Survival in oesophago-gastric cancer (OGC) is poor due to early diagnostic challenges. Non-invasive risk stratification may identify susceptible patients with pre-malignant or benign disease. Following diagnostic confirmation with endoscopic biopsy, early OGC may be treated sooner. Mucins are transmembrane glycoproteins implicated in OGC with potential use as biomarkers of malignant transformation. This systematic review defines the role of mucins in OGC diagnosis. A literature search of MEDLINE, Web of Science, Embase and Cochrane databases was performed following PRISMA protocols for studies published January 1960-December 2022. Demographic data and data on mucin sampling and analysis methods were extracted. The review included 124 studies (n = 11,386 patients). Gastric adenocarcinoma (GAc) was the commonest OG malignancy (n = 101) followed by oesophageal adenocarcinoma (OAc, n = 24) and squamous cell carcinoma (OSqCc, n = 10). Mucins MUC1, MUC2, MUC5AC and MUC6 were the most frequently implicated. High MUC1 expression correlated with poorer prognosis and metastases in OSqCc. MUC2 expression decreases during progression from healthy mucosa to OAc, causing reduced protection from gastric acid. MUC5AC was upregulated, and MUC6 downregulated in GAc. Mucin expression varies in OGC; changes may be epigenetic or mutational. Profiling upper GI mucin expression in OGC, with pre-malignant, benign and healthy controls may identify potential early diagnostic biomarkers.

In biomaterials, a substantial amount of research has been placed on the mechanical properties of biomolecules and their interactions with body fluids. Bovine serum albumin (BSA) is a widely studied model protein, while bovine submaxillary gland mucin (BSM) is another cow-derived protein frequently employed in research. Films were examined with contact resonance atomic force microscopy (CR-AFM), and the results showed that the mechanical characteristics of the films were affected by the relative humidity. We quantitatively analyze the viscoelasticity of these proteins after they have been subjected to humidity by measuring the resonance frequency and quality factor. The findings indicate that prolonged humidity exposure has a different effect on the mechanical properties of BSA and BSM films. The results show that after exposure to humidity, the resonance peaks of BSA shift to the left, indicating stiffness, while those of BSM shift to the right, indicating hydration. Moreover, BSM's hydration is caused by relative humidity, leading to a constant increase in resonance frequency and material softness. Contrarily, BSA showed a decrease in contact resonance frequency due to ongoing strain-induced deformation, indicating increased material stiffness. The findings have significance for the design and development of biomaterials for a variety of applications, such as the delivery of drugs, the engineering of tissue, and the development of biosensors. Our research demonstrates that CR-AFM has the potential to become a non-invasive and sensitive method that can be used to characterize the mechanical characteristics of biomolecules and their interactions with bodily fluids.

One of the essential functions of mucous gel is protection of tissues against dehydration. The effect of hydration on the structural and thermodynamic properties of pig gastric mucin (PGM) and bovine submaxillary gland mucin (BSM) have been studied using atomic force microscopy (AFM), sorption, and differential scanning calorimetry (DSC). The analysis of sorption isotherms shows the higher water sorption capacity of PGM compared to BSM at RH levels lower than about 78%. The value of the hydration enthalpy at zero water content at 25 °C for both biopolymers is about -20 kJ/mol. Glass transitions of BSM and PGM occur at RH levels between 60 and 70% for both mucins. AFM indicates the presence of a dumbbell structure as well as a fiber-like structure in PGM samples. The experimental volume of the dry dumbbell molecule obtained by AFM is 3140 ± 340 nm(3). Using DSC data, the amount of nonfreezing water was calculated to be about 0.51 g/g of PGM. The phase diagram of PGM demonstrates two regions of different Tg: dependent and independent of hydration levels. In particular, at mucin concentrations from 0 to 67 wt %, the glass transition occurs at a constant temperature of about -15 °C. At higher concentrations of mucin, Tg is increasing with increasing mucin concentrations.