Increased expression of the neuroplastin 65 protein is involved in neurofibrillary tangles and amyloid beta plaques in Alzheimer’s disease

Figure 6 Neuroplastin 65 colocalized with the neurofibrillary tangles and amyloid beta plaques in Alzheimer’s disease brain. A: The double immunostaining microphotograph of neuroplastin 65 (NP65) with AT-8 (a marker for neurofibrillary tangles) showed that NP65-positive puncta partially colocalized with phosphorylated-microtubule-associated protein tau (yellow, indicated by arrow) in the entorhinal cortex of Alzheimer’s disease brain, scale bar = 30 μm; the left panel shows a higher power view in the rectangle frame, scale bar = 10 μm; B: The double immunostaining microphotograph of NP65 with 6E10 (a marker for amyloid beta) exhibited that NP65-positive puncta partially colocalized with amyloid beta plaques (yellow, indicated by arrow) in the cornu ammonis 3 region of Alzheimer’s disease brain, scale bar = 200 μm; The left panel shows a higher power view in the rectangle frame, scale bar = 30 μm. The nucleus was stained with 4’,6-diamidino-2-phenylindole (blue). NP65: Neuroplastin 65; EC: Entorhinal cortex; DG: Dentate gyrus; SM: Stratum moleculare; SG: Stratum granulosum; Spl: Stratum plexiforme; DAPI: 4’,6-diamidino-2-phenylindole.