Precision medicine allergy immunoassay methods for assessing immunoglobulin E sensitization to aeroallergen molecules

Table 6 Main species-specific allergen molecules of pollen origin¹ used in singleplex and multiplex immunoassays[9,74,93]

Plant source	Allergenic molecule	Biological function, comments
Specific allergen components of tree pollen origin (birch Betula verrucosa, olive tree Olea europaea, plane tree Platanus acerifolia, cypress Cupressus arizonica, etc.)
Betulaceae family	rBet v 1	PR-10 protein, heat sensitive
		CR with Bet v 1-like proteins Cor a 1, Mal d 1
		Involved in birch-hazelnuts-Rosaceae fruit syndrome
Oleaceae family	rOle e 1	Trypsin inhibitor
Platanaceae family	rPla a 1	Invertase inhibitor
	nPla a 2	Polygalacturonase
Cupressaceae family	nCup a 1	Pectate lyase
Specific allergen components of grass pollen origin (Timothy grass: Phleum pratense, Bermuda grass: Cynodon dactylon, etc.)
Poaceae family	rPhl p 1	Beta-expansin
Pooideae subfamily	rPhl p 5	Ribonuclease;
		other allergen molecules: rPhl p 2, rPhl p 6
Chloridoideae subfamily nCyn d 1 Beta-expansin
Specific allergen components of weed pollen origin (mugwort Artemisia vulgaris, ragweed Ambrosia artemisiifolia, Pellitory Parietaria judaica, plantain Plantago lanceolata, saltwort Salsola kali, goosefoot Chenopodium album, etc.)
Asteraceae family	nArt v 1	Defensin-like protein
	nAmb a 1	Pectat lyase, CR Art v 6
Urticaceae family	rPar j 2	Lipid transfer protein
Plantaginaceae family	rPla l 1	Trypsin inhibitor Ole e 1-like protein
Amaranthaceae/Chenopodiaceae family	nSal k 1	Pectin methylesterase
	rChe a 1	Trypsin inhibitor
		Ole e 1-like protein

¹n: Native purified;

r: Recombinant; Fruit hazelnut Corylus avellana (Cor a) and apple Malus domestica (Mal d) allergen molecules; Pollen Betula verrucosa (Bet v), Olea europaea (Ole e), Platanus acerifolia (Pla a), Cupressus arizonica (Cup a), Phleum pratense (Phl p), Cynodon dactylon (Cyn d), Artemisia vulgaris (Art v), Ambrosia artemisiifolia (Amb a), Parietaria judaica (Par j), Plantago lanceolata (Pla l), Salsola kali (Sal k), Chenopodium album (Che a) allergen molecules; PR: Pathogenesis-related plant proteins; CR: Cross-reactivity.