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VIJAYARANI K, SUDHA S, ABIRAMI B, PRIYADHARSHINI CV, KUMANAN K. Immunogenicity of chitosan coupled heat killed and fusion protein vaccine of Mycobacterium avium subsp. paratuberculosis. THE INDIAN JOURNAL OF ANIMAL SCIENCES 2021. [DOI: 10.56093/ijans.v91i6.115446] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 11/06/2022]
Abstract
In search for better vaccine candidates for Johne's disease, chitosan nanoparticle coupled heat killed whole cell and fusion protein (Antigen 85A+85B) vaccines were developed using a local isolate of Mycobacterium avium subsp. paratuberculosis. Immunization studies in goats indicated that the heat killed whole cell vaccine and the fusion protein vaccine were found to be safe and resulted in Th1 and Th2 responses. Antibody response was significantly higher in the heat killed whole cell immunized animals than those received the fusion protein. Significant increase in heat killed whole cell MAP and fusion protein specific IFN-γ gene expression was detected in the immunized animals in contrast to the controls. The response was significantly higher with the heat killed whole cell MAP than the fusion protein. The adjuvant effect of chitosan nanoparticles was clearly evident in the second trial wherein the animals were observed for a longer period of 24 weeks. With increased IFN-γ and antibody levels, vaccinated animals responded well as compared to the control ones and the response was better in heat killed whole cell vaccine.
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Campbell Z, Coleman P, Guest A, Kushwaha P, Ramuthivheli T, Osebe T, Perry B, Salt J. Prioritizing smallholder animal health needs in East Africa, West Africa, and South Asia using three approaches: Literature review, expert workshops, and practitioner surveys. Prev Vet Med 2021; 189:105279. [PMID: 33581421 PMCID: PMC8024747 DOI: 10.1016/j.prevetmed.2021.105279] [Citation(s) in RCA: 11] [Impact Index Per Article: 2.8] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 09/17/2020] [Revised: 12/18/2020] [Accepted: 01/18/2021] [Indexed: 12/15/2022]
Abstract
Managing the health needs of livestock contributes to reducing poverty and improving the livelihoods of smallholder and pastoralist livestock keepers globally. Animal health practitioners, producers, policymakers, and researchers all must prioritize how to mobilize limited resources. This study employed three approaches to prioritize animal health needs in East and West Africa and South Asia to identify diseases and syndromes that impact livestock keepers. The approaches were a) systematic literature review, b) a series of expert workshops, and c) a practitioner survey of veterinarians and para-veterinary professionals. The top constraints that emerged from all three approaches include endo/ ectoparasites, foot and mouth disease, brucellosis, peste des petits ruminants, Newcastle disease, and avian influenza. Expert workshops additionally identified contagious caprine pleuropneumonia, contagious bovine pleuropneumonia, mastitis, and reproductive disorders as constraints not emphasized in the literature review. Practitioner survey results additionally identified nutrition as a constraint for smallholder dairy and pastoralist small ruminant production. Experts attending the workshops agreed most constraints can be managed using existing veterinary technologies and best husbandry practices, which supports a shift away from focusing on individual diseases and new technologies towards addressing systemic challenges that limit access to veterinary services and inputs. Few research studies focused on incidence/ prevalence of disease and impact, suggesting better incorporation of socio-economic impact measures in future research would better represent the interests of livestock keepers.
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Affiliation(s)
- Zoë Campbell
- International Livestock Research Institute (ILRI), P.O. Box 30709, Nairobi, 00100, Kenya.
| | - Paul Coleman
- H20 Venture Partners, 33-35 George Street, Oxford, OX1 2AY, United Kingdom
| | - Andrea Guest
- H20 Venture Partners, 33-35 George Street, Oxford, OX1 2AY, United Kingdom
| | - Peetambar Kushwaha
- GALVmed Asia Office, Unit 118 & 120 B, Splendor Forum, Plot No 3, Jasola District Centre, Jasola, New Delhi, 110025, India
| | - Thembinkosi Ramuthivheli
- GALVmed Africa Office, International Livestock Research Institute (ILRI), Swing One, Naivasha Road, Nairobi, Kenya
| | - Tom Osebe
- GALVmed Africa Office, International Livestock Research Institute (ILRI), Swing One, Naivasha Road, Nairobi, Kenya
| | - Brian Perry
- Nuffield College of Clinical Medicine, University of Oxford, United Kingdom; College of Medicine and Veterinary Medicine, University of Edinburgh, Arthurstone House, Meigle, Blairgowrie, PH12 8QW, Scotland, United Kingdom
| | - Jeremy Salt
- GALVmed UK Office, Doherty Building, Pentlands Science Park, Bush Loan, Penicuik Edinburgh, EH26 0PZ, Scotland, United Kingdom
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Gupta S, Singh SV, Singh M, Chaubey KK, Karthik K, Bhatia AK, Kumar N, Dhama K. Vaccine approaches for the 'therapeutic management' of Mycobacterium avium subspecies paratuberculosis infection in domestic livestock. Vet Q 2020; 39:143-152. [PMID: 31524561 PMCID: PMC6831026 DOI: 10.1080/01652176.2019.1667042] [Citation(s) in RCA: 7] [Impact Index Per Article: 1.4] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 02/06/2023] Open
Abstract
High endemicity of Johne’s disease (JD) in herds adversely affects heavy milk yielding breeds by reducing the per animal productivity and ‘productive life-span’. This review evaluates different vaccines used for its control and summarizes the benefits of ‘global vaccine’ in the four major domestic livestock species, namely goat, sheep, buffalo and cattle. Vaccines developed by using ‘native strains’ revealed both 'therapeutic' and preventive effects in domestic livestock. The 'therapeutic' role of vaccine in animals suffering from clinical JD turned out to be valuable in some cases by reversing the disease process and animals returning back to health and production. Good herd management, improved hygiene, ‘test and cull’ methodology, proper disposal of animal excreta and monitoring of MAP bio-load were also regarded as crucial in the 'therapeutic' management of JD. Vaccine approaches have been widely adopted in JD control programs and may be considered as a valuable adjunct in order to utilize huge populations of otherwise un-productive livestock. It has been shown that vaccination was the preeminent strategy to control JD, because it yielded approximately 3–4 times better benefit-to-cost ratios than other strategies. Internationally, 146 vaccine trials/studies have been conducted in different countries for the control of JD and have shown remarkable reduction in its national prevalence. It is concluded that for JD, there cannot be global vaccines or diagnostic kits as solutions have to come from locally prevalent strains of MAP. Despite some limitations, vaccines might still be an effective strategy to reduce or eradicate JD.
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Affiliation(s)
- Saurabh Gupta
- Department of Biotechnology, GLA University , Mathura , Uttar Pradesh , India
| | - Shoor Vir Singh
- Department of Biotechnology, GLA University , Mathura , Uttar Pradesh , India
| | - Manju Singh
- Department of Biotechnology, GLA University , Mathura , Uttar Pradesh , India
| | | | - Kumaragurubaran Karthik
- Central University Laboratory, Tamil Nadu Veterinary and Animal Sciences University , Chennai , Tamil Nadu , India
| | - A K Bhatia
- Department of Biotechnology, GLA University , Mathura , Uttar Pradesh , India
| | - Naveen Kumar
- Veterinary Type Culture Collection, NRC on Equines, Indian Council of Agricultural Research , Hisar , Haryana, India
| | - Kuldeep Dhama
- Division of Pathology, ICAR-Indian Veterinary Research Institute , Izatnagar, Bareilly , Uttar Pradesh , India
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A peptide-based vaccine for Mycobacterium avium subspecies paratuberculosis. Vaccine 2019; 37:2783-2790. [PMID: 31003915 DOI: 10.1016/j.vaccine.2019.04.040] [Citation(s) in RCA: 12] [Impact Index Per Article: 2.0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 08/15/2018] [Revised: 03/26/2019] [Accepted: 04/14/2019] [Indexed: 12/22/2022]
Abstract
Recent efforts to develop a live attenuated vaccine against Mycobacterium avium subsp. paratuberculosis (Map), the causative agent of Johne's disease (JD), revealed relA is important in Map virulence. Deletion of the relA gene impairs the ability of Map to establish a persistent infection. Analysis of the basis for this observation revealed infection with a relA deletion mutant (ΔrelA) elicits development of cytotoxic CD8 T cells (CTL) with the ability to kill intracellular bacteria. Further analysis of the recall response elicited by ΔrelA vaccination showed a 35 kDa membrane peptide (MMP) is one of the targets of the immune response, suggesting it might be possible to develop a peptide-based vaccine based on MMP. To explore this possibility, ex vivo vaccination studies were conducted with MMP alone and incorporated into a nanoparticle (NP) vector comprised of poly (D, L-lactide-co-glycolide) and monophosphoryl lipid A (PLGA/MPLA). As reported, ex vivo vaccination studies showed CD8 CTL were elicited with classic and monocyte derived dendritic cells (cDC and MoDC) pulsed with MMP alone and incorporated into a PGLA/MPLA vector. Incorporation of MMP into a NP vector enhanced the ability of CD8 CTL to kill intracellular bacteria. The findings indicate incorporation of MMP into a PGLA/MPLA nanoparticle vector is one of the possible ways to develop a MMP based vaccine for Johne's disease.
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Barkema HW, Orsel K, Nielsen SS, Koets AP, Rutten VPMG, Bannantine JP, Keefe GP, Kelton DF, Wells SJ, Whittington RJ, Mackintosh CG, Manning EJ, Weber MF, Heuer C, Forde TL, Ritter C, Roche S, Corbett CS, Wolf R, Griebel PJ, Kastelic JP, De Buck J. Knowledge gaps that hamper prevention and control of Mycobacterium avium subspecies paratuberculosis infection. Transbound Emerg Dis 2017; 65 Suppl 1:125-148. [PMID: 28941207 DOI: 10.1111/tbed.12723] [Citation(s) in RCA: 84] [Impact Index Per Article: 10.5] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 05/09/2017] [Indexed: 12/17/2022]
Abstract
In the last decades, many regional and country-wide control programmes for Johne's disease (JD) were developed due to associated economic losses, or because of a possible association with Crohn's disease. These control programmes were often not successful, partly because management protocols were not followed, including the introduction of infected replacement cattle, because tests to identify infected animals were unreliable, and uptake by farmers was not high enough because of a perceived low return on investment. In the absence of a cure or effective commercial vaccines, control of JD is currently primarily based on herd management strategies to avoid infection of cattle and restrict within-farm and farm-to-farm transmission. Although JD control programmes have been implemented in most developed countries, lessons learned from JD prevention and control programmes are underreported. Also, JD control programmes are typically evaluated in a limited number of herds and the duration of the study is less than 5 year, making it difficult to adequately assess the efficacy of control programmes. In this manuscript, we identify the most important gaps in knowledge hampering JD prevention and control programmes, including vaccination and diagnostics. Secondly, we discuss directions that research should take to address those knowledge gaps.
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Affiliation(s)
- H W Barkema
- Department of Production Animal Health, Faculty of Veterinary Medicine, University of Calgary, Calgary, AB, Canada
| | - K Orsel
- Department of Production Animal Health, Faculty of Veterinary Medicine, University of Calgary, Calgary, AB, Canada
| | - S S Nielsen
- University of Copenhagen, Copenhagen, Denmark
| | - A P Koets
- Utrecht University, Utrecht, The Netherlands.,Wageningen Bioveterinary Research, Wageningen, The Netherlands
| | - V P M G Rutten
- Utrecht University, Utrecht, The Netherlands.,Department of Veterinary Tropical Diseases, Faculty of Veterinary Science, University of Pretoria, Private Bag X04, Onderstepoort, 0110, South Africa
| | | | - G P Keefe
- University of Prince Edward Island, Charlottetown, Canada
| | | | - S J Wells
- University of Minnesota, Minneapolis, MN, USA
| | | | | | | | - M F Weber
- GD Animal Health, Deventer, The Netherlands
| | - C Heuer
- Massey University, Palmerston North, New Zealand
| | | | - C Ritter
- Department of Production Animal Health, Faculty of Veterinary Medicine, University of Calgary, Calgary, AB, Canada
| | - S Roche
- University of Guelph, Guelph, Canada
| | - C S Corbett
- Department of Production Animal Health, Faculty of Veterinary Medicine, University of Calgary, Calgary, AB, Canada
| | - R Wolf
- Amt der Steiermärkischen Landesregierung, Graz, Austria
| | | | - J P Kastelic
- Department of Production Animal Health, Faculty of Veterinary Medicine, University of Calgary, Calgary, AB, Canada
| | - J De Buck
- Department of Production Animal Health, Faculty of Veterinary Medicine, University of Calgary, Calgary, AB, Canada
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Vir Singh S, Gupta S, Kumar Chau K, Bhushan S, Dutta Rawa K, Kumar N, Audh Tiwar H, Chaturvedi V, Singh Soha J, Dhama K, Hemati Z. ‘Therapeutic Management’ of Incurable Paratuberculosis Using ‘Indigenous Vaccine’ in Goatherds, Endemically Infected with Johne’s Disease. INT J PHARMACOL 2017. [DOI: 10.3923/ijp.2017.145.155] [Citation(s) in RCA: 3] [Impact Index Per Article: 0.4] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/15/2022]
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Arrazuria R, Molina E, Garrido JM, Pérez V, Juste RA, Elguezabal N. Vaccination sequence effects on immunological response and tissue bacterial burden in paratuberculosis infection in a rabbit model. Vet Res 2016; 47:77. [PMID: 27496043 PMCID: PMC4975891 DOI: 10.1186/s13567-016-0360-y] [Citation(s) in RCA: 6] [Impact Index Per Article: 0.7] [Reference Citation Analysis] [Abstract] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 02/24/2016] [Accepted: 06/06/2016] [Indexed: 01/12/2023] Open
Abstract
Paratuberculosis (PTB), a chronic granulomatous enteritis produced by Mycobacterium avium subspecies paratuberculosis (MAP), is considered as one of the diseases with the highest economic impact in the ruminant industry. Vaccination against MAP is recommended during the first months after birth on the basis that protection would be conferred before the first contact with mycobacteria. However, little is known about the therapeutic effect of MAP vaccination in controlled experimental conditions. The current study was designed to evaluate the efficacy of vaccination before and after challenge with MAP in a rabbit infection model. The rabbits were divided into four groups: non-infected control (NIC, n = 4), infected control challenged with MAP (IC, n = 5), vaccinated and challenged 1 month after with MAP (VSI, n = 5) and challenged with MAP and vaccinated 2 months later (IVS, n = 5). The results from this study show a quick increase in IFN-γ release upon stimulation with bovine, avian and johnin PPD in animals vaccinated before MAP challenge. All vaccinated animals show an increased humoral response as seen by western blot and ELISA. The final bacteriology index (considering tissue culture and qPCR) shows that the IC group was the most affected. Vaccination after infection (IVS) produced the lowest bacteriology index showing significant differences with the IC group (p = 0.034). In conclusion, vaccination against MAP shows positive effects in a rabbit model. However, vaccination after infection shows a slightly stronger protective effect compared to vaccination before infection, suggesting a therapeutic effect. This feature could be applied to previously infected adult animals under field conditions.
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Affiliation(s)
- Rakel Arrazuria
- Animal Health Department, NEIKER-Instituto Vasco de Investigación y Desarrollo Agrario, Berreaga, 1, 48160, Derio, Bizkaia, Spain
| | - Elena Molina
- Animal Health Department, NEIKER-Instituto Vasco de Investigación y Desarrollo Agrario, Berreaga, 1, 48160, Derio, Bizkaia, Spain
| | - Joseba M Garrido
- Animal Health Department, NEIKER-Instituto Vasco de Investigación y Desarrollo Agrario, Berreaga, 1, 48160, Derio, Bizkaia, Spain
| | - Valentín Pérez
- Department of Animal Health, Faculty of Veterinary Medicine, University of León, León, Spain
| | - Ramón A Juste
- Animal Health Department, NEIKER-Instituto Vasco de Investigación y Desarrollo Agrario, Berreaga, 1, 48160, Derio, Bizkaia, Spain.,Department of Agriculture of the Regional Government of the Principality of Asturias, SERIDA, Deva, Asturias, Spain
| | - Natalia Elguezabal
- Animal Health Department, NEIKER-Instituto Vasco de Investigación y Desarrollo Agrario, Berreaga, 1, 48160, Derio, Bizkaia, Spain.
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Davis WC. On deaf ears, Mycobacterium avium paratuberculosis in pathogenesis Crohn’s and other diseases. World J Gastroenterol 2015; 21:13411-13417. [PMID: 26730151 PMCID: PMC4690169 DOI: 10.3748/wjg.v21.i48.13411] [Citation(s) in RCA: 22] [Impact Index Per Article: 2.2] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Download PDF] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Received: 08/10/2015] [Revised: 10/22/2015] [Accepted: 11/19/2015] [Indexed: 02/06/2023] Open
Abstract
The historic suggestion that Mycobacterium avium subsp. paratuberculosis (Map) might be a zoonotic pathogen was based on the apparent similarity of lesions in the intestine of patients with Crohn’s disease (CD) with those present in cattle infected with Map, the etiological agent of Johne’s disease. Reluctance to fully explore this possibility has been attributed to the difficulty in demonstrating the presence of Map in tissues from patients with CD. Advances in technology have resolved this problem and revealed the presence of Map in a significant proportion of patients with CD and other diseases. The seminal finding from recent investigations, however, is the detection of Map in healthy individuals with no clinical signs of disease. The latter observation indicates all humans are susceptible to infection with Map and lends support to the thesis that Map is zoonotic, with a latent stage of infection similar to tuberculosis, where infection leads to the development of an immune response that controls but does not eliminate the pathogen. This clarifies one of the reasons why it has been so difficult to document that Map is zoonotic and associated with the pathogenesis of CD and other diseases. As discussed in the present review, a better understanding of the immune response to Map is needed to determine how infection is usually kept under immune control during the latent stage of infection and elucidate the triggering events that lead to disease progression in the natural host and pathogenesis of CD and immune related diseases in humans.
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