Methodological insights into fecal microbiota transplantation: Dissecting key approaches for success

Table 1 Clinical questionnaire to select potential donors

	Question
Medical history	Any history of
	Concurrent acute, medical illness?
	Any symptoms pertaining to gastrointestinal disease (nausea/ vomiting/ pain abdomen/ diarrhea/ blood in stool)?
	Chronic gastrointestinal (GI) disease (personal or family history), including functional GI disorders, inflammatory bowel disease, celiac disease or other chronic GI disease
	Any history of chronic illness (such as diabetes/ hypertension/ heart disease/ kidney disease/ liver disease/ HIV/malignancy)?
	Acute diarrhea (in the prospective donor or his/her contacts) in the past four weeks
	Typhus or other salmonellosis
	Tuberculosis (acute or past)
	Systemic autoimmune diseases, e.g., multiple sclerosis, connective tissue disorders
	Atopic diseases
	Neurological or psychiatric diseases [depression, chronic pain (fibromyalgia, chronic fatigue syndrome, etc.)]
	Obesity (body mass index > 30)
	Known food or medication allergy
	Cancer, including GI cancer or polyposis syndrome
Travel history	Any history of high-risk travel in the past six months
Social history	Any history of
	High risk sexual behavior
	Imprisonment
	Illicit drug use
	Body modifications such as tattooing, piercing, etc., in the past six months
	Occupational activity in a hospital or other health-care institution with patient contact; occupational activity in agriculture
Family history	Any history of premature colon cancer or early onset polyposis syndromes in first-degree family members
Treatment history	Any history of
	Recent hospitalization/discharge from long-term care facilities (past 6 months)
	Antibiotic treatment in the past three months
	Blood transfusions in the past five years
	Long-term drug treatment (e.g., with antibiotics, immunosuppressants, proton pump inhibitors, chemotherapy)
	Tissue/organ transplant
	Having been a participant in any clinical trial in the past six months
	Bowel resection or gastric reduction surgery