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Zheng W, Cheng Y, Shen H, Liu L, Hu W, Qian H. Research Progress on Antibacterial Applications of Bioactive Materials in Wound Infections: Design, Challenges, and Prospects. Adv Healthc Mater 2025; 14:e2405103. [PMID: 40114601 DOI: 10.1002/adhm.202405103] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Grants] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 01/14/2025] [Revised: 03/02/2025] [Indexed: 03/22/2025]
Abstract
Bacterial wound infections pose a significant threat to global health, exacerbated by the increase in multidrug-resistant bacteria (MDRB) and the formation of elastic biofilms. This review explores the transformative potential of bioactive materials in addressing these challenges, focusing on their design, mechanisms of action, and therapeutic effects. In vivo, bioactive materials are designed to respond to unique bacterial microenvironment (BME), utilizing enzyme activity, controlled gas release, surface functionalization, and immune regulation to combat infections. In vitro, this review provides a comprehensive overview of the latest advances in the rational design of these materials, emphasizing the synergistic integration of structural modifications (such as size and morphology) with external physical stimuli (such as light, sound, electricity, magnetism, and force) to enhance antibacterial performance. Finally, the outstanding challenges and prospects in this rapidly evolving field are discussed.
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Affiliation(s)
- Wang Zheng
- Department of Clinical Pharmacology, The Second Hospital of Anhui Medical University, Anhui Medical University, Hefei, 230601, P. R. China
| | - Yuanfang Cheng
- Department of Critical Care Medicine, The First Affiliated Hospital of Anhui Medical University, Anhui Medical University, Hefei, 230032, P. R. China
| | - Hui Shen
- School of Biomedical Engineering, Anhui Provincial Institute of Translational Medicine, Anhui Medical University, Hefei, 230032, P. R. China
| | - Litao Liu
- School of Biomedical Engineering, Anhui Provincial Institute of Translational Medicine, Anhui Medical University, Hefei, 230032, P. R. China
| | - Wei Hu
- Department of Clinical Pharmacology, The Second Hospital of Anhui Medical University, Anhui Medical University, Hefei, 230601, P. R. China
| | - Haisheng Qian
- School of Biomedical Engineering, Anhui Provincial Institute of Translational Medicine, Anhui Medical University, Hefei, 230032, P. R. China
- Department of Clinical Pharmacology, The Second Hospital of Anhui Medical University, Anhui Medical University, Hefei, 230601, P. R. China
- Anhui Engineering Research Center for Medical Micro-Nano Devices, Anhui Medical University, Hefei, 230032, P. R. China
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Liu Y, Zhang H, He M, Li S, Xu Y, Zhen JB. Quaternized chitosan templated MoS 2 nanohybrids for photothermal-enhanced synergistic antibacterial therapy. Int J Biol Macromol 2025; 298:139525. [PMID: 39761887 DOI: 10.1016/j.ijbiomac.2025.139525] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 08/22/2024] [Revised: 12/02/2024] [Accepted: 01/03/2025] [Indexed: 02/02/2025]
Abstract
Bacterial infections have become a fatal issue for human health. The excessive use of antibiotics leads to bacterial resistance. It is of great importance to develop alternate antimicrobial nanomaterials for effective antibacterial therapy. Herein, we developed a simple one-step hydrothermal method to construct the antibacterial nanoplatform based on chitosan quaternary ammonium salt functionalized molybdenum disulfide nanohybrids (MoS2-QCS) with controllable morphology, surface composition, and structure. The photothermal performance of MoS2-QCS nanohybrids can be successfully optimized by regulating the morphology, surface composition, and structure by QCS during hydrothermal synthesis. The optimized MoS₂-QCS nanohybrids demonstrated satisfactory photothermal effects, excellent colloidal stability, and enhanced bacterial adhesion. In vitro experiments verified the synergistic antibacterial efficacy of MoS2-QCS nanohybrids, combining photothermal therapy with QCS to effectively inhibit both Gram-positive and Gram-negative bacteria. The nanohybrids exhibited excellent biocompatibility, indicating the suitability for biomedical applications. In vivo studies demonstrated their potent antibacterial activity against S. aureus, along with accelerated wound healing and enhanced tissue regeneration with minimal inflammatory response. The current work proposed a simple and effective strategy for precisely designing nanoplatforms with controllable morphology, surface composition, and structure for synergistic antimicrobial therapy. These results confirmed the great potential of tailored MoS2-QCS nanohybrids in effective synergistic antibacterial therapy.
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Affiliation(s)
- Yanjun Liu
- Department of Materials Engineering, Taiyuan Institute of Technology, Taiyuan 030008, China; SEM Bio-Engineering Technology Co., Ltd., Dalian 116600, China; School of Bioengineering, Dalian University of Technology, Dalian 116024, China.
| | - Henghui Zhang
- Department of Environment and Safety Engineering, Taiyuan Institute of Technology, Taiyuan 030008, China
| | - Maoyong He
- Department of Materials Engineering, Taiyuan Institute of Technology, Taiyuan 030008, China
| | - Shuying Li
- SEM Bio-Engineering Technology Co., Ltd., Dalian 116600, China
| | - Yongping Xu
- SEM Bio-Engineering Technology Co., Ltd., Dalian 116600, China; School of Bioengineering, Dalian University of Technology, Dalian 116024, China.
| | - Jian Bin Zhen
- Department of Materials Engineering, Taiyuan Institute of Technology, Taiyuan 030008, China.
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Zhang D, Kukkar D, Bhatt P, Kim KH, Kaur K, Wang J. Novel nanomaterials-based combating strategies against drug-resistant bacteria. Colloids Surf B Biointerfaces 2025; 248:114478. [PMID: 39778220 DOI: 10.1016/j.colsurfb.2024.114478] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 11/09/2024] [Revised: 12/22/2024] [Accepted: 12/24/2024] [Indexed: 01/11/2025]
Abstract
Numerous types of contemporary antibiotic treatment regimens have become ineffective with the increasing incidence of drug tolerance. As a result, it is pertinent to seek novel and innovative solutions such as antibacterial nanomaterials (NMs) for the prohibition and treatment of hazardous microbial infections. Unlike traditional antibiotics (e.g., penicillin and tetracycline), the unique physicochemical characteristics (e.g., size dependency) of NMs endow them with bacteriostatic and bactericidal potential. However, it is yet difficult to mechanistically predict or decipher the networks of molecular interaction (e.g., between NMs and the biological systems) and the subsequent immune responses. In light of such research gap, this review outlines various mechanisms accountable for the inception of drug tolerance in bacteria. It also delineates the primary factors governing the NMs-induced molecular mechanisms against microbes, specifically drug-resistant bacteria along with the various NM-based mechanisms of antibacterial activity. The review also explores future directions and prospects for NMs in combating drug-resistant bacteria, while addressing challenges to their commercial viability within the healthcare industry.
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Affiliation(s)
- Daohong Zhang
- Yantai Key Laboratory of Nanoscience and Technology for Prepared Food, Yantai Engineering Research Center of Green Food Processing and Quality Control, College of Food Engineering, Ludong University, Yantai, Shandong 264025, China
| | - Deepak Kukkar
- Department of Biotechnology, Chandigarh University, Gharuan, Mohali 140413, India; University Center for Research and Development, Chandigarh University, Gharuan, Mohali 140413, India.
| | - Poornima Bhatt
- Department of Biotechnology, Chandigarh University, Gharuan, Mohali 140413, India; University Center for Research and Development, Chandigarh University, Gharuan, Mohali 140413, India
| | - Ki-Hyun Kim
- Department of Civil and Environmental Engineering, Hanyang University, 222 Wangsimni-Ro, Seoul 04763, South Korea.
| | - Kamalpreet Kaur
- Department of Chemistry, Mata Gujri College, Fatehgarh Sahib, Punjab 140406, India
| | - Jianlong Wang
- College of Food Science and Engineering, Northwest A&F University, Yangling, Shaanxi 712100, China
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Zheng Y, Zhang T, Shao J, Du Y, Li Z, Zhang L, Gao J. Antibiotic-free responsive biomaterials for specific and targeted Helicobacter pylori eradication. J Control Release 2025; 379:708-729. [PMID: 39863021 DOI: 10.1016/j.jconrel.2025.01.054] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 08/03/2024] [Revised: 12/17/2024] [Accepted: 01/20/2025] [Indexed: 01/27/2025]
Abstract
Gastric cancer is highly correlated with Helicobacter pylori (H. pylori) infection. Approximately 50 % of the population worldwide is infected with H. pylori. However, current treatment regimens face severe challenges including drug resistance and gut microbiota disruption. An integrative treatment with slight negative influences on intestinal flora, conforming with concepts of integrative prevention of gastric cancer, is urgently needed. Non-antibiotic responsive biomaterials can respond to different stimuli, including pH, enzymes, light, ultrasound and magnetism, under which biomaterials are specifically activated to perform antibacterial capabilities, while neutral intestinal microenvironments differ from gastric microenvironments or inflammatory sites and have no or minimal irradiation via precisely controlled exogenous stimuli, which may not only overcome antibiotic resistance but also avoid gut microbiota disorders. First, the latest progress in responsive biomaterials against H. pylori without gut microbiome disturbance and their anti-H. pylori performances are profoundly summarized. Second, the mechanisms against planktonic bacteria, biofilms and intracellular bacteria are discussed respectively. Finally, the strategies of specific and targeted H. pylori elimination by responsive biomaterials are introduced. Additionally, the challenges and the focus of future research on translation into clinical application are fully proposed. Antibiotic-free responsive biomaterials for specific and targeted H. pylori eradication represent an innovative approach.
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Affiliation(s)
- Yating Zheng
- Changhai Clinical Research Unit, Shanghai Changhai Hospital, Naval Medical University, Shanghai 200433, China; Yangzhou Branch of Jiangsu Provincial Corps of Chinese People's Armed Police Force, Yangzhou 225007, Jiangsu, China
| | - Tinglin Zhang
- Changhai Clinical Research Unit, Shanghai Changhai Hospital, Naval Medical University, Shanghai 200433, China; Shanghai Key Laboratory of Nautical Medicine and Translation of Drugs and Medical Devices, Shanghai, China
| | - Juan Shao
- Yangzhou Branch of Jiangsu Provincial Corps of Chinese People's Armed Police Force, Yangzhou 225007, Jiangsu, China
| | - Yiqi Du
- Changhai Clinical Research Unit, Shanghai Changhai Hospital, Naval Medical University, Shanghai 200433, China; Department of Gastroenterology, Shanghai Changhai Hospital, Naval Medical University, Shanghai 200433, China; Shanghai Key Laboratory of Nautical Medicine and Translation of Drugs and Medical Devices, Shanghai, China
| | - Zhaoshen Li
- Changhai Clinical Research Unit, Shanghai Changhai Hospital, Naval Medical University, Shanghai 200433, China; Department of Gastroenterology, Shanghai Changhai Hospital, Naval Medical University, Shanghai 200433, China; Shanghai Key Laboratory of Nautical Medicine and Translation of Drugs and Medical Devices, Shanghai, China
| | - Li Zhang
- Changhai Clinical Research Unit, Shanghai Changhai Hospital, Naval Medical University, Shanghai 200433, China; Shanghai Key Laboratory of Nautical Medicine and Translation of Drugs and Medical Devices, Shanghai, China.
| | - Jie Gao
- Changhai Clinical Research Unit, Shanghai Changhai Hospital, Naval Medical University, Shanghai 200433, China; Shanghai Key Laboratory of Nautical Medicine and Translation of Drugs and Medical Devices, Shanghai, China.
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Javanmard Z, Pourhajibagher M, Bahador A. New strategies to enhance antimicrobial photo-sonodynamic therapy based on nanosensitizers against bacterial infections. Folia Microbiol (Praha) 2025; 70:55-70. [PMID: 39367131 DOI: 10.1007/s12223-024-01206-8] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 04/13/2024] [Accepted: 09/30/2024] [Indexed: 10/06/2024]
Abstract
The rapid evolution and spread of multidrug resistance among bacterial pathogens has significantly outpaced the development of new antibiotics, underscoring the urgent need for alternative therapies. Antimicrobial photodynamic therapy and antimicrobial sonodynamic therapy have emerged as promising treatments. Antimicrobial photodynamic therapy relies on the interaction between light and a photosensitizer to produce reactive oxygen species, which are highly cytotoxic to microorganisms, leading to their destruction without fostering resistance. Antimicrobial sonodynamic therapy, a novel variation, substitutes ultrasound for light to activate the sonosensitizers, expanding the therapeutic reach. To increase the efficiency of antimicrobial photodynamic therapy and antimicrobial sonodynamic therapy, the combination of these two methods, known as antimicrobial photo-sonodynamic therapy, is currently being explored and considered a promising approach. Recent advances, particularly in the application of nanomaterials, have further enhanced the efficacy of these therapies. Nanosensitizers, due to their improved reactive oxygen species generation and targeted delivery, offer significant advantages in overcoming the limitations of conventional sensitizers. These breakthroughs provide new avenues for treating bacterial infections, especially multidrug-resistant strains and biofilm-associated infections. Continued research, including comprehensive clinical studies, is crucial to optimizing nanomaterial-based antimicrobial photo-sonodynamic therapy for clinical use, ensuring their effectiveness in real-world applications.
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Affiliation(s)
- Zahra Javanmard
- Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
| | - Maryam Pourhajibagher
- Dental Research Center, Dentistry Research Institute, Tehran University of Medical Sciences, Tehran, Iran.
| | - Abbas Bahador
- Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
- Fellowship in Clinical Laboratory Sciences, BioHealth Lab, Tehran, Iran.
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Yu G, Wang J, Xiong Q, Xu Y, Xuan S, Leung KCF, Fang Q. Dipolar-hollowed α-Fe 2O 3@Au/Polydopamine nanospindle for photothermal-photodynamic coupling antibacterial and drug-delivery. Int J Biol Macromol 2024; 281:136615. [PMID: 39414200 DOI: 10.1016/j.ijbiomac.2024.136615] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 05/31/2024] [Revised: 10/12/2024] [Accepted: 10/14/2024] [Indexed: 10/18/2024]
Abstract
With the prevalence of drug-resistant bacteria and the waning effects of antibiotics, nanoplatform has become an effective strategy for fighting infections. This work reports a dipolar-hollowed α-Fe2O3@Au/Polydopamine (PDA) nanospindle which possesses both photothermal-photodynamic (PTT-PDT) coupling antibacterial and drug carrying performance. Firstly, the spindle type α-Fe2O3@Au/PDA particle was prepared by a simple one-step strategy and then the dipolar-hollow structure was obtained by controlling etching the inside α-Fe2O3 core with hydrochloric acid. After further loading the photosensitizer zinc phthalocyanine (ZnPc), the dipolar-hollowed α-Fe2O3@Au/PDA-ZnPc nanospindles were obtained. Owing to the dipolar-hollowed interior, the nanospindles are also effective in carrying antitumor drug doxorubicin (DOX) and shows a good drug loading-release behavior. The dipolar-hollowed α-Fe2O3@Au/PDA nanospindles exhibits a high antibacterial performance against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) under near-infrared (NIR) and Xenon lamp irradiation. When α-Fe2O3@Au/PDA-ZnPc nanospindles concentration was increased to 100 μg/mL, the antibacterial rate was close to 100 %. In comparison to the original α-Fe2O3@Au/PDA nanospindles, the product achieved a lower effective antibacterial temperature. This triple-mode therapy (PTT/PDT/Drug) provides an interesting design idea for anisotropic therapeutic nanoplatform which can be applied in low-temperature antibacterial and drug delivery.
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Affiliation(s)
- Guangjin Yu
- Department of Chirurgery, Affiliated Hospital 1, Anhui Medical University, Hefei 230032, PR China; Department of Hepatobiliary Surgery, Dongcheng branch of the First Affiliated Hospital of Anhui Medical University (Feidong People's Hospital), Hefei 230027, PR China
| | - Jing Wang
- School of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, PR China; Institute of Plasma Physics, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei 230031, PR China
| | - Qingshan Xiong
- School of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, PR China
| | - Yunqi Xu
- CAS Key Laboratory of Mechanical Behavior and Design of Materials, Department of Modern Mechanics, University of Science and Technology of China, Hefei 230027, PR China
| | - Shouhu Xuan
- CAS Key Laboratory of Mechanical Behavior and Design of Materials, Department of Modern Mechanics, University of Science and Technology of China, Hefei 230027, PR China; Anhui Provincial Key Laboratory of Aerosol Analysis, Regulation and Biological Effect, Hefei 230000, PR China.
| | - Ken Cham-Fai Leung
- State Key Laboratory of Environmental and Biological Analysis, Department of Chemistry, The Hong Kong Baptist University, Kowloon, Hong Kong.
| | - Qunling Fang
- School of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, PR China.
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Shi S, Han Y, Feng J, Shi J, Liu X, Fu B, Wang J, Zhang W, Duan J. Microenvironment-triggered cascade metal-polyphenolic nanozyme for ROS/NO synergistic hyperglycemic wound healing. Redox Biol 2024; 73:103217. [PMID: 38820984 PMCID: PMC11177078 DOI: 10.1016/j.redox.2024.103217] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Grants] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 02/07/2024] [Revised: 05/11/2024] [Accepted: 05/27/2024] [Indexed: 06/02/2024] Open
Abstract
Wound infection of hyperglycemic patient often has extended healing period and increased probability due to the high glucose level. However, achieving precise and safe therapy of the hyperglycemic wound with specific wound microenvironment (WME) remains a major challenge. Herein, a WME-activated smart L-Arg/GOx@TA-Fe (LGTF) nanozymatic system composed of generally recognized as safe (GRAS) compound is engineered. The nanozymatic system combining metal-polyphenol nanozyme (tannic acid-Fe3+, TA-Fe) and natural enzyme (glucose oxidase, GOx) can consume the high-concentration glucose, generating reactive oxygen species (ROS) and nitric oxide (NO) in situ to synergistically disinfect hyperglycemia wound. In addition, glucose consumption and gluconic acid generation can lower glucose level to promote wound healing and reduce the pH of WME to enhance the catalytic activities of the LGTF nanozymatic system. Thereby, low-dose LGTF can perform remarkable synergistic disinfection and healing effect towards hyperglycemic wound. The superior biosafety, high catalytic antibacterial and beneficial WME regulating capacity demonstrate this benign GRAS nanozymatic system is a promising therapeutic agent for hyperglycemic wound.
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Affiliation(s)
- Shuo Shi
- Shaanxi Key Laboratory of Natural Products & Chemical Biology, College of Chemistry & Pharmacy, Northwest A&F University, Yangling, 712100, Shaanxi, China; College of Food Science and Engineering, Northwest A&F University, Yangling, 712100, Shaanxi, China
| | - Yaru Han
- College of Food Science and Engineering, Northwest A&F University, Yangling, 712100, Shaanxi, China; Department of Mechanical Engineering, Columbia University, New York, NY, 10027, USA
| | - Jianxing Feng
- College of Food Science and Engineering, Northwest A&F University, Yangling, 712100, Shaanxi, China
| | - Jingru Shi
- Shaanxi Key Laboratory of Natural Products & Chemical Biology, College of Chemistry & Pharmacy, Northwest A&F University, Yangling, 712100, Shaanxi, China
| | - Xiaoling Liu
- Department of Mechanical Engineering, Columbia University, New York, NY, 10027, USA
| | - Bangfeng Fu
- College of Food Science and Engineering, Northwest A&F University, Yangling, 712100, Shaanxi, China
| | - Jianlong Wang
- College of Food Science and Engineering, Northwest A&F University, Yangling, 712100, Shaanxi, China.
| | - Wentao Zhang
- College of Food Science and Engineering, Northwest A&F University, Yangling, 712100, Shaanxi, China.
| | - Jinyou Duan
- Shaanxi Key Laboratory of Natural Products & Chemical Biology, College of Chemistry & Pharmacy, Northwest A&F University, Yangling, 712100, Shaanxi, China.
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Yang Y, Sun X, Wang S, Tang Z, Luo S, Shi J, Zhuo X, Zhu J, Zhang H, Kong X. Yolk-shelled silver nanowire@amorphous metal-organic framework for controlled drug delivery and light-promoting infected wound healing. Regen Biomater 2024; 11:rbae056. [PMID: 38845853 PMCID: PMC11153340 DOI: 10.1093/rb/rbae056] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Grants] [Track Full Text] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 02/07/2024] [Revised: 04/28/2024] [Accepted: 05/10/2024] [Indexed: 06/09/2024] Open
Abstract
Bacteria-infected wounds healing has been greatly hindered by antibiotic resistance and persistent inflammation. It is crucial to develop multifunctional nanocomposites that possess effective antibacterial properties and can simultaneously accelerate the wound healing process to overcome the above challenges. Herein, we prepared a yolk-shell structured Ag nanowires (NWs)@amorphous hollow ZIF-67 by etching ZIF-67 onto the Ag NWs for infected wound healing for the first time. The etched hollow structure of amorphous ZIF-67 in the nanocomposite makes it a promising platform for loading healing-promoting drugs. We extensively studied the antibacterial and healing-promoting properties of the curcumin (CCM)-loaded nanocomposite (Ag NWs@C-HZ67). Ag NWs, being noble metal materials with plasmonic effects, can absorb a broad range of natural light and convert it to thermal energy. This photothermal conversion further improves the release of antibacterial components and wound healing drugs when exposed to light. During the healing process of an infected wound, Ag and Co ions were released from Ag NWs@C-HZ67 upon direct contact with the wound exudate and under the influence of light irradiation. Simultaneously, the loaded CCM leaked out to repair the infected wound. The minimum inhibitory concentrations of the Ag NWs@C-HZ67 groups against Escherichia coli and Staphylococcus aureus bacteria decreased to 3 and 3 μg ml-1 when exposed to white light. Furthermore, an in vivo assessment of infected wound healing demonstrated that combining Ag NWs@C-HZ67 with light significantly accelerated the wound healing process, achieving 70% healing by the 6th day and almost complete healing by the 8th day. This advanced nanocomposite, consisting of components that possess antibacterial and growth-promoting properties, offers a safe, effective and clinically-translatable solution for accelerating the healing process of infected wounds.
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Affiliation(s)
- Yueyan Yang
- Institute for Smart Biomedical Materials, School of Materials Science & Engineering, Zhejiang Sci-Tech University, Hangzhou 310000, PR China
- Zhejiang-Mauritius Joint Research Center for Biomaterials and Tissue Engineering, Zhejiang Sci-Tech University, Hangzhou 310018, PR China
| | - Xu Sun
- Institute for Smart Biomedical Materials, School of Materials Science & Engineering, Zhejiang Sci-Tech University, Hangzhou 310000, PR China
- Zhejiang-Mauritius Joint Research Center for Biomaterials and Tissue Engineering, Zhejiang Sci-Tech University, Hangzhou 310018, PR China
| | - Shengyan Wang
- School of Science Engineering, The Chinese University of Hong Kong, Shenzhen, Guangdong 518172, PR China
| | - Zhe Tang
- Institute for Smart Biomedical Materials, School of Materials Science & Engineering, Zhejiang Sci-Tech University, Hangzhou 310000, PR China
- Zhejiang-Mauritius Joint Research Center for Biomaterials and Tissue Engineering, Zhejiang Sci-Tech University, Hangzhou 310018, PR China
| | - Siyuan Luo
- Institute for Smart Biomedical Materials, School of Materials Science & Engineering, Zhejiang Sci-Tech University, Hangzhou 310000, PR China
- Zhejiang-Mauritius Joint Research Center for Biomaterials and Tissue Engineering, Zhejiang Sci-Tech University, Hangzhou 310018, PR China
| | - Jianjun Shi
- Institute for Smart Biomedical Materials, School of Materials Science & Engineering, Zhejiang Sci-Tech University, Hangzhou 310000, PR China
- Zhejiang-Mauritius Joint Research Center for Biomaterials and Tissue Engineering, Zhejiang Sci-Tech University, Hangzhou 310018, PR China
| | - Xiaolu Zhuo
- School of Science Engineering, The Chinese University of Hong Kong, Shenzhen, Guangdong 518172, PR China
| | - Jinjin Zhu
- Department of Orthopaedic Surgery, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine & Key Laboratory of Musculoskeletal System Degeneration and Regeneration Translational Research of Zhejiang, Hangzhou 310016, PR China
| | - Han Zhang
- Institute for Smart Biomedical Materials, School of Materials Science & Engineering, Zhejiang Sci-Tech University, Hangzhou 310000, PR China
- Zhejiang-Mauritius Joint Research Center for Biomaterials and Tissue Engineering, Zhejiang Sci-Tech University, Hangzhou 310018, PR China
| | - Xiangdong Kong
- Institute for Smart Biomedical Materials, School of Materials Science & Engineering, Zhejiang Sci-Tech University, Hangzhou 310000, PR China
- Zhejiang-Mauritius Joint Research Center for Biomaterials and Tissue Engineering, Zhejiang Sci-Tech University, Hangzhou 310018, PR China
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Cheng JH, Du R, Sun DW. Regulating bacterial biofilms in food and biomedicine: unraveling mechanisms and Innovating strategies. Crit Rev Food Sci Nutr 2024; 65:1894-1910. [PMID: 38384205 DOI: 10.1080/10408398.2024.2312539] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 02/23/2024]
Abstract
Bacterial biofilm has brought a lot of intractable problems in food and biomedicine areas. Conventional biofilm control mainly focuses on inactivation and removal of biofilm. However, with robust construction and enhanced resistance, the established biofilm is extremely difficult to eradicate. According to the mechanism of biofilm development, biofilm formation can be modulated by intervening in the key factors and regulatory systems. Therefore, regulation of biofilm formation has been proposed as an alternative way for effective biofilm control. This review aims to provide insights into the regulation of biofilm formation in food and biomedicine. The underlying mechanisms for early-stage biofilm establishment are summarized based on the key factors and correlated regulatory networks. Recent developments and applications of novel regulatory strategies such as anti/pro-biofilm agents, nanomaterials, functionalized surface materials and physical strategies are also discussed. The current review indicates that these innovative methods have contributed to effective biofilm control in a smart, safe and eco-friendly way. However, standard methodology for regulating biofilm formation in practical use is still missing. As biofilm formation in real-world systems could be far more complicated, further studies and interdisciplinary collaboration are still needed for simulation and experiments in the industry and other open systems.
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Affiliation(s)
- Jun-Hu Cheng
- School of Food Science and Engineering, South China University of Technology, Guangzhou, China
- Academy of Contemporary Food Engineering, South China University of Technology, Guangzhou Higher Education Mega Centre, Guangzhou, China
- Engineering and Technological Research Centre of Guangdong Province on Intelligent Sensing and Process Control of Cold Chain Foods, & Guangdong Province Engineering Laboratory for Intelligent Cold Chain Logistics Equipment for Agricultural Products, Guangzhou Higher Education Mega Centre, Guangzhou, China
| | - Rong Du
- School of Food Science and Engineering, South China University of Technology, Guangzhou, China
- Academy of Contemporary Food Engineering, South China University of Technology, Guangzhou Higher Education Mega Centre, Guangzhou, China
- Engineering and Technological Research Centre of Guangdong Province on Intelligent Sensing and Process Control of Cold Chain Foods, & Guangdong Province Engineering Laboratory for Intelligent Cold Chain Logistics Equipment for Agricultural Products, Guangzhou Higher Education Mega Centre, Guangzhou, China
| | - Da-Wen Sun
- School of Food Science and Engineering, South China University of Technology, Guangzhou, China
- Academy of Contemporary Food Engineering, South China University of Technology, Guangzhou Higher Education Mega Centre, Guangzhou, China
- Engineering and Technological Research Centre of Guangdong Province on Intelligent Sensing and Process Control of Cold Chain Foods, & Guangdong Province Engineering Laboratory for Intelligent Cold Chain Logistics Equipment for Agricultural Products, Guangzhou Higher Education Mega Centre, Guangzhou, China
- Food Refrigeration and Computerized Food Technology (FRCFT), Agriculture and Food Science Centre, University College Dublin, National University of Ireland, Dublin 4, Ireland
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Finina BF, Mersha AK. Nano-enabled antimicrobial thin films: design and mechanism of action. RSC Adv 2024; 14:5290-5308. [PMID: 38357038 PMCID: PMC10866018 DOI: 10.1039/d3ra07884a] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Track Full Text] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 11/17/2023] [Accepted: 02/02/2024] [Indexed: 02/16/2024] Open
Abstract
Antimicrobial thin films are types of protective coatings that are applied to surfaces such as medical devices, food packaging materials, water-resistant coatings, and other systems. These films prevent and reduce the spread of microbial organisms, including bacteria, fungi, and viruses. Antimicrobial thin films can be prepared from a variety of nanostructured materials including metal nanoparticles, metal oxides, plant materials, enzymes, bacteriocins and polymers. Their antimicrobial mechanism varies mostly based on the types of active agents from which the film is made of. Antimicrobial thin films are becoming increasingly popular microbial treatment methods due to their advantages such as enhanced stability, reduced toxicity levels, extended effectiveness over time and broad spectrum antimicrobial action without side effects on human health or the environment. This popularity and enhanced performance is mainly due to the extended possibility of film designs. Thin films offer convenient formulation methods which makes them suitable for commercial practices aiming at high turnover rates along with residential applications requiring frequent application cycles. This review focuses on recent developments in the possible processing methods and design approaches for assembling the various types of antimicrobial materials into nanostructured thin film-based delivery systems, along with mechanisms of action against microbes.
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Affiliation(s)
- Bilisuma Fekadu Finina
- Department of Industrial Chemistry, Addis Ababa Science and Technology University Addis Ababa Ethiopia
- Department of Chemistry, Kotebe University of Education Addis Ababa Ethiopia
| | - Anteneh Kindu Mersha
- Department of Industrial Chemistry, Addis Ababa Science and Technology University Addis Ababa Ethiopia
- Nanotechnology Center of Excellence, Addis Ababa Science and Technology University Addis Ababa Ethiopia
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Rawat N, Ahmad N, Raturi P, Singhvi N, Sahai N, Kothiyal P. Nanobiomaterials: exploring mechanistic roles in combating microbial infections and cancer. DISCOVER NANO 2023; 18:158. [PMID: 38123864 PMCID: PMC10733259 DOI: 10.1186/s11671-023-03946-x] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Subscribe] [Scholar Register] [Received: 10/02/2023] [Accepted: 12/16/2023] [Indexed: 12/23/2023]
Abstract
The initiation of the "nanotechnology era" within the past decade has been prominently marked by advancements in biomaterials. This intersection has opened up numerous possibilities for enhancing the detection, diagnosis, and treatment of various illnesses by leveraging the synergy between biomaterials and nanotechnology. The term "nano biomaterials" referring to biomaterials featuring constituent or surface feature sizes below 100 nm, presents a realm of extraordinary materials endowed with unique structures and properties. Beyond addressing common biomedical challenges, these nano biomaterials contribute unprecedented insights and principles that enrich our understanding of biology, medicine, and materials science. A critical evaluation of recent technological progress in employing biomaterials in medicine is essential, along with an exploration of potential future trends. Nanotechnology breakthroughs have yielded novel surfaces, materials, and configurations with notable applications in the biomedical domain. The integration of nanotechnology has already begun to enhance traditional biomedical practices across diverse fields such as tissue engineering, intelligent systems, the utilization of nanocomposites in implant design, controlled release systems, biosensors, and more. This mini review encapsulates insights into biomaterials, encompassing their types, synthesis methods, and the roles of organic and inorganic nanoparticles, elucidating their mechanisms of action. Furthermore, the focus is squarely placed on nano biomaterials and their versatile applications, with a particular emphasis on their roles in anticancer and antimicrobial interventions. This review underscores the dynamic landscape of nanotechnology, envisioning a future where nano biomaterials play a pivotal role in advancing medical applications, particularly in combating cancer and microbial infections.
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Affiliation(s)
- Neha Rawat
- School of Allied Sciences, Dev Bhoomi Uttarakhand University, Dehradun, 248007, India
| | - Nabeel Ahmad
- School of Allied Sciences, Dev Bhoomi Uttarakhand University, Dehradun, 248007, India.
| | - Pratishtha Raturi
- School of Allied Sciences, Dev Bhoomi Uttarakhand University, Dehradun, 248007, India
| | - Nirjara Singhvi
- School of Allied Sciences, Dev Bhoomi Uttarakhand University, Dehradun, 248007, India
| | - Nitin Sahai
- 3D Printing and Visualization Center, University of Pecs, Boszorkany str. 2, Pecs, Hungary
- Departmnet of Biomedical Engineering, North Eastern Hill University (Central University), Shillong, India
| | - Preeti Kothiyal
- School of Pharmacy and Research, Dev Bhoomi Uttarakhand University, Dehradun, 248007, India
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12
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Busila M, Musat V, Alexandru P, Romanitan C, Brincoveanu O, Tucureanu V, Mihalache I, Iancu AV, Dediu V. Antibacterial and Photocatalytic Activity of ZnO/Au and ZnO/Ag Nanocomposites. Int J Mol Sci 2023; 24:16939. [PMID: 38069261 PMCID: PMC10706707 DOI: 10.3390/ijms242316939] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Grants] [Track Full Text] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 10/26/2023] [Revised: 11/25/2023] [Accepted: 11/27/2023] [Indexed: 12/18/2023] Open
Abstract
The use of a combination of nanoparticles as antimicrobial agents can be one strategy to overcome the tendency of microbes to become resistant to antibiotic action. Also, the optimization of nano-photocatalysts to efficiently remove persistent pollutants from wastewater is a hot topic. In this study, two composites ZnO/Au (1% wt.) and ZnO/Ag (1% wt.) were synthesized by simple aqueous solution methods. The structure and morphology of the r nanocomposites were analyzed by structural and optical characterization methods. The formation of AuNPs and AgNPs in these experiments was also discussed. The antimicrobial properties of ZnO, ZnO/Au, and ZnO/Ag nanomaterials were investigated against Gram-negative bacteria (Pseudomonas aeruginosa) and Gram-positive bacteria (Staphylococcus aureus). The results showed an increase of 80% in the antimicrobial activity of ZnO/Au against Pseudomonas aeruginosa compared with 30% in the case of ZnO/Ag. Similarly, in the case of the S. aureus strain tests, ZnO/Au increased the antimicrobial activity by 55% and ZnO/Ag by 33%. The photocatalytic tests indicated an improvement in the photocatalytic degradation of methylene blue (MB) under UV irradiation using ZnO/Au and ZnO/Ag nanocomposites compared to bare ZnO. The photocatalytic degradation efficiency of ZnO after 60 min of UV irradiation was ∼83%, while the addition of AuNPs enhanced the degradation rate to ∼95% (ZP2), and AgNP presence enhanced the efficiency to ∼98%. The introduction of noble metallic nanoparticles into the ZnO matrix proved to be an effective strategy to increase their antimicrobial activity against P. aeruginosa and S. aureus, and their photocatalytic activity was evaluated through the degradation of MB dye. Comparing the enhancing effects of Au and Ag, it was found that ZnO/Au was a better antimicrobial agent while ZnO/Ag was a more effective photocatalyst under UV irradiation.
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Affiliation(s)
- Mariana Busila
- Centre of Nanostructures and Functional Materials-CNMF, Faculty of Engineering, “Dunarea de Jos” University of Galati, Romania, Domneasca Street 111, 800201 Galati, Romania (P.A.)
| | - Viorica Musat
- Centre of Nanostructures and Functional Materials-CNMF, Faculty of Engineering, “Dunarea de Jos” University of Galati, Romania, Domneasca Street 111, 800201 Galati, Romania (P.A.)
| | - Petrica Alexandru
- Centre of Nanostructures and Functional Materials-CNMF, Faculty of Engineering, “Dunarea de Jos” University of Galati, Romania, Domneasca Street 111, 800201 Galati, Romania (P.A.)
| | - Cosmin Romanitan
- National Research and Development Institute in Microtechnologies–IMT Bucharest, 126A Erou Iancu Nicolae Street, 077190 Bucharest, Romania; (C.R.); (O.B.); (I.M.)
| | - Oana Brincoveanu
- National Research and Development Institute in Microtechnologies–IMT Bucharest, 126A Erou Iancu Nicolae Street, 077190 Bucharest, Romania; (C.R.); (O.B.); (I.M.)
| | - Vasilica Tucureanu
- National Research and Development Institute in Microtechnologies–IMT Bucharest, 126A Erou Iancu Nicolae Street, 077190 Bucharest, Romania; (C.R.); (O.B.); (I.M.)
| | - Iuliana Mihalache
- National Research and Development Institute in Microtechnologies–IMT Bucharest, 126A Erou Iancu Nicolae Street, 077190 Bucharest, Romania; (C.R.); (O.B.); (I.M.)
| | - Alina-Viorica Iancu
- Department of Morphological and Functional Sciences, Faculty of Medicine and Pharmacy, “Dunarea de Jos” University, 800008 Galati, Romania
- Medical Laboratory Department, Clinical Hospital for Infectious Diseases “Sf. Cuvioasa Parascheva”, 800179 Galati, Romania
| | - Violeta Dediu
- National Research and Development Institute in Microtechnologies–IMT Bucharest, 126A Erou Iancu Nicolae Street, 077190 Bucharest, Romania; (C.R.); (O.B.); (I.M.)
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13
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Li B, Liao Y, Su X, Chen S, Wang X, Shen B, Song H, Yue P. Powering mesoporous silica nanoparticles into bioactive nanoplatforms for antibacterial therapies: strategies and challenges. J Nanobiotechnology 2023; 21:325. [PMID: 37684605 PMCID: PMC10485977 DOI: 10.1186/s12951-023-02093-w] [Citation(s) in RCA: 14] [Impact Index Per Article: 7.0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Grants] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 07/18/2023] [Accepted: 08/31/2023] [Indexed: 09/10/2023] Open
Abstract
Bacterial infection has been a major threat to worldwide human health, in particular with the ever-increasing level of antimicrobial resistance. Given the complex microenvironment of bacterial infections, conventional use of antibiotics typically renders a low efficacy in infection control, thus calling for novel strategies for effective antibacterial therapies. As an excellent candidate for antibiotics delivery, mesoporous silica nanoparticles (MSNs) demonstrate unique physicochemical advantages in antibacterial therapies. Beyond the delivery capability, extensive efforts have been devoted in engineering MSNs to be bioactive to further synergize the therapeutic effect in infection control. In this review, we critically reviewed the essential properties of MSNs that benefit their antibacterial application, followed by a themed summary of strategies in manipulating MSNs into bioactive nanoplatforms for enhanced antibacterial therapies. The chemically functionalized platform, photo-synergized platform, physical antibacterial platform and targeting-directed platform are introduced in details, where the clinical translation challenges of these MSNs-based antibacterial nanoplatforms are briefly discussed afterwards. This review provides critical information of the emerging trend in turning bioinert MSNs into bioactive antibacterial agents, paving the way to inspire and translate novel MSNs-based nanotherapies in combating bacterial infection diseases.
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Affiliation(s)
- Biao Li
- Lab of Modern Preparation of TCM, Ministry of Education, Jiangxi University of Chinese Medicine, 1688 MEILING Avenue, Nanchang, 330004, China
| | - Yan Liao
- Lab of Modern Preparation of TCM, Ministry of Education, Jiangxi University of Chinese Medicine, 1688 MEILING Avenue, Nanchang, 330004, China
| | - Xiaoyu Su
- Lab of Modern Preparation of TCM, Ministry of Education, Jiangxi University of Chinese Medicine, 1688 MEILING Avenue, Nanchang, 330004, China
| | - Shuiyan Chen
- Lab of Modern Preparation of TCM, Ministry of Education, Jiangxi University of Chinese Medicine, 1688 MEILING Avenue, Nanchang, 330004, China
| | - Xinmin Wang
- Lab of Modern Preparation of TCM, Ministry of Education, Jiangxi University of Chinese Medicine, 1688 MEILING Avenue, Nanchang, 330004, China
| | - Baode Shen
- Lab of Modern Preparation of TCM, Ministry of Education, Jiangxi University of Chinese Medicine, 1688 MEILING Avenue, Nanchang, 330004, China
| | - Hao Song
- Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, Brisbane, QLD, 4072, Australia.
| | - Pengfei Yue
- Lab of Modern Preparation of TCM, Ministry of Education, Jiangxi University of Chinese Medicine, 1688 MEILING Avenue, Nanchang, 330004, China.
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14
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Bhatt S, Pathak R, Punetha VD, Punetha M. Recent advances and mechanism of antimicrobial efficacy of graphene-based materials: a review. JOURNAL OF MATERIALS SCIENCE 2023; 58:7839-7867. [PMID: 37200572 PMCID: PMC10166465 DOI: 10.1007/s10853-023-08534-z] [Citation(s) in RCA: 11] [Impact Index Per Article: 5.5] [Reference Citation Analysis] [Abstract] [Track Full Text] [Figures] [Subscribe] [Scholar Register] [Received: 03/06/2023] [Accepted: 04/24/2023] [Indexed: 05/20/2023]
Abstract
Graphene-based materials have undergone substantial investigation in recent years owing to their wide array of physicochemical characteristics. Employment of these materials in the current state, where infectious illnesses caused by microbes have severely damaged human life, has found widespread application in combating fatal infectious diseases. These materials interact with the physicochemical characteristics of the microbial cell and alter or damage them. The current review is dedicated to molecular mechanisms underlying the antimicrobial property of graphene-based materials. Various physical and chemical mechanisms leading to cell membrane stress, mechanical wrapping, photo-thermal ablation as well as oxidative stress exerting antimicrobial effect have also been thoroughly discussed. Furthermore, an overview of the interactions of these materials with membrane lipids, proteins, and nucleic acids has been provided. A thorough understanding of discussed mechanisms and interactions is essential to develop extremely effective antimicrobial nanomaterial for application as an antimicrobial agent. Graphical abstract
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Affiliation(s)
- Shalini Bhatt
- 2D Materials and LASER Actuation Laboratory, Centre of Excellence for Research, PP Savani University, NH-8, Kosamba-Surat, Gujarat 394125 India
| | - Rakshit Pathak
- 2D Materials and LASER Actuation Laboratory, Centre of Excellence for Research, PP Savani University, NH-8, Kosamba-Surat, Gujarat 394125 India
| | - Vinay Deep Punetha
- 2D Materials and LASER Actuation Laboratory, Centre of Excellence for Research, PP Savani University, NH-8, Kosamba-Surat, Gujarat 394125 India
| | - Mayank Punetha
- 2D Materials and LASER Actuation Laboratory, Centre of Excellence for Research, PP Savani University, NH-8, Kosamba-Surat, Gujarat 394125 India
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15
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Li X, Cong Y, Ovais M, Cardoso MB, Hameed S, Chen R, Chen M, Wang L. Copper-based nanoparticles against microbial infections. WILEY INTERDISCIPLINARY REVIEWS. NANOMEDICINE AND NANOBIOTECHNOLOGY 2023:e1888. [PMID: 37037205 DOI: 10.1002/wnan.1888] [Citation(s) in RCA: 3] [Impact Index Per Article: 1.5] [Reference Citation Analysis] [Abstract] [Key Words] [Grants] [Track Full Text] [Subscribe] [Scholar Register] [Received: 01/16/2023] [Revised: 02/14/2023] [Accepted: 03/13/2023] [Indexed: 04/12/2023]
Abstract
Drug-resistant bacteria and highly infectious viruses are among the major global threats affecting the human health. There is an immediate need for novel strategies to tackle this challenge. Copper-based nanoparticles (CBNPs) have exhibited a broad antimicrobial capacity and are receiving increasing attention in this context. In this review, we describe the functionalization of CBNPs, elucidate their antibacterial and antiviral activity as well as applications, and briefly review their toxicity, biodistribution, and persistence. The limitations of the current study and potential solutions are also shortly discussed. The review will guide the rational design of functional nanomaterials for antimicrobial application. This article is categorized under: Therapeutic Approaches and Drug Discovery > Nanomedicine for Infectious Disease.
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Affiliation(s)
- Xiumin Li
- Department of Chemistry, College of Sciences, Northeastern University, Shenyang, 110819, Liaoning, China
- CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing, 100049, China
| | - Yalin Cong
- CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing, 100049, China
- CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology of China, Beijing, 100190, China
| | - Muhammad Ovais
- CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology of China, Beijing, 100190, China
| | - Mateus Borba Cardoso
- The Soft and Biological Matter Division, Brazilian Synchrotron Light Laboratory, Institute of Chemistry, University of Campinas, CEP 13083-970 Campinas, São Paulo, CP, 6154, Brazil
| | - Saima Hameed
- CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing, 100049, China
| | - Rui Chen
- Institute of Urban Safety and Environmental Science, Beijing Academy of Science and Technology, Beijing, 100083, China
| | - Mingli Chen
- Department of Chemistry, College of Sciences, Northeastern University, Shenyang, 110819, Liaoning, China
| | - Liming Wang
- CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing, 100049, China
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16
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Almeida MC, da Costa PM, Sousa E, Resende DISP. Emerging Target-Directed Approaches for the Treatment and Diagnosis of Microbial Infections. J Med Chem 2023; 66:32-70. [PMID: 36586133 DOI: 10.1021/acs.jmedchem.2c01212] [Citation(s) in RCA: 10] [Impact Index Per Article: 5.0] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 01/01/2023]
Abstract
With the rising levels of drug resistance, developing efficient antimicrobial therapies has become a priority. A promising strategy is the conjugation of antibiotics with relevant moieties that can potentiate their activity by target-directing. The conjugation of siderophores with antibiotics allows them to act as Trojan horses by hijacking the microorganisms' highly developed iron transport systems and using them to carry the antibiotic into the cell. Through the analysis of relevant examples of the past decade, this Perspective aims to reveal the potential of siderophore-antibiotic Trojan horses for the treatment of infections and the role of siderophores in diagnostic techniques. Other conjugated molecules will be the subject of discussion, namely those involving vitamin B12, carbohydrates, and amino acids, as well as conjugated compounds targeting protein degradation and β-lactamase activated prodrugs.
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Affiliation(s)
- Mariana C Almeida
- Laboratório de Química Orgânica e Farmacêutica, Departamento de Ciências Químicas, FFUP - Faculdade de Farmácia, Universidade do Porto, Rua de Jorge de Viterbo Ferreira 228, 4050-313 Porto, Portugal.,CIIMAR- Centro Interdisciplinar de Investigação Marinha e Ambiental, Terminal de Cruzeiros do Porto de Leixões, 4450-208 Matosinhos, Portugal
| | - Paulo M da Costa
- CIIMAR- Centro Interdisciplinar de Investigação Marinha e Ambiental, Terminal de Cruzeiros do Porto de Leixões, 4450-208 Matosinhos, Portugal.,ICBAS - Instituto de Ciências Biomédicas Abel Salazar, Universidade do Porto, Rua de Jorge Viterbo Ferreira 228, 4050-313 Porto, Portugal
| | - Emília Sousa
- Laboratório de Química Orgânica e Farmacêutica, Departamento de Ciências Químicas, FFUP - Faculdade de Farmácia, Universidade do Porto, Rua de Jorge de Viterbo Ferreira 228, 4050-313 Porto, Portugal.,CIIMAR- Centro Interdisciplinar de Investigação Marinha e Ambiental, Terminal de Cruzeiros do Porto de Leixões, 4450-208 Matosinhos, Portugal
| | - Diana I S P Resende
- Laboratório de Química Orgânica e Farmacêutica, Departamento de Ciências Químicas, FFUP - Faculdade de Farmácia, Universidade do Porto, Rua de Jorge de Viterbo Ferreira 228, 4050-313 Porto, Portugal.,CIIMAR- Centro Interdisciplinar de Investigação Marinha e Ambiental, Terminal de Cruzeiros do Porto de Leixões, 4450-208 Matosinhos, Portugal
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17
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Garapati C, HS. Boddu S, Jacob S, Ranch KM, Patel C, Jayachandra Babu R, Tiwari AK, Yasin H. Photodynamic Therapy: A Special Emphasis on Nanocarrier-mediated Delivery of Photosensitizers in Antimicrobial Therapy. ARAB J CHEM 2023. [DOI: 10.1016/j.arabjc.2023.104583] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 01/17/2023] Open
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18
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dos Santos OAL, Pizzorno Backx B, Abumousa RA, Bououdina M. Environmental Implications Associated with the Development of Nanotechnology: From Synthesis to Disposal. NANOMATERIALS (BASEL, SWITZERLAND) 2022; 12:4319. [PMID: 36500947 PMCID: PMC9740896 DOI: 10.3390/nano12234319] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Figures] [Subscribe] [Scholar Register] [Received: 10/24/2022] [Revised: 11/23/2022] [Accepted: 11/28/2022] [Indexed: 06/17/2023]
Abstract
Nanotechnology remains under continuous development. The unique, fascinating, and tunable properties of nanomaterials make them interesting for diverse applications in different fields such as medicine, agriculture, and remediation. However, knowledge about the risks associated with nanomaterials is still poorly known and presents variable results. Furthermore, the interaction of nanomaterials with biological systems and the environment still needs to be clarified. Moreover, some issues such as toxicity, bioaccumulation, and physicochemical transformations are found to be dependent on several factors such as size, capping agent, and shape, making the comparisons even more complex. This review presents a comprehensive discussion about the consequences of the use and development of nanomaterials regarding their potential risks to the environment as well as human and animal health. For this purpose, we reviewed the entire production chain from manufacturing, product development, applications, and even product disposal to raise the important implications at each stage. In addition, we present the recent developments in terms of risk management and the recycling of nanomaterials. Furthermore, the advances and limitations in the legislation and characterization of nanomaterials are also discussed.
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Affiliation(s)
| | - Bianca Pizzorno Backx
- Campus Duque de Caxias, Universidade Federal do Rio de Janeiro, Duque de Caxias 25240-005, Brazil
| | - Rasha A. Abumousa
- Department of Mathematics and Science, Faculty of Humanities and Sciences, Prince Sultan University, Riyadh 11586, Saudi Arabia
| | - Mohamed Bououdina
- Department of Mathematics and Science, Faculty of Humanities and Sciences, Prince Sultan University, Riyadh 11586, Saudi Arabia
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19
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Wen S, Xiong Y, Cai S, Li H, Zhang X, Sun Q, Yang R. Plasmon-enhanced photothermal properties of Au@Ti 3C 2T x nanosheets for antibacterial applications. NANOSCALE 2022; 14:16572-16580. [PMID: 36314771 DOI: 10.1039/d2nr05115j] [Citation(s) in RCA: 2] [Impact Index Per Article: 0.7] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 06/16/2023]
Abstract
Antibiotic-resistant bacterial strains have become an ever-increasing public concern due to their significant threats to health safety. Nanomaterial-based photothermal treatment has shown potential in antibacterial applications, but many nanomaterials exhibited limited photothermal activity that may compromise their antibacterial efficacies. Herein, we report a novel strategy based on efficient photothermal ablation and physical contact over a supported nanostructure by loading Au nanoparticles (NPs) on few-layered Ti3C2Tx nanosheets (NSs) for antibacterial treatment. Ti3C2Tx NSs are delaminated via etching and sonication, and act as a reductant for the in situ reduction of HAuCl4·xH2O, producing "naked" Au NPs without any stabilizers. Meanwhile, by adjusting the Au/Ti ratio, the size and loading of the Au NPs are finely regulated, thereby providing an ideal model of a surface-clean Au@Ti3C2Tx heterostructure for probing the composition-performance relationship. Upon irradiation with visible light, it exhibits synergistically enhanced photothermal conversion efficiency and stability, owing to the localized surface plasmonic resonance effect of Au NP and Au-NS interactions. Moreover, under visible light irradiation for 10 min, the Au@ Ti3C2Tx heterostructure exhibits excellent antibacterial activity for Gram-positive S. aureus and Gram-negative E. coli, and kills over 99% bacteria with a low dose of the nanomedicine suspension (50 μg mL-1). The work demonstrates that the incorporation of transition metal carbides with plasmonic metal nanostructures is an effective strategy to enhance the photothermal antibacterial efficacy.
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Affiliation(s)
- Shiqi Wen
- CAS Key Lab for Biomedical Effects of Nanomaterials and Nanosafety, Center of Materials Science and Optoelectronics Engineering, CAS centre for Excellence in Nanoscience, National Center for Nanoscience and Technology, University of Chinese Academy of Sciences, Beijing 100190, China.
- Sino-Danish College, Sino-Danish Center for Education and Research, University of Chinese Academy of Sciences, Beijing 100190, China
| | - Youlin Xiong
- CAS Key Lab for Biomedical Effects of Nanomaterials and Nanosafety, Center of Materials Science and Optoelectronics Engineering, CAS centre for Excellence in Nanoscience, National Center for Nanoscience and Technology, University of Chinese Academy of Sciences, Beijing 100190, China.
| | - Shuangfei Cai
- CAS Key Lab for Biomedical Effects of Nanomaterials and Nanosafety, Center of Materials Science and Optoelectronics Engineering, CAS centre for Excellence in Nanoscience, National Center for Nanoscience and Technology, University of Chinese Academy of Sciences, Beijing 100190, China.
| | - Haolin Li
- CAS Key Lab for Biomedical Effects of Nanomaterials and Nanosafety, Center of Materials Science and Optoelectronics Engineering, CAS centre for Excellence in Nanoscience, National Center for Nanoscience and Technology, University of Chinese Academy of Sciences, Beijing 100190, China.
- Sino-Danish College, Sino-Danish Center for Education and Research, University of Chinese Academy of Sciences, Beijing 100190, China
| | - Xining Zhang
- CAS Key Lab for Biomedical Effects of Nanomaterials and Nanosafety, Center of Materials Science and Optoelectronics Engineering, CAS centre for Excellence in Nanoscience, National Center for Nanoscience and Technology, University of Chinese Academy of Sciences, Beijing 100190, China.
- Sino-Danish College, Sino-Danish Center for Education and Research, University of Chinese Academy of Sciences, Beijing 100190, China
| | - Qian Sun
- CAS Key Lab for Biomedical Effects of Nanomaterials and Nanosafety, Center of Materials Science and Optoelectronics Engineering, CAS centre for Excellence in Nanoscience, National Center for Nanoscience and Technology, University of Chinese Academy of Sciences, Beijing 100190, China.
| | - Rong Yang
- CAS Key Lab for Biomedical Effects of Nanomaterials and Nanosafety, Center of Materials Science and Optoelectronics Engineering, CAS centre for Excellence in Nanoscience, National Center for Nanoscience and Technology, University of Chinese Academy of Sciences, Beijing 100190, China.
- Sino-Danish College, Sino-Danish Center for Education and Research, University of Chinese Academy of Sciences, Beijing 100190, China
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20
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Sun C, Wang X, Dai J, Ju Y. Metal and Metal Oxide Nanomaterials for Fighting Planktonic Bacteria and Biofilms: A Review Emphasizing on Mechanistic Aspects. Int J Mol Sci 2022; 23:11348. [PMID: 36232647 PMCID: PMC9569886 DOI: 10.3390/ijms231911348] [Citation(s) in RCA: 11] [Impact Index Per Article: 3.7] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Grants] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 08/11/2022] [Revised: 09/14/2022] [Accepted: 09/15/2022] [Indexed: 11/16/2022] Open
Abstract
The misuse and mismanagement of antibiotics have made the treatment of bacterial infections a challenge. This challenge is magnified when bacteria form biofilms, which can increase bacterial resistance up to 1000 times. It is desirable to develop anti-infective materials with antibacterial activity and no resistance to drugs. With the rapid development of nanotechnology, anti-infective strategies based on metal and metal oxide nanomaterials have been widely used in antibacterial and antibiofilm treatments. Here, this review expounds on the state-of-the-art applications of metal and metal oxide nanomaterials in bacterial infective diseases. A specific attention is given to the antibacterial mechanisms of metal and metal oxide nanomaterials, including disrupting cell membranes, damaging proteins, and nucleic acid. Moreover, a practical antibiofilm mechanism employing these metal and metal oxide nanomaterials is also introduced based on the composition of biofilm, including extracellular polymeric substance, quorum sensing, and bacteria. Finally, current challenges and future perspectives of metal and metal oxide nanomaterials in the anti-infective field are presented to facilitate their development and use.
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Affiliation(s)
- Caixia Sun
- College of Pharmacy, China Pharmaceutical University, Nanjing 211198, China
| | - Xiaobai Wang
- Department of Materials Application Research, AVIC Manufacturing Technology Institute, Beijing 100024, China
| | - Jianjun Dai
- College of Pharmacy, China Pharmaceutical University, Nanjing 211198, China
- College of Life Science and Technology, China Pharmaceutical University, Nanjing 211198, China
- Key Laboratory of Drug Quality Control and Pharmacovigilance (Ministry of Education), China Pharmaceutical University, Nanjing 211198, China
- State Key Laboratory of Natural Medicine, China Pharmaceutical University, Nanjing 211198, China
- MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China
- Laboratory of Animal Bacteriology (Ministry of Agriculture), College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China
| | - Yanmin Ju
- College of Pharmacy, China Pharmaceutical University, Nanjing 211198, China
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21
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Prasastha Ram V, Yasur J, Abishad P, Unni V, Purushottam Gourkhede D, Nishanth MAD, Niveditha P, Vergis J, Singh Malik SV, Kullaiah B, Kurkure NV, Ramesh C, Dufossé L, Rawool DB, Barbuddhe SB. Antimicrobial Efficacy of Green Synthesized Nanosilver with Entrapped Cinnamaldehyde against Multi-Drug-Resistant Enteroaggregative Escherichia coli in Galleria mellonella. Pharmaceutics 2022; 14:1924. [PMID: 36145672 PMCID: PMC9503582 DOI: 10.3390/pharmaceutics14091924] [Citation(s) in RCA: 6] [Impact Index Per Article: 2.0] [Reference Citation Analysis] [Abstract] [Key Words] [Grants] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 07/21/2022] [Revised: 09/06/2022] [Accepted: 09/07/2022] [Indexed: 12/11/2022] Open
Abstract
The global emergence of antimicrobial resistance (AMR) needs no emphasis. In this study, the in vitro stability, safety, and antimicrobial efficacy of nanosilver-entrapped cinnamaldehyde (AgC) against multi-drug-resistant (MDR) strains of enteroaggregative Escherichia coli (EAEC) were investigated. Further, the in vivo antibacterial efficacy of AgC against MDR-EAEC was also assessed in Galleria mellonella larval model. In brief, UV-Vis and Fourier transform infrared (FTIR) spectroscopy confirmed effective entrapment of cinnamaldehyde with nanosilver, and the loading efficiency was estimated to be 29.50 ± 0.56%. The AgC was of crystalline form as determined by the X-ray diffractogram with a mono-dispersed spherical morphology of 9.243 ± 1.83 nm in electron microscopy. AgC exhibited a minimum inhibitory concentration (MIC) of 0.008−0.016 mg/mL and a minimum bactericidal concentration (MBC) of 0.008−0.032 mg/mL against MDR- EAEC strains. Furthermore, AgC was stable (high-end temperatures, proteases, cationic salts, pH, and host sera) and tested safe for sheep erythrocytes as well as secondary cell lines (RAW 264.7 and HEp-2) with no negative effects on the commensal gut lactobacilli. in vitro, time-kill assays revealed that MBC levels of AgC could eliminate MDR-EAEC infection in 120 min. In G. mellonella larvae, AgC (MBC values) increased survival, decreased MDR-EAEC counts (p < 0.001), had an enhanced immunomodulatory effect, and was tested safe to the host. These findings infer that entrapment enhanced the efficacy of cinnamaldehyde and AgNPs, overcoming their limitations when used individually, indicating AgC as a promising alternative antimicrobial candidate. However, further investigation in appropriate animal models is required to declare its application against MDR pathogens.
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Affiliation(s)
- Vemula Prasastha Ram
- Division of Veterinary Public Health, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly 243122, India
- ICAR-National Research Centre on Meat, Hyderabad 500092, India
| | - Jyothsna Yasur
- ICAR-National Research Centre on Meat, Hyderabad 500092, India
| | - Padikkamannil Abishad
- Department of Veterinary Public Health, College of Veterinary and Animal Sciences, Pookode, Kerala Veterinary and Animal Sciences University, Pookode 673576, India
| | - Varsha Unni
- Department of Veterinary Public Health, College of Veterinary and Animal Sciences, Pookode, Kerala Veterinary and Animal Sciences University, Pookode 673576, India
| | - Diksha Purushottam Gourkhede
- Division of Veterinary Public Health, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly 243122, India
- ICAR-National Research Centre on Meat, Hyderabad 500092, India
| | - Maria Anto Dani Nishanth
- Division of Veterinary Public Health, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly 243122, India
- ICAR-National Research Centre on Meat, Hyderabad 500092, India
| | | | - Jess Vergis
- Department of Veterinary Public Health, College of Veterinary and Animal Sciences, Pookode, Kerala Veterinary and Animal Sciences University, Pookode 673576, India
| | - Satya Veer Singh Malik
- Division of Veterinary Public Health, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly 243122, India
| | - Byrappa Kullaiah
- Centre for Research and Innovations, BGS Institute of Technology, Adichunchanagiri University, Mandya 571448, India
| | | | - Chatragadda Ramesh
- Biological Oceanography Division (BOD), Council of Scientific and Industrial Research, National Institute of Oceanography (CSIR-NIO), Dona Paula 403004, India
| | - Laurent Dufossé
- Chemistry and Biotechnology of Natural Products (CHEMBIOPRO Lab), Département Agroalimentaire, Ecole Supérieure d’Ingénieurs Réunion Océan Indien (ESIROI), Université de La Réunion, F-97744 Saint-Denis, France
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22
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Manivasagan P, Khan F, Rajan Dhatchayeny D, Park S, Joe A, Han HW, Seo SH, Thambi T, Giang Phan VH, Kim YM, Kim CS, Oh J, Jang ES. Antibody-targeted and streptomycin-chitosan oligosaccharide-modified gold nanoshells for synergistic chemo-photothermal therapy of drug-resistant bacterial infection. J Adv Res 2022:S2090-1232(22)00190-4. [PMID: 36041689 DOI: 10.1016/j.jare.2022.08.009] [Citation(s) in RCA: 4] [Impact Index Per Article: 1.3] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 06/09/2022] [Revised: 08/02/2022] [Accepted: 08/14/2022] [Indexed: 10/15/2022] Open
Abstract
Despite the many advanced strategies that are available, rapid gene mutation in multidrug-resistant bacterial infections remains a major challenge. Combining new therapeutic strategies such as chemo-photothermal therapy (PTT) with high antibacterial efficiency against drug-resistant Listeria monocytogenes (LM) is urgently needed. Here, we report synergistic chemo-PTT against drug-resistant LM based on antibody-targeted and streptomycin-chitosan oligosaccharide-modified gold nanoshells (anti-STR-CO-GNSs) as all-in-one nanotheranostic agents for the first time, which was used for accurate antibacterial applications. The anti-STR-CO-GNSs showed excellent photothermal conversion efficiency (31.97%) and were responsive to near-infrared (NIR) and pH dual stimuli-triggered antibiotic release, resulting in outstanding chemo-photothermal effects against LM. In vitro chemo-photothermal effect of anti-STR-CO-GNSs with laser irradiation caused a greater antibacterial effect (1.37%), resulting in more rapid killing of LM and prevention of LM regrowth. Most importantly, the mice receiving the anti-STR-CO-GNSs with laser irradiation specifically at the sites of LM infections healed almost completely, leaving only scars on the surface of the skin and resulting in superior inhibitory effects from combined chemo-PTT. Overall, our findings suggest that chemo-PTT using smart biocompatible anti-STR-CO-GNSs is a favorable potential alternative to combat the increasing threat of drug-resistant LM, which opens a new door for clinical anti-infection therapy in the future.
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Affiliation(s)
- Panchanathan Manivasagan
- Department of Applied Chemistry, Kumoh National Institute of Technology, Gumi, Gyeongbuk 730-701, Republic of Korea
| | - Fazlurrahman Khan
- Department of Food Science and Technology, Pukyong National University, Busan, 48513, Republic of Korea
| | - Durai Rajan Dhatchayeny
- Department of Information and Communications Engineering, Pukyong National University, Busan, 48513, Republic of Korea
| | - Sumin Park
- Industry 4.0 Convergence Bionics Engineering, Pukyong National University, Busan, 48513, Republic of Korea
| | - Ara Joe
- Department of Applied Chemistry, Kumoh National Institute of Technology, Gumi, Gyeongbuk 730-701, Republic of Korea
| | - Hyo-Won Han
- Department of Applied Chemistry, Kumoh National Institute of Technology, Gumi, Gyeongbuk 730-701, Republic of Korea
| | - Sun-Hwa Seo
- Department of Applied Chemistry, Kumoh National Institute of Technology, Gumi, Gyeongbuk 730-701, Republic of Korea
| | - Thavasyappan Thambi
- School of Chemical Engineering, Theranostic Macromolecules Research Center, Sungkyunkwan University, Suwon 16419, Republic of Korea
| | - V H Giang Phan
- Biomaterials and Nanotechnology Research Group, Faculty of Applied Sciences, Ton Duc Thang University, Ho Chi Minh City 70000, Vietnam
| | - Young-Mog Kim
- Department of Food Science and Technology, Pukyong National University, Busan, 48513, Republic of Korea
| | - Chang-Seok Kim
- Department of Cogno-Mechatronics Engineering, Pusan National University, Busan, 46241, Republic of Korea
| | - Junghwan Oh
- Industry 4.0 Convergence Bionics Engineering, Pukyong National University, Busan, 48513, Republic of Korea; New-senior Healthcare Innovation Center (BK21 Plus), Pukyong National University, Busan, 48513, Republic of Korea; Department of Biomedical Engineering, Pukyong National University, Busan, 48513, Republic of Korea.
| | - Eue-Soon Jang
- Department of Applied Chemistry, Kumoh National Institute of Technology, Gumi, Gyeongbuk 730-701, Republic of Korea.
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23
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Almontasser A, Parveen A. Probing the effect of Ni, Co and Fe doping concentrations on the antibacterial behaviors of MgO nanoparticles. Sci Rep 2022; 12:7922. [PMID: 35562403 PMCID: PMC9106745 DOI: 10.1038/s41598-022-12081-z] [Citation(s) in RCA: 6] [Impact Index Per Article: 2.0] [Reference Citation Analysis] [Abstract] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 12/24/2021] [Accepted: 04/26/2022] [Indexed: 11/09/2022] Open
Abstract
The divalent transition metal ions (Ni, Co, and Fe)-doped MgO nanoparticles were synthesized via the sol–gel method. X-ray diffraction showed the MgO pure, single cubic phase of samples at 600 °C. Field emission electron microscope showed the uniform spherical shape of samples. The magnetic behavior of Ni, Co, Fe-doped MgO system were varied with Ni, Co, Fe content (0.00, 0.01, 0.03, 0.05, 0.07). The magnetic nature of pure had changed from paramagnetic to ferromagnetic. The number of oxygen vacancies increases with increasing amounts of dopant ions that lead to an ionic charge imbalance between Ni2+/Co2+/Fe2+ and Mg2+, leading to increase magnetic properties of the samples. The magnetic nature of prepared samples makes them suitable for biomedical applications. A comparative study of the antibacterial activity of nanoparticles against the Gram-negative (E. coli) and Gram-positive bacteria (S. aureus) was performed by disc diffusion, pour plate techniques, and study surface morphology of untreated and treated bacterial cell wall. An investigation of the antibacterial activity of doped MgO nanoparticles reveals that the doped MgO nanoparticles show effective antibacterial activity against the Gram-negative (E. coli) and Gram-positive (S. aureus) bacterium. The minimum inhibitory concentration of the synthesized nanoparticles against microorganisms was recorded with 40 μg/ml, while the maximum inhibitory concentration was observed with 80 μg/ml. At a concentration of 80 μg/ml, the complete growth inhibition of the E. coli was achieved with 7% Co-doped MgO and 7% Fe-doped MgO, while bacterial growth of S. aureus was inhibited by 100% in the presence of 7% Fe-doped MgO. The present work is promising for using nanomaterials as a novel antibiotic instead of the conventional antibiotics for the treatment of infectious diseases which are caused by tested bacteria.
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Affiliation(s)
- Asma Almontasser
- Department of Applied Physics, Z. H. College of Engineering & Technology, Aligarh Muslim University, Aligarh, 202002, India
| | - Azra Parveen
- Department of Applied Physics, Z. H. College of Engineering & Technology, Aligarh Muslim University, Aligarh, 202002, India.
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24
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Size-dependent photothermal antibacterial activity of Ti 3C 2T x MXene nanosheets against methicillin-resistant Staphylococcus aureus. J Colloid Interface Sci 2022; 617:533-541. [PMID: 35299127 DOI: 10.1016/j.jcis.2022.03.032] [Citation(s) in RCA: 55] [Impact Index Per Article: 18.3] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 01/10/2022] [Revised: 03/06/2022] [Accepted: 03/07/2022] [Indexed: 02/08/2023]
Abstract
Developing antibiotics-independent antibacterial materials is of great importance for combating drug-resistant bacteria, such as methicillin-resistant Staphylococcus aureus (MRSA). MXene (transition metal carbides and nitrides), a class of novel 2D nanomaterials, has shown great potentials in biomedical areas. However, the effect of MXene size on its properties and bioactivity is still unknown. Herein, we report for the first time that the antibacterial photothermal therapy efficacy of Ti3C2Tx MXene nanosheets is size-dependent. Three MXene suspensions with small size of 196 nm (MX-s), medium size of 347 nm (MX-m) and large size of 497 nm (MX-l) were prepared via ultrasonication. Upon NIR irradiation for 5 min, the temperature of MXene suspensions (10 μg/mL) increased to 64, 60 and 56 °C for MX-s, MX-m and MX-l, respectively. Accordingly, the viability loss of MRSA induced by MX-s, MX-m and MX-l under NIR was 93%, 69% and 56%, respectively. The in vivo study in the MRSA-infected mouse model showed that the photothermal therapy efficacy of MX-s was comparable to that of the positive control vancomycin. This is the first report on the size-dependent photothermal effect and photothermal antibacterial activity of MXene, which may guide the development of MXene-based therapeutics in the future. In addition, the drug-free antibacterial therapy has great implications for the treatment of antibiotics-resistant bacteria infections.
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25
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Jin B, Chen JY, Sheng ZL, Sun MQ, Yang HL. Synthesis, Antibacterial and Anthelmintic Activity of Novel 3-(3-Pyridyl)-oxazolidinone-5-methyl Ester Derivatives. Molecules 2022; 27:1103. [PMID: 35164368 PMCID: PMC8839302 DOI: 10.3390/molecules27031103] [Citation(s) in RCA: 6] [Impact Index Per Article: 2.0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 12/25/2021] [Revised: 01/29/2022] [Accepted: 02/03/2022] [Indexed: 11/16/2022] Open
Abstract
In this study, a series of 3-(3-pyridyl)-oxazolidone-5-methyl ester derivatives was synthesized and characterized by 1H NMR, 13C NMR, and LC-MS. The conducted screening antibacterial studies of the new 3-(3-pyridyl)-oxazolidone-5-methyl ester derivatives established that the methyl sulfonic acid esters have broad activity spectrum towards Staphylococcus aureus, Streptococcus pneumoniae, Bacillus subtilis and Staphylococcus epidermidis. Among them, compound 12e has the most potent activity, with an MIC of 16 μg/mL against B.subtilis, and could reduce the instantaneous growth rate of bacteria. Furthermore, molecular docking studies were also simulated for compound 12e to predict the specific binding mode of this compound. In addition, anthelmintic activity of these compounds was also evaluated against adult Indian earthworms (Pheretima posthuman). The results showed that compound 11b had the best effect. These results above can provide experimental reference for the development of novel antibacterial and anthelmintic drugs.
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Affiliation(s)
- Bo Jin
- Department of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China; (B.J.); (J.-y.C.); (Z.-l.S.); (M.-q.S.)
| | - Jia-yi Chen
- Department of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China; (B.J.); (J.-y.C.); (Z.-l.S.); (M.-q.S.)
| | - Zun-lai Sheng
- Department of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China; (B.J.); (J.-y.C.); (Z.-l.S.); (M.-q.S.)
- Heilongjiang Key Laboratory for Animal Disease Control and Pharmaceutical Development, Northeast Agricultural University, Harbin 150030, China
| | - Meng-qing Sun
- Department of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China; (B.J.); (J.-y.C.); (Z.-l.S.); (M.-q.S.)
- Heilongjiang Key Laboratory for Animal Disease Control and Pharmaceutical Development, Northeast Agricultural University, Harbin 150030, China
| | - Hong-liang Yang
- Department of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China; (B.J.); (J.-y.C.); (Z.-l.S.); (M.-q.S.)
- Heilongjiang Key Laboratory for Animal Disease Control and Pharmaceutical Development, Northeast Agricultural University, Harbin 150030, China
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26
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Niu P, Dai J, Wang Z, Wang Y, Feng D, Li Y, Miao W. Sensitization of Antibiotic-Resistant Gram-Negative Bacteria to Photodynamic Therapy via Perfluorocarbon Nanoemulsion. Pharmaceuticals (Basel) 2022; 15:ph15020156. [PMID: 35215269 PMCID: PMC8878207 DOI: 10.3390/ph15020156] [Citation(s) in RCA: 5] [Impact Index Per Article: 1.7] [Reference Citation Analysis] [Abstract] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 12/26/2021] [Revised: 01/24/2022] [Accepted: 01/26/2022] [Indexed: 02/01/2023] Open
Abstract
With the merits of excellent efficacy, safety, and facile implementation, antibacterial photodynamic therapy (APDT) represents a promising means for treating bacterial infections. However, APDT shows an unsatisfactory efficacy in combating antibiotic-resistant Gram-negative bacteria due to their specific cell wall structure. In this work, we report a perfluorocarbon nanoemulsion (Ce6@FDC) used as a multifunctional nanocargo of photosensitizer and oxygen for sensitizing antibiotic-resistant Gram-negative bacteria to APDT. Ce6@FDC was fabricated via ultrasonic emulsification with good colloidal stability, efficient Ce6 and oxygen delivery, and excellent photodynamic activity. Meanwhile, Ce6@FDC could strongly bind with Gram-negative Acinetobacter baumannii (A. baumannii) and Escherichia coli (E. coli) via electrostatic interaction, thus leading to notable photodynamic bactericidal potency upon irradiation. In addition, oxygenated Ce6@FDC also exhibited a remarkable efficacy in eradicating Gram-negative bacteria biofilm, averaging five log units lower than the Ce6 group under identical conditions. Taken together, we demonstrate that photodynamic perfluorocarbon nanoemulsion with oxygen-delivery ability could effectively kill planktonic bacteria and remove biofilm, representing a novel strategy in fighting against antibiotic-resistant Gram-negative bacteria.
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Affiliation(s)
| | | | | | | | | | - Yuanyuan Li
- Correspondence: (Y.L.); (W.M.); Tel.: +86-25-58139399 (W.M.)
| | - Wenjun Miao
- Correspondence: (Y.L.); (W.M.); Tel.: +86-25-58139399 (W.M.)
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27
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Zhao Y, Liu S, Shi Z, Zhu H, Li M, Yu Q. Pathogen infection-responsive nanoplatform targeting macrophage endoplasmic reticulum for treating life-threatening systemic infection. NANO RESEARCH 2022; 15:6243-6255. [PMID: 35382032 PMCID: PMC8972645 DOI: 10.1007/s12274-022-4211-z] [Citation(s) in RCA: 11] [Impact Index Per Article: 3.7] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Subscribe] [Scholar Register] [Received: 12/21/2021] [Revised: 02/01/2022] [Accepted: 02/04/2022] [Indexed: 05/03/2023]
Abstract
UNLABELLED Systemic infections caused by life-threatening pathogens represent one of the main factors leading to clinical death. In this study, we developed a pathogen infection-responsive and macrophage endoplasmic reticulum-targeting nanoplatform to alleviate systemic infections. The nanoplatform is composed of large-pore mesoporous silica nanoparticles (MSNs) grafted by an endoplasmic reticulum-targeting peptide, and a pathogen infection-responsive cap containing the reactive oxygen species-cleavable boronobenzyl acid linker and bovine serum albumin. The capped MSNs exhibited the capacity to high-efficiently load the antimicrobial peptide melittin, and to rapidly release the cargo triggered by H2O2 or the pathogen-macrophage interaction system, but had no obvious toxicity to macrophages. During the interaction with pathogenic Candida albicans cells and macrophages, the melittin-loading nanoplatform MSNE+MEL+TPB strongly inhibited pathogen growth, survived macrophages, and suppressed endoplasmic reticulum stress together with pro-inflammatory cytokine secretion. In a systemic infection model, the nanoplatform efficiently prevented kidney dysfunction, alleviated inflammatory symptoms, and protected the mice from death. This study developed a macrophage organelle-targeting nanoplatform for treatment of life-threatening systemic infections. ELECTRONIC SUPPLEMENTARY MATERIAL Supplementary material (N2 adsorption curves of the initial synthesized MSNs, FT-IR spectra of MSN, and MSNE, MEL release from the FITC-MEL-loading MSNE + TPB induced by different concentration of H2O2, viability of NIH3T3 cells, and DC2.4 cells after treatment of free MEL or the used nanoparticles, effect of MEL on C. albicans growth and macrophage death during the interaction between C. albicans and macrophages, effect of MEL and the nanoparticles on S. aureus growth and macrophage death during the interaction between S. aureus and macrophages, quantification of GRP78 (a) and activated Caspase-3, flow cytometry analysis of kidney non-macrophages with the Alexa Fluor 594 signal, survival curve of the infected mice treated by MEL or MSNE + MEL, kidney burden, blood urea levels and serum TNF-α levels in the infected mice) is available in the online version of this article at 10.1007/s12274-022-4211-z.
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Affiliation(s)
- Yan Zhao
- Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Department of Microbiology, College of Life Sciences, Nankai University, Tianjin, 300071 China
| | - Shuo Liu
- Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Department of Microbiology, College of Life Sciences, Nankai University, Tianjin, 300071 China
- College of Environmental Science and Engineering, Tianjin Key Laboratory of Environmental Remediation and Pollution Control, Nankai University, Tianjin, 300350 China
| | - Zhishang Shi
- Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Department of Microbiology, College of Life Sciences, Nankai University, Tianjin, 300071 China
| | - Hangqi Zhu
- Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Department of Microbiology, College of Life Sciences, Nankai University, Tianjin, 300071 China
| | - Mingchun Li
- Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Department of Microbiology, College of Life Sciences, Nankai University, Tianjin, 300071 China
| | - Qilin Yu
- Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Department of Microbiology, College of Life Sciences, Nankai University, Tianjin, 300071 China
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28
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Zhang H, Xu Z, Mao Y, Zhang Y, Li Y, Lao J, Wang L. Integrating Porphyrinic Metal-Organic Frameworks in Nanofibrous Carrier for Photodynamic Antimicrobial Application. Polymers (Basel) 2021; 13:polym13223942. [PMID: 34833240 PMCID: PMC8625335 DOI: 10.3390/polym13223942] [Citation(s) in RCA: 7] [Impact Index Per Article: 1.8] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 10/13/2021] [Revised: 11/08/2021] [Accepted: 11/10/2021] [Indexed: 01/21/2023] Open
Abstract
The rise and spread of antimicrobial resistance is creating an ever greater challenge in wound management. Nanofibrous membranes (NFMs) incorporated with antibiotics have been widely used to remedy bacterial wound infections owing to their versatile features. However, misuse of antibiotics has resulted in drug resistance, and it remains a significant challenge to achieve both high antibacterial efficiency and without causing bacterial resistance. Here, the ‘MOF-first’ strategy was adopted, the porphyrinic metal-organic frameworks nanoparticles (PCN−224 NPs) were pre-synthesized first, and then the composite antibacterial PCN−224 NPs @ poly (ε-caprolactone) (PM) NFMs were fabricated via a facile co-electrospinning technology. This strategy allows large amounts of effective MOFs to be integrated into nanofibers to effectively eliminate bacteria without bacterial resistance and to realize a relatively fast production rate. Upon visible light (630 nm) irradiation for 30 min, the PM−25 NFMs have the best 1O2 generation performance, triggering remarkable photodynamic antibacterial effects against both S. aureus, MRSA, and E. coli bacteria with survival rates of 0.13%, 1.91%, and 2.06% respectively. Considering the photodynamic antibacterial performance of the composite nanofibrous membranes functionalized by porphyrinic MOFs, this simple approach may provide a feasible way to use MOF materials and biological materials to construct wound dressing with the versatility to serve as an antibacterial strategy in order to prevent bacterial resistance.
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Affiliation(s)
- Huiru Zhang
- Key Laboratory of Textile Science & Technology, Ministry of Education, College of Textiles, Donghua University, Shanghai 201620, China; (H.Z.); (Z.X.); (Y.M.); (Y.Z.); (J.L.); (L.W.)
- Key Laboratory of Textile Industry for Biomedical Textile Materials and Technology, Donghua University, Shanghai 201620, China
| | - Zhihao Xu
- Key Laboratory of Textile Science & Technology, Ministry of Education, College of Textiles, Donghua University, Shanghai 201620, China; (H.Z.); (Z.X.); (Y.M.); (Y.Z.); (J.L.); (L.W.)
- Key Laboratory of Textile Industry for Biomedical Textile Materials and Technology, Donghua University, Shanghai 201620, China
| | - Ying Mao
- Key Laboratory of Textile Science & Technology, Ministry of Education, College of Textiles, Donghua University, Shanghai 201620, China; (H.Z.); (Z.X.); (Y.M.); (Y.Z.); (J.L.); (L.W.)
- Key Laboratory of Textile Industry for Biomedical Textile Materials and Technology, Donghua University, Shanghai 201620, China
| | - Yingjie Zhang
- Key Laboratory of Textile Science & Technology, Ministry of Education, College of Textiles, Donghua University, Shanghai 201620, China; (H.Z.); (Z.X.); (Y.M.); (Y.Z.); (J.L.); (L.W.)
- Key Laboratory of Textile Industry for Biomedical Textile Materials and Technology, Donghua University, Shanghai 201620, China
| | - Yan Li
- Key Laboratory of Textile Science & Technology, Ministry of Education, College of Textiles, Donghua University, Shanghai 201620, China; (H.Z.); (Z.X.); (Y.M.); (Y.Z.); (J.L.); (L.W.)
- Key Laboratory of Textile Industry for Biomedical Textile Materials and Technology, Donghua University, Shanghai 201620, China
- Correspondence: ; Tel.: +86-21-6779-2634
| | - Jihong Lao
- Key Laboratory of Textile Science & Technology, Ministry of Education, College of Textiles, Donghua University, Shanghai 201620, China; (H.Z.); (Z.X.); (Y.M.); (Y.Z.); (J.L.); (L.W.)
- Key Laboratory of Textile Industry for Biomedical Textile Materials and Technology, Donghua University, Shanghai 201620, China
| | - Lu Wang
- Key Laboratory of Textile Science & Technology, Ministry of Education, College of Textiles, Donghua University, Shanghai 201620, China; (H.Z.); (Z.X.); (Y.M.); (Y.Z.); (J.L.); (L.W.)
- Key Laboratory of Textile Industry for Biomedical Textile Materials and Technology, Donghua University, Shanghai 201620, China
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29
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Kaur K, Reddy S, Barathe P, Shriram V, Anand U, Proćków J, Kumar V. Combating Drug-Resistant Bacteria Using Photothermally Active Nanomaterials: A Perspective Review. Front Microbiol 2021; 12:747019. [PMID: 34867863 PMCID: PMC8633304 DOI: 10.3389/fmicb.2021.747019] [Citation(s) in RCA: 31] [Impact Index Per Article: 7.8] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 07/25/2021] [Accepted: 10/15/2021] [Indexed: 01/15/2023] Open
Abstract
Injudicious use of antibiotics has been the main driver of severe bacterial non-susceptibility to commonly available antibiotics (known as drug resistance or antimicrobial resistance), a global threat to human health and healthcare. There is an increase in the incidence and levels of resistance to antibacterial drugs not only in nosocomial settings but also in community ones. The drying pipeline of new and effective antibiotics has further worsened the situation and is leading to a potentially "post-antibiotic era." This requires novel and effective therapies and therapeutic agents for combating drug-resistant pathogenic microbes. Nanomaterials are emerging as potent antimicrobial agents with both bactericidal and potentiating effects reported against drug-resistant microbes. Among them, the photothermally active nanomaterials (PANs) are gaining attention for their broad-spectrum antibacterial potencies driven mainly by the photothermal effect, which is characterized by the conversion of absorbed photon energy into heat energy by the PANs. The current review capitalizes on the importance of using PANs as an effective approach for overcoming bacterial resistance to drugs. Various PANs leveraging broad-spectrum therapeutic antibacterial (both bactericidal and synergistic) potentials against drug-resistant pathogens have been discussed. The review also provides deeper mechanistic insights into the mechanisms of the action of PANs against a variety of drug-resistant pathogens with a critical evaluation of efflux pumps, cell membrane permeability, biofilm, and quorum sensing inhibition. We also discuss the use of PANs as drug carriers. This review also discusses possible cytotoxicities related to the therapeutic use of PANs and effective strategies to overcome this. Recent developments, success stories, challenges, and prospects are also presented.
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Affiliation(s)
- Kawaljeet Kaur
- Department of Biotechnology, Modern College of Arts, Science and Commerce, Ganeshkhind, Savitribai Phule Pune University, Pune, India
| | - Sagar Reddy
- Department of Botany, Prof. Ramkrishna More College, Savitribai Phule Pune University, Pune, India
| | - Pramod Barathe
- Department of Biotechnology, Modern College of Arts, Science and Commerce, Ganeshkhind, Savitribai Phule Pune University, Pune, India
| | - Varsha Shriram
- Department of Botany, Prof. Ramkrishna More College, Savitribai Phule Pune University, Pune, India
| | - Uttpal Anand
- Department of Life Sciences, Ben-Gurion University of the Negev, Beer Sheva, Israel
| | - Jarosław Proćków
- Department of Plant Biology, Institute of Environmental Biology, Wrocław University of Environmental and Life Sciences, Wrocław, Poland
| | - Vinay Kumar
- Department of Biotechnology, Modern College of Arts, Science and Commerce, Ganeshkhind, Savitribai Phule Pune University, Pune, India
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Bertani R, Bartolozzi A, Pontefisso A, Quaresimin M, Zappalorto M. Improving the Antimicrobial and Mechanical Properties of Epoxy Resins via Nanomodification: An Overview. Molecules 2021; 26:5426. [PMID: 34500859 PMCID: PMC8434237 DOI: 10.3390/molecules26175426] [Citation(s) in RCA: 8] [Impact Index Per Article: 2.0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 08/03/2021] [Accepted: 08/21/2021] [Indexed: 12/25/2022] Open
Abstract
The main purpose of this work is to provide a comprehensive overview on the preparation of multifunctional epoxies, with improved antimicrobial activity and enhanced mechanical properties through nanomodification. In the first section, we focus on the approaches to achieve antimicrobial activity, as well as on the methods used to evaluate their efficacy against bacteria and fungi. Relevant application examples are also discussed, with particular reference to antifouling and anticorrosion coatings for marine environments, dental applications, antimicrobial fibers and fabrics, and others. Subsequently, we discuss the mechanical behaviors of nanomodified epoxies with improved antimicrobial properties, analyzing the typical damage mechanisms leading to the significant toughening effect of nanomodification. Some examples of mechanical properties of nanomodified polymers are provided. Eventually, the possibility of achieving, at the same time, antimicrobial and mechanical improvement capabilities by nanomodification with nanoclay is discussed, with reference to both nanomodified epoxies and glass/epoxy composite laminates. According to the literature, a nanomodified epoxy can successfully exhibit antibacterial properties, while increasing its fracture toughness, even though its tensile strength may decrease. As for laminates-obtaining antibacterial properties is not followed by improved interlaminar properties.
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Affiliation(s)
- Roberta Bertani
- Department of Industrial Engineering, University of Padova, Via F. Marzolo 9, 35131 Padova, Italy;
| | - Alessandra Bartolozzi
- Department of Industrial Engineering, University of Padova, Via F. Marzolo 9, 35131 Padova, Italy;
| | - Alessandro Pontefisso
- Department of Management and Engineering, University of Padova, stradella S. Nicola 3, 36100 Vicenza, Italy; (A.P.); (M.Q.)
| | - Marino Quaresimin
- Department of Management and Engineering, University of Padova, stradella S. Nicola 3, 36100 Vicenza, Italy; (A.P.); (M.Q.)
| | - Michele Zappalorto
- Department of Management and Engineering, University of Padova, stradella S. Nicola 3, 36100 Vicenza, Italy; (A.P.); (M.Q.)
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