Phenotypic profiling and characterization of stromal vascular fraction using two Novel adipose tissue processing systems

Figure 3 Flow cytometry analysis of pericytes and preadipocytes. A and B: Analysis of CD34, CD146 and Pref-1 was performed on the CD90+CD73+CD45- cells, as indicated on top of the density plots. Density plots of CD34 vs CD146 and CD34 vs Pref-1, respectively. Pericytes subpopulation (CD90+CD73+CD45- CD34-CD146+) is highlighted by green boxed regions, while preadipocyte subpopulation (CD90+CD73+CD45- CD34+ Pref-1+), characterized by Pref-1 and CD34 co-expression, is highlighted by blue boxed areas; C: Density plots of CD146 vs Pref-1, indicating their partial co-expression. Results are representative of at least three independent experiments for each isolation procedure.