Precision medicine allergy immunoassay methods for assessing immunoglobulin E sensitization to aeroallergen molecules

Table 4 Allergen molecules of mammalian pet/pest origin¹ used in singleplex and multiplex immunoassays[9,74,105]

Allergen molecule	Biological function, comments, CR
Mammalian pets (Felis domesticus, Canis familiaris)
rFel d 1	Secretoglobin, major cat allergen, species-specific,
	sebaceous glands (testosterone influenced, production higher in males)
	Dispersed by dander (regardless of race)
	Sublingual salivary glands (saliva), lacrimal and anal glands
rFel d 2	Feline serum albumin in cat serum, dander, saliva, minor allergen
	CR nBos d 6 BSA
	CR Sus s 1 serum albumin: pork-cat syndrome involvement
rFel d 4	Feline lipocalin in cat serum, dander, saliva
	CR moderate risk with lipocalins rEqu c 1 and Can f 6
rCan f 1	Non-CR lipocalin from sublingual salivary glands (saliva) and dander
	Major allergen not influenced by hair length or hormonal status
rCan f 2	Non-CR lipocalin, major allergen in dander and saliva
	Together with rCan f 1 and rCan f 5 are species-specific markers of sensitization
nCan f 3	Canine serum albumin, found in serum, dander, saliva
	High CR with other serum albumins: rFel d 2, nEqu c 3
rCan f 4	Non-CR lipocalin major allergens
rCan f 6	(both Can f 4 and Can f 6 are dog lipocalins)
rCan f 5	Dog prostatic kallikrein, an arginine esterase,
	major allergen in male urine, dander
House mouse (Mus musculus)
nMus m 1	Lipocalin-odorant binding rodent family of MUPs

¹n: Native purified;

r: Recombinant; Cat Felis domesticus (Fel d) and dog Canis familiaris (Can f) allergen molecules; Horse Equus caballus (Equ c), cow Bos domesticus (Bos d) and domestic pig Sus scrofa (Sus s) allergen molecules; BSA: Bovine serum albumin; CR: Cross-reactivity; MUPs: Major urinary proteins.