Recombinant outer membrane protein F-B subunit of LT protein as a prophylactic measure against Pseudomonas aeruginosa burn infection in mice

Table 1 Primers and linkers used to amplify and fuse outer membrane protein F and B subunit of LT

Name	Sequence (5’-3’)	Restriction site
OprF-F	TTAA AAGCTT ATGAAACTGAAGAACACCTTAG	Hind III
OprF-R	TATA CTCGAG TTACTTGGCTTCRGCTTCT	Xho I
Linker-EAAAK-F	ATAT AAGCTT GAAGCTGCGGCAAAA ATGAAACTGAAGAAC	Hind III
Linker-EAAAK-R	ATAT CTCGAG TTACTTGGCTTCGGCTTCTACTTCGGCTTC	Xho I
LTB-F	ATAAGAATTCATGGCTCCGCAAAG	EcoR I
LTB-R	ATTAAAGCTTTTAGTTTTCCATCGAGATG	Hind III

Restriction site sequences are shown in italic.