An inhibitor of HIF-α subunit expression suppresses hypoxia-induced dedifferentiation of human NSCLC into cancer stem cell-like cells

Figure 3 Effect of hypoxia on the expression of differentiation-associated genes. The cells were cultured under normoxic (N) or hypoxic (H) conditions for 5 d. A: Expression of differentiation-associated genes in A549 and QG56 cells; B: Normalized expression levels of the genes in A549; C: Normalized expression levels of the genes in QG56 cells; D: Time-course of the expression of SP-C in A549 cells; E: The expression of CC10 and AQP5 in QG56 cells; The expression level of each gene was normalized to that of β-Actin (ACTB); Data are shown as fold-change relative to normoxia (normoxia values set to equal 1), ^aP < 0.05 (n = 3); The cells were cultured under hypoxic conditions for the indicated periods.