Electroacupuncture ameliorates visceral hypersensitivity and negative emotions by regulating paraventricular hypothalamic nucleus and colonic corticotropin-releasing factor signaling

Figure 2 Effects of electroacupuncture at different acupoints on diarrhoeal irritable bowel syndrome. A: Schematic representation of the electroacupuncture positions of Baihui (GV20), Tianshu (ST25) and Dachangshu (BL25); B: Flowchart diagram of electroacupuncture acupoints in mice; C: Paths travelled by the mice in each group in the open-field test; D: Statistical graphs of distance in center and time in center of the mice in the open-field test; E: Statistical graph of saline injections in each group of mice in the colorectal dilatation test; n = 6 mice in each group, model group vs control group, model + GV20/model + ST25 + BL25/model + ST25 + BL25 + GV20 group vs model group; ^aP < 0.05, ^bP < 0.01; NS: Not significant; GV20: Baihui; ST25: Tianshu; BL25: Dachangshu; EA: Electroacupuncture.