Reactive oxygen species elevations in human immune cell subsets during sepsis are mitigated by norepinephrine and N-acetylcysteine

Figure 2 Reactive oxygen species levels are increased septic patients in CD3+ and CD14+ cells. A–D: Representative histograms of intracellular reactive oxygen species (ROS) fluorescence in CD3+ T cells, CD14+ monocytes, CD15+ granulocytes and CD19+ B cells, from 1 control and 1 septic patient; E–H: Quantification of ROS levels (mean ± SD) in each subset from 6 control and 6 septic patients. While CD3+ and CD14+ cells exhibited significantly elevated ROS levels in sepsis, CD19+ and CD15+ cells did not show statistically significant differences; I–K: Plasma concentrations of interleukin (IL)-6, tumor necrosis factor-α, and IL-10 measured in 13 control and 12 septic patients. Statistical analysis: Unpaired two-tailed Student’s t-test. ^aP < 0.05, ^cP < 0.001. IL: Interleukin; ROS: Reactive oxygen species.