Circulating miR-627-5p and miR-199a-5p are promising diagnostic biomarkers of colorectal neoplasia

Figure 7 MiR-627-5p and miR-199a-5p acted as tumor suppressors in SW480 cells. A: The increased levels of miR-627-5p and miR-199a-5p in SW480 cells were confirmed by quantitative real-time polymerase chain reaction after transfection with specifically synthesized mimics; B: Cell counting kit-8 assay showed that miR-627-5p and miR-199a-5p mimics inhibited the viability of SW480 cells compared to the control group; C: Wound healing assay showed that miR-627-5p and miR-199a-5p mimics inhibited the migration ability of SW480 cells; D: Transwell invasion assay revealed that miR-627-5p and miR-199a-5p mimics reduced the number of invasive cells; E: Flow cytometry showed that overexpression of miR-627-5p and miR-199a-5p increased the level of cell apoptosis in SW480 cells. ^bP < 0.01 vs control group; ^cP < 0.001 vs control group.