologies of the central nervous system.
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Affiliation(s)
- T Bělinová
- Biomedical Centre, Faculty of Medicine in Pilsen, Charles University, Czech Republic
| | - P Javorová
- Institute of Pathological Physiology, First Faculty of Medicine, Charles University, Czech Republic
| | - H Y Nguyenová
- Department of Solid State Engineering, University of Chemistry and Technology Prague, Czech Republic
| | - A Řezníčková
- Department of Solid State Engineering, University of Chemistry and Technology Prague, Czech Republic
| | - Z Humlová
- Institute of Pathological Physiology, First Faculty of Medicine, Charles University, Czech Republic
- Institute of Immunology and Microbiology, First Faculty of Medicine, Charles University and General University Hospital in Prague, Czech Republic
| | - M Hubálek Kalbáčová
- Institute of Pathological Physiology, First Faculty of Medicine, Charles University, Czech Republic
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40
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Sánchez-Bodón J, Andrade del Olmo J, Alonso JM, Moreno-Benítez I, Vilas-Vilela JL, Pérez-Álvarez L. Bioactive Coatings on Titanium: A Review on Hydroxylation, Self-Assembled Monolayers (SAMs) and Surface Modification Strategies. Polymers (Basel) 2021; 14:165. [PMID: 35012187 PMCID: PMC8747097 DOI: 10.3390/polym14010165] [Citation(s) in RCA: 29] [Impact Index Per Article: 7.3] [Reference Citation Analysis] [Abstract] [Key Words] [Grants] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 12/03/2021] [Revised: 12/28/2021] [Accepted: 12/29/2021] [Indexed: 12/15/2022] Open
Abstract
Titanium (Ti) and its alloys have been demonstrated over the last decades to play an important role as inert materials in the field of orthopedic and dental implants. Nevertheless, with the widespread use of Ti, implant-associated rejection issues have arisen. To overcome these problems, antibacterial properties, fast and adequate osseointegration and long-term stability are essential features. Indeed, surface modification is currently presented as a versatile strategy for developing Ti coatings with all these challenging requirements and achieve a successful performance of the implant. Numerous approaches have been investigated to obtain stable and well-organized Ti coatings that promote the tailoring of surface chemical functionalization regardless of the geometry and shape of the implant. However, among all the approaches available in the literature to functionalize the Ti surface, a promising strategy is the combination of surface pre-activation treatments typically followed by the development of intermediate anchoring layers (self-assembled monolayers, SAMs) that serve as the supporting linkage of a final active layer. Therefore, this paper aims to review the latest approaches in the biomedical area to obtain bioactive coatings onto Ti surfaces with a special focus on (i) the most employed methods for Ti surface hydroxylation, (ii) SAMs-mediated active coatings development, and (iii) the latest advances in active agent immobilization and polymeric coatings for controlled release on Ti surfaces.
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Affiliation(s)
- Julia Sánchez-Bodón
- Grupo de Química Macromolecular (LABQUIMAC), Departamento de Química Física, Facultad de Ciencia y Tecnología, Universidad del País Vasco UPV/EHU, 48940 Leioa, Spain; (J.S.-B.); (J.A.d.O.); (I.M.-B.); (J.L.V.-V.)
| | - Jon Andrade del Olmo
- Grupo de Química Macromolecular (LABQUIMAC), Departamento de Química Física, Facultad de Ciencia y Tecnología, Universidad del País Vasco UPV/EHU, 48940 Leioa, Spain; (J.S.-B.); (J.A.d.O.); (I.M.-B.); (J.L.V.-V.)
- i+Med S. Coop, Parque Tecnológico de Alava, Albert Einstein 15, Nave 15, 01510 Vitoria-Gasteiz, Spain;
| | - Jose María Alonso
- i+Med S. Coop, Parque Tecnológico de Alava, Albert Einstein 15, Nave 15, 01510 Vitoria-Gasteiz, Spain;
| | - Isabel Moreno-Benítez
- Grupo de Química Macromolecular (LABQUIMAC), Departamento de Química Física, Facultad de Ciencia y Tecnología, Universidad del País Vasco UPV/EHU, 48940 Leioa, Spain; (J.S.-B.); (J.A.d.O.); (I.M.-B.); (J.L.V.-V.)
| | - José Luis Vilas-Vilela
- Grupo de Química Macromolecular (LABQUIMAC), Departamento de Química Física, Facultad de Ciencia y Tecnología, Universidad del País Vasco UPV/EHU, 48940 Leioa, Spain; (J.S.-B.); (J.A.d.O.); (I.M.-B.); (J.L.V.-V.)
- BCMaterials, Basque Center for Materials, Applications and Nanostructures, UPV/EHU Science Park, 48940 Leioa, Spain
| | - Leyre Pérez-Álvarez
- Grupo de Química Macromolecular (LABQUIMAC), Departamento de Química Física, Facultad de Ciencia y Tecnología, Universidad del País Vasco UPV/EHU, 48940 Leioa, Spain; (J.S.-B.); (J.A.d.O.); (I.M.-B.); (J.L.V.-V.)
- BCMaterials, Basque Center for Materials, Applications and Nanostructures, UPV/EHU Science Park, 48940 Leioa, Spain
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41
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Zhang Z, Li Y, Zhang T, Yang X, Fan K, Wang D, Li S, Hu Y, Fu W. Titanium implants modified by laser microtexturing enhance the bioactivity of gastric epithelial cells and fibroblast cells. J Appl Biomater Funct Mater 2021; 19:22808000211064951. [PMID: 34905988 DOI: 10.1177/22808000211064951] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/16/2022] Open
Abstract
The clinical application of anastomotic instruments improves the efficiency of the digestive tract surgery. However, the stapler with titanium nails implanted is still controversial in terms of anastomotic complications, and further improvement and optimization are needed. The purpose of this study was to explore the optimal microtextured parameters that could enhance the bioactivity of titanium implants in vitro. Laser microtexturing technology was used to construct the groove-type microstructural surfaces with different parameters, and human gastric mucosal epithelial cells (GES-1 cells) and mouse fibroblasts (3T3 cells) were cultured on the surface of the titanium plates in vitro. The data of cell adhesion, cell proliferation and cell activity were obtained and statistically analyzed. The textured titanium plates meet the expected design. GES-1 and 3T3 cell adhesion were better in the surface of titanium plates in microstructural group than that in the polished group. GES-1 and 3T3 cells also showed higher proliferative activity in the microstructural group compared with the polished group. The laser textured titanium plates have good groove-type microstructure, which increase the surface roughness, change the surface wettability, promote the adhesion, proliferating and orderly growth of GES-1 and 3T3 cells, and show good biological properties.
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Affiliation(s)
- Zhaoxiong Zhang
- Department of General Surgery, Tianjin Medical University General Hospital, Tianjin, People's Republic of China
| | - Yuanchun Li
- Department of General Surgery, Tianjin Medical University General Hospital, Tianjin, People's Republic of China
| | - Ting Zhang
- Department of General Surgery, Tianjin Medical University General Hospital, Tianjin, People's Republic of China
| | - Xiaoyang Yang
- Tianjin Key Laboratory for Advanced Mechatronic System Design and Intelligent Control, School of Mechanical Engineering, National Demonstration Center for Experimental Mechanical and Electrical Engineering Education (Tianjin University of Technology), Tianjin, People's Republic of China
| | - Kaihu Fan
- Department of General Surgery, Tianjin Medical University General Hospital, Tianjin, People's Republic of China
| | - Daohan Wang
- Department of General Surgery, Tianjin Medical University General Hospital, Tianjin, People's Republic of China
| | - Shuliang Li
- Department of General Surgery, Tianjin Medical University General Hospital, Tianjin, People's Republic of China.,Department of Gastrointestinal Surgery, The Second People's Hospital of Liaocheng, Linqing, Shandong, People's Republic of China.,Department of Gastrointestinal Surgery, The Second Hospital of Liaocheng Affiliated to Shandong First Medical University, Linqing, Shandong, People's Republic of China
| | - Yahui Hu
- Tianjin Key Laboratory for Advanced Mechatronic System Design and Intelligent Control, School of Mechanical Engineering, National Demonstration Center for Experimental Mechanical and Electrical Engineering Education (Tianjin University of Technology), Tianjin, People's Republic of China
| | - Weihua Fu
- Department of General Surgery, Tianjin Medical University General Hospital, Tianjin, People's Republic of China
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42
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Brito Barrera YA, Husteden C, Alherz J, Fuhrmann B, Wölk C, Groth T. Extracellular matrix-inspired surface coatings functionalized with dexamethasone-loaded liposomes to induce osteo- and chondrogenic differentiation of multipotent stem cells. MATERIALS SCIENCE & ENGINEERING. C, MATERIALS FOR BIOLOGICAL APPLICATIONS 2021; 131:112516. [PMID: 34857295 DOI: 10.1016/j.msec.2021.112516] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Subscribe] [Scholar Register] [Received: 08/27/2021] [Revised: 10/19/2021] [Accepted: 10/22/2021] [Indexed: 10/20/2022]
Abstract
Biomimetic surface coatings can be combined with conventional implants to mimic the extracellular matrix (ECM) of the surrounding tissue to make them more biocompatible. Layer-by-layer technique (LbL) can be used for making surface coatings by alternating adsorption of polyanions and polycations from aqueous solutions without need of chemical reactions. Here, polyelectrolyte multilayer (PEM) systems is made of hyaluronic acid (HA) as polyanion and Collagen I (Col) as polycation to mimic the ECM of connective tissue. The PEM are combined with dexamethasone (Dex)-loaded liposomes to achieve a local delivery and protection of this drug for stimulation of osteo- and chondrogenic differentiation of multipotent stem cells. The liposomes possess a positive surface charge that is required for immobilization on the PEM. The surface properties of PEM system show a positive zeta potential after liposome adsorption and a decrease in wettability, both promoting cell adhesion and spreading of C3H10T1/2 multipotent embryonic mouse fibroblasts. Differentiation of C3H10T1/2 was more prominent on the PEM system with embedded Dex-loaded liposomes compared to the basal PEM system and the use of free Dex-loaded liposomes in the supernatant. This was evident by immunohistochemical staining and an upregulation of the expression of genes, which play a key role in osteogenesis (RunX2, ALP, Osteocalcin (OCN)) and chondrogenesis (Sox9, aggrecan (ACAN), collagen type II), determined by quantitative Real-time polymerase chain reaction (qRT-PCR) after 21 days. These findings indicate that the designed liposome-loaded PEM system have high potential for use as drug delivery systems for implant coatings that can induce bone and cartilage differentiation needed for example in osteochondral implants.
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Affiliation(s)
- Yazmin A Brito Barrera
- Department Biomedical Materials, Institute of Pharmacy, Martin Luther University Halle-Wittenberg, Heinrich-Damerow-Strasse 4, 06120 Halle (Saale), Germany
| | - Catharina Husteden
- Medicinal Chemistry Department, Institute of Pharmacy, Martin Luther University Halle-Wittenberg, 06120 Halle (Saale), Germany
| | - Jumanah Alherz
- Department Biomedical Materials, Institute of Pharmacy, Martin Luther University Halle-Wittenberg, Heinrich-Damerow-Strasse 4, 06120 Halle (Saale), Germany
| | - Bodo Fuhrmann
- Interdisciplinary Center of Materials Science, Martin Luther University Halle-Wittenberg, D-06099 Halle (Saale), Germany
| | - Christian Wölk
- Pharmaceutical Technology, Institute of Pharmacy, Faculty of Medicine, Leipzig University, 04317 Leipzig, Germany
| | - Thomas Groth
- Department Biomedical Materials, Institute of Pharmacy, Martin Luther University Halle-Wittenberg, Heinrich-Damerow-Strasse 4, 06120 Halle (Saale), Germany; Interdisciplinary Center of Materials Science, Martin Luther University Halle-Wittenberg, D-06099 Halle (Saale), Germany; Laboratory of Biomedical Nanotechnologies, Institute of Bionic Technologies and Engineering, I.M. Sechenov First Moscow State University, 119991, Trubetskaya street 8, Moscow, Russian Federation.
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43
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Kindi H, Menzel M, Heilmann A, Schmelzer CEH, Herzberg M, Fuhrmann B, Gallego-Ferrer G, Groth T. Effect of metal ions on the physical properties of multilayers from hyaluronan and chitosan, and the adhesion, growth and adipogenic differentiation of multipotent mouse fibroblasts. SOFT MATTER 2021; 17:8394-8410. [PMID: 34550141 DOI: 10.1039/d1sm00405k] [Citation(s) in RCA: 2] [Impact Index Per Article: 0.5] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 06/13/2023]
Abstract
Polyelectrolyte multilayers (PEMs) consisting of the polysaccharides hyaluronic acid (HA) as the polyanion and chitosan (Chi) as the polycation were prepared with layer-by-layer technique (LbL). The [Chi/HA]5 multilayers were exposed to solutions of metal ions (Ca2+, Co2+, Cu2+ and Fe3+). Binding of metal ions to [Chi/HA]5 multilayers by the formation of complexes with functional groups of polysaccharides modulates their physical properties and the bioactivity of PEMs with regard to the adhesion and function of multipotent murine C3H10T1/2 embryonic fibroblasts. Characterization of multilayer formation and surface properties using different analytical methods demonstrates changes in the wetting, surface potential and mechanical properties of multilayers depending on the concentration and type of metal ion. Most interestingly, it is observed that Fe3+ metal ions greatly promote adhesion and spreading of C3H10T1/2 cells on the low adhesive [Chi/HA]5 PEM system. The application of intermediate concentrations of Cu2+, Ca2+ and Co2+ as well as low concentrations of Fe3+ to PEMs also results in increased cell spreading. Moreover, it can be shown that complex formation of PEMs with Cu2+ and Fe3+ ions leads to increased metabolic activity in cells after 24 h and induces cell differentiation towards adipocytes in the absence of any additional adipogenic media supplements. Overall, complex formation of [Chi/HA]5 PEM with metal ions like Cu2+ and Fe3+ represents an interesting and cheap alternative to the use of growth factors for making cell-adhesive coatings and guiding stem cell differentiation on implants and scaffolds to regenerate connective-type of tissues.
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Affiliation(s)
- Husnia Kindi
- Department Biomedical Materials, Institute of Pharmacy, Martin Luther University Halle-Wittenberg, 06120 Halle (Saale), Germany.
| | - Matthias Menzel
- Department of Biological and Macromolecular Materials, Fraunhofer Institute for Microstructure of Materials and Systems IMWS, 06120 Halle (Saale), Germany
| | - Andreas Heilmann
- Department of Biological and Macromolecular Materials, Fraunhofer Institute for Microstructure of Materials and Systems IMWS, 06120 Halle (Saale), Germany
| | - Christian E H Schmelzer
- Department of Biological and Macromolecular Materials, Fraunhofer Institute for Microstructure of Materials and Systems IMWS, 06120 Halle (Saale), Germany
| | - Martin Herzberg
- Molecular Microbiology, Institute for Biology/Microbiology, Martin-Luther-University, Halle-Wittenberg, Germany
| | - Bodo Fuhrmann
- Institute of Physics, Martin Luther University Halle-Wittenberg, 06099 Halle (Saale), Germany
- Interdisciplinary Center of Materials Science, Martin Luther University Halle-Wittenberg, 06120 Halle (Saale), Germany
| | - Gloria Gallego-Ferrer
- Centre for Biomaterials and Tissue Engineering, Universitat Politècnica de València, Caminode Veras/n, 46022 Valencia, Spain
- Biomedical Research Networking Centre in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), 46022 Valencia, Spain
| | - Thomas Groth
- Department Biomedical Materials, Institute of Pharmacy, Martin Luther University Halle-Wittenberg, 06120 Halle (Saale), Germany.
- Interdisciplinary Center of Materials Science, Martin Luther University Halle-Wittenberg, 06120 Halle (Saale), Germany
- Laboratory of Biomedical Nanotechnologies, Institute of Bionic Technologies and Engineering, I.M. Sechenov First Moscow State University, 119991, Trubetskaya street 8, Moscow, Russian Federation
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SAWAN MARWA, REDA HILAL, SAAD NADINE, HAMADEH ABBASS, BIN SUN, NASSAR GEORGES. NUMERICAL APPROACH TO QUANTIFY AND CHARACTERIZE INTERFACE MECHANICAL BEHAVIOR BETWEEN THIN LAYER SUBSTRATE AND BIOLOGICAL TISSUES. J MECH MED BIOL 2021; 21:2150026. [DOI: 10.1142/s0219519421500263] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 01/22/2025]
Abstract
In this paper, we investigate the behavior of biological tissues (skin) coupled to a substrate (sensor) based on a numerical model taking into account the relationship between strain/stress components at the interface. Based on this study, we understand and quantify the most appropriate biomechanical factors in order to optimize sensor/biological tissue interface conditions. A micromechanical description based on a mathematical formulation has been developed to evaluate the biomechanical behavior provided by a 2D viscoelastic model of Kelvin–Voigt. The results show a spatio-temporal law of tissue motion highlighting the need for an optimized interface for reliable data transmission in the case of connected device in a dynamic movement or in the manufacturing of intelligent and reactive prosthesis device.
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Affiliation(s)
- MARWA SAWAN
- Opto-Acousto-Electronic Department, Institute of Electronics, Microelectronics and Nanotechnology, University Polytechnic Hauts-De-France, Campus Mont Houy 59313, Valenciennes Cedex 9, France
| | - HILAL REDA
- Faculty of Engineering, Lebanese University, Hadath Campus, Lebanon
| | - NADINE SAAD
- Faculty of Science, Lebanese University, Fanar Campus, Lebanon
| | - ABBASS HAMADEH
- Faculty of Engineering, Lebanese University, Hadath Campus, Lebanon
| | - SUN BIN
- College of Physics, QingDao, P. R. China
| | - GEORGES NASSAR
- Opto-Acousto-Electronic Department, Institute of Electronics, Microelectronics and Nanotechnology, University Polytechnic Hauts-De-France, Campus Mont Houy 59313, Valenciennes Cedex 9, France
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Di Gregorio E, Bitonto V, Baroni S, Stefania R, Aime S, Broche LM, Senn N, Ross PJ, Lurie DJ, Geninatti Crich S. Monitoring tissue implants by field-cycling 1H-MRI via the detection of changes in the 14N-quadrupolar-peak from imidazole moieties incorporated in a "smart" scaffold material. J Mater Chem B 2021; 9:4863-4872. [PMID: 34095943 DOI: 10.1039/d1tb00775k] [Citation(s) in RCA: 4] [Impact Index Per Article: 1.0] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/21/2022]
Abstract
This study is focused on the development of innovative sensors to non-invasively monitor the tissue implant status by Fast-Field-Cycling Magnetic Resonance Imaging (FFC-MRI). These sensors are based on oligo-histidine moieties that are conjugated to PLGA polymers representing the structural matrix for cells hosting scaffolds. The presence of 14N atoms of histidine causes a quadrupolar relaxation enhancement (also called Quadrupolar Peak, QP) at 1.39 MHz. This QP falls at a frequency well distinct from the QPs generated by endogenous semisolid proteins. The relaxation enhancement is pH dependent in the range 6.5-7.5, thus it acts as a reporter of the scaffold integrity as it progressively degrades upon lowering the microenvironmental pH. The ability of this new sensors to generate contrast in an image obtained at 1.39 MHz on a FFC-MRI scanner is assessed. A good biocompatibility of the histidine-containing scaffolds is observed after its surgical implantation in healthy mice. Over time the scaffold is colonized by endogenous fibroblasts and this process is accompanied by a progressive decrease of the intensity of the relaxation peak. In respect to the clinically used contrast agents this material has the advantage of generating contrast without the use of potentially toxic paramagnetic metal ions.
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Affiliation(s)
- Enza Di Gregorio
- Department of Molecular Biotechnology and Health Sciences, University of Torino, via Nizza 52, Torino, Italy.
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Nakayama M, Kanno T, Takahashi H, Kikuchi A, Yamato M, Okano T. Terminal cationization of poly( N-isopropylacrylamide) brush surfaces facilitates efficient thermoresponsive control of cell adhesion and detachment. SCIENCE AND TECHNOLOGY OF ADVANCED MATERIALS 2021; 22:481-493. [PMID: 34211335 PMCID: PMC8221160 DOI: 10.1080/14686996.2021.1929464] [Citation(s) in RCA: 9] [Impact Index Per Article: 2.3] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 05/04/2023]
Abstract
A variety of poly(N-isopropylacrylamide) (PIPAAm)-grafted surfaces have been reported for temperature-controlled cell adhesion/detachment. However, the surfaces reported to date need further improvement to achieve good outcomes for both cell adhesion and detachment, which are inherently contradictory behaviors. This study investigated the effects of terminal cationization and length of grafted PIPAAm chains on temperature-dependent cell behavior. PIPAAm brushes with three chain lengths were constructed on glass coverslips via surface-initiated reversible addition-fragmentation chain transfer (RAFT) polymerization. Terminal substitution of the grafted PIPAAm chains with either monocationic trimethylammonium or nonionic isopropyl moieties was performed through the reduction of terminal RAFT-related groups and subsequent thiol-ene reaction with the corresponding acrylamide derivatives. Although the thermoresponsive properties of the PIPAAm brush surfaces were scarcely affected by the terminal functional moiety, the zeta potentials of the cationized PIPAAm surfaces were higher than those of the nonionized ones, both below and above the phase transition temperature of PIPAAm (30°C). When bovine endothelial cells were cultured on each surface at 37°C, the number of adherent cells decreased with longer PIPAAm. Notably, cell adhesion on the cationized PIPAAm surfaces was higher than that on the nonionized surfaces. This terminal effect on cell adhesion gradually weakened with increasing PIPAAm length. In particular, long-chain PIPAAm brushes virtually showed cell repellency even at 37°C, regardless of the termini. Interestingly, moderately long-chain PIPAAm brushes promoted cell detachment at 20°C, with negligible terminal electrostatic interruption. Consequently, both cell adhesion and detachment were successfully improved by choosing an appropriate PIPAAm length with terminal cationization.
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Affiliation(s)
- Masamichi Nakayama
- Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, Shinjuku, Japan
| | - Tomonori Kanno
- Department of Materials Science and Technology, Graduate School of Advanced Engineering, Tokyo University of Science, Katsushika, Japan
| | - Hironobu Takahashi
- Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, Shinjuku, Japan
| | - Akihiko Kikuchi
- Department of Materials Science and Technology, Graduate School of Advanced Engineering, Tokyo University of Science, Katsushika, Japan
| | - Masayuki Yamato
- Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, Shinjuku, Japan
| | - Teruo Okano
- Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, Shinjuku, Japan
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Wang W, Chen JX, Hou Y, Bartolo P, Chiang WH. Investigations of Graphene and Nitrogen-Doped Graphene Enhanced Polycaprolactone 3D Scaffolds for Bone Tissue Engineering. NANOMATERIALS (BASEL, SWITZERLAND) 2021; 11:929. [PMID: 33917418 PMCID: PMC8067503 DOI: 10.3390/nano11040929] [Citation(s) in RCA: 10] [Impact Index Per Article: 2.5] [Reference Citation Analysis] [Abstract] [Key Words] [Grants] [Track Full Text] [Download PDF] [Figures] [Subscribe] [Scholar Register] [Received: 03/12/2021] [Revised: 03/31/2021] [Accepted: 04/05/2021] [Indexed: 12/23/2022]
Abstract
Scaffolds play a key role in tissue engineering applications. In the case of bone tissue engineering, scaffolds are expected to provide both sufficient mechanical properties to withstand the physiological loads, and appropriate bioactivity to stimulate cell growth. In order to further enhance cell-cell signaling and cell-material interaction, electro-active scaffolds have been developed based on the use of electrically conductive biomaterials or blending electrically conductive fillers to non-conductive biomaterials. Graphene has been widely used as functioning filler for the fabrication of electro-active bone tissue engineering scaffolds, due to its high electrical conductivity and potential to enhance both mechanical and biological properties. Nitrogen-doped graphene, a unique form of graphene-derived nanomaterials, presents significantly higher electrical conductivity than pristine graphene, and better surface hydrophilicity while maintaining a similar mechanical property. This paper investigates the synthesis and use of high-performance nitrogen-doped graphene as a functional filler of poly(ɛ-caprolactone) (PCL) scaffolds enabling to develop the next generation of electro-active scaffolds. Compared to PCL scaffolds and PCL/graphene scaffolds, these novel scaffolds present improved in vitro biological performance.
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Affiliation(s)
- Weiguang Wang
- Department of Mechanical, Aerospace and Civil Engineering, School of Engineering, Faculty of Science and Engineering, The University of Manchester, Manchester M13 9PL, UK; (Y.H.); (P.B.)
| | - Jun-Xiang Chen
- Department of Chemical Engineering, National Taiwan University of Science and Technology, Taipei E2-514, Taiwan;
| | - Yanhao Hou
- Department of Mechanical, Aerospace and Civil Engineering, School of Engineering, Faculty of Science and Engineering, The University of Manchester, Manchester M13 9PL, UK; (Y.H.); (P.B.)
| | - Paulo Bartolo
- Department of Mechanical, Aerospace and Civil Engineering, School of Engineering, Faculty of Science and Engineering, The University of Manchester, Manchester M13 9PL, UK; (Y.H.); (P.B.)
| | - Wei-Hung Chiang
- Department of Chemical Engineering, National Taiwan University of Science and Technology, Taipei E2-514, Taiwan;
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Yang Y, Xu LP, Zhang X, Wang S. Bioinspired wettable-nonwettable micropatterns for emerging applications. J Mater Chem B 2021; 8:8101-8115. [PMID: 32785360 DOI: 10.1039/d0tb01382j] [Citation(s) in RCA: 14] [Impact Index Per Article: 3.5] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 12/27/2022]
Abstract
Superhydrophilic and superhydrophobic surfaces are prevalent in nature and have received tremendous attention due to their importance in both fundamental research and practical applications. With the high interdisciplinary research and great development of microfabrication techniques, artificial wettable-nonwettable micropatterns inspired by the water-collection behavior of desert beetles have been successfully fabricated. A combination of the two extreme states of superhydrophilicity and superhydrophobicity on the same surface precisely, wettable-nonwettable micropatterns possess unique functionalities, such as controllable superwetting, anisotropic wetting, oriented adhesion, and other properties. In this review, we briefly describe the methods for fabricating wettable-nonwettable patterns, including self-assembly, electrodeposition, inkjet printing, and photolithography. We also highlight some of the emerging applications such as water collection, controllable bioadhesion, cell arrays, microreactors, printing techniques, and biosensors combined with various detection methods. Finally, the current challenges and prospects of this renascent and rapidly developing field are proposed and discussed.
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Affiliation(s)
- Yuemeng Yang
- Research Center for Bioengineering and Sensing Technology, University of Science and Technology Beijing, Beijing 100083, China.
| | - Li-Ping Xu
- Research Center for Bioengineering and Sensing Technology, University of Science and Technology Beijing, Beijing 100083, China.
| | - Xueji Zhang
- Research Center for Bioengineering and Sensing Technology, University of Science and Technology Beijing, Beijing 100083, China. and School of Biomedical Engineering, Health Science Center, Shenzhen University, Shenzhen 518060, Guangdong, China
| | - Shutao Wang
- CAS Key Laboratory of Bio-inspired Materials and Interfacial Science, CAS Center for Excellence in Nanoscience, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, China
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Horowitz JA, Zhong X, DePalma SJ, Ward Rashidi MR, Baker BM, Lahann J, Forrest SR. Printable Organic Electronic Materials for Precisely Positioned Cell Attachment. LANGMUIR : THE ACS JOURNAL OF SURFACES AND COLLOIDS 2021; 37:1874-1881. [PMID: 33497243 PMCID: PMC9794193 DOI: 10.1021/acs.langmuir.0c03319] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [MESH Headings] [Grants] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 06/12/2023]
Abstract
Over the past 3 decades, there has been a vast expansion of research in both tissue engineering and organic electronics. Although the two fields have interacted little, the materials and fabrication technologies which have accompanied the rise of organic electronics offer the potential for innovation and translation if appropriately adapted to pattern biological materials for tissue engineering. In this work, we use two organic electronic materials as adhesion points on a biocompatible poly(p-xylylene) surface. The organic electronic materials are precisely deposited via vacuum thermal evaporation and organic vapor jet printing, the proven, scalable processes used in the manufacture of organic electronic devices. The small molecular-weight organics prevent the subsequent growth of antifouling polyethylene glycol methacrylate polymer brushes that grow within the interstices between the molecular patches, rendering these background areas both protein and cell resistant. Last, fibronectin attaches to the molecular patches, allowing for the selective adhesion of fibroblasts. The process is simple, reproducible, and promotes a high yield of cell attachment to the targeted sites, demonstrating that biocompatible organic small-molecule materials can pattern cells at the microscale, utilizing techniques widely used in electronic device fabrication.
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Affiliation(s)
- Jeffrey A Horowitz
- Department of Electrical and Computer Engineering, University of Michigan, 1301 Beal Avenue, Ann Arbor, Michigan 48109, United States
| | - Xiaoyang Zhong
- Department of Materials Science and Engineering, University of Michigan, 2800 Plymouth Rd, Ann Arbor, Michigan 48109, United States
- Biointerfaces Institute, University of Michigan, 2800 Plymouth Rd, Ann Arbor, Michigan 48109, United States
| | - Samuel J DePalma
- Department of Biomedical Engineering, University of Michigan, 2200 Bonisteel Blvd, Ann Arbor, Michigan 48109, United States
| | - Maria R Ward Rashidi
- Department of Materials Science and Engineering, University of Michigan, 2800 Plymouth Rd, Ann Arbor, Michigan 48109, United States
- Biointerfaces Institute, University of Michigan, 2800 Plymouth Rd, Ann Arbor, Michigan 48109, United States
| | - Brendon M Baker
- Department of Biomedical Engineering, University of Michigan, 2200 Bonisteel Blvd, Ann Arbor, Michigan 48109, United States
| | - Joerg Lahann
- Department of Materials Science and Engineering, University of Michigan, 2800 Plymouth Rd, Ann Arbor, Michigan 48109, United States
- Biointerfaces Institute, University of Michigan, 2800 Plymouth Rd, Ann Arbor, Michigan 48109, United States
| | - Stephen R Forrest
- Department of Electrical and Computer Engineering, University of Michigan, 1301 Beal Avenue, Ann Arbor, Michigan 48109, United States
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Costa de Oliveira Souza CM, de Souza CF, Mogharbel BF, Irioda AC, Cavichiolo Franco CR, Sierakowski MR, Athayde Teixeira de Carvalho K. Nanostructured Cellulose-Gellan-Xyloglucan-Lysozyme Dressing Seeded with Mesenchymal Stem Cells for Deep Second-Degree Burn Treatment. Int J Nanomedicine 2021; 16:833-850. [PMID: 33584096 PMCID: PMC7875079 DOI: 10.2147/ijn.s289868] [Citation(s) in RCA: 8] [Impact Index Per Article: 2.0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 11/05/2020] [Accepted: 01/06/2021] [Indexed: 12/12/2022] Open
Abstract
PURPOSE In deep burns, wound contraction and hypertrophic scar formation can generate functional derangement and debilitation of the affected part. In order to improve the quality of healing in deep second-degree burns, we developed a new treatment in a preclinical model using nanostructured membranes seeded with mesenchymal stem cells (MSCs). METHODS Membranes were obtained by reconstitution of bacterial cellulose (reconstituted membrane [RM]) and produced by a dry-cast process, then RM was incorporated with 10% tamarind xyloglucan plus gellan gum 1:1 and 10% lysozyme (RMGT-LZ) and with 10% gellan gum and 10% lysozyme (RMG-LZ). Membrane hydrophobic/hydrophilic characteristics were investigated by static/dynamic contact-angle measurements. They were cultivated with MSCs, and cell adhesion, proliferation, and migration capacity was analyzed with MTT assays. Morphological and topographic characteristics were analyzed by scanning electron microscopy. MSC patterns in flow cytometry and differentiation into adipocytes and osteocytes were checked. In vivo assays used RMG-LZ and RMGT-LZ (with and without MSCs) in Rattus norvegicus rats submitted to burn protocol, and histological sections and collagen deposits were analyzed and immunocytochemistry assay performed. RESULTS In vitro results demonstrated carboxyl and amine groups made the membranes moderately hydrophobic and xyloglucan inclusion decreased wettability, favoring MSC adhesion, proliferation, and differentiation. In vivo, we obtained 40% and 60% reduction in acute/chronic inflammatory infiltrates, 96% decrease in injury area, increased vascular proliferation and collagen deposition, and complete epithelialization after 30 days. MSCs were detected in burned tissue, confirming they had homed and proliferated in vivo. CONCLUSION Nanostructured cellulose-gellan-xyloglucan-lysozyme dressings, especially when seeded with MSCs, improved deep second-degree burn regeneration.
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Affiliation(s)
- Carolina Maria Costa de Oliveira Souza
- Stem Cell Research Laboratory, Cell Therapy and Biotechnology in Regenerative Medicine Department, Pequeno Príncipe Faculties and the Pelé Pequeno Príncipe Research Institute, Curitiba, Paraná, Brazil
| | - Clayton Fernandes de Souza
- Chemistry Undergraduate Program, School of Education and Humanities, Pontifícia Universidade Católica Do Paraná, Curitiba, Paraná, Brazil
- BioPol, Chemistry Department, Federal University of Paraná, Curitiba, Paraná, Brazil
| | - Bassam Felipe Mogharbel
- Stem Cell Research Laboratory, Cell Therapy and Biotechnology in Regenerative Medicine Department, Pequeno Príncipe Faculties and the Pelé Pequeno Príncipe Research Institute, Curitiba, Paraná, Brazil
| | - Ana Carolina Irioda
- Stem Cell Research Laboratory, Cell Therapy and Biotechnology in Regenerative Medicine Department, Pequeno Príncipe Faculties and the Pelé Pequeno Príncipe Research Institute, Curitiba, Paraná, Brazil
| | | | | | - Katherine Athayde Teixeira de Carvalho
- Stem Cell Research Laboratory, Cell Therapy and Biotechnology in Regenerative Medicine Department, Pequeno Príncipe Faculties and the Pelé Pequeno Príncipe Research Institute, Curitiba, Paraná, Brazil
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