Phenotypic profiling and characterization of stromal vascular fraction using two Novel adipose tissue processing systems

Figure 2 Flow cytometry analysis of adipose-derived stromal cells subpopulation. The analysis shown in this figure was carried out on CD90+CD73+ cells, which were negative for CD45 expression, as indicated on top of the density plots. A: The percentage of CD271- and CD34-positive cells in adipose-derived stromal cells isolated by Lipoaspirate, Lipogems^®, and Body-Jet^® eco procedures is reported; B: Cells positive for CD31 and CD34; C: A wide range of coexpression with CD34 is observed. These cells are also HLA-DR-positive; D: Negative for CD271, as indirectly demonstrated. For greater clarity, red boxed regions highlight the endothelial progenitor cells. Results are representative of at least three independent experiments for each isolation procedure.