Circulating miR-627-5p and miR-199a-5p are promising diagnostic biomarkers of colorectal neoplasia

Figure 4 Elevated serum miR-627-5p and miR-199a-5p were tumor-derived. A: Comparison of tissue miR-627-5p and miR-199a-5p levels in colorectal cancer (CRC) patients, advanced colorectal adenoma (AA) patients and healthy controls (HCs). miR-627-5p was found to be sequentially decreased from normal colorectal tissues, and precancerous lesions to cancer tissues. MiR-199a-5p was markedly reduced in CRC tissues, but not significantly decreased in AA tissues; B: Comparison of miR-627-5p and miR-199a-5p levels in CRC cell lines (HCT116, RKO, and SW480) and the normal human colorectal mucosal epithelial cell line. The levels of miR-627-5p and miR-199a-5p were increased in CRC cells compared with those in FHC cells; C: Comparison of serum miR-672-5p and miR-199a-5p levels in preoperative and postoperative CRC patients. The expression levels of circulating miR-627-5p and miR-199a-5p in CRC patients were significantly lower after surgery than before surgery. ^cP < 0.001. NS: Not significant; HC: Healthy control; AA: Advanced colorectal adenoma; CRC: Colorectal cancer; AUC: Area under the curve.