Copyright
©The Author(s) 2019.
World J Methodol. Nov 14, 2019; 9(3): 32-43
Published online Nov 14, 2019. doi: 10.5662/wjm.v9.i3.32
Published online Nov 14, 2019. doi: 10.5662/wjm.v9.i3.32
Table 1 Yield and purity of the recovered DNA from various methods
| Method | Sample size, n | Tissue deparaffinization | Digestion period | Proteinase K inactivation | DNA yield range in ng/µL | DNA purity range, A260/280 |
| 1 | 20 | Xylene | 24 h | No | 7.65-45.23 | 1.42-2.01 |
| 2 | 20 | Xylene | 24 h | Yes | 7.53-33.7 | 1.34-1.94 |
| 3 | 20 | Xylene | 72 h | No | 23.68-208.31 | 1.68-1.89 |
| 4 | 20 | Xylene | 72 h | Yes | 13-149.41 | 1.54-1.93 |
| 5 | 20 | Mineral oil | 72 h | No | 11.74-86.74 | 1.41-1.74 |
- Citation: Ramesh PS, Madegowda V, Kumar S, Narasimha S, S R P, Manoli NN, Devegowda D. DNA extraction from archived hematoxylin and eosin-stained tissue slides for downstream molecular analysis. World J Methodol 2019; 9(3): 32-43
- URL: https://www.wjgnet.com/2222-0682/full/v9/i3/32.htm
- DOI: https://dx.doi.org/10.5662/wjm.v9.i3.32