Delivery strategies for novel vaccine formulations

Figure 1 Selective targeting of antigens to dendritic cells. Schematic diagram of the new bacteriophage vector displaying at the N-terminus of the pIIIgene a single chain antibody fragment (scFv) binding the mouse dendritic cells DEC-205 receptor and an antigenic peptide as N-terminal fusion to the major coat pVIII protein. The scFv is composed of heavy (VH) and light (VL) chain variable regions of the mouse monoclonal antibody NLDC-145, assembled with a (Gly4Sert)3 linker to yield a 750 bp long single-chain fragment. g: Gene; p: Protein.

Figure 2 Schematic image of the E2 acetyltransferase display system derived from the Bacillus stearothermophilus pyruvate dehydrogenase complex. A: The E2 chain as it occurs in the native pyruvate dehydrogenase complex. Twenty trimers of the E2 polypeptide chain form a pentagonal dodecahedron (60-mer) with icosahedral symmetry; B: Representation of the E2 core displaying the HIV-1 Envelope (Env) third hypervariable region V3 on the surface of the scaffold. LIP: Lypoil domain; PSBD: Peripheral subunit-binding domain; CD: Catalytic domain; E2DISP: E2 acetyltransferase display system.