DNA extraction from archived hematoxylin and eosin-stained tissue slides for downstream molecular analysis

doi:10.5662/wjm.v9.i3.32

Advanced Search

BPG is committed to discovery and dissemination of knowledge

Home / Archive / Volume 9, Issue 3

This Article

Academic Content and Language Evaluation of This Article

CrossCheck and Google Search of This Article

Academic Rules and Norms of This Article

Citation of this article

Corresponding Author of This Article

Research Domain of This Article

Article-Type of This Article

Open-Access Policy of This Article

Times Cited Counts in Google of This Article

Number of Hits and Downloads for This Article

Total Article Views (5923)

All Articles published online

The chart showing PDF series, WORD series, HTML series, Figures (1-4) series, Tables (1-2) series.

Item

Count

PDF

363

WORD

249

HTML

3005

Figures (1-4)

469

Tables (1-2)

422

Sum=4508

Publishing Process of This Article

The chart showing Browse series, Download series.

Item

Count

Browse

568

Download

847

Sum=1415

Nov 14, 2019 (publication date) through Apr 24, 2024

Times Cited of This Article

Times Cited (8)

Journal Information of This Article

Publication Name

World Journal of Methodology

ISSN

2222-0682

Publisher of This Article

Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

Basic Study

World J Methodol. Nov 14, 2019; 9(3): 32-43
Published online Nov 14, 2019. doi: 10.5662/wjm.v9.i3.32

Full Size Figure Download Figure

Figure 1 Workflow for extraction of DNA from archived H&E-stained cancer tissue slides. A: Slides selected for the extraction of DNA; B: Coverslip removal and scraping of the tissues; C: Xylene treatment for deparaffinization; D: Ethanol rehydration and stain removal; E: Tissue digestion; F: Nucleic acid purification by phase separation followed by DNA precipitation; G: Quantification of DNA using Nanodrop; H: Assessment of quality of the extracted DNA.

Citation: Ramesh PS, Madegowda V, Kumar S, Narasimha S, S R P, Manoli NN, Devegowda D. DNA extraction from archived hematoxylin and eosin-stained tissue slides for downstream molecular analysis. World J Methodol 2019; 9(3): 32-43
URL: https://www.wjgnet.com/2222-0682/full/v9/i3/32.htm
DOI: https://dx.doi.org/10.5662/wjm.v9.i3.32