Formalin fixation on HER-2 and PD-L1 expression in gastric cancer: A pilot analysis using the same surgical specimens with different fixation times

Table 2 Whole data of immunohistochemistry according to fixation time

Case No.	Formalin	HER-2 IHC score; P = 0.7713 (6 h-48 h vs 1 wk)1				PD-L1 expression (%); P = 0.46 (6 h-48 h vs 1 wk)1
		6 h	24 h	48 h	1 wk	6 h	24 h	48 h	1 wk
1	10% F	0	0	0	0	0	0	0	0
2	10% F	0	0	0	0	10	10	10	1-9
3	10% F	0	0	0	0	0	0	0	0
4	10% F	0	0	0	0	80	80	90	90
5	10% F	0	0	0	0	0	0	0	1-9
6	10% F	0	0	0	0	0	0	0	0
7	10% F	0	0	0	0	0	0	0	0
8	10% F	0	0	0	0	0	0	1-9	1-9
9	10% F	0	0	0	0	0	1-9	0	1-9
10	10% F	0	0	0	0	40	30	30	40
11	10% F	0	0	0	0	1-9	0	10	10
12	10% F	3+	3+	3+	3+	1-9	1-9	1-9	0
13	10% F	0	0	0	0	0	0	0	0
14	10% F	1+	2+	0	0	100	1-9	30	60
15	10% F	0	0	0	0	0	0	0	0
16	10% F	0	0	0	0	1-9	10	1-9	1-9
17	10% F	0	0	0	0	0	0	0	0
18	10% F	0	0	0	0	20	60	20	40
19	10% F	0	0	0	0	1-9	1-9	1-9	0
20	10% F	2+	0	0	0	80	80	20	1-9
21	10% F	0	0	0	0	1-9	0	1-9	1-9
22	10% F	1+	1+	2+	2+	0	0	0	0
23	10% NBF	0	0	0	0	0	0	0	0
24	10% NBF	0	0	0	0	0	0	0	0
25	10% NBF	0	1+	2+	1+	10	0	10	0
26	10% NBF	2+	2+	2+	2+	0	0	0	0
27	10% NBF	2+	1+	1+	3+	20	70	30	1-9
28	10% NBF	0	0	0	0	0	0	0	0
29	10% NBF	0	0	0	0	1-9	1-9	0	1-9
30	10% NBF	0	0	0	0	100	100	100	100
31	10% NBF	3+	3+	3+	3+	0	1-9	10	1-9
32	10% NBF	0	0	0	0	70	80	90	90

¹Simultaneous tests for linear hypotheses based on a linear mixed effects model.

F: Formalin; NBF: Neutral buffered formalin; HER-2: Human epidermal growth factor receptor 2; PD-L1: Programmed death-ligand 1.