Evaluation of urinary extracellular vesicles and microRNAs to diagnose urogenital tuberculosis

doi:10.5493/wjem.v15.i3.105208

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Sep 20, 2025 (publication date) through Aug 25, 2025

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World Journal of Experimental Medicine

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Exp Med. Sep 20, 2025; 15(3): 105208
Published online Sep 20, 2025. doi: 10.5493/wjem.v15.i3.105208

Table 1 Oligonucleotides used in this study

Primer set name	Reverse transcriptase reaction primer (5’ to 3’)	Real-time quantitative PCR primer (5’ to 3’)
miRNA-155-5p	GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACAACCCC	Forward: TCGCTTTAATGCTAATCGTGATA
miRNA-155-5p	GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACAACCCC	Reverse: CCAGTGCAGGGTCCGAGGTA
miRNA-26a-5p	GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACAGCCTA	Forward: TCGCTTTCAAGTAATCCAGGA
miRNA-26a-5p	GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACAGCCTA	Reverse: CCAGTGCAGGGTCCGAGGTA
miRNA-29a-3p	GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACTAACCG	Forward: TCGCTTAGCACCATCTGAAAT
miRNA-29a-3p	GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACTAACCG	Reverse: CCAGTGCAGGGTCCGAGGTA
U6	GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACAAAATATGGAACGCTT	Forward: AGAAGATTAGCATGGCCCCTG
U6		Reverse: CCAGTGCAGGGTCCGAGGTA

PCR: Polymerase chain reaction; MiRNAs: MicroRNA.

Citation: Das P, Chaudhary DK, Mishra R, Tiwari S. Evaluation of urinary extracellular vesicles and microRNAs to diagnose urogenital tuberculosis. World J Exp Med 2025; 15(3): 105208
URL: https://www.wjgnet.com/2220-315X/full/v15/i3/105208.htm
DOI: https://dx.doi.org/10.5493/wjem.v15.i3.105208