Changes in urinary excretion of water and sodium transporters during amiloride and bendroflumethiazide treatment

Table 1 Experimental procedures

	Before the study day				On the study day
	Day-4	Day-3	Day-2	Day-1	6:00-8:00	8:00-08:30	8:30-09:00	9:00-9:30	9:30-10:00	10:00-10:30	10:30-11:00	11:00-11:30	11:30-12:00	12:00-12:30	12:30-13:00	13:00-13:30
Periods										Baseline		Infusion			Post Infusion
Time								0	30	60	90	120	150	180	210	240
Diet	x	x	x	x
Study drug	xx	xx	xx	xx	x
24-h BP				x------	-----x
24-h urine				x------	-----x
IV access						x
Weight						x					x		x			x
Water load						x--------------------------------------------------------------------------- 175 mL every 30 min ----------------------------------------------------------------------------------------------- x
Urine sample							(x)	x	x	x	x		x	x	x	x
Blood samples								x	x	x	x		x	x	x	x
Blood pressure						x		x	x	x	x		x	x	x	x
51Cr-EDTA								x ------------------------------------------------------------------------------------------------------------------------------------------------------------------ x
IV. fluid												x-----------------x
App.Ton											x		x
BIS						x					x		x			x

24-h BP: 24-h ambulatory blood pressure measurements; 24-h urine: 24-h urine collection; App.Ton: Applanation tonometry; BIS: Bioimpedance spectroscopy.