Prednisolone inhibits SaOS2 osteosarcoma cell proliferation by activating inducible nitric oxide synthase

Figure 2 Prednisolone does not induce the formation of reactive oxygen species and antioxidants do not prevent growth inhibition. A: SaOS2 cells were treated with various concentrations of prednisolone. H₂O₂ was used as positive control. Reactive oxygen species generation was measured. Data are shown as the mean of three separate experiments ± SD. P value was calculated vs untreated cells and found not significant; B: SaOS2 cells were treated with apocynin (10 μg/mL) or trolox (40 μmol/L) in the presence of prednisolone (Prd). Viable cells were counted after 96 h. Results are shown as the mean of three separate experiments ± SD. CTR: Control.